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BMP7 protein, human

BMP7 (Bone Morphogenetic Protein 7) is a member of the transforming growth factor-beta (TGF-beta) superfamily of proteins.
It plays a crucial role in bone and cartilage development, as well as tissue repair and regeneration.
BMP7 has been studied extensively for its potential therapeutic applications in bone-related disorders, kidney diseases, and other conditions.
Researchers can leverage PubCompare.ai to optimize their BMP7 protein research by accessing the most reliable and effective protocols from scientific literature, pre-prints, and patents.
This AI-driven platform enhances reproducibility and accuracy, helping scientists identify the best approaches for their BMP7 protein studies and improve their research outcomes.

Most cited protocols related to «BMP7 protein, human»

1
Liver Organoid Differentiation and Functional Assay
Cited 13 times
Liver organoids were seeded and kept 7–10 days under the liver medium explained above (EM, expansion medium) supplemented with BMP7 (25 ng/ml). Then, the cultures were split and seeded accordingly in this EM supplemented with BMP7 for at least 2–4 days. Then, medium was changed to the differentiation medium (DM): AdDMEM/F12 medium supplemented with 1% N2 and 1% B27 and containing EGF (50 ng/ml), gastrin (10 nM, Sigma), HGF (25 ng/ml), FGF19 (100 ng/ml), A8301 (500 nM), DAPT (10 uM), BMP7 (25 ng/ml), and dexamethasone (30 uM). Differentiation medium was changed every 2–3 for a period of 11–13 days.
To assess hepatocyte function, culture medium was collected 24 hr after the last medium change. Functional studies were performed in the collected supernatant or in whole organoids, as described in the Extended Experimental Procedures.
Huch M., Gehart H., van Boxtel R., Hamer K., Blokzijl F., Verstegen M.M., Ellis E., van Wenum M., Fuchs S.A., de Ligt J., van de Wetering M., Sasaki N., Boers S.J., Kemperman H., de Jonge J., Ijzermans J.N., Nieuwenhuis E.E., Hoekstra R., Strom S., Vries R.R., van der Laan L.J., Cuppen E, & Clevers H. (2015). Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver. Cell, 160(1-2), 299-312.
Publication 2015
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol BMP7 protein, human Dexamethasone Gastrin Hepatocyte Liver Organoids
2
Validating MHG Classifier Across Platforms
Cited 8 times
Although the MHG group was defined independently of the clinical trial data, we validated it further on another independent and recently published data set4 (European Genome-Phenome Archive study accession EGAS00001002606) by using the core set of 624 patients whose gene expression profiles were examined in that study by RNA sequencing. Adaptation of our classifier to these data was straightforward because the classifier was designed and tested18 (link) for cross-platform applicability. We note, however, that there are no diagnoses of BL in this data set, which could have a minor effect on the overall normalization, and that in this data set four classifier genes (BMP7, TCL6, SOX11, and C7orf10) had very low estimated expression levels from the RNA sequencing data. The classifier, therefore, was retrained using the original training data,18 (link) with the gene set reduced by these four genes for application to this data set. COO classification was that provided by the authors. Analysis of mutation frequencies in this data set used the 150 identified driver genes from the original article filtered by at least 5% mutation frequency in at least one subgroup and significantly different frequency (Fisher’s exact P < .05) between any two groups of MHG, GCB, and ABC.
Sha C., Barrans S., Cucco F., Bentley M.A., Care M.A., Cummin T., Kennedy H., Thompson J.S., Uddin R., Worrillow L., Chalkley R., van Hoppe M., Ahmed S., Maishman T., Caddy J., Schuh A., Mamot C., Burton C., Tooze R., Davies A., Du M.Q., Johnson P.W, & Westhead D.R. (2018). Molecular High-Grade B-Cell Lymphoma: Defining a Poor-Risk Group That Requires Different Approaches to Therapy. Journal of Clinical Oncology, 37(3), 202-212.
Publication 2018
Acclimatization BMP7 protein, human Diagnosis Europeans Genes Genome Patients SOX11 protein, human Transcription, Genetic
3
Enhancing Adipocyte Mitochondrial Activity
Cited 8 times
Alternatively, to induce increased UCP1 expression, mitochondrial activity and fuel utilization in mature adipocytes, cells can be pretreated with BMP7 in differentiation medium prior to adipogenic differentiation as described below (9 (link)–11 (link)).

Add 3.3 nM BMP7 or similar volume of vehicle to the differentiation medium.

Once cells reach confluency, aspirate growth media and add differentiation medium containing BMP7 or vehicle to cells.

Treat cells with BMP7 in differentiation medium for six days. Add fresh differentiation medium every three days.

At day 7, start adding induction media to cells.

Change induction medium every three days for 12 days.

Shamsi F, & Tseng Y.H. (2017). Protocols for generation of immortalized human brown and white preadipocyte cell lines. Methods in molecular biology (Clifton, N.J.), 1566, 77-85.
Publication 2017
Adipocytes Adipogenesis BMP7 protein, human Cells Mitochondrial Inheritance UCP1 protein, human
4
Generation and Maintenance of Murine and Human Nephron Progenitor Cells
Cited 8 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2016
A-83-01 Animals BMP7 protein, human Cells Chir 99021 Ethics Committees, Research Fetus Fibroblast Growth Factor 2 Heparin Homo sapiens Infant, Newborn Institutional Animal Care and Use Committees Kidney LDN 193189 mitogen-activated protein kinase 3, human Mus NGFR protein, human NPSR1 protein, human Receptors, Antigen, B-Cell TACSTD1 protein, human Y 27632
5
Antibody-based analysis of kidney injury
Cited 6 times
Monoclonal antibody for E–cadherin was purchased from BD Biosciences. Mac–1 antibody was purchased from AbD Serotec. F4/80 and BMP7 antibody was purchased from Abcam. p–Smad1/5 antibody was purchased from Cell Signaling Technology. Alk3 and E–cadherin antibodies were purchased from Santa Cruz Biotechnology. For western blot analyses, Alk3 antibody was purchased from EMD Millipore. Measurements of BUN were performed using the QuantiChrom™Urea Assay Kit (BioAssay System) or DIUR–500 QuantiChrom™ Urea Assay Kit (Gentaur). Measurements of urine albumin and urine creating were performed using the QuantiChrom™ Assay for albumin (DIAG-500) and creatinine (DICT-250) Kit (BioAssay System). Details for morphormetric analyses of histological findings are listed in the online method section.
Sugimoto H., LeBleu V.S., Basukonda D., Keck P., Taduri G., Bechtel W., Okada H., Carlson W J.r., Bey P., Rusckowski M., Tampe B., Tampe D., Kanasaki K., Zeisberg M, & Kalluri R. (2012). Activin–like kinase–3 activity is important for kidney regeneration and reversal of fibrosis. Nature medicine, 18(3), 396-404.
Publication 2012
Albumins Antibodies Biological Assay BMP7 protein, human Cadherins CDH1 protein, human Creatinine Immunoglobulins Macrophage-1 Antigen Monoclonal Antibodies Urea Urea Nitrogen, Blood Urine Western Blot

Most recents protocols related to «BMP7 protein, human»

1
BMP-7 Mimicking Peptide Enhances Cartilage ECM
Not available on PMC !

Example 7

Cartilage explants obtained from 2 patients were cultured for 14 days in the presence of BMP-7 (1 nM) or BMP-7 mimicking peptide GYAAYYSEGESAFPLNSYMN (SEQ ID NO: 8) at 10 nM. Glycosaminoglycans (GAGs), an important component of the extracellular matrix (ECM), were stained with Safranin-O (in red) and other tissues are counterstained with Fast green (in green/blue).

Both patients showed an increased Safranin-O intensity in BMP7 and peptide GYAAYYSEGESAFPLNSYMN (SEQ ID NO: 8) treated explants compared to control.

These results are in line with the effects described above and show the BMP-7 mimicking bioactivity of the peptides according to the invention.

US11872264B2. Method for the treatment or prevention of osteoarthritis (2024-01-16). Chondropeptix B.V. [NL]. Inventors: Tim Johannes Maria Welting [NL], Marjolein Maria Johanna Caron [NL].
Patent 2024
BMP7 protein, human Cartilage Extracellular Matrix Fast Green Glycosaminoglycans Patients Peptides safranine T Tissues
2
Isolation and Characterization of Nucleus Pulposus Cells from Diabetic Rats
The NP tissues from control and STZ-induced T1DM rats were rinsed with PBS (Gibco, Grand Island, NY, USA). After being cut into blocks, the samples were detached with 0.25 mg/mL II collagenase (Gibco) for 6 h, and subsequently filtered with a filter mesh size of 70 μm. After washing in PBS and centrifugation, the isolated NPCs were cultured in DMEM/F12 medium containing 15% FBS and 1% penicillin–streptomycin (Gibco). After identification using NPC fluorescence-labeled antibodies (CD24, #BS-4891R, dilution ratio of 1:20, Invitrogen; KRT18, #MA1-06326, dilution ratio of 1:20; Invitrogen) (Additional file 1: Fig. S1A, B), the NPCs at passage 2 were used for subsequent experiments.
NPCs at the logarithmic phase of growth were detached with trypsin and seeded into 6-well plates (density of 1 × 105 cells/well), followed by incubation for 24 h. Upon obtaining 75% confluence, the NPCs were transduced with lentiviruses (Shanghai GeneChem Co., Ltd., Shanghai, China) as instructions described.

Control group (primary NPCs from normal rats).

STZ (primary NPCs from STZ-induced rats).

STZ + oe-NC (primary NPCs from STZ-induced rats transduced with oe-NC).

STZ + oe-BMP7 (primary NPCs from STZ-induced rats transduced with oe-NC).

STZ + oe-BMP7 + DMSO (primary NPCs from STZ-induced rats transduced with oe-BMP7 and treated with DMSO).

STZ + oe-BMP7 + 4’MR (primary NPCs from STZ-induced rats transduced with oe-BMP7 and treated with 4’MR [10 μM, HY-N2485, MedChemExpress]).

Yu X.J., Wang Y.G., Lu R., Guo X.Z., Qu Y.K., Wang S.X., Xu H.R., Kang H., You H.B, & Xu Y. (2023). BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity. Molecular Medicine, 29, 30.
Publication 2023
BMP7 protein, human Centrifugation Collagenase Culture Media Fluorescent Antibody Technique KRT18 protein, human Lentivirus mutalipocin II Penicillins Rattus norvegicus Strains Streptomycin Sulfoxide, Dimethyl Technique, Dilution Tissues Trypsin
3
Investigating BMP7 in STZ-Induced IDD Rats
The STZ-induced IDD rats were randomly divided into 5 groups (n = 12).

STZ group (untreated STZ-induced rats).

STZ + oe-NC group (STZ-induced rats were injected with over-expression negative control (NC) lentivirus via tail vein).

STZ + oe-BMP7 group (STZ-induced rats were injected with lentivirus over-expressing BMP7 via tail vein).

STZ + oe-BMP7 + dimethyl sulfoxide (DMSO) group (STZ-induced rats were injected with lentivirus over-expressing BMP7 and 1% DMSO via tail vein).

STZ + oe-BMP7 + 4’-Methoxyresveratrol (4’MR) group (STZ-induced rats were injected with lentivirus over-expressing BMP7 and NLRP3 agonist 4’MR (to activate NLRP3) via tail vein).

One week after STZ injection, STZ-induced rats, except for group 1, were injected with 100 μL lentivirus via tail vein with a viral titer of 5 × 108 TU. Two days later, STZ-induced rats in group 4–5 were injected with 100 μL 4’MR (25 mg/kg, HY-N2485, MedChemExpress, Shanghai, China) or 1% DMSO (100 μL, HY-Y0320, MedChemExpress), once every week. Afterwards, the rats were euthanized on the 8th week after STZ injection, and Co6–Co7 discs and nucleus pulposus (NP) tissues were isolated from rats for subsequent experiments.
Yu X.J., Wang Y.G., Lu R., Guo X.Z., Qu Y.K., Wang S.X., Xu H.R., Kang H., You H.B, & Xu Y. (2023). BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity. Molecular Medicine, 29, 30.
Publication 2023
BMP7 protein, human Lentivirus Nucleus Pulposus Rattus norvegicus Sulfoxide, Dimethyl Tail Tissues Veins
4
Immunofluorescent Analysis of FGF-21 and BMP7
Cited 1 time
Skin specimens were fixed in 4% paraformaldehyde and were then dehydrated and embedded in paraffin. Paraffin sections were successfully dewaxed in different gradients of the dewaxing solution, absolute ethanol, and distilled water. The dewaxed sections were placed in EDTA antigen repair buffer (pH 8.0) for antigen repair and blocked with a 10% donkey serum block. Primary antibodies were then added: anti-rabbit-FGF-21 (1:200) and BMP7 antibody (1:200), and incubated overnight at 4°C. On the next day, the slides were washed three times with PBS (pH 7.4) in a rocker device, the objective tissue was covered with a secondary antibody (responding appropriately to the primary antibody in the species), and was incubated at room temperature for 50 min in dark conditions. Finally, DAPI dye solution was added and incubated for 10 min. The images were observed and collected under a fluorescence microscope (GE Bioscience, Newark, NJ, USA).
Diao X., Yao L., Wang X., Li S., Qin J., Yang L., He L, & Zhang W. (2023). Hair Follicle Development and Cashmere Traits in Albas Goat Kids. Animals : an Open Access Journal from MDPI, 13(4), 617.
Publication 2023
Antibodies Antigens BMP7 protein, human Buffers DAPI Edetic Acid Equus asinus Ethanol fibroblast growth factor 21 Immunoglobulins Medical Devices Microscopy, Fluorescence Paraffin Paraffin Embedding paraform Rabbits Serum Skin Tissues
5
Skin mRNA Expression Profiling
FGF2, FGF21, BMP4, and BMP7 mRNA expression levels in the skin were measured using Q-PCR. The total RNA from the skin tissue was extracted using TRIzol (Takara, Dalian, China). All primer sequences were determined using established GenBank sequences, which are listed in Table 2. RNA was reverse transcribed to cDNA using a cDNA Synthesis Kit (Servicebio, Beijing, China). Real-time qPCR was performed on an ABI7500 system (Applied Biosystem, CA, USA) using a qPCR Detection kit (SYBR Green) (Servicebio, Beijing, China). Data were processed using ABI7500 Software v. 2.0.6 (Applied Biosystem, CA, USA). The RT-PCR analysis was performed using the 2−ΔΔCT method.
Diao X., Yao L., Wang X., Li S., Qin J., Yang L., He L, & Zhang W. (2023). Hair Follicle Development and Cashmere Traits in Albas Goat Kids. Animals : an Open Access Journal from MDPI, 13(4), 617.
Publication 2023
Anabolism BMP7 protein, human Bone Morphogenetic Protein 4 DNA, Complementary Fibroblast Growth Factor 2 fibroblast growth factor 21 Oligonucleotide Primers Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Skin SYBR Green I trizol

Top products related to «BMP7 protein, human»

1
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
2
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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BMP4 is a recombinant protein that belongs to the transforming growth factor-beta (TGF-β) superfamily. It is a key regulator of embryonic development and plays a crucial role in various cellular processes, including cell growth, differentiation, and tissue patterning.
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
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Noggin is a protein that functions as a bone morphogenetic protein (BMP) antagonist. It is used in cell culture and biochemical applications to inhibit BMP signaling.

More about "BMP7 protein, human"

Bone Morphogenetic Protein 7 (BMP7), also known as Osteogenic Protein-1 (OP-1), is a member of the Transforming Growth Factor-Beta (TGF-β) superfamily of proteins.
This crucial signaling molecule plays a vital role in the development and regeneration of bone, cartilage, and various other tissues.
BMP7 has been extensively studied for its therapeutic potential in treating bone-related disorders, kidney diseases, and other conditions.
The BMP7 protein is produced by a variety of cell types, including osteoblasts, chondrocytes, and cells in the kidney and other organs.
It functions by binding to specific cell surface receptors, which then activate intracellular signaling pathways that regulate gene expression and cellular processes.
BMP7 is particularly important for the differentiation of mesenchymal stem cells into osteoblasts, the cells responsible for bone formation.
In addition to its role in bone and cartilage development, BMP7 has been found to have a protective effect on the kidneys, making it a potential therapeutic target for conditions such as chronic kidney disease and diabetic nephropathy.
Researchers have also investigated the use of BMP7 in wound healing, nerve regeneration, and the treatment of various other medical conditions.
To study the effects of BMP7, researchers often utilize techniques such as cell culture, gene expression analysis, and animal models.
Common reagents used in BMP7 research include TRIzol for RNA extraction, Dexamethasone for osteogenic differentiation, Lipofectamine 2000 for transfection, and FBS for cell culture.
Additionally, related proteins like BMP4 and Noggin have been studied in the context of BMP7 signaling and regulation.
By leveraging the insights and protocols available on platforms like PubCompare.ai, researchers can optimize their BMP7 protein studies, enhance reproducibility, and improve their research outcomes.
This AI-driven platform provides access to the most reliable and effective protocols from scientific literature, pre-prints, and patents, empowering researchers to identify the best approaches for their specific BMP7-related investigations.