Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Amino Acid > Casein peptone

Casein peptone

Casein peptone is a nutrient-rich complex of peptides derived from the enzymatic hydrolysis of casein, a major milk protein.
This substance serves as a versatile growth medium component, supporting the cultivation of a wide range of microorganisms in laboratory settings.
Casein peptone provides a rich source of amino acids, peptides, and other essential nutrients required for microbial proliferation and metabolism.
Researchers leveraging casein peptone in their work can optimize reproducibility and accuracy through PubCompare.ai's AI-driven platform, which helps locate relevant protocols from literature, preprints, and patents, while enabling intelligent comparisons to identify the most optimal protocols and products.
This tool can enhance the quality and efficieny of casein peptone research, improving overall outcomes.

Most cited protocols related to «Casein peptone»

1
Fermentation Medium Preparation and Screening
The fermentation medium for primary screening was FM 3 [67 ]: soluble starch, 20 g; soy powder, 15 g; yeast extract, 5 g; peptone, 2 g; CaCO3, 4 g; sea salts, 18 g in 1 L distilled water, pH 7.2~7.4. Two media were used for re-screening, namely FM 2 [68 ]; Glucose, 10 g; soluble starch, 30 g; yeast powder, 2 g; casein, 4 g; K2SO4, 8 g; MOPS (3-[N-morpholino]propanesulfonic acid), 5 g; sea salts, 18 g; distilled water, 1 L; pH 7.2–7.4; and FM 17 [69 ]; Glucose, 20 g; yeast powder, 5 g; casein, 5 g; KNO3, 15 g; CaCO3, 4 g; sea salts, 18 g; distilled water, 1 L; pH 7.2–7.4. All three media were autoclaved at 121 °C for 20 min.
Each of 2,041 purified isolates were transferred to a test tube (30 mm×200 mm) which contained 20 mL of the relevant fermentation medium, and cultured at 200 rpm, at an angle of 45º, for 7–10 days at 28 °C. Crude extracts were prepared by adding 60 mL of methanol to each of the cultures and the extraction allowed to proceed for 2 weeks. One mL fractions of the resultant extracts were transferred to wells in deep 96-well plates, vacuum dried at 60 °C, dissolved in 200 μL DMSO and used in the biochemical screens and for cytotoxicity assay against HCT-116 cells.
Hong K., Gao A.H., Xie Q.Y., Gao H., Zhuang L., Lin H.P., Yu H.P., Li J., Yao X.S., Goodfellow M, & Ruan J.S. (2009). Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China. Marine Drugs, 7(1), 24-44.
Full text: Click here
Publication 2009
Acids Biological Assay Carbonate, Calcium Caseins Complex Extracts Cytotoxin Fermentation Glucose HCT116 Cells Methanol morpholinopropane sulfonic acid Morpholinos Peptones Powder Salts Starch Sulfoxide, Dimethyl Vacuum Yeast, Dried
2
Characterization of Actinobacterial Isolates
Morphological, biochemical, culture and physiological characterization of the actinobacterial isolates of Minnie Bay were performed as recommended by the International Streptomyces Project (ISP) which were described by Shirling and Gottileb [18 ]. Microscopic study was performed with cover slip culture and cellophane method [19 ]. Formation of aerial, substrate mycelium and spore arrangements on mycelium were monitored under a phase contrast microscope (Nikon ECLIPSE E600, USA) at 100× magnification. Culture characteristics such as growth, coloration of aerial and substrate mycelia, formation of soluble pigment were investigated in eight different media including SCA, nutrient agar, yeast malt agar (ISP-2), oat meal agar (ISP-3), inorganic salt agar (ISP-4), glycerol-asparagine agar (ISP-5), peptone yeast extract agar (ISP-6) and tyrosine agar (ISP-7) with the procedures as recommended by ISP. Biochemical characterization, namely, Gram’s reaction, MR-VP, H2S production, nitrate reduction, oxidase, catalase, urease, starch, casein and gelatin hydrolysis, blood hemolysis, TSI, citrate utilization, esculin and hippurate hydrolysis was also performed as suggested by ISP. Physiological characterization such as, effect of pH (5–11), growth range in NaCl (5-30%) and survival at 50°C was also evaluated. Capability of the isolates to utilize various carbon sources was performed in ISP-2 agar medium with phenol red as indicator [20 ]. Carbon sources viz., fructose, lactose, starch, dextrose, rhamnose, mannitol, maltose, adonitol, arabinose and raffinose were used in this study. Identification of the isolates was made with reference to Bergey’s manual of Systematic Bacteriology [21 ] and Waksman [22 ].
Meena B., Rajan L.A., Vinithkumar N.V, & Kirubagaran R. (2013). Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts. BMC Microbiology, 13, 145.
Full text: Click here
Publication 2013
Agar Arabinose Asparagine Blood Carbon Caseins Catalase Cellophane Citrates E-600 Esculin Fructose Gelatins Glucose Glycerin Hemolysis hippurate Hydrolysis Lactose Maltose Mannitol Microscopy Microscopy, Phase-Contrast Mycelium Nitrates Nutrients Oxidases Peptones physiology Pigmentation Raffinose Rhamnose Ribitol Salts Sodium Chloride Spores Starch Streptomyces Tyrosine Urease Yeasts
3
Community-Acquired Bacteremia Screening Protocol
We recruited all patients admitted with suspected community-acquired bacteremia if they gave informed verbal consent. No formal definition was used and recruitment was at the discretion of the responsible physician. History and clinical examination were recorded on standard forms. A venous blood sample was drawn aseptically for 2 blood cultures (based on tryptic hydrolysate of casein and soy peptone) (Pharmaceutical Factory No 2, Vientiane). The volume of blood taken was 5 mL/bottle for adults (≥ 15 years), 2 ml/bottle for children > 1 year, and 1 mL/bottle for infants. The ratio of blood to media was 1:10.
PHETSOUVANH R., PHONGMANY S., SOUKALOUN D., RASACHAK B., SOUKHASEUM V., SOUKHASEUM S., FRICHITHAVONG K., KHOUNNORATH S., PENGDEE B., PHIASAKHA K., CHU V., LUANGXAY K., RATTANAVONG S., SISOUK K., KEOLOUANGKOT V., MAYXAY M., RAMSAY A., BLACKSELL S.D., CAMPBELL J., MARTINEZ-AUSSEL B., HEUANVONGSY M., BOUNXOUEI B., THAMMAVONG C., SYHAVONG B., STROBEL M., PEACOCK S.J., WHITE N.J, & NEWTON P.N. (2006). CAUSES OF COMMUNITY-ACQUIRED BACTEREMIA AND PATTERNS OF ANTIMICROBIAL RESISTANCE IN VIENTIANE, LAOS. The American journal of tropical medicine and hygiene, 75(5), 978-985.
Publication 2006
Adult Bacteremia BLOOD Blood Culture Blood Volume casein hydrolysate Child Infant Patients Peptones Pharmaceutical Preparations Physical Examination Physicians Trypsin Veins
4
Comprehensive Milk Composition Analysis
The following parameters were determined on each milk sample: lactose and fat by means of the infrared analysis with Milko-Scan (Foss Electric, Hillerød, Denmark), pH by a potentiometer with a specific electrode (Crison Instruments, Barcelona, Spain), and somatic cells and total bacterial count by fluoro-opto-electronic and flow cytometry methods with Fossomatic 250 and BactoScan FC, respectively (Foss Electric, Hillerød, Denmark). Dry matter and ash were determined by drying at 102 °C for 7 hours and calcination in the muffle at 530 °C, respectively, and from ash solubilized in HCl (2N). The total contents of Ca, Mg, Na, K, Zn, Fe and Cu were assessed by Atomic Absorption Spectrometry (AAS) and phosphorus (P) was assessed with the colorimetric method, according to Malacarne et al. [12 (link)]. Total nitrogen (TN) on whole milk, non-casein nitrogen (NCN) on milk acid whey at pH 4.6, and non-protein nitrogen (NPN) on trichloroacetic acid (TCA) filtrated and obtained from milk after treatment with TCA (120 g/L) were determined with the Kjeldahl method. Proteose-peptone N (PPN) was measured on acid whey obtained, according to van Boeckel and Crijns [13 ]. From these nitrogen fractions, the following parameters were calculated: casein nitrogen (CN = TN – NCN), true protein nitrogen (TPN = TN−NPN), crude protein (TN × 6.38), casein (CN × 6.38), true protein (TPN × 6.38), and proteose-peptones (PPN × 6.38). The Kjeldahl method was performed using a DK6 Digestor and UDK126A Distiller (VELP Scientifica, Usmate, Italy), according to the Association of Official Analytical Chemists (AOAC) standards [14 ,15 ,16 ].
Chloride (Cl−) was assessed by titration with silver nitrate (AgNO3) using the volumetric method of Charpentier-Volhard.
All glasses and polyethylene tubes used for collection, storage, and analysis of samples were previously washed with 2N hydrochloric acid (Carlo Erba reagent, Milano, Italy) solution. All solutions were prepared using two-time distilled water (conductivity < 4.3 µS/cm).
Malacarne M., Criscione A., Franceschi P., Bordonaro S., Formaggioni P., Marletta D, & Summer A. (2019). New Insights into Chemical and Mineral Composition of Donkey Milk throughout Nine Months of Lactation. Animals : an Open Access Journal from MDPI, 9(12), 1161.
Full text: Click here
Publication 2019
Acids Aftercare Caseins Chlorides Colorimetry Counts, Bacterial Diploid Cell Electric Conductivity Electricity Eyeglasses Flow Cytometry FOS protein, human Gene, THRA Hydrochloric acid Lactic Acid Lactose Milk, Cow's Nitrogen Phosphorus Polyethylene Proteins proteose-peptone Radionuclide Imaging Silver Nitrate Spectrophotometry, Atomic Absorption Titrimetry Trichloroacetic Acid Whey
5
Evaluation of Probiotic Strains for Dairy Applications
Streptococcus thermophilus TH-4, Lactobacillus delbrueckii subsp. bulgaricus YC-X11 (isolated from FD-DVS YC-X11 Yo-Flex® yogurt culture), Lb. delbrueckii subsp. bulgaricus CH-2, Lb. acidophilus LA-5, and Bifidobacterium animalis subsp. lactis BB-12 were kindly supplied by Chr. Hansen (Hørsholm, Denmark). Bifidobacterium breve M-16 V was obtained from Morinaga Milk Industry (Tokyo, Japan). Streptococcus thermophilus DSM 20479 was received from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Lactobacillus acidophilus NCAIM B.02085 and Lb. casei NCAIM B.01137 were provided by the National Collection of Agricultural and Industrial Microorganisms (Budapest, Hungary). Lactococcus lactis subsp. lactis ATCC 19435 and Leuconostoc mesenteroides subsp. dextranicum ATCC 19255 were purchased from the American Type Culture Collection (Manassas, Virginia, USA). Lactobacillus casei HDRI-R and Lc. lactis subsp. lactis biovar. diacetylactis VK-256 were obtained from the culture collection of the Hungarian Dairy Research Institute (Mosonmagyaróvár, Hungary). All these cultures were tested for purity by Gram stain and observation of cell morphology under a KF 2 ICS transmitted light microscope (Carl Zeiss Microscopy, Jena, Germany).
All strains were maintained at −30 °C using the MicrobankTM low-temperature microbial preservation system (Pro-Lab Diagnostics, Neston, United Kingdom). Organisms were retrieved from cryovials containing a special cryopreservative solution and porous beads, and were subcultured twice in enrichment media, i.e., Casein-peptone Soymeal-peptone (CASO) broth or MRS broth. CASO broth, a universal culture medium free from inhibitors, contained 17.0 g of pancreatic digest of casein, 3.0 g of papaic digest of soybean meal, 5.0 g of sodium chloride, 2.5 g of dipotassium hydrogen phosphate, 2.5 g of glucose, 1.0 g of skimmed milk powder, and 1000 mL of distilled water. The final pH of CASO broth was 7.3 ± 0.2 at 25 °C. Commercial MRS broth (Merck, Darmstadt, Germany) was rehydrated in distilled water according to manufacturer’s instructions, and hydrochloric acid (1 M HCl) was added to adjust the pH of the medium to 6.2 ± 0.2 at 25 °C. Both enrichment broths were sterilized by autoclaving at 121 °C for 15 min before use.
Streptococcus thermophilus TH-4 and S. thermophilus DSM 20479 were propagated in CASO broth at 37 °C for 48 h under aerobic conditions; Lc. lactis subsp. lactis ATCC 19435, Lc. lactis subsp. lactis biovar. diacetylactis VK-256, and Ln. mesenteroides subsp. dextranicum ATCC 19255 were grown in CASO broth at 30 °C for 72 h aerobically; whereas the rest of the strains tested in this study were incubated in MRS broth at 37 °C for 72 h in anaerobiosis. Anaerobic conditions were generated using anaerobic culture jars (2.5 L) and AnaeroGen AN 25 sachets (Oxoid, Basingstoke, United Kingdom). The pure culture suspensions thus prepared were used to test the performance of various culture media.
Süle J., Kõrösi T., Hucker A, & Varga L. (2014). Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria. Brazilian Journal of Microbiology, 45(3), 1023-1030.
Publication 2014

Most recents protocols related to «Casein peptone»

1
Microbial Growth and Motility Media
Casein Soya Bean Digest Agar (TSA): Tryptone or casein peptone pancreatic digest 17.0 g, soybean tryptone 3.0 g, NaCl 5.0 g, K2HPO4 2.5 g, gluconate 2.5 g, agar 20.0 g, distilled water 1,000 mL, PH7.3, sterilization at 121°C for 20 min, then pour into the culture dish.
Tryptone Soy Broth (TSB): Tryptone or casein peptone pancreatic digest 17.0 g, soybean tryptone 3.0 g, NaCl 5.0 g, K2HPO4 2.5 g, gluconate 2.5 g, distilled water 1,000 mL, PH7.3, sterilization at 121°C for 20 min.
Swimming medium: Beef extract 3.0 g, proteose peptone 10.0 g, NaCl 5.0 g, agar 3.0 g, distilled water 1,000 mL, pH 7.0 ~ 7.2, sterilized at 121°C for 20 min, poured into the medium.
Xie J., Yang J., Zhu S., Hou X., Chen H., Bai X, & Zhang Z. (2024). Study on seed-borne cultivable bacterial diversity and antibiotic resistance of Poa pratensis L.. Frontiers in Microbiology, 15, 1347760.
Full text: Click here
Publication 2024
2
Bacterial Strain Cultivation and Identification
Staphylococcus epidermidis (DSM-20044), Enterococcus faecium (DMS-2146) and Pseudomonas aeruginosa (DSM-939) were obtained from ATCC. Methicillin-resistant Staphylococcus aureus (MRSA) was a clinical isolate, kindly provided by Beniam Ghebremedhin (Helios University Hospital, Wuppertal, Germany). All strains were cultured on casein/soy peptone agar plates (CSA; pH 7.2) with 15 g/L casein peptone, 5 g/L soy peptone, 5 g/L sodium chloride and 15 g/L agar (AppliChem, Darmstadt, Germany) under aerobic conditions at 37 °C.
Dietrich M., Besser M, & Stuermer E.K. (2024). Characterization of the Human Plasma Biofilm Model (hpBIOM) to Identify Potential Therapeutic Targets for Wound Management of Chronic Infections. Microorganisms, 12(2), 269.
Full text: Click here
Publication 2024
3
Nitrogen Source Impact on Lactobacillus Growth
Not available on PMC !
To compare the growth ability of HY7720 in media containing nitrogen source derived from a plant or animal source, all tested media were prepared based on MRS medium with modifications. The specific recipe was as follows: glucose, 20 g/L; yeast extract, 15 g/L; complex nitrogen source, 10 g/L; dipotassium hydrogen phosphate, 2 g/L; sodium acetate, 5 g/L; diammonium hydrogen citrate, 2 g/L; magnesium sulfate, 0.01 g/L; manganese sulfate, 0.04 g/L; and polysorbate 80, 1 g/L. The following nitrogen sources were tested: soy peptone such as Hy-Soy™ Kosher IPS (Kerry Bio-Science, Norwich, NY, USA) and HSP-349 (The Tatua Co-Operative Dairy Co., Ltd., Morrinsville, New Zealand), Broadbean peptone (Solabia Group, Pantin, France), casein peptone such as Bacto™ Tryptone from casein (Beckton Dickinson, CA, USA) and HCP-321 (The Tatua Co-Operative Dairy Co., Ltd.), and lactoalbumin peptone (Tatua-2016, The Tatua Co-Operative Dairy Co., Ltd.). The growth curves were recorded at 37 °C by measuring absorbance at 600 nm (OD 600 ), and the maximum specific growth rate and the slope of the exponential growth phase (μ max = Δln(OD 600 )/Δt) were determined.
Kim J., Choi E., Jang W.Y., Kim S., Heo K., Kang H., Kang J., Kim Y., Shim J., Lee J., & Lee J.H. (2024). Evaluation of antioxidant activity and fermentation properties of potential probiotic strain Lactiplantibacillus plantarum HY7720 in plant-based materials. Deleted Journal, 6(5).
Publication 2024
4
Microbial Protease Production Protocol

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2024
5
Enzymatic Activities of Bacterial Isolates
The isolates were tested for various enzymatic activities, including catalase, oxidase, and urease. Substrate and hydrolysis tests were performed using media supplemented with specific substrates such as casein, starch, gelatin, and citrate [4 (link),11 (link),13 (link)].
Starch hydrolysis was tested on peptone yeast extract agar, replacing sucrose with 10 g of soluble starch per liter. A clear zone around the colony indicated a positive reaction after flooding with Gram’s iodine solution. Gelatin hydrolysis was tested on tubes containing a solid gelatin medium. A positive result was fluid production due to the bacterial enzyme gelatinase. Casein hydrolysis was tested on peptone yeast extract agar containing 10 g glucose per liter and 25 mL 15% (w/v) skim milk per liter and adjusted to pH 5.6. A positive result was a clear zone around a strip inoculation after flooding with 10% (w/v) HgCl2.
Aqel H., Farah H, & Al-Hunaiti A. (2024). Ecological versatility and biotechnological promise: Comprehensive characterization of the isolated thermophilic Bacillus strains. PLOS ONE, 19(4), e0297217.
Full text: Click here
Publication 2024

Top products related to «Casein peptone»

1
Yeast extract by Merck Group
Sourced in United States, Germany, United Kingdom, Spain, China, Australia, France, India, Switzerland, Italy, Poland, Sao Tome and Principe, Sweden
Yeast extract is a versatile laboratory product derived from the autolysis of baker's yeast. It contains a rich source of amino acids, vitamins, and other essential nutrients that are beneficial for the cultivation and growth of microorganisms. Yeast extract is commonly used as a nutrient supplement in microbial culture media for a variety of applications, including fermentation processes, cell culture, and the production of various biological products.
2
Casein peptone by Merck Group
Sourced in Germany, United States, United Kingdom
Casein peptone is a culture medium component derived from the enzymatic hydrolysis of casein. It provides a source of amino acids, peptides, and other nutrients to support the growth of various microorganisms in laboratory cultures.
3
Sodium chloride (nacl) by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, France, Spain, China, Canada, Sao Tome and Principe, Poland, Belgium, Australia, Switzerland, Macao, Denmark, Ireland, Brazil, Japan, Hungary, Sweden, Netherlands, Czechia, Portugal, Israel, Singapore, Norway, Cameroon, Malaysia, Greece, Austria, Chile, Indonesia
NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
4
Peptone from casein by Merck Group
Sourced in Germany
Peptone from casein is a laboratory-grade nutrient source derived from the enzymatic hydrolysis of casein, a milk-based protein. It provides a complex mixture of amino acids, peptides, and other nutrients that can support the growth and cultivation of various microorganisms in cell culture and microbiology applications.
5
Casein by Merck Group
Sourced in United States, Germany, Spain, Australia, United Kingdom, China
Casein is a type of milk protein that is commonly used as a laboratory reagent. It is a complex mixture of related phosphoproteins that are the main proteins in milk. Casein serves as a core function as a source of amino acids and other nutrients for cell growth and maintenance in various cell culture and biochemical applications.
6
Peptone by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, China, Hungary, Netherlands, Australia, Spain
Peptone is a laboratory-grade nutrient product manufactured by Merck Group. It is a complex mixture of peptides and amino acids derived from the enzymatic hydrolysis of proteins. Peptone serves as a source of nitrogen, carbon, and other essential nutrients to support the growth and cultivation of various microorganisms in microbiological media and cell culture applications.
7
Hydrochloric acid (hcl) by Merck Group
Sourced in Germany, United States, United Kingdom, India, Italy, France, Spain, Australia, China, Poland, Switzerland, Canada, Ireland, Japan, Singapore, Sao Tome and Principe, Malaysia, Brazil, Hungary, Chile, Belgium, Denmark, Macao, Mexico, Sweden, Indonesia, Romania, Czechia, Egypt, Austria, Portugal, Netherlands, Greece, Panama, Kenya, Finland, Israel, Hong Kong, New Zealand, Norway
Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
8
Starch by Merck Group
Sourced in United States, Germany, United Kingdom, France, India, Sao Tome and Principe, Italy, Ireland, Switzerland
Starch is a carbohydrate-based laboratory reagent used in various testing and analytical procedures. It serves as a general indicator for the presence of iodine, which forms a characteristic blue-black complex when combined with starch. The core function of starch is to provide a visual detection method for iodine, without further interpretation or extrapolation on its intended use.
9
Violet red bile agar by Merck Group
Sourced in Germany
Violet Red Bile Agar is a microbiological growth medium used for the isolation and enumeration of Enterobacteriaceae, particularly coliforms, in food and water samples. It is a selective and differential agar that suppresses the growth of Gram-positive bacteria while allowing the growth of Gram-negative bacteria.

More about "Casein peptone"

Casein peptone is a nutrient-rich complex of peptides derived from the enzymatic hydrolysis of casein, a major milk protein.
It serves as a versatile growth medium component, supporting the cultivation of a wide range of microorganisms in laboratory settings.
Casein peptone provides a rich source of amino acids, peptides, and other essential nutrients required for microbial proliferation and metabolism.
Researchers can leverage the power of PubCompare.ai's AI-driven platform to enhance the reproducibility and accuracy of their casein peptone research.
This tool helps locate relevant protocols from literature, preprints, and patents, while enabling intelligent comparisons to identify the most optimal protocols and products.
By utilizing PubCompare.ai, researchers can improve the quality and efficiency of their casein peptone studies, leading to better overall outcomes.
Casein peptone is closely related to other important compounds like yeast extract, NaCl, peptone from casein, casein, peptone, hydrochloric acid, starch, and Violet Red Bile Agar.
These substances play crucial roles in microbial growth, nutrient provision, and laboratory techniques.
Researchers should familiarize themselves with the synergies and interactions between these materials to optimize their casein peptone experiments.
With the help of PubCompare.ai's advanced features, scientists can streamline their casein peptone research, ensuring reproducibility, accuracy, and efficient protocol identification.
This can lead to breakthroughs in various fields, from microbiology to biotechnology, where casein peptone is a vital component.