Key inclusion criteria were: (a) presence of PDD (autism, PDD-NOS, Asperger’s disorder) established by DSM-IV-TR clinical criteria1 and corroborated by the Autism Diagnostic Interview-Revised (ADI-R);36 (link) (b) age 4 to 13 years inclusive; (c) ≥ 18 on the Irritability subscale of the parent-rated ABC; 37 (link),38 (link) (d) CGI—Severity score ≥ 4; (e) medication free for two weeks for most psychotropic drugs, and for four weeks for fluoxetine and/or depot neuroleptics; (f) IQ ≥ 35 or mental age ≥ 18 months as assessed by Stanford Binet, Leiter International Performance Scale, or Mullen Scales of Development; and (g) if taking anticonvulsant, seizure-free for ≥ six months and with stable dose for four weeks. Exclusion criteria included (a) positive Beta HCG pregnancy test for girls; (b) prior adequate trial of risperidone; (c) other PDD (i.e., Rett’s disorder, Childhood Disintegrative Disorder); (d) lifetime diagnosis of schizophrenia, other psychotic disorder, bipolar disorder, or current diagnosis of major depression, obsessive compulsive disorder, or substance abuse; (e) significant medical condition (e.g., heart, liver, renal, pulmonary disease), unstable seizure disorder, or significant abnormality on routine laboratory tests.12 (link)
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Chorionic Gonadotropin, beta Subunit, Human
Chorionic Gonadotropin, beta Subunit, Human
Chorionic Gonadotropin, beta Subunit, Human: A glycoprotein hormone produced by the placenta during pregnancy.
The beta subunit is unique to this hormone and is essential for its biological activity.
It plays a crucial role in the maintenance of the corpus luteum and stimulation of steroidogenesis during early pregnancy.
Measurement of this hormone is used for pregnancy testing and monitoring trophoblastic disease.
The beta subunit is unique to this hormone and is essential for its biological activity.
It plays a crucial role in the maintenance of the corpus luteum and stimulation of steroidogenesis during early pregnancy.
Measurement of this hormone is used for pregnancy testing and monitoring trophoblastic disease.
Most cited protocols related to «Chorionic Gonadotropin, beta Subunit, Human»
Anticonvulsants
Antipsychotic Agents
Asperger Syndrome
Autistic Disorder
Bipolar Disorder
Chorionic Gonadotropin, beta Subunit, Human
Diagnosis
Epilepsy
Fluoxetine
Heart
Inclusion Bodies
Kidney
Liver
Lung Diseases
Major Depressive Disorder
Obsessive-Compulsive Disorder
Parent
Pharmaceutical Preparations
Pregnancy Tests
Psychotic Disorders
Psychotropic Drugs
Rett Syndrome
Risperidone
Schizophrenia
Seizures
Substance Abuse
Verbascum
Woman
Study sessions were scheduled between 3 and 30 days apart. Participants typically arrived at the day hospital unit at 11:00 am on the day of testing after abstaining from alcohol for 48 hours. A breathalyzer test was performed to ensure a zero BrAC. A urine sample was collected for a urine drug screen for all participants and a urine beta-hCG test for females; both were required to be negative to continue participation in the study. Participants received a light snack and completed brief medical and drinking history questionnaires. An indwelling i.v. catheter was then inserted into a vein in the antecubital fossa of (preferably) the nondominant arm using sterile technique. This catheter was used for the alcohol infusion.
The participant was seated in a comfortable chair in a study room in the day hospital unit, out of sight of the infusion pumps and technician’s screen, then instructed in the procedures and limits for selecting alcohol self-infusions in the paradigm. Participants were told to administer alcohol as if they were in a social situation in which they usually drink alcohol. To control the ambient environment during the self-administration session, participants were allowed to watch television or listen to music. The experimenter was available to monitor the infusion and obtain breathalyzer readings as well as to answer any questions raised by the participants and to occasionally inquire about the well-being of the participant.
Subjective measures were obtained serially to assess alcohol effects. These measures were collected at baseline as well as during the directed priming phase (at the 10- and 20-minute time points) and 8 times during the IV-ASA session every 15 minutes, with a final postinfusion measure 15 minutes after the IV-ASA had ended. These measures took roughly 5 minutes to complete. Subjects were allowed to press for more alcohol during data collection so as to not interfere with the subject’s opportunity to self-administer alcohol. These included the Alcohol Urge Questionnaire (AUQ) (Bohn et al., 1995 (link)) and Drug Effects Questionnaire (DEQ) (Fischman and Foltin, 1991 (link)).
At the end of the free-access phase, the infusion pump was disconnected, and the i.v. catheter was removed from the participant’s arm. Lunch was provided and serial breathalyzer tests tracked the BrAC. Participants were asked to stay in the hospital for at least 2 hours after the end of the self-administration or until their BrAC level fell <20 mg%, whichever was later. At this time, participants were debriefed and sent home in a taxi paid for by NIH. The total duration of the session was approximately 7 hours. Study participants were instructed to refrain from medications and operating any machinery requiring concentration for at least 2 hours following their release from the unit.
The participant was seated in a comfortable chair in a study room in the day hospital unit, out of sight of the infusion pumps and technician’s screen, then instructed in the procedures and limits for selecting alcohol self-infusions in the paradigm. Participants were told to administer alcohol as if they were in a social situation in which they usually drink alcohol. To control the ambient environment during the self-administration session, participants were allowed to watch television or listen to music. The experimenter was available to monitor the infusion and obtain breathalyzer readings as well as to answer any questions raised by the participants and to occasionally inquire about the well-being of the participant.
Subjective measures were obtained serially to assess alcohol effects. These measures were collected at baseline as well as during the directed priming phase (at the 10- and 20-minute time points) and 8 times during the IV-ASA session every 15 minutes, with a final postinfusion measure 15 minutes after the IV-ASA had ended. These measures took roughly 5 minutes to complete. Subjects were allowed to press for more alcohol during data collection so as to not interfere with the subject’s opportunity to self-administer alcohol. These included the Alcohol Urge Questionnaire (AUQ) (Bohn et al., 1995 (link)) and Drug Effects Questionnaire (DEQ) (Fischman and Foltin, 1991 (link)).
At the end of the free-access phase, the infusion pump was disconnected, and the i.v. catheter was removed from the participant’s arm. Lunch was provided and serial breathalyzer tests tracked the BrAC. Participants were asked to stay in the hospital for at least 2 hours after the end of the self-administration or until their BrAC level fell <20 mg%, whichever was later. At this time, participants were debriefed and sent home in a taxi paid for by NIH. The total duration of the session was approximately 7 hours. Study participants were instructed to refrain from medications and operating any machinery requiring concentration for at least 2 hours following their release from the unit.
Auditory Perception
Breathalyzer Tests
Catheters
Chorionic Gonadotropin, beta Subunit, Human
Ethanol
Females
Indwelling Catheter
Infusion Pump
Light
Pharmaceutical Preparations
Self Administration
Snacks
Sterility, Reproductive
Substance Abuse Detection
Urinalysis
Urine
Veins
Vision
Adolescent
Anticonvulsants
Asperger Syndrome
Autistic Disorder
Bipolar Disorder
Child
Chorionic Gonadotropin, beta Subunit, Human
Diagnosis
Fluoxetine
Gender
Major Depressive Disorder
Obsessive-Compulsive Disorder
Parent
Pervasive Development Disorders
Pharmaceutical Preparations
Pregnancy Tests
Problem Behavior
Psychotic Disorders
Psychotropic Drugs
Seizures
Substance Abuse
Verbascum
Woman
Antibodies
Antigens
Buffers
Chorionic Gonadotropin, beta Subunit, Human
Cloning Vectors
Edetic Acid
Hematoxylin
Immunoglobulins
Mice, House
Peroxidases
Peroxide, Hydrogen
Polymers
Rabbits
Tissues
Tromethamine
hCG was analyzed in serum using a solid-phase two-site chemiluminescent immunometric assay, calibrated against WHO 3rd IS 75/537, on an Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA). The Siemens assay detects serum intact hCG, hyperglycosylated hCG, serum nicked hCG, serum nicked hyperglycosylated hCG, serum asialo hCG, serum hCG free β-subunit and serum nicked hCG β [25 (link)]. The inter assay coefficient of variation was 8.0, 6.3 and 5.1 % at the concentration of 9.7, 53.1 and 821.5 IU/L, respectively. Although the Immulite 2000 is considered as one of the best assays for total hCG, it should be noted that the reference ranges in this paper are assay specific and do not correspond with hCG values obtained from different assays [26 ].
asialo-human chorionic gonadotropin
Biological Assay
Chemiluminescent Assays
Chorionic Gonadotropin, beta Subunit, Human
Diagnosis
Serum
Most recents protocols related to «Chorionic Gonadotropin, beta Subunit, Human»
Research nurses collected data at 3-monthly study visits using interviewer-administered questionnaires. Data were collected on sociodemographic characteristics, sexual behavior, contraceptive use, pregnancy, sexually transmitted infections (STIs), and substance use. Volunteers who became pregnant were linked to antenatal care services and followed up for a pregnancy outcome.
Laboratory tests included pregnancy tests for beta-human chorionic gonadotrophin (β-hcg) hormone performed on urine samples using QuickVue (Quidel Corporation, San Diego, CA, United States) and HIV tests following the national testing algorithm, which involves a screening test (Alere Medical Co. Ltd., Chuba, Japan), the StatPak rapid confirmatory kit (Chembo Diagnostics System Inc., Medford, NY, United States), and SD Bioline as a tiebreaker (Standard Diagnostics, Inc., South Korea). STIs (Neisseria gonorrhoeae and Chlamydia trachomatis) were tested on endocervical swabs using the GeneXpert platform (Cepheid AB, Rontgenvagen 5, Soina, Sweden).
Laboratory tests included pregnancy tests for beta-human chorionic gonadotrophin (β-hcg) hormone performed on urine samples using QuickVue (Quidel Corporation, San Diego, CA, United States) and HIV tests following the national testing algorithm, which involves a screening test (Alere Medical Co. Ltd., Chuba, Japan), the StatPak rapid confirmatory kit (Chembo Diagnostics System Inc., Medford, NY, United States), and SD Bioline as a tiebreaker (Standard Diagnostics, Inc., South Korea). STIs (Neisseria gonorrhoeae and Chlamydia trachomatis) were tested on endocervical swabs using the GeneXpert platform (Cepheid AB, Rontgenvagen 5, Soina, Sweden).
Care, Prenatal
Chlamydia trachomatis
Chorionic Gonadotropin, beta Subunit, Human
Contraceptive Agents
Diagnosis
Endocervix
Homo sapiens
Hormones
Interviewers
Neisseria gonorrhoeae
Nurses
Pregnancy
Pregnancy Tests
Sexually Transmitted Diseases
Substance Use
Testing, AIDS
Urine
Voluntary Workers
The NT is routinely measured in gestational week 11 to 13 + 6, as part of the first trimester prenatal-screening. The Danish sonographers adhere to the protocol of the Fetal Medicine Foundation (3 ) for scanning the NT. The first-trimester prenatal screening for syndromes and congenital anomalies include; Double-test with blood tests for PAPP-A and beta-hCG in gestational week 8–14, NT-measurement in gestational week 11–14. A risk-score is calculated based on the values from the double-test and the nuchal translucency and the maternal age. If the risk is above 1:300 for trisomy 21 and above 1:150 for trisomy 18 and 13, further diagnostics are offered. These further diagnostics include chorionic villus sampling with chromosomal microarray/array-CGH or amniocentesis. Non-invasive prenatal testing can be offered as an alternative to the further diagnostics, but this is not implemented as a routine or stand-alone tool by the Danish Fetal Medicine Society. In second trimester, gestational week 20–22, pregnant women are also offered a free fetal ultrasound scan to detect any fetal malformations. Prenatal screening is offered to all pregnant women. The screening is free-of-charge as part of access to tax-funded public free healthcare and >90% of all pregnant women attend this.
The NT was divided into NT < 95th centile or NT ≥ 95th centile. This cut-off was chosen as the 95th centile denotes an “increased” nuchal translucency, and a NT above the 95th warrants further prenatal testing. The 99th centile (3.5 mm) was included in the NT ≥ 95th centile, as this contained too few patients to analyze. The NT centiles were calculated based on the crown-rump-length (CRL) at the first trimester scan using the method and model as by the Fetal Medicine Foundation (39 (link)).
The NT was divided into NT < 95th centile or NT ≥ 95th centile. This cut-off was chosen as the 95th centile denotes an “increased” nuchal translucency, and a NT above the 95th warrants further prenatal testing. The 99th centile (3.5 mm) was included in the NT ≥ 95th centile, as this contained too few patients to analyze. The NT centiles were calculated based on the crown-rump-length (CRL) at the first trimester scan using the method and model as by the Fetal Medicine Foundation (39 (link)).
Amniocentesis
Chorionic Gonadotropin, beta Subunit, Human
Chromosomes
Congenital Abnormality
Diagnosis
Down Syndrome
Fetal Malformations
Fetal Ultrasonography
Hematologic Tests
Microarray Analysis
Nuchal Translucency
Patients
Pregnancy
Pregnancy-Associated Plasma Protein-A
Pregnant Women
Radionuclide Imaging
Trisomy 18
The primary outcome was clinical pregnancy rate, which was defined as the detection of fetal heart beats using ultrasonographic examinations 35 days after FET. Serum beta subunit of human chorionic gonadotropin (β-hCG) levels were examined using electrochemiluminescence analysis (Cobas e411 System Product, Germany) on day 14 following embryo transfer in all patients. Implantation was defined as a serum quantitative β-hCG ≥ 10 U/L. All pregnancies were followed until 7 weeks after FET. A biochemical loss is defined as a transient but significant increase in serum β-hCG ≥ 10 U/L between days 12 and 20 after embryo transfer without detecting a gestational sac by an ultrasonography. An early abortion is defined as the detection of an empty gestational sac using ultrasonographic examinations before 12 weeks of gestation. Implantation rate is defined as the ration of the number of embryos implanted and the number of transferred embryos.
Chorionic Gonadotropin, beta Subunit, Human
Embryo
Fetal Heart
Gestational Sac
Induced Abortions
Ovum Implantation
Patients
Physical Examination
Pregnancy
Serum
Transients
Ultrasonography
In all patients we performed ICSI fertilization alone, or there were several cases where we combined ICSI with the conventional IVF method. The indications for ICSI are mainly semen and sperm-cell abnormalities, advanced maternal age (above 35 years of age) or more than two unsuccessful previously performed conventional IVF treatments. The denuded oocytes before ICSI were placed in G-MOPSTM media (VitrolifeVR, Goteborg, Sweden), and assessed for maturity. For the following breeding, the fertilized oocytes were put in G1TMPLUS or G-TLTM single-step breeding media (VitrolifeVR, Goteborg, Sweden). The conventional IVF method was performed in G-IVFTM PLUS media (VitrolifeVR, Goteborg, Sweden), and 24 h later the fertilization was assessed (presence of two pronuclei cells), and these cells were also placed into the previously mentioned G1TMPLUS or G-TLTM media.
Only Grade 1 staged embryos were allowed to transfer (based on the ESHRE’s Consensus embryo scoring system) 3–5 days after the oocyte retrieval procedure in the cleavage or blastocyst embryo division stage. Embryo transfers (ET) were always controlled by transabdominal ultrasound. One, two or three embryos could be placed in the uterine cavity according to the patient’s wish. The success of the therapy was checked with serum human chorionic gonadotropin beta (beta-hCG) levels 14 days after ET. At this time, we checked for the presence of a biochemical pregnancy, as it is the first-line success indicator of our study.
Only Grade 1 staged embryos were allowed to transfer (based on the ESHRE’s Consensus embryo scoring system) 3–5 days after the oocyte retrieval procedure in the cleavage or blastocyst embryo division stage. Embryo transfers (ET) were always controlled by transabdominal ultrasound. One, two or three embryos could be placed in the uterine cavity according to the patient’s wish. The success of the therapy was checked with serum human chorionic gonadotropin beta (beta-hCG) levels 14 days after ET. At this time, we checked for the presence of a biochemical pregnancy, as it is the first-line success indicator of our study.
Blastocyst
Cells
Chorionic Gonadotropin, beta Subunit, Human
Congenital Abnormality
Cytokinesis
Dental Caries
Embryo
Fertilization
Homo sapiens
Oocyte Retrieval
Ovum
Patients
Plant Embryos
Pregnancy
Serum
Sperm
Sperm Injections, Intracytoplasmic
Therapeutics
Transfers, Embryo
Ultrasonography
Uterus
FTS was performed to determine the pregnancy-associated plasma protein-A (PAPP-A) and free beta subunit of human chorionic gonadotropin (β-hCG) levels at 9–13+6 weeks gestation and/or ultrasound fetal nuchal thickness (NT) at 11–13+6 gestation. Patients who had undergone first-trimester screening, but had not been screened in second-trimester screening are referred to as individual first-trimester screening (IFTS). Patients who had undergone first-trimester screening and had undergone second-trimester screening, then participated in the joint screening, are referred to as combined first-trimester screening (CFTS). STS was performed to determine the maternal serum alpha-fetoprotein (AFP) and free β-hCG levels at 15–20+6 weeks gestation. Patients who did not participate in first-trimester screening and could not participate in the joint screening in the later stage are referred to as individual second-trimester screening (ISTS). Those who participated in first-trimester screening, followed by second-trimester screening, and participated in joint screening are referred to as combined second trimester screening (CSTS), as shown in Fig 1 . FSTCS involved a triple- or quadruple-screening protocol with determination of AFP and free β-hCG levels in the second trimester and matching PAPP-A and/or NT outcomes in the first trimester. The specific FSTCS methodology involved reporting the high risk, but not low risk FTS results, awaiting the STS results, then combining the FTS results to evaluate the probability of a fetus with trisomy 21 or 18.
Chorionic Gonadotropin, beta Subunit, Human
Down Syndrome
Fetal Ultrasonography
Fetus
Joints
Patients
Pregnancy
Pregnancy-Associated Plasma Protein-A
Serum
Serum Proteins
Top products related to «Chorionic Gonadotropin, beta Subunit, Human»
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QuickVue is a rapid diagnostic test kit designed for the detection of various analytes. It utilizes a lateral flow immunoassay technology to provide quick and accurate results.
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The Immulite 2000 XPi system is an automated immunoassay analyzer designed for clinical laboratory use. It is capable of performing a variety of immunoassay tests to aid in the diagnosis and monitoring of various medical conditions.
The Rabbit anti-α HCG Antibody is a laboratory reagent used for the detection and quantification of the alpha subunit of human chorionic gonadotropin (α-HCG) in various biological samples. This antibody is produced by immunizing rabbits with the α-HCG antigen.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Ab131170 is a rabbit monoclonal antibody. It is designed for use in various immunoassay applications.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
More about "Chorionic Gonadotropin, beta Subunit, Human"
Chorionic Gonadotropin, beta Subunit, Human is a glycoprotein hormone produced by the placenta during pregnancy.
The beta subunit is unique to this hormone and is essential for its biological activity.
It plays a crucial role in the maintenance of the corpus luteum and stimulation of steroidogenesis during early pregnancy.
Measurement of this hormone is used for pregnancy testing and monitoring trophoblastic disease.
The human chorionic gonadotropin (hCG) beta subunit is a key marker for various clinical applications, including pregnancy testing, monitoring of pregnancy progression, and detection of gestational trophoblastic disease.
The QuickVue and Immulite 2000 XPi system are examples of diagnostic assays that can be used to measure hCG beta subunit levels.
Rabbit anti-α HCG Antibody is a specific antibody that can be used to detect the alpha subunit of hCG, which is shared with other glycoprotein hormones.
DMSO and AutoDELFIA are techniques that may be employed in the analysis of hCG beta subunit samples.
Researchers may also utilize the Ab131170 antibody or U-Plex assay to study the hCG beta subunit.
Penicillin and Streptomycin are commonly used antibiotics that may be incorporated into cell culture media when working with hCG-producing cells.
The Infinite M Nano is an example of a microplate reader that can be used to quantify hCG beta subunit levels in biological samples.
The beta subunit is unique to this hormone and is essential for its biological activity.
It plays a crucial role in the maintenance of the corpus luteum and stimulation of steroidogenesis during early pregnancy.
Measurement of this hormone is used for pregnancy testing and monitoring trophoblastic disease.
The human chorionic gonadotropin (hCG) beta subunit is a key marker for various clinical applications, including pregnancy testing, monitoring of pregnancy progression, and detection of gestational trophoblastic disease.
The QuickVue and Immulite 2000 XPi system are examples of diagnostic assays that can be used to measure hCG beta subunit levels.
Rabbit anti-α HCG Antibody is a specific antibody that can be used to detect the alpha subunit of hCG, which is shared with other glycoprotein hormones.
DMSO and AutoDELFIA are techniques that may be employed in the analysis of hCG beta subunit samples.
Researchers may also utilize the Ab131170 antibody or U-Plex assay to study the hCG beta subunit.
Penicillin and Streptomycin are commonly used antibiotics that may be incorporated into cell culture media when working with hCG-producing cells.
The Infinite M Nano is an example of a microplate reader that can be used to quantify hCG beta subunit levels in biological samples.