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Ciliary Neurotrophic Factor

Ciliary Neurotrophic Factor (CNTF) is a protein that plays a crucial role in the survival and differentiation of various neuronal cell types, including motor neurons, sensory neurons, and retinal ganglion cells.
It is a member of the interleukin-6 cytokine family and has been implicated in the treatment of neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) and Alzheight's disease.
CNTF has also been studied for its potential to promote nerve regeneration and neuroprotection.
Researchers continue to investigate the therapeutic applications of CNTF and optimize research protocols to ensure reproducibility and accuracy in their studies.

Most cited protocols related to «Ciliary Neurotrophic Factor»

Differentiation of hPSCs into Neurons

Cited 36 times

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Publication 2018

4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide accutase Adrenal Cortex Cells Ciliary Neurotrophic Factor Doxycycline Hyclate Glial Cell Line-Derived Neurotrophic Factor LDN 193189 Mus Neuroglia Neurotrophic Factor, Brain-Derived Puromycin Tetracycline Trans-Activators XAV939 Y 27632

Differentiation of Motor Neurons from hPSCs

Cited 19 times

To generate MNPs, hPSCs were dissociated with Dispase (1 mg/ml) and split 1:6 on irradiated MEFs or Matrigel coated plates. On the following day, the PSC medium was replaced with a chemically defined neural medium, including DMEM/F12, Neurobasal medium at 1:1, 0.5×N2, 0.5×B27, 0.1mM ascorbic acid (Santa Cruz), 1×Glutamax and 1×penicillin/streptomycin (All others from Invitrogen). CHIR99021 (3uM, Torcris), 2μM DMH-1 (Torcris) and 2μM SB431542 (Stemgent) were added in the medium. The culture medium was changed every other day. Human PSCs maintained under this condition for 6 days were induced into NEP cells. The NEP cells were then dissociated with Dispase (1 mg/ml) and split at 1:6 with the same medium described above. RA (0.1μM, Stemgent) and 0.5μM Purmorphamine (Stemgent) were added in combination with 1μM CHIR99021, 2μM DMH-1, and 2μM SB431542. The medium was changed every other day. NEP cells maintained under this condition for 6 days differentiated into OLIG2+ MNPs. The OLIG2⁺ MNPs were expanded with the same medium containing 3μM CHIR99021, 2μM DMH-1, 2μM SB431542, 0.1μM RA, 0.5μM Purmorphamine and 0.5 mM VPA (Stemgent), and split 1:6 once a week with Dispase (1 mg/ml). OLIG2⁺ MNPs were frozen with the regular frozen medium (DMEM/F12, 10% fetal bovine serum and 10% DMSO) in liquid nitrogen, and cultured again in expansion medium after thawing.
To induce MN differentiation, OLIG2⁺ MNPs were dissociated with Dispase (1 mg/ml) and cultured in suspension in the above neural medium with 0.5μM RA and 0.1μM Purmorphamine. The medium was changed every other day. OLIG2⁺ MNPs under this condition for 6 days differentiated into MNX1⁺ MNs. The MNX1⁺ MNs were then dissociated with Accumax (eBioscience) into single cells and plated on Matrigel coated plates or on astrocytes. The MNX1⁺ MNs were cultured with 0.5μM RA, 0.1μM Purmorphamine and 0.1μM Compound E (Calbiochem) for 10 days to mature into CHAT⁺ MNs. Insulin-like growth factor 1(IGF-1), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) (all from R&D, 10 ng/ml each) were added if MNs were plated at low density. For identifying MN disease phenotypes, SMA and ALS MNs were cultured without these neurotrophic factors.

Du Z.W., Chen H., Liu H., Lu J., Qian K., Huang C.T., Zhong X., Fan F, & Zhang S.C. (2015). Generation and Expansion of highly-pure Motor Neuron Progenitors from Human Pluripotent Stem Cells. Nature communications, 6, 6626.

Publication 2015

4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide Ascorbic Acid Astrocytes Cells Chir 99021 Ciliary Neurotrophic Factor Cortisone dispase Fetal Bovine Serum Freezing Homo sapiens IGF1 protein, human matrigel Nerve Growth Factors Nervousness Neurotrophic Factor, Brain-Derived Nitrogen OLIG2 protein, human Pancreatic Stellate Cells Penicillins Phenotype purmorphamine Streptomycin Sulfoxide, Dimethyl

Motor Neuron Differentiation and Maturation Protocol

Cited 16 times

Motor neuron differentiation was performed as described before, with some modifications^{32 (link)}. iPSC clones were treated with collagenase type IV to form small clusters and resuspended in Essential^TM 8 medium. During the first 2 days, medium was changed every day with Neuronal basic medium (DMEM/F12 plus Neurobasal medium with N2 and B27 supplement without vitamin A) supplemented with 40 μM SB431542 (Tocris Bioscience), 0.2 μM LDN-193189 (Stemgent), 3 µM CHIR99021 (Tocris Bioscience), and 5 µM Y-27632 (Merck Millipore). From day 3 on, 0.1 µM retinoic acid (Sigma) and 500 nM SAG (Merck Millipore) was added. From day 8 on, BDNF (10 ng/ml, Peprotech) and GDNF (10 ng/ml, Peprotech) were added. DAPT (20 µM, Tocris Bioscience) was added on day 9. Floating clusters were dissociated into single cells for plating on day 11 by using 0.05% trypsin (Gibco^TM). Motor neuron progenitors were subsequently plated on laminin (20 μg/ml)-coated 12-well plates at 0.5–2 × 10⁵ cells per well. From day 17 on, the cells were switched to motor neuron maturation medium supplemented with BDNF, GDNF, and CNTF (each 10 ng/ml, Peprotech) to keep long term cultures. Media were changed every other day by replacing half of the medium. For rescue experiments, motor neurons were treated overnight with either 1 µM Tubastatin A (Sigma), 1 µM ACY-738 (Acetylon Pharmaceuticals Inc., Boston, USA) or an equivalent amount of DMSO.

Guo W., Naujock M., Fumagalli L., Vandoorne T., Baatsen P., Boon R., Ordovás L., Patel A., Welters M., Vanwelden T., Geens N., Tricot T., Benoy V., Steyaert J., Lefebvre-Omar C., Boesmans W., Jarpe M., Sterneckert J., Wegner F., Petri S., Bohl D., Vanden Berghe P., Robberecht W., Van Damme P., Verfaillie C, & Van Den Bosch L. (2017). HDAC6 inhibition reverses axonal transport defects in motor neurons derived from FUS-ALS patients. Nature Communications, 8, 861.

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Publication 2017

1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide ACY-738 Cells Chir 99021 Ciliary Neurotrophic Factor Clone Cells GDNF protein, human Induced Pluripotent Stem Cells Laminin LDN 193189 Matrix Metalloproteinase 2 Motor Neurons Neurons Pharmaceutical Preparations Sulfoxide, Dimethyl Tretinoin Trypsin Vitamin A Y 27632

Differentiation of Motor Neurons from hPSCs

Cited 16 times

Publication 2015

Optimized Oligodendrocyte Progenitor Isolation

Cited 12 times

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Publication 2012

2-Mercaptoethanol Bicarbonate, Sodium Cells Ciliary Neurotrophic Factor Deoxyribonuclease I Edetic Acid Fibroblast Growth Factor 2 Glucose HEPES Insulin isolation Nerve Tissue Papain PDGF AA Penicillins Phosphates Powder Saline Solution Serum Albumin, Bovine Streptomycin Thyronine Tissues Trypsin

Most recents protocols related to «Ciliary Neurotrophic Factor»

Western Blot Analysis of Lumbar DRG and Sciatic Nerve

The dissected lumbar 4 and 5 (L4 and L5) DRG and sciatic nerve tissue were rinsed with phosphate-buffered saline and lysed in Triton lysis buffer. Being denatured proteins were separated on sodium dodecyl sulphate-polyacrylamide gel and then transferred onto polyvinylidene difluoride membrane on ice at 200 mA for 2 hr. The membranes were blocked with 5% skim milk, 0.1% Tween 20 in tris buffered saline for 30 min at room temperature. Then, the membranes were incubated overnight with primary antibodies at 4ºC. Protein (20 μg) was used for Western blot analysis using anti-TrkB rabbit polyclonal antibody (1:1,000, Cell Signaling Biotechnology, Danvers, MA, USA), anti-β-actin mouse monoclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-NF-κB mouse monoclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-CNTF mouse monoclonal antibody (1:1,000, Cell Signaling Biotechnology), anti-NGF mouse monoclonal antibody (1:1,000, Cell Signaling Biotechnology), anti-BDNF mouse monoclonal antibodies (1:1,000, Santa Cruz Biotechnology), anti-TNF-α rabbit polyclonal antibody (1:1,000, Sino Biological, Wayne, PA, USA), anti-IL-6 rabbit polyclonal antibody (1:1,000, GeneTex Inc., Irvine, CA, USA). For the secondary antibody, Horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (1:1,000, GeneTex Inc.) were used. The blotting proteins were detected by using Westar ECL substrates (Cyanagen, Bologna, Italy) and detected band intensity was analyzed using Chemidoc (Bio-Rad, Hercules, CA, USA).

Yu J.I., Cho Y.H., Seo T.B, & Kim Y.P. (2023). Effect of combined intervention of exercise and autologous bone marrow stromal cell transplantation on neurotrophic factors and pain-related cascades over time after sciatic nerve injury. Journal of Exercise Rehabilitation, 19(1), 19-26.

Publication 2023

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Differentiation of iNIL Stem Cells into Motor Neurons

iNIL stem cells were generously provided by Dr Esteban Mazzoni (New York University) and maintained in the 2i‐based media (Table S3) containing leukemia inhibitory factor (ESG1106; Millipore), CHIR99021 (NC0664823; Fisher Scientific, Hanover Park, IL, USA), and PD0325901 (NC0759248; Fisher Scientific). To differentiate iNIL cells into motor neurons, cells were lifted using TrypLE (12‐604‐021; Fisher) and feeder media (Table S4), seeded to a suspension culture dish (08‐772‐32; Fisher) in AK media (Table S5) [37 ], and incubated for 2 days to allow the embryoid body formation. Doxycycline was added to the suspension culture to induce the expression of three transcription factors, neurogenin‐2, islet‐1, and lhx‐3. These three transcription factors drive neuronal specification in the embryoid body. Two days later, cells were dissociated from the embryoid body using a standard approach, and the dissociated cells were resuspended in the motor neuron media cocktail containing neurotrophic factors (GDNF, BDNF, and CNTF; Table S6). The resuspended cells were plated on poly‐l‐ornithine (PLO)‐coated plates and incubated for 2 days for motor neuron maturation. The drugs were treated to the motor neuron culture at this stage. A more detailed protocol with media recipe for creating motor neurons from iNIL stem cell was described previously [32 (link)]. The file Appendix S1 contains tables that show all culture reagents with working concentrations.

Martinez A.M., Kim A., Flores C.A., Rahman D.F, & Yang W.S. (2023). Mouse embryonic stem cell‐derived motor neurons are susceptible to ferroptosis. FEBS Open Bio, 13(3), 419-433.

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Publication 2023

Cells Chir 99021 Ciliary Neurotrophic Factor Doxycycline Embryoid Bodies Glial Cell Line-Derived Neurotrophic Factor Hyperostosis, Diffuse Idiopathic Skeletal LIF protein, human Motor Neurons Nerve Growth Factors Neurons PD-0325901 Pharmaceutical Preparations polyornithine Stem Cells Transcription Factor

Isolation and Differentiation of Fetal Neural Stem Cells

All protocols described herein were carried out according to the European Community Council Directives (86/609/EEC) and comply with the guidelines published in the NIH Guide for the Care and Use of Laboratory Animals.
Fetal NSCs were isolated from E.13.5 forebrain as already described in a detailed methodological publication, including the culture characterization, the OGD, and the high content screening-based analysis of cell death and differentiation (Baldassarro, 2021 (link); Figure 1A). In brief, tissues were incubated in non-enzymatic dissociation buffer (Sigma-Aldrich, Saint Louis, MO, USA) at 37°C for 15 min, then mechanically dissociated by pipetting. Cells were resuspended in serum-free medium (DMEM/F12 GlutaMAX; 8 mmol/L HEPES; 100 U/100 μg Penicillin/Streptomycin; 1 × B27; 1 × N2; 20 ng/mL bFGF; 20 ng/mL EGF; Thermo Fisher Scientific, Waltham, MA, USA) and plated in suspension, at a density of 10 cells/μl in flasks (Nunc, Roskilde, DK) kept in vertical to avoid cell adhesion. Half medium was changed every 3 days, centrifuging the cell suspension at 300 × g for 5 min and gently resuspending the cellular pellet in fresh medium. Neurospheres were allowed to proliferate until they attained a diameter of about 100 μm.
To obtain the oligospheres, primary neurospheres were centrifuged at 300 × g for 5 min. The pellet was mechanically dissociated by pipetting, and cells were counted and plated again at a density of 10 cells/μl in OPC medium (DMEM/F12 GlutaMAX; 8 mmol/L HEPES; 100 U/100 μg Penicillin/Streptomycin; 1 × B27; 1 × N2; 20 ng/mL bFGF; 20 ng/mL PDGF; Thermo Fisher Scientific, Waltham, MA, USA). The oligospheres were centrifuged and the pellet mechanically dissociated to obtain a single cell suspension. Following cell count, cells were plated at a density of 3,000 cells/cm² on poly-D,L-ornithine (50 μg/ml)/laminin (5 μg/ml; Sigma-Aldrich) coating, in OPC medium.
To induce oligodendrocyte differentiation and maturation, the OPC medium was replaced with the oligodendrocyte differentiation medium (DMEM/F12 GlutaMAX; 8 mmol/L HEPES; 100 U/100 μg Penicillin/Streptomycin; 1 × B27; 1 × N2; 50 nM T3; 10 ng/ml CNTF; 1 × N-acetyl-L-cysteine – NAC; Thermo Fisher Scientific, Waltham, MA, USA) following 3 DIVs.
To characterize the responsiveness of OPCs to NGF in the mixed cultures, in a set of experiments we treated cultures with vehicle or NGF (100 ng/ml) at DIV 0 for 24 h (Figure 2A).

Baldassarro V.A., Cescatti M., Rocco M.L., Aloe L., Lorenzini L., Giardino L, & Calzà L. (2023). Nerve growth factor promotes differentiation and protects the oligodendrocyte precursor cells from in vitro hypoxia/ischemia. Frontiers in Neuroscience, 17, 1111170.

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Publication 2023

Acetylcysteine Animals, Laboratory Buffers Cell Adhesion Cells Ciliary Neurotrophic Factor Enzymes Fetus HEPES Laminin Oligodendroglia Ornithine Penicillins Platelet-Derived Growth Factor Poly A Prosencephalon Serum Streptomycin Tissues

Differentiation of Human iPSCs into Motor Neurons

Human iPSCs were routinely cultured in feeder-free conditions using Matrigel™-coated plates with mTeSR1 pluripotent media (StemCell Technologies, Vancouver, BC, Canada) supplemented with Pen/Strep. On the first day, mTeSR1 media was supplemented with 10 µM Y-27632 (S1049, Selleckchem, Houston, TX, USA) to enhance the survival of dissociated cells after passaging or cryopreservation. At 80–90% confluency, hiPSCs were either passaged to a new plate in lower density, cryopreserved or used to start a new MN differentiation. For hiPSC differentiation into MNs, we followed a protocol previously published [70 (link)] with some modifications. First, similar size squares (1–2 mm) were generated using a flame-modified Pasteur glass pipette in order to produce the clumps for embryoid body formation and cultures were treated with 3 mg/mL Collagenase type IV (Gibco, Thermo Fisher Scientific) at 37 °C. Clumps were gently resuspended in EB formation media containing Essential™6 (Gibco, Thermo Fisher Scientific) medium supplemented with 10 µM Rock inhibitor and transferred to ultra-low attachment flasks (Corning, Corning, NY, USA) on an horizontal shaker (40 rpm) in the incubator at 37 °C. For the first two days of the differentiation, neuronal basal media (DMEM/F12 Glutamax and Neurobasal supplemented with N2 and B27 without vitamin A) was supplemented with 3 µM CHIR99021 (4423, Tocris Bioscience, Bristol, UK), 0.2 µM LDN-193189 (S2618, Selleckchem), 40 µg SB431542 (1614, Tocris Bioscience), and 5 µM Y-27632 (S1049, Selleckchem). From day 3 on, neuronal basal media was supplemented with 0.1 µM retinoic acid (Sigma) and 500 nM SAG (Merck Millipore, Burlington, VT, USA). From day 8 on until the end of the differentiation BDNF (10 ng/mL, Peprotech, Waltham, MA, USA) and GDNF (10 ng/mL, Peprotech) were added to the media. From day 9 to 11, neuronal media was supplemented with 10 µM DAPT (2634, Tocris Bioscience), and from day 12–16 with 20 µM DAPT. Until day 11 included, media was changed every day. On day 11, clumps with MN progenitors were dissociated into single cells for plating on 20 µg/mL laminin coated wells. Every day, half of the media was changed. From day 17 on, maturation media containing exclusively 10 ng/mL of BDNF, GDNF and CNTF (Peprotech) was added. Every other day, media was changed by replacing half of the medium.

Muiños-Bühl A., Rombo R., Ling K.K., Zilio E., Rigo F., Bennett C.F, & Wirth B. (2023). Long-Term SMN- and Ncald-ASO Combinatorial Therapy in SMA Mice and NCALD-ASO Treatment in hiPSC-Derived Motor Neurons Show Protective Effects. International Journal of Molecular Sciences, 24(4), 4198.

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Publication 2023

1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide Cells Chir 99021 Ciliary Neurotrophic Factor Embryoid Bodies Glial Cell Line-Derived Neurotrophic Factor Homo sapiens Human Induced Pluripotent Stem Cells Induced Pluripotent Stem Cells Laminin LDN 193189 matrigel Matrix Metalloproteinase 2 Neurons Stem Cells Streptococcal Infections Tretinoin Vitamin A Y 27632

Neuronal Differentiation of Human NPCs

NPCs were differentiated from H9 human embryonic stem cells (WA09, passage 58, WiCell, Madison, WI, USA) as described before [26 (link)]. Cells were tested for mycoplasma infections annually. NPCs were thawed and cultured according to the Stemcell neuronal induction protocol (Document #28782, Stemcell Technologies, Vancouver, BC, Canada). Specifically, NPCs were maintained in STEMdiff™ Neural Progenitor (NP) medium (Stemcell) on Poly-L-Ornithine (PLO, 15 µg/mL, Sigma-Aldrich, Saint Louis, MO, USA)—laminin (10 µg/mL, Sigma-Aldrich) coated 6-well plates (Corning, New York, NY, USA) in a humidified chamber (37 °C, 5% CO₂, 3% O₂). The cells received daily refreshments for one week until they reached 100% confluency. To start the differentiation, NPCs were dissociated and seeded at 2.56 × 10⁵ cells/cm² on either PLO-laminin-coated 12/24/48-well plates (Corning) or 8-well micro-slides (Ibidi, Gräfelfing, Germany) in NP medium, depending on the type of experiment. After one day, the medium was fully replaced for neural differentiation (ND) medium adapted from [45 (link)]. ND medium was comprised of neurobasal medium, 20 µL/mL B-27 without retinoic acid supplement (50×), 10 µL/mL N2 supplement (100×), 10 µL/mL nonessential amino acids (100×), 10 µL/mL 5000 IU/mL Penicillin/5000 µg/mL Streptomycin, 20 ng/mL recombinant glial cell line-derived neurotrophic factor (GDNF), and 20 ng/mL recombinant brain-derived neurotrophic factor (BDNF; all Gibco, Waltham, MA, USA), 200 μM ascorbic acid (Sigma-Aldrich), 1 μM dibutyryl cyclic adenosine monophosphate (Sigma-Aldrich), and 2 μg/mL laminin (Sigma-Aldrich). In the first week of differentiation, recombinant ciliary neurotrophic factor (CNTF; Gibco) was added to the ND medium at a final concentration of 10 ng/mL. Half medium refreshments were performed every 2–3 days for up to 70 days.

de Leeuw V.C., van Oostrom C.T., Zwart E.P., Heusinkveld H.J, & Hessel E.V. (2023). Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons. International Journal of Molecular Sciences, 24(4), 3608.

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Publication 2023

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Top products related to «Ciliary Neurotrophic Factor»

Brain derived neurotrophic factor (bdnf) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Sweden, France

BDNF is a recombinant protein that functions as a growth factor for various cell types. It is a member of the neurotrophin family and plays a critical role in the survival, growth, and differentiation of neurons.

B27 supplement by Thermo Fisher Scientific

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B27 supplement is a serum-free and animal component-free cell culture supplement developed by Thermo Fisher Scientific. It is designed to promote the growth and survival of diverse cell types, including neurons, embryonic stem cells, and other sensitive cell lines. The core function of B27 supplement is to provide a defined, optimized combination of vitamins, antioxidants, and other essential components to support cell culture applications.

Neurobasal medium by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, Japan, Canada, Spain, France, Italy, Switzerland, China, Australia, Israel, Ireland, Sweden, Austria

Neurobasal medium is a cell culture medium designed for the maintenance and growth of primary neuronal cells. It provides a defined, serum-free environment that supports the survival and differentiation of neurons. The medium is optimized to maintain the phenotypic characteristics of neurons and minimizes the growth of non-neuronal cells.

Penicillin streptomycin by Thermo Fisher Scientific

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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Glutamax by Thermo Fisher Scientific

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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.

Brain derived neurotrophic factor (bdnf) by R&D Systems

Sourced in United States, United Kingdom, Germany

BDNF is a recombinant human brain-derived neurotrophic factor. It is a member of the nerve growth factor family of proteins and promotes the survival of nerve cells.

Forskolin by Merck Group

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Forskolin is a lab equipment product manufactured by Merck Group. It is a compound derived from the roots of the Coleus forskohlii plant. Forskolin is used as a tool for research purposes in the laboratory setting.

Laminin by Merck Group

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Laminin is a protein component found in the extracellular matrix of cells. It plays a key role in cell attachment, differentiation, and migration processes.

N2 supplement by Thermo Fisher Scientific

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The N2 supplement is a laboratory-grade nitrogen enrichment solution used to support the growth and development of cell cultures. It provides an additional source of nitrogen to cell culture media, which is essential for cellular metabolism and protein synthesis.

Dmem f12 by Thermo Fisher Scientific

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DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.

What are the different types or variations of Ciliary Neurotrophic Factor?

Ciliary Neurotrophic Factor (CNTF) is a single protein, but it can exist in different forms or isoforms due to alternative splicing of the CNTF gene. The main isoforms are the full-length CNTF and a shorter, truncated version. These isoforms may have slightly different biological activities and functions, which researchers continue to investigate for potential therapeutic applications.

How can Ciliary Neurotrophic Factor be used to treat neurodegenerative disorders?

CNTF has shown promise in the treatment of neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. It has the ability to promote the survival and differentiation of motor neurons, sensory neurons, and retinal ganglion cells. By supporting the health and function of these neuronal cell types, CNTF may help slow the progression of neurodegenerative conditions and potentially restore some lost neurological function.

What are some common challenges in using Ciliary Neurotrophic Factor for research?

One of the key challenges with CNTF research is ensuring reproducibility and accuracy of results. Differences in research protocols, reagents, and experimental conditions can significantly impact the efficacy and outcomes of CNTF-related studies. Reasearchers need to carefully optimize their methods to account for these variables and obtain reliable, consistent data.

How can PubCompare.ai help with Ciliary Neurotrophic Factor research?

PubCompare.ai's AI-powered platform can assist Ciliary Neurotrophic Factor researchers in several ways. It allows you to efficiently screen the literature to identify the most effective protocols, and the AI-driven analysis can highlight key differences in protocol effectiveness. This helps you choose the best option for your specific research goals, improving reproducibility and accuracy. The platform's data-driven insights can be invaluable in navigatiung the complexities of CNTF research and optimizing your approach.

Are there any special considerations when working with Ciliary Neurotrophic Factor?

Yes, one important consideration when working with CNTF is the need for careful handling and storage. CNTF is a delicate protein that can be easily degraded or inactivated by factors like temperature, pH, and repeated freeze-thaw cycles. Reseachers must follow strict protocols to maintain the integrity and activity of CNTF samples, ensuring reliable and reproducible results in their experiments.

More about "Ciliary Neurotrophic Factor"

Ciliary Neurotrophic Factor (CNTF) is a crucial protein that plays a vital role in the survival and differentiation of various neuronal cell types, including motor neurons, sensory neurons, and retinal ganglion cells.
As a member of the interleukin-6 cytokine family, CNTF has been extensively studied for its therapeutic potential in the treatment of neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease.
Researchers are also exploring the use of CNTF to promote nerve regeneration and neuroprotection.
To support these research efforts, scientists often utilize a range of supplementary materials, such as BDNF (Brain-Derived Neurotrophic Factor), B27 supplement, Neurobasal medium, Penicillin/Streptomycin, GlutaMAX, Forskolin, Laminin, and N2 supplement.
These substances play crucial roles in optimizing cell culture conditions, supporting neuronal growth and development, and ensuring the reproducibility and accuracy of experiments.
The DMEM/F12 medium is another commonly used component in CNTF-related research, providing a nutrient-rich environment for cell cultures.
By carefully selecting and combining these various supplements and media, researchers can create the ideal conditions to investigate the therapeutic potential of CNTF and unlock new insights into its mechanisms of action.
Optimizing research protocols is essential for ensuring the reliability and reproducibility of CNTF-related studies.
AI-powered platforms, such as PubCompare.ai, can assist researchers in identifying the best protocols and products from literature, pre-prints, and patents, enabling data-driven decisions and accelerating the progress of CNTF-focused research.
With the aid of these innovative tools, scientists can confidently explore the therapeutic applications of CNTF and drive advancements in the understanding and treatment of neurodegenerative disorders.