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Collagen Type X

Collagen Type X is a specialized type of collagen found primarily in the cartilage of growing bones.
It plays a crucial role in the process of endochondral ossification, which is the formation of bone from cartilage.
Collagen Type X is often used as a marker for hypertrophic chondrocytes, which are cartilage cells undergoing a transformation to become bone cells.
Understanding the function and regulation of Collagen Type X is important for research on skeletal development, bone disorders, and cartilage-related diseases.
Experence the power of PubCompare.ai's AI-driven platform to enhance your research on this important collagen type.

Most cited protocols related to «Collagen Type X»

1
Characterization of Chondrogenic and Osteogenic Differentiation
Cited 10 times
Monolayer and aggregate cultures were harvested and fixed in 10% buffered formalin. Aggregates were dehydrated in graded alcohols, embedded in paraffin, and sectioned (5 μm). Samples were processed for transgene expression by immunocytochemical and immunohistochemical analyses using specific antibodies. Sections were also stained with H&E (cellularity), toluidine blue (matrix proteoglycans), and alizarin red (matrix mineralization) according to routine protocols. Expression of type I, type II, and type X collagen was detected by immunohistochemistry by using specific antibodies, biotinylated secondary antibodies, and the ABC method with diaminobenzidine (DAB) as the chromogen [16 (link),37 (link),38 (link)]. To control for secondary immunoglobulins, samples were processed with omission of the primary antibody. Osteogenically differentiated cultures were stained for ALP (Alkaline Phosphatase staining kit, Sigma), and adipogenically differentiated cultures for intracellular lipid droplets with Oil Red O (Sigma) [38 (link),46 (link),47 (link)]. Samples were examined with light microscopy (Olympus BX 45, Hamburg, Germany).
Venkatesan J.K., Ekici M., Madry H., Schmitt G., Kohn D, & Cucchiarini M. (2012). SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells. Stem Cell Research & Therapy, 3(3), 22.
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Publication 2012
Alkaline Phosphatase Antibodies azo rubin S Cells Collagen Type X Ethanol Formalin Immunoglobulins Immunohistochemistry Light Microscopy Lipid Droplet Paraffin Embedding Physiologic Calcification Proteoglycan Protoplasm solvent red 27 Tolonium Chloride Transgenes
2
Characterization of Cell Culture
Cited 8 times
Cultures were harvested with selective papain digestion for aggregates. Cell proliferation was assessed with the Cell Proliferation reagent WST-1 [16 (link),44 (link)]. The DNA contents were determined with a fluorimetric assay by using Hoechst 33258 [16 (link),35 (link),41 (link)-44 (link)]. The proteoglycan contents were measured by binding to dimethylmethylene blue dye [16 (link),35 (link),41 (link)-44 (link)], and those for type I, type II, and type X collagen with ELISA [16 (link),41 (link)-44 (link)]. The ALP activities were analyzed with a colorimetric assay to measure the hydrolysis of p-nitrophenol by using a standard curve made of this reagent [16 (link)]. All measurements were performed by using a GENios spectrophotometer/fluorometer (Tecan, Crailsheim, Germany).
Venkatesan J.K., Ekici M., Madry H., Schmitt G., Kohn D, & Cucchiarini M. (2012). SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells. Stem Cell Research & Therapy, 3(3), 22.
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Publication 2012
Biological Assay Cell Proliferation Collagen Type X Colorimetry Digestion dimethylmethylene blue Enzyme-Linked Immunosorbent Assay Fluorometry Hoechst 33258 Hydrolysis Nitrophenols Papain Proteoglycan
3
Quantifying Chondrogenic Differentiation Markers
Cited 7 times
The intensities of SOX9 immunostaining and the percentages of areas stained for ALP or Oil Red O were calculated as being the ratios of positively stained surface to the total surface evaluated. The cell numbers and viability were monitored with trypan blue exclusion and by counting cells on H&E-stained sections from aggregates [16 (link),35 (link),41 (link)-44 (link)]. The intensities of SOX9 immunostaining, transduction efficiencies (X-Gal staining), aggregate diameters, cell densities, intensities of toluidine blue and of alizarin red staining, and those of type I, type II, and type X collagen immunostaining were measured at three standardized sites or by using 10 serial histologic and immunohistochemical sections for each parameter, test, and replicate condition by using SIS AnalySIS (Olympus), Adobe Photoshop (Adobe Systems, Unterschleissheim, Germany), and Scion Image (Scion Corporation, Frederick, MD, USA) [16 (link),35 (link),41 (link)-44 (link)]. The toluidine blue staining intensities were in pixels per area, and those for alizarin red staining, type I, type II, and type X collagen immunostaining in percentages, representing the ratios of positively stained tissue surface to the total surface of the site evaluated.
Venkatesan J.K., Ekici M., Madry H., Schmitt G., Kohn D, & Cucchiarini M. (2012). SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells. Stem Cell Research & Therapy, 3(3), 22.
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Publication 2012
5-bromo-4-chloro-3-indolyl beta-galactoside Collagen Type X DNA Replication solvent red 27 SOX9 protein, human Tissue Stains Tolonium Chloride Trypan Blue
4
Quantitative Analysis of Chondrogenic and Osteogenic Markers
Cited 7 times
Total cellular RNA was extracted from the cultures by using the RNeasy Protect Mini Kit with an on-column RNase-free DNase treatment (Qiagen, Hilden, Germany) [16 (link),37 (link),46 (link)]. RNA was eluted in 30 μl RNase-free water. Reverse transcription was carried out with 8 μl of eluate by using the 1st Strand cDNA Synthesis kit for RT-PCR (AMV) (Roche Applied Science). An aliquot of the cDNA product (2 μl) was amplified with real-time PCR by using the Brilliant SYBR Green QPCR Master Mix (Stratagene, Agilent Technologies, Waldbronn, Germany) [16 (link)] on an Mx3000P QPCR operator system (Stratagene) as follows: (95°C, 10 minutes), amplification by 40 cycles (denaturation at 95°C, 30 seconds; annealing at 55°C, 1 minute; extension at 72°C, 30 seconds), denaturation (95°C, 1 minute), and final incubation (55°C, 30 seconds). The primers (Invitrogen GmbH) used were SOX9 (chondrogenic marker) (forward 5'-ACACACAGCTCACTCGACCTTG-3'; reverse 5'-GGGAATTCTGGTTGGTCCTCT-3'), type II collagen (COL2A1) (chondrogenic marker) (forward 5'-GGACTTTTCTCCCCTCTCT-3'; reverse 5'-GACCCGAAGGTCTTACAGGA-3'), type I collagen (COL1A1) (osteogenic marker) (forward 5'-ACGTCCTGGTGAAGTTGGTC-3'; reverse 5'-ACCAGGGAAGCCTCTCTCTC-3'), type X collagen (COL10A1) (marker of hypertrophy) (forward 5'-CCCTCTTGTTAGTGCCAACC-3'; reverse 5'-AGATTCCAGTCCTTGGGTCA-3'), alkaline phosphatase (ALP) (osteogenic marker) (forward 5'-TGGAGCTTCAGAAGCTCAACACCA-3'; reverse 5'-ATCTCGTTGTCTGAGTACCAGTCC-3'), matrix metalloproteinase 13 (MMP13) (marker of terminal differentiation) (forward 5'-AATTTTCACTTTTGGCAATGA-3'; reverse 5'-CAAATAATTTATGAAAAAGGGATGC-3'), osteopontin (OP) (osteogenic marker) (forward 5'-ACGCCGACCAAGGAAAACTC-3'; reverse 5'-GTCCATAAACCACACTATCACCTCG-3'), runt-related transcription factor 2 (RUNX2) (osteogenic marker) (forward 5'-GCAGTTCCCAAGCATTTCAT-3'; reverse 5'-CACTCTGGCTTTGGGAAGAG-3'), β-catenin (mediator of the Wnt signaling pathway for osteoblast lineage differentiation) (forward 5'-CAAGTGGGTGGTATAGAGG-3'; reverse 5'-GCGGGACAAAGGGCAAGA-3'), parathyroid hormone-related protein (PTHrP) (hypertrophy-associated gene) (forward 5'-CGACGACACACGCACTTGAAAC-3'; reverse 5'-CGACGCTCCACTGCTGAACC-3'), lipoprotein lipase (LPL) (adipogenic marker) (forward 5'-GAGATTTCTCTGTATGGCACC-3'; reverse 5'-CTGCAAATGAGACACTTTCTC-3'), peroxisome proliferator-activated receptor gamma 2 (PPARG2) (adipogenic marker) (forward 5'-GCTGTTATGGGTGAAACTCTG-3'; reverse 5'-ATAAGGTGGAGATGCAGGCTC-3'), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (housekeeping gene and internal control) (forward 5'-GAAGGTGAAGGTCGGAGTC-3'; reverse 5'-GAAGATGGTGATGGGATTTC-3') (all 150 nM final concentration) [13 (link),15 (link),16 (link),46 (link)-49 (link)]. Control conditions included reactions using water and non-reverse-transcribed mRNA. Specificity of the products was confirmed by melting curve analysis and agarose gel electrophoresis. The threshold cycle (Ct) value for each gene of interest was measured for each amplified sample by using the MxPro QPCR software (Stratagene), and values were normalized to GAPDH expression by using the 2-ΔΔCt method, as previously described [16 (link)].
Venkatesan J.K., Ekici M., Madry H., Schmitt G., Kohn D, & Cucchiarini M. (2012). SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells. Stem Cell Research & Therapy, 3(3), 22.
Full text: Click here
Publication 2012
Adipogenesis Alkaline Phosphatase Anabolism beta-Catenin brilliant green Cells Chondrogenesis Collagen Type I Collagen Type II Collagen Type X Deoxyribonuclease I Differentiation Antigens DNA, Complementary Electrophoresis, Agar Gel Endoribonucleases Genes Genes, Housekeeping Glyceraldehyde-3-Phosphate Dehydrogenases Hypertrophy Lipoprotein Lipase Matrix Metalloproteinase 13 Neoplasm Metastasis Oligonucleotide Primers Osteoblasts Osteogenesis Osteopontin PPAR gamma PTHLH protein, human Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription RNA, Messenger RUNX2 protein, human SOX9 protein, human Training Programs Wnt Signaling Pathway
5
Skeletal Preparations and Histological Analyses
Cited 7 times
Skeletal preparations were generated as described (Ivkovic et al., 2003 (link); Yoon et al., 2006 (link)). Alcian Blue/nuclear Fast Red staining was performed as described (Luna, 1992 ). Von Kossa staining was performed by incubation in 1% silver nitrate under UV light for 20 minutes and counterstaining with nuclear Fast Red. Safranin O staining was performed by staining in Weigert's iron hematoxylin solution for 10 minutes, followed by Fast Green (0.001%) and Safranin O (0.1%) for 5 minutes each.
For immunofluorescence, sections were boiled for 15 minutes in citrate buffer (Ivkovic et al., 2003 (link)). Sections were blocked with 5% goat or donkey serum for 1 hour and incubated with primary antibody overnight at 4°C, followed by incubation with secondary antibody for 1 hour at room temperature, then with fluorophore for 30 minutes at room temperature. Primary antibodies were as follows: phospho-Smad1/5/8 and phospho-Smad1/5 (Cell Signaling Technology); type II collagen and Pth1r (Abcam); type I collagen (Southern Biotech); type X collagen (a kind gift from Robin Poole, Shriners Hospitals for Children, Montreal, Québec, Canada); aggrecan (Developmental Studies Hybridoma Bank, Iowa City, USA); Pcna (Zymed); Fgfr1 and Stat1 (Sigma); phospho-Smad1L (a kind gift from Eddy De Robertis, University of California, Los Angeles, CA, USA). Secondary antibodies were conjugated with AlexaFluor-555 and AlexaFluor-488. Sections were counterstained with DAPI (Vectashield). For TUNEL staining, the fluorescein In Situ Cell Death Detection Kit (Roche) was used according to the manufacturer's protocol. In situ hybridization was performed as described (Song et al., 2007 ).
Retting K.N., Song B., Yoon B.S, & Lyons K.M. (2009). BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development (Cambridge, England), 136(7), 1093-1104.
Publication 2009
Aggrecans Alcian Blue Antibodies Buffers Cell Death Citrates Collagen Type I Collagen Type II Collagen Type X DAPI Equus asinus Fast Green FGFR1 protein, human Fluorescein Fluorescent Antibody Technique Goat Hematoxylin Hybridomas Immunoglobulins In Situ Hybridization In Situ Nick-End Labeling Iron Proliferating Cell Nuclear Antigen Robins safranine T Serum Silver Nitrate Skeleton STAT1 protein, human Ultraviolet Rays

Most recents protocols related to «Collagen Type X»

1
Quantification of Chondrocyte Gene Expression
Total RNA was isolated from ATDC5 cells using TRizol Reagent (Thermo Fisher, Winsor, NJ, United States) and reverse transcribed according to the manufacturer’s protocol using iScript reverse transcription supermix cDNA synthesis kit (Bio-Rad, Hercules, CA, United States) 14 days after treatment. qRT-PCR was performed using the Applied Biosystems™ SYBR™ Green Assay Kit (Thermo Fisher, Winsor, NJ, United States) and Applied Biosystems StepOnePlus RT-PCR thermocycler (Applied Biosystems, San Francisco, CA, United States). Francisco, CA, United States). Primers were designed using PRIMER-Blast (NCBI) and the sequences are listed in Table 2. The evaluated mRNAs were the inflammation marker interleukin-6 (Il6), the hypertrophic chondrocyte marker type X collagen (colXa1), matrix metalloproteinase-13 (mmp13), and the chondrocyte markers type II collagen (colIIa1) and Aggrecan (acan). To evaluate the inhibitory effect of losartan on TGF-β1, mRNA expression of tgfβ1 was also evaluated. These genes of interest were normalized to the expression of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (gapdh) expression. Relative gene expression was calculated compared to the control culture condition (no nanofibers or losartan) using the 2−ΔΔCT method (n = 8 per group).
Yamaura K., Sather N.A., Metlushko A., Nishimura H., Pavlović R.Z., Hambright S., Ravuri S.K., Philippon M.J., Stupp S.I., Bahney C.S, & Huard J. (2023). Sustained-release losartan from peptide nanofibers promotes chondrogenesis. Frontiers in Bioengineering and Biotechnology, 11, 1122456.
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Publication 2023
Aftercare Aggrecans Anabolism Biological Assay Cells Chondrocyte Collagen Type II Collagen Type X DNA, Complementary Gene Expression Genes Genes, Housekeeping Glyceraldehyde-3-Phosphate Dehydrogenases Hypertrophy Inflammation Interleukin-6 Losartan Matrix Metalloproteinase 13 Oligonucleotide Primers Psychological Inhibition Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription RNA, Messenger SYBR Green I TGF-beta1 trizol
2
Immunohistochemical Analysis of Callus Formation
To examine the localization of Cnmd, type II collagen and type X collagen, and CD31 in the lengthened callus, immunohistochemistry was performed using an avidin-biotin peroxidase detection system (Vector Lab, Burlingame, CA, USA). After blocking in 5% skim milk in PBS-T (phosphate-buffered saline containing 1% Tween 20) at 4°C for 1 hour, the sections were incubated overnight at 4°C with a primary antibody, anti-Cnmd rabbit polyclonal antibody (1:1000), anti-type II collagen rabbit polyclonal antibody (1:400: Rockland Immunochemicals, Philadelphia, PA, USA), anti-type X collagen polyclonal antibody (1:400: LSL, Japan), and anti-CD31 rabbit polyclonal antibody (1:50: Abcam, Cambridge, MA, USA). After washing with PBS-T, the sections were incubated with secondary antibodies, a biotinylated anti-rabbit IgG (Vector Lab). Reactions were visualized with diaminobenzidine as substrate (Vector Lab). The sections for Cnmd, type II collagen, type X collagen, and CD31 were counterstained with hematoxylin. Five random fields (60000 μm2) per lengthened segment were measured, on CD31 stained slides, to determine the number of newly formed vascular vessels for samples 3 and 4 weeks after osteotomy. Three specimens were included for each genotype at each time point.
Yukata K., Shukunami C., Matsui Y., Takimoto A., Goto T., Takahashi M., Mihara A., Seto T., Sakai T., Hiraki Y, & Yasui N. (2023). Chondromodulin is necessary for cartilage callus distraction in mice. PLOS ONE, 18(2), e0280634.
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Publication 2023
anti-IgG Antibodies Antibodies, Anti-Idiotypic Avidin Biotin Blood Vessel Callosities Cardiac Arrest Cloning Vectors Collagen Type II Collagen Type X Genotype Hematoxylin Immunoglobulins Immunohistochemistry Milk, Cow's Osteotomy Peroxidase Phosphates Rabbits Saline Solution Tween 20
3
Histological and Immunohistochemical Analysis of Engineered Constructs
Following the in vitro and in vivo studies, the constructs were fixed in 4% paraformaldehyde for 24 h. The in vivo constructs were decalcified using EDTA for up to 3 weeks. The constructs were then embedded in paraffin and sectioned at 6 mm thickness. After rehydration, in vitro sections were stained with safranin O/Fast Green, Alizarin Red, and Von Kossa; the in vivo sections were stained with hematoxylin and eosin (H&E) and tartrate-resistant acid phosphatase (TRAP). For immunohistochemical analysis, in vivo sections were treated with 3% hydrogen peroxide to quench endogenous peroxidase activity. For antigen retrieval, the sections were incubated with 5 mg/ml hyaluronidase for 30 min at 37°C, followed by digestion with 1 mg/mL pepsin in 0.5 M acetic acid for 30 min at 37°C, after which the sections were incubated in blocking solution (5% Bloc-Ace, Dainippon Sumitomo Pharma, Osaka, Japan) for 30 min at RT. The sections were then incubated overnight with the following primary antibodies: rabbit polyclonal type I (1:400, Proteintech), type II (1:100, Santa Cruz Biotechnology), type X (1:400, Cloud-Clone Corp.) collagens, osteocalcin (1:400, Proteintech), CD31 (1:100, Abcam), SOX-9 (1:100, Santa Cruz Biotechnology), mouse monoclonal type X collagen (1:100, eBioscience), MMP-13 (1:100, Santa Cruz Biotechnology) or normal rabbit IgG (1:100, Santa Cruz Biotechnology). Peroxidase-conjugated Histofine Simple Stain MAX PO (MULTI) or MAX-PO (R, Nichirei, Tokyo, Japan) was used for secondary antibodies. The signal was detected using the ImmPACT NovaRED Substrate kit (Vector). The circularity of cells was quantified by measuring the number of cells in five different areas (200 μm × 200 μm) each, for the periphery and center of constructs.
Yamazaki S., Hirayama R., Ikeda Y., Iseki S., Yoda T, & Ikeda M.A. (2023). Hyaluronic acid hydrogels support to generate integrated bone formation through endochondral ossification in vivo using mesenchymal stem cells. PLOS ONE, 18(2), e0281345.
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Publication 2023
Acetic Acid Antibodies Antigens Clone Cells Cloning Vectors Collagen Collagen Type X Digestion Edetic Acid Eosin Fast Green Hyaluronidase MMP13 protein, human Mus Osteocalcin Paraffin Embedding paraform Pepsin A Peroxidase Peroxide, Hydrogen Rabbits Rehydration safranine T Stains Tartrate-Resistant Acid Phosphatase
4
Chondrogenic Re-differentiation of Cells
Chondrocyte phenotype was determined under re-differentiation. For chondrogenic re-differentiation, a pellet culture assay with chondrogenic differentiation media (ChM) was performed for 21 days in a 96 deep-well format as described elsewhere [43 (link),44 (link)]. Briefly, 3 × 105 cells per pellet were used, and cultured in ChM composed of high glucose DMEM including L-glutamine (Sigma Aldrich, D6546, USA), dexamethasone (0.1 µM, Sigma Aldrich, D2915, USA), L-ascorbic acid (50 µg/mL, Sigma Aldrich, A8960, USA), L-proline (40 µg/mL, Sigma Aldrich, P5607, St. Louis, MO, USA) sodium pyruvate (0.1 mg/mL, AppliChem, A4859, Darmstadt, Germany) ITS (6.25 µg/mL, Sigma Aldrich, I1884, St. Louis, MO, USA), BSA (1.25 mg/mL, Sigma Aldrich, A9418, St. Louis, MO, USA), linoleic acid (5.35 µg/mL, Sigma Aldrich, L1012, St. Louis, MO, USA), penicillin (10 U/mL)/streptomycin (10 µg/mL) (Biochrom, A2213, Berlin, Germany) and recombinant human TGF-ß1 (10 ng/mL, Peprotech, 100-21-5, Cranbury, NJ, USA). Negative controls were cultured in the same medium but without TGF-ß1. Media change was performed every 3–4 days. Chondrogenic phenotype was determined by proteoglycan assay, type II collagen immunostaining, Alcian blue staining, as well as by expression of type II collagen, type X collagen, MMP13, Sox9 and aggrecan mRNAs.
Textor M., Hoburg A., Lehnigk R., Perka C., Duda G.N., Reinke S., Blankenstein A., Hochmann S., Stockinger A., Resch H., Wolf M., Strunk D, & Geissler S. (2023). Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation. International Journal of Molecular Sciences, 24(2), 1484.
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Publication 2023
Aggrecans Alcian Blue Ascorbic Acid Biological Assay Cells Chondrocyte Chondrogenesis Collagen Type II Collagen Type X Culture Media Dexamethasone Glucose Glutamine Homo sapiens Linoleic Acid MMP13 protein, human Penicillins Phenotype Proline Proteoglycan Pyruvate RNA, Messenger Sodium SOX9 protein, human Streptomycin
5
Quantitative PCR Analysis of Equine Joint Markers

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Publication 2023
aggrecanase Aggrecans Cell Separation Collagen Collagen Type I Collagen Type X COMP protocol DNA, Complementary DNA-Directed DNA Polymerase Ephrins Equus caballus Gene Expression Genes Genes, Housekeeping Gold Interstitial Collagenase JAG2 protein, human Matrix Metalloproteinase 3 Oligonucleotide Primers Osteocalcin Real-Time Polymerase Chain Reaction Reverse Transcription RNA, Ribosomal, 18S RUNX2 protein, human Uracil-DNA Glycosylase

Top products related to «Collagen Type X»

1
Ab34712 by Abcam
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Ab34712 is a primary antibody product from Abcam. It is a polyclonal antibody raised in rabbits against a specific antigen. The antibody is designed for use in various immunoassay applications to detect the target protein.
2
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Hyaluronidase by Merck Group
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Hyaluronidase is an enzyme used in laboratory settings. It functions by breaking down hyaluronic acid, a component of the extracellular matrix.
4
Ab58632 by Abcam
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Ab58632 is a lab equipment product offered by Abcam. It is designed for laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Further information may be available upon request.
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Ab49945 by Abcam
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Ab49945 is a primary antibody that targets the Klf4 protein. It is suitable for use in various immunoassay applications.
6
Anti type x collagen col 10 antibody by Merck Group
Sourced in Germany, United States
The Anti-type-X collagen (COL-10) antibody is a laboratory reagent used for the detection and quantification of type-X collagen in biological samples. It is a specific and sensitive tool for researchers studying the role of type-X collagen in various biological processes.
7
Trizol by Thermo Fisher Scientific
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TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
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Abc reagent by Vector Laboratories
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Rneasy mini kit by Qiagen
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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More about "Collagen Type X"

Collagen X (Col X, COL10), a specialized type of collagen found primarily in the cartilage of growing bones, plays a crucial role in the process of endochondral ossification - the formation of bone from cartilage.
This collagen is often used as a marker for hypertrophic chondrocytes, which are cartilage cells undergoing transformation into bone cells.
Understanding the function and regulation of Collagen Type X is pivotal for research on skeletal development, bone disorders, and cartilage-related diseases.
The Ab34712 antibody and TRIzol reagent can be leveraged to study Collagen X expression and extraction.
Hyaluronidase, an enzyme that breaks down hyaluronic acid, is sometimes used in conjunction with Collagen X research.
The Ab58632 and Ab49945 antibodies are also relevant tools for investigating this specialized collagen.
The Anti-type-X collagen (COL-10) antibody specifically targets and detects Collagen X.
TRIzol, a reagent used for RNA extraction, and the ABC reagent, a tool for immunohistochemistry, are additional techniques employed in Collagen X studies.
The RNeasy Mini Kit can be used to purify RNA, including that of Collagen X.
Biotinylated secondary antibodies are often utilized in conjunction with primary antibodies to amplify the signal and improve detection of Collagen X.
Leveraging the power of PubCompare.ai's AI-driven platform can enhance your research on this important collagen type.
The platform helps you locate the best protocols and products by comparing data from literature, pre-prints, and patents.
Utilizing AI-powered analysis, you can identify the most reproducible and accurate methods to advance your collagen research and gain deeper insights into skeletal development, bone disorders, and cartilage-related diseases.