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> Chemicals & Drugs > Amino Acid > Collagenase

Collagenase

Collagenase is an enzyme that catalyzes the breakdown of collagen, the primary structural protein in connective tissue.
This versatile enzyme plays a crucial role in tissue remodeling, wound healing, and various research applications.
Collagenase is widely used in cell isolation and culture procedures, as it can effectively dissociate cell-cell and cell-matrix interactions, allowing for the extraction of viable cells from tissues.
Additionally, collagenase is employed in the treatment of conditions like Dupuytren's contracture, where it helps to disrupt the abnormal collagen accumulation.
Researchers leveraging collagenase must carefully optimize its use to ensure reproducibility and accuracy in their studies.
The PubCompare.ai platform offers an AI-driven solution to identify the best collagenase protocols from literature, preprints, and patents, helping elevate research efficiency and outcomes.

Most cited protocols related to «Collagenase»

1
Isolation of Murine Cardiac Myocytes
Cited 149 times
A schematic overview of the myocyte isolation procedure is shown in Figure 2. An expanded description of the procedure, accompanied with images and videos, and complete materials list is available in the Online Data Supplement, alongside full details of additional methods applied in this study (Appendix A-ix). All animal work was undertaken in accordance with Singapore National Advisory Committee for Laboratory Animal Research guidelines. Relevant national and institutional guidelines and regulations must be consulted before commencement of all animal work.
Buffers and media were prepared as detailed in Appendix D. EDTA, perfusion, and collagenase buffers were apportioned into sterile 10 mL syringes, and sterile 27 G hypodermic needles were attached (Online Figure IA).
C57/BL6J mice aged 8 to 12 weeks were anesthetized, and the chest was opened to expose the heart. Descending aorta was cut, and the heart was immediately flushed by injection of 7 mL EDTA buffer into the right ventricle. Ascending aorta was clamped using Reynolds forceps, and the heart was transferred to a 60-mm dish containing fresh EDTA buffer. Digestion was achieved by sequential injection of 10 mL EDTA buffer, 3 mL perfusion buffer, and 30 to 50 mL collagenase buffer into the left ventricle (LV). Constituent chambers (atria, LV, and right ventricle) were then separated and gently pulled into 1-mm pieces using forceps. Cellular dissociation was completed by gentle trituration, and enzyme activity was inhibited by addition of 5 mL stop buffer.
Cell suspension was passed through a 100-μm filter, and cells underwent 4 sequential rounds of gravity settling, using 3 intermediate calcium reintroduction buffers to gradually restore calcium concentration to physiological levels. The cell pellet in each round was enriched with myocytes and ultimately formed a highly pure myocyte fraction, whereas the supernatant from each round was combined to produce a fraction containing nonmyocyte cardiac populations.
CM yields and percentage of viable rod-shaped cells were quantified using a hemocytometer. Where required, the CMs were resuspended in prewarmed plating media and plated at an applicationdependent density, onto laminin (5 μg/mL) precoated tissue culture plastic or glass coverslips, in a humidified tissue culture incubator (37°C, 5% CO2). After 1 hour, and every 48 hours thereafter, media was changed to fresh, prewarmed culture media.
The cardiac nonmyocyte fraction was collected by centrifugation (300g, 5 minutes), resuspended in fibroblast growth media, and plated on tissue-culture treated plastic, area ≈ 23 cm2 (0.5× 12-well plate) per LV, in a humidified tissue culture incubator. Media was changed after 24 hours and every 48 hours thereafter.
Ackers-Johnson M., Li P.Y., Holmes A.P., O’Brien S.M., Pavlovic D, & Foo R.S. (2016). A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. Circulation research, 119(8), 909-920.
Publication 2016
Animals Animals, Laboratory Ascending Aorta Buffers Calcium Centrifugation Chest Collagenase Culture Media Descending Aorta Dietary Supplements Digestion Edetic Acid enzyme activity Fibroblasts Forceps Gravity Heart Heart Atrium Hyperostosis, Diffuse Idiopathic Skeletal Hypodermic Needles isolation Laminin Left Ventricles Mus Muscle Cells Perfusion physiology Population Group Retreatments Rod Photoreceptors Sterility, Reproductive Syringes Tissues Ventricles, Right
2
Culturing Primary Tumor Organoids
Cited 119 times

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Publication 2014
A-83-01 Acetylcysteine Cells Collagenase Culture Media Culture Media, Conditioned Digestion Dinoprostone Epidermal growth factor FGF10 protein, human Gastrins HEPES Homo sapiens matrigel Neoplasms Niacinamide noggin protein Penicillins Recombinant Proteins Repifermin Soybeans Streptomycin Tissues Trypsin Inhibitors
3
Isolation of Normal and Neoplastic Pancreatic Ducts
Cited 72 times

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Publication 2014
Collagenase Dietary Fiber Digestion dispase Enzymes Growth Factor matrigel Neoplasm Metastasis Neoplasms Pancreas Pancreatic Duct RNA-Seq Tissues
4
Optimized Isolation of Mouse Hepatocytes
Cited 59 times

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Publication 2020
Berries BLOOD Calcium Cannulation Cell Cycle Checkpoints Cells ChIP-Chip Collagenase Dental Anesthesia Digestion Extracellular Matrix Friend Hepatocyte Liberase Liver Mice, House Percoll Perfusion Veins, Portal Venae Cavae
5
Osteocyte Isolation from Mouse Long Bones
Cited 42 times
Osteocytes were isolated from mouse long bones utilizing a modified protocol derived from the combined methods of Gu et al. and Van Der Plas et al. (33 (link),44 (link),47 (link)). Long bones (femora, tibia, and humeri) were aseptically dissected from skeletally mature 4-month-old (young) and 22-month-old (old) C57BL/6 mice (Charles River Laboratories, Wilmington, MA, USA). The bones from young and old mice were processed separately by serial digestion as described in Table 1. The bones from each individual mouse were pooled together and treated as one sample. Collagenase solution was prepared as 300 active U/mL collagenase type-IA (Sigma-Aldrich, St. Louis, MO, USA) dissolved in α-minimal essential medium (αMEM; Mediatech, Manassas, VA, USA). EDTA tetrasodium salt dehydrate (EDTA) solution (5 mM, pH = 7.4; Sigma-Aldrich) was prepared in magnesium and calcium-free Dulbecco's phosphate-buffered solution (DPBS; Mediatech) with 1% BSA (Sigma-Aldrich). All steps of the digestion took place in 8 mL solution in a six-well Petri dish, on a rotating shaker set to 200 RPM, in a 37°C and 5% CO2 humidified incubator. Following each sequential digestion, the digest solution with suspended cells was removed from the bone pieces and kept. The bone pieces were then rinsed with Hank's balanced salt solution (HBSS) three times, and the rinsate was added to the digestion solution. The combined cell suspension solution was spun down at 200× g for 5 min, the supernatant was removed from the cell pellet, and cells were resuspended in culture medium and counted. The tissue homogenizer used in this study (Medimachine; BD Biosciences, San Jose, CA, USA) was utilized with a stainless steel mincing screen with a pore size of 50 μm.
Stern A.R., Stern M.M., Van Dyke M.E., Jähn K., Prideaux M, & Bonewald L.F. (2012). Isolation and culture of primary osteocytes from the long bones of skeletally mature and aged mice. BioTechniques, 52(6), 361-373.
Publication 2012
Bones Calcium Phosphates Cells Collagenase Culture Media Digestion Edetic Acid Femur Hanks Balanced Salt Solution Humerus Hyperostosis, Diffuse Idiopathic Skeletal Magnesium Mice, Inbred C57BL Mus Osteocytes Plasma Rivers Sodium Chloride Stainless Steel Tibia Tissues

Most recents protocols related to «Collagenase»

1
Single-Cell RNA-Seq of Rat Skeletal Muscle
Cell isolations were performed as previously described in mice [37 (link)] and modified slightly for rats [28 (link)]. Briefly, the gastrocnemius muscles from WB and HS male rats were excised and placed in muscle dissociation media (MDM) (Hams F-10 (Gibco, USA), 10% Horse Serum (Thermo Fisher), 1% penicillin/streptomycin (Gibco), 800 U/ml Collagenase II (Gibco)), and minced using sterilized surgical equipment. The muscle homogenate was then incubated in MDM for 1 h at 37 °C with gentle agitation. Following incubation, samples underwent further incubation in 1000 U/ml Collagenase II (Gibco) and 11 U/ml dispase (Gibco) for 30 min at 37 °C. The single-cell suspension was passed through an 18-gauge needle approximately 10 times prior to 0.2-μm filtration. Single cells were incubated in propidium iodide to identify dying/dead cells for removal via fluorescence-activated cell sorting (Sony Biotechnology, USA). Single-cell suspensions from each group were added to a Chromium Controller (10X Genomics, USA) using the Single Cell 3’ Reagent Kit per manufacturer’s instructions and sequenced on an Illumina HiSeq platform (Novogene, USA), yielding 200 million reads/sample.
Ismaeel A., Van Pelt D.W., Hettinger Z.R., Fu X., Richards C.I., Butterfield T.A., Petrocelli J.J., Vechetti I.J., Confides A.L., Drummond M.J, & Dupont-Versteegden E.E. (2023). Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy. Skeletal Muscle, 13, 6.
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Publication 2023
ATF7IP protein, human Cell Separation Chromium Collagenase dispase Equus caballus Filtration Males Mus Muscle, Gastrocnemius Muscle Tissue Needles Penicillins Propidium Iodide Rattus norvegicus Serum Streptomycin Surgical Equipment Type II Mucolipidosis
2
Pancreatic Endocrine Cell Isolation and Sorting
Cited 1 time
The pancreases from P9-P10 pups were first perfused by 1 mg/ml collagenase in Hank’s balanced salt solution, dissected, and incubated at 37 °C for 10–12 min to release endocrine cells/islets. Digested pancreatic tissue was washed 3 × by 1% FBS in Hank’s solution. To generate single cells, the tissue was further dissociated by trypsinization as described [66 (link)]. Briefly, tissue was dissociated using 0.05% trypsin/0.53 mM EDTA at 37 °C for 5 min. Digestion was stopped by the FACS buffer (2% FBS and 10 mM EGTA in PBS [66 (link)]), and cells were then 1 × washed by FACS buffer. The pancreases microdissected from E14.5 embryos were directly trypsinized and prepared for FACS as described above. Finally, cell suspensions were filtered through 40 µm nylon mesh and immediately tdTomato+ cells were sorted using a flow cytometer (BD FACSAria™ Fusion), through a 100 µm nozzle in 20 psi, operated with BD FACSDiva™ Software (Additional file 1: Fig. S7). For RNA sequencing, 100 sorted cells were collected into individual wells of 96-well plate containing 5 µl of lysis buffer of NEB Next single-cell low input RNA library prep kit for Illumina (New England Biolabs #E6420). Plates were frozen immediately on dry ice and stored at − 80 °C. The total time from euthanasia to cell collection was ∼3 h. For the epigenetic study, on average, 4700 cells/sample at E14.5 and 14,700 cells/sample at P9 were sorted. Cell sorting was performed in the Imaging Methods Core Facility at BIOCEV.
Bohuslavova R., Fabriciova V., Lebrón-Mora L., Malfatti J., Smolik O., Valihrach L., Benesova S., Zucha D., Berkova Z., Saudek F., Evans S.M, & Pavlinkova G. (2023). ISL1 controls pancreatic alpha cell fate and beta cell maturation. Cell & Bioscience, 13, 53.
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Publication 2023
Buffers cDNA Library Cells Collagenase Digestion Dry Ice Edetic Acid Egtazic Acid Embryo Euthanasia Freezing Islets of Langerhans Nylons Pancreas Sodium Chloride System, Endocrine tdTomato Tissues Trypsin
3
Culturing Pancreatic Cancer Cell Lines
HEK293T, MIA-PaCa-2, BxPC-3, and PANC-1 cell lines were obtained from the American Type Culture Collection (ATCC). They were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (#S11150; FBS, Atlanta Biologicals), antibiotics (#P4458; Gibco) and L-glutamine (#17921004; Corning). The murine pancreatic cancer cell line KPC1 was originally described in our recent publication (Parajuli et al., 2018 (link)). The cell line was established from a KP53 mouse, which harbored KrasG12D and one conditional allele of Trp53 (LSL-KrasG12D;LSL-Trp53fl/+;Pdx1-Cre). Freshly isolated specimen from the KP53 mouse with terminal PDAC was gently dissected, minced with scissors, and digested with Dispase II at 2.4 U/ml (#4942078001; Sigma-Aldrich) and Collagenase D at 0.5 mg/ml (#11088858001; Sigma-Aldrich) for 1 h at 37°C in an atmosphere of 5% CO2. Then, cells were washed three times with PBS, suspended in RPMI 1540 containing 20% FCS, and seeded on fibronectin-coated plates. Cell colonies were subsequently passaged by trypsinization, pooled, and propagated in DMEM supplemented with 10% FBS, antibiotics, and L-glutamine. To generate the PANC-1-SMAD4KO and PANC-1-SMAD2/3KO cell lines, cells were transduced with the corresponding lentiCRISPRV2-gRNA lentiviruses, selected with puromycin (for SMAD4) or hygromycin (for SMAD2/3), and all resistant clones were pooled and expanded as a single population. Lentiviruses were produced by transfecting HEK293T cells with lentiviral constructs and the One-Step Lentivirus Packaging System as described by the manufacturer (#631275; Takara). Lentiviral particles in the conditioned media were harvested after a period of 48–72 h. The conditioned media were then cleaned of cell debris by centrifugation at 5,000×g for 15 min, filtered through a 0.45-μm filter, and used immediately for cell transduction.
Hurwitz E., Parajuli P., Ozkan S., Prunier C., Nguyen T.L., Campbell D., Friend C., Bryan A.A., Lu T.X., Smith S.C., Razzaque M.S., Xu K, & Atfi A. (2023). Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer. The Journal of Cell Biology, 222(4), e202203036.
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Publication 2023
Alleles Anophthalmia with pulmonary hypoplasia Antibiotics Atmosphere Biological Factors Cell Lines Cells Centrifugation Clone Cells Collagenase Culture Media, Conditioned dispase II Eagle Fibronectins Glutamine hygromycin A Lentivirus Mus Pancreatic Cancer PDX1 protein, human Puromycin SMAD2 protein, human SMAD4 protein, human TP53 Gene
4
Detailed Solution Composition for Cell Contractions
The composition of all solutions in this study is summarized as follows. Normal Krebs solution contained the following (in mM): 120 NaCl, 5.9 KCl, 25 NaHCO3, 1.2 NaH2PO4, 11.5 dextrose, 2.5 CaCl2, 1.2 MgCl2 (Biosharp, China). High KCl solution (96 mM) was prepared as normal Krebs but with equimolar substitution of NaCl with KCl. Oxytocin (MedChemExpress, China) was dissolved in deionized water to prepare 10-11 to 10-6 M concentration for isometric contractions. Digestion solution was prepared: 2 mg/ml type II collagenase, 1 mg/ml BSA, and 0.5 mg/ml deoxyribonuclease I was dissolved in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, American). The normal glucose medium consisted of DMEM with 5.5 mmol/L glucose, 10% fetal bovine serum (FBS) (Sigma-Aldrich, American), and 1% penicillin/streptomycin (Gibco, American) solution. The high glucose medium consisted of DMEM with 25 mmol/L glucose, 10% FBS, and 1% penicillin/streptomycin solution. CoCl2 medium was prepared in normal glucose containing 200 µmol/L CoCl2 (Sigma-Aldrich, American). High glucose with echinomycin medium was prepared in high glucose containing 10 nmol/L echinomycin (MedChemExpress, China). CoCl2 with echinomycin medium was prepared in CoCl2 medium containing 10 nmol/L echinomycin. CoCl2 with L-methionine medium was 10 µmol/L L-methionine (Sigma-Aldrich, American) dissolved in CoCl2 medium. High glucose with L-methionine medium was 10 µmol/L of L-methionine dissolved in high glucose medium. High KCl (96 mM) and oxytocin (10-7 M) were dissolved in the respective medium for cell contractions.
Li T., Fei J., Yu H., Wang X., Bai J., Chen F., Li D, & Yin Z. (2023). High glucose induced HIF-1α/TREK1 expression and myometrium relaxation during pregnancy. Frontiers in Endocrinology, 14, 1115619.
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Publication 2023
Bicarbonate, Sodium Cells Collagenase Deoxyribonuclease I Digestion Eagle Echinomycin Fetal Bovine Serum Glucose Isometric Contraction Krebs-Ringer solution Magnesium Chloride Methionine mutalipocin II Oxytocin Penicillins Sodium Chloride Streptomycin
5
Fibroblast Isolation from Wolcott-Rallison Syndrome
Skin-derived fibroblasts (Isabelle Meyts, Laboratory of Inborn Errors of Immunity, KU Leuven, Leuven, Belgium) were obtained from a healthy individual (CTR) and a patient with p.W681X/p.W681X nonsense mutation in EIF2AK3 (PERK) suffering from Wolcott-Rallison syndrome. Fibroblasts were isolated from a freshly obtained skin biopsy, treated overnight with collagenase Type III (Stemcell, 400 CDU/ml), plated in T25 culture flask in serum-free conditions. After the treatment, the cells were further maintained in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 containing 4.5 g/l glucose, 0.11 g/l sodium pyruvate and 2 mM glutamine and supplemented with 100 units/ml penicillin, 100 μg/ml streptomycin and 10% FBS.
All cells were maintained in 5% CO2 at 37°C. Cells were routinely checked for mycoplasma contaminations using the Plasmotest kit (Invivogen) according to the manufacturer’s instructions.
Sassano M.L., van Vliet A.R., Vervoort E., Van Eygen S., Van den Haute C., Pavie B., Roels J., Swinnen J.V., Spinazzi M., Moens L., Casteels K., Meyts I., Pinton P., Marchi S., Rochin L., Giordano F., Felipe-Abrio B, & Agostinis P. (2023). PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration. The Journal of Cell Biology, 222(3), e202206008.
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Publication 2023
Biopsy Cells Collagenase Eagle Fibroblasts Glucose Glutamine Mutation, Nonsense Mycoplasma Nutrients Patients Penicillins Pyruvate Response, Immune Serum Skin Sodium Stem Cells Streptomycin Wolcott-Rallison syndrome

Top products related to «Collagenase»

1
Collagenase by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Japan, France, Macao, Switzerland, China, Canada, Australia, Spain, Austria, Sao Tome and Principe, Israel, Belgium, Sweden, Ireland, Jersey, India
Collagenase is an enzyme that breaks down collagen, the primary structural protein found in the extracellular matrix of various tissues. It is commonly used in cell isolation and tissue dissociation procedures.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Dnase 1 by Merck Group
Sourced in United States, Germany, Switzerland, United Kingdom, Italy, Japan, Macao, Canada, Sao Tome and Principe, China, France, Australia, Spain, Belgium, Netherlands, Israel, Sweden, India
DNase I is a laboratory enzyme that functions to degrade DNA molecules. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively breaking down DNA strands.
4
Dnase 1 by Roche
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DNase I is a lab equipment product that serves as an enzyme used for cleaving DNA molecules. It functions by catalyzing the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, effectively breaking down DNA strands.
5
Collagenase d by Roche
Sourced in Switzerland, United States, Germany, United Kingdom, France, Japan, Canada, Australia, Ireland
Collagenase D is an enzyme solution used for the dissociation and isolation of cells from various tissues. It is a mixture of proteolytic enzymes that cleave the collagen present in the extracellular matrix, allowing for the release of individual cells.
6
Collagenase 4 by Merck Group
Sourced in United States, Germany, United Kingdom, France, China, Sao Tome and Principe, Israel, Canada, Macao, Italy, Australia, Japan, Switzerland, Senegal
Collagenase IV is a purified enzyme used to dissociate and isolate cells from various tissue types. It is effective in breaking down collagen, a major structural component of the extracellular matrix.
7
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
8
Collagenase type 2 by Worthington
Sourced in United States, United Kingdom, Jersey, Germany, Japan, Switzerland, Canada, Australia, France
Collagenase type II is an enzyme used in cell and tissue culture applications. It is responsible for the breakdown of collagen, a structural protein found in the extracellular matrix. This enzyme is commonly used to facilitate the dissociation of cells from tissues during cell isolation and harvesting procedures.
9
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
10
Collagenase 4 by Thermo Fisher Scientific
Sourced in United States, Germany, Switzerland, China, United Kingdom, Japan, France, Canada, Belgium
Collagenase IV is an enzyme used in cell biology research and tissue culture applications. It is designed to dissociate and isolate cells from a variety of tissues by breaking down the collagen in the extracellular matrix.

More about "Collagenase"

Collagenase is a versatile enzyme that plays a crucial role in tissue remodeling, wound healing, and various research applications.
This powerful enzyme, also known as matrix metalloproteinase-1 (MMP-1), catalyzes the breakdown of collagen, the primary structural protein in connective tissue.
Collagenase is widely used in cell isolation and culture procedures, as it can effectively dissociate cell-cell and cell-matrix interactions, allowing for the extraction of viable cells from tissues.
This enzyme is particularly useful in the isolation of a variety of cell types, including fibroblasts, chondrocytes, and adipocytes, among others.
In addition to its applications in cell biology, collagenase is also employed in the treatment of certain medical conditions, such as Dupuytren's contracture.
In this condition, collagenase helps to disrupt the abnormal collagen accumulation, alleviating the symptoms and improving mobility.
Researchers working with collagenase must carefully optimize its use to ensure reproducibility and accuracy in their studies.
The PubCompare.ai platform offers an AI-driven solution to identify the best collagenase protocols from literature, preprints, and patents, helping to elevate research efficiency and outcomes.
When working with collagenase, researchers may also utilize additional reagents and enzymes, such as fetal bovine serum (FBS), DNase I, Collagenase D, Collagenase IV, penicillin/streptomycin, Collagenase type II, and DMEM (Dulbecco's Modified Eagle Medium).
These complementary components can help to enhance the effectiveness of collagenase-based protocols and ensure the optimal isolation and cultivation of cells.
By leveraging the insights and tools provided by PubCompare.ai, researchers can streamline their collagenase-related studies, leading to more reliable and impactful findings.