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CR3022

CR3022 is a potent neutralizing antibody that binds to the SARS-CoV-2 spike protein, potentially inhibiting viral entry into host cells.
This AI-driven platform, PubCompare.ai, helps researchers optimize their research protocols for CR3022 by easily locating relevant protocols from literature, preprints, and patents, and using intelligent comparisons to identify the best protocols and products.
This enhances reproducibility and acuuracy in CR3022 research, supporting more effective and reliable studies.

Most cited protocols related to «CR3022»

1
Isolation and Characterization of SARS-CoV Antibodies
Cited 24 times
The mAb CR3014 was isolated from a semisynthetic single-chain variable antibody fragment (scFv) phage display library, expressed as human IgG1 molecules and purified as described previously [
22 (link),
27 (link)]. An immune scFv phage display library was constructed from lymphocytes of a convalescent SARS patient from Singapore essentially as described [
28 (link)]. From this library, CR3022 scFv was selected for binding to UV-inactivated SARS-CoV, essentially as described [
22 (link)]. SARS-CoV (Frankfurt 1 strain [FM1]) was prepared as described and UV-irradiated for 15 min (UVB radiation, 280–350 nm; λ
max, 306 nm) at 4 °C. CR3022 scFv was converted into a human IgG1 format and expressed and purified as described. Anti-rabies mAb CRJA served as negative control.
ter Meulen J., van den Brink E.N., Poon L.L., Marissen W.E., Leung C.S., Cox F., Cheung C.Y., Bakker A.Q., Bogaards J.A., van Deventer E., Preiser W., Doerr H.W., Chow V.T., de Kruif J., Peiris J.S, & Goudsmit J. (2006). Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants. PLoS Medicine, 3(7), e237.
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Publication 2006
cDNA Library CR3022 Homo sapiens Hydrophobia IgG1 Immunoglobulins Lymphocyte Patients Phage Display Techniques Radiation Severe Acute Respiratory Syndrome Severe acute respiratory syndrome-related coronavirus Single-Chain Antibodies Strains
2
ELISA-based Competition Assay for Antibody Binding
Cited 18 times
Direct ELISA using the recombinantly expressed S fragment comprising amino acids 318–510 (S318–510) was performed with the mAbs as described [
22 (link)]. Competition ELISA was performed as follows. Anti-Myc-captured S318–510 fragment of the Frankfurt 1 strain was incubated with nonsaturating amounts of biotinylated IgG in the presence or absence of competing IgG. Bound biotinylated IgG was detected with streptavidin-conjugated-HRP (BD Pharmingen, San Diego, California, United States). Alternatively, S318–510 was captured by CR3014 or CR3022, detected using a biotinylated CR3014 and CR3022, and developed as described above.
ter Meulen J., van den Brink E.N., Poon L.L., Marissen W.E., Leung C.S., Cox F., Cheung C.Y., Bakker A.Q., Bogaards J.A., van Deventer E., Preiser W., Doerr H.W., Chow V.T., de Kruif J., Peiris J.S, & Goudsmit J. (2006). Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants. PLoS Medicine, 3(7), e237.
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Publication 2006
Amino Acids CR3022 Enzyme-Linked Immunosorbent Assay Monoclonal Antibodies Strains Streptavidin
3
SARS-CoV-2 Antibody-Dependent Cellular Cytotoxicity Assay
Cited 16 times

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Publication 2021
CCR5 protein, human Cell Proliferation Cells COVID 19 CR3022 Cytotoxicities, Antibody-Dependent Cell Formalin Immunoglobulins Monoclonal Antibodies Parent Plasma SARS-CoV-2 Technique, Dilution Trees
4
SARS-CoV-2 Neutralization Assay Protocol
Cited 16 times

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Publication 2020
anti-IgG Antibodies, Anti-Idiotypic Cells CR3022 Goat Homo sapiens Immunoglobulins Methylcellulose Monoclonal Antibodies paraform Peroxidase prisma Proteins Saponin SARS-CoV-2 Serum Technique, Dilution Virus
5
SARS-CoV-2 RBD/CR3022 Complex Structure
Cited 10 times
The high resolution and lower resolution coordinates and structure factors of the SARS-CoV-2 RBD/CR3022 complex are available from the PDB with accession codes PDB:6YLA andPDB:6YM0 respectively (https://www.rcsb.org/). EM maps and structure models are deposited in EMDB and PDB with accession codes EMDB:EMD-11119 andPDB:6Z97 for the prefusion spike, and EMDB:EMD-10863 and PDB:6YOR for the dimeric RBD/CR3022 complex respectively (https://www.emdataresource.org/). The data that support the findings of this study are available from the corresponding authors on request.
Huo J., Zhao Y., Ren J., Zhou D., Duyvesteyn H.M., Ginn H.M., Carrique L., Malinauskas T., Ruza R.R., Shah P.N., Tan T.K., Rijal P., Coombes N., Bewley K.R., Tree J.A., Radecke J., Paterson N.G., Supasa P., Mongkolsapaya J., Screaton G.R., Carroll M., Townsend A., Fry E.E., Owens R.J, & Stuart D.I. (2020). Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike. Cell Host & Microbe, 28(3), 445-454.e6.
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Publication 2020
CR3022 Microtubule-Associated Proteins SARS-CoV-2

Most recents protocols related to «CR3022»

1
Epitope Mapping of SARS-CoV-2 Antibodies
The epitope regions of CR3022 and S309 were determined using the published structural data in protein data bank (PDB (40 (link)–43 (link))) for the antibody complexed with SARS-CoV-2 viz. PDB id. 6W41 for CR3022 (41 (link)) and PDB id. 6WPS for S309 (42 (link)). All the amino acids in antigens that are within 5Å contact of amino acids of antibodies were considered as epitope residues. The position of the glycosylation site was determined by in-silico mutation of triplets of amino acids in the epitopes to glycosylation sequon – N-X-T (44 (link)) using the FoldX algorithm (43 (link)). Briefly, residues succeeding N-X motif, where X can be any amino acid except Pro, were mutated to either Threonine or Serine or residues preceding X-T, where X can be any amino acid except Pro, were mutated to Asn to generate novel N-X-T/S motifs. The mutations with the least energy cost, as calculated by the Build module of FoldX, were selected for designing M7 and M8.
Carnell G.W., Billmeier M., Vishwanath S., Suau Sans M., Wein H., George C.L., Neckermann P., Del Rosario J.M., Sampson A.T., Einhauser S., Aguinam E.T., Ferrari M., Tonks P., Nadesalingam A., Schütz A., Huang C.Q., Wells D.A., Paloniemi M., Jordan I., Cantoni D., Peterhoff D., Asbach B., Sandig V., Temperton N., Kinsley R., Wagner R, & Heeney J.L. (2023). Glycan masking of a non-neutralising epitope enhances neutralising antibodies targeting the RBD of SARS-CoV-2 and its variants. Frontiers in Immunology, 14, 1118523.
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Publication 2023
Amino Acids Antibodies Antigens CR3022 Epitopes Immunoglobulins Mutation Protein Glycosylation SARS-CoV-2 Serine Threonine Triplets
2
Kinetics of yeast-displayed scFv-trimeric HA
Kinetics measurements were acquired on an Octet RED96e (Sartorius). To mimic the interaction between yeast-displayed scFv and trimeric HA, IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect, IgGs were loaded to a density of ~0.1 nm using a solution of 10 nM of IgG. All binding measurements were obtained in 'kinetics' buffer: PBS supplemented with 0.1% BSA and 0.01% Tween20. Binding measurements were acquired as follows with shaking at 1000 rpm – baseline: 60 s; loading: 30 s with threshold at 0.1 nm; baseline: 60 s; association: 360 s; dissociation: 600 s. Tips were regenerated a maximum of four times by alternating between 10 mM glycine (pH 1.7) and kinetics buffer three times with 10 s in each buffer. Kinetics measurements were obtained at four temperatures for each antibody: 20, 25, 30, and 35°C. Kinetics measurements for the UCA, I-2, and CH65 were also acquired at 40°C. Prior to each measurement, the plate was allowed to equilibrate to the set temperature for 20 min. Each full-length, trimeric HA (MA90, MA90-G189E, and SI06) was assayed at six concentrations: 500, 250, 125, 62.5, 31.25, and 15.625 nM. For each antibody against each HA, antibody assayed with buffer only was used as a reference for subtraction. Additionally, each run contained an irrelevant IgG (CR3022) at the highest HA concentration (500 nM) to detect any nonspecific interaction, which was at background level. To account for the multivalency of the analyte (trimeric HA), the bivalent analyte model was used for global curve fitting in the Sartorius Data Analysis HT software version 12.0.2.59.
Phillips A.M., Maurer D.P., Brooks C., Dupic T., Schmidt A.G, & Desai M.M. (2023). Hierarchical sequence-affinity landscapes shape the evolution of breadth in an anti-influenza receptor binding site antibody. eLife, 12, e83628.
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Publication 2023
Biosensors Buffers CR3022 Glycine Homo sapiens Immunoglobulins Kinetics Tween 20 Yeast, Dried
3
SARS-CoV-2 and RSV Viral Plaque Assays

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Publication 2023
A549 Cells Antibodies Biological Assay Cells CR3022 Formaldehyde Methylcellulose Monoclonal Antibodies Rabbits SARS-CoV-2 Senile Plaques Serum Vero Cells Violet, Gentian Viral Plaque Assay Viscosity
4
Nanoparticle-Antibody Binding Assay

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Publication 2023
3,3',5,5'-tetramethylbenzidine ACE2 protein, human anti-IgG Buffers Carbonates CR3022 Homo sapiens Immunoglobulins Peroxidase REGN-10933
5
Quantification of SARS-CoV-2 Spike RBD IgG
Serum immunoglobulin G SARS-CoV-2 spike RBD-specific antibodies were quantified as described in detail (6 (link)). Briefly, duplicate serum samples were added to 96-well microtiter plates that had been coated with recombinant RBD protein. After washing, goat anti-human IgG conjugated with horseradish peroxidase was added, followed by washing and addition of tetramethylbenzidine substrate. Measurements were performed at 450 and 650 nm, and the results were compared to a standard curve generated by a control titration of the anti-RBD monoclonal antibody CR3022 (Creative Biolabs, Shirley, NY). Serum anti-RBD IgG binding activity was expressed as equivalence to a concentration of CR3022.
Taus E., Hofmann C., Ibarrondo F.J., Gong L.S., Hausner M.A., Fulcher J.A., Krogstad P., Kitchen S.G., Ferbas K.G., Tobin N.H., Rimoin A.W., Aldrovandi G.M, & Yang O.O. (2023). Persistent memory despite rapid contraction of circulating T Cell responses to SARS-CoV-2 mRNA vaccination. Frontiers in Immunology, 14, 1100594.
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Publication 2023
3,3',5,5'-tetramethylbenzidine anti-IgG Antibodies CR3022 Goat Homo sapiens Horseradish Peroxidase Immunoglobulin G Monoclonal Antibodies Recombinant Proteins SARS-CoV-2 Serum Titrimetry

Top products related to «CR3022»

1
Immunospot microanalyzer by Cellular Technology
The ImmunoSpot microanalyzer is a laboratory equipment designed for the analysis of immune cells. It provides precise quantification and characterization of various immune cell subsets, such as T cells and B cells, based on their secreted proteins or cytokines.
2
Expi293f cells by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom
Expi293F cells are a suspension-adapted mammalian cell line derived from HEK293F cells. They are designed for high-level recombinant protein expression in biopharmaceutical and biotechnology applications.
3
Maxisorp plate by Thermo Fisher Scientific
Sourced in Denmark, United States, Germany, United Kingdom, Canada
Maxisorp plates are a type of microwell plate designed for immunoassays. They feature a high-binding surface that allows for efficient capture of proteins and other biomolecules. The plates are made of polystyrene and are available in a variety of well configurations to suit different experimental needs.
4
Cr3022 by Creative Biolabs
CR3022 is a recombinant human monoclonal antibody that targets the SARS-CoV-2 spike protein. It is designed for research purposes and has not been approved for clinical use.
5
Cr3022 by Absolute Antibody
Sourced in United States, United Kingdom
CR3022 is a recombinant antibody that binds to the SARS-CoV-2 spike protein. It is produced using Absolute Antibody's recombinant antibody technology.
6
Lsrii cytometer by BD
Sourced in United States, Canada, France, Germany
The BD LSRII cytometer is a flow cytometry instrument used for the analysis and sorting of cells. It is designed to detect and quantify multiple parameters of individual cells or particles in a liquid sample as they pass through a laser beam. The LSRII provides high-performance data acquisition and processing capabilities for a wide range of applications in life science research.
7
Aquavivid by Thermo Fisher Scientific
Sourced in United States
AquaVivid is a compact and versatile lab equipment designed for water analysis. It utilizes a spectrophotometric method to accurately measure a range of water quality parameters, including pH, turbidity, and the presence of various chemicals and compounds. The device provides reliable and consistent results, making it a useful tool for water testing and monitoring applications.
8
Tmb substrate by Thermo Fisher Scientific
Sourced in United States, Germany
TMB substrate is a chromogenic substrate used in enzyme-linked immunosorbent assay (ELISA) and other immunodetection methods. It is a two-component system that, when combined, produces a blue color upon oxidation by the enzyme horseradish peroxidase (HRP). The intensity of the blue color is proportional to the amount of HRP present, allowing for quantitative analysis of target analytes.
9
Anti human igm igg iga by Jackson ImmunoResearch
Anti-human IgM+IgG+IgA is a laboratory reagent that can detect and measure the presence of human immunoglobulins IgM, IgG, and IgA in various samples. It is commonly used in immunological assays and tests.
10
Amersham imager 600 by GE Healthcare
Sourced in United States, United Kingdom, Japan, China, Sweden, Germany, Spain, France, Switzerland, Belgium, New Zealand
The Amersham Imager 600 is a compact and versatile imaging system designed for a wide range of life science applications. It features a high-resolution CCD camera and a selection of interchangeable emission filters to capture, analyze, and document fluorescent, chemiluminescent, and colorimetric signals.

More about "CR3022"

CR3022 is a potent neutralizing antibody that binds to the SARS-CoV-2 spike protein, potentially inhibiting viral entry into host cells.
This AI-driven platform, PubCompare.ai, helps researchers optimize their research protocols for CR3022 by easily locating relevant protocols from literature, preprints, and patents, and using intelligent comparisons to identify the best protocols and products.
This enhances reproducibility and accuarcy in CR3022 research, supporting more effective and reliable studies.
CR3022 is a valuable tool in the fight against COVID-19, as it can neutralize the SARS-CoV-2 virus and prevent it from infecting cells.
Researchers can use PubCompare.ai to streamline their CR3022 research, accessing a wealth of relevant protocols and products, and comparing them to find the most effective and accurate methods.
The ImmunoSpot microanalyzer, Expi293F cells, and Maxisorp plates are all important tools used in CR3022 research.
The LSRII cytometer and AquaVivid dye can be used to analyze the binding and neutralization properties of CR3022, while the TMB substrate and Anti-human IgM+IgG+IgA antibodies can be used to detect and quantify the antibody's presence.
By utilizing PubCompare.ai and these specialized tools and techniques, researchers can enhance the reproducibility and accuracy of their CR3022 studies, leading to more effective and reliable results.
This, in turn, can contribute to the development of more effective treatments and preventative measures against COVID-19, ultimately helping to protect public health.
The Amersham Imager 600 can be used to visualize and analyze the results of these studies, providing valuable insights into the performance and potential of CR3022 as a therapeutic agent.