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Cytokeratin 8

Cytokeratin 8 is a type II intermdiate filament protein that is expressed in simple and stratified epithelial cells.
It forms heterodimers with cytokeratin 18 and plays a role in maintaining the structural integrity of epithelial cells.
Cytokeratin 8 is often used as a marker for epithelial differentiation and is implicated in various cancers and disease states.
Researchers can optimize their Cytokeratin 8 studies using PubCompare.ai's intelligent platform, which helps locate relevant protocols from literature, preprints, and patents, and leverages AI-powered comparisons to identify the most reproducible and accurate results.
This streamlined research solution offers an efficient way to advance Cytokeratin 8-related investigations.

Most cited protocols related to «Cytokeratin 8»

CellSearch Circulating Tumor Cell Enumeration

Cited 11 times

Circulating tumour cell enumeration using the CellSearch system (Veridex LLC, Raritan, NJ, USA) was carried out according to the manufacturer's protocol and training. Blood samples were processed on the CellTracks Autoprep within 72 h. Epithelial cells were immunomagnetically enriched using ferrofluids coated with EpCAM-specific antibodies, then permeabilised and fluorescently labelled with phycoerythrin-conjugated antibodies directed against cytokeratin 8, 18 and 19, an allophycocyanin-conjugated antibody to CD45 and the nuclear dye DAPI. After transfer to a cartridge in a MagNest, labelled cells were analysed on the CellTracks Analyser II, a four-colour semi-automated fluorescent microscope that captures images of the entire surface of the cartridge for the four fluorescent dyes. From the captured images, a gallery of objects was presented to a trained operator (technician) who interprets each object. According to manufacturer guidelines, an object defined as a CTC should meet all of the following criteria: (i) an intact cell with a round to oval morphology and at least 4 μm in size; (ii) positive for DAPI with a nucleus inside the cytoplasm (of at least >50%) and a nucleus area smaller than the cytoplasm; (iii) positive for cytokeratins (bright or moderate) and negative for CD45 and the blank channel. Each sample was analysed independently by two trained technicians (NV and NJ).

Farace F., Massard C., Vimond N., Drusch F., Jacques N., Billiot F., Laplanche A., Chauchereau A., Lacroix L., Planchard D., Le Moulec S., André F., Fizazi K., Soria J.C, & Vielh P. (2011). A direct comparison of CellSearch and ISET for circulating tumour-cell detection in patients with metastatic carcinomas. British Journal of Cancer, 105(6), 847-853.

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Publication 2011

allophycocyanin Antibodies BLOOD Cell Nucleus Cells Circulating Neoplastic Cells Cytokeratin Cytokeratin 8 Cytoplasm DAPI Epithelial Cells Fluorescent Dyes Immunoglobulins Microscopy Phycoerythrin TACSTD1 protein, human

Fluorescent Immunoassay for CTC Detection

Cited 9 times

MCF-7, SKBR3 and MD-MBA-453 breast cell lines and PC3 and LNCaP prostate cell lines were used to develop a fluorescent immunoassay for CTC detection. The final design consisting of 7 μm diameter pores array patterned with the 160 000 porosity was used. Trypsinized cells suspended in media (concentration ~10^4 cell per mL) were diluted 1 : 1 in an Iodine solution (Fisher Scientific). Approximately 50 cells (25–100) were counted and spiked into 7.5 mL Blood (single donor) using 1× Prefixation Buffer. Samples were incubated for 15 minutes and run according to Negative Pressure System Setup. Filters were then fixed in 1× Postfixation Buffer for 15 min, permeabilized in 1× Permeabilization Buffer for 15 min and washed with 1× PBS. An antibody cocktail (Creatv Microtech, Inc.) of FITC conjugated anti-cytokeratin 8, FITC conjugated anti-cytokeratin 18, FITC conjugated anti-cytokeratin 19, PE conjugated anti-EpCAM (Epithelial Cell Adhesion Molecule), and Cy5 conjugated anti-CD45 was placed on the filter for 1 hour and then washed with 1× PBS + 0.1% Tween-20. Filters were then mounted using Fluoromount-G with DAPI (Southern Biotech). CTCs were identified on the filter by the presence of FITC, EpCAM, DAPI and lack of Cy5 signal, all cell lines were run in triplicate.
An Olympus BX54WI Fluorescent microscope with a Carl Zeiss AxioCam was used to image all of the filters after fluorescent staining. Exposures were set for 5 s for Cy5, 2 s for PE, 750 msec for FITC, and 10-50 msec for DAPI. A Zen 2011 Blue Edition (Carl Zeiss) was used to process the images.

Adams D.L., Zhu P., Makarova O.V., Martin S.S., Charpentier M., Chumsri S., Li S., Amstutz P, & Tang C.M. (2014). The systematic study of circulating tumor cell isolation using lithographic microfilters. RSC advances, 9, 4334-4342.

Publication 2014

BLOOD Breast Buffers Cell Lines Cells Combined Antibody Therapeutics Cytokeratin 8 DAPI Epithelial Cell Adhesion Molecule Fluorescein-5-isothiocyanate Immunoassay Iodine Keratin-19 KRT18 protein, human Microscopy Pressure Prostate TACSTD1 protein, human Tissue Donors Tween 20

Metastatic Breast Cancer CTC Isolation

Cited 8 times

Blood samples from healthy volunteers were purchased through Biological Specialty Corp or Lampire Biological Laboratories, with written informed consent and Institutional Review Board approvals. Samples were collected into Potassium EDTA as an anticoagulant and shipped at room temperature. All samples were run within 24 hours of draw time. Additionally, peripheral blood samples from 10 metastatic breast cancer patients were supplied through a collaboration agreement University of Maryland Baltimore according to the local IRB approval and with informed consent.
The optimized filter design was validated using 7.5 mL whole blood from 10 metastatic breast cancer patients. Peripheral blood was prefixed for 15 min, placed into the 30 mL syringe and drawn through the filter in rv3 min. The filter was washed with 6 mL PBS, postfixed for 15 min, and permeabilized for 15 min. The filter and cells were stained with the antibody cocktail of FITC conjugated anti-Cytokeratin 8, 18, 19; Phycoerythrin (PE) conjugated EpCAM; and Cy5 conjugated CD45 (Creatv Microtech, Inc.). Filters were washed with PBS + 0.1% Tween-20 (PBST), placed onto a microscope slide and mounted with Fluoromount-G with DAPI (Southern Biotech).

Publication 2014

Anticoagulants Biopharmaceuticals BLOOD Breast Cells Combined Antibody Therapeutics Cytokeratin 8 DAPI Edetic Acid, Potassium Salt Fluorescein-5-isothiocyanate Healthy Volunteers Hematologic Neoplasms Malignant Neoplasm of Breast Microscopy Neoplasm Metastasis Patients Phycoerythrin Syringes TACSTD1 protein, human Tween 20

Immunophenotypic analysis of CDV-infected cells

Cited 7 times

An initial qualitative immunohistochemical screening of the expression of E‐cadherin, β‐catenin, cytokeratin 8 (CK8), vimentin and N‐cadherin was performed on formalin‐fixed paraffin‐embedded (FFPE) cell pellets of both non‐infected and persistently CDV‐infected DH82 cells (Supplementary material and Table S2).
Non‐infected DH82 and DH82Ond pi cells were seeded at a density of 0.03 * 10⁶ cells/0.33 cm² into 96 Microwell Nunc plates (Nunc GmbH & Co. KG, Thermo Scientific). All the immunostainings were performed in triplicates with negative controls in duplicates. 3 days after seeding, cells were fixed with 4% paraformaldehyde and immunofluorescence was performed according to a 2 days protocol with minor variations.²⁵ To verify the persistent CDV infection state of DH82Ond pi cells, an immunolabelling with an anti‐CDV nucleoprotein (CDV‐NP) antibody (clone D110; kindly provided by Prof. Dr A. Zurbriggen, University of Bern, Switzerland) was performed as previously described.¹³ Furthermore, cells were immunolabelled for E‐cadherin, β‐catenin and cytokeratin 8 as epithelial markers, and for vimentin and N‐cadherin as mesenchymal markers. All details regarding the aforementioned antibodies are listed in Table 1. For negative controls, the first antibody was replaced with rabbit serum, Balb/c ascitic fluid or goat serum, respectively, at corresponding protein concentrations. For all the aforementioned markers, the percentage of immunopositive cells was determined for each group (non‐infected DH82 cells and DH82Ond pi cells) by counting 5 evenly distributed fields per well, taking pictures at a 400× magnification using a fluorescence microscope (Olympus IX‐70, Olympus Optical Co. GmbH) equipped with an Olympus DP72 camera and Olympus cell sense standard software version 2.3. The analysed pictures were taken from areas of different confluence (low, medium, high) for each marker and for both persistently CDV‐infected and non‐infected DH82 cells. Besides the determination of the overall percentage of positive cells for each marker, the number of positive cells based on the cell shape (round, spindle or multinucleated giant cells) as well as the number of positive cells based on the intracellular localization (membranous, membranous to cytoplasmic, diffusely cytoplasmic, focally cytoplasmic) was additionally evaluated. Detailed descriptions of the cell shape and of the intracellular distribution pattern are available as supplementary material. Statistical analysis as well as graphical visualization was carried out using GraphPad Prism version 8.0.1 for Windows (GraphPad Software, La Jolla California USA, www.graphpad.com. The values were analysed with Student's t test, setting the significance level at P ≤ .05.

Armando F., Gambini M., Corradi A., Becker K., Marek K., Pfankuche V.M., Mergani A.E., Brogden G., de Buhr N., von Köckritz‐Blickwede M., Naim H.Y., Baumgärtner W, & Puff C. (2020). Mesenchymal to epithelial transition driven by canine distemper virus infection of canine histiocytic sarcoma cells contributes to a reduced cell motility in vitro. Journal of Cellular and Molecular Medicine, 24(16), 9332-9348.

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Publication 2020

Antibodies Antibodies, Anti-Idiotypic Ascitic Fluid Cadherins Cells Cell Shape Clone Cells CTNNB1 protein, human Cytokeratin 8 Formalin Giant Cells Goat Immunofluorescence Immunoglobulins Mesenchyma Microscopy, Fluorescence N-Cadherins Nucleoproteins Paraffin Embedding paraform Pellets, Drug Persistent Infection Plasma Membrane prisma Proteins Protoplasm Rabbits Serum Vimentin Vision

CTC Enrichment and Enumeration Protocol

Cited 7 times

Samples are filtered with a CellSieve™ Microfiltration Assay using a low-pressure vacuum system which isolates CTCs based on size exclusion, >7 micron, as previously described1 (link)2 (link)3 (link)25 (link)39 . Cells were stained and identified by fluorescent enumeration using CTC enumeration stains, as previously described. Briefly, a low-pressure system uses a filter holder assembly with CellSieve™ filter attached. Peripheral blood (7.5 mL), is diluted in a prefixation buffer and drawn through the filter. The filter is washed, postfixed and permeabilized. The captured cells are stained with an antibody cocktail consisting of FITC-anti-Cytokeratin 8, 18, 19, PE-anti-EpCAM and Cy5-anti-CD45 for 1 hour and mounted with Fluoromount-G/DAPI (Southern Biotech). An Olympus BX54WI Fluorescent microscope with Carl Zeiss AxioCam was used to image the samples. Exposures were preset as 2 sec (Cyanine5), 2 sec (PE), 100–750 msec (FITC), and 10–50 msec (DAPI) for equal signal comparisons between cells. A Zen2011 Blue (Carl Zeiss) with AxioVision Mark and Find module was used to process the images, mark the x/y placement of the cells, and relocate previously imaged cells in a semi-automated manner. Samples were archived and placed in storage at 4 °C for 1 week-2 years.

Adams D.L., Alpaugh R.K., Tsai S., Tang C.M, & Stefansson S. (2016). Multi-Phenotypic subtyping of circulating tumor cells using sequential fluorescent quenching and restaining. Scientific Reports, 6, 33488.

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Publication 2016

Biological Assay BLOOD Buffers Cells Combined Antibody Therapeutics Cytokeratin 8 DAPI Fluorescein-5-isothiocyanate Microscopy Pressure Signal Transduction Staining TACSTD1 protein, human Vacuum

Most recents protocols related to «Cytokeratin 8»

Immunofluorescence Staining of Caco-2 Cells

Caco-2 cells were fixed using 4% Paraformaldehyde (PFA) in cytoskeleton stabilisation buffer (10mM PIPES at pH 6.8, 100mM KCl, 300mM sucrose, 2mM EGTA and 2mM MgCl₂) for 20 min at room temperature (RT) or were fixed using 100% methanol for 10 min at −20°C. Staining for Myosin VI, DP, keratin-8, keratin-18, Dsg2, tagRFPT and GFP required pre-permeabilisation of cells (0.1% Triton in PBS) for 3 min on ice before fixation with PFA. Subsequently, the fixed cells were blocked in 3% Bovine Serum Albumin (BSA) in PBS at RT for 1 hour. Following this, cells were incubated with primary antibody for 1 hour at RT. For Myosin VI and Dsg2 primary antibodies, the cells were incubated overnight at 4°C. Then, the cells were washed with 0.05% Tween in PBS before incubating with the corresponding secondary antibodies at RT for 1 hour. Following this, the coverslips were mounted using Prolong gold (Genesearch #8916BC) with or without DAPI.

Nanavati B.N., Noordstra I., Verma S., Duszyc K., Green K.J, & Yap A.S. (2023). Desmosome-anchored intermediate filaments facilitate tension-sensitive RhoA signaling for epithelial homeostasis. bioRxiv.

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Publication Preprint 2023

Antibodies Bos taurus Buffers Caco-2 Cells Cells Cytokeratin 8 Cytokeratin 18 Cytoskeleton DAPI desmoglein 2 protein, human Egtazic Acid Gold Immunoglobulins Magnesium Chloride Methanol myosin VI paraform piperazine-N,N'-bis(2-ethanesulfonic acid) Serum Albumin Sucrose Tweens

Single-cell Characterization of Tumor and Mammary Cells

Single-cell suspensions were first stained with fluorescently labeled antibodies. Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend, 102418, clone:390, 1:100 dilution), and PE/Cyanine 7 anti-mouse CD45 antibody (Biolegend, 103114, clone: 30-F11, 1:100 dilution) for 30 min on ice. Mammary gland single-cell suspensions were stained with all the above antibodies, with the addition of Super Bright 600 anti-CD49f (integrin alpha 6) antibody (Thermo Scientific, 63-0495-42, clone: GoH3, 1:100 dilution).
For staining intracellular keratins, single-cell suspensions were fixed for 15 min at RT in 2% paraformaldehyde (methanol free, Thermo Scientific, J19943-K2), and permeabilized for 15 min at RT in Intracellular Staining Perm Wash Buffer (Biolegend, 421002), before staining with recombinant anti-cytokeratin 8 antibody Alexa Fluor 647 (Abcam, ab192468, clone: EP1628Y, 1:100 dilution), and recombinant anti-cytokeratin 5 antibody (Abcam, ab236216, clone: SP27) conjugated to Dylight 405 (Abcam, ab201798), or anti-cytokeratin 14 monoclonal antibody (Thermo Scientific, MA5-11599, clone: LL002), conjugated to Pacific Blue (Thermo Scientific, P30013) for 30 min at RT. Samples were washed and resuspended in PBS supplemented with 2% FBS before being analyzed for cell marker expression using BioRad ZE-5 cell analyzer. Compensation was performed with the aid of single-stained Ultracomp eBeads plus compensation beads (Invitrogen, 01-3333-42). Analysis and plot generation was performed on FlowJo.
For sorting of GFP-expressing cells, single-cell suspensions were only stained for extracellular markers, and DAPI (Sigma-Aldrich, 10236276001) was added at a dilution of 1:1000 after the final wash step in order to facilitate live cell sorting. GFP-positive cells were sorted on FACSAria III cell sorter by first gating on DAPI-negative live cells, and CD31- and CD45-negative epithelial cells.

Mohamed G.A., Mahmood S., Ognjenovic N.B., Lee M.K., Wilkins O.M., Christensen B.C., Muller K.E, & Pattabiraman D.R. (2023). Lineage plasticity enables low-ER luminal tumors to evolve and gain basal-like traits. Breast Cancer Research : BCR, 25, 23.

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Publication 2023

Alexa Fluor 647 Antibodies Antibodies, Anti-Idiotypic Buffers Cells Clone Cells Cytokeratin Cytokeratin 8 DAPI Epithelial Cells Germ Cells Immunoglobulins ITGA6 protein, human Keratin-5 Keratin-14 Mammary Gland Methanol Monoclonal Antibodies Mus Neoplasms paraform Progressive Encephalomyelitis with Rigidity Protoplasm TACSTD1 protein, human Technique, Dilution

Quantification of Gene Expression in Hepatocytes

From the CCl₄-treated injured cells and other treated cells, and also from liver tissues samples, RNA was extracted using an E.Z.N.A. ^® Total RNA kit, according to the kit protocol. The quantification of RNA was conducted using a Spectrophotomter Nano drop; ND-1000 and cDNA were synthesized by a BIO-RAD I Script^tmcDNA synthesis kit using RT-PCR.
Real-time PCR analysis was carried out with SYBR Green PCR Super Mix (Bio-Rad, Hercules, CA, USA) and 2 µI of cDNA. The procedure of PCR consisted of an initial period of denaturation at a temperature of 94 °C for 4 min, 35 cycles of denaturation for 45 s and then annealing at 56 °C–58 °C for 45 s and an extension at 72 °C for 45 s, followed by a final step of extension at 72 °C for 10 min. The expression levels of albumin, cyto-keratin 8, Bcl₂, BAX, Bcl-xL, TNF-α, NF-κβ and caspase-3 were measured. The comparative CT method (∆∆Ct value) was used to measure the relative expression of target genes. As a reference, gene β-actin was used. All primer sequences are given in Table 2.

Iqbal M., Shams S., Rafiq H., Khan M., Khan S., Sadique Khattak U., Afridi S.G., Bibi F., Abdulkareem A.A, & Naseer M.I. (2023). Combinatorial Therapeutic Potential of Stem Cells and Benzimidazol Derivatives for the Reduction of Liver Fibrosis. Pharmaceuticals, 16(2), 306.

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Publication 2023

Actins Albumins Anabolism BCL2 protein, human Caspase 3 CCL4 protein, human Cells Cytokeratin 8 DNA, Complementary Gene Expression Genes Liver Oligonucleotide Primers Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction SYBR Green I Tissues Tumor Necrosis Factor-alpha

Immunohistochemical Analysis of Tissue Sections

Briefly, deparaffinization and rehydration of 5 μm sections were performed as follows: 1. three incubations for 3 min in xylene; 2. three incubations for 2 min in 100% ethanol; 3. 2 min each in 95, 80, and 70% ethanol; 4. 5 min in 1× TBS (Tris-Buffered Saline). All chemicals, BSA, and buffers were purchased from Fisher Scientific, Pittsburgh, PA, USA. Antigen retrieval was performed using a citrate buffer, pH 8.0 at 98C, for 20 min, as previously reported [32 (link)]. Sections were subsequently blocked using 5% Bovine Serum Albumin (BSA), 1× TBS solution. Sections were stained with anti-Ki67-AlexaFluro-555 conjugated antibody (BD Biosciences, Franklin Lakes, NJ, USA), anti-cytokeratin 8 [33 (link)] (1:100 dilution, TROMA-I was deposited to the DSHB (Developmental Studies Hybridoma Bank) by Drs. Brulet, P. & Kemler, R.), and anti-cytokeratin5 (Poly19055, BioLegend San Diego, CA, USA), dilution 1:500, and incubated overnight, as previously reported [34 (link)]. Secondary antibodies that were diluted, 1:10,000, included AlexaFluor-488, 555, and 647 (ThermoFisher Scientific, Waltham, MA, USA). The sections were mounted with ProLong Gold DAPI-containing media (ThermoFisher Scientific, Waltham, MA, USA). Images were captured using an Olympus VS120 Slide Scanner microscope with 10× U Plan S Apo/0.75 NA objective (Olympus Lifescience, Center Valley, PA, USA).
For H&E, staining slides post deparaffinization and rehydration were incubated with hematoxylin (Fisher Scientific, Pittsburgh, PA, USA) for 45 s, rinsed with water for 30 s, followed by Eosin for 1 min, rinsed and dehydrated in 95% ethanol, followed by three incubations for 1 min in 100% ethanol and three incubations for 1 min in xylene. Slides were mounted using Richard-Allan Scientific Mounting Medium (ThermoFisher Scientific, Waltham, MA, USA). Samples were evaluated by an experienced pathologist blinded to the identity of the slides.

Purazo M.L., Ice R.J., Shimpi R., Hoenerhoff M, & Pugacheva E.N. (2023). NEDD9 Overexpression Causes Hyperproliferation of Luminal Cells and Cooperates with HER2 Oncogene in Tumor Initiation: A Novel Prognostic Marker in Breast Cancer. Cancers, 15(4), 1119.

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Publication 2023

Antibodies Antibodies, Anti-Idiotypic Antigens APO 10 Buffers Citrates Cytokeratin 8 DAPI Eosin Ethanol Gold Hematoxylin Hybridomas Microscopy Pathologists Rehydration Saline Solution Serum Albumin, Bovine Technique, Dilution Xylene

Zika Virus RNA Detection in Situ

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Publication 2023

Aftercare Antibodies Bath Biological Assay Buffers Cells Cytokeratin 8 Diagnosis DNA Replication Ethanol Fluorescence Genome Genomic Hybridization Hematoxylin Immunofluorescence Keratin-5 NUP214 protein, human Olfactory Marker Protein Olfactory Receptor Cells Peptide Hydrolases Peroxidase Peroxide, Hydrogen RNA Probes S100 Calcium Binding Protein beta Subunit Sense of Smell Tissues Xylene Zika Virus

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Ab53280 by Abcam

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Ab53280 is a primary antibody designed for use in immunohistochemistry applications. The antibody targets a specific protein, but no additional details about the intended use or the target protein are provided.

E cadherin by Cell Signaling Technology

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E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining the structural and functional integrity of epithelial tissues. It is a transmembrane protein that mediates homophilic interactions between neighboring cells, contributing to the formation and stability of adherens junctions.

Keratin 8 by Abcam

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Keratin 8 is a type of intermediate filament protein that is a component of the cytoskeleton in epithelial cells. It is commonly used as a marker for simple and glandular epithelial cells in immunohistochemistry and other research applications.

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The CellSearch® system is a laboratory instrument designed for the detection and enumeration of circulating tumor cells (CTCs) in blood samples. It utilizes immunomagnetic cell separation and fluorescence-based imaging techniques to identify and count specific cell populations.

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Keratin 14 by Fortrea

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What are the different variants or types of Cytokeratin 8?

Cytokeratin 8 (CK8) is a type II intermediate filament protein that exists in multiple isoforms or variants. The main isoforms include CK8.1, CK8.2, and CK8.3, which differ in their amino acid sequence and post-translational modifications. These variants can have slightly different functions and localization patterns within epithelial cells. Researchers studying CK8 should be aware of these variations and choose the appropriate isoform for their specific research questions and applications.

How can Cytokeratin 8 be used as a marker for epithelial differentiation?

Cytokeratin 8 is widely used as a marker for epithelial cell differentiation and lineage tracing. It is expressed in simple and stratified epithelial cells, and its expression patterns can provide insights into the developmental stage and lineage of these cells. Researchers can leverage CK8 immunohistochemistry or CK8 gene expression analysis to identify and characterize epithelial cell populations, which is particularly useful in studies of tissue development, regeneration, and cancer.

What are some of the key applications of Cytokeratin 8 in cancer research?

Cytokeratin 8 has been implicated in various cancer types and is often used as a biomarker for disease detection and monitoring. For example, elevated levels of CK8 have been associated with breast, prostate, and liver cancers, and it can serve as a useful prognostic indicator. Additionally, CK8 expression patterns can provide insights into the epithelial-mesenchymal transition (EMT) process, which is crucial for cancer metastasis. Researchers studying cancer biology and progression can benefit from investigating Cytokeratin 8 as a potential diagnostic and therapeutic target.

How can PubCompare.ai assist with Cytokeratin 8 research?

PubCompare.ai's inteligent platform can greatly streamline and optimize Cytokeratin 8 research. The tool allows you to efficiently screen the literature, including published articles, preprints, and patents, to identify the most relevant protocols and experimental methods related to CK8. The platfom's AI-powered analysis can then help you pinpoint critical insights, such as the most effective and reproducible techniques for CK8 detection, quantification, and functional studies. This can save researchers valuable time and effort, enabling them to focus on the most promising approaches for their specific Cytokeratin 8-related investigations.

What are some common challanges in working with Cytokeratin 8 and how can PubCompare.ai help?

One of the key challenges in working with Cytokeratin 8 is the potential variability in experimental protocols and the need to identify the most robust and reproducible methods. Researchers may struggle to locate the most up-to-date and accurate protocols from the vast body of literature. PubCompare.ai's AI-driven platform can assist by quickly surfacing the most relevant protocols and leveraging machine learning to highlight critical differences in their effectiveness. This enables researchers to confidently choose the best protocols for their Cytokeratin 8 studies, optimizing for reproducibility and accurate results. The platfom's streamlined approach helps overcome common hurdles and accelerates progress in Cytokeratin 8-related investigations.

More about "Cytokeratin 8"

Cytokeratin 8 (CK8), a type II intermediate filament protein, is a crucial component of the cytoskeleton in simple and stratified epithelial cells.
It forms heterodimers with its type I counterpart, cytokeratin 18 (CK18), to maintain the structural integrity of epithelial tissues.
This keratin is widely used as a marker for epithelial cell differentiation and is often implicated in various cancers and disease states.
Researchers can optimize their Cytokeratin 8 studies by utilizing the intelligent platform provided by PubCompare.ai.
This solution helps locate relevant protocols from literature, preprints, and patents, and leverages AI-powered comparisons to identify the most reproducible and accurate results.
This streamlined research approach offers an efficient way to advance Cytokeratin 8-related investigations.
In addition to Cytokeratin 8, other related terms and concepts include DAPI (a fluorescent dye that binds to DNA), Ab53280 (a Cytokeratin 8 antibody), E-cadherin (a cell-cell adhesion molecule), Keratin 8 (the same as Cytokeratin 8), CellSearch® system (a circulating tumor cell detection platform), Alexa Fluor 488 (a fluorescent dye), Keratin 14 (another intermediate filament protein), Alexa Fluor 647 (another fluorescent dye), Alexa Fluor 488 goat anti-mouse (a secondary antibody), and Bovine serum albumin (a common protein used in cell culture and immunoassays).
Incorporating these related terms and concepts can provide a more comprehensive understanding of Cytokeratin 8 and its applications in research and diagnostics.