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Epigen

Epigen is a field of study that focuses on the heritable changes in gene expression that occur without alterations in the DNA sequence.
This emerging area of research examines how environmental and developmental factors can influence gene regulation and lead to phenotypic variations.
Epigen research has important implications for understanding disease pathogenesis, individual responses to therapys, and the role of epigenetice modifications in cellular differentiation and tissue homeostasis.
PubCompare.ai's AI-driven platform can enhance reproducibiluty and accuracy in your Epigen research by helping you easily locate relevant protocols from literature, preprints, and patents, and leveraging AI-powered comparisons to identify the best protocols and products for your needs.
Experience seamless research optimization with their intelligent solution.

Most cited protocols related to «Epigen»

1
Genetic Ancestry Analysis of Epigen-Brazil
Cited 11 times
The Epigen-Brazil participants were genotyped by the Illumina facility (San Diego, California) using the Omni 2.5M array. We performed the unsupervised tri hybrid (k = 3) ADMIXTURE analyses based on 370,539 SNPs shared by samples from the HapMap Project, the Human Genome Diversity Project (HGDP)23 24 and the Epigen-Brazil study population. As external panels, we used the following HapMap samples: 266 Africans (176 Yoruba in Ibadan, Nigeria [YRI] and 90 Luhya in Webuye, Kenya [LWK]), 262 Europeans (174 Utah residents with Northern and Western European ancestry [CEU] and 88 from Toscans from Italy [TSI]), 170 admixed individuals (77 Mexicans from Los Angeles, California [MEX] and 83 Afro-African from Southwest USA [ASW]), and 93 Native Americans from the HGDP (25 Pima, 22 Karitiana, 25 Maya and 21 Surui). The same set of reference populations was used in analyzing the three cohorts.
Lima-Costa M.F., Rodrigues L.C., Barreto M.L., Gouveia M., Horta B.L., Mambrini J., Kehdy F.S., Pereira A., Rodrigues-Soares F., Victora C.G, & Tarazona-Santos E. (2015). Genomic ancestry and ethnoracial self-classification based on 5,871 community-dwelling Brazilians (The Epigen Initiative). Scientific Reports, 5, 9812.
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Publication 2015
African People American Indian or Alaska Native Epigen Europeans HapMap Hybrids Negroid Races Population Group Potassium Iodide Single Nucleotide Polymorphism
2
Intestinal Cell Culture Conditions
Cited 8 times
Primary IECs were cultured in a modified, hormonally defined medium with DMEM and F12 (vol 1:1) supplemented with 5% FCS (PAA), 2% glucose, 20 mM Hepes, 2 mM glutamin, 5 μg/ml insulin, 50 nM dexamethasone, 60 nM sodium selenite, 10 ng/ml epithelial growth factor, 5 μg/ml transferrin, and 1 nM 3,3′,5-triiodo-l-thyronine sodium salt as described previously (38 (link)). To prevent microbial growth, 100 U/ml penicillin, 100 μg/ml streptomycin, and 2.5 μg/ml amphotericin B (Sigma-Aldrich) were added. Cells were incubated at 37°C in a 5% CO2/95% humidity air atmosphere on collagen-coated cell culture dishes. Primary intestinal macrophages or blood monocytes were cultured in RPMI 1640 medium supplemented with 5% FCS (PAA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Invitrogen). Murine small intestinal epithelial m-ICcl2 cells were cultured as described previously (6 (link), 39 (link)). RAW 264.7 cells were obtained from the American Type Culture Collection and propagated in RPMI 1640 medium (Invitrogen) supplemented with 20 mM Hepes, 2 mM l-glutamine, and 10% FCS. Plasmid DNA was prepared using the EndoFree Plasmid kit from QIAGEN, and transfection was performed using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instructions. Stimulation was performed by the addition of 50 ng/ml TNF, 100 ng/ml IL-1β, or LPS at the indicated concentrations. Cell culture supernatants were harvested after 12 h and stored at −20°C until further analysis. For kinase inhibition, m-ICcl2 cells were preincubated for 40 min in the presence of 25 μM K-252b and subsequently stimulated with 100 ng/ml LPS for 2 h. 5 μg of total cell lysate was analyzed by Western blot for the presence of IRAK-1.
Lotz M., Gütle D., Walther S., Ménard S., Bogdan C, & Hornef M.W. (2006). Postnatal acquisition of endotoxin tolerance in intestinal epithelial cells. The Journal of Experimental Medicine, 203(4), 973-984.
Publication 2006
Amphotericin B Atmosphere Cell Culture Techniques Cells Collagen Dexamethasone Epigen Glucose Glutamine HEPES Humidity Hyperostosis, Diffuse Idiopathic Skeletal Insulin Interleukin-1 beta Intestines IRAK1 protein, human K 252b lipofectamine 2000 Macrophage M Cells M Cells, Intestinal Monocytes Mus Penicillins Phosphotransferases Plasmids Psychological Inhibition RAW 264.7 Cells Selenite, Sodium Sodium Sodium Chloride Streptomycin Thyronine Transfection Transferrin Western Blot
3
Isolation and Culture of Placental Amniotic Mesenchymal Cells
Cited 5 times

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Publication 2018
Amnion Bath BLOOD Buffers Cells Cell Survival Chorion Cold Temperature Collagenase Deoxyribonucleases Eagle Edetic Acid Enzymes Epigen Epithelial Cells Fetal Bovine Serum Hanks Balanced Salt Solution Immunocytochemistry Mesenchyma Mucolipidosis Type IV Obstetric Labor Penicillin G Pepsin A Placenta Primary Cell Culture Repeat Cesarean Section Sterility, Reproductive Streptomycin Tissue, Membrane Trypan Blue Trypsin Vimentin Woman
4
Multiplex Luminex Cytokine Profiling
Cited 5 times
The Luminex xMAP™ serum assays were performed in 96-well microplate format. Analytes were chosen based on knowledge of pathophysiology of malignancy and markers reported in the literature. Multiplex bead-based immunoassays for cytokines were purchased from Invitrogen. Other assays for proteins were developed in the Luminex Core Facility of University of Pittsburgh Hillman Cancer Center (Pittsburgh, PA, USA) according to the protocol by Luminex Corporation. The analytes were: Eotaxin-1, interferon-alpha (IFN-α), interferon-gamma (IFN-γ), interleukin (IL) 10, 12 p40, 13, 15, 17, 1α, 2, 4, 5, 6, 7, 8, interferon γ-inducible protein 10 (IP-10), monocyte chemotactic protein α (MCP-1), monocyte-induced γ interferon (MIG), macrophage inflammatory protein 1-α (MIP-1 α), macrophage inflammatory protein 1-β (MIP-1β), regulated upon activation, normally T-expressed and presumably secreted (RANTES), tumor necrosis factor alpha (TNF-α), tumor necrosis factor receptor I (TNF-RI), tumor necrosis factor receptor II (TNF-RII), death receptor 5 (DR5), epithelial growth factor (EGF), basic fibroblast growth factor (bFGF), granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), hepatocyte growth factor (HGF), and vascular endothelial growth factor (VEGF).
Internal (spiked) control analytes were included for validation and standard curves. For intra-assay precision, CVs were calculated from six replicates of a control serum run on a single plate. For inter-assay precision, CVs were calculated from at least five separate experimental runs of the same control serum. The intra-assay variability was 3.5–7%. Inter-assay variability was 11–15%. Correlation with appropriate ELISA was 89–98% and recovery from serum was 80–110%. Table 2 provides a complete list of the 32 factors originally assayed via the xMAP procedure, 19 of which met the inclusion criteria for statistical analysis (see below).
Linkov F., Ferris R.L., Yurkovetsky Z., Marrangoni A., Velikokhatnaya L., Gooding W., Nolan B., Winans M., Siegel E.R., Lokshin A, & Stack BC J.r. (2008). Multiplex analysis of cytokines as biomarkers that differentiate benign and malignant thyroid diseases. Proteomics. Clinical applications, 2(12), 1575-1585.
Publication 2008
A-factor (Streptomyces) CCL3 protein, human CCL4 protein, human CXCL10 protein, human Cytokine Enzyme-Linked Immunosorbent Assay Eotaxin-1 Epigen factor A Fibroblast Growth Factor 2 Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte Colony-Stimulating Factor Hepatocyte Growth Factor Immunoassay Interferon-alpha Interferon Type II Interleukin-10 Malignant Neoplasms Monocyte Chemoattractant Proteins Monocytes Necrosis Neoplasms Proteins Prothrombin Receptors, Tumor Necrosis Factor, Member 10b Serum TNF protein, human TNFRSF1A protein, human TNFRSF1B protein, human Vascular Endothelial Growth Factors
5
Constructing a Comprehensive Brazilian Genetic Reference Panel
Cited 4 times
We used two reference panels: (1) the public 1000 Genomes Project Phase 3 haplotypes, version 20130502, (1KGP) (Sudmant et al. 2015 (link)); and (2) The EPIGEN-5M+1KGP reference panel, which is the merge of the 1KGP panel and our unpublished EPIGEN-5M panel, bearing 14,970 more SNPs than the public panel solely. The EPIGEN-5M data set was genotyped with the Illumina HumanOmni5-4v1 array. After quality control, the data set comprises 4,102,271 SNPs for 265 Brazilians from the three cohorts (90, 88, and 87 individuals from Salvador, Bambuí, and Pelotas, respectively) (Supplemental Table S2). We used SHAPEIT2 (Delaneau et al. 2013 (link)) to infer the chromosome phase of the EPIGEN-5M data set (Supplemental Tables S4–S8).
Magalhães W.C., Araujo N.M., Leal T.P., Araujo G.S., Viriato P.J., Kehdy F.S., Costa G.N., Barreto M.L., Horta B.L., Lima-Costa M.F., Pereira A.C., Tarazona-Santos E, & Rodrigues M.R. (2018). EPIGEN-Brazil Initiative resources: a Latin American imputation panel and the Scientific Workflow. Genome Research, 28(7), 1090-1095.
Publication 2018
Chromosomes Epigen Genome Haplotypes Single Nucleotide Polymorphism

Most recents protocols related to «Epigen»

1
Culturing Prostate Cell Lines
All PCa cell lines were cultured in RPMI-1640 medium with a Glutamax supplement (Life Technologies) and supplemented with 10% fetal bovine serum (FBS) and an antibiotic mixture of 1× penicillin/streptomycin obtained from Life Technologies and LabClinics, respectively. RWPE1 cells were cultured in Keratinocyte serum free medium supplemented with bovine pituitary extract (BPE) and epithelial growth factor (EGF), obtained from Life Technologies. Cells were seeded in Nunclon™ (Nunc) Delta surface treated 6-well plates and 8-well chamber slides (Lab Tek) obtained from Thermosfisher for flow cytometry analysis and confocal imaging, respectively.
Murar M., Pujals S, & Albertazzi L. (2023). Multivalent effect of peptide functionalized polymeric nanoparticles towards selective prostate cancer targeting. Nanoscale Advances, 5(5), 1378-1385.
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Publication 2023
Bos taurus Cell Lines Cells Culture Media Epigen Fetal Bovine Serum Flow Cytometry Keratinocyte Penicillins Serum Streptomycin
2
Genetic Analysis of FLG Variants

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Publication 2023
BLOOD Child Chromosomes, Human, Pair 1 Epigen Grandparent Phenotype Population Group Reproduction Single Nucleotide Polymorphism X Chromosome
3
Breast Cancer Tumorsphere Formation Assay
To measure tumorsphere formation [26 (link)] BT474 (1 × 104 cells/well), SKBR3 (5 × 103 cells/well), and HCC-1954 (5 × 103 cells/well) cells were plated in DMEM-Gluta MAX medium (Invitrogen) containing 2% B27 (Invitrogen), 4 ng/mL heparin (Sigma-Aldrich), 20 ng/mL epithelial growth factor (Sigma-Aldrich), and 20 ng/mL basic fibroblast growth factor (PeproTech, Rocky Hill, NJ, USA) in 6-well ultra-low attachment surface plates (Corning, Corning, NY, USA). JIMT-1 (1 × 104 cells/well) cells were similarly seeded in DMEM-Gluta MAX medium supplemented with 2% B27, 4 ng/mL heparin, 20 ng/mL EGF, 20 ng/mL bFGF, 5 mg/mL insulin, and 0.5 mg/mL hydrocortisone (Sigma-Aldrich), in ultra-low attachment surface plates. An inverted microscope was used to assess and quantify tumorsphere formation after 5 days.
Moon S.J., Choi H.J., Kye Y.H., Jeong G.Y., Kim H.Y., Myung J.K, & Kong G. (2023). CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer. Cancers, 15(4), 1168.
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Publication 2023
Cells Epigen Fibroblast Growth Factor 2 Heparin Hydrocortisone Insulin Microscopy
4
Isolation of Amniotic Membrane Cells
Fetal membranes were washed with ice-cold phosphate-buffered saline (PBS, Corning, NY, USA) with 1% penicillin–streptomycin solution (10,000 U/mL penicillin, 10,000 U/mL streptomycin, Corning, Steuben County, NY, USA). The amniotic membrane was mechanically peeled off the underlying chorion layer to remove any blood clots. Then the tissues were incubated for 10 min at room temperature with PBS/ethylenediaminetetraacetic acid (EDTA) 0.5 mM. The amniotic membrane was then minced into small pieces (4 cm2 approximately) and digested twice for 30 min at 37 °C using trypsin-EDTA 0.25% (Corning, Steuben County, NY, USA) with gentle shaking. For both digestion steps, trypsin was inactivated with fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, USA) and the cell suspension was centrifuged for 10 min at 390× g. The cell pellet was resuspended in basal culture medium, Dulbecco’s Modified Eagle’s Medium–high glucose (DMEM H., Corning, Steuben County, NY, USA) containing 10% FBS, 1% penicillin-streptomycin solution, and 10 ng/mL of epithelial growth factor (EGF, Sigma-Aldrich, St. Louis, MO, USA). The single-cell suspension was counted and tested for viability using erythrosin B (Sigma-Aldrich, St. Louis, MO, USA). Only samples with >90% viability were used for further assays.
Paris F., Marrazzo P., Pizzuti V., Marchionni C., Rossi M., Michelotti M., Petrovic B., Ciani E., Simonazzi G., Pession A., Bonsi L, & Alviano F. (2023). Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy. Bioengineering, 10(2), 189.
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Publication 2023
Amnion Biological Assay Cells Chorion Cold Temperature Digestion Eagle Edetic Acid Epigen Erythrosine Fetal Membranes Glucose Penicillins Phosphates Saline Solution Streptomycin Thrombus Tissues Trypsin
5
Cultivating and Treating Human Respiratory Cells
Normal human bronchial epithelial cells (NHBE, Lonza, Basel, Switzerland) were purchased from Lonza (Cat. No. CC-2450) and were maintained in Keratinocyte SFM basal medium supplement with 5 μg/L human recombinant epithelial growth factor (EGF), 50 mg/L Bovine Pituitary Extract (BPE), 5 mg/L insulin and 25 nM hydrocortisol. Cells were passaged every 3 days and plated for experiment treatment within 6 passages. Human alveolar type II epithelia cells A549 cell was purchased from ATCC (Cat.No.CCL-185) and were maintained in DMEM medium supplement with 10% FBS, sodium pyruvate, and 1% penicillin/streptomycin. Cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. CuSO4, chloroquine, N-acetylcysteine, fusin, apigenin, kaempferol, and genkwanin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Chiou H.Y., Wang C.W., Chen S.C., Tsai M.L., Lin M.H., Hung C.H, & Kuo C.H. (2023). Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human. Antioxidants, 12(2), 532.
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Publication 2023
A549 Cells Acetylcysteine Apigenin Atmosphere Bos taurus Bronchi Cells Chloroquine CXCR4 Receptors Dietary Supplements Epigen Epithelial Cells Epithelium genkwanin Homo sapiens Insulin kaempferol Keratinocyte Penicillins Pyruvate Sodium Streptomycin Therapies, Investigational Type-II Pneumocytes

Top products related to «Epigen»

1
Epithelial growth factor (egf) by Thermo Fisher Scientific
Sourced in United States
Epithelial growth factor is a protein that stimulates the growth and differentiation of epithelial cells. It plays a key role in the repair and regeneration of epithelial tissues.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Hydrocortisone by Merck Group
Sourced in United States, Germany, United Kingdom, France, China, Italy, Canada, Macao, Japan, Israel, Switzerland, Australia, Sao Tome and Principe, Spain, Austria, Portugal, Belgium, Denmark, Sweden, Argentina, Brazil, Poland, New Zealand
Hydrocortisone is a laboratory-grade reagent used in various research and analytical applications. It is a synthetic corticosteroid compound with anti-inflammatory and immunosuppressant properties. Hydrocortisone is commonly utilized as a standard or reference material in analytical procedures, such as assays and chromatographic techniques, to quantify and identify related compounds.
4
Dmem f12 by Thermo Fisher Scientific
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DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.
5
Insulin by Merck Group
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Insulin is a lab equipment product designed to measure and analyze insulin levels. It provides accurate and reliable results for research and diagnostic purposes.
6
Basic fibroblast growth factor (bfgf) by Thermo Fisher Scientific
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The BFGF is a laboratory instrument designed for the controlled growth and expansion of cells. It provides a regulated and consistent environment for cell culture applications. The core function of the BFGF is to maintain optimal temperature, humidity, and gas composition to support the proliferation and differentiation of cells.
7
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
8
N2 supplement by Thermo Fisher Scientific
Sourced in United States, Germany, Japan, United Kingdom, France, Italy, China, Canada, Czechia, Belgium, Australia, Switzerland
The N2 supplement is a laboratory-grade nitrogen enrichment solution used to support the growth and development of cell cultures. It provides an additional source of nitrogen to cell culture media, which is essential for cellular metabolism and protein synthesis.
9
Epithelial growth factor (egf) by Merck Group
Sourced in United States, Germany
Epithelial growth factor is a laboratory reagent used in cell culture applications. It is a protein that stimulates the growth and proliferation of epithelial cells. The core function of epithelial growth factor is to promote cell division and differentiation in epithelial cell lines.
10
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

More about "Epigen"

Epigenetics is a rapidly evolving field of study that focuses on the heritable changes in gene expression without alterations in the DNA sequence.
This emerging area of research, also known as Epigen, examines how environmental and developmental factors can influence gene regulation and lead to phenotypic variations.
Epigenetic modifications, such as DNA methylation, histone modifications, and chromatin remodeling, play a crucial role in cellular differentiation, tissue homeostasis, and disease pathogenesis.
Understanding these epigenetic mechanisms has important implications for personalized medicine, as it can help explain individual responses to therapies and guide the development of novel treatments.
Epigen research often utilizes various cell culture techniques and supplements, including epithelial growth factor (EGF), fetal bovine serum (FBS), hydrocortisone, DMEM/F12 media, insulin, basic fibroblast growth factor (bFGF), penicillin/streptomycin, and N2 supplement.
These components can influence cellular processes and gene expression, making them important considerations in epigenetic studies.
To enhance the reproducibility and accuracy of your Epigen research, PubCompare.ai's AI-driven platform can be a valuable tool.
Their solution helps you easily locate relevant protocols from literature, preprints, and patents, and leverages AI-powered comparisons to identify the best protocols and products for your needs.
Experience seamless research optimization with their intelligent solution and take your Epigen studies to the next level.