The online version of RNAhybrid is an easy-to-use web interface in which the user can upload his or her own miRNA and candidate target sequences. A number of options give broad control over the kind of interaction the program looks for. A prevailing assumption about functional miRNA/target interactions is the necessity of a ‘seed’ (6 (link)), a perfect Watson–Crick match between miRNA and target at miRNA positions 2–7 or 8. However, experimentally validated miRNA/target duplexes in Caenorhabditis elegans appear to have unpaired nucleotides in this very seed region (18 (link)). In (11 (link)), it was experimentally shown that a target site with a seed region as small as only 4 nt can be functional as long as there is a compensatory hybridization at the miRNA 3′ end. RNAhybrid answers this heterogeneity by allowing the user to freely choose the (algorithmic) necessity and nature of a seed. First, the position and length of the seed can be defined; second, G:U wobble base pairs with the seed may be allowed or not and third, the request for a seed in the prediction can be refrained from altogether. The disallowance of G:U pairs in the seed is one of the new features and has been requested frequently. Another novelty is a ‘seed-match speed-up’, in which in an initial filter step, candidate targets are searched for seed matches, only upon finding such matches the complete hybridization around the seed-match is calculated. For non-G:U seeds of length 6, this implements a speed-up of a factor of 8. Another new option is to restrict possible sizes of unpaired regions, the loops. Both ‘bulge loops’, those with unpaired nucleotides on only one side, and ‘internal loops’, those with unpaired nucleotides on both sides, can be restricted in their length to user-defined values. This is especially useful in the prediction of plant miRNA targets. These targets usually exhibit only a small number of unpaired nucleotides, if any (8 (link)). Restricting loop sizes to, for example 1 nt, avoids the generation of spurious hits that do not conform to established miRNA/target hybridization rules in plants. Two other useful options are the number of target sites per miRNA and target candidate the program looks for, and a threshold for the minimum free energy of the hybridization, only below which target sites are reported. This latter option is the only option that is offered by Diana microT (15 (link)), in turn the only method besides RNAhybrid that is available for online miRNA target prediction in animals. The program miRU (19 (link)) is available as an online tool, but is geared towards prediction of potential targets in plants.
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Amino Acid
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Factor VIII
Factor VIII
Factor VIII is a critical blood coagulation protein that plays a vital role in the hemostatic process.
Also known as antihemophilic factor, this large glycoprotein is essential for normal blood clotting and is deficient in individuals with the genetic disorder hemophilia A.
PubCompare.ai's AI-driven protocol comparison tool can help researchers optimize Factor VIII studies, locating the best experimental protocols from literature, preprints, and patents to ensure reproducible and accurate findings.
Unlock the secrets of this important factor and experince the future of research today with PubCompare's intuitive platform.
Also known as antihemophilic factor, this large glycoprotein is essential for normal blood clotting and is deficient in individuals with the genetic disorder hemophilia A.
PubCompare.ai's AI-driven protocol comparison tool can help researchers optimize Factor VIII studies, locating the best experimental protocols from literature, preprints, and patents to ensure reproducible and accurate findings.
Unlock the secrets of this important factor and experince the future of research today with PubCompare's intuitive platform.
Most cited protocols related to «Factor VIII»
A-factor (Streptomyces)
Animals
Caenorhabditis elegans
Crossbreeding
factor A
Factor VIII
Genetic Heterogeneity
MicroRNAs
Nucleotides
Plant Embryos
Plants
beta-Globins
Biological Assay
Diagnosis
Escherichia coli
Factor VIII
Fluorescence
Genes
Genome
HeLa Cells
Homo sapiens
Malignant Neoplasms
Oligonucleotide Primers
Platinum
Real-Time Polymerase Chain Reaction
Solon
Sulfoxide, Dimethyl
Taq Polymerase
Telomere
Tromethamine
Alcohols
Cannabis sativa
Factor VIII
Motivation
Paranoia
Physical Examination
Reading Frames
Self-Perception
Withdrawal Symptoms
Young Adult
Affective Symptoms
Antidepressive Agents
Antipsychotic Agents
Aripiprazole
Attention
Auditory Perception
Barakat syndrome
Biopharmaceuticals
Bipolar Disorder
BLOOD
Bupropion
Central Nervous System Stimulants
Clonazepam
Cognition
Depression, Bipolar
Diagnosis
Divalproex Sodium
Emotions
Face
factor A
Factor VIII
Fingers
Genes, vif
Hospitalization
Inpatient
Lamotrigine
Lithium
Mania
Manic Episode
Memory
Mood
Neuropsychological Tests
Pharmaceutical Preparations
Phenotype
Psychological Inhibition
Psychotic Disorders
Quetiapine
Risperidone
Schizoaffective Disorder
Sedatives
Stroop Test
Tests, Diagnostic
Thyroid Gland
Thyroxine
Tranquilizing Agents
VP-P protocol
Adult
Blood Coagulation Disorders
Child
Chiroptera
Coagulants
Factor VIII
Factor VIII-Related Antigen
Females
Limulus clotting factor C
Males
Patients
Phenotype
Ristocetin
Most recents protocols related to «Factor VIII»
Protocol full text hidden due to copyright restrictions
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Actins
Biological Assay
Chromogenic Substrates
Factor VIII
Freezing
Hemophilia A
Patients
Plasma
Protocol full text hidden due to copyright restrictions
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Activated Partial Thromboplastin Time
Ellagic Acid
Factor VIII
Plasma
Silicon Dioxide
Protocol full text hidden due to copyright restrictions
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antihemophilic factor, human recombinant residues 743-1636 deleted
BAX 855
Factor VIII
Gifts
Hemophilia A
Plasma
Polyethylene Glycols
rurioctocog alfa pegol
Technique, Dilution
turoctocog alfa
Using the biomass, carotenoids content and viable yeast count of SRY, eight factors were selected as the initial pH value (A), soybean oil content (B), glucose content (C), hydrogen peroxide content (D), vitamin B1 content (E), manganese ion content (F), magnesium ion content (G) and zinc ion content (H) for Plackett-Burman test. The range of optimal culture conditions for the 8 factors was taken at high (+1) and low (−1) levels, respectively, and the PB test design factors and levels are shown in Supplementary Table 2 .
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Carotenoids
Factor VIII
Glucose
Magnesium
Manganese
Peroxide, Hydrogen
Soybean oil
Thiamine
Yeast, Dried
Zinc
MR measurements were acquired using a 9.4 T Bruker Biospec 94/20 small animal scanner equipped with a 720 mT/m gradient system (Bruker BioSpin GmbH, Ettlingen, Germany) and different combinations of transmit coils and receive-only coils. First, an anatomical image was acquired to identify the slice position for fMRI measurements: 2D Turbo spin echo sequence (RARE), TR/TEEff = 2,000/50 ms, RARE factor 8, Matrix 256 × 256, field of view (FOV) 28 mm × 26 mm, slice thickness 1.2 mm, 9–12 contiguous slices. Subsequently, B0 homogenization was performed using the MAPSHIM Bruker routine. Functional GE-EPI and SE-EPI measurements were performed using a single-shot EPI sequence (same FOV as anatomy, Matrix: 80 × 80, 1.2 mm slice thickness; GE-EPI: TE 18 ms, TR 100/125/1,000 ms, flip angle 18/21/60 or 65°, 1/2/9 or 12 slices; SE-EPI: TE 35.9 ms, TR 250 ms, flip angle 90°, 3 slices). One fMRI measurement lasted between 5 and 25 min (Supplementary Table 1 ).
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Animals
ECHO protocol
Factor VIII
fMRI
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Factor VIII is a laboratory reagent used in coagulation testing. It is a protein that plays a crucial role in the blood clotting process. The core function of Factor VIII is to facilitate the activation of Factor X, which is an essential step in the formation of a stable blood clot.
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