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GLB1 protein, human
GLB1 protein, human
The GLB1 gene encodes the beta-galactosidase enzyme, which plays a crucial role in the lyosomal degradation of glycoconjugates.
Mutations in GLB1 can lead to the rare genetic disorder galactosidosis, also known as GM1 gangliosidosis.
Researchers studying the GLB1 protein can use PubCompare.ai's AI-powered platform to optimize their workflow by exploring protocols from literature, preprints, and patents, and leveraging AI-driven comparisons to identify the best protocols and products for their specific research needs.
With PubCompare.ai's advanced features, scientists can streamline their research and accelerate their discoveries related to the GLB1 protien.
Mutations in GLB1 can lead to the rare genetic disorder galactosidosis, also known as GM1 gangliosidosis.
Researchers studying the GLB1 protein can use PubCompare.ai's AI-powered platform to optimize their workflow by exploring protocols from literature, preprints, and patents, and leveraging AI-driven comparisons to identify the best protocols and products for their specific research needs.
With PubCompare.ai's advanced features, scientists can streamline their research and accelerate their discoveries related to the GLB1 protien.
Most cited protocols related to «GLB1 protein, human»
For the influenza hemagglutinin trimer benchmark, raw movie data and pre-extracted particles from EMPIAR-10097 were downloaded. The movies were processed with the full Warp pipeline using the following settings: motion correction with a temporal resolution of 40 for the global motion, and 5x5 spatial resolution for the local motion, using the 0.03–0.25 Nyquist range and a B-factor of -400 A2; CTF estimation with 6x6 spatial resolution, using the 0.1–0.35 Nyquist range; particle picking with a BoxNet model retrained on particles from 3 micrographs, using the default 0.95 threshold. Quality filters were applied in Warp as follows: defocus between 0.3 and 5.0 µm, resolution better than 8 Å, intra-frame motion of at most 1.5 Å, particle count above 120. Particles were extracted from the micrographs meeting these filters and subjected to processing in cryoSPARC: no 2D classification was performed; ab initio refinement was performed with 6 classes and no symmetry; the 6 classes were then refined heterogeneously, with no symmetry imposed; the only class showing the expected Hemagglutinin structure was refined with C3 symmetry. The original particle set from EMPIAR-10097 was subjected to 3 different processing strategies. First, the full set was refined in cryoSPARC with C3 symmetry using the original CTF estimates. Second, the full set was subjected to the same classification and refinement as the particles from Warp, using the original CTF estimates. Third, particles from the Hemagglutinin class obtained in the second processing branch were updated with local CTF estimates from Warp, and refined again with C3 symmetry. Resolution estimates were obtained for all maps using the respective masks automatically generated by cryoSPARC.
For the β-galactosidase benchmarking studies, raw data from EMPIAR-10061 were downloaded. The movies were processed with the full Warp pipeline using the following settings: motion correction with a temporal resolution of 38 for the global motion, and a 5x5 spatial resolution for the local motion, using the 0.03–0.60 Nyquist range and a B-factor of -160 Å2; CTF estimation with 5x5 spatial resolution, using the 0.08–0.60 Nyquist range; particle picking with a BoxNet model retrained on particles from 5 micrographs, using a threshold of 0.30. No quality filters were used as the data already represent a high-quality subset curated for the initial publication. Picked and extracted particles were subjected to 2D and 3D classification with C1 symmetry in RELION 2.1 to remove incomplete particles. The remaining particles were refined with D2 symmetry. The final half-maps were then used to refine beam tilt and per-particle defocus in RELION 3.0. Global motion tracks for all movies were exported from Warp to RELION 3.0 to perform Bayesian particle polishing.
To assess the frame alignment accuracy in Warp independently of downstream map refinement, β-galactosidase movies were aligned in Warp as described above, and using the default settings in MotionCor2. CTF fitting was performed with 5x5 spatial resolution, using the 0.08–50 Nyquist range. Frequency-dependent fit quality was calculated as described in the ‘Resolution estimation’ section, and all resulting curves averaged. The resolution was then estimated at a cut-off value of 0.3.
For the β-galactosidase benchmarking studies, raw data from EMPIAR-10061 were downloaded. The movies were processed with the full Warp pipeline using the following settings: motion correction with a temporal resolution of 38 for the global motion, and a 5x5 spatial resolution for the local motion, using the 0.03–0.60 Nyquist range and a B-factor of -160 Å2; CTF estimation with 5x5 spatial resolution, using the 0.08–0.60 Nyquist range; particle picking with a BoxNet model retrained on particles from 5 micrographs, using a threshold of 0.30. No quality filters were used as the data already represent a high-quality subset curated for the initial publication. Picked and extracted particles were subjected to 2D and 3D classification with C1 symmetry in RELION 2.1 to remove incomplete particles. The remaining particles were refined with D2 symmetry. The final half-maps were then used to refine beam tilt and per-particle defocus in RELION 3.0. Global motion tracks for all movies were exported from Warp to RELION 3.0 to perform Bayesian particle polishing.
To assess the frame alignment accuracy in Warp independently of downstream map refinement, β-galactosidase movies were aligned in Warp as described above, and using the default settings in MotionCor2. CTF fitting was performed with 5x5 spatial resolution, using the 0.08–50 Nyquist range. Frequency-dependent fit quality was calculated as described in the ‘Resolution estimation’ section, and all resulting curves averaged. The resolution was then estimated at a cut-off value of 0.3.
Complement Factor B
Eye Movements
GLB1 protein, human
Hemagglutinin
Microtubule-Associated Proteins
Reading Frames
Virus Vaccine, Influenza
Optical fiber bundles were purchased from Schott North America (Southbridge, MA). Non-reinforced gloss silicone sheeting was obtained from Specialty Manufacturing (Saginaw, MI). Hydrochloric acid, anhydrous ethanol, and molecular biology grade Tween-20 were all from Sigma-Aldrich (Saint Louis, MO). 2.7-μm-diam. carboxyl-terminated magnetic beads were purchased from Varian, Inc. (Lake Forest, CA). Monoclonal anti-human TNF-α capture antibody, polyclonal anti-human TNF-α detection antibody, and recombinant human TNF-α were purchased from R&D Systems (Minneapolis, MN). Monoclonal anti-PSA capture antibody, monoclonal anti-PSA detection antibody, and purified PSA were purchased from BiosPacific (Emeryville, CA); the detection antibody was biotinylated using standard methods. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (NHS), and SuperBlock® T-20 Blocking Buffer were purchased from Thermo Scientific (Rockford, IL). Purified DNA was purchased from Integrated DNA Technologies (Coralville, IA). Streptavidin-β-galactosidase (SβG) was conjugated in house using standard protocols. Resorufin-β-D-galactopyranoside (RGP) was purchased from Invitrogen (Carlsbad, CA). The fiber polisher and polishing consumables were purchased from Allied High Tech Products (Rancho Dominguez, CA).
Absolute Alcohol
Antibodies, Anti-Idiotypic
Buffers
Carbodiimides
Etanercept
Fibrosis
Forests
GLB1 protein, human
Homo sapiens
Hydrochloric acid
Immunoglobulins
Monoclonal Antibodies
N-hydroxysulfosuccinimide
resorufin galactopyranoside
Silicones
Streptavidin
TNF protein, human
Tween 20
Carbon
casamino acids
Escherichia coli K12
Gene Expression
GLB1 protein, human
Glucose
Glycerin
Glycine
Nitrogen
Oligonucleotides
Proteins
Strains
Tetracycline
We selected regions containing cCRE-dELSs in three E11.5 mouse tissues (midbrain, hindbrain, and limb) for testing using E11.5 transgenic mouse assays. We excluded dELS-containing regions that overlapped any previously tested regions that were already in the VISTA database (http://enhancer.lbl.gov/ ). We ranked dELS-containing regions from the most to the least significant by the average rank of DNase and H3K27ac signals in the corresponding tissue and then selected regions from three segments of each tissue’s ranked list (the top, around 1,500, and around 3,000 by rank). We used H3K27ac peaks (called using the ENCODE uniform processing pipeline) that overlapped the cCRE-dELSs to choose the boundaries of the tested regions. In total, we tested 151 regions across the three tissues (Supplementary Table 22 ).
Transgenic mouse assays were performed in FVB/NCrl strain M. musculus animals (Charles River) as described previously49 (link). In brief, predicted enhancers were PCR amplified and cloned into a plasmid upstream of a minimal Hsp68 promoter and a lacZ reporter gene. The plasmids were pronuclear injected into fertilized mouse eggs, and the transgenic embryos were implanted into surrogate mothers, collected at E11.5, and stained for β-galactosidase activity. A predicted element was scored positive as an enhancer if at least three embryos had identical β-galactosidase staining in the same tissue. Conversely, a prediction was deemed inactive if no reproducible staining was observed and at least five embryos harbouring a transgene insertion were obtained.
Transgenic mouse assays were performed in FVB/NCrl strain M. musculus animals (Charles River) as described previously49 (link). In brief, predicted enhancers were PCR amplified and cloned into a plasmid upstream of a minimal Hsp68 promoter and a lacZ reporter gene. The plasmids were pronuclear injected into fertilized mouse eggs, and the transgenic embryos were implanted into surrogate mothers, collected at E11.5, and stained for β-galactosidase activity. A predicted element was scored positive as an enhancer if at least three embryos had identical β-galactosidase staining in the same tissue. Conversely, a prediction was deemed inactive if no reproducible staining was observed and at least five embryos harbouring a transgene insertion were obtained.
Animals
Animals, Transgenic
Biological Assay
Deoxyribonuclease I
Embryo
GLB1 protein, human
Hindbrain
LacZ Genes
Mesencephalon
Mice, Laboratory
Mice, Transgenic
Plasmids
Rivers
Strains
Surrogate Mothers
Tissues
Transgenes
Zygote
Most recents protocols related to «GLB1 protein, human»
The DNA fragments for Brf1 gene promoter 4, Gtf3C2 gene promoter 2, and MDM2 gene promoter 1 were amplified from 293T genomic DNA by PCR. The resulting promoter DNA was inserted into pGL3-basic reporter vector (Promega). 293T and HeLa cells were cultured in 12-well plates for 24 h and were subjected to transient transfection with the Brf1P4 or Gtf3c2P2-driving reporter vectors and the vectors expressing β-galactosidase, where three biological replicates for treatment or control groups were designed. After 48 h, cell samples were harvested and lysed with 100 μl of lysis buffer provided by a dual-light luciferase detection kit (Promega). Five microliters of cell lysate was used to detect the activities of luciferase and β-galactosidase based on the manual provided by the kit. The luciferase activity of each sample was normalized by the activity of β-galactosidase within the same sample. Relative luciferase activity was obtained by comparing the luciferase activity from the treatment samples to that from the control samples, where the activity of the control sample was arbitrarily set as 1. For reporter assays using MDM2P1-driving reporter vectors, luciferase activity for treatment and control was normalized by the protein quantity used in each assay.
Biological Assay
Biopharmaceuticals
Buffers
Cells
Cloning Vectors
Genome
GLB1 protein, human
HeLa Cells
Light
Luciferases
MDM2 protein, human
Paragangliomas 3
Promega
Promoter, Genetic
Proteins
Specimen Handling
Transfection
Transients
To examine the interaction among RdFV CP, HongrES1, and RGDV P8, the DUALmembrane starter kit (Dualsystems Biotech, P01201-P01229) was used according to the manufacturer’s instructions. The full-length ORFs of RdFV CP and RGDV P8 were separately inserted into bait vector pDHB1 to generate pDHB1-CP and pDHB1-P8, and the full-length ORFs of HongrES1, RdFV CP, RSMV G, and RDV P8 were separately inserted into prey vector pPR3-N to generate pPR3-N-HongrES1, pPR3-N-CP, pPR3-N-G and pPR3-N-P8 (RDV). The bait and prey were co-transformed the yeast strain NMY51. The pLargeT/p53 interaction served as a positive control, and the pDHB1/pRR3N served as a negative control. Transformants were subsequently screened on the QDO (SD/-Trp-Leu-His-Ade/X-Gal) culture medium, and β-galactosidase activity was detected in Z buffer with X-Gal. The primers used in Y2H were shown in Supplementary Table 1 .
5-bromo-4-chloro-3-indolyl beta-galactoside
Buffers
Cloning Vectors
Culture Media
GLB1 protein, human
Oligonucleotide Primers
Open Reading Frames
Photoparoxysmal Response 3
Strains
tryptophan-leucine
Yeast, Dried
The Materials and methods are described in Supplementary material , Materials and Methods. They include bacterial strains, growth conditions, qRT-PCR, β-galactosidase assay, purification of recombinant SqrR and SQR, sulfurtransferase assay, fluorescence anisotropy and mass spectrometry-based sulfuromics analysis. All primers used in this research are listed in Table S4 . Data represent the mean of at least three independent experiments (error bars indicate SE of the mean). The P-value and statistical significance of difference were analyzed by using unpaired t-tests (P < 0.05, significant).
Anisotropy, Fluorescence
Bacteria
Biological Assay
GLB1 protein, human
Growth Disorders
Mass Spectrometry
Oligonucleotide Primers
Strains
Sulfurtransferase
The hUC-MSCs from the PLL-PLGA scaffold with a density of 1 × 105 cells/well (100 μL) were seeded for β-Galactosidase staining analysis. As well, native hUC-MSCs with the same density from Wharton’s jelly were used as a control. Briefly, the hUC-MSCs of two groups were left to be serially cultured in an incubator at 37 °C with 5% CO2. After 10 days, the cells from different groups were collected and then, respectively, cultured in a 6-well plate with the same density (2 × 104 cells/well, 500 μL). When confluence reached 100%, the hUC-MSCs in each group were treated with a β-galactosidase kit according to the manufacturer’s instructions. After that, all samples were observed by a fluorescence microscope to assess the cell senescence.
Cells
Cellular Senescence
GLB1 protein, human
Microscopy, Fluorescence
Polylactic Acid-Polyglycolic Acid Copolymer
Wharton Jelly
The carboxyl-terminated poly lactic-co-glycolic acid (PLGA) was purchased from Meilunbio (Dalian, China). Polylysine (PLL), 4-dimethylaminopyridine (DMAP), and 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDC) were all purchased from Sigma-Aldrich (Guangzhou, China). Cell counting kit-8 (CCK-8) and senescence β-galactosidase staining kit were obtained from Beyotime Biotechnology (Shanghai, China). Human stem cell pluripotency detection kit was purchased from ScienCell (Shanghai, China). A human MSC analysis kit (562245) for flow cytometry assay was obtained from BD Biosciences (Shanghai, China). The reagents for real-time PCR were obtained from Vazyme Biotech Co., Ltd (Nanjing, China). DMEM/F12 medium was purchased from Weijia Biotechnology (Guangzhou, China).
4-dimethylaminopyridine
Biological Assay
Carbodiimides
Flow Cytometry
GLB1 protein, human
glycolic acid
Homo sapiens
Poly A
Polylysine
Real-Time Polymerase Chain Reaction
Stem Cells
WeiJia
Top products related to «GLB1 protein, human»
Sourced in United States, Japan, United Kingdom, Germany
The Senescence β-Galactosidase Staining Kit is a laboratory tool used to detect and quantify senescent cells. The kit provides reagents and protocols for the histochemical detection of senescence-associated β-galactosidase activity, a widely used biomarker for cellular senescence.
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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The Luciferase Assay System is a laboratory tool designed to measure the activity of the luciferase enzyme. Luciferase is an enzyme that catalyzes a bioluminescent reaction, producing light. The Luciferase Assay System provides the necessary reagents to quantify the level of luciferase activity in samples, enabling researchers to study biological processes and gene expression.
Sourced in China
The Senescence β-Galactosidase Staining Kit is a laboratory tool used to detect and quantify senescent cells in a sample. The kit provides the necessary reagents to perform a histochemical staining procedure that identifies the presence of the senescence-associated β-galactosidase enzyme, a widely used biomarker for cellular senescence.
Sourced in United States, China, Germany, Japan, Australia, United Kingdom
The Luciferase Assay Kit is a lab equipment product that measures the activity of luciferase enzymes. It provides the necessary reagents to quantify the amount of light produced by the luciferase-catalyzed reaction.
Sourced in United States, Switzerland
The β-Galactosidase Enzyme Assay System is a laboratory equipment used to measure the activity of the enzyme β-galactosidase. The assay system provides a colorimetric method for quantifying β-galactosidase enzyme levels in samples.
Sourced in United States, United Kingdom, Germany, France, Italy
The Reporter Lysis Buffer is a solution designed to lyse cells and release reporter proteins, such as luciferase, for analysis. It is a core component in a variety of reporter gene assays.
Sourced in United States
The β-galactosidase staining kit is a laboratory tool used to detect and visualize the presence of the β-galactosidase enzyme in cells or tissues. The kit contains the necessary reagents and protocols to perform this staining procedure.
Sourced in United States, China, Germany, Japan, United Kingdom, France, Canada, Italy, Australia, Switzerland, Denmark, Spain, Singapore, Belgium, Lithuania, Israel, Sweden, Austria, Moldova, Republic of, Greece, Azerbaijan, Finland
Lipofectamine 3000 is a transfection reagent used for the efficient delivery of nucleic acids, such as plasmid DNA, siRNA, and mRNA, into a variety of mammalian cell types. It facilitates the entry of these molecules into the cells, enabling their expression or silencing.
Sourced in United States
Mouse anti-β-galactosidase is a primary antibody that recognizes the β-galactosidase protein, which is commonly used as a reporter gene in various biological research applications.
More about "GLB1 protein, human"
The GLB1 gene encodes the beta-galactosidase enzyme, which plays a critical role in the lysosomal degradation of glycoconjugates.
Mutations in the GLB1 gene can lead to the rare genetic disorder galactosidosis, also known as GM1 gangliosidosis.
Researchers studying the GLB1 protein can leverage various tools and techniques to further their investigations.
One such tool is the Senescence β-Galactosidase Staining Kit, which can be used to detect senescent cells by measuring the activity of the beta-galactosidase enzyme.
Additionally, the Lipofectamine 2000 and Lipofectamine 3000 reagents are commonly used for transfecting cells with plasmid DNA or siRNA, allowing for the overexpression or knockdown of the GLB1 gene, respectively.
The Luciferase Assay System and Luciferase assay kit can be employed to measure the activity of the beta-galactosidase enzyme, which is often used as a reporter gene to study gene expression and regulation.
The β-Galactosidase Enzyme Assay System and Reporter lysis buffer can also be utilized for similar purposes.
Furthermore, the β-galactosidase staining kit and Mouse anti-β-galactosidase antibody can be used to visualize and quantify the expression of the beta-galactosidase protein in cells and tissues.
By leveraging these tools and techniques, researchers can optimzie their workflow, streamline their research, and accelerate their discoveries related to the GLB1 protein and its role in health and disease.
Mutations in the GLB1 gene can lead to the rare genetic disorder galactosidosis, also known as GM1 gangliosidosis.
Researchers studying the GLB1 protein can leverage various tools and techniques to further their investigations.
One such tool is the Senescence β-Galactosidase Staining Kit, which can be used to detect senescent cells by measuring the activity of the beta-galactosidase enzyme.
Additionally, the Lipofectamine 2000 and Lipofectamine 3000 reagents are commonly used for transfecting cells with plasmid DNA or siRNA, allowing for the overexpression or knockdown of the GLB1 gene, respectively.
The Luciferase Assay System and Luciferase assay kit can be employed to measure the activity of the beta-galactosidase enzyme, which is often used as a reporter gene to study gene expression and regulation.
The β-Galactosidase Enzyme Assay System and Reporter lysis buffer can also be utilized for similar purposes.
Furthermore, the β-galactosidase staining kit and Mouse anti-β-galactosidase antibody can be used to visualize and quantify the expression of the beta-galactosidase protein in cells and tissues.
By leveraging these tools and techniques, researchers can optimzie their workflow, streamline their research, and accelerate their discoveries related to the GLB1 protein and its role in health and disease.