Glucagon-Like Peptide 1
GLP-1 stimulates insulin secretion, suppresses glucagon release, and slows gastric emptying, leading to improved glycemic control.
It has also been implicated in appetite regulation and weight management.
GLP-1-based therapies, such as GLP-1 receptor agonists and dipeptidyl peptidase-4 (DPP-4) inhibitors, have emerged as effective treatments for type 2 diabbetes.
Understanding the mechanisms and functions of GLP-1 is crucial for developing new strategies to manage metabolic disorders.
Most cited protocols related to «Glucagon-Like Peptide 1»
Outcomes of interest by outcome groups in the LCS evidence-map database
Outcome groups | Outcomes of interest |
---|---|
Appetite | Appetite ratings using a visual analog scale (VAS), hunger, desire to eat, fullness, prospective consumption, thirst, motivational and behavioral factors reported through questionnaire |
Energy sensing by brain | Neurological measurements (fMRI, EEG), sensory rating (sweetness, intensity, pleasantness, sensory specific satiation), taste, perception and preference, taste reaction time |
Body weight or body composition | Body weight, body composition, BMI, waist circumferences, weight or BMI changes |
Dietary intake | Energy intake, dietary intake, food intake, carbohydrate intake, sugar intake, salt intake, water intake |
Glycemic | Glucose, Hemoglobin A1c (HbA1c), insulin concentration, insulin sensitivity, hypoglycemia, glucagon, glucose-dependent insulinotropic peptide (GIP), glucagon-like peptide-1 (GLP-1), peptide tyrosine tyrosine (PYY), cholecystokinin (CCK), enterostatin, ghrelin, leptin, somatostatin, oxyntomodulin |
Most recents protocols related to «Glucagon-Like Peptide 1»
Example 11
CREB responsive luciferase stable HEK 293 cell line overexpressing human glucagon receptor (GCGR), glucagon-like peptide 1 receptor (GLP-1R), Glucose-dependent insulinotropic polypeptide receptor (GIPR), or Glucagon-like peptide 2 receptor (GLP-2R) was generated as follows.
HEK293 cells were infected with lent virus encoding firefly luciferase gene under the control of CRE promoter, as described in the manual (Qiagen, Netherlands) and then were selected using 1p g/mL puromycin (Life technologies, Carlsbad) for 1 week. The survived cells were named as CRE-HEK293, expanded and then transfected with a G418 selective mammalian expression plasmid encoding human GCGR, GLP-1R, GIPR or GLP-2R. In brief, GCGR, GLP-1R, GIPR, or GLP-2R plasmid was transfected into CRE-HEK293 cells using Lipofectamine 2000 and selected with 400 μg/mL geneticin (Life technologies, Carlsbad, CA). Single colony stable cell lines overexpressing both CRE-luciferase and GCGR, GLP-1R, GIPR, or GLP-2R were then established for in vitro activity assays. These four stable cell lines were named as HEK293-GCGR-CRE, HEK293-GLP-1R-CRE, HEK293-GIPR-CRE, and HEK293-GLP-2R-CRE.
For ITTs, mice were fasted 3 h and injected with 1U/kg body weight insulin (Actrapid Penfill Insulin 100 IU/mL, Novo Nordisk). Glucose levels were measured at 0, 15, 30, 60, 90, and 120 min after injection.
For GLP-1 measurements, mice were i.p. injected with 25 mg/kg body weight Sitagliptin (Cat# sc-364620, Santa Cruz) 30 min prior to oral glucose administration and blood collected in diprotein A (Cat# I9759, Bachem). To block GLP-1 signaling, synthetic exendin (9–39) 25 nM/kg body weight (Cat# H-8740, Bachem) was injected i.p. 1 min prior to GTT.
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More about "Glucagon-Like Peptide 1"
This versatile peptide stimulates insulin secretion, suppresses glucagon release, and slows gastric emptying, leading to improved glycemic control.
Additionally, GLP-1 has been implicated in appetite regulation and weight management, making it a key target for the development of effective treatments for type 2 diabetes and other metabolic disorders.
The mechanisms and functions of GLP-1 are not limited to its role in glucose regulation.
GLP-1 receptor agonists and dipeptidyl peptidase-4 (DPP-4) inhibitors, also known as 'gliptins,' have emerged as potent therapeutic options for managing type 2 diabetes.
DPP-4 inhibitors work by preventing the breakdown of GLP-1, thereby enhancing its effects and improving glycemic control.
Understanding the intricate workings of GLP-1 is crucial for ongoing research and the development of novel strategies to address metabolic disorders.
Researchers can leverage cutting-edge tools like PubCompare.ai to optimize their GLP-1-related studies, accessing the best reproducible and accurate protocols from literature, preprints, and patents.
By harnessing the power of artificial intelligence, scientists can enhance their GLP-1 research and drive groundbreaking scientific discoveries.
Key subtopics and related terms in the GLP-1 research landscape include EZGLP1T-36K, EGLP-35K, Aprotinin, FBS (fetal bovine serum), and the GLP-1 active ELISA kit.
These tools and techniques are instrumental in advancing our understanding of this dynamic hormone and its potential therapeutic applications.
In summary, the exploration of Glucagon-Like Peptide 1 and its associated therapies, such as GLP-1 receptor agonists and DPP-4 inhibitors, holds immense promise for the management of metabolic disorders and the pursuit of improved health outcomes.
By combining the insights from the scientific literature with the power of AI-driven protocol optimization, researchers can unlock new frontiers in GLP-1 research and drive impactful discoveries.