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Growth Factor

Growth factors are signaling proteins that stimulate cell growth, proliferation, and differentiation.
They play crucial roles in tissue development, repair, and homeostasis.
Key growth factors include Fibroblast Growth Factors, Epidermal Growth Factor, Transforming Growth Factors, and Platelet-Derived Growth Factor.
These molecules bind to specific cell surface receptors, triggering intracellular signaling cascades that regulate gene expression and cellular processes.
Dysregulation of growth factor signaling is implicated in various pathological conditions, such as cancer, fibrosis, and impaired wound healing.
Understanidng growth factor biology is essential for advancing tissue engineering, regenerative medicine, and targeted therapies.

Most cited protocols related to «Growth Factor»

1
Directed Differentiation of Stem Cells to Intestinal Lineage
Cited 91 times
Human embryonic stem cells and induced pluripotent stem cells were maintained on Matrigel (BD Biosciences) in mTesR1 media without feeders. Differentiation into Definitive Endoderm was carried out as previously described 11 (link). Briefly, a 3-day ActivinA (R&D systems) differentiation protocol was used. Cells were treated with ActivinA (100ng/mL) for three consecutive days in RPMI 1640 media (Invitrogen) with increasing concentrations of 0%, 0.2%, 2% HyClone defined FBS (dFBS) (Thermo Scientific). For hindgut differentiation, DE cells were incubated in 2% dFBS-DMEM/F12 with 500ng/ml FGF4 and 500ng/ml Wnt3a (R&D Systems) for up to 4 days. Between 2 and 4 days with treatment of growth factors, 3-dimensional floating spheroids formed and were then transferred into three-dimensional cultures previously shown to promote intestinal growth and differentiation 15 (link),16 (link). Briefly, spheroids were embedded in Matrigel (BD Bioscience) containing 500ng/mL R-Spondin1 ( R&D Systems), 100ng/mL Noggin (R&D Systems) and 50ng/mL EGF (R&D Systems). After the Matrigel solidified, media (Advanced DMEM/F12 (Invitrogen) supplemented with L-Glutamine, 10 μM Hepes, N2 supplement (R&D Systems), B27 supplement (Invitrogen), and Pen/Strep containing growth factors was overlaid and replaced every 4 days.
Spence J.R., Mayhew C.N., Rankin S.A., Kuhar M., Vallance J.E., Tolle K., Hoskins E.E., Kalinichenko V.V., Wells S.I., Zorn A.M., Shroyer N.F, & Wells J.M. (2010). Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 470(7332), 105-109.
Publication 2010
Cells Dietary Supplements Endoderm FGF4 protein, human Glutamine Growth Factor HEPES Human Embryonic Stem Cells Induced Pluripotent Stem Cells Intestinal Neoplasms matrigel noggin protein Streptococcal Infections
2
Isolation of Normal and Neoplastic Pancreatic Ducts
Cited 72 times

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Publication 2014
Collagenase Dietary Fiber Digestion dispase Enzymes Growth Factor matrigel Neoplasm Metastasis Neoplasms Pancreas Pancreatic Duct RNA-Seq Tissues
3
Neural Induction of Pluripotent Stem Cells
Cited 65 times
For dissociating intact colonies of pluripotent stem cells from the layer of DR4 feeders, hiPSCs were exposed to a low concentration of dispase (Invitrogen: 17105-041; 0.7 mg/ml) for ~30 min. Suspended colonies were subsequently transferred into ultra-low-attachment 100 mm plastic plates (Corning) in hiPSC medium without FGF2. For the first 24 h (day 0), the medium was supplemented with the ROCK inhibitor Y-27632 (EMD Chemicals). For neural induction, dorsomorphin (also known as compound C; Sigma 10 μM) and SB-431542 (Tocris, 10 μM) were added to the medium for the first five days. On the sixth day in suspension, the floating spheroids were moved to neural medium (NM) containing Neurobasal (Invitrogen: 10888), B-27 serum substitute without vitamin A (Invitrogen: 12587), GlutaMax (Invitrogen, 1:100), 100 U/ml penicillin and 100 μl streptomycin (Invitrogen). The NM was supplemented with 20 ng/ml FGF2 (R&D Systems) and 20 ng/ml EGF (R&D Systems) for 19 days with daily medium change in the first 10 days, and every other day for the subsequent 9 days. To promote differentiation of the neural progenitors into neurons, FGF2 and EGF were replaced with 20 ng/ml BDNF (Peprotech) and 20 ng/ml NT3 (Peprotech) starting at day 25, while from day 43 onwards only NM without growth factors was used for medium changes every four days.
Paşca A.M., Sloan S.A., Clarke L.E., Tian Y., Makinson C.D., Huber N., Kim C.H., Park J.Y., O’Rourke N.A., Nguyen K.D., Smith S.J., Huguenard J.R., Geschwind D.H., Barres B.A, & Paşca S.P. (2015). Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nature methods, 12(7), 671-678.
Publication 2015
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide dispase dorsomorphin Feeder Cell Layers Fibroblast Growth Factor 2 Growth Factor Human Induced Pluripotent Stem Cells Nervousness Neurons Penicillins Pluripotent Stem Cells Serum Streptomycin Vitamin A Y 27632
4
Pluripotent Stem Cell Cardiac Differentiation
Cited 46 times
The following matrices were assessed for the ability to support pluripotent growth and subsequent cardiac differentiation: 9 µg/cm2 growth-factor reduced Matrigel (1:200, Corning) in DMEM/F12; 625 ng/cm2 vitronectin peptide (Synthemax II-SC, 1:320, Corning) in ultrapure water (1:50 also tested); 1 µg/cm2 full length recombinant human vitronectin (1:50, Primorigen) in D-PBS with CaCl2 and MgCl2; 2.5 µg/cm2 laminin-521 (1:80, Biolamina) in DPBS; 2 µg/cm2 truncated recombinant human laminin-511 iMatrix-511 (1:50, Iwai North America, Foster City, CA, USA) in DPBS; 1 µg/cm2 rH E-cadherin (1:25, StemAdhere, Primorigen/Stemcell Technologies); and 10 µg/cm2 fibronectin (1:20, EMD Millipore) in D-PBS. All were used at 2 mL per well of a 6-well (9.6 cm2). Matrices were assessed on both 6-well polystyrene tissue culture plates and untreated plates (both from Greiner). Also tested were Synthemax-T 6-well plates, and fibronectin mimetic plates (both from Corning) and 10 µg/cm2 Pronectin (Sigma-Aldrich).
Burridge P.W., Matsa E., Shukla P., Lin Z.C., Churko J.M., Ebert A.D., Lan F., Diecke S., Huber B., Mordwinkin N.M., Plews J.R., Abilez O.J., Cui B., Gold J.D, & Wu J.C. (2014). Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes. Nature methods, 11(8), 855-860.
Publication 2014
Cadherins FN1 protein, human Growth Factor Heart Homo sapiens Laminin laminin-511, human Magnesium Chloride matrigel Peptides Polystyrenes Stem Cells Tissues Vitronectin
5
Isolation and Culture of Glioblastoma Stem Cells
Cited 44 times

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Publication 2013
accutase Adenovirus Vaccine Cells Cloning Vectors Culture Media Cytokine Diagnosis Ethics Committees, Research Growth Factor Homo sapiens IL10 protein, human isolation Malignant Neoplasms Neoplasms Patients PDGFD protein, human Tissues Transforming Growth Factor beta Tumor Necrosis Factor-alpha

Most recents protocols related to «Growth Factor»

1
Haematopoietic Cell Expansion and Differentiation
Not available on PMC !

Example 5

Expansion and Differentiation of Haematopoietic Cells

The haematopoietic cells (e.g. haematopoietic stem cells) are stimulated using a supernatant growth factor suspension, to either develop more stem cells or differentiate into precursor cells (e.g. myeloid or granulocyte progenitor cells) or granulocytes. Suitable neutrophil synthesis methods are disclosed in Lieber et al, Blood, 2004 Feb. 1; 103(3):852-9, and Choi et al, Nat. Protoc., 2011 March; 6(3):296-313.

The protocol is composed of four major stages:

    • culturing and proliferation of haematopoietic cells;
    • short-term expansion of multipotent myeloid progenitors with a high dose of granulocyte-macrophage colony-stimulating factor (GM-CSF), a granulocyte colony-stimulating factor (G-CSF), a human growth hormone (HGH); serotonin, vitamin C, vitamin D, glutamine (Gln), arachidonic acid, AGE-albumin, interleukin-3 (IL-3), interleukin 8 (IL-8), Interleukin-4 (IL-4), Interleukin-6 (IL-6), interleukin-18 (IL-18), TNF-alpha, Flt-3 ligand, thrombopoietin, foetal bovine serum (FBS), or combinations thereof; and
    • directed differentiation of myeloid progenitors into neutrophils, eosinophils, dendritic cells (DCs), Langerhans cells (LCs), macrophages and osteoclasts.

US11878033B2. Cancer-killing cells (2024-01-23). LIFT BIOSCIENCES LTD [GB]. Inventors: Alex Blyth [GB], Nico Bruyniks [GB].
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Patent 2024
Albumins Anabolism Arachidonic Acid Ascorbic Acid BLOOD Cell Proliferation Cells Dendritic Cells Eosinophil Ergocalciferol Fetal Bovine Serum flt3 ligand Glutamine Granulocyte Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte Colony-Stimulating Factor Granulocyte Progenitor Cells Growth Factor Hematopoietic System Interleukin-3 Interleukin-18 interleukin 18 protein, human Langerhans Cell Macrophage Malignant Neoplasms Neutrophil Osteoclasts Serotonin Stem Cells Stem Cells, Hematopoietic Thrombopoietin Tumor Necrosis Factor-alpha
2
Establishment and Characterization of PDTO
Tumor pieces will be dissociated enzymatically and mechanically to obtain isolated cells or small cell clusters (Fig. 2). Cells will be embedded in an extracellular matrix (growth factor-reduced Matrigel or BME II) and cultured in a medium supplemented with growth factors and signal pathway inhibitors [Advanced DMEM (Gibco) supplemented with 100 UI/mL of penicillin and streptomycin (Gibco), 1% GlutaMAX (Gibco), 1X B27 (Gibco), 1.25 mM NAC (Sigma-Aldrich), 50 ng/mL EGF (PeproTech), 10 ng/mL FGF-10 (PeproTech), 5 ng/mL FGF-b (PeproTech), 500 nM A-83-01 (PeproTech), 10 μM Y27632 (Interchim), 10 mM Nicotinamide (Sigma-Aldrich), 1 μM PGE2 (PeproTech), 1 μM Forskolin (Peprotech), 0.3 μM CHIR99021 (Biogems), 100 μg/mL Primocin (InvivoGen), 50% Wnt3a, RSPO3, Noggin-conditioned media (L-WRN, ATCC), and 10% RSPO1-conditioned media (Cultrex HA-R-Spondin-1-Fc 293 T, Amsbio)]. Culture medium will be changed twice a week. Once formed, PDTO will be dissociated and reseeded to amplify them for experimental purposes. Cryovials will be prepared at regular intervals by dissociating and resuspending PDTO in Recovery Cell Culture Freezing Medium (Gibco) prior to be biobanked in liquid nitrogen. It should be noted that PDTO line will be considered as established when it will be maintained for more than 3 passages. For each PDTO line, samples will be kept frozen for DNA/RNA/protein analysis and others will be embedded in paraffin for histopathological analysis. This will allow comparisons between the characteristics of the PDTO and the tumor from which they are derived in order to validate their correspondence.

Establishment and characterization of PDTO derived from HNSCC and evaluation of response to treatments to assess its predictive value

Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
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Publication 2023
Cell Culture Techniques Cells Chir 99021 Colforsin Culture Media Culture Media, Conditioned Dinoprostone Extracellular Matrix Freezing Growth Factor HSP40 Heat-Shock Proteins inhibitors matrigel Neoplasms Niacinamide Nitrogen noggin protein Paraffin Embedding Penicillins Signal Pathways Squamous Cell Carcinoma of the Head and Neck Streptomycin Y 27632
3
Establishing 3D Cell Cultures for Longitudinal Imaging
A visual inspection was carried out after 24 h to identify plates with consistent confluence across wells. Cells were washed in PBS, trypsinized as described above and resuspended in PC3 medium. Typically, one-half to two-thirds of cells from each well were used for subsequent 3D cultures depending upon the initial confluence and effectiveness of trypsinization. Medium was supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences) and added to 96-well ImageLock plates (Satorius) pre-coated with 10 μl of GFRM for 15 min at 37°C. For live imaging, ImageLock plates were incubated at 37°C for 4 h, then imaged using an IncuCyte ZOOM (Satorius) with IncuCyte ZOOM Live Cell Analysis System Software 2018A. Phase and GFP images were taken every hour for 4 d at two positions per well with ×10 objective lens.
Sandilands E., Freckmann E.C., Cumming E.M., Román-Fernández A., McGarry L., Anand J., Galbraith L., Mason S., Patel R., Nixon C., Cartwright J., Leung H.Y., Blyth K, & Bryant D.M. (2023). The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels. The Journal of Cell Biology, 222(4), e202206115.
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Publication 2023
Cells Growth Factor Lens, Crystalline matrigel
4
Neuroinflammatory Profile in Cocaine Abstinence

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Publication 2023
Animals Biological Assay Brain Buffers Caspase 1 Cell Respiration Chemokine Cocaine Cold Temperature Cytokine Decapitation Growth Factor inhibitors Isoflurane Phosphoric Monoester Hydrolases Radioimmunoprecipitation Assay succinyl-trialanine-4-nitroanilide Tissues
5
Gp120 and MC-25-41 Effects on Cocaine Craving

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Publication 2023
Animals Chemokine Cocaine Cyclodextrins Cytokine Growth Factor HIV Envelope Protein gp120 Microinjections NOAC protocol Saline Solution Self Administration Ventricle, Lateral

Top products related to «Growth Factor»

1
Matrigel by BD
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
2
Growth factor reduced matrigel by BD
Sourced in United States, United Kingdom, Germany, Canada, Belgium, Australia, China, Morocco, Italy
Growth factor-reduced Matrigel is a complex extracellular matrix (ECM) preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is a soluble basement membrane extract that solidifies to form a gel-like material when warmed to physiological temperature.
3
Matrigel by Corning
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
4
Growth factor reduced matrigel by Corning
Sourced in United States, Canada, Japan, China, France, Germany, United Kingdom
Growth factor reduced Matrigel is a soluble basement membrane extract derived from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is a complex mixture of extracellular matrix proteins, including laminin, collagen IV, heparan sulfate proteoglycans, and enactin. This product has been processed to reduce the levels of growth factors compared to regular Matrigel.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
7
Epidermal growth factor (egf) by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, Canada, France, Italy, Japan, Israel, Switzerland, Australia, Macao, Belgium, Spain, Denmark, Jersey
EGF is a lab equipment product from Thermo Fisher Scientific. It is a recombinant human Epidermal Growth Factor (EGF) protein. EGF is a growth factor that plays a role in cell proliferation and differentiation.
8
Glutamax by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Switzerland, Canada, Japan, Australia, China, Belgium, Italy, Denmark, Spain, Austria, Netherlands, Sweden, Ireland, New Zealand, Israel, Gabon, India, Poland, Argentina, Macao, Finland, Hungary, Brazil, Slovenia, Sao Tome and Principe, Singapore, Holy See (Vatican City State)
GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
9
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
10
Dmem f12 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Japan, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Denmark, Israel, Macao, Ireland, Netherlands, Austria, Hungary, Holy See (Vatican City State), Sweden, Brazil, Argentina, India, Poland, Morocco, Czechia
DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.

More about "Growth Factor"

Growth factors are a class of signaling molecules that play a crucial role in regulating cellular processes, such as growth, proliferation, and differentiation.
These biomolecules, which include Fibroblast Growth Factors (FGFs), Epidermal Growth Factor (EGF), Transforming Growth Factors (TGFs), and Platelet-Derived Growth Factor (PDGF), bind to specific cell surface receptors and trigger intracellular signaling cascades.
This activation of signaling pathways ultimately leads to the modulation of gene expression and cellular activities, which are essential for tissue development, repair, and homeostasis.
Matrigel and growth factor-reduced Matrigel are commonly used in cell culture and tissue engineering applications as they provide a biologically relevant extracellular matrix (ECM) that supports cell attachment, proliferation, and differentiation.
These specialized matrices often contain a blend of growth factors, such as EGF and FGF, which can further enhance cellular responses.
Supplementing cell culture media with fetal bovine serum (FBS) and GlutaMAX can also provide additional growth factors and nutrients to support cell growth and proliferation.
Dysregulation of growth factor signaling is implicated in various pathological conditions, including cancer, fibrosis, and impaired wound healing.
Understanding the complex biology of growth factors is crucial for advancing tissue engineering, regenerative medicine, and the development of targeted therapies.
PubCompare.ai, an AI-driven platform, can enhance growth factor research by providing optimized protocol discovery and improving reproducibility through advanced comparisons of protocols from literature, preprints, and patents.