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H 450

H 450 is a unique, yet understudied, protein that plays a critical role in cellular processes.
This protein is involved in a variety of physiological functions, including cell signaling, metabolism, and immune response.
Researchers are actively investigating the potential therapeutic applications of H 450, as it may be a promising target for the treatment of various diseases.
However, the reproducibility of research on H 450 has been a challenge, due to the complexity of experimental protocols and the lack of standardized methods.
PubCompare.ai aims to revolutionize this field by providing AI-powered protocol optimization and seamless access to the latest research on H 450, including literature, preprints, and patents.
By leveraging PubCompare.ai's tools, researchers can easily identify the best protocols and products for their H 450 research, leading to improved reproducibility and accelerated scientific discovery.

Most cited protocols related to «H 450»

Different sample types were subjected to bead-based multiplex EV analysis by flow cytometry (MACSPlex Exosome Kit, human, Miltenyi Biotec) (9 (link), 10 (link)), with details regarding sample preparation and normalization summarized in Table S2 in Supplementary Material. Unless indicated otherwise, EV-containing samples were processed as follows: Samples were diluted with MACSPlex buffer (MPB) to, or used undiluted at, a final volume of 120 µL and loaded onto wells of a pre-wet and drained MACSPlex 96-well 0.22 µm filter plate before 15 µL of MACSPlex Exosome Capture Beads (containing 39 different antibody-coated bead subsets) were added to each well. Generally, particle counts quantified by NTA, and not protein amount, were used to estimate input EV amounts. Filter plates were then incubated on an orbital shaker overnight (14–16 h) at 450 rpm at room temperature protected from light. To wash the beads, 200 µL of MPB was added to each well and the filter plate was put on a vacuum manifold with vacuum applied (Sigma-Aldrich, Supelco PlatePrep; −100 mBar) until all wells were drained. For counterstaining of EVs bound by capture beads with detection antibodies, 135 µL of MPB and 5 µL of each APC-conjugated anti-CD9, anti-CD63, and anti-CD81 detection antibody were added to each well and plates were incubated on an orbital shaker at 450 rpm protected from light for 1 h at room temperature. Next, plates were washed by adding 200 µL MPB to each well followed by draining on a vacuum manifold. This was followed by another washing step with 200 µL of MPB, incubation on an orbital shaker at 450 rpm protected from light for 15 min at room temperature and draining all wells again on a vacuum manifold. Subsequently, 150 µL of MPB was added to each well, beads were resuspended by pipetting and transferred to V-bottom 96-well microtiter plate (Thermo Scientific). Flow cytometric analysis was performed, unless indicated otherwise, with a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec; see Table S1 in Supplementary Material for acquisition parameters) by using the built-in 96-well plate reader. All samples were automatically mixed immediately before 70–100 µL were loaded to and acquired by the instrument, resulting in approximately 7,000–12,000 single bead events being recorded per well. FlowJo software (v10, FlowJo LLC) was used to analyze flow cytometric data. Median fluorescence intensity (MFI) for all 39 capture bead subsets were background corrected by subtracting respective MFI values from matched non-EV buffer or media controls that were treated exactly like EV-containing samples (buffer/medium + capture beads + antibodies). GraphPadPrism 6 (GraphPadPrism Software, La Jolla, CA, USA) was used to analyze data and assemble figures. To generate heatmaps of data, flow cytometric data were gated in FlowJo with gated data being exported to comma separated files, which were subsequently imported into MATLAB (v9.3.0, Mathworks Inc.) for further analysis and data visualization. In order to compare data from the MACSQuant and FACS Symphony flow cytometers, the log10 transformed ratios of capture beads + EVs + Ab over their respective controls (capture beads + ab) was compared, rather than using background subtraction, which allowed for comparison despite axis scaling differences.
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Publication 2018
Antibodies Antibodies, Anti-Idiotypic Buffers Epistropheus Exosomes Flow Cytometry Fluorescence H 450 Homo sapiens Immunoglobulins Light Proteins Strains Vacuum
Fresh leaves were plucked from a single cultivated alfalfa (cultivar XinJiangDaYe) plant cultivated in a greenhouse kept at 21–23 °C, 16 h light per day (light intensity of 380–450 W per m2) and a relative humidity (RH) of 70%. DNA was extracted from these leaves using a DNeasy Plant Mini Kit (Qiagen). Portions of the DNA were sent to AnnoRoad (Ningbo, China) to construct circular consensus sequencing (CCS) libraries and sequence them using a PacBio Sequal platform, and other portions were sent to Nextomics (Wuhan, China) to construct libraries and sequence them using Nanopore ONT and Illumina Hiseq platforms. These sequencing efforts yielded 70, 99, and 126 Gb of reads, respectively, for de novo assembly of the cultivated alfalfa genome (Supplementary Tables 1 and 2).
In addition, tender roots and shoots with leaves were collected, and RNA was extracted from one pooled root and shoot sample (with roughly the same weight of each organ) and four leaf samples using an RNeasy Plant Mini Kit (Qiagen). RNA samples were reverse-transcribed using random primers and sequenced using an Illumina platform. The RNA-seq data obtained are summarized in Supplementary Table 9.
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Publication 2020
Alfalfa Genome H 450 Humidity Light Oligonucleotide Primers Plant Leaves Plant Roots Plants RNA-Seq

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Publication 2014
Agaricales Biological Assay Buffers Cells Centrifugation dopachrome Enzymes H 450 kojic acid Levodopa Melanoma, B16 Monophenol Monooxygenase Phosphates Proteins Serum Albumin, Bovine Triton X-100 Vision
The Ge precursors were deposited on SiO2 glass substrates using the Knudsen cell of a molecular beam deposition system (base pressure: 5 × 10−7 Pa). The deposition rate was 1.0 nm/min where the sample substrate was not heated. The deposition time was 100 min. The Ge source, manufactured by Furuuchi Chemical Corporation, had a purity of 99.999%. The substrate temperature during the deposition, Td, ranged from 50 to 200 °C. We note that Td spontaneously rises from room temperature to 50 °C without heating the substrate because of the heat propagation from the Knudsen cell. The samples were then loaded into a conventional tube furnace in a N2 atmosphere and annealed at 375 °C for 140 h, 400 °C for 40 h, and 450 °C for 5 h to induce SPC.
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Publication 2017
Atmosphere Cells H 450 Pressure
The ceramic slurry for scaffold fabrication was prepared by gradually adding 60 or 65 g of TiO2 powder to 25 ml of sterilised water and the pH of the slurry was kept at 1.7 for the entire duration of the stirring with small additions of 1 M HCl. For double-coated scaffolds, the slurry for the second coating contained 40 g of TiO2 powder in 25 ml sterilised water. The TiO2 powder was stirred in to the water in several steps at low rotation speed of 1,000 rpm (Dispermat Ca-40, VMA-Getzmann GmbH, Reichshof, Germany). The temperature of the slurry was adjusted to room temperature during the low rotation speed stirring. When the slurry mixture was homogenous, the rotation speed was increased to 5,000 rpm and the slurry was stirred for 5 h at this rotation speed. Temperature of the slurry was reduced to 15°C during high speed stirring.
The polyurethane (PU) foam templates (60 ppi, Bulbren S, Eurofoam GmbH, Wiesbaden, Germany) were washed and cut to appropriate size as described in [18 (link)]. The cylindrical templates were then immersed in the ceramic slurry, and excess slurry was either squeezed between two polymer foam sheets or centrifuged (1 min @ 1,000 rpm; Biofuge 22R Heraeus Sepatech, Osterode, Germany) out of the foam templates to ensure that only a thin layer of slurry covered uniformly the entire surface area of the polymer template without blocking the pores. The samples were then placed on a porous ceramic plate and allowed to dry at room temperature for at least 16 h before sintering.
For the burnout of the polymer, the scaffolds were slowly heated to 450°C at a heating rate of 0.5 K/min. After 1 h holding time at 450°C, temperature was raised to 1,500°C at a rate of 3 K/min and the sintering time at this temperature was set to 40 h. The sintered scaffolds were then cooled back to room temperature at the cooling rate of 5 K/min (HTC-08/16, Nabertherm GmbH, Bremen, Germany).
Publication 2010
Burnout, Psychological Cardiac Arrest H 450 Homozygote Polymers polyurethane foam Powder

Most recents protocols related to «H 450»

Example 48

[Figure (not displayed)]

A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1R)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-b]pyridazine (500 mg, 1.21 mmol) and AgOTf (3120.6 mg, 12.15 mmol) in 2-methylpropan-1-ol (10 mL, 1.21 mmol) and CH3CN (10 mL) was stirred at 90° C. for 8 days. After cooling to room temperature, the reaction was diluted with EtOAc (40 mL), and the mixture was added with brine (40 mL), the mixture was filtered through Celite, and eluted with EtOAc (20 mL×2), and the filtrate was concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=0% to 50% to 100%) to give the impure product. The impure product was purified by Prep-HPLC (Waters Xbridge 150 mm×25 mm, 5 μm) A=H2O (10 mM NH4HCO3) and B=CH3CN; 54-84% B over 8 minutes) to give the product (53.85 mg, 0.12 mmol, 10% yield) as a solid. 1H NMR (CDCl3, 400 MHz) δH=8.54 (d, 1H), 8.30 (d, 1H), 8.13 (dd, 1H), 7.66 (d, 1H), 5.92 (spt, 1H), 4.01 (d, 2H), 2.16-2.04 (m, 1H), 1.61 (d, 3H), 1.05 (d, 6H). LCMS Rt=1.42 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. for C18H18F6N5O2 [M+H]+ 450.1. found 450.1.

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Patent 2024
1H NMR brine Celite Chromatography H 450 High-Performance Liquid Chromatographies Lincomycin pyridazine Silica Gel

Example 44

[Figure (not displayed)]

A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1S)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-a]pyrazine (200 mg, 0.49 mmol) and AgOTf (1.25 g, 4.86 mmol) in Isobutyl alcohol (10 mL) and MeCN (10 mL) was stirred at 90° C. for 7 days. The EtOAc (50 mL) and brine (50 mL) were added to the mixture and some solid was observed. The mixture was filtered through Celite. The filtrate was separated and the aqueous layer was extracted with EtOAc (50 mL). The combined organic phase was dried over anhydrous Na2SO4, filtered and concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=10% to 30% to 50%) to give the product (80 mg) as an oil.

The impure product (80 mg, 0.18 mmol) was purified by Prep-HPLC (Boston Green ODS (150 mm×30 mm, 5 μm) A=H2O (0.075% TFA) and B=CH3CN; 66-96% B over 8 minutes) and concentrated to give a residue. To the residue was added saturated aqueous NaHCO3 (10 mL), and the mixture was extracted with EtOAc (15 mL×2). The combined organic phase was washed with brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated to give the product (55.97 mg, 124.6 μmol, 70% yield) as a solid. 1H NMR (CDCl3+D2O, 400 MHz) δH=9.52 (d, 1H), 8.53-8.43 (m, 2H), 8.04 (dd, 1H), 5.98-5.84 (m, 1H), 4.06 (d, 2H), 2.21-2.06 (m, 1H), 1.60 (d, 3H), 1.08 (d, 6H). LCMS Rt=1.37 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. for C18H18F6N5O2 [M+H]+450.1. found 450.0.

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Patent 2024
1H NMR Bicarbonate, Sodium brine Celite Chromatography H 450 High-Performance Liquid Chromatographies isobutyl alcohol Lincomycin Pyrazines Silica Gel

Example 46

[Figure (not displayed)]

A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1S)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-b]pyridazine (300 mg, 0.73 mmol) and AgOTf (1872.33 mg, 7.29 mmol) in 2-methylpropan-1-ol (10 mL, 0.73 mmol) and MeCN (10 mL) was stirred at 90° C. for 9 days. After cooling to room temperature, the reaction was diluted with EtOAc (60 mL) and brine (20 mL), filtered through Celite, and extracted with EtOAc (50 mL×2). The combined organic layer was washed with water (50 mL) and brine (50 mL), dried over Na2SO4, filtered and concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=0% to 70%) The isolated product was further purified by Prep-HPLC (Boston Prime C18 (150 mm×30 mm, 5 μm), A=H2O (0.05% NH4OH) and B=CH3CN; 60-90% B over 9 minutes) to give the product (7.42 mg, 16.5 μmol, 2% yield) as a solid. 1H NMR (CDCl3, 400 MHz) δH=8.54 (d, 1H), 8.30 (d, 1H), 8.14 (dd, 1H), 7.66 (d, 1H), 5.98-5.85 (m, 1H), 4.01 (d, 2H), 2.15-2.01 (m, 1H), 1.61 (d, 3H), 1.05 (d, 6H). LCMS Rt=1.39 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. C18H18F6N5O2 [M+H]+ 450.1. found 450.1.

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Patent 2024
1H NMR brine Celite Chromatography H 450 High-Performance Liquid Chromatographies Lincomycin pyridazine Silica Gel
The synthesis of titanium dioxide (TiO2) NPs was carried using the sol–gel method. TiO2 NPs were prepared according to the procedure described by Noorimotlagh et al. [39 (link)]. Briefly, 2-propanol solution was added drop wise in TTIP solution under continuous stirring (solution A). Then, for TiO2 doping with ZnO and Fe2O3, a specific amount of ZnO and Fe2O3 NPs with 0.02% ratio was added in 40 mL deionized water (solution B). Afterward, addition of solution B to A was carried out in drop wise under continuous stirring to produce the TiO2–FeZn NPs in gel form. Then, the gel was centrifuged, rinsed with deionized water, kept in an oven, ground and annealed in a muffle furnace (FP125 model) at 450 °C during 4 h. The experimental equipment applied to characterization of TiO2–FeZn nanocatalyst properties is shown in Table 1.

Experimental equipment applied to characterize properties of TiO2–FeZn NPs nanocatalyst

MethodEquipment modelApplication
SEMaTescan, Mira3, Czech RepublicDetermination of morphological properties of synthesized NPs
XRDbPhilips Xpert diffractometerDetermination of the crystalline structure of the synthesized NPs
EDXcPHILIPS, XL-30, NetherlandsDetermination of the elemental composition of synthesized NPs
FT-IRdSt-Jeam Baptiste Bomem 450Determination of the functional groups of synthesized NPs
BETeBET- Micromeritics Tristar 3000Determination of the specific surface area of synthesized NPs

aField emission scanning electron microscope

bX–ray diffraction pattern

cEnergy–dispersive X-ray spectroscopy

dFourier transform infrared spectrometer

eBrunauer–Emmett–Teller method

Publication 2023
Anabolism Electrons H 450 Isopropyl Alcohol Roentgen Rays titanium dioxide
UHPLC-Q-TOF/MS analysis was performed on an ACQUITY UHPLC™ system (Waters Corp. Milford, MA, United States) coupled with a Synapt G1 MS system (Waters Corp. Manchester, United Kingdom). A Waters ACQUITY UHPLC HSS T3 column (100 × 2.1 mm, 1.8 μm) was used for the analysis with the column temperature at 40°C. The mobile phases were water with 0.1% formic acid (A) and acetonitrile (B). The gradient used was as follows: 0–2 min, 5%→15% B; 2–18 min, 15%→37% B; 18–25 min, 37%→50% B; 25–27 min, 50% B; 27–28 min, 50%→5% B and 28–30 min, 5% B. The flow rate was 0.5 mL/min. The injection volume of the sample was 5 μL.
The data acquisition mode was MSE. The data were obtained at 50–1500 Da. The source temperature was 100°C, the desolvation temperature was 450 °C with desolvation gas flow 850 L/h, leucine enkephaline was used as lock mass, and the capillary voltage was 3 kV. At low CE scan, the cone voltage was 30 V for ESI, and the collision energy was 6 eV (trap) and 4 eV (transfer), while it was 40–60 eV ramp (trap) and 12 eV (transfer) for ESI and 15–25 eV ramp (trap) and 12 eV (transfer) for ESI+. The instrument was controlled by MassLynx 4.1 software (Waters Corp.).
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Publication 2023
acetonitrile Capillaries formic acid H 450 Leucine Radionuclide Imaging Retinal Cone

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The Cell Counting Kit-8 is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt that produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. The amount of the formazan dye generated is directly proportional to the number of living cells.
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CCK-8 is a cell counting kit used to measure cell viability and proliferation. It utilizes a water-soluble tetrazolium salt that is reduced by living cells, producing a colored formazan dye that can be quantified using a spectrophotometer. The amount of formazan dye produced is directly proportional to the number of living cells in the sample.
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Prep-HPLC is a high-performance liquid chromatography (HPLC) system designed for preparative-scale purification. It provides efficient separation and purification of compounds from complex mixtures.
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GF/F filters are a type of laboratory filtration equipment designed to remove very small particles from liquid samples. They are composed of glass fiber and have a nominal pore size of 0.7 micrometers, making them suitable for filtering a wide range of materials.
The Boston Prime C18 is a laboratory equipment product designed for chromatographic separations. It is a high-performance reversed-phase liquid chromatography (RPLC) stationary phase used for the analysis and purification of a wide range of organic compounds.
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GF/F glass fiber filters are a type of laboratory filter used for the retention of particles and microorganisms during filtration processes. These filters are made of glass fibers and are designed to provide efficient and consistent filtration performance.
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The Microplate reader is a laboratory instrument used to measure the absorbance or fluorescence of samples in a microplate format. It can be used to conduct various assays, such as enzyme-linked immunosorbent assays (ELISA), cell-based assays, and other biochemical analyses. The core function of the Microplate reader is to precisely quantify the optical properties of the samples in a multi-well microplate.
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The H-450 is a high-performance analytical instrument designed for materials science research. It utilizes advanced electron microscopy technology to provide detailed imaging and analysis of samples at the nanoscale level. The core function of the H-450 is to enable researchers to visualize and characterize the microstructure and composition of a wide range of materials with high resolution and accuracy.
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The CCK-8 (Cell Counting Kit-8) is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes the water-soluble tetrazolium salt WST-8 to produce a colored formazan dye upon reduction by dehydrogenases in living cells. The amount of the formazan dye generated is directly proportional to the number of viable cells.
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Prism 9 is a powerful data analysis and graphing software developed by GraphPad. It provides a suite of tools for organizing, analyzing, and visualizing scientific data. Prism 9 offers a range of analysis methods, including curve fitting, statistical tests, and data transformation, to help researchers and scientists interpret their data effectively.

More about "H 450"

H-450 is a vital, yet underexplored, biomolecule that plays a pivotal role in cellular processes.
This unique protein is involved in a diverse array of physiological functions, including cell signaling, metabolism, and immune response.
Researchers are actively investigating the potential therapeutic applications of H-450, as it may hold promise as a target for treating various diseases.
However, the reproducibility of H-450 research has been a challenge, due to the complexity of experimental protocols and the lack of standardized methods.
PubCompare.ai aims to revolutionize this field by providing AI-powered protocol optimization and seamless access to the latest research on H-450, including literature, preprints, and patents.
By leveraging PubCompare.ai's tools, researchers can easily identify the best protocols and products for their H-450 research, leading to improved reproducibility and accelerated scientific discovery.
This includes access to a wide range of related techniques and materials, such as Cell Counting Kit-8 (CCK-8), Prep-HPLC, GF/F filters, Boston Prime C18, GF/F glass fiber filters, and Microplate readers.
PubCompare.ai's AI-driven comparisons help researchers find the optimal protocols and products for their H-450 studies, enhancing the reliability and efficiency of their work.
With Prism 9, researchers can visualize and analyze their H-450 data more effectively, further improving the quality and reproducibility of their research.
By embracing the tools and resources offered by PubCompare.ai, researchers can overcome the challenges of H-450 research and make significant advancements in this critical area of biology.
Discover how PubCompare.ai can revolutionize your H-450 research and propel scientific discovery forward.