H 450
This protein is involved in a variety of physiological functions, including cell signaling, metabolism, and immune response.
Researchers are actively investigating the potential therapeutic applications of H 450, as it may be a promising target for the treatment of various diseases.
However, the reproducibility of research on H 450 has been a challenge, due to the complexity of experimental protocols and the lack of standardized methods.
PubCompare.ai aims to revolutionize this field by providing AI-powered protocol optimization and seamless access to the latest research on H 450, including literature, preprints, and patents.
By leveraging PubCompare.ai's tools, researchers can easily identify the best protocols and products for their H 450 research, leading to improved reproducibility and accelerated scientific discovery.
Most cited protocols related to «H 450»
In addition, tender roots and shoots with leaves were collected, and RNA was extracted from one pooled root and shoot sample (with roughly the same weight of each organ) and four leaf samples using an RNeasy Plant Mini Kit (Qiagen). RNA samples were reverse-transcribed using random primers and sequenced using an Illumina platform. The RNA-seq data obtained are summarized in Supplementary Table
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
The polyurethane (PU) foam templates (60 ppi, Bulbren S, Eurofoam GmbH, Wiesbaden, Germany) were washed and cut to appropriate size as described in [18 (link)]. The cylindrical templates were then immersed in the ceramic slurry, and excess slurry was either squeezed between two polymer foam sheets or centrifuged (1 min @ 1,000 rpm; Biofuge 22R Heraeus Sepatech, Osterode, Germany) out of the foam templates to ensure that only a thin layer of slurry covered uniformly the entire surface area of the polymer template without blocking the pores. The samples were then placed on a porous ceramic plate and allowed to dry at room temperature for at least 16 h before sintering.
For the burnout of the polymer, the scaffolds were slowly heated to 450°C at a heating rate of 0.5 K/min. After 1 h holding time at 450°C, temperature was raised to 1,500°C at a rate of 3 K/min and the sintering time at this temperature was set to 40 h. The sintered scaffolds were then cooled back to room temperature at the cooling rate of 5 K/min (HTC-08/16, Nabertherm GmbH, Bremen, Germany).
Most recents protocols related to «H 450»
Example 48
A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1R)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-b]pyridazine (500 mg, 1.21 mmol) and AgOTf (3120.6 mg, 12.15 mmol) in 2-methylpropan-1-ol (10 mL, 1.21 mmol) and CH3CN (10 mL) was stirred at 90° C. for 8 days. After cooling to room temperature, the reaction was diluted with EtOAc (40 mL), and the mixture was added with brine (40 mL), the mixture was filtered through Celite, and eluted with EtOAc (20 mL×2), and the filtrate was concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=0% to 50% to 100%) to give the impure product. The impure product was purified by Prep-HPLC (Waters Xbridge 150 mm×25 mm, 5 μm) A=H2O (10 mM NH4HCO3) and B=CH3CN; 54-84% B over 8 minutes) to give the product (53.85 mg, 0.12 mmol, 10% yield) as a solid. 1H NMR (CDCl3, 400 MHz) δH=8.54 (d, 1H), 8.30 (d, 1H), 8.13 (dd, 1H), 7.66 (d, 1H), 5.92 (spt, 1H), 4.01 (d, 2H), 2.16-2.04 (m, 1H), 1.61 (d, 3H), 1.05 (d, 6H). LCMS Rt=1.42 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. for C18H18F6N5O2 [M+H]+ 450.1. found 450.1.
Example 44
A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1S)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-a]pyrazine (200 mg, 0.49 mmol) and AgOTf (1.25 g, 4.86 mmol) in Isobutyl alcohol (10 mL) and MeCN (10 mL) was stirred at 90° C. for 7 days. The EtOAc (50 mL) and brine (50 mL) were added to the mixture and some solid was observed. The mixture was filtered through Celite. The filtrate was separated and the aqueous layer was extracted with EtOAc (50 mL). The combined organic phase was dried over anhydrous Na2SO4, filtered and concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=10% to 30% to 50%) to give the product (80 mg) as an oil.
The impure product (80 mg, 0.18 mmol) was purified by Prep-HPLC (Boston Green ODS (150 mm×30 mm, 5 μm) A=H2O (0.075% TFA) and B=CH3CN; 66-96% B over 8 minutes) and concentrated to give a residue. To the residue was added saturated aqueous NaHCO3 (10 mL), and the mixture was extracted with EtOAc (15 mL×2). The combined organic phase was washed with brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated to give the product (55.97 mg, 124.6 μmol, 70% yield) as a solid. 1H NMR (CDCl3+D2O, 400 MHz) δH=9.52 (d, 1H), 8.53-8.43 (m, 2H), 8.04 (dd, 1H), 5.98-5.84 (m, 1H), 4.06 (d, 2H), 2.21-2.06 (m, 1H), 1.60 (d, 3H), 1.08 (d, 6H). LCMS Rt=1.37 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. for C18H18F6N5O2 [M+H]+450.1. found 450.0.
Example 46
A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[(1S)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-b]pyridazine (300 mg, 0.73 mmol) and AgOTf (1872.33 mg, 7.29 mmol) in 2-methylpropan-1-ol (10 mL, 0.73 mmol) and MeCN (10 mL) was stirred at 90° C. for 9 days. After cooling to room temperature, the reaction was diluted with EtOAc (60 mL) and brine (20 mL), filtered through Celite, and extracted with EtOAc (50 mL×2). The combined organic layer was washed with water (50 mL) and brine (50 mL), dried over Na2SO4, filtered and concentrated to give the crude product. The crude product was purified by flash chromatography on silica gel (EtOAc in PE=0% to 70%) The isolated product was further purified by Prep-HPLC (Boston Prime C18 (150 mm×30 mm, 5 μm), A=H2O (0.05% NH4OH) and B=CH3CN; 60-90% B over 9 minutes) to give the product (7.42 mg, 16.5 μmol, 2% yield) as a solid. 1H NMR (CDCl3, 400 MHz) δH=8.54 (d, 1H), 8.30 (d, 1H), 8.14 (dd, 1H), 7.66 (d, 1H), 5.98-5.85 (m, 1H), 4.01 (d, 2H), 2.15-2.01 (m, 1H), 1.61 (d, 3H), 1.05 (d, 6H). LCMS Rt=1.39 min in 2.0 min chromatography, 10-80AB, MS ESI calcd. C18H18F6N5O2 [M+H]+ 450.1. found 450.1.
Experimental equipment applied to characterize properties of TiO2–FeZn NPs nanocatalyst
Method | Equipment model | Application |
---|---|---|
SEMa | Tescan, Mira3, Czech Republic | Determination of morphological properties of synthesized NPs |
XRDb | Philips Xpert diffractometer | Determination of the crystalline structure of the synthesized NPs |
EDXc | PHILIPS, XL-30, Netherlands | Determination of the elemental composition of synthesized NPs |
FT-IRd | St-Jeam Baptiste Bomem 450 | Determination of the functional groups of synthesized NPs |
BETe | BET- Micromeritics Tristar 3000 | Determination of the specific surface area of synthesized NPs |
aField emission scanning electron microscope
bX–ray diffraction pattern
cEnergy–dispersive X-ray spectroscopy
dFourier transform infrared spectrometer
eBrunauer–Emmett–Teller method
The data acquisition mode was MSE. The data were obtained at 50–1500 Da. The source temperature was 100°C, the desolvation temperature was 450 °C with desolvation gas flow 850 L/h, leucine enkephaline was used as lock mass, and the capillary voltage was 3 kV. At low CE scan, the cone voltage was 30 V for ESI, and the collision energy was 6 eV (trap) and 4 eV (transfer), while it was 40–60 eV ramp (trap) and 12 eV (transfer) for ESI− and 15–25 eV ramp (trap) and 12 eV (transfer) for ESI+. The instrument was controlled by MassLynx 4.1 software (Waters Corp.).
Top products related to «H 450»
More about "H 450"
This unique protein is involved in a diverse array of physiological functions, including cell signaling, metabolism, and immune response.
Researchers are actively investigating the potential therapeutic applications of H-450, as it may hold promise as a target for treating various diseases.
However, the reproducibility of H-450 research has been a challenge, due to the complexity of experimental protocols and the lack of standardized methods.
PubCompare.ai aims to revolutionize this field by providing AI-powered protocol optimization and seamless access to the latest research on H-450, including literature, preprints, and patents.
By leveraging PubCompare.ai's tools, researchers can easily identify the best protocols and products for their H-450 research, leading to improved reproducibility and accelerated scientific discovery.
This includes access to a wide range of related techniques and materials, such as Cell Counting Kit-8 (CCK-8), Prep-HPLC, GF/F filters, Boston Prime C18, GF/F glass fiber filters, and Microplate readers.
PubCompare.ai's AI-driven comparisons help researchers find the optimal protocols and products for their H-450 studies, enhancing the reliability and efficiency of their work.
With Prism 9, researchers can visualize and analyze their H-450 data more effectively, further improving the quality and reproducibility of their research.
By embracing the tools and resources offered by PubCompare.ai, researchers can overcome the challenges of H-450 research and make significant advancements in this critical area of biology.
Discover how PubCompare.ai can revolutionize your H-450 research and propel scientific discovery forward.