> Chemicals & Drugs > Amino Acid > Hemosiderin

Hemosiderin

Hemosiderin is an iron-containing pigment that is formed from the breakdown of hemoglobin.
It is found in various tissues, especially in the liver, spleen, and bone marrow, and is a marker of iron overload conditions.
Hemosiderin deposition can be associated with a variety of clinical conditions, including hemochromatosis, chronic liver disease, and certain hematological disorders.
The accurate identification and quantification of hemosiderin is crucial for the diagnosis and management of these conditions.
PubCompare.ai's AI-driven platform can help researchers optimize their hemosiderin research by easily locating the best protocols from literature, preprints, and patents, while comparing them to enhance reproducibility and acuracy.
Experience seamless research with their intuitive tools.

Most cited protocols related to «Hemosiderin»

Histologic Assessment of Fatty Liver Disease

Cited 6 times

Histologic features of fatty liver disease and iron staining pattern were assessed by the Pathology Committee of the NASH CRN in a centralized consensus review format using criteria previously described (20 (link)). Pathologists were blinded to all clinical, laboratory and demographic information. Iron stains were performed by a central lab using Perls’ iron stain; iron stains were scored prospectively by a method agreed upon by the Pathology Committee. Only granular iron deposition was scored, based on agreement that only discernible hemosiderin granules represent significant iron deposition (3 (link), 4 (link)). Hepatocellular iron was scored from 0 to 4 using the method of Rowe et al., with the modification that a 20x objective was used in place of the 25x objective (21 (link)). Non-hepatocellular iron (RES) was scored on a three point scale as none, mild or more than mild.

Nelson J.E., Wilson L., Brunt E.M., Yeh M.M., Kleiner D.E., Unalp-Arida A, & Kowdley K.V. (2010). RELATIONSHIP BETWEEN PATTERN OF HEPATIC IRON DEPOSITION AND HISTOLOGIC SEVERITY IN NONALCOHOLIC FATTY LIVER DISEASE. Hepatology (Baltimore, Md.), 53(2), 448-457.

Publication 2010

Clinical Laboratory Services Cytoplasmic Granules Hemosiderin Iron Liver Diseases Nonalcoholic Steatohepatitis Pathologists Staining Stains

Microscopic Analysis of Cerebrovascular Pathology

Cited 4 times

Digital brightfield microscopic images of the sections were obtained with the Hamamatsu NanoZoomer Digital Pathology (NDP)-HT whole slide scanner (C9600-12, Hamamatsu Photonics KK, Japan) equipped with a 20x objective. The software NDP.View2 (version 2.7.25) was used to assess the obtained digital images. Fluorescent microscopic images were obtained with a Zeiss fluorescent microscope and a 5x objective.
Microbleeds and microinfarcts were identified on the H&E-stained sections, using the following criteria. Any area of extravasated (lysed) red blood cells with or without hematoidin was classified as an acute/recent microbleed. A few hemosiderin deposits at the edges of the lesion were allowed. An area with many focal hemosiderin deposits with or without evidence of hematoidin was classified as an old/chronic microbleed^{12 (link)}. Recent/acute microinfarcts were considered areas of tissue pallor with evidence of ‘red’ (i.e. hypoxic) neurons. Old/chronic microinfarcts were characterized by tissue loss with cavitation or ‘puckering’ and GFAP positivity around the edges of the lesion^{5 (link)}. CAA severity was evaluated on the Aβ-stained sections from the pre-defined standard areas of frontal, temporal, parietal, and occipital cortex using a 4-point scale; absent (0), scant Aβ deposition (1), some circumferential Aβ (2), widespread circumferential Aβ (3), following proposed consensus criteria^{13 (link)}. Scores from the four areas were added to form a cumulative CAA severity score (Table 1).
Sholl analysis was performed to assess local CAA burden surrounding microbleeds and microinfarcts, as described previously^{7 (link)}. From the serial sections from three cases, annotated microbleeds and microinfarcts on the H&E-stained sections were included, except when they were located close to another lesion or to an edge of the section. On each section, two control areas were selected on H&E (blinded for CAA severity), in a local area without a lesion. Each lesion or control area was localized on the adjacent Aβ-stained section and images were exported at 2.5x magnification. Each lesion was covered with a round or oval shaped mask in Paint. The same size and shape masks were used for the accompanying control areas to ensure blinding for lesion presence. Sholl analyses were performed >1 week on de-identified images in an in-house developed interface incorporated in MeVisLab (MeVis Medical Solutions AG). Markers were placed in the center of Aβ positive cortical vessels. The cortical ribbon was manually outlined and the resulting area surrounding the masks was divided into four concentric shells (each shell measuring 100 pixels in width, which equals 360 μm). In each shell, the density of Aβ positive cortical vessels / mm² was calculated. Microbleed size was calculated by measuring the greatest diameter on the H&E section that captured the center of the lesion.
From the same serial sections, all microbleeds and microinfarcts that were annotated on the H&E-stained sections were included for the single-vessel analysis. All stained sections (i.e. H&E, Aβ, fibrin(ogen), SMC/Aβ/lectin) surrounding a microbleed or microinfarct were visually inspected to determine whether the presumed ‘culprit’ vessel was visible and to determine presence or absence of Aβ, fibrin(ogen), or SMCs in the wall of the ‘culprit’ vessel at the lesion site or in the same vessel upstream or downstream from the lesion site. Because immunohistochemistry against Aβ was performed on section 2, 6, 10, 14 etc. individual vessels could be traced to determine the presence or absence of Aβ in detail.

van Veluw S.J., Scherlek A.A., Freeze W.M., ter Telgte A., van der Kouwe A.J., Bacskai B.J., Frosch M.P, & Greenberg S.M. (2019). Different microvascular alterations underlie microbleeds and microinfarcts. Annals of neurology, 86(2), 279-292.

Publication 2019

Blood Vessel Cortex, Cerebral Erythrocytes Fibrin Glial Fibrillary Acidic Protein Hematoidin Hemosiderin Hypoxia Immunohistochemistry Lectin Microscopy Neurons Occipital Lobe Ogen Tissues

Bronchoalveolar Lavage for EIPH Assessment

Cited 3 times

Twenty-four hours after the treadmill exercise, BALf was collected; with this aim, horses were restrained in a stock and sedated with detomidine hydrochloride (0.01 mg/kg IV; Domosedan; Vetoquinol, Italy). Airway endoscopy was performed as described above. To perform the BAL, 60 mL of a 0.5% lidocaine hydrochloride solution was sprayed at the level of the tracheal bifurcation in order to inhibit the coughing reflex; then, the endoscope was passed into the bronchial tree until it was wedged firmly within a segmental bronchus. Here, a 300 mL pre-warmed sterile saline 0.9% was instilled, and the fluid was immediately aspirated. The BALf sample was stored in sterile ethylenediaminetetraacetic acid (EDTA) tubes and processed within 90 min. To perform the cytological examination, a few drops of pooled BALf were cytocentrifugated (Rotofix 32, Hettich Cyto System, Tuttlingen, Germany) at 500 rpm for 5 min. The slides were air dried, stained with May-Grünwald Giemsa and Perl’s Prussian blue, and observed under a light microscope at 400× and 1000× for 400-cell leukocyte differential counting [34 (link)]. To evaluate EIPH, 100 macrophages were assessed. The percentage of hemosiderophages on the total of macrophages was calculated and hemosiderin was scored from 0 to 4 based on the grading of blue coloration in the cytoplasm of the macrophages [35 (link)]; then, the percentage of hemosiderophages was multiplied by the median hemosiderin score to obtain a simplified total hemosiderin score (sTHS), with a maximum score of 400 [36 (link)].

Lo Feudo C.M., Stucchi L., Stancari G., Alberti E., Conturba B., Zucca E, & Ferrucci F. (2022). Associations between Exercise-Induced Pulmonary Hemorrhage (EIPH) and Fitness Parameters Measured by Incremental Treadmill Test in Standardbred Racehorses. Animals : an Open Access Journal from MDPI, 12(4), 449.

Publication 2022

Bronchi Cardiac Arrest Cytoplasm detomidine hydrochloride Edetic Acid Endoscopes Endoscopy Equus caballus ferric ferrocyanide Hemosiderin Leukocytes Lidocaine Hydrochloride Light Microscopy Macrophage Reflex Saline Solution Sterility, Reproductive Tertiary Bronchi Trachea Trees

Microbleeds and Stroke Outcomes

Cited 3 times

Request for access to data will be considered by the corresponding author.
We prospectively studied 2156 patients with a probable or definite TIA/ischemic stroke recruited from 2 study centers—OXVASC and The University of Hong Kong (HKU). In brief, OXVASC is an on-going population-based study of all acute vascular events occurring within a predominantly white population of all 92 728 individuals, irrespective of age, who are registered with 100 general practitioners in 8 general practices of Oxfordshire, United Kingdom.^{16 (link)} The analysis herein includes 1080 consecutive cases of TIA/ischemic stroke recruited from November 2004 to September 2014 who had a cerebral magnetic resonance imaging (MRI) incorporating a hemosiderin-sensitive sequence and was subsequently diagnosed to have a TIA/ischemic stroke. The imaging protocol of OXVASC has been described in detail elsewhere.^{17 (link),18 (link)} A further 1076 consecutive patients who were predominantly Chinese with a diagnosis of acute ischemic stroke who received an MRI scan incorporating a hemosiderin-sensitive sequence at the HKU MRI Unit was recruited from March 2008 to September 2014.^{13 (link)} Both cohorts had similar antiplatelet treatment policies, and antiplatelet treatment was started routinely irrespective of microbleed burden. However, patients with a diagnosis of cerebral amyloid angiopathy, defined according to the modified Boston criteria,^{19 (link)} presenting with a transient focal neurological episode,^{20 (link)} were not considered as having TIAs and were not included in this study.
All patients gave written informed consent, or assent was obtained from a relative of patients who were unable to provide consent. The 2 studies were approved by the local research ethics committee.
We collected demographic data, atherosclerotic risk factors, premorbid antithrombotic use, details of hospitalization of index event, and medications on discharge during face-to-face interview and cross-referenced these with primary care and hospital records in both cohorts.
Patients with TIA/ischemic stroke recruited from OXVASC were scanned with a 1.5-T or 3-T MRI scanner. All 1076 HKU patients were scanned using a 3-T MRI scanner. Microbleeds were detected using T2*-weighted gradient-recalled echo (GRE) in OXVASC and using susceptibility weighted imaging (SWI) in HKU. Details of scan parameters are provided in Table I in the online-only Data Supplement.
Two neurologists, supervised by 2 consultant neuroradiologists (H.K.F.M. and W.K.), interpreted all MRIs. Microbleeds were defined according to current guidelines,^{21 (link)} the location scored using the Microbleed Anatomical Rating Scale,^{22 (link)} and burden graded as absent, 1, 2 to 4, and ≥5.^{6 (link)} The intrarater κ for interpretation of microbleed burden in 50 randomly selected scans was 0.88 (OXVASC) and 0.81 (HKU), and the interrater κ was 0.84. White matter hyperintensity severity, enlarged perivascular space burden, and presence of lacunes were also determined based on previously validated scales (online-only Data Supplement).^{23 (link)–25 (link)}All patients in OXVASC were followed-up regularly by a research nurse or physician at 1, 3, 6, 12, 24, 60, and 120 months after the index event. Patients recruited from HKU were followed-up by a clinician every 3 to 6 months or more frequently if clinically indicated. All patients were assessed for the following clinical outcomes: (1) recurrent stroke (ischemic and hemorrhagic), (2) acute coronary events (acute coronary syndrome and sudden cardiac death), (3) major extracranial bleeding, and (4) mortality (vascular and nonvascular). The definition of recurrent stroke required a sudden new neurological deficit fitting the definition of ischemic stroke or ICH, occurring after a period of unequivocal neurological stability and not attributable to cerebral edema, mass effect, or hemorrhagic transformation of the incident cerebral infarction. Modified Rankin Scale (mRS) at 1 month after recurrent stroke was determined and disabling stroke defined as mRS >2 (refer to online-only Data Supplement for definitions of other clinical outcomes). Where needed, details of clinical outcomes were supplemented by medical records from primary care practices, hospitals, as well as the Deaths General Register Office.
We performed an updated systematic review according to the PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) and searched Medline and Embase from April 2015 (date last systematic review^{6 (link)} on this topic was performed) to September 2017 with the following search strategy:
We included published and unpublished studies that fulfilled the following criteria: (1) included a study population of patients with TIA or ischemic stroke, (2) performed MRI T2*-GRE or SWI sequences at baseline to detect presence of microbleeds and had ischemic stroke or ICH as an outcome, (3) had a prospective study design with at least 3 months of follow-up, and (4) subjects were predominantly (≥70% of the study population) on antiplatelet agents.

Lau K.K., Lovelock C.E., Li L., Simoni M., Gutnikov S., Küker W., Mak H.K, & Rothwell P.M. (2018). Antiplatelet Treatment After Transient Ischemic Attack and Ischemic Stroke in Patients With Cerebral Microbleeds in 2 Large Cohorts and an Updated Systematic Review. Stroke, 49(6), 1434-1442.

Publication 2018

Acute Coronary Syndrome Acute Ischemic Stroke Antiplatelet Agents Blood Vessel Brain Perivascular Spaces Cardiac Events Cerebral Amyloid Angiopathy Cerebral Edema Cerebral Hemorrhage Cerebrovascular Accident Chinese Consultant Diagnosis ECHO protocol Ethics Committees, Research Face General Practitioners Hemosiderin Hospitalization Infarction Neurologists Nurses Patient Discharge Patients Pharmaceutical Preparations Physicians Primary Health Care prisma Radionuclide Imaging Stroke, Ischemic Sudden Cardiac Death Susceptibility, Disease Transients White Matter White Person

Hemosiderin Deposition in Brain AVMs

Cited 3 times

Ethics approval was obtained from the Institutional Review Board and all patients provided written informed consent. Unruptured bAVM patients undergoing surgical resection at our institution between August 1992 and January 2016, with pathology specimens and pre-treatment digital subtraction angiography (DSA) available were selected (n=125). Of these, 25 had supratentorial location with large and medium volume (>3.5cc, volume=πxyz/6, where x, y, and z represent the diameter of each axis on angiography).^{6 (link)} Patients were further subdivided into 16 with no pathological evidence of hemosiderin (SIM-) and 9 with hemosiderin in or around the bAVM (SIM+). Hemosiderin positivity was based on a 5-point grading scale ranging from 0 to 4, where 0 indicates no hemosiderin and 1 through 4 indicates the presence of hemosiderin.^{3 (link),7 (link)}Flow analysis software (syngo iFlow®; Siemens) was used to reconstruct color-coded DSA (Figure 1). The diameter of a region of interest (ROI) was the caliber of a selected vessel. ROIs were placed as close to the AVM nidus as possible. For each set of manually drawn references, the time versus intensity graph was produced automatically by the software with the following parameters:
For this project, we focused on measurements of contrast kinetics, both direct (TTP) and indirect (MTT). For AVMs with multiple feeding arteries, the ROI was drawn on the feeding artery (FA) with the largest diameter. For AVMs with multiple draining veins (DV), the longest TTP was selected to best reflect overall venous impedance.^{5 (link)} MTT through the nidus was defined as the time difference between peak contrast density in the FA and DV.^{4 (link)} We also calculated the ratio of TTP DV/FA. iFlow measurements were by consensus of one neurosurgeon and one neurointerventional radiologist.
Patient demographics, clinical presentation, AVM location, flow-related aneurysms, and angioarchitectural characteristics for bAVM patients were collected using standardized definitions (Table).^{8 (link)} Venous pouches (varix) were defined as focal aneurysmal dilatations of the proximal draining vein. Feeding artery enlargement was defined as larger feeding artery when it was moderately to severely enlarged.
We compared characteristics of unruptured bAVMs with and without SIM using two-sided, two-sample t-tests for continuous variables and Fisher’s exact tests for categorical variables. P-values ≤0.05 were considered statistically significant. Statistical analysis was performed using Stata 13.1 (College Station, TX: StataCorp LP).

Chen X., Cooke D.L., Saloner D., Nelson J., Su H., Lawton M.T., Hess C., Tihan T., Zhao Y, & Kim H. (2017). Higher Flow is Present in Unruptured Arteriovenous Malformations with Silent Intralesional Microhemorrhages. Stroke, 48(10), 2881-2884.

Publication 2017

Aneurysm Angiography Angiography, Digital Subtraction Arteries Arteriovenous Malformations, Cerebral Blood Vessel Epistropheus Ethics Committees, Research Hemosiderin Hypertrophy Kinetics Neurosurgeon Operative Surgical Procedures Patients Radiologist Varices Veins

Most recents protocols related to «Hemosiderin»

Quantifying Cerebral Microhemorrhages in Brain Tissue

The brains were sectioned into 20-μm or 40-µm coronal sections with a freezing microtome (Thermo Fisher Scientific, Cleveland, OH, USA). Every seventh section was collected for Prussian blue staining to detect hemosiderin (marker of CMH formation) performed by the Research Services Core (Department of Pathology and Laboratory Medicine at UCI Medical Center). Briefly, sections were stained with freshly prepared 5% potassium hexacyanoferrate trihydrate (Sigma-Aldrich, St. Louis, MO, USA) and 10% hydrochloric acid (Sigma-Aldrich, St. Louis, MO, USA) for 30 min. After rinsing in water, sections were counterstained with nuclear fast red, dehydrated, and coverslipped. To quantify CMH number, CMH were detected and photographed at 20× magnification with a light microscope by a blinded observer. Whole slide images were scanned to quantify the total area of the brain section. The area of the section was analyzed by a blinded observer using National Institute of Health (NIH) ImageJ software 1.52. CMH number was then adjusted to total area of the brain section [13 (link)].

Fang C., Lau W.L., Sun J., Chang R., Vallejo A., Lee D., Liu J., Liu H., Hung Y.H., Zhao Y., Paganini-Hill A., Sumbria R.K., Cribbs D.H, & Fisher M. (2023). Chronic kidney disease promotes cerebral microhemorrhage formation. Journal of Neuroinflammation, 20, 51.

Publication 2023

Brain ferric ferrocyanide ferrocyn Hemosiderin Hydrochloric acid Light Microscopy Microtomy Pharmaceutical Preparations Potassium

Histological Evaluation of Placental Pathology

Tissue samples were obtained from the placenta (at least two samples), umbilical cord (typically three samples), and fetal membranes (at least one sample) at delivery. The samples were fixed in 10% neutral buffered formalin, embedded in paraffin, and sliced into 4-μm sections that were stained with hematoxylin and eosin (H&E) for microscopic assessment by a pathologist who was blinded to the patients’ clinical information. MIR was classified as Stage 1 (acute subchorionitis: patchy, diffuse accumulations of neutrophils in the subchorionic plate and/or membranous chorionic trophoblast layer), Stage 2 (acute chorioamnionitis: several scattered neutrophils in the chorionic plate or membranous chorionic connective tissue and/or the amnion), or Stage 3 (necrotizing chorioamnionitis: degenerating neutrophils, thickened, eosinophilic amniotic basement membrane, and at least focal amnionic epithelial degeneration). FIR was classified as Stage 1 (chorionic vasculitis/umbilical phlebitis: neutrophils in the wall of any chorionic plate vessel or the umbilical vein), Stage 2 (umbilical vasculitis: neutrophils in one or both umbilical arteries, with or without involvement of the umbilical vein), or Stage 3 (necrotizing funisitis or concentric umbilical perivasculitis: neutrophils, cellular debris, eosinophilic precipitates, and/or mineralization arranged in a concentric band, ring, or halo around one or more umbilical vessels). Pathologic features of the placenta that are indicative of maternal vascular malperfusion (MVM) include placental hypoplasia, infarction, retroplacental hemorrhage, distal villous hypoplasia, and accelerated villous maturation. Infarction hematoma was defined as a histologically confirmed hemorrhage encased by infarction [22 (link)]. According to Redline et al., diffuse chorioamniotic hemosiderosis was histologically defined as the diffuse deposition of retractile golden brown hemosiderin crystals in the chorioamniotic layers of the chorionic plate and/or membranes on the H&E stained sections [24 (link)].

Nomiyama M., Nakagawa T., Yamasaki F., Hisamoto N., Yamashita N., Harai A., Gondo K., Ikeda M., Tsuda S., Ishimatsu M., Oshima Y., Ono T., Kozuma Y, & Tsumura K. (2023). Contribution of Fetal Inflammatory Response Syndrome (FIRS) with or without Maternal-Fetal Inflammation in The Placenta to Increased Risk of Respiratory and Other Complications in Preterm Neonates. Biomedicines, 11(2), 611.

Publication 2023

Amnion Blood Vessel Cells Chorioamnionitis Chorion Connective Tissue Eosin Eosinophilia Fetal Membranes Formalin Funisitis Hematoma Hemochromatosis Hemorrhage Hemosiderin hypoplasia Infarction Membrane, Basement Microscopy Mothers Neutrophil Obstetric Delivery Paraffin Embedding Pathologists Patients Phlebitis Physiologic Calcification Placenta Tissue, Membrane Tissues Trophoblast Umbilical Arteries Umbilical Cord Umbilical Vein Umbilicus Vasculitis

Histological Analysis of Endarterectomy Specimens

Immediately after removal, harvested endarterectomy specimens were placed in 10% formaldehyde. Representative parts of the specimen were cross-sectioned in approximately 4 mm thick samples. In further processing, the samples were decalcified by a hydrochloric acid solution and embedded in paraffin. The samples were cut into five-micron-thick tissue sections. Xylene was used as a deparaffinization agent, and graded alcohol was used for the hybridization of the tissue sections. For staining parallel sections, hematoxylin and eosin with the van Gieson/orcein method were used. The indirect immunohistologic method was used for the detection of endothelial cells (CD31 marker, primary mouse anti-human monoclonal antibody and clone JC70A) and macrophages (CD68 marker, primary mouse anti-human monoclonal antibody and clone PG-M1). All histological analyses were performed by one experienced pathologist (VM) using a bright-field optical microscope (Nikon Eclipse E 400).
Endarterectomy specimens were scanned for multiple histological features, including eccentricity, the presence of atheromatous or fibrous tissue, calcification, myxoid change, hemorrhage, thrombosis, inflammation, foamy macrophage, giant cell reaction, hemosiderin, neovascularisation or ossification (TAB 1). All specimens were divided into AHA groups IV/V, VIII, or VI, according to the AHA classification [9 (link)]. Plaques in the AHA VI group were gathered in the group of unstable plaques.

Svoboda N., Kočí K., Seidlová A., Mandys V., Suttnar J., Hlaváčková A., Kučerka O., Netuka D, & Malý M. (2023). Oxidative Stress as a Reliable Biomarker of Carotid Plaque Instability: A Pilot Study. Antioxidants, 12(2), 506.

Publication 2023

Antibodies, Anti-Idiotypic Atheroma Clone Cells Crossbreeding Endarterectomy Endothelial Cells Eosin Ethanol Fibrosis Foam Cells Formaldehyde Granuloma, Foreign-Body Hematoxylin Hemorrhage Hemosiderin Homo sapiens Hydrochloric acid Inflammation Light Microscopy Macrophage Monoclonal Antibodies Mus orcein Osteogenesis Paraffin Embedding Pathologic Neovascularization Pathologists Physiologic Calcification Senile Plaques Thrombosis Tissues Xylene

Cerebral Cavernous Malformation Hemorrhage Classification

Cited 1 time

Haemorrhages were classified in accordance with reporting standards from the Angioma Alliance [12 (link)], with a symptomatic haemorrhage defined as radiological (CT or MRI) evidence of acute haemorrhage, associated with acute or subacute clinical symptoms. ‘Interval change’ during follow-up was defined as a change in signal intensity on T2-weighted MRI scan, without symptoms suggestive of haemorrhage. Size was measured as maximum diameter including surrounding hemosiderin on T2-weighted, 1.5- to 3-T MRI imaging, as described previously [8 (link)]. If multiple intracranial lesions were present, for the per-patient analysis, we used the median diameter of combined cavernoma. If no genetic testing was completed, a familial cerebral cavernous malformation (FCCM) was defined by established criteria as the presence of both: diffuse CCM (five or more) or occurrence of CCM in at least two first-degree family members. In patients with genetic testing available, FCCM was defined as confirmation of one of three genetic mutations known to be associated with FCCM (CCM1, CCM2, and CCM3) [13 , 14 ]. Follow-up duration was defined as the time in months from initial diagnosis until last clinic review with a neurosurgeon.

Gillespie C.S., Alnaham K.E., Richardson G.E., Mustafa M.A., Taweel B.A., Islim A.I., Hannan C.J, & Chavredakis E. (2023). Predictors of future haemorrhage from cerebral cavernous malformations: a retrospective cohort study. Neurosurgical Review, 46(1), 52.

Publication 2023

Angioma Cerebral Cavernous Malformations 2 Cerebral Cavernous Malformations 3 Diagnosis Familial cerebral cavernous malformation Family Member Hemangioma, Cavernous Hemorrhage Hemosiderin KRIT1 protein, human MRI Scans Mutation Neurosurgeon Patients X-Rays, Diagnostic

Reducing Autofluorescence in FFPE Brain Tissue

Formalin-fixed paraffin embedded (FFPE) mouse brains were sectioned (8-μm thickness) and glass mounted. To reduce the autofluorescence signals by greater than 90% intensity (e.g., lipofuscin or hemosiderin), FFPE slides were photobleached up to 48 h using a multispectral LED array in the cold room overnight to reduce the autofluorescence in the brain tissue.^{75 (link)} The sections were deparaffinized, PBS-washed and stained with 25 μM for 30 min. The sections were washed with PBS buffer and a coversliped with PermaFluor (Thermo) as the mounting media. For FFPE human brain sections, the same procedures were followed. For EMBER data collection, the same steps were taken without the autofocus function and with zoom of 1.5.

Yang H., Yuan P., Wu Y., Shi M., Caro C.D., Tengeiji A., Yamanoi S., Inoue M., DeGrado W.F, & Condello C. (2023). EMBER multi-dimensional spectral microscopy enables quantitative determination of disease- and cell-specific amyloid strains. bioRxiv.

Publication Preprint 2023

Brain Buffers Common Cold Formalin Hemosiderin Homo sapiens Lipofuscin Mice, Laboratory Paraffin Tissues

Top products related to «Hemosiderin»

Hydrochloric acid (hcl) by Merck Group

Sourced in Germany, United States, United Kingdom, India, Italy, France, Spain, Australia, China, Poland, Switzerland, Canada, Ireland, Japan, Singapore, Sao Tome and Principe, Malaysia, Brazil, Hungary, Chile, Belgium, Denmark, Macao, Mexico, Sweden, Indonesia, Romania, Czechia, Egypt, Austria, Portugal, Netherlands, Greece, Panama, Kenya, Finland, Israel, Hong Kong, New Zealand, Norway

Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.

Nuclear fast red by Merck Group

Sourced in United States, Germany, Italy, Netherlands

Nuclear Fast Red is a dye used in histology and cytology laboratory procedures. It is a bright red-colored dye that is commonly used to stain nuclei in tissue sections or cell preparations. The dye binds to the DNA and RNA within the cell nucleus, allowing for the visualization and identification of cellular structures.

Potassium ferrocyanide by Merck Group

Sourced in United States, Germany, United Kingdom, Canada, Belgium, Australia, Italy, Romania

Potassium ferrocyanide is a chemical compound with the formula K4[Fe(CN)6]. It is a yellow crystalline solid that is commonly used in various laboratory applications. The compound's core function is to serve as a reagent for the detection and analysis of various ions, particularly iron and copper ions. It can also be used as a component in the preparation of other chemical compounds.

Permafluor by Thermo Fisher Scientific

Sourced in United States, Germany, Japan, Italy

PermaFluor is a laboratory equipment product designed for fluorescence-based applications. It provides a stable and consistent fluorescence signal for reliable and reproducible results.

Bx51 microscope by Olympus

Sourced in Japan, United States, Germany, Italy, Denmark, United Kingdom, Canada, France, China, Australia, Austria, Portugal, Belgium, Panama, Spain, Switzerland, Sweden, Poland

The BX51 microscope is an optical microscope designed for a variety of laboratory applications. It features a modular design and offers various illumination and observation methods to accommodate different sample types and research needs.

Potassium hexacyanoferrate trihydrate by Merck Group

Sourced in United States, Germany

Potassium hexacyanoferrate trihydrate is a chemical compound with the formula K3[Fe(CN)6]·3H2O. It is a crystalline, yellow-to-orange solid that is soluble in water. The compound is commonly used in various applications, including chemical analysis, electroplating, and as a pigment in paints.

Permount by Thermo Fisher Scientific

Sourced in United States, Canada, Japan, Germany, United Kingdom, Gabon, China

Permount is a mounting medium used in microscopy to permanently mount specimens on glass slides. It is a solvent-based, xylene-containing solution that dries to form a clear, resinous film, securing the specimen in place and providing optical clarity for microscopic examination.

Vectastain abc kit by Vector Laboratories

Sourced in United States, Canada, United Kingdom, Germany, Japan, France

The Vectastain ABC kit is a product by Vector Laboratories that is used for the detection of specific target antigens in tissue or cell samples. The kit includes reagents necessary for the avidin-biotin complex (ABC) method of immunohistochemistry. The core function of the Vectastain ABC kit is to provide a reliable and sensitive tool for the visualization of target molecules within a sample.

Nuclear fast red aluminium sulphate solution by Carl Roth

Sourced in Germany

Nuclear fast red aluminium sulphate solution is an aqueous solution used as a staining agent in histological and cytological applications. It is primarily utilized for the visualization and differentiation of cellular structures in microscopic preparations.

Roti histokitt by Carl Roth

Sourced in Germany

Roti-Histokitt is a set of reagents designed for the preparation and staining of histological samples. The kit includes solutions for fixation, dehydration, clearing, and embedding of tissue samples. It provides the necessary tools for the essential steps in histological sample preparation.

What is hemosiderin and why is it important?

Hemosiderin is an iron-containing pigment that is formed from the breakdown of hemoglobin. It is found in various tissues, especially in the liver, spleen, and bone marrow, and is a marker of iron overload conditions. The accurate identification and quantification of hemosiderin is crucial for the diagnosis and management of conditions like hemochromatosis, chronic liver disease, and certain hematological disorders.

How can PubCompare.ai help with hemosiderin research?

PubCompare.ai's AI-driven platform can assist researchers in optimizing their hemosiderin research in several ways. First, it allows you to screen protocol literature more efficiently, helping you locate the best protocols from literature, preprints, and patents. Second, the platform's AI analysis can help you identify critical insights, such as key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. This can greatly enhance your research on hemosiderin and related conditions.

What are the different variations or types of hemosiderin?

Hemosiderin can be found in various forms and depositions within the body. It can accumulate in different tissues, such as the liver, spleen, and bone marrow, and its presence can be associated with a variety of clinical conditions. While the basic structure of hemosiderin remains the same, the specific patterns and locations of its deposition can vary depending on the underlying medical condition, which is important to consider when diagnosing and treating these disorders.

What are some common usage challenges with hemosiderin?

One of the main challenges with hemosiderin is accurately identifying and quantifying its presence, as it can be difficult to distinguish from other iron-containing compounds. Additionally, the deposition of hemosiderin can be influenced by a variety of factors, such as the individual's overall iron status, the presence of underlying medical conditions, and the specific tissues affected. Proper sample collection, storage, and analysis techniques are crucial to ensure reliable and reproducible results when measuring hemosiderin levels.

How can PubCompare.ai help researchers overcome these challenges?

PubCompare.ai's AI-driven platform can be a valuable tool for researchers facing challenges with hemosiderin analysis and quantification. The platform allows you to easily locate the best protocols from literature, preprints, and patents, while comparing them to enhance reproducibility and accuracy. By leveraging the platform's AI-driven analysis, you can identify the most effective protocols related to hemosiderin for your specific research goals, helping you overcome common usage challenges and obtain reliable, high-quality data. This can be particularly useful when dealing with the complexities of hemosiderin deposition and measurement.

Hemosiderin

Most cited protocols related to «Hemosiderin»

Most recents protocols related to «Hemosiderin»

Top products related to «Hemosiderin»

More about "Hemosiderin"