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Hepatocyte Growth Factor

Hepatocyte Growth Factor (HGF) is a multifunctional cytokine that plays a crucial role in diverse biological processes, including cell proliferation, motility, and morphogenesis.
HGF is secreted by mesenchymal cells and binds to its receptor, c-Met, expressed on epithelial cells.
This interaction triggers signaling cascades that regulate tissue regeneration, organ development, and wound healing.
HGF has been extensively studied for its potential therapeutic applications in liver disease, kidney injury, and cardiovascular disorders.
Reseachers can discover the power of HGF research protocols with PubCompare.ai, an AI-driven platform that helps locate the best protocols from literature, pre-prints, and patents, while providing tailored comparisons to optimize reproducibility.
Experience seamless research with their user-friendly tools and take your HGF studies to new heights.

Most cited protocols related to «Hepatocyte Growth Factor»

1
Isolation and Differentiation of Human Myoblasts
Cited 74 times
Human myoblasts were isolated from biopsies and cultivated as described previously [19 (link)] in a growth medium consisting of 199 medium and DMEM (Invitrogen Carlsbad, CA) in a 1:4 ratio, supplemented with 20% FCS (Invitrogen), 2.5 ng/ml hepatocyte growth factor (Invitrogen), 0.1 μmol/l dexamethasone (Sigma-Aldrich, St. Louis, MO, USA) and 50 μg/ml gentamycin (Invitrogen). The myogenic purity of the populations was monitored by immunocytochemistry using desmin as marker. Enrichment of myogenic cells was performed using an immunomagnetic cell sorting system (MACS; Miltenyi Biotec, Paris, France) according to the manufacturer's instructions. Briefly, cells were labeled with anti-CD56 (a specific marker of myoblasts) microbeads, and then separated in a MACS column placed in a magnetic field. Purification was checked by immunochemistry using a desmin marker. Differentiation was induced at confluence by replacing the growth medium with DMEM supplemented with 100 μg/ml transferrin, 10 μg/ml insulin and 50 μg/ml of gentamycin (Sigma-Aldrich).
Mamchaoui K., Trollet C., Bigot A., Negroni E., Chaouch S., Wolff A., Kandalla P.K., Marie S., Di Santo J., St Guily J.L., Muntoni F., Kim J., Philippi S., Spuler S., Levy N., Blumen S.C., Voit T., Wright W.E., Aamiri A., Butler-Browne G, & Mouly V. (2011). Immortalized pathological human myoblasts: towards a universal tool for the study of neuromuscular disorders. Skeletal Muscle, 1, 34.
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Publication 2011
Biopsy Cells Culture Media Desmin Dexamethasone Gentamicin Hepatocyte Growth Factor Homo sapiens Immunocytochemistry Insulin Magnetic Fields Microspheres Myoblasts Myogenesis Population Group Transferrin
2
Cryo-EM Workflow for Macromolecular Complexes
Cited 28 times

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Publication 2015
Cardiac Arrest Electron Microscopy Electrons Gold Hepatocyte Growth Factor RAG2 protein, human
3
Differentiation of hESCs into Insulin-Producing Cells
Cited 16 times
hESCs were cultured and passaged as reported elsewhere [10 (link)]. The differentiation of hESCs into INS+ cells was performed using several different protocols. Adherent, flat culture differentiations based on the work of D’Amour et al. [11 (link)] and Kroon et al. [2 (link)] (referred to as ‘flat cultures’). Spin EB differentiations (referred to as ‘spin EBs’) [5 (link)], were set up in APEL medium [6 (link)]. Differentiation of spin EBs under pancreatic-specific conditions was as follows. EBs were formed by the forced aggregation of 2,000 (HES3) or 3,500 (MEL1) hESCs in APEL (the protein-free hybridoma medium component was omitted from this formulation) containing 10 ng/ml bone morphogenetic protein 4 (BMP4) and 150–200 ng/ml activin A (batch dependent) in low-attachment 96-well plates. After 3 days, medium was replaced with APEL containing 200–400 ng/ml noggin (batch dependent). At day 6, medium was replaced with APEL containing 1 × 10−5 mol/l retinoic acid (RA). At day 9, the medium was changed to APEL without polyvinyl alcohol (AEL) containing 1 × 10−5 mol/l RA, 100 μmol/l glucagon-like peptide 1 (GLP1), 1 × B27 and 10 mmol/l nicotinamide. At day 15 of differentiation, EBs were transferred to gelatinised, adherent 96-well plates, and insulin production was induced in AEL containing 10 mmol/l nicotinamide and 50 ng/ml IGF-I. With this system, most EBs contained INS-GFP+ cells by day 30 of differentiation. In addition, INS-GFP+ cells were also differentiated according to a protocol developed by Nostro and colleagues [12 (link)], referred to as the ‘Nostro protocol’. Recombinant human activin A, fibroblast growth factor 10 (FGF10), fibroblast growth factor 7 (KGF), IGF-I and hepatocyte growth factor (HGF) were purchased from R&D Systems (Minneapolis, MN, USA). Basic FGF (FGF2) was purchased from Peprotech (Rocky Hill, NJ, USA). Wingless-type MMTV integration site family, member 3A (WNT3A) and noggin were purchased from R&D Systems or provided by the Australia Stem Cell Centre (Melbourne, VIC, Australia). KAAD-cyclopamine was purchased from Toronto Research Chemicals (North York, ON, Canada); all-trans RA, nicotinamide, SB431542 and GLP1 were purchased from Sigma-Aldrich (St Louis, MO, USA).
Micallef S.J., Li X., Schiesser J.V., Hirst C.E., Yu Q.C., Lim S.M., Nostro M.C., Elliott D.A., Sarangi F., Harrison L.C., Keller G., Elefanty A.G, & Stanley E.G. (2011). INSGFP/w human embryonic stem cells facilitate isolation of in vitro derived insulin-producing cells. Diabetologia, 55(3), 694-706.
Publication 2011
3-keto-N-aminoethylaminoethylcaproyldihydrocinnamoyl cyclopamine 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide activin A Bone Morphogenetic Protein 4 Cells FGF7 protein, human FGF10 protein, human Fibroblast Growth Factor 2 Glucagon-Like Peptide 1 Hepatocyte Growth Factor Homo sapiens Human Embryonic Stem Cells Hybridomas IGF1 protein, human Insulin Mouse mammary tumor virus Niacinamide noggin protein Pancreatic Diseases Polyvinyl Alcohol Proteins Stem Cells Tretinoin
4
Comparative Analysis of PET Image Reconstruction Algorithms
Cited 14 times
PET images were reconstructed using three different algorithms, each of which used the CT scan for attenuation correction and the same normalisation correction factors with scatter and randoms corrected as has been previously described (19 ,20 ,21 ). The standard PET reconstruction algorithm used at our centre is ToF OSEM (VPFX, GE Healthcare), used with 2i, 24ss and 6.4mm filter. The sinograms generated at the time of scanning were retrospectively processed using a ToF OSEM PSF protocol (3i, 24ss, 2mm filter) and the new Q.Clear reconstruction algorithm for penalization factors (betas): 200, 300, 400 and 500.
Visual analyses of the OSEM, OSEM PSF, and Q.Clear PET images, six reconstructions per case, were performed by two consultants (designated Scorer 1 and 2 respectively) with double accreditation in clinical radiology and nuclear medicine, and 11 and 3 years consultant experience respectively. Images were viewed on a GE Advantage Workstation (AW4.6, GE Healthcare). The reconstructions were labelled A to F in a randomised order, with the CT component available for image fusion. Cases were reviewed sequentially, and the reconstructions were ranked (from 1 to 6) according to six image quality (IQ) parameters: overall IQ (1 – excellent, 5 – worst), background liver IQ (1 – excellent, 5 – worst), background mediastinum IQ (1 – excellent, 5 – worst), background marrow IQ (1 – excellent, 5 – worst), noise level (1 – minimal, 5 – unacceptable), and lesion detectability (1 – excellent, 5 – poor).
Scorers also indicated their most and least preferred reconstruction for each case. Inter-rater agreement on ranking within each of the six IQ parameters was assessed using Cohen’s kappa statistic. The proportions of the highest and lowest ranked reconstructions were calculated for each parameter. Alongside the highest frequencies of the most and least preferred reconstruction indicated by the scorers, scores by both scorers for all parameters across the cases were summated for each reconstruction to confirm this quantitatively.
Statistical analyses were performed using IBM SPSS Statistics 22.0 (IBM Corporation, New York, USA). Kappa values were interpreted using the guidelines laid out by Landis and Koch (22 (link)).
Teoh E.J., McGowan D.R., Macpherson R.E., Bradley K.M, & Gleeson F.V. (2015). Phantom and clinical evaluation of the Bayesian penalized likelihood reconstruction algorithm Q.Clear on an LYSO PET/CT system. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 56(9), 1447-1452.
Publication 2015
Consultant Hepatocyte Growth Factor Liver Marrow Mediastinum Radiography Radionuclide Imaging Reconstructive Surgical Procedures X-Ray Computed Tomography
5
Pluripotent Stem Cell Differentiation to Hepatic and Pancreatic Endoderm
Cited 9 times
hESCs (H9 from WiCell, Maddison, WI, USA) and hIPSCs (BBHX8, A1ATD-1, JRO1D; University of Cambridge, Cambridge, UK) [16 (link)] were passaged weekly using collagenase IV and maintained in chemically defined medium (CDM) supplemented with activin A (10 ng/ml) and FGF2 (12 ng/ml), as described previously [17 (link)]. Differentiation was carried out as described in Fig. 1 and is explained in detail in the electronic supplementary material (ESM) Methods.

Protocols to generate hepatic and pancreatic endoderm from hESCs and hIPSCs. Successive culture conditions driving differentiation of pluripotent stem cells towards pancreatic endoderm and hepatic endoderm. A, activin; ADV, advanced DMEM; B, BMP; CMRL, Connaught Medical Research Laboratories medium; CYCP, cytochrome P450; DAPT, N-(N-[3,5-diflurophenylacetyl]-l-alanyl)-S-phenylglycine t-butyl ester; F, FGF; HGF, hepatocyte growth factor; Ly, LY294002; Nog, noggin; OSM, oncostatin M

Cho C.H., Hannan N.R., Docherty F.M., Docherty H.M., Joåo Lima M., Trotter M.W., Docherty K, & Vallier L. (2012). Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia, 55(12), 3284-3295.
Publication 2012
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol activin A Activins alpha 1-Antitrypsin Deficiency Collagenase Cytochrome P450 Endoderm Esters Fibroblast Growth Factor 2 Hepatocyte Growth Factor Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells LY 294002 noggin protein OSM protein, human Pancreas Pluripotent Stem Cells

Most recents protocols related to «Hepatocyte Growth Factor»

1
Cytokine Profiling for Vascular Remodeling
All serum samples, just after centrifugation at 3000 rpm for 10 min, and all CSF samples were immediately stored at − 80 °C until cytokine analysis, other than IL-6. The serum and CSF IL-6 levels were measured on a single detection immediately after centrifugation at room temperature using the electrochemiluminescence immunoassay according to the manufacturer’s instruction (Roche Diagnostics K.K., Tokyo, Japan). The serum cytokine concentrations relevant to the blood vessel regeneration were measured using the MILLIPLEX® (Merck Millipore, Darmstadt, Germany) human angiogenesis/growth factor magnetic bead panel 1 with a single detection, according to the manufacturer's instruction. Fluorescence intensity from the immunoassay was acquired and analyzed using xPONENT 4.2 Software (Luminex Corporation, Austin, TX, USA). The measured cytokines relevant to the vascular remodeling included epidermal growth factor, angiopoietin 2, granulocyte-colony stimulating factor (G-CSF), bone morphogenetic protein-9 (BMP-9), endoglin, leptin, hepatocyte growth factor (HGF), placental growth factor, vascular endothelial growth factor (VEGF)-C, VEGF-D, fibroblast growth factor-2 (FGF-2), and VEGF-A. Values under the dynamic range were replaced by half of the lower sensitivity limit.
Masuda H., Mori M., Uzawa A., Uchida T., Muto M., Ohtani R., Aoki R, & Kuwabara S. (2023). Elevated serum levels of bone morphogenetic protein-9 are associated with better outcome in AQP4-IgG seropositive NMOSD. Scientific Reports, 13, 3538.
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Publication 2023
Angiogenesis Factor Angiopoietin-2 austin Blood Vessel Centrifugation Cytokine Diagnosis Endoglin Epidermal growth factor Fibroblast Growth Factor 2 Fluorescence Granulocyte Colony-Stimulating Factor Growth Differentiation Factor 2 Growth Factor, Placenta Hepatocyte Growth Factor Homo sapiens Hypersensitivity Immunoassay Leptin Regeneration Serum Vascular Endothelial Growth Factor D VEGFC protein, human VEGF protein, human
2
Adipose-Derived Stem Cell Secretome Analysis
ADSC were cultivated in cell culture medium according to the experimental groups until 90% confluence. Cell layers were washed with PBS and incubated in MEM α without supplements for 24 h at 37 °C, 5% CO₂. Supernatant was collected and immediately stored at −80 °C. Samples were analysed by ELISA Quantibody (Testing Service by RayBiotech Inc., Peachtree Corners, GA, United States) for Hepatocyte Growth Factor (HGF), Vascular Endothelial Growth Factor (VEGF), Vascular Endothelial Growth Factor-Receptor 2 (VEGF-R2), Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF), Endothelial Growth Factor Receptor (EGF-R), Fibroblast Growth Factor 4 (FGF-4), Macrophage Colony Stimulating Factor (MCSF), Macrophage Colony Stimulating Factor Receptor (MCSF-R), Stem Cell Factor (SCF), Stem Cell Factor Receptor (SCF-R), Intercellular Adhesion Molecule 1 (ICAM-1), Monocyte Chemoattractant Protein 1 (MCP-1) and Chemokine Ligand 5 (CCL5, RANTES).
Hermann M., Peddi A., Gerhards A., Schmid R., Schmitz D., Arkudas A., Weisbach V., Horch R.E, & Kengelbach-Weigand A. (2023). Secretome of Adipose-Derived Stem Cells Cultured in Platelet Lysate Improves Migration and Viability of Keratinocytes. International Journal of Molecular Sciences, 24(4), 3522.
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Publication 2023
CCL5 protein, human Cell Culture Techniques Cells Chemokine Culture Media Dietary Supplements Endocrine Glands Endothelial Growth Factor Receptor Enzyme-Linked Immunosorbent Assay FGF4 protein, human Gene, c-fms Hepatocyte Growth Factor Intercellular Adhesion Molecule-1 Ligands Macrophage Colony-Stimulating Factor Monocyte Chemoattractant Protein-1 R Factors Stem Cell Factor Stem Cell Factor Receptor Stem Cells Vascular Endothelial Growth Factor Receptor-2 Vascular Endothelial Growth Factors
3
Comprehensive Gene Expression Analysis of rBMSC
Total RNA from rBMSC cells, either on the sheets or in cell suspension, was extracted using TRIzol reagent (Thermo Fisher Scientific) and PureLink RNA Mini Kt (Life Technologies) according to the manufacturer’s protocols. cDNA was prepared from 1 µg of total RNA using high capacity cDNA reverse transcription kits (Life Technologies). Real time PCR analysis for rat ß-actin (Rn0066869_m1), vascular endothelial growth factor (VEGF, Rn01511602_m1), fibroblast growth factor 2 (FGF2, Rn00570809_m1), insulin-like growth factor-1 (IGF-1, Rn00710306_m1), and hepatocyte growth factor (HGF, Rn00566673_m1) was performed with the TagMan Universal PCR Master Mix using an Applied Biosystems Step One instrument (Applied BiosystemsTM, Foster City, CA, USA). Samples were run as triplicates in separate tubes to permit quantification of the target gene normalized to ß-actin. Sequences of primers used for this assay were manufactured by Applied BiosystemsTM.
Total RNA from kidney cortex tissue of each rat was isolated using TRIzol Reagent (Thermo Fisher Scientific). Real-time RT-PCR was performed using the superscript III first-strand synthesis kit, the power SYBR green PCR master mix (Thermo Fisher Scientific), and the ABI 7900 Sequence Detection System (Applied Biosystems), as described previously [46 (link)]. Samples were run as triplicates in separate tubes to permit quantification of the target molecules normalized to ß-actin, and analyzed by 2(−∆∆Ct). The relative mRNA levels of the target molecules were expressed relative to the normal control kidney, which was set at unity. Sequences of primers used for rat TGFß1, PAI-1, FN, Col I, and ß-actin were described previously [45 (link),47 (link)]. Sequences of new primers, related to regenerative genes such as VEGF, FGF2, HGF, IGF-1, and Pax2, cellular oxidative stress and inflammation such as vanin-1, IL-10, and cellular apoptosis such as TSP-1, Bcl-2, and HO-1 are included in the Supplementary Table S1.
Wang B., Kim K., Tian M., Kameishi S., Zhuang L., Okano T, & Huang Y. (2023). Engineered Bone Marrow Stem Cell-Sheets Alleviate Renal Damage in a Rat Chronic Glomerulonephritis Model. International Journal of Molecular Sciences, 24(4), 3711.
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Publication 2023
Actins Anabolism Apoptosis BCL2 protein, human Biological Assay Cells DNA, Complementary Fibroblast Growth Factor 2 Genes Hepatocyte Growth Factor IGF1 protein, human IL10 protein, human Inflammation Kidney Kidney Cortex Oligonucleotide Primers Oxidative Stress PAX2 protein, human Plasminogen Activator Inhibitor 1 Real-Time Polymerase Chain Reaction Regeneration Reverse Transcription RNA, Messenger SYBR Green I Thrombospondin 1 Tissues trizol Vascular Endothelial Growth Factors
4
Quantifying Gene Expression in Ulcerative Colitis
Total RNA was extracted from biopsy specimens of UC patients and murine colorectal tissue using RNeasy Mini kit (Qiagen, Hilden, Germany). TaqMan real-time PCR was performed as previously described37 (link). All gene expression was normalized to 18S ribosomal RNA. The following primer sets (Thermo Fisher Scientific, Waltham, MA) were used: human urokinase-type plasminogen activator (PLAU/uPA, Hs01547054_m1), matrix metalloproteinase-8 (MMP-8, Hs01029057_m1), plasminogen (PLG, Hs00264877_m1), hepatocyte growth factor (HGF, Hs00300159_m1), endoglin (ENG, Hs00923996_m1), 18S (Hs99999901_s1), urokinase-type plasminogen activator receptor (PLAUR, Hs00959822_m1), murine urokinase-type plasminogen activator (Plau/uPA, Mm00447054_m1), urokinase-type plasminogen activator receptor (Plaur, Mm00440913_m1), RANTES (Ccl5, Mm01302427_m1), and Rn18s (Mm03928990_g1).
Kida Y., Okahisa T., Sato Y., Bando M., Fujimoto S., Ma B., Nakagawa T., Kawaguchi T., Nakamura F., Okamoto K., Miyamoto H., Sogabe M., Tsuneyama K, & Takayama T. (2023). Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice. Scientific Reports, 13, 2899.
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Publication 2023
Biopsy CCL5 protein, human Endoglin Gene Expression Hepatocyte Growth Factor Homo sapiens Mus Neutrophil Collagenase Oligonucleotide Primers Patients Plasminogen PLAU protein, human PLAUR protein, human Real-Time Polymerase Chain Reaction RNA, Ribosomal, 18S Tissues
5
Cytokine Dynamics After Surgery
Serum concentrations of IL-6, tumor necrosis factor-α (TNF-α), and hepatocyte growth factor (HGF) were measured at 1, 4, 6, and 24 h after surgery using ELISA kits (R&D Systems, Minneapolis, MN, United States). IL-6 concentration in the RML tissue was also quantified 1 h after surgery.
Masuo H., Shimizu A., Motoyama H., Kubota K., Notake T., Yoshizawa T., Hosoda K., Yasukawa K., Kobayashi A, & Soejima Y. (2023). Impact of endothelial nitric oxide synthase activation on accelerated liver regeneration in a rat ALPPS model. World Journal of Gastroenterology, 29(5), 867-878.
Publication 2023
Enzyme-Linked Immunosorbent Assay Hepatocyte Growth Factor Operative Surgical Procedures Serum Tissues TNF protein, human

Top products related to «Hepatocyte Growth Factor»

1
Hepatocyte growth factor (hgf) by Thermo Fisher Scientific
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The HGF is a laboratory equipment product designed for scientific research and analysis. It functions as a device for the measurement and detection of specific analytes within a sample. The core function of the HGF is to provide accurate and reliable data to support various scientific investigations and experiments.
2
Dexamethasone (dex) by Merck Group
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
3
Epidermal growth factor (egf) by Thermo Fisher Scientific
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EGF is a lab equipment product from Thermo Fisher Scientific. It is a recombinant human Epidermal Growth Factor (EGF) protein. EGF is a growth factor that plays a role in cell proliferation and differentiation.
4
Elisa kit by R&D Systems
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ELISA kits are laboratory tools used to detect and quantify specific proteins or other molecules in a sample. The kits utilize enzyme-linked immunosorbent assay (ELISA) technology to identify the target analyte. ELISA kits provide a standardized and reliable method for sample analysis.
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Hepatocyte growth factor (hgf) by Merck Group
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HGF is a laboratory equipment product developed by Merck Group. It is designed for research applications that require the measurement and analysis of hepatocyte growth factor (HGF) levels. The core function of HGF is to facilitate the quantitative detection and evaluation of HGF in biological samples.
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Nicotinamide by Merck Group
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Nicotinamide is a form of vitamin B3 that serves as a precursor for the coenzyme nicotinamide adenine dinucleotide (NAD) in biological systems. NAD is essential for various metabolic processes within cells, including energy production and DNA repair.
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Basic fibroblast growth factor (bfgf) by Thermo Fisher Scientific
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The BFGF is a laboratory instrument designed for the controlled growth and expansion of cells. It provides a regulated and consistent environment for cell culture applications. The core function of the BFGF is to maintain optimal temperature, humidity, and gas composition to support the proliferation and differentiation of cells.
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Hepatocyte growth factor (hgf) by R&D Systems
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HGF is a recombinant human protein. It functions as a growth factor.
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Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.

More about "Hepatocyte Growth Factor"

Hepatocyte Growth Factor (HGF), also known as Scatter Factor (SF), is a crucial multifunctional cytokine that plays a pivotal role in diverse biological processes.
It is secreted by mesenchymal cells and binds to its receptor, c-Met, which is expressed on epithelial cells.
This interaction triggers signaling cascades that regulate tissue regeneration, organ development, and wound healing.
HGF has been extensively studied for its potential therapeutic applications in liver disease, kidney injury, and cardiovascular disorders.
Researchers can discover the power of HGF research protocols with PubCompare.ai, an AI-driven platform that helps locate the best protocols from literature, preprints, and patents, while providing tailored comparisons to optimize reproducibility.
This user-friendly tool can enhance your HGF studies by providing access to a wide range of relevant protocols, including those involving Dexamethasone, Epidermal Growth Factor (EGF), ELISA kits, Nicotinamide, Basic Fibroblast Growth Factor (BFGF), Fetal Bovine Serum (FBS), and TRIzol reagent.
These protocols can be seamlessly integrated into your research, allowing you to explore the multifaceted roles of HGF and its potential therapeutic applications.
Experience the benefits of PubCompare.ai's comprehensive approach to HGF research, where you can discover the latest advancements, optimize your experimental design, and take your studies to new heights.
Explore the platform now and unlock the full potential of Hepatocyte Growth Factor in your research endeavors.