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Histone H2b

Histone H2b is a core histone protein that plays a crucial role in the organization and regulation of eukaryotic chromatin.
It is involved in the compaction of DNA into nucleosomes, the fundamental units of chromatin.
Histone H2b is essential for maintaining chromatin structure and participates in various cellular processes, such as transcription, DNA repair, and epigentetic modifications.
Understanding the functions and dynamics of Histone H2b is crucial for advancing research in areas like gene expression, cellular differentiation, and chromatin-based diseases.
The PubCompare.ai platform can help streamlien your Histone H2b research by providing AI-driven comparisons of published literature, preprints, and patents, allowing you to identify the most reproducible and accurate approaches for your experiments.
Improve your results and enhance your Histone H2b research with the powerful tools offered by PubCompare.ai.

Most cited protocols related to «Histone H2b»

1
Genetic Strains for C. elegans Imaging
Cited 31 times
In this study, wild type indicates one of several integrated transgenic strains. The integrated GFP∷tubulin strain WH204 (Strome et al., 2001 (link)) was used for the studies shown in Figs. 1 and 3 and Tables II and III. The integrated GFP∷tubulin strain AZ244 (Praitis et al., 2001 (link)) was consistently brighter than WH204 and was used for the studies shown in Figs. 5, 6, and 7. The integrated GFP∷histone H2b strain AZ212 (Praitis et al., 2001 (link)) was used in Fig. 5. The integrated GFP∷tubulin, mCherry∷histone strain, OD57, was the wild type used for Fig. 4. OD57 was generated by particle bombardment of a pie-1 promoter vector (Praitis et al., 2001 (link)) engineered to express a histone H2b fusion to a version of mCherry (Shaner et al., 2004 (link)) that included four C. elegans introns and C. elegans preferred codons. This modified mCherry DNA was synthesized by Molecular Cloning Laboratories. The mCherry∷histone strain was crossed with the GFP∷tubulin- expressing strain, OD4, to obtain OD57. mei-2(ct98) worms were obtained from P. Mains (University of Calgary, Calgary, Canada) and were crossed with WH204 to obtain mei-2(ct98) worms expressing GFP∷tubulin, with AZ212 to obtain mei-2(ct98) worms expressing GFP∷histone H2b and with OD57 to obtain mei-2(ct98) worms expressing both GFP∷tubulin and mCherry∷histone. The mei-1(null) allele used in this study was the nonsense mutant mei-1(ct46ct101) (Clark-Maguire and Mains, 1994b (link)). Homozygous mei-1(ct46ct101) worms expressing GFP∷tubulin and used for the studies shown in Table III were described previously (Yang et al., 2003 (link)). For data shown in Fig. 6, homozygous mei-1(ct46ct101) worms expressing a higher level of GFP∷tubulin were derived from crosses between HR1069 (unc-13[e10910] daf-8[e1393] mei-1[ct46ct101]/hT2[bli-4{e937} let{h661}] I; hT2/+ III] and AZ244. The GFP∷MEI-1-expressing strain was EU1065 (Pintard et al., 2003 (link)). Western blots with anti–MEI-1 antibody indicated that this transgene is expressed at a much lower level than endogenous MEI-1 (not depicted), indicating that the localization in Fig. 5 is not due to overexpression. OD44, the GFP∷γ tubulin-expressing strain used in Fig. 7, was provided by A. Desai (University of California, San Diego, La Jolla, CA). mei-2(ct98) strains were maintained at 16°C and shifted to 20°C for 24 h before filming. EU1065 was maintained at 25°C to prevent germline silencing. All other strains were maintained at 20°C.
McNally K., Audhya A., Oegema K, & McNally F.J. (2006). Katanin controls mitotic and meiotic spindle length. The Journal of Cell Biology, 175(6), 881-891.
Publication 2006
Alleles Animals, Transgenic Antibodies, Anti-Idiotypic Caenorhabditis elegans Cloning Vectors Codon Germ Line Helminths Histone H2b Histones Homozygote Introns Mutation, Nonsense Strains Transgenes Tubulin Western Blot
2
Embryonic and Adult Worm Staining
Cited 8 times

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Publication 2009
Adult Agar Animals Antibodies Buffers Chromosome Segregation DAPI DNA, Helminth Embryo Fishes Fixatives Hermaphroditism Histone H2b Males Microscopy, Confocal Miotics Needles Syringes X Chromosome
3
Cloning and Transfection of VENUS Constructs
Cited 8 times
The N-terminal and C-terminal halves of VENUS (Nagai et al., 2002 (link)) were amplified by PCR and cloned into pCS2+. VENUS fragments were fused to the N-terminus of Human Smad4 with an 11 amino acids linker (TGGGGGGGGGG) or to the N-terminus of Xenopus Smad2 with a 4 amino acids linker (TGSS). For the constructs VC155-Smad3 and VC155-Smad2Δexon3, Smad2 was replaced either by Xenopus Smad3 (the gift of Caroline Hill) or Xenopus Smad2Δexon3 (the gift of Oliver Nentwich). Site-directed mutagenesis to create modified forms of VENUS was performed as described (Sawano and Miyawaki, 2000 (link)). VENUS, mRFP1, ECFP, ECFP-GPI, ECFP-NLS, Histone H2B-ECFP and Emerin-ECFP were all cloned into the expression vector pCS2+ and checked by sequencing. GFP-emerin was the kind gift of Chris Hutchison, YFP-histone H2B the kind gift of Andrea Brand, and the NLS sequence the kind gift of Steve Jackson.
All constructs in pCS2+ were linearised with NotI and transcribed in vitro using SP6 RNA polymerase and the mMESSAGE mMACHINE kit (Ambion). HEK293T cells were cultured in D-MEM medium (high glucose without sodium pyruvate; Gibco 41965-039) with 10% serum and transfected with 0.5-2 μg of each plasmid DNA using Lipofectamine 2000 (Invitrogen).
Saka Y., Hagemann A., Piepenburg O, & Smith J.C. (2007). Nuclear accumulation of Smad complexes occurs only after the midblastula transition in Xenopus. Development (Cambridge, England), 134(23), 4209-4218.
Publication 2007
Amino Acids Cells Cloning Vectors Culture Media emerin Glucose Histone H2b Homo sapiens lipofectamine 2000 Mutagenesis, Site-Directed Plasmids Pyruvate RNA polymerase SP6 Serum SMAD2 protein, human SMAD3 protein, human SMAD4 protein, human Sodium Xenopus laevis
4
Experimental Protocols for Visualizing Kinetochore Proteins
Cited 8 times
HeLa cells and clonal lines derived from HeLa cells stably expressing GFP-Mis12 (Cheeseman et al., 2004 (link)), YFP–CENP-A (a gift from D. Foltz, Ludwig Institute for Cancer Research [LICR], La Jolla, CA), and YFP-histone H2B (a gift from J. Shah, Harvard Medical School, Boston, MA; Kops et al., 2004 (link)), or GFP–CENP-H and GFP-hNuf2 (this study) were maintained in DME supplemented with 10% FBS, penicillin/streptomycin, and l-glutamine (Invitrogen) at 37°C in a humidified atmosphere with 5% CO2. Cells were plated on 12- or 22-mm glass coverslips coated with poly-l-lysine (Sigma-Aldrich) for immunostaining or 35-mm glass bottom microwell dishes (MatTek) for time-lapse imaging. Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410, hNnf1PMF1, hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; DeLuca et al., 2005 (link)), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen). FBS was added to 10% to the transfection reaction after incubation on cells for 5–6 h. Cells were assayed 48 h after transfection for hDsn1, hNnf1, or hNsl1 and 72 h after transfection for hMis12.
Chicken Mis12 target disruption constructs for each gene were generated such that genomic fragments encoding an entire coding region in a single exon were replaced with a histidinol or puromycin resistance cassette under control of the β-actin promoter. Target constructs were transfected with a Gene Pulser II electroporator (Bio-Rad Laboratories). Chicken DT40 cells were cultured and transfected as described previously (Fukagawa et al., 2001 (link), 2004 (link)). All DT40 cells were cultured at 38°C in DME supplemented with 10% FCS, 1% chicken serum, and penicillin/streptomycin. To repress the expression of the tet-responsive transgenes, tet (Sigma-Aldrich) was added to the culture medium to a final concentration of 2 μg/ml. Chicken homologues of Dsn1, Nsl1, and Nnf1 were cloned and fused with GFP under control of the cytomegalovirus promoter. These fusion constructs were transfected into chMis12 conditional knockout cells to examine their localization.
Kline S.L., Cheeseman I.M., Hori T., Fukagawa T, & Desai A. (2006). The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation. The Journal of Cell Biology, 173(1), 9-17.
Publication 2006
Actins Atmosphere Cells Chickens Culture Media Cytomegalovirus Exons Genes Genome Glutamine HeLa Cells Histidinol Histone H2b Hyperostosis, Diffuse Idiopathic Skeletal Lysine Malignant Neoplasms NUF2 protein, human oligofectamine Penicillins Poly A Puromycin RNA, Small Interfering Serum Streptomycin Transfection Transgenes
5
Fluorescent Cell Lines for Live-Cell Imaging
Cited 7 times
The following cell lines were used: MCF10A, MCF10A-CA1d (abbreviated as CA1d), SQ20B, and PC9. MCF10A and CA1d cells were cultured in DMEM/F12 medium containing 10% equine serum, 5% fetal bovine serum, 20 ng/ml epidermal growth factor, 10 μg/ml insulin, 500 ng/ml hydrocortisone, and 100 ng/ml cholera toxin. SQ20B cells were cultured in DMEM containing 20% fetal bovine serum and 400 ng/ml hydrocortisone, and PC9 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum. Primary human tumor-derived cells were obtained from a patient with squamous cell carcinoma of the tongue and grown continuously in culture in keratinocyte (serum-free) growth medium (Invitrogen). Cell lines were engineered to express the histone H2B/monomeric red fluorescent protein (H2B-mRFP) fusion protein using lentivirus-mediated transduction as previously described 31 (link). Brightly fluorescent single-cell clones were expanded and compared to parental populations using traditional proliferation measurements to ensure they were representative of the initial population. Primary cells were induced to express H2B-mRFP using recombinant baculoviral particles (CellLight® Nucleus, Invitrogen) at 20 particles per cell, according to the manufacturer’s instructions.
Tyson D.R., Garbett S.P., Frick P.L, & Quaranta V. (2012). Fractional Proliferation: A method to deconvolve cell population dynamics from single-cell data. Nature methods, 9(9), 923-928.
Publication 2012
Baculoviridae Cancer of Tongue Cell Lines Cell Nucleus Cells Cholera Toxin Clone Cells Culture Media Epidermal growth factor Equus caballus Fetal Bovine Serum Histone H2b Homo sapiens Hydrocortisone Insulin Keratinocyte Lentivirus Neoplasms Parent Patients red fluorescent protein Serum Squamous Epithelial Cells

Most recents protocols related to «Histone H2b»

1
Overexpression of FLAP-MASTL Resistant to Silencing

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Publication 2023
Cells Clone Cells Cloning Vectors DNA, Complementary Doxycycline Glycoproteins Histone H2b Infection Neomycin PAX2 protein, human Plasmids Polybrene Retroviridae RNA, Small Interfering Silent Mutation Surgical Flaps Vesicular stomatitis Indiana virus Virion
2
Visualizing Chromosome Segregation Dynamics

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Publication 2023
Catabolism Cells Chromatids Chromosome Segregation Division, Cell Histone H2b Microscopy Microscopy, Phase-Contrast Nuclear Envelope
3
Microscopic Imaging of Moss Chloronema
P. patens chloronema was observed using the MT marker (PpGCP4p::GFP–tubulin) (Kozgunova & Goshima, 2019 (link)), the dual-color marker of MT and nucleus (PpGCP4p::GFP–tubulin and 7113p::histone H2B–mRFP) (Kozgunova & Goshima, 2019 (link)), or Pp-Kinesin-12IIc and MT (Pp-Kinesin-12IIc–Citrine and PpACTp::mCherry–tubulin) (Miki et al, 2014 (link)).
For the PD-180970 experiment, chloronema tissues expressing MT marker cultured on a cellophane-laid BCDAT plate for 6 d were sonicated in a BCD liquid medium containing 10 μM oryzalin, 10 μM PD-180970, or 0.5% DMSO, followed by incubation for 30 min. The tissues were introduced into microfluidic devices and immediately observed (Kozgunova & Goshima, 2019 (link)). The images were acquired with an inverted microscope (Ti, 100 × 1.45 NA lens; Nikon) equipped with a spinning-disk confocal unit (CSU-X1; Yokogawa), 488- and 561-nm laser lines (LDSYS-488/561-50-YHQSP3, Pneum), and an electron-multiplying charge-coupled device camera (ImagEM; Hamamatsu) at 2.5-μm z-intervals. The microscope was controlled using NIS-Elements.
For PP2 experiments, chloronema tissues were cultured in six-well glass-bottom dishes or 35-mm dishes in a BCD agarose medium for 5–7 d (Yamada et al, 2016 (link)). Water containing 10 μM PP2 or 0.5% DMSO was directly applied to the dishes, and the mosses were incubated for 30 min before observation. High-resolution live-cell imaging of the Pp-Kinesin-12IIc/MT marker was performed using the same microscope described above. Long-term imaging of MT/histone markers was performed with a wide-field microscope (TE2000, 10 × 0.45 NA lens; Nikon) equipped with a CMOS camera (ZYLA-4.2P-USB3; Andor) and a Nikon Intensilight Epi-fluorescence illuminator, which was controlled by iQ software.
Kimata Y., Yamada M., Murata T., Kuwata K., Sato A., Suzuki T., Kurihara D., Hasebe M., Higashiyama T, & Ueda M. (2023). Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species. Life Science Alliance, 6(5), e202201657.
Publication 2023
Cell Nucleus Cellophane Cells Chronic multifocal osteomyelitis Electrons Fluorescence Histone H2b Histones Hyperostosis, Diffuse Idiopathic Skeletal Kinesin Lens, Crystalline Medical Devices Microchip Analytical Devices Microscopy Mosses oryzalin PD 180970 Pneumonia Sepharose Sulfoxide, Dimethyl Tissues Tubulin
4
Aging Study of Murine γδ T Cells
C57BL/6J and Tcrd−/− mice [9 (link)] were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Tcrd-H2BeGFP transgenic mice (C57BL/6 background) were a kind gift of Dr. Immo Prinz (Institute of Immunology, Hanover Medical School, Hanover, Germany). Tcrd-H2BeGFP mice are transgenic fluorescent reporter mice enabling the identification of γδ T lymphocytes due to an insertion of an expression cassette encoding an internal ribosomal entry site and a fusion of human histone H2B and green fluorescent protein (GFP) in the 3′ untranslated region of the Tcrd constant (C) gene [10 (link)]. Thus, GFP is expressed in nuclei of γδ T cells and labels only this cell population. All mice were housed at the Łukasiewicz Research Network–PORT Polish Center For Technology Development (Łukasiewicz-PORT) in Wroclaw, Poland, in individually ventilated cages in 12 : 12 h light-dark cycle under specific pathogen-free conditions with water and food available ad libitum. Young female mice at the age of 2 months were subcutaneously injected with 3 mg of medroxyprogesterone acetate (Depogeston, Biowet Puławy, Puławy, Poland) to induce diestrus. The stage of the estrous cycle was determined by vaginal smears. Females were kept at the Łukasiewicz-PORT's animal facility until they reached 18 months of age. All experiments were in accordance with the Local Ethics Committee for Experiments on Animals at Hirszfeld Institute of Immunology and Experimental Therapy in Wroclaw.
Skulska K., Kędzierska A.E., Krzyżowska M, & Chodaczek G. (2023). Age-Related Changes in Female Murine Reproductive Mucosa with respect to γδ T Cell Presence. Journal of Immunology Research, 2023, 3072573.
Publication 2023
Animals Cell Nucleus Cells Diestrus Estrous Cycle Females Food Genes Green Fluorescent Proteins Hartnup Disease Histone H2b Homo sapiens Internal Ribosome Entry Sites Medroxyprogesterone Acetate Mice, Laboratory Mice, Transgenic Regional Ethics Committees Specific Pathogen Free T-Cell Receptors delta-Chain T-Lymphocyte Therapies, Investigational Untranslated Regions Vaginal Smears
5
Cell Line Cultivation and Genetic Manipulation
The human BC cell lines, MDA-MB-231, MDA-MB-468, BT-549, SUM159, MCF-7 and HEK293T were obtained from ATCC. Wild-type and Cdh1−/− MEFs were provided by Dr. Wenyi Wei. Among them, MDA-MB-231, MCF-7, HEK293T, MDA-MB-468, BT-549 and MEF cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). SUM159 cells were cultured in RPMI-1640 medium supplemented with 10% FBS. MCF-10A cells were gifts from the S. D. Cappell group, and cultured in MEBM™ Basal Medium and Supplements (Lonza, CC-3150). All the cells used for the experiments were tested negative for mycoplasma contamination.
pLenti-HA-CDH1 was purchased from Applied Biological Materials Inc. pLKO-shRNF219, pLKO-shUBR5, pLKO-shRNF149, pLKO-shSMURF2, pLKO-shWWP2, pLKO-shUBE3A, pLKO-shUBE3B, pLKO-shNEDD4, pLKO-shKEAP1 and pLKO-shFBXO7 were purchased from Sigma-Aldrich. pLenti-HA-CDH1-6A, -7A, pLenti-3X Flag-PIN1, pLenti-3X Flag-PIN1-W34A, -W34A-RLAA, -RLAA, -M130L, -M130I, -C113A and -C113S were generated in our lab. mCherry-Geminin (1-110) and Histone H2B-Turquoise lentiviral vectors were provided by Dr. Jia-Yun Chen. His-ubiquitin constructs was provided by Dr. Yu-Ru Lee. The shRNA library for human E3 ubiquitin ligases (TRC library, RHS4896) was purchased from Thermo Scientific Open Biosystems.
Ke S., Dang F., Wang L., Chen J.Y., Naik M.T., Thavamani A., Liu Y., Li W., Kim N., Naik N.M., Sui H., Tang W., Qiu C., Koikawa K., Batalini F., Wang X., Clohessy J.G., Heng Y.J., Lahav G., Gray N.S., Zho X.Z., Wei W., Wulf G.M, & Lu K.P. (2023). Reciprocal inhibition of PIN1 and APC/CCDH1 controls timely G1/S transition and creates therapeutic vulnerability. Research Square.
Publication Preprint 2023
Biopharmaceuticals CDH1 protein, human cDNA Library Cell Lines Cells Cloning Vectors Culture Media Dietary Supplements Eagle Fetal Bovine Serum Gifts GMNN protein, human Histone H2b Homo sapiens Ligase, Ubiquitin-Protein Mycoplasma PIN1 protein, human Short Hairpin RNA Ubiquitin

Top products related to «Histone H2b»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Histone h2b by Cell Signaling Technology
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Histone H2B is a core histone protein that is a component of the nucleosome, the fundamental unit of chromatin. Histone H2B plays a critical role in the organization and compaction of DNA within the cell nucleus.
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Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Histone h2b by Abcam
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Histone H2B is a core histone protein that is a component of nucleosomes in eukaryotic cells. Histones play a fundamental role in the organization and compaction of DNA within the cell nucleus.
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Ab1790 by Abcam
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Ab1790 is a primary antibody that recognizes Actin, beta (ACTB) protein. It is suitable for use in various applications such as Western blotting and immunohistochemistry.
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Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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Anti histone h2b by Abcam
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Anti-Histone H2B is a laboratory reagent used in various research applications. It is a specific antibody that recognizes and binds to the histone H2B protein, which is a core component of nucleosomes in eukaryotic cells. The primary function of this product is to enable the detection and analysis of histone H2B in biological samples.
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Histone h3 by Cell Signaling Technology
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Histone H3 is a core histone protein that is a key component of the nucleosome, the basic structural unit of chromatin. Histones play a crucial role in the organization and regulation of eukaryotic DNA within the cell nucleus.
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β actin by Merck Group
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β-actin is a protein that is found in all eukaryotic cells and is involved in the structure and function of the cytoskeleton. It is a key component of the actin filaments that make up the cytoskeleton and plays a critical role in cell motility, cell division, and other cellular processes.
10
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

More about "Histone H2b"

Histone H2B (H2B) is a core histone protein that plays a crucial role in the organization and regulation of eukaryotic chromatin.
It is a key component of the nucleosome, the fundamental unit of chromatin, and is essential for maintaining chromatin structure and participating in various cellular processes, such as transcription, DNA repair, and epigenetic modifications.
H2B is closely related to other histone proteins, including Histone H3, and together they form the histone octamer that packages DNA into nucleosomes.
Understanding the functions and dynamics of H2B is crucial for advancing research in areas like gene expression, cellular differentiation, and chromatin-based diseases.
The PubCompare.ai platform can help streamline your H2B research by providing AI-driven comparisons of published literature, preprints, and patents.
This allows you to identify the most reproducible and accurate approaches for your experiments, such as using Histone H2B antibodies (Anti-Histone H2B) or transfecting cells with Histone H2B constructs using Lipofectamine 2000.
By leveraging the insights gained from H2B research, you can improve your experimental results and enhance your understanding of chromatin-related processes.
PubCompare.ai's powerful tools can help you navigate the wealth of information available, from Histone H2B detection and quantification to its role in gene regulation and cellular differentiation.
Whether you're working with H2B in the context of cell culture (using media like DMEM and supplements like FBS and Penicillin/streptomycin) or in animal models, PubCompare.ai can be a valuable resource to streamline your research and identify the most reliable and effective protocols and products, such as the Ab1790 antibody for Histone H2B detection.