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IGF1 protein, human

Insulin-like growth factor I (IGF1) is a polypeptide protein that plays a crucial role in human growth and development.
It is produced primarily by the liver and is essential for regulating cell proliferation, differentiation, and metabolism.
IGF1 exerts its effects through binding to specific cell surface receptors, triggering downstream signaling cascades that influence a variety of physiological processes.
Researchers studying IGF1 protein can utilize the PubCompare.ai platform to optimize their research, locating the best protocols and products from literature, pre-prints, and patents using AI-driven comparisons to ensure reproducible science.
This tool can help streamline IGF1 protein research effeciently, uncovering the power of this invaluable resource.

Most cited protocols related to «IGF1 protein, human»

1
Generation and Characterization of iPSCs
Cited 42 times

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Publication 2010
Biopsy Cloning Vectors DNA, Complementary Embryo Ethics Committees, Research Females Fibroblasts Gentamicin Homo sapiens Human Embryonic Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal IGF1 protein, human Induced Pluripotent Stem Cells Infection KLF4 protein, human Lentivirus matrigel MECP2 protein, human Mice, Laboratory Neurons Oncogenes, myc POU5F1 protein, human Retroviridae Short Hairpin RNA SOX2 protein, human Stem Cells Synapsin I Vertebral Column
2
Cohort Studies on Pubertal Height
Cited 24 times
Two data sets are used as examples, both of height in puberty. The first, the Christ’s Hospital School (CHS) cohort, consisted of a sample of 3245 boys, who attended the school at some stage between 1939 and 1968 and were measured twice a term between the ages of 9 and 19 years [median 42 measurements per boy, inter-quartile (IQR) range 35–47, range 1–63], a total of 129 508 heights. These were linked to follow-up data on 1520 adults, some 50 years later, when inter alia height and insulin-like growth factor 1 (IGF-1) levels were measured. The cohort has been described in detail previously,7–9 and an inverse association between age at peak height velocity (APHV) and later IGF-1 has been reported.9 (link) This was based on estimates of APHV for each child derived from spline curves fitted to each child’s data.
The second data set came from a randomized clinical trial of oxandrolone to increase final height in girls with Turner syndrome (TS). This is a chromosomal disorder where the second X chromosome is missing or malformed, leading to short stature and primary ovarian failure. A total of 106 girls with TS already on a standard dose of growth hormone were randomized to receive either oxandrolone or placebo from the age of 9 years (or the age at recruitment if later) until final height was reached. One girl dropped out immediately and 13 more later on, whereas 92 girls remained in the study and 82 had reached final height before the time of analysis. A total of 1321 heights were included in the analysis (median 12 per child, IQR 9–17, range 1–23). The trial, which also included a second randomization to early or late oestrogen for induction of puberty, is reported in detail elsewhere (EJ Gault et al., submitted for publication).
Cole T.J., Donaldson M.D, & Ben-Shlomo Y. (2010). SITAR—a useful instrument for growth curve analysis. International Journal of Epidemiology, 39(6), 1558-1566.
Publication 2010
Adult Child Disorder, Chromosomal Dwarfism Estrogens IGF1 protein, human Ovarian Failure, Premature Oxandrolone Placebos Puberty Turner Syndrome Woman X Chromosome Young Syndrome
3
Differentiation of Motor Neurons from hPSCs
Cited 19 times
To generate MNPs, hPSCs were dissociated with Dispase (1 mg/ml) and split 1:6 on irradiated MEFs or Matrigel coated plates. On the following day, the PSC medium was replaced with a chemically defined neural medium, including DMEM/F12, Neurobasal medium at 1:1, 0.5×N2, 0.5×B27, 0.1mM ascorbic acid (Santa Cruz), 1×Glutamax and 1×penicillin/streptomycin (All others from Invitrogen). CHIR99021 (3uM, Torcris), 2μM DMH-1 (Torcris) and 2μM SB431542 (Stemgent) were added in the medium. The culture medium was changed every other day. Human PSCs maintained under this condition for 6 days were induced into NEP cells. The NEP cells were then dissociated with Dispase (1 mg/ml) and split at 1:6 with the same medium described above. RA (0.1μM, Stemgent) and 0.5μM Purmorphamine (Stemgent) were added in combination with 1μM CHIR99021, 2μM DMH-1, and 2μM SB431542. The medium was changed every other day. NEP cells maintained under this condition for 6 days differentiated into OLIG2+ MNPs. The OLIG2+ MNPs were expanded with the same medium containing 3μM CHIR99021, 2μM DMH-1, 2μM SB431542, 0.1μM RA, 0.5μM Purmorphamine and 0.5 mM VPA (Stemgent), and split 1:6 once a week with Dispase (1 mg/ml). OLIG2+ MNPs were frozen with the regular frozen medium (DMEM/F12, 10% fetal bovine serum and 10% DMSO) in liquid nitrogen, and cultured again in expansion medium after thawing.
To induce MN differentiation, OLIG2+ MNPs were dissociated with Dispase (1 mg/ml) and cultured in suspension in the above neural medium with 0.5μM RA and 0.1μM Purmorphamine. The medium was changed every other day. OLIG2+ MNPs under this condition for 6 days differentiated into MNX1+ MNs. The MNX1+ MNs were then dissociated with Accumax (eBioscience) into single cells and plated on Matrigel coated plates or on astrocytes. The MNX1+ MNs were cultured with 0.5μM RA, 0.1μM Purmorphamine and 0.1μM Compound E (Calbiochem) for 10 days to mature into CHAT+ MNs. Insulin-like growth factor 1(IGF-1), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) (all from R&D, 10 ng/ml each) were added if MNs were plated at low density. For identifying MN disease phenotypes, SMA and ALS MNs were cultured without these neurotrophic factors.
Du Z.W., Chen H., Liu H., Lu J., Qian K., Huang C.T., Zhong X., Fan F, & Zhang S.C. (2015). Generation and Expansion of highly-pure Motor Neuron Progenitors from Human Pluripotent Stem Cells. Nature communications, 6, 6626.
Publication 2015
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide Ascorbic Acid Astrocytes Cells Chir 99021 Ciliary Neurotrophic Factor Cortisone dispase Fetal Bovine Serum Freezing Homo sapiens IGF1 protein, human matrigel Nerve Growth Factors Nervousness Neurotrophic Factor, Brain-Derived Nitrogen OLIG2 protein, human Pancreatic Stellate Cells Penicillins Phenotype purmorphamine Streptomycin Sulfoxide, Dimethyl
4
Quantitative Protein Profiling of IGF-1 Signaling
Cited 19 times
Serial diluted lysates were arrayed on nitrocellulose-coated FAST slides (Whatman, Kent, UK) using the Aushon 2470 Arrayer (Aushon Biosystems, Billerica, MA). Each slide was probed with a primary antibody plus a biotin-conjugated secondary antibody. The signal was amplified using the DakoCytomation-catalyzed system (Dako, Carpinteria, CA) and visualized using a 3,3′-diaminobenzidine colorimetric reaction. The slides were scanned, analyzed, and quantified using customized Microvigene software (VigeneTech, Inc., Carlisle, MA) to measure spot intensity. Each dilution curve was fitted with the logistic model “Supercurve Fitting” (39 ). The mean values of the protein levels in the nonstimulated cells were used to normalize the time courses of the phosphorylated proteins measured in IGF-1–stimulated cells.
Iadevaia S., Lu Y., Morales F.C., Mills G.B, & Ram P.T. (2010). Identification of optimal drug combinations targeting cellular networks: integrating phospho-proteomics and computational network analysis. Cancer research, 70(17), 6704-6714.
Publication 2010
Biotin Cells Colorimetry IGF1 protein, human Immunoglobulins Nitrocellulose Proteins Technique, Dilution
5
Differentiation of Motor Neurons from hPSCs
Cited 16 times
To generate MNPs, hPSCs were dissociated with Dispase (1 mg/ml) and split 1:6 on irradiated MEFs or Matrigel coated plates. On the following day, the PSC medium was replaced with a chemically defined neural medium, including DMEM/F12, Neurobasal medium at 1:1, 0.5×N2, 0.5×B27, 0.1mM ascorbic acid (Santa Cruz), 1×Glutamax and 1×penicillin/streptomycin (All others from Invitrogen). CHIR99021 (3uM, Torcris), 2μM DMH-1 (Torcris) and 2μM SB431542 (Stemgent) were added in the medium. The culture medium was changed every other day. Human PSCs maintained under this condition for 6 days were induced into NEP cells. The NEP cells were then dissociated with Dispase (1 mg/ml) and split at 1:6 with the same medium described above. RA (0.1μM, Stemgent) and 0.5μM Purmorphamine (Stemgent) were added in combination with 1μM CHIR99021, 2μM DMH-1, and 2μM SB431542. The medium was changed every other day. NEP cells maintained under this condition for 6 days differentiated into OLIG2+ MNPs. The OLIG2+ MNPs were expanded with the same medium containing 3μM CHIR99021, 2μM DMH-1, 2μM SB431542, 0.1μM RA, 0.5μM Purmorphamine and 0.5 mM VPA (Stemgent), and split 1:6 once a week with Dispase (1 mg/ml). OLIG2+ MNPs were frozen with the regular frozen medium (DMEM/F12, 10% fetal bovine serum and 10% DMSO) in liquid nitrogen, and cultured again in expansion medium after thawing.
To induce MN differentiation, OLIG2+ MNPs were dissociated with Dispase (1 mg/ml) and cultured in suspension in the above neural medium with 0.5μM RA and 0.1μM Purmorphamine. The medium was changed every other day. OLIG2+ MNPs under this condition for 6 days differentiated into MNX1+ MNs. The MNX1+ MNs were then dissociated with Accumax (eBioscience) into single cells and plated on Matrigel coated plates or on astrocytes. The MNX1+ MNs were cultured with 0.5μM RA, 0.1μM Purmorphamine and 0.1μM Compound E (Calbiochem) for 10 days to mature into CHAT+ MNs. Insulin-like growth factor 1(IGF-1), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) (all from R&D, 10 ng/ml each) were added if MNs were plated at low density. For identifying MN disease phenotypes, SMA and ALS MNs were cultured without these neurotrophic factors.
Du Z.W., Chen H., Liu H., Lu J., Qian K., Huang C.T., Zhong X., Fan F, & Zhang S.C. (2015). Generation and Expansion of highly-pure Motor Neuron Progenitors from Human Pluripotent Stem Cells. Nature communications, 6, 6626.
Publication 2015
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide Ascorbic Acid Astrocytes Cells Chir 99021 Ciliary Neurotrophic Factor Cortisone dispase Fetal Bovine Serum Freezing Homo sapiens IGF1 protein, human matrigel Nerve Growth Factors Nervousness Neurotrophic Factor, Brain-Derived Nitrogen OLIG2 protein, human Pancreatic Stellate Cells Penicillins Phenotype purmorphamine Streptomycin Sulfoxide, Dimethyl

Most recents protocols related to «IGF1 protein, human»

1
Cytokine and Angiogenic Factor Profiling

Protocol full text hidden due to copyright restrictions

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Publication 2023
angiogen Angiogenesis Factor Biological Assay Cytokine Enzyme-Linked Immunosorbent Assay Epidermal growth factor Fibroblast Growth Factor 2 Hypersensitivity IGF1 protein, human IL6 protein, human Immunoassay Insulin-Like Growth Factor I Leptin Proteins Transforming Growth Factor beta Tumor Necrosis Factor-alpha Vascular Endothelial Growth Factors
2
Evaluation of Growth Hormone Deficiency in Children
We selected 33 children with GHD diagnosed at Longgang District Maternity and Child Health Hospital as the disease group, and 24 healthy children of the same age as the control group. The ages of children in the two groups ranged between 5 and 14 years, with no statistical difference seen in the comparative analysis (P > 0.05) (Table 1). All the children with GHD were diagnosed at the Department of Growth and Development, Shenzhen Longgang District Maternity and Child Health Hospital. The disease group met the diagnostic criteria for GHD in Chinese children (15 ): ① Below the third percentile of the height of normal healthy children of the same age and sex (−1.88 standard deviations [−1.88 SD] or minus 2 standard deviations [−2 SD]); ② Annual growth rate <5 cm/year; ③ Symmetrical dwarfism and childish face; ④ Normal intelligence development; ⑤ Bone age lagging behind actual age; ⑥ Peak values of two GH drug provocation tests of <10 µg/L; ⑦ Lower than normal level of serum IGF-1. The exclusion criteria for children in the two groups included: ① Severe liver or gastrointestinal disorders; ② Severe infection; ③ Treatment with antibiotics or probiotic preparations within one month before the test. All children provided informed consent from their guardians before enrollment.
Huang C., Meng D., Li Y., Lu S., Yang W., Wu B., Chen S., Yang Z, & Liu H. (2023). Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency. Frontiers in Pediatrics, 11, 1133258.
Publication 2023
Antibiotics Bones Child Children's Health Chinese Diagnosis Dwarfism Face Gastrointestinal Diseases IGF1 protein, human Infection Legal Guardians Liver Probiotics Serum Substance Abuse Detection Vision
3
Microbiota Profiling and Function Analysis
The ade4 package in R (v3.3.3) software was used to perform principal component analysis (PCA) based on the composition and relative abundance of bacteria in all samples at the genus level. The overall distribution of the microbiota compositions in the two groups was plotted. Bacteria were classified to the phylum and genus levels, and different species between the two groups were investigated by the Wilcoxon method where P < 0.05 indicated a significant difference. The 16S rDNA sequencing data were used to evaluate differences in bacterial functions between the two groups of children based on the functional analysis performed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. SPSS 22.0 software was used for general data analysis. The age, weight, height, and IGF-1 values were compared by χ2 tests or two-group independent sample t-tests.
Huang C., Meng D., Li Y., Lu S., Yang W., Wu B., Chen S., Yang Z, & Liu H. (2023). Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency. Frontiers in Pediatrics, 11, 1133258.
Publication 2023
Bacteria Bacterial Physiological Phenomena Child DNA, Ribosomal Genome IGF1 protein, human Microbial Community
4
Pathway Analysis of Microglia and Astrocyte Subsets

Protocol full text hidden due to copyright restrictions

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Publication 2023
ADORA2B protein, human ADRB1 protein, human ApoE protein, human Astrocytes ATP2A2 protein, human ATP2B3 protein, human ATP5B protein, human ATP8A2 protein, human B3GNT5 protein, human c-Mer Tyrosine Kinase C3AR1 protein, human CAMKII gamma protein, human cardiotrophin-like cytokine Caspase 3 CCR5 protein, human CHRM3 protein, human CRKL protein CSF1 protein, human CTSB protein, human CTSD protein, human CTSL protein, human CTSS protein, human CUL1 protein, human CYBB protein, human DNM1L protein, human DRD1 protein, human EGR1 protein, human EMP1 protein, human ENO2 protein, human Esterase Inhibitor, C1 FRAP1 protein, human Galectin 3 Gene, c-fms Genes GPR56 protein, human GZMB protein, human IGF1 protein, human IGFBP5 protein, human IL1A protein, human IL1B protein, human MAFB protein, human Metabolism Microglia Nitric Oxide Synthase Type II OPA1 protein, human PARP2 protein, human PDPK1 protein, human phosphoglycerate mutase 1, human PPP3CB protein, human PPP3R1 protein, human PRF1 protein, human PRKACB protein, human PRKCI protein, human PSMB8 protein, human PTGS2 protein, human PTK2B protein, human PTPN1 protein, human Receptor, Transforming Growth Factor-beta Type I SMAD3 protein, human SPARC protein, human SPI1 protein, human SPP1 protein, human STAT1 protein, human TGFB1 protein, human TICAM1 protein, human Tissue Inhibitor of Metalloproteinase-2 TJP1 protein, human TLR2 protein, human TREM2 protein, human VDAC1 protein, human
5
Adipogenic Differentiation Assay
IGF-1 was purchased from Peprotech (Rocky Hill, NJ, USA). DMEM, Foetal bovine serum, β-Glycerol Phosphate, dexamethasone, ascorbic acid 2-phosphate, insulin, 3-Isobutyl-1-methylxanthine, indomethacin, rosiglitazone, Cetylpyridinium, Oil Red, Alizarin Red were purchased from Sigma-Aldrich (St. Louise, MO, USA). Other products were also purchased from Sigma-Aldrich unless otherwise indicated.
Gómez R., Barter M.J., Alonso-Pérez A., Skelton A.J., Proctor C., Herrero-Beaumont G, & Young D.A. (2023). DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation. Biological Research, 56, 9.
Publication 2023
1-Methyl-3-isobutylxanthine ascorbate-2-phosphate Cetylpyridinium Dexamethasone Fetal Bovine Serum Glycerophosphates IGF1 protein, human Indomethacin Insulin Rosiglitazone

Top products related to «IGF1 protein, human»

1
Igf 1 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China
The IGF-1 lab equipment is designed to measure and analyze insulin-like growth factor 1 levels in various samples. It is a versatile tool used in research and clinical settings to support studies related to growth, development, and metabolic processes.
2
Igf 1 by R&D Systems
Sourced in United States, United Kingdom, Germany, France
IGF-1 is a recombinant human insulin-like growth factor-1 protein. It is a member of the insulin-like growth factor family and plays a role in cell growth and differentiation.
3
Igf 1 by Merck Group
Sourced in United States, Germany, Switzerland, Italy, Japan, Spain, Sao Tome and Principe, United Kingdom
IGF-1 is a laboratory assay kit used to measure the levels of insulin-like growth factor 1 (IGF-1) in biological samples. IGF-1 is a hormone that plays a crucial role in growth and development processes.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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The Immulite 2000 is an automated immunoassay analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for hormones, proteins, and other analytes. The system utilizes chemiluminescent technology for detection and provides automated sample handling, reagent management, and result reporting.
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
10
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.

More about "IGF1 protein, human"

Insulin-like growth factor 1 (IGF-1), also known as somatomedin C, is a crucial polypeptide protein involved in human growth and development.
Produced primarily by the liver, IGF-1 plays a vital role in regulating cell proliferation, differentiation, and metabolism.
This potent growth factor exerts its effects by binding to specific cell surface receptors, triggering downstream signaling cascades that influence a variety of physiological processes.
Researchers studying IGF-1 can leverage the power of the PubCompare.ai platform to optimize their research efforts.
This AI-driven tool allows scientists to locate the best protocols and products from literature, pre-prints, and patents, ensuring reproducible and efficient IGF-1 protein research.
By utilizing the platform's AI-driven comparisons, researchers can uncover the most effective methods and materials, streamlining their investigations into this invaluable resource.
In addition to IGF-1, researchers may also work with other key substances, such as fetal bovine serum (FBS), which is commonly used as a cell culture supplement, and TRIzol reagent, a versatile solution for the isolation of RNA.
The Immulite 2000 analyzer can be employed for the quantitative determination of IGF-1 levels, while the RNeasy Mini Kit provides a reliable method for the purification of high-quality RNA.
Furthermore, antibiotics like penicillin and streptomycin are often used to prevent microbial contamination in cell culture experiments, and Dulbecco's Modified Eagle Medium (DMEM) is a widely used cell culture medium.
Lipofectamine 2000 is a transfection reagent that can be utilized for the efficient delivery of genetic material into cells, facilitating the study of IGF-1 signaling pathways and gene expression.
By incorporating these related terms and substances, researchers can gain a more comprehensive understanding of the broader context in which IGF-1 protein research is conducted, ultimately leading to more informed and impactful discoveries.