Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Amino Acid > IgG2B

IgG2B

IgG2B (Immunoglobulin G2b) is a subclass of immunoglobulin G, a type of antibody found in the blood and other bodily fluids.
IgG2B plays a crucial role in the adaptive immune response, providing protection against a variety of pathogens.
Researchers studying IgG2B can optimize their studies using PubCcompare.ai, an AI-powered platform that enhances reproducibility and accuracy.
PubCompare.ai helps researchers discover protocols from literature, preprints, and patents, and uses AI-driven comparisons to identify the best protocols and products for IgG2B research.
By improving research efficiency and success, PubCompare.ai empowers scientists to advance their understanding of this important immunoglobulin subclass.

Most cited protocols related to «IgG2B»

1
Preclinical Mouse Models for Immunotherapy
Cited 20 times
Five to seven week old female C57BL/6 and BALB/c mice were obtained from NCI Production (Frederick, MD) and Jackson Laboratory (Bar Harbor, ME) and maintained under pathogen free conditions. All animal experiments were performed according to protocols approved by the Institute of Animal Care and Use Committee of the University of Pennsylvania. For B16-F10 melanoma, 5×104 B16-F10 cells were mixed with an equal volume of Matrigel (BD Biosciences) and subcutaneously injected on the right flank of C57BL/6 mice on day 0 and the left flank on day 2. The right flank tumor site was irradiated with 20 Gy on day 8. Blocking antibodies were given on days 5, 8 and 11. For the concurrent vs. sequential RT experiment, the right flank was irradiated on either day 8 (sequential) or 12 (concurrent), while blocking antibodies were given on days 9, 12, and 15. For TSA breast cancer, 1×105 TSA cells were mixed with an equal volume of Matrigel (BD Biosciences) and subcutaneously injected on the right flank of BALB/c on day 0 and the left flank on day 2. The right flank of the mice was irradiated with 8 Gy on three consecutive days starting on day 10 or 11 post tumor implantation. Blocking antibodies were started 3 days prior to RT and given every 3 days for a total of 3 doses. For the pancreatic cancer model, 4×105 PDA.4662 cells were subcutaneously injected on the right flank. The right flank was irradiated with 20 Gy on day 8. Blocking antibodies were given on days 5, 8, and 11. For melanoma and breast cancer models, we used the optimal dose and fraction of radiation as previously reported23 (link),24 (link). All irradiation was performed using the Small Animal Radiation Research Platform (SARRP). Antibodies used for in vivo immune checkpoint blockade experiments were given intraperitoneally at a dose of 200 μg/mouse and include: CTLA4 (9H10), PD-1 (RMP1-14), PDL-1 (10F.9G2), CD8 (2.43), and rat IgG2B isotype (LTF-2) (BioXCell). Anti-CD8 was given 2 days prior to tumor implantations (day −2), day 0, then every 4 days for the duration of the experiment. Perpendicular tumor diameters were measured using calipers. Volume was calculated using the formula L × W2 × 0.52, where L is the longest dimension and W is the perpendicular dimension.
Victor C.T., Rech A.J., Maity A., Rengan R., Pauken K.E., Stelekati E., Benci J.L., Xu B., Dada H., Odorizzi P.M., Herati R.S., Mansfield K.D., Patsch D., Amaravadi R.K., Schuchter L.M., Ishwaran H., Mick R., Pryma D.A., Xu X., Feldman M.D., Gangadhar T.C., Hahn S.M., Wherry E.J., Vonderheide R.H, & Minn A.J. (2015). Radiation and Dual Checkpoint Blockade Activates Non-Redundant Immune Mechanisms in Cancer. Nature, 520(7547), 373-377.
Publication 2015
Animals Antibodies Antibodies, Blocking Cells CTLA4 protein, human IgG2B Immune Checkpoint Blockade Immunoglobulin Isotypes Malignant Neoplasm of Breast matrigel Melanoma Melanoma, B16 Mice, Inbred BALB C Mice, Inbred C57BL Mus Neoplasms Ovum Implantation Pancreatic Carcinoma pathogenesis Radiation Radiotherapy Woman
2
Propagation of Dengue Virus Strain S221
Cited 19 times
The hybridoma clones SFR3 and 2.43, which produce rat anti-human HLA DR5 and anti-mouse CD8 IgG2b Ab, respectively, were from the American Type Culture Collection and were grown in protein-free hybridoma medium supplemented with penicillin, streptomycin, HEPES, GlutaMAX, and 2-ME (all from Invitrogen) at 37°C/5% CO2. C6/36, an Aedes albopictus cell line, was cultured in Leibovitz's L-15 medium (Invitrogen) supplemented with 10% FBS (Gemini Bio-Products), penicillin, streptomycin, and HEPES at 28°C in the absence of CO2. Alternate passaging of PL046 (a DENV2 clinical isolate obtained from Dr. H.-Y. Lei (National Cheng Kung University, Taiwan) (20 (link))) through the sera of IFN-α/βR-/-, IFN-γR-/- mice, and C6/36 cells 10 times resulted in a novel DENV2 strain, termed D2S10, that causes more severe disease in those mice (19 (link)). S221, which differs from PL046 at E124, E128, and NS1228, is a triple-plaque-purified clone from the D2S10 population. Baby hamster kidney cells (BHK-21) were used to perform standard plaque assays, as described previously (21 (link)). Plaque-purified virus was amplified by two passages through C6/36 cells as described previously (21 (link)). Briefly, C6/36 monolayers were infected with DENV and cultured for 7 days. Viral supernatant was concentrated by ultracentrifugation at 50,000 × g for 90 min, resuspended in 20% FBS/PBS, and stored at -80°C. Infectious doses were determined based on genomic equivalents (GE), which were quantified by real-time RT-PCR. There are ~104 GE/PFU for S221.
Yauch L.E., Zellweger R.M., Kotturi M.F., Qutubuddin A., Sidney J., Peters B., Prestwood T.R., Sette A, & Shresta S. (2009). A Protective Role for Dengue Virus-Specific CD8+ T Cells. Journal of immunology (Baltimore, Md. : 1950), 182(8), 4865-4873.
Publication 2009
Aedes Biological Assay Cell Lines Cells Clone Cells Culture Media Dental Plaque Genome Hamsters HEPES HLA-DR5 Antigen Homo sapiens Hybridomas IgG2B Infant Infection Interferon-alpha Kidney Mus Penicillins Proteins Real-Time Polymerase Chain Reaction Serum Strains Streptomycin Ultracentrifugation Virus
3
Nanoscale Flow Cytometry for EV Analysis
Cited 15 times
The laboratory-built nFCM reported before was used in the present study, and Figure 1(a) shows the schematic diagram of the instrument design [43 (link),47 (link)]. Briefly, two single-photon counting avalanche photodiodes (APDs) were used for the simultaneous detection of the side scatter (SSC) (bandpass filter: FF01 − 524/24) and orange fluorescence (bandpass filter: FF01 − 579/34) of individual EVs, respectively. Unless stated otherwise, each distribution histogram or dot-plot was derived from data collected 1 min unless stated otherwise. Ultrapure water supplied by an ultrapure water system (PURELAB Ultra FLC00006307, ELGA) served as the sheath fluid via gravity feed.
For nFCM analysis, the sample stream is completely illuminated within the central region of the focused laser beam, and the detection efficiency is approximately 100%, which leads to accurate particle concentration measurement via single particle enumeration [49 (link),50 (link)]. The concentration of each EV sample was determined by employing 100 nm orange FluoSpheres of known particle concentration to calibrate the sample flow rate. Several dilutions were made to the orange FluoSpheres solution, and a linear correlation between particle concentration and detected event rate was obtained with R2 of 0.999 (data not shown). Regarding immunofluorescent staining, the following antibodies were purchased from BD Biosciences: PE-conjugated mouse anti-human CD9 antibody (clone M-L13), PE-conjugated mouse anti-human CD63 antibody (clone H5C6), PE-conjugated mouse anti-human CD81 antibody (clone JS-81), PE-conjugated mouse anti-human CD235a (clone GA-R2), PE-conjugated mouse anti-human CD45 (clone HI30), PE-conjugated mouse anti-human CD41a (clone HIP8), PE-conjugated mouse anti-human CD144 (clone 55-7H1), PE-conjugated mouse IgG1, κ (clone MOCP-21), and PE-conjugated mouse IgG2b, κ (clone 27–35). For phenotyping of EVs derived by HCT15 cells, EVs were isolated from 1 mL CCCM diluted to 12.5 mL with PBS by centrifugation once at 100,000 × g for 2 h at 4°C (Beckman Coulter X-90 centrifuge, SW 41 Ti rotor), and the pellet was resuspended in 50 μL PBS. EV preparation from PFP by UC or commercial kit was divided into 50 μL with a particle concentration of 6 × 108 particles/mL. Into each 50 μL EV sample, 20 μL of PE-conjugated antibody against CD9, CD63, CD81, CD235a, CD45, CD41a, CD144, IgG1 or IgG2b was added. The mixture was incubated at 37°C for 30 min and then washed twice with 1 mL PBS by UC at 100,000 × g for 17 min at 4°C (Beckman Coulter MAX-XP centrifuge, TLA-120.2 rotor). The pellet was resuspended in 50 μL PBS for nFCM analysis. For fluorescent labelling of phosphatidylserine (PS), all the other experimental procedures were the same as immunofluorescent staining except for replacing 20 μL PE-conjugated antibody with 5 μL PE-conjugated Annexin V (BD Bioscience). Meanwhile, the PBS buffer was replaced with 1 × annexin-binding buffer (Invitrogen, V13241) starting from EV preparation.
Tian Y., Gong M., Hu Y., Liu H., Zhang W., Zhang M., Hu X., Aubert D., Zhu S., Wu L, & Yan X. (2019). Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry. Journal of Extracellular Vesicles, 9(1), 1697028.
Publication 2019
Annexin A5 Annexins Antibodies Antibodies, Anti-Idiotypic Avalanches Buffers Cells Centrifugation Clone Cells Fluorescence Fluorescent Antibody Technique Glycophorin A Gravity Homo sapiens IgG1 IgG2B Immunoglobulins Immunoglobulin Variable Region Mus Phosphatidylserines Technique, Dilution
4
Mitochondrial ROS Quantification in Macrophages
Cited 11 times
Since MitoTracker Red and MitoSOX have very similar excitation and emission wavelengths, these two probes cannot be used together. Therefore, we stained MitoSOX separately, using the panel from Table 2.

Flow cytometry panel for evaluation of mitochondrial ROS in BMDMs.

Table 2
Target/ProbeFluorochromeClone/ Cat. number
Live/Dead CalceinBlue Excitation⁄Emission (nm): 495/515BD Calcein Blue AM Fluorescent Dye FisherScientific Catalog number: BDB564060
Anti-mouse CD11bPE-Cy7 Excitation⁄Emission (nm): 496/785Clone: M1/70 Isotype: Rat IgG2b, κ Biolegend Catalog number: 101,216
MitoSOX™PE - Red Excitation⁄Emission (nm): 510/580MitoSOX™ Red Mitochondrial Superoxide Indicator Invitrogen™ Catalog number: M36008
For macrophage surface staining and Live/ Dead staining, we diluted the anti-CD11b antibody 1/200 and the Live/Dead probe was diluted 1/500 in PBS + 2% FCS.
From the stock solution of MitoSOX Red, we suggest diluting 1/10 in PBS and then 1/100 into the final mix. This results in a final working concentration of 1/1000, or 5 µM.
Again, we determine one well for our unstained control and 1 well for MitoSOX Red FMO. The FMO consists of 50 µL of all dyes, except MitoSOX Red.
Pre-warm the mix (37 °C) and stain the cells as described above in the cell staining guide.
Monteiro L.D., Davanzo G.G., de Aguiar C.F, & Moraes-Vieira P.M. (2020). Using flow cytometry for mitochondrial assays. MethodsX, 7, 100938.
Full text: Click here
Publication 2020
Antibodies, Anti-Idiotypic Cells dye 50 Fluorescent Dyes fluorexon IgG2B Immunoglobulin Isotypes ITGAM protein, human Macrophage Mitochondrial Inheritance MitoSOX Mus Stains Superoxides
5
Detailed Antibody Characterization in Alzheimer's
Cited 11 times
For the methods used in this study, the following primary antibodies were utilized: MOAB-2 (anti-Aβ, mouse IgG2b, 0.5 mg/ml), IgG2b (0.2 mg/ml, Sigma-Aldrich, St. Louis, MO), 6E10 anti-Aβ residues 3-8, mouse IgG1, 0.5 mg/ml; Covance, Princeton, NJ), 22C11 (anti-APP N-terminal, mouse IgG1, 1 mg/ml, Milipore, Billerica, MA), 4G8 anti-Aβ residues 17-24, mouse IgG, Senetek, Maryland Height, MD), CT1565 (anti-APP C-terminal, rabbit monoclonal #1565, 0.45 mg/ml, Epitomics, Burlingame, CA), CT695 (anti-APP C-terminal, rabbit polyclonal #51-2700, 0.25 mg/ml, Invitrogen, Carlsbad, CA), anti-Aβ40 (MM32-13.1.1, mouse IgG1, 1.8 mg/ml, Bu lab), anti-Aβ42 (rabbit, 0.35 mg/ml; Invitrogen, Carlsbad, CA), anti-β-actin (chicken, 1.0 mg/ml; Abcam, Cambridge MA) cathepsin-D (goat polyclonal, 0.2 mg/ml, Santa Cruz biotechnology, Santa Cruz, CA). The dilutions of each antibody stock are denoted in the appropriate Methods section or Figure Legend.
Youmans K.L., Tai L.M., Kanekiyo T., Stine WB J.r., Michon S.C., Nwabuisi-Heath E., Manelli A.M., Fu Y., Riordan S., Eimer W.A., Binder L., Bu G., Yu C., Hartley D.M, & LaDu M.J. (2012). Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody. Molecular Neurodegeneration, 7, 8.
Full text: Click here
Publication 2012
Actins Antibodies Cathepsin D Chickens Goat IgG1 IgG2B Immunoglobulins Mice, House Rabbits Technique, Dilution

Most recents protocols related to «IgG2B»

1
Enhancing Antibody Responses with Strategic T-Cell Epitope Placement
Not available on PMC !

Example 3

Serum was obtained from mice immunized with the composite influenza peptides Pep 63 and Pep 64 both in conjugated and unconjugated forms. These serum sample were tested for IgG1, IgG2a and IgG2b activity against Pep 3, Pep 6, Pep 10, and Pep 11 (Pep 11—the composite 3, 6 and 10 peptides).

With regard to Pep 3, Pep 6, Pep 10, and Pep 64, both conjugated and unconjugated, as compared to Pep 63 showed an overall greater IgG1 response (FIGS. 2, 3 and 4). With regard to Pep 11, Pep 64, both conjugated and unconjugated, as compared to Pep 63 also showed a greater IgG1 response (FIG. 5).

With regard to Pep 3, Pep 6, and Pep 10, there was a minimal IgG2a response to either Pep 63 or Pep 64, whether in conjugated or unconjugated form (FIGS. 6-8). With regard to Pep 11, Pep 64, conjugated and unconjugated showed only a weak IgG2a response; conjugated greater than unconjugated (FIG. 9).

With regard to Pep 3, Pep 6, Pep 10 there was a greater IgG2b response to Pep 64, conjugated, as compared to Pep 63 which mostly appeared after booster was administered (FIGS. 10-12). With regard to Pep 11, Pep 64, conjugated, showed a very large IgG2b response that was enhanced after the booster was administered (FIG. 13).

Pep 64 (both conjugated and unconjugated) with the T-cell epitope at the N-terminal end induced increased serum antibody responses to the individual peptides across IgG1 and IgG2b isotypes, but not IgG2a. What this data clearly shows is that the location of the T cell epitope on an antigen can have a significant effect of how the antigen is seen and responded to by the host immune system. These data also indicate that T cell epitope placement can have a profound effect on both the Th-1 and Th-2 responses.

US11866463B2. Immunogenic compositions to treat and prevent microbial infections (2024-01-09). Longhorn Vaccines and Diagnostics, LLC [US]. Inventors: Luke T. Daum [US], Gerald W. Fischer [US], Clara J. Sei [US].
Full text: Click here
Patent 2024
Antibody Formation Antigens cyclo-acetyl-(cysteinyl-histidyl-phenylalanyl-glutaminyl-phenylalanyl-cysteinyl)amide Debility Epitopes, T-Lymphocyte IgG1 IgG2A IgG2B Immunoglobulin Isotypes Mus Peptides Secondary Immunization Serum System, Immune Virus Vaccine, Influenza Vision
2
Immunofluorescence Analysis of Muscle Tissue
The muscles were cut on a cryostat at − 23 °C (7 μm), air-dried, and stored at − 20 °C. Slides were air-dried, rehydrated, and fixed in 4% paraformaldehyde (PFA) for 20 min at the time of staining. For CD63/DAPI/laminin staining, sections were incubated with mouse anti-CD63 IgG1 antibody (1:100 dilution, ab108950, Abcam, Cambridge, UK) and rabbit anti-laminin IgG antibody (1:100 dilution, L9393, Sigma-Aldrich, St. Louis, MO) overnight at 4 °C. Slides were washed in PBS, then incubated with Alexa Fluor 488 goat anti-mouse IgG1 (1:250 dilution, A11001, Invitrogen, Waltham, MA) and Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) secondary antibodies for 1 h at room temperature. Slides were washed in PBS and mounted with VectaShield fluorescent mounting media with DAPI (H-1200-10, Vector Laboratories, Newark, CA). For CD9/DAPI/dystrophin staining, sections were incubated with rabbit anti-CD9 IgG (1:100 dilution, SA35-08, Invitrogen) and mouse anti-dystrophin IgG2b (1:250 dilution, 08168, Sigma-Aldrich) overnight, followed by incubation with Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) and Alexa Fluor 647 goat anti-mouse IgG2b (1:250 dilution, A32728, Invitrogen) for 1 h at room temperature. For CD81/DAPI/dystrophin staining, sections were incubated with rabbit anti-CD81(1:100 dilution, SN206-01, Novus Biologicals, Centennial, CO) and mouse anti-dystrophin IgG2b (1:250 dilution, 08168, Sigma-Aldrich) overnight, followed by incubation with Alexa Fluor 594 goat anti-rabbit IgG (1:250 dilution, A11012, Invitrogen) and Alexa Fluor 647 goat anti-mouse IgG2b (1:250 dilution, A32728, Invitrogen) for 1 h at room temperature. For Pax7/CD9/DAPI/WGA staining, sections were subjected to epitope retrieval using sodium citrate (10 mM, pH 6.5) at 92 °C, followed by blocking of endogenous peroxidase activity with 3% hydrogen peroxide in PBS. Sections were incubated overnight in mouse anti-Pax7 IgG1 (1:100 dilution, Developmental Studies Hybridoma Bank, Iowa City, IA) and rabbit anti-CD9 IgG (1:100 dilution, SA35-08, Invitrogen), followed by incubation in goat anti-mouse biotin-conjugated secondary antibody (dilution 1:1,000, 115-065-205; Jackson ImmunoResearch, West Grove, PA) and Alexa Fluor 647 goat anti-rabbit IgG (1:250 dilution, A32733, Invitrogen) for 1 h at room temperature. Next, sections were incubated with streptavidin-HRP (1:500 dilution, S-911, Invitrogen) and Texas Red-conjugated Wheat Germ Agglutinin (WGA) (1:50 dilution, W21405, Invitrogen) at room temperature for 1 h, before incubation in Tyramide Signal Amplification (TSA) Alexa Fluor 488 (1:500 dilution, B40953, Invitrogen). Sections were mounted with VectaShield fluorescent mounting media with DAPI (H-1200-10, Vector Laboratories).
Images were captured with a Zeiss upright microscope (AxioImager M1, Oberkochen, Germany). To quantify the percentage of nuclei (DAPI+) expressing CD63, MyoVision software was used for automated analysis of nuclear density in cross-sections [39 (link)], and nuclei-expressing CD63 (identified as DAPI+/CD63+ events) were counted manually in a blinded manner by the same assessor for all sections using the Zen Blue software.
Ismaeel A., Van Pelt D.W., Hettinger Z.R., Fu X., Richards C.I., Butterfield T.A., Petrocelli J.J., Vechetti I.J., Confides A.L., Drummond M.J, & Dupont-Versteegden E.E. (2023). Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy. Skeletal Muscle, 13, 6.
Full text: Click here
Publication 2023
Alexa594 alexa fluor 488 Alexa Fluor 647 anti-IgG Antibodies Antibodies, Anti-Idiotypic Biological Factors Biotin Cardiac Arrest Cell Nucleus Cloning Vectors DAPI DMD protein, human Epitopes Goat Hybridomas IgG1 IgG2B Immunoglobulins Laminin Microscopy Mus Muscle Tissue Novus paraform PAX7 protein, human Peroxidase Peroxides Rabbits Sodium Citrate Streptavidin Technique, Dilution Tritium Wheat Germ Agglutinins
3
Murine Myeloid Cell Isolation Protocol
Estradiol benzoate (HY-B1192) was purchased from MCE. Gox (G7141), collagenase type IV (C5138) and DNase I (11284932001) were purchased from Sigma-Aldrich. Fluorochrome-labeled or unlabeled monoclonal antibodies against mouse Ly6G (108454, 127607), IgG2b isotype control (400675), CD16/32 (101319), CD45 (103113), CD11b (101227), F4/80 (123115), FcγR III (158013) were purchased from BioLegend. Recombinant anti-myeloperoxidase antibody (ab208670) was purchased from Abcam. DMEM/F-12 GlutaMAX (SH30023.01) was purchased from Hyclone. 2-NBDG (N13195) was purchased from Invitrogen. Cell Counting Kit-8 (E-CK-A362) was purchased from Elabscience. BSA-FITC, BSA-ICG were purchased from Xi’an ruixi Biological Technology Co., Ltd.
Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.
Full text: Click here
Publication 2023
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose Antibodies, Anti-Idiotypic Biopharmaceuticals Collagenase Deoxyribonuclease I estradiol 3-benzoate Fluorescein-5-isothiocyanate Fluorescent Dyes IgG2B Immunoglobulin Isotypes ITGAM protein, human Monoclonal Antibodies Mus Peroxidase
4
Neutrophil Depletion for BSA-NP Study
To deplete neutrophils, mice were treated with a 400 μg intraperitoneal injection of anti-mouse Ly6G antibody (108454, Biolegend) or IgG2b isotype control (400675, Biolegend) 24 h prior to injection of BSA-NPs. Subsequent 200 μg antibody injections were administered accompanied by BSA-NPs. Animals were euthanized after 16 h to collect peritoneal lavage fluid, ectopic lesions, and eutopic endometrium. Neutrophil depletion was confirmed by flow cytometry as described below.
Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.
Full text: Click here
Publication 2023
Animals Endometrium Flow Cytometry IgG2B Immunoglobulin Isotypes Mus Neutrophil Passive Immunization Peritoneal Fluid Peritoneal Lavage
5
Biodistribution of Nanomaterials in Endometriosis
30 μL 10 mg/ml FITC-BSA-NPs, ICG-BSA-NPs, FITC-BSA-GOx-NPs or ICG-BSA-GOx-NPs were i.p. or i.v. injected into endometriosis mice. After 16 h without further statement, the major organs of mice, including heart, liver, spleen, lung, kidney, eutopic endometrium and ectopic lesions, were collected and fluorescent images of these organs were acquired with IVIS. Moreover, 400 μg anti-Ly6G antibody or an IgG2b isotype control antibody was administered intraperitoneally 24 h prior to injection of FITC-BSA-NPs. Subsequent 200 μg antibody injections were intraperitoneal administered accompanied with FITC- BSA-NPs. Animals were euthanized after 16 h to collect the major organs and fluorescent images were acquired.
Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.
Full text: Click here
Publication 2023
Animals Antibodies, Anti-Idiotypic Endometriosis Endometrium fluorescein isothiocyanate bovine serum albumin Heart IgG2B Immunoglobulin Isotypes Immunoglobulins Kidney Liver Lung Mus Passive Immunization Spleen

Top products related to «IgG2B»

1
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
2
Igg2b by Southern Biotech
Sourced in United States
IgG2b is a type of immunoglobulin G (IgG) subclass that is commonly used in laboratory research and applications. It functions as an antibody, playing a role in the immune system's response to foreign substances. IgG2b has specific structural and functional characteristics that make it a valuable tool for various experimental and analytical purposes.
3
Facscanto 2 by BD
Sourced in United States, Germany, United Kingdom, Belgium, China, Australia, France, Japan, Italy, Spain, Switzerland, Canada, Uruguay, Netherlands, Czechia, Jersey, Brazil, Denmark, Singapore, Austria, India, Panama
The FACSCanto II is a flow cytometer instrument designed for multi-parameter analysis of single cells. It features a solid-state diode laser and up to four fluorescence detectors for simultaneous measurement of multiple cellular parameters.
4
Facscalibur flow cytometer by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Belgium, France, Spain, Italy, Australia, Finland, Poland, Switzerland, Cameroon, Uruguay, Denmark, Jersey, Moldova, Republic of, Singapore, India, Brazil
The FACSCalibur flow cytometer is a compact and versatile instrument designed for multiparameter analysis of cells and particles. It employs laser-based technology to rapidly measure and analyze the physical and fluorescent characteristics of cells or other particles as they flow in a fluid stream. The FACSCalibur can detect and quantify a wide range of cellular properties, making it a valuable tool for various applications in biology, immunology, and clinical research.
5
Facsdiva software by BD
Sourced in United States, Germany, United Kingdom, France, Canada, Belgium, Australia, Italy, Spain, Switzerland, China, Netherlands, Finland, Japan, Jersey, Lao People's Democratic Republic
FACSDiva software is a user-friendly flow cytometry analysis and data management platform. It provides intuitive tools for data acquisition, analysis, and reporting. The software enables researchers to efficiently process and interpret flow cytometry data.
6
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
7
Igg2c by Southern Biotech
Sourced in United States
IgG2c is a type of immunoglobulin G (IgG) antibody. It is a laboratory product that can be used for various research and analytical applications involving the detection and quantification of IgG2c in biological samples.
8
Rat igg2b by BioXCell
Sourced in United States
Rat IgG2b is an immunoglobulin subclass produced by rats. It is a laboratory reagent used for various research applications.
9
Igg2a by Southern Biotech
Sourced in United States
IgG2a is an immunoglobulin subclass that functions as an antibody. It is produced by plasma B cells and plays a role in the adaptive immune response.
10
Be0090 by BioXCell
Sourced in United States
BE0090 is a laboratory equipment designed for various scientific applications. It serves as a device for performing tasks such as mixing, stirring, or agitating samples. The core function of this product is to provide controlled and consistent movement or agitation of liquids or other materials within a laboratory setting.

More about "IgG2B"

Immunoglobulin G2b, IgG, adaptive immune response, pathogens, PubCompare.ai, AI-powered platform, reproducibility, accuracy, protocols, literature, preprints, patents, AI-driven comparisons, FACSCalibur, FACSCanto II, FACSDiva software, Bovine serum albumin, IgG2b, Rat IgG2b, IgG2a, BE0090