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Insulin pork

Insulin pork refers to the use of pork-derived insulin for the treatment of diabetes mellitus.
Pork insulin is structurally similar to human insulin and has been used as an effective alternative to bovine insulin.
This page provides an overview of research on insulin pork, including optimization of protocols, locating best procedures from literature and patents, and data-driven comparisons to improve reproducibility and accuracy.
Discover how PubCompare.ai can enhance your insulin pork research and experiance the power of data-driven comparisons to advance your work in this area.

Most cited protocols related to «Insulin pork»

Type 2 diabetic rats were induced by the combination of a high-fat diet (HFD) and a low-dose STZ as described by Srinivasan
[16 (link)]. Male Sprague-Dawleys (SD) rats weighing 200–220 g were obtained from the Experimental Animal Center of the Hebei Medical University, Shijiazhuang, Hebei Province, China. After 1 week of acclimation, the rats were randomly divided into a control group and a challenge group, and they were fed a normal pellet diet (NPD) consisting of 10.3% fat, 24.2% protein and 65.5% carbohydrate and a HFD consisting of 59.8% fat (mainly pork), 20.1% protein and 20.1% carbohydrate. After 8 weeks of dietary manipulation, an oral glucose tolerance test (OGTT) was performed as per the literature method to confirm the insulin resistance (IR) of the challenge group rats
[17 (link)].
Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ; Alexis, USA; 25 mg/kg i.p. in a 0.1 mol/L citrate buffer, pH 4.5) given to rats with insulin resistance. The NPD rats (CON group) were given via the i.p. route in a citrate buffer vehicle. One week after STZ administration, rats with fasting blood glucose (FBG) ≥11.1 mmol/L in two consecutive analyses were considered to be the type 2 diabetic rat model (DM group). All animals remained on the assigned diet until the terminal experiment.
After 8 weeks of diabetes, the FBG was performed again: CON (5.0 ± 0.4 mmol/L) and DM (12.7 ± 1.9 mmol/L). Then, the rats were anaesthetized by using 20% urethane (1.2 g/kg intraperitoneally), and plasma (8–10 ml per animal) was immediately collected from the femoral artery and processed into serum. After being washed in ice-cold saline solution, the hearts were weighed and frozen in liquid nitrogen, then stored at −80°C. All experimental procedures were performed in accordance with the guidelines established by the Ethics Review Committee for Animal Experimentation (Hebei Medical University, Shijiazhuang, China).
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Publication 2012
Acclimatization Animals Blood Glucose Buffers Carbohydrates Citrates Common Cold Diabetes Mellitus Diet Diet, High-Fat Femoral Artery Freezing Heart Injections, Intraperitoneal Insulin Resistance Males Nitrogen Oral Glucose Tolerance Test Plasma Pork Proteins Rats, Sprague-Dawley Rattus norvegicus Saline Solution Serum Therapy, Diet Urethane
The first animal experiment was performed using 45 lean male mice, 8 weeks old. After one week of acclimatization, body mass composition was determined and mice were divided into groups with similar body weight (25 ± 0.7 g), fat mass (2.5 ± 0.2 g) and lean mass (18.5 ± 0.6 g). The mice were fed the experimental diets (Table 1) ad libitum, including a LF diet, a high-fat/high-sucrose (HF/HS) diet and HF/HP diets based on either casein, cod or pork for 12 weeks (n = 9). All animals were subjected to an oral glucose tolerance test (OGTT) and an insulin tolerance test (ITT) after 10 and 11 weeks, respectively, as further described in Section 2.5. In week 6, total feces was collected during 7 days from all the individual cages, weighed and analyzed for total nitrogen and total fat content as previously described [29 (link)]. Apparent digestibility of nitrogen and fat were determined using the total fat and nitrogen content in the collected feces, in addition to the total dietary fat and nitrogen intake during the 7 days of feces collection.
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Publication 2019
Acclimatization Animals Body Composition Body Weight Caseins Diet Diet, High-Fat Dietary Fats Feces Immune Tolerance Insulin Males Mice, House Nitrogen Oral Glucose Tolerance Test Pork Sucrose Therapy, Diet
The control group (CON, n=8) was fed a standard ad libitum diet, consisting of dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]).
For the high-fat diet groups, food was presented from 9:00am to 10:00am each day. Animals were fed a daily diet of one can of Hill’s Prescription Diet (415g; 10% carbohydrate, 9% protein 8% fat, 0.3% fiber and 73% moisture [Hill’s Pet Nutrition, Topeka KS, USA]) and 825g dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]), supplemented with either 6g/kg of rendered pork fat (LARD, n=8) or salmon oil (SO, n=8). These alternative diets contained identical macronutrient content consisting of 21,025kJ/day comprised of 27% carbohydrate, 19% protein and 53% fat. The added lard consisted of 38.5% saturated fatty acid, 10.8% polyunsaturated fatty acid (10% ω-6; 0.8% ω-3), 44.6% monounsaturated fatty acid, and 0.1% cholesterol. The Salmon oil addition consists of 19.9% saturated fatty acid, 40.4% polyunsaturated fatty acid (1.5% ω-6; 35.5% ω-3).
The acute effects of six weeks of LARD and SO diets on insulin sensitivity and cardiac function have been reported previously (15 ). The present study reports data on extended diet durations of 3.0±0.3 months of lard feeding and 3.9±0.1 months of salmon oil feeding.
Publication 2017
Animals Canis familiaris Carbohydrates Cholesterol Diet Diet, High-Fat Fatty Acids, Monounsaturated Fibrosis Food Heart Insulin Sensitivity lard Macronutrient Polyunsaturated Fatty Acids Pork Proteins salmon oil Saturated Fatty Acid Therapy, Diet
The control group (CON, n=8) was fed a standard ad libitum diet, consisting of dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]).
For the high-fat diet groups, food was presented from 9:00am to 10:00am each day. Animals were fed a daily diet of one can of Hill’s Prescription Diet (415g; 10% carbohydrate, 9% protein 8% fat, 0.3% fiber and 73% moisture [Hill’s Pet Nutrition, Topeka KS, USA]) and 825g dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]), supplemented with either 6g/kg of rendered pork fat (LARD, n=8) or salmon oil (SO, n=8). These alternative diets contained identical macronutrient content consisting of 21,025kJ/day comprised of 27% carbohydrate, 19% protein and 53% fat. The added lard consisted of 38.5% saturated fatty acid, 10.8% polyunsaturated fatty acid (10% ω-6; 0.8% ω-3), 44.6% monounsaturated fatty acid, and 0.1% cholesterol. The Salmon oil addition consists of 19.9% saturated fatty acid, 40.4% polyunsaturated fatty acid (1.5% ω-6; 35.5% ω-3).
The acute effects of six weeks of LARD and SO diets on insulin sensitivity and cardiac function have been reported previously (15 ). The present study reports data on extended diet durations of 3.0±0.3 months of lard feeding and 3.9±0.1 months of salmon oil feeding.
Publication 2017
Animals Canis familiaris Carbohydrates Cholesterol Diet Diet, High-Fat Fatty Acids, Monounsaturated Fibrosis Food Heart Insulin Sensitivity lard Macronutrient Polyunsaturated Fatty Acids Pork Proteins salmon oil Saturated Fatty Acid Therapy, Diet
Dogs were fed a standard daily diet of one can of Hill's Prescription Diet (415 g; 10% carbohydrate, 9% protein, 8% fat, 0.3% fibre and 73% moisture [Hill's Pet Nutrition, Topeka, KS, USA]) and 825 g dry chow (40% carbohydrate, 26% protein, 14% fat, and 3% fibre [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond, IN, USA]) for 2 weeks prior to participation in the study. Food was presented from 9:00 to 10:00 each day and consisted of 14,970 kJ/day: 38% from carbohydrates, 26% from protein and 36% from fat. Following baseline experiments, animals were randomly assigned to a control, lard or salmon oil diets, on which they remained for the duration of the study. Animal batches were randomly assigned to dietary groups using the random number generator in Microsoft Excel. The control group remained on the standard diet (n=6). For the high-fat diet groups, the standard diet was supplemented with 6 g/kg of either rendered pork fat (lard, n=12) or salmon oil (n=8), resulting in high-fat diets of identical macronutrient content consisting of 21,025 kJ/day comprised of 27% carbohydrate, 19% protein and 53% fat. Diets were given for 6 weeks, according to the standard lard-fed protocol used in our laboratory to develop diet-induced insulin resistance in this model. A schematic diagram of the protocol is shown in Fig. 1. Each animal underwent baseline assessments, which were repeated after 6 weeks. A subset of eight lard-fed animals was intermediately assessed after 2 weeks.
Publication 2015
Animals Canis familiaris Carbohydrates Diet Diet, High-Fat FED protocol Fibrosis Food Insulin Resistance lard Macronutrient Pork Proteins salmon oil Therapy, Diet

Most recents protocols related to «Insulin pork»

Mammary epithelial, non-transformed MCF10A cells (ATCC CRL-10317) were grown in DMEM:F-12 (Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12) (1:1) medium (Gibco, 11330057) supplemented with 5% Horse Serum (HS), 2 mM l-glutamine and 1% penicillin/streptomycin (Sigma-Aldrich, G6784), 10 µg/ml insulin (Sigma-Aldrich, I9278) and 0.5 µg/ml hydrocortisone (Sigma-Aldrich, H0888). Human Epidermal Growth Factor (hEGF, Sigma-Aldrich, E9644) was added freshly before the use of full medium (20 nm/ml). MCF7 cells and MDA-MB-231 cells (ATTC HTB-26) were grown in DMEM medium (Gibco, 11330057) supplemented with 10% Fetal Bovine Serum (FBS, Euroclone, ECS0180L), 1% penicillin/streptomycin (Sigma-Aldrich, G6784) and 2 mM l-glutamine. Cells were grown on 10 cm2 petri dishes for a month (about 10–12 cell passages) at 37 °C in 5% CO2. For any experiments on fixed cells, cells were plated on glass coverslips coated with 0.5% (w/v) pork gelatin (Sigma-Aldrich, G2500) previously dissolved in phosphate buffer saline (PBS) and autoclaved.
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Publication 2023
Breast Buffers Cells Eagle Epidermal growth factor Epithelial Cells Equus caballus Gelatins Glutamine Homo sapiens Hydrocortisone Hyperostosis, Diffuse Idiopathic Skeletal Insulin MCF-7 Cells MDA-MB-231 Cells Nutrients Penicillins Phosphates Pork Saline Solution Serum Streptomycin
Male C57BL/6 mice were maintained for 6 months on a high calorie diet (HCD) with high fat (35.8%) and high carbohydrate (36.8%), consisting of a standard pelleted feed, which was mixed with butter and sugar. Pork fat was given in addition to the feed to accelerate the development of obesity and impaired glucose tolerance. After the development of impaired glucose and insulin tolerance (after 6 months), animals were randomised by weight and divided into 4 groups (n = 7–13): (1) “Intact control”: vehicle (distilled water + 2 drops of Tween 80) and standard pelleted feed; (2) “Negative control”: vehicle (distilled water + 2 drops of Tween 80) + HCD; (3) “Positive control”: metformin at a dose of 250 mg/kg + HCD; (4) “Composition”: composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg + HCD. All compounds were given once a day by oral gavage for 4 weeks.
Body weight was recorded weekly, and an oral glucose tolerance test was performed after 4 weeks. At the end of experiment, mice were decapitated, blood was taken for biochemical examination, the liver and pancreas for histology.
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Publication 2023
Animals BLOOD Body Weight Butter Carbohydrates Cranberry Diet, High-Fat Glucose Immune Tolerance Insulin Intolerances, Glucose Leuzea Liver Males Metformin Mice, House Mice, Inbred C57BL Obesity Oral Glucose Tolerance Test Pancreas Pork Therapy, Diet Tube Feeding Tween 80
Mammary epithelial, non-transformed MCF10A cells (ATCC CRL-10317) were grown in DMEM:F-12 (Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12) (1 : 1) medium (Gibco, 11330057) supplemented with 5% Horse Serum (HS), 2 mM L-glutamine and 1% penicillin/streptomycin (Sigma-Aldrich, G6784), 10 µg/ml insulin (Sigma-Aldrich, I9278) and 0,5 µg/ml hydrocortisone (Sigma-Aldrich, H0888). Human Epidermal Growth Factor (hEGF, Sigma-Aldrich, E9644) was added freshly before the use of full medium (20 nm/ml). MDAMB231 cells (ATTC HTB-26) were grown in DMEM medium (Gibco, 11330057) supplemented with 10% Fetal Bovine Serum (FBS, Euroclone, ECS0180L), 1% penicillin/streptomycin (Sigma-Aldrich, G6784) and 2 mM L-glutamine. Cells were grown on 10cm 2 Petri Dish for a month (about 10-12 cell passages) at 37°C in 5% CO2. For any experiments on fixed cells, cells were plated on glass coverslips coated with 0,5% (w/v) pork gelatin (Sigma-Aldrich, G2500) previously dissolved in phosphate buffer saline (PBS) and autoclaved. For hyperacetylation experiments, MCF10A and MDAMB231 cells were treated with 300 nM of trichostatin A (TSA) (Sigma-Aldrich, T8552) in complete growth medium for 24h prior the fixation.
Publication 2023
A specialist collected data from medical records, including sex, age, phone number, height, and weight, as well as levels of alanine transaminase (ALT), aspartate transaminase (AST), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), SUA, HbA1c, creatinine, and estimated glomerular filtration rate [eGFR = 186 × (creatinine/88.4)−1.154 × (age)−0.203 × (1 (male) or 0.74 (female))]. Blood biochemical parameters were measured using an automatic biochemical analyzer, and HbA1c was detected using high-performance liquid chromatography (1366 cases) and affinity chromatography (211 cases). Laboratory tests of all participants were subject to quality control at the Department of Laboratory Medicine, West China Hospital, Sichuan University.
In addition, we recorded information regarding hypoglycemic, antihypertensive, lipid-lowering, antiplatelet, and uric acid-lowering drugs used at the time of blood collection, including sulfonylureas, thiazolidinedione, other insulin secretagogues, metformin, α-glucosidase inhibitors, dipeptidyl peptidase 4 inhibitors, glucagon-like peptide 1 (GLP-1) receptor agonists, sodium-glucose cotransporter 2 (SGLT-2) inhibitors, insulin, angiotensin-converting enzyme inhibitors (ACEIs), angiotensin II receptor blockers (ARBs), calcium channel blockers (CCBs), adrenergic α-antagonists, adrenergic β-antagonists, diuretics, statins, fibrates, aspirin, clopidogrel, benzbromarone, febuxostat, and allopurinol.
Finally, a well-trained interviewer collected information regarding lifestyle habits at the time of blood collection. We defined variables as follows: (1) current drinking: drinking at least once weekly in the last year19
; (2) current smoking: smoking in the past 30 days, including daily smoking and occasional smoking20
; (3) exercise: at least 150 min of moderate intensity physical activity, or at least 75 min of high-intensity physical activity weekly, or at least 150 min of moderate and high-intensity physical activities21 (link)
; (4) staying up late: sleeping after 11:00 pm greater than or equal to five times weekly22 (link)
; (5) regular lunch break: greater than or equal to three times weekly and ⩾30 min each time; (6) red meats: pork, beef, mutton, and rabbit meat; (7) fishes: fresh water fish; (8) fried foods: foods cooked in oil at a high temperature for rapid maturation; (9) seafood: edible sea-dwelling creatures; (10) strong teas: tea leaves accounting for half or more of the total volume after brewing23 (link)
; and (11) broths: chicken, duck, fish, bone, or rib soup.
Publication 2023
Mammary epithelial, non-transformed cell line MCF10A (ATCC CRL-10317) was grown in Dulbecco's modified Eagle's medium:nutrient mixture F-12 (DMEM:F-12; 1:1) medium (Gibco, 11330057) supplemented with 5% horse serum (HS, Thermo Fisher Scientific, 16050122), 2 mM L-glutamine (Sigma-Aldrich) and 1% penicillin-streptomycin (Sigma-Aldrich, G6784), 10 µg/ml insulin (Sigma-Aldrich, I9278), 0.5 µg/ml hydrocortisone (Sigma-Aldrich, H0888), and 20 ng/ml of human epidermal growth factor (hEGF, Sigma-Aldrich, E9644) added freshly before use. MCF7 cells and MDA-MB-231 cells (ATTC HTB-26) were grown in DMEM (Gibco, 11330057) supplemented with 10% fetal bovine serum (FBS, Euroclone, ECS0180L), 1% penicillin-streptomycin (Sigma-Aldrich, G6784) and 2 mM L-glutamine. Cells were grown on 10 cm2 dishes (about 10–12 cell passages) at 37°C in 5% CO2. For any experiments on fixed cells, cells were plated on glass coverslips coated with 0.5% (w/v) pork gelatine (Sigma-Aldrich, G2500) dissolved in phosphate-buffered saline (PBS) and autoclaved. U2-OS osteosarcoma (a kind gift from G. Marra, University of Zurich, Switzerland) and U2-OS KI eGFP-DEK cells (described in Ganz et al., 2019 (link)) were cultured in McCoy's 5a modified medium (Gibco, Life Technologies) supplemented with 10% FBS (Capricorn Scientific), 100 U/ml penicillin and 100 µg/ml streptomycin (both Gibco, Life Technologies). For selection after transfection, Geneticin (Thermo Fisher Scientific) was added at concentrations of 200 µg/ml for U2OS KI eGFP-DEK WT, eGFP-DEK SUMOmut Δ61,62, eGFP-DEK SUMOmut Δ261 and eGFP-DEK SUMOmut Δ61,62,144,145,261 cells. During all experiments using siRNA-mediated protein downregulation penicillin and streptomycin were omitted from the medium. HeLa S3 cervical adenocarcinoma cells and HeLa 6×His-SUMO cells (a kind gift from Ronald T. Hay, University of Dundee, UK) were cultured in DMEM (Gibco, Life Technologies) supplemented with 10% FBS (Capricorn Scientific), 100 U/ml penicillin, 100 μg/ml streptomycin (both Gibco, Life Technologies) and 6 mM L-glutamine (Gibco, Life Technologies). hTERT immortalized BJ-5ta foreskin fibroblasts were cultured in a 4:1 mixture of DMEM and Medium 199 (Gibco, Life Technologies) supplemented with 10% FBS, 4 mM L-glutamine and 10 µg/ml hygromycin B (Calbiochem).
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Publication 2023

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More about "Insulin pork"

Insulin pork, also known as porcine insulin, refers to the use of insulin derived from pork for the treatment of diabetes mellitus.
Pork insulin is structurally similar to human insulin and has been used as an effective alternative to bovine (cow-derived) insulin, particularly in cases where patients may be allergic or resistant to bovine insulin.
Research on insulin pork has focused on optimizing protocols, identifying best practices from literature and patents, and conducting data-driven comparisons to improve reproducibility and accuracy.
Tools like PubCompare.ai can enhance insulin pork research by helping researchers locate the most relevant and reliable information from scientific publications, preprints, and patents.
In addition to insulin pork, other related topics that may be of interest include Rapamycin (an immunosuppressant drug), Penicillin/Streptomycin (an antibiotic combination), LY303511 (a PI3K inhibitor), VeraCode GoldenGate Assay kit (a genotyping tool), Wizard Genomic DNA Purification Kit (a DNA extraction kit), Dexamethasone (a synthetic glucocorticoid), PD98059 (a MEK inhibitor), SP600125 (a JNK inhibitor), SB203580 (a p38 MAPK inhibitor), and Picropodophyllin (an IGF-1R inhibitor).
Understanding the interplay between these various compounds and techniques can provide valuable insights for researchers working in the field of insulin pork and diabetes treatment.