Type 2 diabetic rats were induced by the combination of a high-fat diet (HFD) and a low-dose STZ as described by Srinivasan
[16 (link)]. Male Sprague-Dawleys (SD) rats weighing 200–220 g were obtained from the Experimental Animal Center of the Hebei Medical University, Shijiazhuang, Hebei Province, China. After 1 week of acclimation, the rats were randomly divided into a control group and a challenge group, and they were fed a normal pellet diet (NPD) consisting of 10.3% fat, 24.2% protein and 65.5% carbohydrate and a HFD consisting of 59.8% fat (mainly pork), 20.1% protein and 20.1% carbohydrate. After 8 weeks of dietary manipulation, an oral glucose tolerance test (OGTT) was performed as per the literature method to confirm the insulin resistance (IR) of the challenge group rats
[17 (link)].
Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ; Alexis, USA; 25 mg/kg i.p. in a 0.1 mol/L citrate buffer, pH 4.5) given to rats with insulin resistance. The NPD rats (CON group) were given via the i.p. route in a citrate buffer vehicle. One week after STZ administration, rats with fasting blood glucose (FBG) ≥11.1 mmol/L in two consecutive analyses were considered to be the type 2 diabetic rat model (DM group). All animals remained on the assigned diet until the terminal experiment.
After 8 weeks of diabetes, the FBG was performed again: CON (5.0 ± 0.4 mmol/L) and DM (12.7 ± 1.9 mmol/L). Then, the rats were anaesthetized by using 20% urethane (1.2 g/kg intraperitoneally), and plasma (8–10 ml per animal) was immediately collected from the femoral artery and processed into serum. After being washed in ice-cold saline solution, the hearts were weighed and frozen in liquid nitrogen, then stored at −80°C. All experimental procedures were performed in accordance with the guidelines established by the Ethics Review Committee for Animal Experimentation (Hebei Medical University, Shijiazhuang, China).
[16 (link)]. Male Sprague-Dawleys (SD) rats weighing 200–220 g were obtained from the Experimental Animal Center of the Hebei Medical University, Shijiazhuang, Hebei Province, China. After 1 week of acclimation, the rats were randomly divided into a control group and a challenge group, and they were fed a normal pellet diet (NPD) consisting of 10.3% fat, 24.2% protein and 65.5% carbohydrate and a HFD consisting of 59.8% fat (mainly pork), 20.1% protein and 20.1% carbohydrate. After 8 weeks of dietary manipulation, an oral glucose tolerance test (OGTT) was performed as per the literature method to confirm the insulin resistance (IR) of the challenge group rats
[17 (link)].
Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ; Alexis, USA; 25 mg/kg i.p. in a 0.1 mol/L citrate buffer, pH 4.5) given to rats with insulin resistance. The NPD rats (CON group) were given via the i.p. route in a citrate buffer vehicle. One week after STZ administration, rats with fasting blood glucose (FBG) ≥11.1 mmol/L in two consecutive analyses were considered to be the type 2 diabetic rat model (DM group). All animals remained on the assigned diet until the terminal experiment.
After 8 weeks of diabetes, the FBG was performed again: CON (5.0 ± 0.4 mmol/L) and DM (12.7 ± 1.9 mmol/L). Then, the rats were anaesthetized by using 20% urethane (1.2 g/kg intraperitoneally), and plasma (8–10 ml per animal) was immediately collected from the femoral artery and processed into serum. After being washed in ice-cold saline solution, the hearts were weighed and frozen in liquid nitrogen, then stored at −80°C. All experimental procedures were performed in accordance with the guidelines established by the Ethics Review Committee for Animal Experimentation (Hebei Medical University, Shijiazhuang, China).
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