Transwell invasion assay was performed as described previously4 (link). In brief, cells were loaded onto the upper well of the Transwell chamber with 8 µm ϕ pore membrane (Coster), precoated with Matrigel on an upper side of the chamber. The lower well was filled with 600 µl of DMEM containing 10% FBS. After incubation for 24 hr, cells invaded to lower surface of the membrane were counted. For ECM degradation assay, glass coverslips were coated with gelatin conjugated with either Alexa Fluor 594 (Invitrogen) (Alexa-gelatin) or fluorescein (Invitrogen) (FL-gelatin) as described65 (link). Transfected cells were trypsinized, replated on these glass coverslips, and cultured for 6 hr. After fixation, cells were fixed and stained with phalloidin. Number of invadopodia, identified as F-actin dots in the areas of degraded gelatin, was quantified by using the ImageJ particle analysis tool.
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Matrigel
Matrigel
Matrigel is a gelatinous protein mixture that resembles the complex extracellular environment found in many tissues.
It is widely used in cell culture and tissue engineering applications, particularly in the study of angiogenesis, tumor cell invasion and metastasis, and stem cell differentiation.
PubCompare.ai provides a powerful AI-driven research platform to help scientists optimize their Matrigel protocols by easily locating the most reliable and reproducible methods from literature, preprints, and patents.
With accurate comparisons, this tool can streamline Matrigel research and identify the best approaches for your specific needs.
Discover how PubCompare.ai can enhance your Matrigel studies and contribute to more robust and reproducible findings.
It is widely used in cell culture and tissue engineering applications, particularly in the study of angiogenesis, tumor cell invasion and metastasis, and stem cell differentiation.
PubCompare.ai provides a powerful AI-driven research platform to help scientists optimize their Matrigel protocols by easily locating the most reliable and reproducible methods from literature, preprints, and patents.
With accurate comparisons, this tool can streamline Matrigel research and identify the best approaches for your specific needs.
Discover how PubCompare.ai can enhance your Matrigel studies and contribute to more robust and reproducible findings.
Most cited protocols related to «Matrigel»
Alexa594
Biological Assay
Cells
F-Actin
Fluorescein
Gelatins
matrigel
Phalloidine
Podosomes
Tissue, Membrane
hESC cultures were disaggregated using accutase for 20 minutes, washed using hESC media and pre-plated on gelatin for 1 hour at 37°C in the presence of ROCK inhibitor to remove MEFs. The nonadherent hESC were washed and plated on matrigel at a density of 10,000–25,000 cells/cm2 on matrigel (BD) coated dishes in MEF conditioned hESC media (CM) spiked with 10 ng/mL of FGF-2 and ROCK-inhibitor. Ideal cell density was found to be 18,000 cells/cm2. The ROCK inhibitor was withdrawn, and hESC were allowed to expand in CM for 3 days or until they were nearly confluent. The initial differentiation media conditions included knock out serum replacement (KSR) media with 10 nM TGF-b inhibitor (SB431542, Tocris) and 500 ng/mL of Noggin (R&D). Upon day 5 of differentiation, the TGF-b inhibitor was withdrawn and increasing amounts of N2 media (25%, 50%, 75%) was added to the KSR media every two days while maintaining 500 ng/mL of Noggin. For MS5 induction, established methods previously reported were used22 (link).
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
accutase
Cells
Culture Media, Conditioned
FGF10 protein, human
Fibroblast Growth Factor 2
Gelatins
Human Embryonic Stem Cells
Hyperostosis, Diffuse Idiopathic Skeletal
matrigel
noggin protein
Serum
Transforming Growth Factor beta
The reorientation of the centrosome during tumor invasion was assessed by 2D invasion assay. The two-well culture insert with 0.5 mm gap between wells (ibidi) was placed on a fibronectin-coated glass-bottom dish. SaOS2 cells transfected with the respective siRNAs were plated onto the culture insert and grown to confluent monolayers. After the inserts were removed, the monolayers were washed with PBS and overlaid with Matrigel (BD) diluted 1:20 in PBS, followed by incubation for 4 hr before addition of growth medium. Cells were then cultured for 24 hr to allow invasion toward the space between the monolayers. After fixation with 4% (w/v) paraformaldehyde, cells were stained with antibody to γ-tubulin to visualize the centrosome, and counterstained with DAPI. The percentages of the edge cells in which the centrosome was within the 120° sector emerging from the center of the nucleus and facing toward the space between the monolayers was measured.
Biological Assay
Centrosome
Cerebellar Nuclei
Culture Media
DAPI
FN1 protein, human
Hyperostosis, Diffuse Idiopathic Skeletal
Immunoglobulins
matrigel
Neoplasm Invasiveness
paraform
RNA, Small Interfering
Tubulin
Images were acquired by collaborators from the Allen Institute for Cell Science, Seattle, as per Roberts and colleagues [34 (link)]. Briefly, wild-type C (WTC) hiPSCs were cultured in a feeder-free system on tissue culture dishes or plates coated with GFR Matrigel (Corning) diluted 1:30 in cold DMEM/F12 (Gibco). Undifferentiated cells were maintained with phenol red containing mTeSR1 media (85850, STEMCELL Technologies) supplemented with 1% (v/v) penicillin-streptomycin (P/S; Gibco). Cells were not allowed to reach confluency greater than 85% and are passaged every 3–4 days by dissociation into single-cell suspension using StemPro Accutase (Gibco). When in single-cell suspension, cells were counted using a Vi-CELL Series Cell Viability Analyzer (Beckman Coulter). After passaging, cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Stemolecule Y-27632, Stemgent) for 24 hours. Media is replenished with fresh mTeSR1 media supplemented with 1% P/S daily. Cells were maintained at 37°C and 5% CO2. Cells were maintained with phenol red–free mTeSR1 media (05876, STEMCELL Technologies) 1 day prior to live cell imaging.
Three to four days after cells are plated and mature and healthy colonies are observed on 96- and 24-well imaging plates, the cells are stained with NucBlue Live ready probe reagent (R37605, ThermoFisher) and CellMask Deep Red plasma membrane stain (C10046, ThermoFisher) to visualize DNA and plasma membrane, respectively. The protocol is available online:http://www.allencell.org/uploads/8/1/9/9/81996008/sop_for_cellmask-and-nucblue_v1.0_1.pdf . Phenol red–free mTeSR1 is preequilibrated to 37°C and 5% CO2. 1X NucBlue solution made in preequilibrated phenol red–free mTeSR1 is spun for 60 minutes at 20,000 g. The 2X and 10X working stocks of CellMask Deep Red lot #1730970 and #1813792, respectively, are made in 1X NucBlue solution. All solutions are kept at 37°C and 5% CO2 until used. The 100 μL and 400 μL of NucBlue solution are added per well of 96-well imaging plates and 24-well imaging plates, respectively, and incubated at 37°C and 5% CO2 for 20 minutes. An equal amount of CellMask Deep Red working stock is added to the wells containing NucBlue solution. Final dye concentrations in the wells are 1X NucBlue and 1X and 5X CellMask Deep Red lots #1730970 and #1813792, respectively. Cells are incubated at 37°C and 5% CO2 for 10 minutes and gently washed with preequilibrated phenol red–free mTeSR1. Fields of view as shown in Fig 4 that are acquired near the edge (and the center as a control) of hiPSC colonies receive an additional photoprotective cocktail treatment which serves to minimize singlet oxygen and free radical formation. The photoprotective cocktail is used at a working concentration of 0.3 U/ml (1:100) OxyFluor as defined by the OxyFluor product insert, with the addition of 10 mM sodium lactate and 1 mM ascorbic acid (OxyFluor OF-0005, Oxyrase).
As per Roberts and colleagues [34 (link)], cells were imaged on a Carl Zeiss spinning disk microscope with a Carl Zeiss 20×/0.8 NA plan APOCHROMAT or 100×/1.25 W C-APOCHROMAT Korr UV Vis IR objective, a CSU-X1 Yokogawa spinning disk head, and Hamamatsu Orca Flash 4.0 camera. Microscopes were outfitted with a humidified environmental chamber to maintain cells at 37°C with 5% CO2 during imaging. Cells are imaged immediately following the wash step and for up to 2.5 hours after dye addition on a Zeiss spinning disk microscope at 100× with the following general settings: 405 nm at 0.28 mW, 200 ms exposure; 638 nm at 2.4 mW, 200 ms exposure; acquiring each channel at each z-step.
Three to four days after cells are plated and mature and healthy colonies are observed on 96- and 24-well imaging plates, the cells are stained with NucBlue Live ready probe reagent (R37605, ThermoFisher) and CellMask Deep Red plasma membrane stain (C10046, ThermoFisher) to visualize DNA and plasma membrane, respectively. The protocol is available online:
As per Roberts and colleagues [34 (link)], cells were imaged on a Carl Zeiss spinning disk microscope with a Carl Zeiss 20×/0.8 NA plan APOCHROMAT or 100×/1.25 W C-APOCHROMAT Korr UV Vis IR objective, a CSU-X1 Yokogawa spinning disk head, and Hamamatsu Orca Flash 4.0 camera. Microscopes were outfitted with a humidified environmental chamber to maintain cells at 37°C with 5% CO2 during imaging. Cells are imaged immediately following the wash step and for up to 2.5 hours after dye addition on a Zeiss spinning disk microscope at 100× with the following general settings: 405 nm at 0.28 mW, 200 ms exposure; 638 nm at 2.4 mW, 200 ms exposure; acquiring each channel at each z-step.
GCaMP variants were made in a modified SIV-based lentiviral construct, pGP-syn-GCaMP-nls-mCherry-WPRE, derived from pCL20cSLFR MSCV-GFP51 (link). The prolentiviral vector included a 476-bp human synapsin promoter, GCaMP, a nuclear localization sequence fused to mCherry, and the woodchuck hepatitis post-transcriptional regulatory element. Site-directed mutagenesis was conducted by PCR and mutated regions were incorporated into the lentiviral constructs by gene assembly52 (link).
Hippocampi were dissected and dissociated in papain. Cells were plated at a density of 225,000 viable cells/well in 24-well glass-bottom plates (Mattek, #1.5 glass coverslips), pre-coated with Matrigel (BD Biosciences). Cells were cultured in growth medium (28 mM glucose, 2.4 mM sodium bicarbonate, 100 μg/mL transferrin, B-27 supplement (1X, Invitrogen), 500 μM L-glutamine, 50 units/mL penicillin, 50 mg/mL streptomycin, 5% fetal bovine serum in MEM).
Lentiviral particles were made in a biosafety level 2 laboratory by transfecting a prolentiviral construct and packaging and coat pseudotyping DNA constructs (pCAG-SIVgprre, pCAG4-RTR-SIV, pCMV-VSV-G)51 (link),53 (link) into HEK293T/17 cells (ATCC) in 10-cm plates. After 72 h, supernatant was collected (6 mL) and filtered. Neuronal cultures were infected at 3 days in vitro. Each well of a 24-well plate was incubated overnight with 0.5 mL of lentivirus in conditioned growth medium. The growth medium was supplemented with 4 μM AraC to inhibit glial proliferation. In some experiments, OGB1-AM was loaded into cells by incubating neurons in 1 mL of 2 μM OGB1-AM (Invitrogen) for 30 min and rinsing 3 times with imaging buffer (145 mM NaCl, 2.5 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4, 2 mM CaCl2, 1 mM MgCl2).
Neurons were stimulated in imaging buffer containing a drug cocktail to inhibit synaptic receptors (10 μM CNQX, 10 μM (R)-CPP, 10 μM gabazine, 1 mM (S)-MCPG, Tocris). Under these conditions, intracellular calcium increases are presumably caused by the opening of voltage sensitive calcium channels.
Action potentials (APs) (83 Hz) were evoked by field stimulation with a Grass Technologies S48 stimulation unit and a custom-built 24-well cap stimulator with pairs of parallel platinum wires. The microscope was an Olympus IX81 with a 10× (0.4 NA) air objective lens and EMCCD camera (Andor 897, 512 × 512 pixels, 35 frames/s), Cairn OptoLED illumination system, and GFP (Excitation: 450-490 nm; Dichroic: 495 nm long-pass; Emission: 500-550 nm) and TxRed (Excitation: 540-580 nm; Dichroic: 585 nm long-pass; Emission: 593-668 nm) filter sets. The field of view was 800 μm × 800 μm. Images were background subtracted (mean of 5% lowest pixel values). Responses were quantified for each cell as change in fluorescence divided by baseline fluorescence measured one second prior to stimulation. Signal-to-noise ratio (SNR) was quantified as peak ΔF/F0 response over the standard deviation of the signal during a one second period prior to stimulation.
Control experiments varying stimulation voltage, frequency, and pulse width insured suprathreshold stimulation of neurons. Voltage imaging using the ArchWT-GFP archaerhodopsin-based voltage sensor54 (link) confirmed that individual pulses (1 ms, 40 V, 83 Hz) reliably triggered single APs. The imaging and stimulation system was controlled by custom scripts written in MetaMorph software (version 7.7.5, Molecular Devices) and Ephus software55 (link) (ephus.org). Detailed neuronal culture screening methods will be described elsewhere (T.J.W., T.W.C., E.R.S., R.A.K., V.J., L.L.L., K.S., and D.S.K., manuscript in preparation).
Hippocampi were dissected and dissociated in papain. Cells were plated at a density of 225,000 viable cells/well in 24-well glass-bottom plates (Mattek, #1.5 glass coverslips), pre-coated with Matrigel (BD Biosciences). Cells were cultured in growth medium (28 mM glucose, 2.4 mM sodium bicarbonate, 100 μg/mL transferrin, B-27 supplement (1X, Invitrogen), 500 μM L-glutamine, 50 units/mL penicillin, 50 mg/mL streptomycin, 5% fetal bovine serum in MEM).
Lentiviral particles were made in a biosafety level 2 laboratory by transfecting a prolentiviral construct and packaging and coat pseudotyping DNA constructs (pCAG-SIVgprre, pCAG4-RTR-SIV, pCMV-VSV-G)51 (link),53 (link) into HEK293T/17 cells (ATCC) in 10-cm plates. After 72 h, supernatant was collected (6 mL) and filtered. Neuronal cultures were infected at 3 days in vitro. Each well of a 24-well plate was incubated overnight with 0.5 mL of lentivirus in conditioned growth medium. The growth medium was supplemented with 4 μM AraC to inhibit glial proliferation. In some experiments, OGB1-AM was loaded into cells by incubating neurons in 1 mL of 2 μM OGB1-AM (Invitrogen) for 30 min and rinsing 3 times with imaging buffer (145 mM NaCl, 2.5 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4, 2 mM CaCl2, 1 mM MgCl2).
Neurons were stimulated in imaging buffer containing a drug cocktail to inhibit synaptic receptors (10 μM CNQX, 10 μM (R)-CPP, 10 μM gabazine, 1 mM (S)-MCPG, Tocris). Under these conditions, intracellular calcium increases are presumably caused by the opening of voltage sensitive calcium channels.
Action potentials (APs) (83 Hz) were evoked by field stimulation with a Grass Technologies S48 stimulation unit and a custom-built 24-well cap stimulator with pairs of parallel platinum wires. The microscope was an Olympus IX81 with a 10× (0.4 NA) air objective lens and EMCCD camera (Andor 897, 512 × 512 pixels, 35 frames/s), Cairn OptoLED illumination system, and GFP (Excitation: 450-490 nm; Dichroic: 495 nm long-pass; Emission: 500-550 nm) and TxRed (Excitation: 540-580 nm; Dichroic: 585 nm long-pass; Emission: 593-668 nm) filter sets. The field of view was 800 μm × 800 μm. Images were background subtracted (mean of 5% lowest pixel values). Responses were quantified for each cell as change in fluorescence divided by baseline fluorescence measured one second prior to stimulation. Signal-to-noise ratio (SNR) was quantified as peak ΔF/F0 response over the standard deviation of the signal during a one second period prior to stimulation.
Control experiments varying stimulation voltage, frequency, and pulse width insured suprathreshold stimulation of neurons. Voltage imaging using the ArchWT-GFP archaerhodopsin-based voltage sensor54 (link) confirmed that individual pulses (1 ms, 40 V, 83 Hz) reliably triggered single APs. The imaging and stimulation system was controlled by custom scripts written in MetaMorph software (version 7.7.5, Molecular Devices) and Ephus software55 (link) (ephus.org). Detailed neuronal culture screening methods will be described elsewhere (T.J.W., T.W.C., E.R.S., R.A.K., V.J., L.L.L., K.S., and D.S.K., manuscript in preparation).
Most recents protocols related to «Matrigel»
Corning® Cell Recovery Solution enabled us to retrieve cells from Matrigel®. Matrigel®-embedded samples were placed at 4°C for 30 min allowing Matrigel® to be liquified. All samples were transferred into new tubes with existent growth media and centrifuged at 1,200 rpm for 3 min at 4°C, then the supernatant was removed leaving only the Matrigel® layer including cells. An appropriate amount of Cell Recovery Solution (CORNING, Cat #354253) was next added to the Matrigel®/cell mix (the amount of the solution was recommended to be ≧2x that of Matrigel volume), which was then incubated at 4°C overnight. Cells were centrifuged at 1,200 rpm for 3 min at 4°C, and supernatant was aspirated. 1 mL of cold (4°C) PBS was added to the remaining cell pellet, mixed gently, then transferred into a microtube and centrifuged at 5,000 rpm for 3 min. After discarding the supernatant, cells were washed with cold PBS. Finally, cells were centrifuged at 15,000 rpm for 15 min, the supernatant discarded, and purified cell pellet was collected.
Prepare matrigel and add it to the lower hole of the ibidi angiogenesis slide. Put the matrigel glue into the incubator to make it coagulate. Prepare a cell suspension with a density of 2 × 10 5 cells/mL, and add 50 μL of the cell suspension to the solidified ibidi angiogenesis slide. According to the growth rate of the cells, images are collected periodically for observation.
The yellow tips were pre-cooled, and a 12/24 well plate along with Matrigel matrix (356254, Corning, BD Biosciences) was prepared in advance by storing them in a refrigerator at 4℃ for one day. Each well of the 24-well plates was uniformly coated with 200-300 μl of cold Matrigel, followed by incubation at 37℃ for half an hour to allow Matrigel solidification. Meanwhile, HUVEC were enzymatically dissociated using a 0.25% Typsin-EDTA solution. Subsequently, 2 × 105cells/well of HUVEC were exposed to 200 μM H2O2, 20 μg/ml ADSC-Exo, 100 nM mir-486-3p mimics and 200 nM mir-486-3p inhibitors. Photographic documentation was performed at time points of 2 h, 4 h, 6 h to observe the formation of tubular structures. The quantity analysis of branch points and the length of tube was quantified through Image J software (https://imagej.net/ij/ ) with blood vessels plug-in.
To study the cellular invasion towards chemo-attractant using a transwell migration assay, 100 μL of Matrigel (Corning, Corning, NY, USA) at 1 mg/mL was added to the migration chambers (8 microns, BD, San Jose, CA, USA) and allowed to stabilize at room temperature for 30 min. To analyze the invasion, 50,000 cells were seeded on the Matrigel in a 5% FBS medium and left to migrate toward a 10% FBS medium for 48 to 72 h depending on the cell line characteristics. To detect the migrated cells, the Matrigel was removed from the chambers, cells were fixed with 7% paraformaldehyde (Sigma), washed with PBS, and stained with cristal violet (Sigma). Migrated cells were counted from the high-power microscope fields (Leica DMI3000B microscope and Leica Application Suite camera software, Leica Application Suite X 1.1.0.12420, Wetzlar, Germany). Naïve counterpart fibroblasts isolated from the same patients were used as TAFs as controls.
Transwell chambers with 8 μm pores were used. The membrane was precoated with 50 μL Matrigel. After 24 h, inserts were fixed with methanol and stained with hematoxylin. The number of migrated cells was counted using a light microscope.
Top products related to «Matrigel»
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Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
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Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
Sourced in United States, China, United Kingdom, Germany, Switzerland, Japan, Australia
Transwell chambers are a type of lab equipment used for cell culture and biological assays. They consist of a permeable membrane insert placed inside a well, allowing for the study of cell-cell interactions and the movement of molecules across a barrier. The core function of Transwell chambers is to provide a controlled environment for culturing cells and monitoring their behavior and permeability.
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Matrigel is a complex mixture of extracellular matrix proteins and growth factors derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is commonly used as a substrate for the culture of various cell types, including epithelial, endothelial, and stem cells. Matrigel provides a physiologically relevant environment that supports cell attachment, growth, and differentiation.
Sourced in United States, Germany, United Kingdom, Japan, Canada, China, France, Italy
Matrigel matrix is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm mouse sarcoma cells. It provides a protein-rich, gelatinous, and biologically active microenvironment that supports the growth and differentiation of various cell types.
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Crystal violet is a synthetic dye commonly used in laboratory settings. It is a dark purple crystalline solid that is soluble in water and alcohol. Crystal violet has a variety of applications in the field of microbiology and histology, including as a staining agent for microscopy and in the gram staining technique.
Sourced in United States, United Kingdom, Germany, France, China, Canada, Japan, Belgium, Switzerland, Italy, Australia
Matrigel matrix is a complex mixture of extracellular matrix proteins and growth factors derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It provides a substrate that mimics the natural extracellular environment, supporting cell attachment, growth, migration, and differentiation.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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The BD BioCoat Matrigel Invasion Chambers are a laboratory equipment used to assess the invasive potential of cells. The chambers are pre-coated with Matrigel basement membrane matrix, which acts as a barrier to mimic the extracellular matrix. Researchers can use these chambers to study the ability of cells to invade through this barrier.
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Growth factor-reduced Matrigel is a complex extracellular matrix (ECM) preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is a soluble basement membrane extract that solidifies to form a gel-like material when warmed to physiological temperature.
More about "Matrigel"
Matrigel is a widely used gelatinous protein mixture that mimics the complex extracellular environment found in many tissues.
This basement membrane extract is commonly employed in cell culture and tissue engineering applications, particularly for studying angiogenesis (the formation of new blood vessels), tumor cell invasion and metastasis, and stem cell differentiation.
PubCompare.ai is a powerful AI-driven research platform that can help scientists optimize their Matrigel protocols.
This tool allows users to easily locate the most reliable and reproducible Matrigel methods from literature, preprints, and patents, while providing accurate comparisons to identify the best approaches for their specific needs.
In addition to Matrigel, related terms and concepts that are relevant include Transwell chambers, which are often used in conjunction with Matrigel to study cell migration and invasion, and the Matrigel matrix, a three-dimensional scaffold that can be used to culture cells in a more physiologically relevant environment.
Crystal violet, a dye commonly used to stain and quantify cells, is another related term.
FBS (Fetal Bovine Serum) is a common supplement added to cell culture media, including when working with Matrigel.
BD BioCoat Matrigel Invasion Chambers are a commercially available product that combines Matrigel with a Transwell system to study cell invasion.
Additionally, Growth factor-reduced Matrigel is a specialized version of the matrix that contains lower levels of growth factors, which can be useful for certain applications.
By utilizing the insights and capabilities of PubCompare.ai, scientists can streamline their Matrigel research, identify the most reliable and reproducible methods, and contribute to more robust and reproducible findings in their studies.
This basement membrane extract is commonly employed in cell culture and tissue engineering applications, particularly for studying angiogenesis (the formation of new blood vessels), tumor cell invasion and metastasis, and stem cell differentiation.
PubCompare.ai is a powerful AI-driven research platform that can help scientists optimize their Matrigel protocols.
This tool allows users to easily locate the most reliable and reproducible Matrigel methods from literature, preprints, and patents, while providing accurate comparisons to identify the best approaches for their specific needs.
In addition to Matrigel, related terms and concepts that are relevant include Transwell chambers, which are often used in conjunction with Matrigel to study cell migration and invasion, and the Matrigel matrix, a three-dimensional scaffold that can be used to culture cells in a more physiologically relevant environment.
Crystal violet, a dye commonly used to stain and quantify cells, is another related term.
FBS (Fetal Bovine Serum) is a common supplement added to cell culture media, including when working with Matrigel.
BD BioCoat Matrigel Invasion Chambers are a commercially available product that combines Matrigel with a Transwell system to study cell invasion.
Additionally, Growth factor-reduced Matrigel is a specialized version of the matrix that contains lower levels of growth factors, which can be useful for certain applications.
By utilizing the insights and capabilities of PubCompare.ai, scientists can streamline their Matrigel research, identify the most reliable and reproducible methods, and contribute to more robust and reproducible findings in their studies.