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MG 132

MG 132 is a potent and selective proteasome inhibitor that has become a widely used tool in biomedical research.
It functions by blocking the proteolytic activity of the 26S proteasome, a large multi-subunit complex responsible for the degradation of ubiquitin-tagged proteins.
The inhibition of the proteasome by MG 132 leads to the accumulation of these proteins, which can be used to study their functions and signaling pathways.
MG 132 has been employed in a variety of experimental settings, including cell culture studies, animal models, and clinical trials, to investigate its effects on cellular processes such as apoptosis, cell cycle regulation, and immune response.
Researchers can optimzie their MG 132 studies by leveraging the AI-driven platform PubCompare.ai, which enhnaces reproducibility and accuracy by facilitating the identification of the best protocols and products for their research needs.

Most cited protocols related to «MG 132»

1
Protein Extraction and Immunoblot Analysis
Total proteins were extracted from 100 mg of sample using extraction buffer (100 mM Tris-Cl pH8, 150 mM NaCl, 0.6% IGEPAL, 1 mM EDTA, 3 mM DTT with protease inhibitors, PMSF, leupeptin, aprotinin, pepstatin, antipain, chymostatin, Na2VO3, NaF, MG132, and MG115. Proteins were separated on a 10% polyacrylamide gel. Immunoblot analysis was carried out using mouse α-GFP (1:2000; Invitrogen) for TuMV GFP and rat α-HA (1:500) antibody for pCas13a. The antigens were detected by chemiluminescence using an ECL-detecting reagent (Thermo Scientific).
Aman R., Ali Z., Butt H., Mahas A., Aljedaani F., Khan M.Z., Ding S, & Mahfouz M. (2018). RNA virus interference via CRISPR/Cas13a system in plants. Genome Biology, 19, 1.
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Publication 2018
Antigens Antipain Aprotinin Buffers Chemiluminescence chymostatin Edetic Acid Elafin Immunoblotting Immunoglobulins leupeptin MG 115 MG 132 Mice, House pepstatin polyacrylamide gels Proteins Sodium Chloride Tromethamine
2
Genetic Engineering of hMps1 Mutants
hMps1 was PCR amplified from an expressed sequence tag (IMAGE No. 0511705), cloned into a pcDNA5/FRT/TO vector (Invitrogen) modified to contain an N-terminal GFP tag, and mutagenized (QuikChange; Stratagene) to create the D664A-, M602A-, and RNAi-resistant alleles (Table S1, available at http://www.jcb.org/cgi/content/full/jcb.200712028/DC1). Vectors were then cotransfected into Flp-In TRex tetracycline transactivator HeLa cells with the Flp recombinase encoding plasmid pOG44 as described previously (Tighe et al., 2004 (link)). Hygromycin-resistant colonies were pooled and expanded, and transgene expression was induced with 100 ng/ml tetracycline (Sigma-Aldrich). Nocodazole and taxol (both obtained from Sigma-Aldrich) were used at final concentrations of 0.2 μg/ml and 10 μM, respectively. 1NM-PP1 and SP600125 (both purchased from EMD) were used at 10 μM. MG132 (EMD) was used at 20 μM.
Tighe A., Staples O, & Taylor S. (2008). Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores. The Journal of Cell Biology, 181(6), 893-901.
Publication 2008
1-tert-butyl-3-naphthalen-1-ylmethyl-1H-pyrazolo(3,4-d)pyrimidin-4-ylemine Alleles Cloning Vectors Expressed Sequence Tags FLP recombinase HeLa Cells hygromycin A MG 132 Nocodazole Plasmids RNA Interference SP600125 Taxol Tetracycline Trans-Activators Transgenes
3
Cell Culture and Transfection Protocol

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Publication 2012
2-Mercaptoethanol Cells Cycloheximide Fetal Bovine Serum Gelatins Glutamine Human Embryonic Stem Cells leptomycin B MG 132 Mouse Embryonic Stem Cells Penicillins Streptomycin Sulfoxide, Dimethyl Tissues Training Programs Transfection
4
Ubiquitin Pulldown from PC3 Cells

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Publication 2015
Buffers Cells Guanidine imidazole Lanugo MG 132 PC 3 Cell Line Proteins SDS-PAGE Transfection Tromethamine
5
Construction and Analysis of Bub1 Mutant Cell Lines
Stable HeLa Flp-In, hTERT-RPE1 Flp-In, and Bub1 mutant cell lines were constructed according to the manufacturer’s protocol (Invitrogen). We amplified the Bub1 cDNA by PCR and inserted it into the pcDNA5/FRT/V5-His-TOPO vector (Invitrogen). Flag-EGFP was subcloned C-terminally of Bub1. The mutants were constructed via site-directed mutagenesis. Cells were grown at 37°C in 5% CO2 in either Dulbecco’s minimum essential medium + 10% FCS (HeLa cells) or 50:50 Ham’s F-12/DME + 10% FCS (hTERT-RPE1 cells). HeLa Kyoto H2B-mRED cells (provided by D. Gerlich, ETH Zurich, Zurich, Switzerland) were supplemented with 500 µg/ml G418, HeLa Flp-In, and hTERT-RPE1 Flp-In cells with 400 µg/ml zeocin, and stable Bub1 mutant cells were supplemented with 300 µg/ml hygromycine (HeLa) or 5 µg/ml puromycine (RPE1). Cells were transfected as described with 30- (HeLa) or 40-nM (RPE1) siRNAs (Bub1 siRNA, 5′-GAGUGAUCACGAUUUCUAA-3′; alternative Bub1 siRNA, 5′-AAGATGCATTTGAAGCCCAGT-3′) and analyzed 48 h after transfection (Elbashir et al., 2001 (link)). Cells were treated for 1 h with 1 µM MG132 prior to fixation to measure congression efficiency. To measure spindle checkpoint activity, cells were treated with 1 nM nocodazole for 16 h, and the fraction of rounded-up cells was determined by phase-contrast microscopy. To measure apoptosis, cells were incubated with 1 nM nocodazole for 16 h and fixed with 3.7% formaldehyde in phosphate-buffered saline, pH 7.4. A TUNEL assay was performed using an in situ cell death detection system that contained fluorescein–deoxy UTP (dUTP; Roche).
Klebig C., Korinth D, & Meraldi P. (2009). Bub1 regulates chromosome segregation in a kinetochore-independent manner. The Journal of Cell Biology, 185(5), 841-858.
Publication 2009
antibiotic G 418 Apoptosis Biological Assay BUB1 protein, human Cell Cycle Checkpoints Cell Death Cell Lines Cells Cloning Vectors deoxyuridine triphosphate DNA, Complementary Fluorescein Formaldehyde HeLa Cells In Situ Nick-End Labeling MG 132 Microscopy, Phase-Contrast Mutagenesis, Site-Directed Nocodazole Phosphates Puromycin RNA, Small Interfering Saline Solution Topotecan Transfection Zeocin

Most recents protocols related to «MG 132»

1
Ubiquitination Assay for Nrf2

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Publication 2023
Biological Assay Buffers Cells G-substrate MG 132 NFE2L2 protein, human Radioimmunoprecipitation Assay Ubiquitination Western Blot
2
Proteasome Activity Assay Protocol
The proteasome activity of vehicle and ASR490-treated ALDH+ cells was measured using a proteasome activity assay kit (BioVision) per the manufacturer’s protocol. MG132 was used as the positive control.
Saran U., Chandrasekaran B., Tyagi A., Shukla V., Singh A., Sharma A.K, & Damodaran C. (2023). A small molecule inhibitor of Notch1 modulates stemness and suppresses breast cancer cell growth. Frontiers in Pharmacology, 14, 1150774.
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Publication 2023
Biological Assay Cells MG 132 Multicatalytic Endopeptidase Complex
3
ALDH+, CD44+/CD24- Cell Viability
ALDH−, ALDH+, and CD44+/CD24− cells were first treated with vehicle control (DMSO) or treatment (ASR490, DAPT, MG132, or CQ) for prescribed doses and time points. Cell viability assays: Alamar blue (Life Technologies Corporation Eugene, OR) and EdU Cell Proliferation (using the EdU-Click 488 kit, cat# BCK-EdU488-1, Sigma), were then performed per the manufacturer’s instructions.
Saran U., Chandrasekaran B., Tyagi A., Shukla V., Singh A., Sharma A.K, & Damodaran C. (2023). A small molecule inhibitor of Notch1 modulates stemness and suppresses breast cancer cell growth. Frontiers in Pharmacology, 14, 1150774.
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Publication 2023
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol Alamar Blue Biological Assay CD44 protein, human Cell Proliferation Cells Cell Survival GIT1 protein, human MG 132 Sulfoxide, Dimethyl
4
Immortalized Breast Cell Culture
Human mammary immortalized cells (MCF10A), and the TNBC cell line MDA-MB-231 were purchased from American Type Culture Collection. MCF10A cells were grown in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s F12 medium with 20 ng mL−1 human epidermal growth factor, 100 ng mL−1 cholera toxin, 0.01 mg mL−1 bovine insulin, 500 ng mL−1 hydrocortisone, and 5% horse serum. MDA-MB-231 cells were grown in DMEM containing L-glutamine and sodium pyruvate, supplemented with 10% fetal bovine serum and 1% antibiotic and antimycotic solution in a humidified atmosphere of 5% CO2 at 37°C in an incubator. Human BCSC cells: ALDH+ and CD44+/CD24−, and BC cells: ALDH- were purchased from Celprogen (San Pedro, CA, United States) and maintained in human BCSC expansion and undifferentiation media. DAPT (γ-secretase), cycloheximide (CHX), chloroquine (CQ), and MG132 were purchased from Sigma (St. Louis, MO).
Saran U., Chandrasekaran B., Tyagi A., Shukla V., Singh A., Sharma A.K, & Damodaran C. (2023). A small molecule inhibitor of Notch1 modulates stemness and suppresses breast cancer cell growth. Frontiers in Pharmacology, 14, 1150774.
Full text: Click here
Publication 2023
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol Antibiotics Atmosphere Bos taurus Breast CD44 protein, human Cell Culture Techniques Cell Lines Cells Chloroquine Cholera Toxin Cycloheximide Eagle Epidermal growth factor Equus caballus Fetal Bovine Serum Glutamine Homo sapiens Hydrocortisone Insulin MDA-MB-231 Cells MG 132 Pyruvate Secretase Serum Sodium
5
Dissecting Notch Signaling Pathways
Cell lysates of ALDH−, ALDH+, and CD44+/CD24− cells following treatment with vehicle and treatment (ASR490, DAPT, MG132, CHX, or CQ) for prescribed doses and time points, were prepared with RIPA buffer (Thermo Scientific, Rockford, IL, United States) per the manufacturer’s protocol. Western blotting was performed using specific antibodies against Notch1 (CST, #3608), HES1 (Sigma, #SAB2108472), Hey1 (Proteintech, #19929-1-AP), NFκB p65 (CST, #8242), Bcl-2 (CST, #15071), Bcl-xL (CST, #2764), Vimentin (CST, #46173), Slug (CST, #9585), E-Cadherin (CST, #3195), β-catenin (CST, #8480), Ubiquitin (CST, #3933), Cleaved-PARP (CST, #5625), Cleaved-caspase-9 (CST, #20750), BAX (CST, #41162), Notch2 (CST, #D76A6), Lamp1 (CST, #9091), and LC3B (Proteintech, #14600-1-AP). β-Actin (CST, #4970) was used as the loading control. Protein bands were visualized using the Bio-Rad ChemiDocTM imaging system. For IP experiments, protein samples were immunoprecipitated with Notch1 antibody as per the protocol described elsewhere (Chandrasekaran et al., 2020 (link)), and Western blots were performed with ubiquitin antibody.
Saran U., Chandrasekaran B., Tyagi A., Shukla V., Singh A., Sharma A.K, & Damodaran C. (2023). A small molecule inhibitor of Notch1 modulates stemness and suppresses breast cancer cell growth. Frontiers in Pharmacology, 14, 1150774.
Full text: Click here
Publication 2023
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol Actins Antibodies BCL2 protein, human beta-Catenin Buffers Caspase 9 CD44 protein, human CDH1 protein, human Cells Immunoglobulins lysosomal-associated membrane protein 1, human MG 132 NOTCH2 protein, human Proteins Radioimmunoprecipitation Assay Slugs Transcription Factor RelA Ubiquitin Vimentin Western Blot

Top products related to «MG 132»

1
Mg132 by Merck Group
Sourced in United States, Germany, China, United Kingdom, Macao, Sao Tome and Principe, France, Canada, Italy, Switzerland, Morocco, Belgium, Japan, Sweden, Australia, Austria, Israel, Spain
MG132 is a proteasome inhibitor, a type of laboratory reagent used in research applications. It functions by blocking the activity of the proteasome, a complex of enzymes responsible for the degradation of proteins within cells. MG132 is commonly used in cell biology and biochemistry studies to investigate the role of the proteasome in various cellular processes.
2
Cycloheximide (chx) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium
Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.
3
Mg132 by Selleck Chemicals
Sourced in United States, China, Germany, United Kingdom
MG132 is a proteasome inhibitor that reversibly binds to the chymotrypsin-like site of the 26S proteasome. It is commonly used in cell biology research to investigate the role of the ubiquitin-proteasome system in cellular processes.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Lithuania, Hong Kong, Argentina, Ireland, Austria, Israel, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
6
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
7
Chloroquine by Merck Group
Sourced in United States, Germany, United Kingdom, China, France, Macao, Sao Tome and Principe, Switzerland, Italy, Canada, Japan, Spain, Belgium, Israel, Brazil, India
Chloroquine is a laboratory chemical primarily used as a research tool in biochemical and cell biology applications. It is a white, crystalline solid that is soluble in water. Chloroquine is commonly used in experiments to study cellular processes, such as autophagy and endocytosis, by inhibiting the function of lysosomes. Its core function is to serve as a research reagent for scientific investigations, without making any claims about its intended use.
8
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
9
Mg132 by MedChemExpress
Sourced in United States, China
MG132 is a potent, cell-permeable, reversible, and selective proteasome inhibitor. It inhibits the chymotrypsin-like activity of the proteasome. MG132 is widely used in cell biology research.
10
Nocodazole by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Sao Tome and Principe, Canada, China, Switzerland, France, Poland, Macao, Australia
Nocodazole is a synthetic compound that acts as a microtubule-destabilizing agent. It functions by binding to and disrupting the polymerization of microtubules, which are essential components of the cytoskeleton in eukaryotic cells. This property makes Nocodazole a valuable tool in cell biology research for studying cell division, cell motility, and other cellular processes that rely on the dynamics of the microtubule network.

More about "MG 132"

MG-132 is a potent and selective proteasome inhibitor that has become a widely used tool in biomedical research.
This small molecule compound functions by blocking the proteolytic activity of the 26S proteasome, a large multi-subunit complex responsible for the degradation of ubiquitin-tagged proteins.
By inhibiting the proteasome, MG-132 leads to the accumulation of these proteins, which can be leveraged by researchers to study their functions and signaling pathways.
MG-132 has been employed in a variety of experimental settings, including cell culture studies, animal models, and even clinical trials, to investigate its effects on cellular processes such as apoptosis, cell cycle regulation, and immune response.
Researchers can optimize their MG-132 studies by utilizing the AI-driven platform PubCompare.ai, which enhances reproducibility and accuracy by facilitating the identification of the best protocols and products for their research needs.
In addition to MG-132, other commonly used compounds in biomedical research include Cycloheximide, a protein synthesis inhibitor, and Chloroquine, a lysosomotropic agent.
The cell culture media DMEM and the transfection reagent Lipofectamine 2000 are also frequently employed, while DMSO is often used as a solvent for various compounds.
By gaining a comprehensive understanding of MG-132 and its applications, as well as the broader landscape of tools and techniques used in biomedical research, scientists can optimize their experimental design and ensure the reproducibility and accuracy of their findings.
The AI-powered PubCompare.ai platform can be a valuable resource in this endeavor, helping researchers navigate the vast body of scientific literature and identify the most effective protocols and products for their specific research goals.