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Occludin

Occludin is a transmembrane protein that plays a crucial role in the formation and regulation of tight junctions between epithelial and endothelial cells.
It is essential for maintaining the integrity and permeability of these cellular barriers, which are vital for proper organ function and homeostasis.
Occludin research focuses on understanding its structure, function, and potential therapeutic applications in various disease conditions, such as cancer, inflammation, and neurological disorders.
PubCompare.ai's AI-driven platform can help optimize Occludin research by identifying and evaluating the best protocols from literature, preprints, and patents, ensuring reproducible and accurate findings.
Leverage this intuitive platform to locate the top Occludin research and products for your studies, and unlock new insights to advance your scientific discoveries.

Most cited protocols related to «Occludin»

1
Immunohistochemical Analysis of Tight Junctions and Cell Adhesion Molecules
Cited 12 times
Immunohistochemical analysis was performed as already described [54 (link)]. Sections were probed overnight with anti-zonula occludens (ZO) antibody (1:100; Millipore, Abingdon, UK) or anti-occludin antibody (1:100; Santa Cruz Biotechnology) or anti-intracellular adhesion molecule-1 (ICAM-1) (1:100; Santa Cruz Biotechnology) or anti-P-selectin (1:100; Santa Cruz Biotechnology). Sections were washed with phosphate-buffer saline (PBS) and incubated with peroxidase-conjugated bovine anti-mouse IgG, secondary antibody (1:2000 Jackson Immuno Research, WestGrove, Pennsylvania, USA). Specific labeling was provided with a biotin-conjugated goat anti-mouse IgG and avidin-biotin peroxidase complex (Vector Laboratories, Burlingame, CA, USA). Images were collected using a Leica DM6 (Milan, Italy) microscope. The histogram profile reports the positive pixel intensity value taken from a computer program.
Fusco R., Cordaro M., Siracusa R., D’Amico R., Genovese T., Gugliandolo E., Peritore A.F., Crupi R., Impellizzeri D., Cuzzocrea S, & Di Paola R. (2020). Biochemical Evaluation of the Antioxidant Effects of Hydroxytyrosol on Pancreatitis-Associated Gut Injury. Antioxidants, 9(9), 781.
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Publication 2020
anti-IgG Antibodies, Anti-Idiotypic Avidin Biotin Buffers Cattle Cell Adhesion Molecules Cloning Vectors Goat Immunoglobulins Microscopy Mus Occludin Peroxidase Phosphates Protoplasm Saline Solution SELP protein, human Tight Junctions
2
Comprehensive Intestinal Gene Expression Analysis
Cited 12 times
Total RNA was isolated from mucosa scraping samples using Clontech Total RNA isolation NucleoSpin® RNA II kit (Clontech Laboratories, Inc., CA, USA). One microgram of total RNA, 11mer oligo mix from Fluoresentric, and M-MLV Reverse Transcriptase (Life Technologies, Grand Island, NY, USA) were used to synthesize cDNA according to the manufacturers’ instructions. The relative mRNA levels of mucin 2 (MUC2), fatty acid-binding protein (FABP) 2, FABP6, interleukin (IL)-8, IL-1β, transforming growth factor (TGF)-β4, occludin, zonula occluden (ZO)-1, junctional adhesion molecule (JAM) 2, JAM3, catenin, tumor necrosis factor (TNF) α, Toll-like receptor (TLR) 2β, TLR4, and claudin 1 were measured by quantitative PCR using Applied Biosystems® SYBR® Green PCR Master Mix, the 7500 Fast Real-Time PCR System, and primers in Table 2. Results were expressed as the level relative to the corresponding housekeeping gene actin. All primers were verified for the efficiency and linearity of amplification.
Chen J., Tellez G., Richards J.D, & Escobar J. (2015). Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens. Frontiers in Veterinary Science, 2, 14.
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Publication 2015
Actins Catenins Claudin-1 DNA, Complementary FABP2 protein, human Genes, Housekeeping IL1B protein, human Interleukin-8 isolation Junctional Adhesion Molecule B Mucin-2 Mucous Membrane Occludin Oligonucleotide Primers Oligonucleotides RNA, Messenger RNA-Directed DNA Polymerase RNA II SYBR Green I Tight Junctions Toll-Like Receptors transforming growth factor beta4 Tumor Necrosis Factor-alpha
3
Quantifying Brain Capillary Protein Levels
Cited 11 times

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Publication 2010
Actins Antibodies Blood Vessel Brain Capillaries Claudin-5 Collagen Type IV Densitometry Laminin Membrane, Basement Microvessels Occludin Plasma Proteins Plasmin Proteins Thrombin Tight Junction Proteins
4
Immunohistochemical Analysis of Intestinal Tight Junctions
Cited 9 times
At 8 days after the S. enterica and E. hirae infections, intestinal tissues were fixed in 10% (w/v) PBS-buffered formaldehyde and 7 μm sections were prepared from paraffin embedded tissues. Immunohistochemical localization was performed as previously described [42 (link)]. Sections were incubated overnight with (1) purified goat polyclonal antibody directed towards ZO-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:500 in PBS, v/v) or (2) with purified hamster anti-Occludin (Santa Cruz Biotechnology, 1:500 in PBS, w/v). Sections were washed with PBS and incubated with secondary antibody. A biotin-conjugated goat anti-rabbit IgG and avidin–biotin peroxidase complex (Vector Laboratories, Burlingame, CA, USA) detected the specific labeling. The software Optilab Graftek (Graftek, Mirmande, France) assessed densitometry of immunohistochemistry photographs.
Esposito E., Campolo M., Casili G., Lanza M., Franco D., Filippone A., Peritore A.F, & Cuzzocrea S. (2018). Protective Effects of Xyloglucan in Association with the Polysaccharide Gelose in an Experimental Model of Gastroenteritis and Urinary Tract Infections. International Journal of Molecular Sciences, 19(7), 1844.
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Publication 2018
anti-IgG Avidin Biotin Cloning Vectors Densitometry Formaldehyde Goat Hamsters Immunoglobulins Immunohistochemistry Infection Intestines Occludin Paraffin Embedding Peroxidase Rabbits Tissues
5
Antibody Sources and Cell Lines for Tight Junction Research
Cited 8 times
Rabbit anti-GFP pAb and rat anti-HA mAb (3F10) were purchased from Invitrogen and Roche, respectively. Rat anti-occludin mAb (MOC37) was raised and characterized as described previously (Saitou et al., 1998 (link)). Rat anti–E-cadherin mAb was provided by M. Takeichi (Center for Developmental Biology, Kobe, Japan).
Rabbit anti–mouse tricellulin (N450) was raised against a GST fusion protein with the NH2-terminal cytoplasmic domain in rabbits. Rat anti–mouse tricellulin mAbs (N54 and C96) specific for their NH2- and COOH-terminal cytoplasmic domains, respectively, were generated. Wistar rats were immunized with GST fusion proteins with the NH2-terminal 150–amino acid or COOH-terminal juxtamembranous 130–amino acid domain, and lymphocytes were fused with P3 myeloma cells to obtain hybridoma cells. All antibodies gave the same results in Western blotting and immunolabeling, but, as one pAb raised against the NH2-terminal cytoplasmic domain (N450) showed the highest titer among them, we used this pAb in our study.
Epithelial cells (mouse Eph4, CSG1, MTD1A, and dog MDCK cells) were cultured in DME supplemented with 10% FCS.
Ikenouchi J., Furuse M., Furuse K., Sasaki H., Tsukita S, & Tsukita S. (2005). Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. The Journal of Cell Biology, 171(6), 939-945.
Publication 2005
Amino Acids Antibodies CDH1 protein, rat Cells Cytoplasm Epithelial Cells Hybridomas Lymphocyte Madin Darby Canine Kidney Cells MARVEL Domain Containing 2 Protein Monoclonal Antibodies Multiple Myeloma Mus Occludin Oryctolagus cuniculus Proteins Rabbits Rats, Wistar Staphylococcal Protein A

Most recents protocols related to «Occludin»

1
Western Blot Analysis of Inflammatory and Tight Junction Proteins
Proteins were extracted from colon tissues with RIPA lysis buffer (Beyotime, Shanghai, China) containing phenylmethyl sulfonyl fluoride. A bicinchoninic acid protein assay kit (Beyotime) was used to measure the concentration of protein. Suitable quality protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Millipore, Billerica, MA, USA). The PVDF membranes were blocked with 8% skim milk at room temperature for 1 h and incubated with primary antibodies at 4 °C overnight. The corresponding secondary antibodies were used at room temperature for 1 h, and the protein signals were detected with a FluorChem FC3 system (ProteinSimple, California, USA) using an enhanced chemilusystem reagent (Thermo Fisher, Waltham, USA) according to the manufacturer’s instructions. The antibodies used in this study were as follows: TNFα (1:200, Santa Cruz Biotechnology, Texas, USA), IL-1β (1:1000, Cell Signaling Technology, Massachusetts, USA), IL6 (1:200, Santa Cruz Biotechnology), ZO-1 (1:1000, Invitrogen, California, USA), Occludin (1:1000, Invitrogen), P62 (1:10,000, Abcam, Cambridge, USA), LC3 (1:1000, Proteintech, Chicago, America), CB1 (1:1000, Proteintech), GAPDH (Abclonal, Wuhan, China), horseradish peroxidase (HRP)-linked goat anti-rabbit IgG, and HRP-linked goat anti-mouse IgG (1:4000, Antgene, Wuhan, China).
Yang J., Wang L., Mei M., Guo J., Yang X, & Liu S. (2023). Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis. Chinese Medicine, 18, 24.
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Publication 2023
anti-IgG Antibodies bicinchoninic acid Biological Assay Buffers Colon GAPDH protein, human Goat Horseradish Peroxidase Interleukin-1 beta Milk, Cow's Mus Occludin polyvinylidene fluoride Proteins Rabbits Radioimmunoprecipitation Assay SDS-PAGE sulfuryl fluoride Tissue, Membrane Tissues Tumor Necrosis Factor-alpha
2
Immunofluorescence Staining of Tight Junctions
Paraffin-embedded sections were dewaxed, hydrated, treated for antigen retrieval, and blocked with 10% donkey serum. Then, the primary antibodies were used for the incubation of the sections at 4 °C overnight. The next day, the slides were stained with relevant secondary antibodies at room temperature for 1 h. Nuclei were stained with DAPI (Servicebio, Wuhan, China). Finally, the sections were observed with a confocal microscope (Olympus, Tokyo, Japan) after sealing with an anti-fluorescence quencher. The antibodies used in this study were as follows: anti-ZO-1 (1:200, Genetex, Texas, America), anti-Occludin (1:200, Genetex), and Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:200, Antgene, Wuhan, China).
Yang J., Wang L., Mei M., Guo J., Yang X, & Liu S. (2023). Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis. Chinese Medicine, 18, 24.
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Publication 2023
alexa fluor 488 anti-IgG Antibodies Antigens Cell Nucleus DAPI Equus asinus Fluorescence Microscopy, Confocal Occludin Paraffin Rabbits Serum
3
Hippocampal Protein Expression Analysis
Western blotting was performed to determine the expression of occludin, alpha-2
spectrin, ERK1/2, phosphorylated ERK1/2 (pERK1/2), AKT, pAKT, and β-actin
(loading control) in the ipsilateral hippocampus of animals in different
experimental groups. The ipsilateral hippocampi were manually homogenized in
ice-cold lysis buffer, and respective supernatants were processed for
electrophoresis as we previously described.34 (link)–36 (link, link),39 (link) Total proteins (25 μg)
from each supernatant were separated by electrophoresis on 4-12%
SDS-polyacrylamide gels (Invitrogen, CA), then transferred to a nitrocellulose
membrane (Sigma, MO). Membranes were blocked with 5% non-fat milk in
Tris-buffered saline with .1% Tween-20 (TBST) and incubated overnight at 4°C in
rabbit polyclonal antibodies against occludin (1:2000; Abcam, MA), α-ii spectrin
(1:3000; Invitrogen/ThermoFisher Scientific, NY) ERK, 1/2, pERK1/2, AKT, pAKT
(1:1000; Cell Signaling Technology, MA) or a mouse anti-β-actin (1:4000; Sigma,
MO). Blots were washed in Tris-buffered saline with .1% Tween-20 and incubated
for 1 h at room temperature with corresponding HRP-conjugated secondary
antibodies (1:5000; Millipore, MA). Horseradish peroxidase-labeled proteins were
detected by enhanced chemiluminescence (ECL, Thermo Scientific, IL), and protein
bands were visualized using a digital blot scanner (LI-COR, NE).
Tchantchou F., Hsia R.C., Puche A, & Fiskum G. (2023). Hippocampal vulnerability to hyperhomocysteinemia worsens pathological outcomes of mild traumatic brain injury in rats. Journal of Central Nervous System Disease, 15, 11795735231160025.
Publication 2023
Actins Animals Antibodies Chemiluminescence Cold Temperature Electrophoresis Fingers Horseradish Peroxidase Milk, Cow's Mitogen-Activated Protein Kinase 3 Mus Occludin polyacrylamide gels Proteins Saline Solution Seahorses Spectrin Tissue, Membrane Tween 20
4
Assessing Blood-Brain Barrier Integrity
The sections made from rats selected using single-blinding were incubated with anti-occludin (Solarbio) and anti-ZO-1 (Solarbio) antibodies at 4°C overnight (Jin et al., 2021 (link)). After being washed with PBS, they were incubated together with the fluorescence-conjugated secondary antibody (Solarbio) at 25°Cfor for 1 h. In addition, the vascular endothelial cell markers CD31 were co-immunostaining with Zo-1 and occludin to observe the BBB integrity. The histopathological changes in the brain could be observed using a fluorescence microscope. The results were analyzed using ImageJ software.
Xu S., Li X., Li Y., Li X., Lv E., Zhang X., Shi Y, & Wang Y. (2023). Neuroprotective effect of Dl-3-n-butylphthalide against ischemia–reperfusion injury is mediated by ferroptosis regulation via the SLC7A11/GSH/GPX4 pathway and the attenuation of blood–brain barrier disruption. Frontiers in Aging Neuroscience, 15, 1028178.
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Publication 2023
Antibodies Brain Fluorescent Antibody Technique Microscopy, Fluorescence Occludin Rattus Vascular Endothelial Cells
5
Immunohistochemical Analysis of Mouse Colon
Mouse colon tissues were fixed using 4% paraformaldehyde and then divided into slices with a thickness of 40 μm using a vibrating knife (VT1200s, Leica, Wetzlar, Germany). After antigen retrieval with sodium citrate buffer, the slices were permeabilized with 0.1% Triton X-100 and blocked with the addition of 10% normal donkey serum for 1 h. Then slices were incubated overnight at 4 °C with primary antibodies rabbit anti-F4/F80 (ab6640, 1: 200), rabbit anti-iNOS (ab178945, 1: 500), mouse anti-Claudin (ab242370, 1: 500), rabbit anti-Occludin (ab216327, 1: 100), and rabbit anti-ZO-1 (ab221547, 1: 100). The slices were incubated with the secondary antibody (A32766/A-21203/A-21206/A32754, 1: 500, Thermo Fisher) coupled with Alexa Fluor 488/594 for 1 h. Subsequently, the slices were stained with 4’,6-Diamidino-2-Phenylindole (DAPI) for 10 min, and documented under a fluorescence microscope (Olympus).
Ning L., Ye N., Ye B., Miao Z., Cao T., Lu W., Xu D., Tan C., Xu Y, & Yan J. (2023). Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization. Cell Death Discovery, 9, 84.
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Publication 2023
alexa fluor 488 Antibodies Antigens Buffers Claudins Colon Equus asinus Immunoglobulins Mice, House Microscopy, Fluorescence NOS2A protein, human Occludin paraform Rabbits Serum Sodium Citrate Tissues Triton X-100

Top products related to «Occludin»

1
Occludin by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Canada, France
Occludin is a tight junction protein that plays a crucial role in the formation and maintenance of the blood-brain barrier. It is a transmembrane protein involved in cell-cell adhesion and the regulation of paracellular permeability. Occludin is commonly used as a marker for the integrity of tight junctions in various biological and biomedical applications.
2
Occludin by Abcam
Sourced in United States, United Kingdom, China
Occludin is a tight junction protein that plays a crucial role in maintaining the barrier function of epithelial and endothelial cells. It is an integral membrane protein that contributes to the formation and regulation of tight junctions, which are specialized cell-cell adhesion complexes responsible for controlling the movement of molecules across cell layers.
3
Anti occludin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany
Anti-occludin is a laboratory reagent used for the detection and analysis of the tight junction protein occludin in biological samples. It serves as a research tool for studying the structure and function of cell-cell junctions.
4
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
5
Pvdf membrane by Merck Group
Sourced in United States, Germany, China, United Kingdom, Morocco, Ireland, France, Italy, Japan, Canada, Spain, Switzerland, New Zealand, India, Hong Kong, Sao Tome and Principe, Sweden, Netherlands, Australia, Belgium, Austria
PVDF membranes are a type of laboratory equipment used for a variety of applications. They are made from polyvinylidene fluoride (PVDF), a durable and chemically resistant material. PVDF membranes are known for their high mechanical strength, thermal stability, and resistance to a wide range of chemicals. They are commonly used in various filtration, separation, and analysis processes in scientific and research settings.
6
Anti zo 1 by Thermo Fisher Scientific
Sourced in United States, Canada, United Kingdom, Germany
Anti-ZO-1 is a laboratory reagent used to detect the presence of the tight junction protein Zonula Occludens-1 (ZO-1) in cell samples. It is designed for use in immunohistochemistry, immunofluorescence, and related analytical techniques.
7
Ab216327 by Abcam
Sourced in United Kingdom, United States, China
Ab216327 is a reagent used in biochemical and molecular biology applications. It is a recombinant monoclonal antibody that recognizes a specific target. The core function of this product is to serve as a tool for detection, quantification, or purification of the target molecule in various experimental procedures.
8
Occludin by Proteintech
Sourced in United States, China
Occludin is a tight junction protein that plays a crucial role in maintaining the integrity of epithelial and endothelial cell barriers. It is a transmembrane protein that helps regulate the permeability of these cell layers by forming tight junctions between adjacent cells.
9
Claudin 5 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China
Claudin-5 is a tight junction protein that plays a critical role in the regulation of endothelial cell permeability. It is commonly used as a marker for monitoring the integrity of the blood-brain barrier and blood-retinal barrier.
10
Anti occludin by Abcam
Sourced in United States, United Kingdom, China
Anti-occludin is a primary antibody that binds to the occludin protein, a key component of tight junctions in epithelial and endothelial cells. It can be used to detect and analyze the expression and localization of occludin in various biological samples.

More about "Occludin"

Occludin is a critical transmembrane protein that plays a central role in the formation and regulation of tight junctions between epithelial and endothelial cells.
These cellular barriers are essential for maintaining proper organ function and homeostasis.
Occludin research focuses on understanding its structure, function, and potential therapeutic applications in various disease conditions, such as cancer, inflammation, and neurological disorders.
The tight junctions formed by Occludin are vital for controlling the permeability of these cellular barriers, which is crucial for organ health and overall bodily balance.
Anti-occludin antibodies can be used to study Occludin's role and localization within these junctions.
Additionally, TRIzol reagent and PVDF membranes are commonly used techniques and materials in Occludin research, while Anti-ZO-1 antibodies can help elucidate the interactions between Occludin and other tight junction proteins like Claudin-5.
PubCompare.ai's AI-driven platform can optimize Occludin research by identifying and evaluating the best protocols from literature, preprints, and patents, ensuring reproducible and accurate findings.
Leverage this intuitive platform, like product Ab216327, to locate the top Occludin research and products for your studies, and unlock new insights to advance your scientific discoveries.