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OPRS1 protein, human

The OPRS1 protein, also known as the Outer Membrane Protein RAS-like 1, is a protein involved in cellular signaling pathways.
It plays a role in regulating cellular processes such as proliferation, differentiation, and apoptosis.
The OPRS1 protein is found in the outer membrane of mitochondria and interacts with other cellular components to transduce signals.
Researchers can explore the OPRS1 protein using PubCompare.ai's AI-driven platform, which provides access to protocols from literature, preprints, and patents.
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Most cited protocols related to «OPRS1 protein, human»

1
Crystallization and Structure Determination of σ1 Receptor
Cited 22 times
To obtain phases, crystals of σ1 receptor bound to PD144418 were grown using a hanging-drop LCP methodology adapted from a previous report32 (link). In brief, this entailed dispensing cubic phase drops onto a plastic cover film (Art Robbins Instruments) and overlaying with precipitant solution as described above. This film was then inverted over a matched plate with identical crystallization solutions to the precipitant surrounding the lipid drop. The resulting crystals could be soaked and resealed, unlike conventional glass sandwich lipidic cubic phase plates. Crystals prepared in this way were soaked with tantalum bromide clusters for approximately 12 hours by adding crushed granules of tantalum clusters to the edge of the well. The crystals were harvested and data collected as described above, but at a wavelength of 1.2548 Å.
Initial phases were obtained in SHARP33 using single isomorphous replacement and anomalous scattering (SIRAS). Three transmembrane α-helices were identifiable in the initial map, suggesting three molecules in the asymmetric unit with an unusual solvent content of ~70%. Experimental phases were iteratively combined with model-derived phase to improve the electron density map through solvent flattening in SHARP. Model building was performed in Coot34 , and refinement was performed in phenix.refine35 . All three chains are highly similar in structure, with all-atom pairwise RMSD of cytosolic domains ranging from 0.22 Å to 0.26 Å, while the orientation of the transmembrane helix relative to the soluble domain varies among protomers.
Assignment of sequence register was straightforward and unambiguous due to the relatively high resolution, almost completely ordered structure, and high frequency bulky amino acid side chains (σ1 receptor is roughly 5% tryptophan). As a control for register assignment, the structure was built and register assigned in two independent ways. First it was manually built and register assigned by inspection of electron density. In parallel, sequence register was independently assigned automatically with phenix.autobuild, and results were confirmed to be identical throughout the entire polypeptide chain of each protomer. Representative composite omit map density is shown in Extended Data Figure 2. Ligands were manually placed into Fo−Fc difference maps (Extended Data Figure 7). In the case of PD144418 the electron density was clear, and ligand position and pose were unambiguous. For 4-IBP, the pose was unambiguous due to the high Fo−Fc peak resulting from the ligand iodine atom. Following refinement, structure quality was assessed using MolProbity36 , and figures were prepared in PyMOL37 and UCSF Chimera38 (link). All crystallographic data processing, refinement, and analysis software was compiled and supported by the SBGrid Consortium39 .
Schmidt H.R., Zheng S., Gurpinar E., Koehl A., Manglik A, & Kruse A.C. (2016). Crystal structure of the human σ1 receptor. Nature, 532(7600), 527-530.
Publication 2016
Amino Acids Crystallization Crystallography Cuboid Bone Cytoplasmic Granules Cytosol Dietary Fiber Electrons Helix (Snails) Iodine Ligands Lipids Microtubule-Associated Proteins OPRS1 protein, human PD 144418 Polypeptides Protomers Solvents Tantalum tantalum bromide Tryptophan
2
Molecular Docking of σ1 Receptor Ligand
Cited 8 times
Molecular docking into the σ1 receptor was performed in the manner of previous work55 using Glide 5.5 extra precision (XP) Maestro 11 Schrodinger release 2016–356 . PRE-084 was docked into either the structure of the σ1 receptor bound to (+)-pentazocine (PDB ID: 6DK1). Since the structure has three protomers in the asymmetric unit, only chain C was used for docking studies. Lipids, ions, and waters were removed prior to protein preparation, leaving only the protein and ligand. Hydrogen atoms were added, and the protein was further refined by assigning H-bonds and minimizing energy for the OPLS3 force field. The grid used for docking was centered on the location of the co-crystallized ligand, and was 20 Å in the x, y, and z dimensions. Poses were ranked by glide score.
Schmidt H.R., Betz R.M., Dror R.O, & Kruse A.C. (2018). Structural basis for σ1 receptor ligand recognition. Nature structural & molecular biology, 25(10), 981-987.
Publication 2018
Hydrogen Ions Ligands Lipids OPRS1 protein, human Pentazocine PRE 084 Proteins Protomers
3
Immunohistochemical Analysis of Sigma-1 Receptor in Retina
Cited 6 times
Following euthanasia of the mice, eye balls were enucleated immediately and dissected. The eyecups were fixed in 4% paraformaldehyde for 7 h, and then cryoprotected in 30% sucrose in PBS for another 14 h, all at 4 °C. Cryosections of 10 μm each were cut from the eyecups frozen in the optimum cutting temperature (O.C.T.) embedding medium (Sakura Finetek 4583, Sakura Finetek USA, Inc., Torrance, CA), and used for immunostaining following our method described previously42 (link) with minor modifications. Briefly, retinal sections were permeabilized with 1% Triton X-100 in PBS for 20 min, blocked with 10% normal goat serum (Cat#71–00–27; Kirkegaard & Perry Laboratories, Gaithersburg, MD) for 2 h at room temperature, and then incubated with purified rabbit anti-sigma-1 receptor antibody31 (link) (1/150 dilution) and mouse monoclonal anti-synaptophysin (Cat. #MAB368, 1/500 dilution; Chemicon, Los Angeles, CA) overnight at 4 °C. After rinsing the sections 3×, secondary antibodies (Alexa-488 conjugated goat-anti-rabbit and Alexa-594-conjugated goat-anti-mouse) at 2 μg/ml was applied at room temperature for 2 h. Sections were then rinsed 3×, counterstained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 min, and then mounted in the Prolong Gold mounting medium (Invitrogen, Carlsbad, CA) and cover-slipped. The slides were left in the dark overnight and then sealed using clear nail polish (Electron Microscopy Sciences, Hatfield, PA). Images were then taken with a Nikon A1R laser confocal microscope (Nikon, Tokyo, Japan) supplied with a green 488 nm Argon laser and a red 561 nm DPSS laser through an Apo60X VC oil-immersion objective with NIS elements software23 (link). Z-stacks were collected at 0.5 μm each, for a total thickness of 10 μm. Sequentially collected images were stacked together using the ImageJ program.
The porcine, bovine, monkey, and human retina sections were prepared similarly. The porcine and bovine eyeballs were obtained from the Black Earth Meats slaughter house in Cross Plain, WI, USA. The monkey retinal sections were from the Primate Research Center, University of Wisconsin, Madison, WI, USA. The human eyeball was procured from the Minnesota Lion Eye Bank.
Mavlyutov T.A., Epstein M, & Guo L.W. (2015). Subcellular Localization of the Sigma-1 Receptor in Retinal Neurons — an Electron Microscopy Study. Scientific Reports, 5, 10689.
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Publication 2015
Alexa594 anti-synaptophysin Antibodies Argon Ion Lasers Cattle Cryoultramicrotomy Electron Microscopy Euthanasia Eye Freezing Goat Gold Homo sapiens Laser Microscopy Meat Mice, House Monkeys Nails OPRS1 protein, human Panthera leo paraform Pigs Primates Rabbits Retina Serum Submersion Sucrose Technique, Dilution Triton X-100
4
Genetic Disorder Classes and Gene Intolerance
Cited 6 times
To determine the disorder classes that are most likely to be affected by mutations in intolerant genes, we rely on previously curated lists of OMIM genes categorised into the 22 disorder classes by Goh et al. 2007 as part of the human disease network diseasome mapping effort [26] (link). The disorder class annotations are published in Goh et al. (2007) “Supporting Information Table 1”. [http://www.pnas.org/content/suppl/2007/05/03/0701361104.DC1/01361Table1.pdf - last accessed 27th December 2012]. We filtered only for HGNC genes within the source list that were assigned an RVIS value. We summarized the RVIS within each of the 22 disorder classes (Table S2).
To compare RVIS values in an early versus late-onset genetic disorder context, we took epileptic encephalopathy (EE) genes from OMIM to represent “early-onset”: ARX (EIEE1 – OMIM# 308350), CDKL5 (EIEE2 – OMIM# 300672), SLC25A22 (EIEE3 – OMIM# 609304), STXBP1 (EIEE4 – OMIM# 612164), SPTAN1 (EIEE5 – OMIM# 613477), SCN1A (EIEE6 – OMIM# 607208), KCNQ2 (EIEE7 – OMIM# 613720), ARHGEF9 (EIEE8 – OMIM# 300607), PCDH19 (EIEE9 – OMIM# 300088), PNKP (EIEE10 – OMIM# 613402), SCN2A (EIEE11 – OMIM# 613721), PLCB1 (EIEE12 – OMIM# 613722), SCN8A (EIEE13 – OMIM# 614558), KCNT1 (EIEE14 – OMIM# 614959), MAPK10 (LGS EE – OMIM# 606369). Of these 16 EE genes, ARX was not assigned an RVIS score because it was insufficiently covered (less than 70% of gene) in the NHLBI-ESP (Methods). Of the remaining 15 genes, ST3GAL3, ARHGEF9, SLC25A22, PNKP, and PLCB1 lacked OMIM annotation for a dominant model. The genes considered for amyotrophic lateral sclerosis (ALS), a “late-onset” severe neuronal disorder, were similarly extracted from OMIM: SOD1 (ALS1 – OMIM# 105400), ALS2 (ALS2 – OMIM# 205100), SETX (ALS4 – OMIM# 602433), FUS (ALS6 – OMIM# 608030), VAPB (ALS8 – OMIM# 608627), ANG (ALS9 – OMIM# 611895), TARDBP (ALS10 – OMIM# 612069), FIG4 (ALS11 – OMIM# 612577), OPTN (ALS12 – OMIM# 613435), VCP (ALS14 – OMIM# 613954), UBQLN2 (ALS15 – OMIM# 300857), SIGMAR1 (ALS16 – OMIM# 614373), CHMP2B (ALS17 – OMIM# 614696), PFN1 (ALS18 – OMIM# 614808), C9orf72 (ALS – OMIM# 105550). Of the 15 ALS genes, ALS2 and SIGMAR1 lacked OMIM annotation for a dominant model. OMIM susceptibility genes “{” were not considered, and only genes with reported causal genetic variants were eligible.
Petrovski S., Wang Q., Heinzen E.L., Allen A.S, & Goldstein D.B. (2013). Genic Intolerance to Functional Variation and the Interpretation of Personal Genomes. PLoS Genetics, 9(8), e1003709.
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Publication 2013
Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis 1 Amyotrophic Lateral Sclerosis 2, Juvenile Amyotrophic Lateral Sclerosis 4, Juvenile Amyotrophic Lateral Sclerosis 8 Amyotrophic Lateral Sclerosis 9 Amyotrophic Lateral Sclerosis 11 CDKL5 Disorder Encephalopathies Epilepsy Epileptic Encephalopathy, Early Infantile, 3 Epileptic Encephalopathy, Early Infantile, 4 FIG4 protein, human Genes Genetic Diversity Homo sapiens Hyperekplexia and Epilepsy Infantile Severe Myoclonic Epilepsy Infantile Spasm Syndrome, X-Linked 1 Mutation Neurons OPRS1 protein, human Peptide Nucleic Acids protein TDP-43, human SETX protein, human Susceptibility, Disease
5
Antibody Generation and Characterization
Cited 6 times
Rabbit polyclonal antibody against STING was described previously4 (link). The antibody against STING-C was generated by immunizing rabbit with recombinant glutathione S-transferase (GST)–hSTING-C (amino acids 173–379) produced in E. coli. Rabbit polyclonal antibody against Sec5 was a gift from H. Horiuchi. Other antibodies were obtained from following sources: caspase-1 p10 (Santa Cruz Biotechnology), calreticulin (ab14234; Abcam), Sigma1 receptor (ab53852; Abcam), TBK1 (EP611Y, Abcam), COXIV (ab16056, Abcam), rabbit polyclonal HA (ab9110; Abcam), transferrin receptor (H68.4; Invitrogen), mouse monoclonal haemagglutinin (Sigma), Flag (M2; Sigma), IRF3 (ZM3; Zymed), TGN46 (ab16059; Abcam), giantin (ab24586; Abcam), EEA1 (no.2441; Cell Signaling), LAMP1 (NB120; Novus Biologicals) and Sec61β (Upstate). ELISA kits were obtained from following sources: murine IFNβ and IFNα (PBL), murine IL6 (R&D systems or Quansys Biosciences), murine IL1β and IFNγ (R&D systems), active NF-κB p65 (Active Motif), and murine RANTES (Quansys Biosciences).
Ishikawa H., Ma Z, & Barber G.N. (2009). STING regulates intracellular DNA-mediated, type I interferon-dependent innate immunity. Nature, 461(7265), 788-792.
Publication 2009
Amino Acids Antibodies Biological Factors Calreticulin Caspase 1 CCL5 protein, human Enzyme-Linked Immunosorbent Assay Escherichia coli Glutathione S-Transferase C Hemagglutinin Immunoglobulins Interferon-alpha Interferon Type II Interleukin-1 beta IRF3 protein, human lysosomal-associated membrane protein 1, human macrogolgin Mus Novus OPRS1 protein, human Rabbits TBK1 protein, human Transcription Factor RelA Transferrin Receptor

Most recents protocols related to «OPRS1 protein, human»

1
Antidepressant Use and Severity Outcomes

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Publication 2023
Antidepressive Agents Cardiac Arrest Fluoxetine OPRS1 protein, human Patients Selective Serotonin Reuptake Inhibitors SMPD1 protein, human SNRIs Vaccination
2
Generation of S1R Knockout ARPE-19 Cells
The human RPE cell line (ARPE-19) was purchased from American Type Culture Collection (CRL-2302, ATCC, Manassas, VA, USA). The generation of the S1R KO ARPE-19 cell line using a CRISPR/Cas9 genome-editing approach was described in our previous report [9 (link)]. In brief, the SIGMAR1-targeting single-guide RNA (sgRNA) sequence was cloned into lentiCRISPR v2. Packaged lentivirus was used to transduce ARPE-19 cells for 3 days. The cells were selected with 1 μg/mL of puromycin for one week, and serial dilution of the cells was performed for the selection of S1R KO single clones. The cells transduced with the empty vector served as the WT control.
Li J., Satyshur K.A., Guo L.W, & Ruoho A.E. (2023). Sphingoid Bases Regulate the Sigma-1 Receptor—Sphingosine and N,N’-Dimethylsphingosine Are Endogenous Agonists. International Journal of Molecular Sciences, 24(4), 3103.
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Publication 2023
Cell Lines Cells Clone Cells Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats Homo sapiens Lentivirus OPRS1 protein, human Puromycin RNA, Single Guide Technique, Dilution
3
Western Blot Analysis of Mitochondrial Proteins
Total protein was extracted and quantified as described [12 (link)]. The protein loading of each sample was 50 µg onto 4–12% NuPAGE™ Precast Gels. After electrophoresis, proteins were transferred to a nitrocellulose membrane in an iBlot Gel transfer device (Invitrogen, Carlsbad, CA, USA), then incubated with the primary antibodies at 4ºC overnight. The primary antibodies used in Western Blot analysis were as follow PCCB (1:1000, Santa Cruz Biotechnology, HERP (1:100, Enzo Life Science, Farmingdale, NY, USA), GRP78 (1:1000, Novus Biological, Centennial, CO, USA ), GRP75 (1:1000, Abcam, Cambridge, UK), SIG-1R (1:1000, Santa Cruz Biotechnology), MFN2 (1:1000, Abnova, Taipei, Taiwan), S6 (1:1000, Cell Signaling, Danvers, MA, USA), phosphorylated S6 (1:1000, Cell Signaling), catalase (1:1000, Abcam), BCL2 (1:500, Cell Signaling), caspase 3 (1:1000, Cell Signaling), LAMP1 (1:1000, Cell Signaling), ATG5 (1:500, Santa Cruz Biotechnology), p62 (1:2000, Novus Biologicals). The secondary antibodies were anti-mouse IgG HRP-linked (1:2000, Cell Signaling) and anti-rabbit IgG HRP-linked (1:5000, Cell Signaling). Antibody against GAPDH was used as a loading control (1:5000, Abcam). Enhanced chemiluminescence reagent (ECL, GE Healthcare, Chicago, IL, USA) was used for protein detection. Band intensity for each protein was quantified with BioRad GS-900 Densitometer (BioRad, Hercules, CA, USA) and ImageLab program (BioRad, Hercules, CA, USA).
Álvarez M., Ruiz-Sala P., Pérez B., Desviat L.R, & Richard E. (2023). Dysregulated Cell Homeostasis and miRNAs in Human iPSC-Derived Cardiomyocytes from a Propionic Acidemia Patient with Cardiomyopathy. International Journal of Molecular Sciences, 24(3), 2182.
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Publication 2023
anti-IgG Antibodies BCL2 protein, human Biological Factors Biopharmaceuticals Caspase 3 Catalase Chemiluminescence Electrophoresis GAPDH protein, human Glucose Regulated Protein 78 kDa GRP75 Immunoglobulins lysosomal-associated membrane protein 1, human Medical Devices mitofusin 2 protein, human Mus Nitrocellulose Novus OPRS1 protein, human Proteins Rabbits Tissue, Membrane Western Blot
4
Antibodies and Reagents for Membrane Protein Studies
The p97 (10736-1-AP; WB: 1:2000), UBXD8 (16251-1-AP; WB: 1:2000), FACL4 (22401-1-AP; WB: 1:2000), UBXD2 (21052-1-AP; WB: 1:2000), HRD1 (13473-1-AP; WB: 1:2000), Sec61β (51020-2-AP; WB: 1:2000), Calnexin (10427-2-AP; WB: 1:2000), UBXN1 (16135-1-AP; WB: 1:3000), SREBP1 (14088-1-AP; WB: 1:2000), SREBP2 (28212-1-AP; WB: 1:2000), FADS1 (10627-1-AP; WB: 1:2000), anti-GFP (66002-1-AP; WB: 1:2000), AMFR/GP78 (16675-1-AP; WB: 1:2000), GRP75 (14887-1-AP; WB: 1:2000), VAPB (14477-1-AP; WB: 1:2000), and SCD1 (23393-1-AP; WB: 1:2000) antibodies were from Proteintech Inc. The TIMM23 (H-8; sc514463; WB: 1:2000), TOMM20 (F-10; sc17764; WB: 1:2000; IF: 1:100), TOMM70 (A-8; sc390545; WB: 1:2000), pan-ubiquitin (P4D1; sc8017; WB: 1:2000), c-Myc (9E10; sc40; WB: 1:2000), β-Actin (AC-15; sc69879; WB: 1:2000), SIGMA1R (B-5; sc137075; WB: 1:2000), GAPDH (O411; sc47724; WB: 1:2000), and PCNA (PC10; sc56; WB: 1:2000) antibodies were obtained from Santa Cruz Biotechnologies. LC3B (D11; 3868S; WB: 1:1000), and BiP (C50B12; 3177T; WB: 1:2000) were from Cell Signaling Technologies. p97 (A300-589A; WB: 1:2000) was from Bethyl Laboratories. The following antibodies Histone-H3 (ab1791; Abcam; WB: 1:2000), UBXD7 (PA5-61972; Invitrogen; WB: 1:2000), anti-HA (16B12; MMS-101P, Covance; WB: 1:2500), anti-FLAG (M2; F3165 Sigma Aldrich; WB: 1:5000), were used for immunoblotting. HRP conjugated anti-rabbit (W401B; WB: 1:10,000) and anti-mouse (W402B; WB: 1:10,000) secondary antibodies were from Promega. Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (Catalog # A-11004; IF: 1:10,000), and Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Catalog # A-11001; IF: 1:10,000) were purchased from Thermofisher Scientific. CB-5083 was a gift from Cleave Biosciences and Bortezomib was from Selleckchem. Palmitic acid (100905) is from MP Biomedicals and Oleic acid (270290050) is from Acros Organics. MF438 (569406) is from Millipore Sigma. BODIPY 493/503 (item ono. 25892) is from Cayman chemical company. All siRNAs were purchased from Ambion (Thermo Fisher Scientific): UBXD8-0 (s23260), UBXD8-9 (s23259), UBXD7-7 (s24997), NPL4-0 (s31210), NPL4-9 (s31209), and UFD1-7 (s14637). UBXD2-1 (D-014184-03), UBXD2-2 (D-014184-04), HRD1-3 (D-007090-03), and HRD1-4 (D-007090-04) were purchased from GE Dharmacon. siControl (SIC001) was from Millipore Sigma. p97 siRNAs (2-HSS111263 and 3-HSS111264), SCD1 siRNA (5’-GAUAUGCUGUGGUGCUUAA-3’)63 (link), and SREBP1 siRNA (5’-AUCUCUGAAGGAUCUGGUG-3’)64 (link) were from Invitrogen (Thermo Fisher Scientific). p97 rescue constructs were previously published65 (link) and were resistant to siRNA # 2. UBXD8-C-HA/FLAG construct was previously published65 (link). The UBXD8 rescue constructs, including UBA* (17LLQF20 mutated to 17AAAA20), ΔUAS (deleted amino acids between 122-277), and UBX* (407FPR409 mutated to 407AAA409), were cloned using overlap PCR followed by Gibson assembly (NEB) cloning into pHAGE-C-HA/FLAG and were resistant to siRNA # 0. The SCD1-C-HA/FLAG WT and catalytic dead mutant (His160 His161 and His301 His302 mutated to Ala160 Ala161 and Ala301 Ala302) constructs were cloned using overlap PCR followed by Gateway cloning (Thermo Fisher Scientific) into pHAGE-C-HA/FLAG.
Ganji R., Paulo J.A., Xi Y., Kline I., Zhu J., Clemen C.S., Weihl C.C., Purdy J.G., Gygi S.P, & Raman M. (2023). The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition. Nature Communications, 14, 638.
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Publication 2023
4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene Actins alexa 568 alexa fluor 488 AMFR protein, human Amino Acids anti-IgG Antibodies Bacteriophages Bortezomib Caimans Calnexin Catalysis CB-5083 fatty acid desaturase 1, human GAPDH protein, human Goat GRP75 Histone H3 Immunoglobulins Mus Oleic Acid Oncogenes, myc OPRS1 protein, human Palmitic Acid Proliferating Cell Nuclear Antigen Promega Rabbits RNA, Small Interfering SREBF1 protein, human TOMM20 protein, human TOMM70 protein, human Ubiquitin UFD-1
5
Comprehensive Genetic Profiling of ALS Patients
A first cohort of 58 ALS patients was analyzed using a HaloPlex target enrichment system (Agilent Technologies, Santa Clara, CA, USA). A second cohort of 118 ALS patients (FLS and SLAS) was analyzed using a Twist target enrichment system (Twist Bioscience, CA, USA). The coding regions and intron–exon boundaries of the genes were analyzed. The following genes, known to be involved in ALS, were present in both designs: ALS2, ANG, CHCHD10, CHMP2B, DAO, DCTN1, DPYSL3, FIG4, FUS, GRN, MAPT, MATR3, NEFH, OPTN, PRPH, PSEN1, PSEN2, SETX, SIGMAR1, SOD1, SPG11, QSTM1, TAF15, TARDBP, TBK1, TREM2, TUBA4A, UBQLN2, VAPB and VCP. The FUS gene, listed as a causative gene for ALS, encodes an E3 enzyme of the ubiquitin pathway. Other ubiquitin pathway genes that have been studied were the following: in the first design (cohort 1) they were FBXO32, HECW1, MARCH5, RBX1, RNF19A, TRIM63, UBE2D2, UBED2D3, UHRF2; in the second design (cohort 2) they were HECW1, CCNF, UBQLN2. Libraries were sequenced using a MiSeq sequencer (Illumina, San Diego, CA, USA) with 150 bp paired-end sequencing (MiSeqReagent Kit V2; 300cycles) The sequences were analyzed using the bioinformatics pipeline of the ALS Center of the Hospital of Tours and the human reference genome UCSC hg19. Reads were aligned with the BWA algorithm (v.0.7.17), and variants were named using GATK tools (v.3.4) and annotated using the ANNOVAR software. Coverage was analyzed with samtools (v.1.8); variants were selected with minimum 30x coverage. Allelic frequencies in Exact databases and 1000 Genomes Projects were below 0.01% for all populations. Variants identified by NGS were validated by Sanger sequencing using a 3130xl genetic Analyzer (ThermoFisher) and classified according to ACMG [36 (link)].
Haouari S., Andres C.R., Lanznaster D., Marouillat S., Brulard C., Dangoumau A., Ung D., Veyrat-Durebex C., Laumonnier F., Blasco H., Couratier P., Corcia P, & Vourc’h P. (2023). Study of Ubiquitin Pathway Genes in a French Population with Amyotrophic Lateral Sclerosis: Focus on HECW1 Encoding the E3 Ligase NEDL1. International Journal of Molecular Sciences, 24(2), 1268.
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Publication 2023
Amyotrophic Lateral Sclerosis 2, Juvenile DPYSL3 protein, human Enzymes Exons FIG4 protein, human Genes Genome Genome, Human Introns MAPT protein, human NEFH protein, human OPRS1 protein, human Patients Population Group protein TDP-43, human PSEN1 protein, human PSEN2 protein, human RBX1 protein, human Reproduction SETX protein, human SLA protein, human Spastic paraplegia 11, autosomal recessive TAF15 protein, human TBK1 protein, human TREM2 protein, human UBE2D2 protein, human Ubiquitin

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Hiseq 2500 by Illumina
Sourced in United States, China, Germany, United Kingdom, Canada, Switzerland, Sweden, Japan, Australia, France, India, Hong Kong, Spain, Cameroon, Austria, Denmark, Italy, Singapore, Brazil, Finland, Norway, Netherlands, Belgium, Israel
The HiSeq 2500 is a high-throughput DNA sequencing system designed for a wide range of applications, including whole-genome sequencing, targeted sequencing, and transcriptome analysis. The system utilizes Illumina's proprietary sequencing-by-synthesis technology to generate high-quality sequencing data with speed and accuracy.
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Benzonase nuclease by Merck Group
Sourced in United States, Germany, Macao, Switzerland, United Kingdom, France
Benzonase nuclease is a recombinant endonuclease that catalyzes the hydrolytic cleavage of DNA and RNA. It is effective in removing nucleic acids from protein samples, cell extracts, and other biological preparations.

More about "OPRS1 protein, human"

The OPRS1 protein, also known as the Outer Membrane Protein RAS-like 1, is a critical component of cellular signaling pathways.
It plays a pivotal role in regulating essential cellular processes such as proliferation, differentiation, and apoptosis.
This mitochondrial outer membrane protein interacts with various cellular elements to transduce signals, making it a key player in cellular homeostasis.
Researchers can explore the OPRS1 protein using advanced tools like PubCompare.ai's AI-driven platform.
This cutting-edge technology provides access to a wealth of protocols from literature, preprints, and patents, allowing researchers to identify the most effective and reproducible techniques.
By leveraging intelligent comparisons, scientists can accelerate their research and improve the overall reproducibility of their findings.
The PubCompare.ai platform also integrates seamlessly with other powerful research tools, such as Lipofectamine 2000 for efficient transfection, TRIzol and TRIzol reagent for RNA extraction, Opti-MEM for cell culture optimization, and the Agilent 2100 Bioanalyzer for high-quality RNA analysis.
Additionally, researchers can utilize DMSO for cryopreservation, the HiSeq 3000 and HiSeq 2500 for advanced sequencing, and the 7500 Fast Real-Time PCR System for precise gene expression analysis.
By combining the insights gained from the OPRS1 protein description with the capabilities of PubCompare.ai and other cutting-edge research tools, scientists can unlock new discoveries, streamline their workflows, and drive progress in the field of cellular signaling and beyond.
Experince the power of AI-driven protocol discovery today with PubCompare.ai!