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Ovalbumin

Ovalbumin is a major storage protein found in egg white, comprising up to 54% of the total protein.
It is commonly used in research as a model protein for studying protein structure, folding, and function.
Ovalbumin has a molecular weight of approximately 45 kDa and contains 385 amino acids.
It can be readily purified from egg whites and is an important tool for immunological and biochemical studies.
Reserchers can optimize their ovalbumin research using PubCompare.ai to enhancing reproducibility and accuracy, locate the best protocols from literature, pre-pints, and patents, and streamline their research process for greater results.

Most cited protocols related to «Ovalbumin»

CD45.1 or Thy1.1 congenic OT-1 TCR transgenic naïve T cells were isolated using the CD8 untouched isolation kit (Miltenyi Biotech) plus biotin conjugated anti-CD44. Cells were transferred into C57/BL6 (Jackson Laboratories) mice 1 day prior to infection.
Recombinant Listeria monocytogenes strains were generated that stably express chicken Ovalbumin (AA134–387) containing either the native ligand SIINFEKL257–264 or the APL listed in Supp. Fig. 2. The designation of the APL indicates the substituted amino acid and the position within the SIINFEKL epitope. A previously described cassette24 (link) encoding for the expression of secreted Ova was manipulated by site-directed mutagenesis to insert the APL. The cassettes were cloned into a vector (pPL2) and stable Listeria recombinants were made as described25 (link). Mice were infected i.v. with 1000 CFU of log phase Listeria. For VSV-N4ova, mice were infected i.v. with 2×105 PFU.
Spleens were digested with Blendzyme 2 and DNAse 1 (both Roche) for 1 hour at 37°C and mashed through a 100 µm cell strainer (Becton Dickinson) to obtain single cell suspensions. Flow cytometry, intracellular cytokine staining (ICS), and CFSE labeling were performed using standard procedures. For ICS, the cells were first stimulated at 37°C for 30 min with peptide, then 7 µM Brefeldin was added and the incubation was continued for another 4.5 hours. For CCR7 staining, cells were incubated for 1 hour at 37°C and then stained on ice with CCL19-IgG fusion supernatant26 (link). To assess the level of non-specific binding of CCL19-IgG to OT-1, soluble CCL19 (R&D Systems) was added to control samples.
For immunofluorescent microscopy, spleens were fixed in 4% paraformaldehyde and kept in 30% sucrose over night. 10–20 µM sections, were cut and stained using standard procedures and analysed on a Zeiss 510 META confocal microscope.
Publication 2009
5-(6)-carboxyfluorescein diacetate succinimidyl ester Amino Acids Animals, Transgenic Biotin CCL19 protein, human CD44 protein, human Cells Chickens Cloning Vectors Cytokine Deoxyribonucleases Epitopes Flow Cytometry Immunofluorescence Microscopy Infection isolation Ligands Listeria Listeria monocytogenes Microscopy, Confocal Mus Mutagenesis, Site-Directed Ovalbumin paraform Peptides Protoplasm Strains Sucrose T-Lymphocyte
The intravital imaging preparation used in this study is similar to previously described methods11 (link),15 (link) with the following differences: imaging is performed with the tissue within the peritoneal cavity, fecal material is not scraped from the mucosal surface and in some experiments atropine (1 mg/kg) was injected subcutaneously to dampen peristaltic movement of the small intestine. At this dose atropine, did not affect the formation of TEDs or GAPs. Model fluorescent antigens, dextran (2–5mg), ovalbumin (2mg), BSA, (2mg) and FluoSpheres (1ml undiluted) (all from Invtirogen, Carlsbad, CA) were injected into the intestinal lumen ~2hrs minutes prior to imaging. Human resection specimens were incubated in 10ug/ml of dextran at room temperature for 1hr prior to imaging.
Publication 2012
Antigens Atropine Defecation Dextran Feces Homo sapiens Intestines Mucous Membrane Ovalbumin Peristalsis Peritoneal Cavity Tissues
PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS), 2% calf serum, 1 mM EDTA) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen, 47-0037-41), anti-CD8-APC-eFluro 780 (Invitrogen, 47-0086-42), anti-CD16-APC-eFluro 780 (Invitrogen, 47-0168-41), anti-CD14-APC-eFluro 780 (Invitrogen, 47-0149-42), as well as Zombie NIR (BioLegend, 423105), and fluorophore-labeled RBD and Ovalbumin for 30 minutes on ice32 (link). Single CD3CD8CD16CD20+OvaRBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson) for acquisition and FlowJo for analysis. The sorted cells were frozen on dry ice, and then stored at −80°C or immediately used for subsequent RNA reverse transcription. Although cells were not stained for IgG expression, they are memory B cells based on the fact that they are CD20+ (a marker absent in plasmablasts) and they express IgG (since antibodies were amplified from these cells using IgG-specific primers).
Publication 2020
Alexa Fluor 647 Anti-Antibodies Antibodies B-Lymphocytes Cells Cell Separation Dry Ice Edetic Acid Freezing Homo sapiens inhibitors Memory B Cells Muromonab-CD3 NRG1 protein, human Oligonucleotide Primers Ovalbumin Phosphates Promega Reverse Transcription Ribonucleases Saline Solution Serum Technique, Dilution
The dorsal skin of 6–8 week old female mice was shaven and stripped using a transparent bio-occlusive dressing (Tegaderm®; 3M). 108 CFU of S. aureus strains were placed on a patch of sterile gauze and attached to the shaved skin with another transparent bio-occlusive dressing (Tegaderm®; 3M). Each mouse was exposed to S. aureus for 1 week through the patch. After a 2-week interval, each mouse was challenged once with 100 µg ovalbumin epicutaneously for 1 week and the animals were sacrificed for analyses.
Publication 2013
Animals Mice, House Ovalbumin Skin Sterility, Reproductive Strains Woman
Initially, 2.5 × 109 induced yeast for the first round and 1.4 – 5 × 107 induced yeast for subsequent rounds were washed and resuspended in buffer (20 mM HEPES pH 7.5, 150 mM sodium chloride, 0.1% (w/v) ovalbumin, 1 mM EDTA) and then incubated with anti-Alexa Fluor 647 or anti-FITC microbeads (Miltenyi) at 4 °C for 30 min. Each round of MACS selection began with a pre-clear step which involved passing the yeast, through an LD column (Miltenyi) to remove yeast expressing nanobodies that bound nonspecifically to magnetic beads. After pre-clearing, human serum albumin (HSA) binding nanobodies were enriched over four rounds of MACS selection by staining the yeast alternately with Alexa Fluor 647 or FITC labeled HSA (Sigma) and microbeads, then passing them through an LS column (Miltenyi). During these four selection rounds, yeast were stained with successively lower concentrations of HSA: 1 μM, 250 nM, 75 nM,and 15 nM, in order to enrich for binders with higher affinities. After MACS selection, yeast were plated as single colonies which were picked and grown as clonal populations in a 96 well plate. Following galactose induction of nanobodies, yeast were stained with Alexa Fluor 488 labeled HSA and analyzed by flow cytometry with an Accuri C6 (BD Biosciences) to screen for nanobody binders.
Publication 2018
alexa fluor 488 Alexa Fluor 647 Buffers Clone Cells Edetic Acid Flow Cytometry Fluorescein-5-isothiocyanate Galactose HEPES Microspheres Ovalbumin Population Group Serum Albumin, Human Sodium Chloride VHH Immunoglobulin Fragments Yeast, Dried

Most recents protocols related to «Ovalbumin»

Mice were immunized in bilateral foot pads with 100 μl of a mixture of 100 μg papain (Sigma-Aldrich) and 100 μg 4-hydroxy-3-nitrophenylacetyl conjugated to ovalbumin (NP-ova; Sigma-Aldrich). After 5 d, inguinal and popliteal lymph nodes were harvested. Lymphocytes were prepared for calcium flux analysis as described below.
Publication 2023
Calcium Foot Groin Lymphocyte Mus Nodes, Lymph Ovalbumin Papain

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Publication 2023
Animals Anti-Antibodies Antibodies, Anti-Idiotypic Antibody Formation Antigens BLOOD Colostrum Enzyme-Linked Immunosorbent Assay IgG2A Milk neuro-oncological ventral antigen 2, human Ovalbumin Pharmaceutical Adjuvants Pregnancy SARS-CoV-2 Serum Sheep Vaccination

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Publication 2023
Amino Acids Amino Acid Sequence Antigens Base Sequence Consensus Sequence Densitometry Dietary Supplements Dimerization DNA, Complementary Enzyme-Linked Immunosorbent Assay Granulocyte-Macrophage Colony-Stimulating Factor IgG2 IgG2A Immunization Mutation Ovalbumin Protein C Protein Dimerization Proteins Rabbits SARS-CoV-2 SDS-PAGE Sheep Signal Peptides

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Publication 2023
ACE2 protein, human Angiotensin Converting Enzyme 2 Antigens Enzyme-Linked Immunosorbent Assay Milk, Cow's M protein, multiple myeloma Ovalbumin Powder prisma Proteins Technique, Dilution Tweens

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Publication 2023
Escherichia coli Fluorescein hen egg lysozyme Inulin Isothiocyanates Oils, Volatile Origanum vulgare Ovalbumin Staphylococcus aureus

Top products related to «Ovalbumin»

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The OVA is a laboratory equipment product designed for the detection and analysis of eggs or ova. It provides a reliable and standardized method for sample preparation and observation. The core function of the OVA is to facilitate the identification and quantification of eggs or ova in various samples.
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Aluminum hydroxide is a chemical compound with the formula Al(OH)3. It is a white, odorless, and tasteless powder that is insoluble in water. Aluminum hydroxide is primarily used as a food additive, antacid, and in the production of other aluminum compounds.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Imject Alum is a laboratory product used as an adjuvant. It is designed to enhance the immune response to administered antigens.
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Ovalbumin is a protein derived from egg white. It is commonly used as a reagent in various laboratory applications, including protein assays, electrophoresis, and immunology experiments.
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The Superdex 200 10/300 GL column is a size exclusion chromatography column designed for the purification and analysis of a wide range of biomolecules, including proteins, peptides, and other macromolecules. It features a prepacked, ready-to-use format with a bed volume of 24 mL and a separation range of 10,000 to 600,000 Da.
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The Gel Filtration Standard is a laboratory instrument used for the separation and purification of molecules based on their size and shape. It operates on the principle of size exclusion chromatography, allowing the separation of compounds within a sample mixture.
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DQ-ovalbumin is a fluorescently labeled protein used as a substrate for protease activity assays. It is derived from ovalbumin, a protein found in egg white. The fluorescent label allows for the detection and quantification of protease activity in biological samples.
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The OVA (grade V) is a lab equipment product from Merck Group. It is designed for laboratory use and serves a core function in the research and analysis processes. Due to the technical nature of this product, a detailed description while maintaining an unbiased and factual approach cannot be provided. Therefore, a more comprehensive description is not available.

More about "Ovalbumin"

Ovalbumin is a major storage protein found in egg whites, comprising up to 54% of the total protein content.
It is a popular model protein used in research to study protein structure, folding, and function.
Ovalbumin has a molecular weight of approximately 45 kilodaltons (kDa) and contains 385 amino acids.
It can be readily purified from egg whites and is an important tool for immunological and biochemical studies.
Researchers can optimize their ovalbumin research using PubCompare.ai to enhance reproducibility and accuracy.
This AI-driven platform helps researchers locate the best protocols from literature, preprints, and patents, allowing them to identify the most reliable and effective methods.
By streamlining the research process, PubCompare.ai can help researchers achieve greater results.
Ovalbumin is commonly used in research alongside other proteins like bovine serum albumin (BSA) and aluminum hydroxide (Alum).
BSA is another commonly used model protein, often employed as a blocking agent or standard in various assays.
Alum, on the other hand, is a common adjuvant used in vaccine formulations, including those that incorporate ovalbumin.
Fetal bovine serum (FBS) is another important component in cell culture and research, as it provides essential nutrients and growth factors.
Imject Alum is a specific form of aluminum hydroxide adjuvant that is frequently used in ovalbumin-based research and immunization studies.
When working with ovalbumin, researchers may use techniques like gel filtration chromatography, where a Superdex 200 10/300 GL column can be used to purify and separate the protein.
Gel filtration standards, such as the Gel Filtration Standard, can help in the calibration and analysis of these chromatographic separations.
Additionally, researchers may utilize modified forms of ovalbumin, such as DQ-ovalbumin, which is a fluorescently labeled version of the protein that can be used to track and visualize its uptake and processing in cellular studies.