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Oxidase, Glucose

Oxidase and Glucose are important biomolecules involved in various metabolic processes.
Oxidase catalyzes the oxidation of substrates, while Glucose is a primary source of energy for many cells.
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The platform helps locate accurate and reproducible protocols from literature, preprints, and patents, and provides AI-powered comparisons to identify the best methods and products for experiments.
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Most cited protocols related to «Oxidase, Glucose»

1
Longitudinal Health Examination Cohort
Cited 61 times
Data were extracted from a computerised database established by the Rich Healthcare Group in China, which includes all medical records for participants who received a health check from 2010 to 2016. The present analysis initially included all study participants who were at least 20 years old with at least two visits between 2010 and 2016 (n=685 277). Participants were excluded at baseline if they had no available weight and height measurements (n=103 946), no available information on gender (n=1), extreme BMI values (<15 kg/m2 or >55 kg/m2) (n=152) or no available fasting plasma glucose value (n=31 370). We further excluded participants with visit intervals less than 2 years (n=324 233), participants diagnosed with diabetes at baseline (2997 participants diagnosed by self-report and 4115 diagnosed by a fasting plasma glucose ≥7.0 mmol/L), and participants with undefined diabetes status at follow-up (n=6630). Finally, a total of 211 833 participants (116 123 male and 95 710 female) were included in the analysis. Cohort entry was defined as the date of the initial visit. Compared with individuals excluded from the present analyses, those included in the analyses were with similar age (42.1 vs 41.9 years old) and similar BMI (23.2 vs 23.3 kg/m2), and with a relatively higher proportion of males (54.8% vs 52.1%).
In each visit to the health check centre, participants were requested to complete a detailed questionnaire assessing demographic, lifestyle, medical history and family history of chronic disease. Height, weight and blood pressure were measured by trained staff. Body weight was measured in light clothing with no shoes to the nearest 0.1 kg. Height was measured to the nearest 0.1 cm. BMI was derived from weight in kilograms divided by height in metres squared. Blood pressure was measured by standard mercury sphygmomanometers.
Fasting venous blood samples were collected after at least a 10 hours fast at each visit. Serum triglyceride (TG), total cholesterol, low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol were measured on an autoanalyzer (Beckman 5800). Plasma glucose levels were measured by the glucose oxidase method on an autoanalyzer (Beckman 5800).
Chen Y., Zhang X.P., Yuan J., Cai B., Wang X.L., Wu X.L., Zhang Y.H., Zhang X.Y., Yin T., Zhu X.H., Gu Y.J., Cui S.W., Lu Z.Q, & Li X.Y. (2018). Association of body mass index and age with incident diabetes in Chinese adults: a population-based cohort study. BMJ Open, 8(9), e021768.
Publication 2018
Blood Pressure Cholesterol Cholesterol, beta-Lipoprotein Diabetes Mellitus Disease, Chronic Gender Glucose High Density Lipoprotein Cholesterol Light Males Mercury Oxidase, Glucose Plasma Serum Sphygmomanometers Triglycerides Veins Woman
2
Super-resolution Imaging of Fixed Cells
Cited 47 times
BS-C-1 cells (ATCC) were fixed, immunostained with rabbit anti-Tom20 (Santa Cruz Biotech) and/or mouse anti-β-tubulin (TUB2.1, Cytoskeleton) (See Supplementary Methods online for the detailed immunostaining procedure). The stained cells were imaged in PBS with the addition of 100 mM mercaptoethylamine at pH 8.5, 5% glucose (w/v) and oxygen scavenging enzymes (0.5 mg/mL glucose oxidase (Sigma-Aldrich), and 40 μg/mL catalase (Roche Applied Science)), unless otherwise mentioned. This above imaging buffer has a refractive index of 1.34. Media with higher refractive index (1.45) based on 80% (v/v) glycerol and 5% (w/v) glucose, or 60% (w/w) sucrose solution and 5% (w/w) glucose, were used in some experiments, both with the same amount of mercaptoethylamine and oxygen scavenging enzymes as described above. The slight mismatch between the medium refractive index and coverglass is needed for focus locking during imaging. Although a high concentration of mercaptoethylamine and oxygen scavenging system were used here for fixed cell imaging, the cyanine dyes also switch in buffers with lower concentrations of thiol and oxygen scavenger system compatible with live cell imaging12 (link).
Data acquisition was performed on a fluorescence microscope as described in the Supplementary Methods online. Specifically for 3D imaging, a cylindrical lens with a focal length of 1 m was inserted into the imaging optical path for 3D localization8 (link). To stabilize the microscope focusing during data acquisition, the reflected excitation laser from the coverglass-medium interface was directed to a quadrant photodiode. The position read out of the quadrant photodiode, which was sensitive to the distance between the coverglass and the objective focal plane, was used to provide feedback to a piezo objective positioner (Nano-F100, MadCity Labs), allowing compensation for the focus drift. The residual drift, < 40 nm (Supplementary Fig. 7 online), was corrected during data analysis. For whole cell imaging in an aqueous medium, the objective positioner was stepped in 300 nm intervals, which corresponds to a focal plane displacement of 216 nm after correcting for the refractive index mismatch at the glass-medium interface. Molecules within 270 nm below the focal plane were accepted for image reconstruction. Whole cell images were obtained from 9 partially overlapped z-slices. For imaging in media with a refractive index of 1.45, the positioner was stepped in 650 nm intervals, corresponding to an actual focal plane displacement of 580 nm. Molecules within 360 nm above and below the focal plane are accepted and whole cell images were obtained from 4 partially overlapped z-slices.
For single color imaging, the A405-Cy5 labeled sample was continuously illuminated with a 657 nm imaging laser (~30 mW). A low intensity 405 nm laser was used to activate the probes, with intensity adjusted such that only an optically resolved subset of the probes were activated at any given time. In certain cases, the 405 nm laser can be omitted because the 657 nm laser can also activate Cy5, albeit at a low rate. Emission from the fluorophores were recorded by the camera at a frame rate of 20 Hz. 3D localization of individual molecules was performed as described previously8 (link) and described in the Supplementary Methods online. Multicolor imaging was performed by illuminating the sample repetitively with each frame of an activation laser followed by 3 frames of the 657 nm imaging laser. An alternating sequence of two activation lasers was used for two-color imaging. The 405 nm, 460 nm and 532 nm lasers were used to activated A405-Cy5, A488-Cy5, and A555-Cy5, respectively. Subtraction of crosstalk between different color channels were performed during data analysis as described in the Supplementary Methods online.
Huang B., Jones S.A., Brandenburg B, & Zhuang X. (2008). Whole cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nature methods, 5(12), 1047-1052.
Publication 2008
A-A-1 antibiotic Buffers Catalase Cells Cross Reactions Cysteamine Cytoskeleton Dyes Enzymes Gas Scavengers Glucose Glycerin Lens, Crystalline Microscopy Microscopy, Fluorescence Mus Oxidase, Glucose Oxygen Rabbits Reading Frames Sucrose Sulfhydryl Compounds Tubulin
3
Super-resolution Imaging of Live and Fixed Cells
Cited 29 times
Super-resolution imaging experiments were performed on live samples (Fig. 2j, Supplementary Fig. 2c, Supplementary Fig. 4d, Supplementary Fig. 4g) and fixed cells (Fig. 1i, Fig. 2b, Supplementary Fig. 2a, Supplementary Fig. 4a). For live-cell dSTORM imaging the cells were labeled, washed, and imaged directly in DMEM–FBS. For fixed cell preparations, cells were labeled, washed, and fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS buffer (pH = 7.5). The cells were imaged in a sealed cell chamber (Life Technologies) containing nitrogen-degassed redox buffer consisting of PBS supplemented with 50 mM mercaptoethylamine (Sigma–Aldrich), 10% w/v glucose, 0.5 mg/mL glucose oxidase (Sigma–Aldrich), and 28400 U/mL catalase (Sigma–Aldrich). Before imaging, JF549 could be efficiently “shelved” in a dark state upon illumination with 2 kW·cm−2 of excitation light (561 nm), and then activated back to a fluorescent state by blue light (405 nm) with low intensity (~20·W cm−2). JF646 fluorophores were converted into a predominately dark state using continuous illumination of 637 nm excitation light at 14 kW·cm−2, after which individual rapidly blinking molecules of JF646 fluorophores were observed. These experiments were conducted on the two wide-field microscope systems described above: the Nikon Eclipse Ti epifluorescence microscope (Fig. 1i, Fig. 2j, Supplementary Fig. 2a, Supplementary Fig. 2c, Supplementary Fig. 4g), and the custom-built three-camera microscope with an ASI RAMM frame (Fig. 2b, Supplementary Fig. 4a, Supplementary Fig. 4d).
Grimm J.B., English B.P., Chen J., Slaughter J.P., Zhang Z., Revyakin A., Patel R., Macklin J.J., Normanno D., Singer R.H., Lionnet T, & Lavis L.D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature methods, 12(3), 244-250.
Publication 2014
Buffers Catalase Cells Cysteamine Electron Microscopy Glucose Light Microscopy Nitrogen Oxidase, Glucose Oxidation-Reduction paraform Reading Frames
4
Super-resolution Imaging of Live and Fixed Cells
Cited 27 times
Super-resolution imaging experiments were performed on live samples (Fig. 2j, Supplementary Fig. 2c, Supplementary Fig. 4d, Supplementary Fig. 4g) and fixed cells (Fig. 1i, Fig. 2b, Supplementary Fig. 2a, Supplementary Fig. 4a). For live-cell dSTORM imaging the cells were labeled, washed, and imaged directly in DMEM–FBS. For fixed cell preparations, cells were labeled, washed, and fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS buffer (pH = 7.5). The cells were imaged in a sealed cell chamber (Life Technologies) containing nitrogen-degassed redox buffer consisting of PBS supplemented with 50 mM mercaptoethylamine (Sigma–Aldrich), 10% w/v glucose, 0.5 mg/mL glucose oxidase (Sigma–Aldrich), and 28400 U/mL catalase (Sigma–Aldrich). Before imaging, JF549 could be efficiently “shelved” in a dark state upon illumination with 2 kW·cm−2 of excitation light (561 nm), and then activated back to a fluorescent state by blue light (405 nm) with low intensity (~20·W cm−2). JF646 fluorophores were converted into a predominately dark state using continuous illumination of 637 nm excitation light at 14 kW·cm−2, after which individual rapidly blinking molecules of JF646 fluorophores were observed. These experiments were conducted on the two wide-field microscope systems described above: the Nikon Eclipse Ti epifluorescence microscope (Fig. 1i, Fig. 2j, Supplementary Fig. 2a, Supplementary Fig. 2c, Supplementary Fig. 4g), and the custom-built three-camera microscope with an ASI RAMM frame (Fig. 2b, Supplementary Fig. 4a, Supplementary Fig. 4d).
Grimm J.B., English B.P., Chen J., Slaughter J.P., Zhang Z., Revyakin A., Patel R., Macklin J.J., Normanno D., Singer R.H., Lionnet T, & Lavis L.D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature methods, 12(3), 244-250.
Publication 2014
Buffers Catalase Cells Cysteamine Electron Microscopy Glucose Light Microscopy Nitrogen Oxidase, Glucose Oxidation-Reduction paraform Reading Frames
5
Longitudinal Glucose and Insulin Metabolism
Cited 20 times

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Publication 2009
Blood Glucose Diabetes Mellitus Diagnosis Enzyme-Linked Immunosorbent Assay Fluorides Glucose Homo sapiens Insulin Insulin Sensitivity Natural Springs Oral Glucose Tolerance Test Oxidase, Glucose Pharmaceutical Preparations Physicians Physiology, Cell Plasma Radioimmunoassay Serum Veins

Most recents protocols related to «Oxidase, Glucose»

1
Preparation and Application of Double-Cycled Microtubule Seeds
Double-cycled MT seeds were prepared by combining TRITC-labeled (49%), biotinylated (18%), and unlabeled tubulin (33%; Cytoskeleton) reconstituted in MRB80 (80 mM K-Pipes, 1 mM EGTA, 4 mM MgCl2; pH 6.80 with KOH) to a final concentration of 20 µM with 1 mM GMPCPP (Jena Bioscience) on ice. The mixture was incubated at 35°C for 30 min to allow MTs to polymerize. Seeds were pelleted by centrifugation in an airfuge (Beckman coulter) at 20 psi for 5 min, resuspended in MRB80, and depolymerized on ice for 25 min. The tubulin was then repolymerized upon the addition of fresh GMPCPP by incubating at 35°C for 30 min. These seeds were pelleted by centrifugation in an airfuge at 20 psi for 5 min, resuspended and diluted sixfold in MRB80 supplemented with 10% [vol/vol] glycerol, aliquoted, flash-frozen in liquid nitrogen, and stored at −80°C until use.
To prepare the chambers, a clean glass coverslip was plasma-treated and fixed to a clean glass slide using strips of double-sided tape to create two parallel chambers of ∼10 µl. The surface was blocked and functionalized by incubating with a mix of 95% PLL-g-PEG and 5% PLL-g-PEG-biotin (0.1 mg/ml in 10 mM Hepes, pH 7.40; SuSoS) for 10 min. After washing with MRB80 supplemented with 40% [vol/vol] glycerol (MRB80-gly40), NeutrAvidin was introduced and incubated for 10 min. After washing, 50-fold diluted GMPCPP seeds were introduced and incubated for 5 min before washing once more and then incubating with Κ-casein for >3 min.
All reaction mixtures (MT mix, expansion mix, rigor mix, washout mix) were prepared at double the volume for the paired compacted/expanded lattice samples and split into two equal parts prior to the addition of DMSO (compacted control) or 20 µM Taxol (expanded). Reagents were added to MRB80-gly40 such that the effective glycerol concentration in the MT mix was 20% and in the other mixes was ∼27%. All mixes contained 0.1% [wt/vol] methylcellulose, 0.5 mg/ml K-casein, 50 mM glucose, 0.2 mg/ml catalase, 0.5 mg/ml glucose oxidase, and 10 mM DTT. The MT mix additionally contained 1 mM GTP, 10.8 µM porcine tubulin (Cytoskeleton), and 0.6 µM TRITC-labeled porcine tubulin (Cytoskeleton). The expansion mix additionally contained 50 mM KCl and 20 µM Taxol (or the equivalent dilution of DMSO). The rigor mix additionally contained 50 mM KCl, 20 µM Taxol (or the equivalent dilution of DMSO), 2 mM ATP, and 15.2 pM StableMARK. The washout mixture additionally contained 50 mM KCl, 20 µM Taxol (or the equivalent dilution of DMSO), and 2 mM ATP. After preparation, these mixtures were spun in an airfuge at 20 psi for 5 min, transferred to clean tubes, and kept on ice until use.
Samples were then moved to the TIRF microscope equipped with a stage-top incubator to maintain them at a constant temperature of 30°C. MTs were grown by flowing in two chamber volumes (ChV) of the MT mix and letting it incubate for 15 min. Subsequently, the chambers were flushed with five ChV MRB80-gly40. Next, the lattices were (mock) expanded by adding two ChV expansion mix (or DMSO equivalent) and incubating for 10 min. Next, two ChV rigor mix was added and incubated for 90 s. Finally, four ChV washout mix was added before imaging. For imaging, the following sequence was used: 2 × Taxol, 4 × DMSO, 4 × Taxol, 4 × DMSO, and either 2 × Taxol or 4 × Taxol and 2 × DMSO (8 or 10 images/condition/assay), and images were taken at similar heights within the channels.
Jansen K.I., Iwanski M.K., Burute M, & Kapitein L.C. (2023). A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. The Journal of Cell Biology, 222(5), e202106105.
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Publication 2023
5'-guanylylmethylenebisphosphonate Biological Assay Biotin Caseins Catalase Centrifugation Cytoskeleton Egtazic Acid Freezing Glucose Glycerin HEPES K-Casein Magnesium Chloride Methylcellulose Microscopy Muscle Rigidity neutravidin Nitrogen Oxidase, Glucose Pigs piperazine-N,N'-bis(2-ethanesulfonic acid) Plant Embryos Plasma polylysine-graft-(poly(ethylene glycol)) Sulfoxide, Dimethyl Taxol Technique, Dilution tetramethylrhodamine isothiocyanate Tubulin
2
Fasting Biomarker Measurement Protocol
After maintaining a fasting state for 12 hours, blood samples were obtained from each participant to measure plasma glucose, HbA1C, creatinine, and lipid profiles. Serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol (LDL-C) were determined using the dry, multilayer analytical slide method in the Fuji Dri-Chem 3000 analyzer (Fuji Photo Film Corporation, Tokyo, Japan). The levels of HbA1C were evaluated by ion-exchange high-pressure liquid chromatography method (BIO-RAD VARIANT II, Los Angeles, CA). Plasma glucose concentrations were determined by the glucose oxidase method on a Beckman Glucose Analyzer II (Beckman Instruments, Fullerton, CA).
Liu J.S., Su S.C., Kuo F.C., Li P.F., Huang C.L., Ho L.J., Chen K.C., Liu Y.C., Lin C.P., Cheng A.C., Lee C.H., Lin F.H., Hung Y.J., Liu H.Y., Lu C.H, & Hsieh C.H. (2023). The efficacy and safety of combined GLP-1RA and basal insulin therapy among inadequately controlled T2D with premixed insulin therapy. Medicine, 102(10), e33167.
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Publication 2023
BLOOD Cholesterol Cholesterol, beta-Lipoprotein Creatinine Glucose High-Performance Liquid Chromatographies Ion Exchange Lipids Oxidase, Glucose Plasma Serum Triglycerides
3
Synthesis and Evaluation of Multifunctional Nanoparticles
3,3′,5,5′-Tetramethylbenzidine (TMB, 99%, CAS 54827-17-7) was purchased from Alfa Company. β-d-Glucose (β-d-Glu, 99%, CAS 28905-12-6) was purchased from TCI Company. Phosphate-buffered saline (PBS), trypsin (1 : 250, CAS 9002-07-7), Dulbecco’s modified eagle medium (DMEM), high-glucose DMEM (4500 mg L−1) and fetal bovine serum albumin (FBSA, 98%, CAS 9048-46-8) were purchased from Gibco, USA. HeLa cells were purchased from Beyotime Biotechnology Co. Ltd and Cell Counting Kit-8 (CCK8) was obtained from Beyotime Biotechnology Co. Ltd. Calcein acetoxymethyl ester (Calcein-AM, 90%, CAS 148504-34-1)/Propidium iodide (PI, 95%, CAS 25535-16-4) staining reagents were purchased from Yeasen Biotechnology (Shanghai) Co. Ltd. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, 97%, CAS 3317-61-1) was bought from J&K Scientific Company. Ferric chloride hexahydrate (FeCl3·6H2O, 98%, CAS 7705-08-0), ferrous chloride tetrahydrate (FeCl2·4H2O, 99%, CAS 13478-10-9), oleic acid (OA, 90%, CAS 112-80-1), polyoxyethylene oxypropylene ether block copolymer (Pluronic F127, MW 12600, CAS 9003-11-6), poly(d,l-lactic acid-co-glycolide) (PLGA (50 : 50), MW 7000–17000, CAS 26780-50-7) were purchased from Sigma-Aldrich Company. Glucose oxidase (GOx, 100–250 U mg−1, CAS 9001-37-0) was purchased from Shanghai Yuanye Biotechnology Co. Ltd. Ammonia (NH3·H2O, 25%, CAS 1336-21-6), acetone (C3H6O, 97%, CAS 67-64-1), n-octane (n-C8H18, 96%, CAS 111-65-9), chloroform (CHCl3, 97%, CAS 67-66-3), hydrogen peroxide (H2O2, 30%, CAS 7722-84-1), terephthalic acid (TA, 99%, CAS 100-21-0), citric acid monohydrate (CA·H2O, 99%, CAS 5949-29-1), potassium dihydrogen phosphate (KH2PO4, 99%, CAS 7778-77-0), disodium hydrogen phosphate (Na2HPO4, 99%, CAS 7558-79-4), ethanol (C2H5OH, 99.5%, CAS 64-17-5) and methanol (CH3OH, 99.5%, CAS 67-56-1), were analytically pure and obtained from Sinopharm reagent company. All reagents were analytical grade and were used directly without further purification. Throughout, Milli-Q ultrapure water was used in all needed experiments.
Wang Y., Li X., Fang Y., Wang J., Yan D, & Chang B. (2023). Degradable Fe3O4-based nanocomposite for cascade reaction-enhanced anti-tumor therapy. RSC Advances, 13(12), 7952-7962.
Publication 2023
3,3',5,5'-tetramethylbenzidine Acetone Albumins Ammonia calcein AM Chloroform Citric Acid Monohydrate Eagle Ethanol Ethers ferric chloride hexahydrate ferrous chloride Fetal Bovine Serum Fetus fluorexon Glucose HeLa Cells Methanol octane Oleic Acid Oxidase, Glucose Oxides Peroxide, Hydrogen Phosphates Pluronic F-127 poly-d,l-lactic acid Polyethylene Glycols Polylactic Acid-Polyglycolic Acid Copolymer potassium phosphate, monobasic Propidium Iodide pyrroline Saline Solution Serum Albumin, Bovine sodium phosphate, dibasic terephthalic acid Trypsin
4
Comprehensive T2DM Metabolic Profiling
A trained interviewer recorded patients’ information, including demographic characteristics, duration of T2DM, and medical history. The height and weight of patients without shoes and with light clothes to the nearest 100 g were measured using the same device (Seca weighing scale, made in Germany), and body mass index (BMI) was calculated by dividing weight (kg) into height squared (meters).
After 8 to 12 h of overnight fasting, a blood sample was taken from all participants to measure cell counts, iron profiles (total iron-binding capacity (TIBC), hemoglobin concentration, serum ferritin, and iron level), triglyceride, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), fasting plasma glucose (FPG), and glycated hemoglobin (Hb A1c). Also, urine sample was taken from the participants. All of them were measured in a same lab with same kits, devices, and methods.
FPG was measured by the colorimetric glucose oxidase method (Human, Heidelberg Germany). Furthermore, TC, HDL-C, and triglyceride were measured by enzymatic method using a proper kit (Lipid, Pars Azmoon Co., Karaj, Iran). Also, if triglyceride levels were < 400 mg/dL, the Friedewald formula (LDL = total cholesterol - (HDL + TG/5)) was applied for calculating LDL-C, and if triglycerides were ≥ 400 mg/dL, LDL-C was measured by direct assay. Hb A1C was assessed by column chromatography. TIBC was measured by the chemical precipitation method. Also, ferritin was measured by the immunoassay method using a gamma counter. Urinary albumin excretion was assessed by the immunoturbidometry method of a 24 hour’s urine collection. Serum creatinine levels were measured using kinetic colorimetric Jaffe with a sensitivity of 0.2 mg/dL (range, 0.2–15 mg/dL). The Modification of Diet in Renal Disease (MDRD) equation was employed for calculating the estimated glomerular filtration rate (e-GFR) of the participants [15 , 16 (link)].
Hizomi Arani R., Fakhri F., Naeimi Tabiee M., Talebi F., Talebi Z., Rashidi N, & Zahedi M. (2023). Prevalence of anemia and its associated factors among patients with type 2 diabetes mellitus in a referral diabetic clinic in the north of Iran. BMC Endocrine Disorders, 23, 58.
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Publication 2023
Albumins Biological Assay BLOOD Chemical Precipitation Cholesterol Cholesterol, beta-Lipoprotein Chromatography Colorimetry Creatinine Enzymes Ferritin Gamma Rays Glomerular Filtration Rate Glucose Hemoglobin Hemoglobin, Glycosylated Hemoglobin A, Glycosylated High Density Lipoprotein Cholesterol Homo sapiens Hypersensitivity Immunoassay Index, Body Mass Interviewers Iron Kidney Diseases Kinetics Light Lipids Medical Devices Oxidase, Glucose Patients Plasma Poly(ADP-ribose) Polymerases Serum Therapy, Diet Triglycerides Urine Urine Specimen Collection
5
Droplet-based Enzymatic Assay and Cell Labeling
Fluorinated oil (HFE7500, 3 M, USA) supplemented with 0.5% (w/w) surfactant (RAN Biotechnologies, USA) was used as the continuous phase. To visually differentiate the two sets of droplets, deionized (DI) water was used as the first dispersed phase to generate large droplets, while 12 mM fluorescent dye solution (Rhodamine 6 G, Sigma‒Aldrich, USA) was used as the second dispersed phase to generate small droplets. The interfacial tension is 6.8 mN/m. During droplet synchronization, the interfacial tension forces help resist changes in droplet shape, preventing droplet breakup.
For enzymatic reactions, glucose oxidase (G7141), D-(+)-glucose (G7021), 4-AAP (A4382), and TOPS (E8506) were all purchased from Sigma‒Aldrich, and HRP (31490) was purchased from Thermo Scientific. The concentrations of glucose oxidase, HRP, 4-AAP, and TOPS were maintained at 200 U/mL, 1200 U/mL, 0.6 mol/L, and 1 mol/L, respectively, while the concentration of glucose was set at 100, 200, 300, and 400 mg/dL.
Mouse myeloma cells (SP2/0 cell line) were used as the model cells for cell-bead pairing. They were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium (Life Technologies, USA) supplemented with 10% fetal bovine serum (FBS, A4766801, Gibco, USA) and 100 U/mL penicillin/streptomycin (Gibco, USA) in a humidified atmosphere with 5% CO2 at 37 °C. After trypsin treatment, the cells were resuspended in 2 μM Calcein AM (C3100MP, Invitrogen, USA) in FBS-free cell culture medium for 30 min to enable live cell staining. Then, the cells were centrifuged, and 15% (v/v) OptiPrep (D1556, Sigma‒Aldrich, USA) in cell culture medium, which had the same density as the SP2/0 cells, was used for resuspension of the cells.
Nan L., Mao T, & Shum H.C. (2023). Self-synchronization of reinjected droplets for high-efficiency droplet pairing and merging. Microsystems & Nanoengineering, 9, 24.
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Publication 2023
Atmosphere Cell Culture Techniques Cell Lines Cells Culture Media Enzymes Fluorescent Dyes fluorexon Glucose Interfacial Force Multiple Myeloma Mus Oxidase, Glucose Penicillins Rhodamine Streptomycin Surface-Active Agents Trypsin

Top products related to «Oxidase, Glucose»

1
Glucose oxidase by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, France, United Kingdom
Glucose oxidase is an enzyme that catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. It is commonly used in various laboratory applications, such as the detection and measurement of glucose levels in biological samples.
2
Catalase by Merck Group
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Catalase is a common enzyme found in the cells of most living organisms. It functions as a catalyst, accelerating the decomposition of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2).
3
Amplex red glucose glucose oxidase assay kit by Thermo Fisher Scientific
Sourced in United States, Germany
The Amplex Red Glucose/Glucose Oxidase Assay Kit is a fluorometric assay kit designed to detect and quantify glucose levels in samples. The kit utilizes the Amplex Red reagent, which reacts with hydrogen peroxide produced by the glucose oxidase enzyme, resulting in a fluorescent product that can be measured.
4
D glucose by Merck Group
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D-glucose is a type of monosaccharide, a simple sugar that serves as the primary source of energy for many organisms. It is a colorless, crystalline solid that is soluble in water and other polar solvents. D-glucose is a naturally occurring compound and is a key component of various biological processes.
5
Trolox by Merck Group
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Trolox is a water-soluble vitamin E analog that functions as an antioxidant. It is commonly used in research applications as a reference standard for measuring antioxidant capacity.
6
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
7
Beckman glucose analyzer by Beckman Coulter
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The Beckman Glucose Analyzer is a laboratory instrument designed to measure the concentration of glucose in biological samples. It utilizes electrochemical detection methods to provide accurate and reliable glucose measurements.
8
Glucose oxidase method by Beckman Coulter
Sourced in United States
The glucose oxidase method is a laboratory technique used to measure the concentration of glucose in a sample. It involves the enzymatic conversion of glucose to gluconic acid and hydrogen peroxide, which is then detected and quantified. This method provides a reliable and accurate way to determine glucose levels in various biological samples.
9
Streptozotocin (stz) by Merck Group
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STZ is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and experiments. The core function of STZ is to serve as a tool for carrying out specific tasks or procedures in a laboratory setting. No further details or interpretation of its intended use are provided.
10
Catalase by Roche
Sourced in Germany
Catalase is a common enzyme found in the cells of most living organisms. It acts as a catalyst, accelerating the decomposition of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). This function helps to protect cells from the potentially damaging effects of hydrogen peroxide.

More about "Oxidase, Glucose"

Oxidase and Glucose: Vital Biomolecules in Metabolic Processes Oxidase and glucose are two crucial biomolecules that play vital roles in various metabolic processes.
Oxidase, also known as oxidoreductase, catalyzes the oxidation of substrates, often involving the transfer of electrons.
This enzymatic activity is essential for energy production, signaling pathways, and detoxification mechanisms within cells.
Glucose, on the other hand, is a primary source of energy for many cells.
As a monosaccharide, glucose is the most abundant carbohydrate in the body and serves as a vital fuel for cellular respiration.
Glucose oxidase, a specific form of oxidase, catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide, making it a valuable tool in research and analytical applications.
Researchers studying oxidase, glucose, and related compounds can leverage the AI-driven platform of PubCompare.ai to optimize their workflows.
The platform helps locate accurate and reproducible protocols from literature, preprints, and patents, and provides AI-powered comparisons to identify the best methods and products for experiments.
This can lead to seamless research optimization and enhanced reproducibility, ultimately advancing scientific understanding and discoveries.
Other related terms and concepts that may be relevant include catalase, which is an enzyme that catalyzes the decomposition of hydrogen peroxide; Amplex Red Glucose/Glucose Oxidase Assay Kit, a fluorometric method for detecting glucose; D-glucose, the primary form of glucose found in the body; Trolox, a water-soluble vitamin E analog used as an antioxidant; bovine serum albumin, a common protein used in various biochemical assays; Beckman Glucose Analyzer, a device used for measuring glucose levels; the glucose oxidase method, a common technique for quantifying glucose; and streptozotocin (STZ), a compound used to induce diabetes in animal models.
By understanding the significance of oxidase and glucose, and leveraging the capabilities of PubCompare.ai, researchers can streamline their workflows, enhance the accuracy and reproducibility of their experiments, and contribute to the advancement of scientific knowledge in this field.
OtherTerms: Oxidoreductase, Carbohydrate, Energy Metabolism, Catalytic Activity, Hydrogen Peroxide, Gluconic Acid, Fluorometric Assay, Antioxidant, Biochemical Assay, Diabetes