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> Chemicals & Drugs > Amino Acid > Phalloidine

Phalloidine

Phalloidine is a natural compound found in certain mushroom species that has been extensively studied for its potential applications in biology and medicine.
It is known to selectively bind to and stabilize actin filaments, making it a valuable tool for studying cytoskeleton dynamics and cell motility.
Phalloidine research has yielded important insights into a wide range of cellular processes, including cell signaling, vesicle trafficking, and muscle contraction.
Researchers utilize advanced techniques, such as high-throughput screening and AI-driven analysis, to optimie Phalloidine research protocols and maximize the reproducibility and impact of their findings.
This powerful compound continues to be a focus of intense scientific inquiry, with the potential to unlock new discoveries and therapeutic applications.

Most cited protocols related to «Phalloidine»

1
Transwell Invasion and ECM Degradation Assay
Cited 317 times
Transwell invasion assay was performed as described previously4 (link). In brief, cells were loaded onto the upper well of the Transwell chamber with 8 µm ϕ pore membrane (Coster), precoated with Matrigel on an upper side of the chamber. The lower well was filled with 600 µl of DMEM containing 10% FBS. After incubation for 24 hr, cells invaded to lower surface of the membrane were counted. For ECM degradation assay, glass coverslips were coated with gelatin conjugated with either Alexa Fluor 594 (Invitrogen) (Alexa-gelatin) or fluorescein (Invitrogen) (FL-gelatin) as described65 (link). Transfected cells were trypsinized, replated on these glass coverslips, and cultured for 6 hr. After fixation, cells were fixed and stained with phalloidin. Number of invadopodia, identified as F-actin dots in the areas of degraded gelatin, was quantified by using the ImageJ particle analysis tool.
Nishita M., Park S.Y., Nishio T., Kamizaki K., Wang Z., Tamada K., Takumi T., Hashimoto R., Otani H., Pazour G.J., Hsu V.W, & Minami Y. (2017). Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Scientific Reports, 7, 1.
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Publication 2017
Alexa594 Biological Assay Cells F-Actin Fluorescein Gelatins matrigel Phalloidine Podosomes Tissue, Membrane
2
Fluorescent Imaging of Golgi Organization
Cited 151 times
Cells were cultured on glass coverslips (Matsunami) pre-coated with 10 µg/ml fibronectin (Sigma) and fixed with 4% (w/v) paraformaldehyde in PBS or BRB80 [80 mM Pipes (pH 6.8), 1 mM MgCl2, and 1 mM EGTA] for 10 min at room temperature. Fixed cells were stained with the respective antibodies, phalloidin conjugated with either Alexa Fluor 488 or rhodamine (Invitrogen), along with DAPI (Sigma) as described previously2 (link), 54 (link). In situ proximity ligation assay (PLA) was performed using Duolink kit (Olink Bioscience) according to the manufacturer’s instructions. After completion of the PLA reaction, samples were refixed with 4% (w/v) paraformaldehyde and incubated with Alexa Fluor-conjugated secondary antibodies (Life Technologies) to detect the individual proteins. Fluorescence images were obtained using a laser scanning confocal imaging system (LSM700, Carl Zeiss) and processed using the ImageJ software. Number of Golgi fragments was quantified by using the ImageJ particle analysis tool. Colocalization was examined using the ImageJ JACoP plugin64 (link) or Metamorph (Molecular Devices).
Nishita M., Park S.Y., Nishio T., Kamizaki K., Wang Z., Tamada K., Takumi T., Hashimoto R., Otani H., Pazour G.J., Hsu V.W, & Minami Y. (2017). Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Scientific Reports, 7, 1.
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Publication 2017
alexa fluor 488 Antibodies Biological Assay Cells DAPI Egtazic Acid Fluorescence FN1 protein, human Golgi Apparatus Ligation Magnesium Chloride Medical Devices paraform Phalloidine piperazine-N,N'-bis(2-ethanesulfonic acid) Proteins Rhodamine
3
Visualization of Endogenous Myosin in Drosophila Embryos
Cited 38 times
Heat fixation and staining with anti-myosin heavy chain (MHC) antibody did not preserve the normal organization of apical myosin observed in live squ-GFP26 (link), squ-mCherry (Myosin-mCherry), and GFP-zipper (GFP-MHC)27 (link) embryos. Therefore, endogenous GFP fluorescence was used to visualize myosin. squ-GFP embryos were fixed with 10% paraformaldehyde/heptane for 20 minutes, manually devitellinized, stained with Alexa-568 Phalloidin (Invitrogen) to visualize actin, and mounted in AquaPolymount (Poysciences, Inc.).
Martin A.C., Kaschube M, & Wieschaus E.F. (2008). Pulsed actin-myosin network contractions drive apical constriction. Nature, 457(7228), 495.
Publication 2008
Actins alexa 568 Antibodies, Anti-Idiotypic Embryo Fluorescence Heptane Myosin ATPase Myosin Heavy Chains paraform Phalloidine
4
Actin Polymerization and Bacterial Motility
Cited 28 times
Compounds were purchased from Chemdiv, San Diego CA: CK-0944636 (Catalog number 8012-5103), CK-0993548 (Catalog number K205-1650), CK-0944666 (Catalog number 8012-5153) and CK-0157869 (Catalog number K205-0942). We purified native Arp2/3 complex from human platelets12 (link), bovine thymus6 (link), Schizosaccharomyces pombe13 (link) and Saccharomyces cerevisiae (Supplemental methods), actin from chicken skeletal muscle14 (link) and recombinant HsWASp, WASp105-502, WASp-VCA and Cdc4212 (link), N-WASp-VCA 428-505 (Supplemental methods), GST-ActA 36-170 (Supplemental methods) and S. pombe Cdc12p(FH2)-His 973-139015 (link) from E. coli. We used standard assays to measure polymerization of pyrenyl-actin16 (link) and to visualize actin filaments by fluorescence microscopy17 (link). Binding of etheno-ATP to Arp2/3 complex was performed as described previously with slight modifications18 (link). We crystallized BtArp2/3 complex7 (link) with either 0.5 mM CK-548 or 1 mM CK-636 in DMSO or soaked these compounds into crystals for 24 hours before freezing in liquid nitrogen. Diffraction data were collected at beamline X29A at Brookhaven National Laboratories. SKOV3 cells were infected with Listeria monocytogenes and fixed with 2% formaldehyde, permeabilized with 0.1% Triton-X in PBS, stained with Listeria antibody (US Biologics, Cleveland, Ohio) and Alexa Fluor 568 phalloidin (Molecular Probes, Eugene, OR), and imaged by fluorescence microscopy. We used an Isodata threshold on background-subtracted images of Listeria to isolate individual bacterium and measure the ratio of colocalized actin to Listeria fluorescence. Monocyte THP-1 cells were differentiated in 50 nM phorbol myristate acetate (Sigma-Aldrich-Fluka) to form podosomes before treatment with compounds. Black molly keratocytes19 (link) were observed by time-lapse phase contrast microscopy.
Nolen B.J., Tomasevic N., Russell A., Pierce D.W., Jia Z., McCormick C.D., Hartman J., Sakowicz R, & Pollard T.D. (2009). Characterization of two classes of small molecule inhibitors of Arp2/3 complex. Nature, 460(7258), 1031-1034.
Publication 2009
Actin-Related Protein 2-3 Complex Actins alexa 568 Bacteria Biological Assay Biological Factors Cattle Cells Chickens CK-0944636 CK-0944666 CK-0993548 Escherichia coli Fluorescence Formaldehyde Homo sapiens Immunoglobulins Isoenzyme CPK MB Listeria Listeria monocytogenes Microfilaments Microscopy, Fluorescence Microscopy, Phase-Contrast Molecular Probes Molly Monocytes Nitrogen Phalloidine Podosomes Polymerization Saccharomyces cerevisiae Schizosaccharomyces Schizosaccharomyces pombe Skeleton Sulfoxide, Dimethyl Tetradecanoylphorbol Acetate THP-1 Cells WASL protein, human WAS protein, human
5
Cas9-Mediated Genome Editing in Zebrafish
Cited 27 times
The full length of humanized Cas9 cDNA was cloned into the PXT7 vector and linearized, and capped mRNA was synthesized using Ambion mMESSAGE mMACHINE mRNA transcription synthesis kits. Cas9 mRNA (300 ng/μl) and gRNA (20 ng/μl) were co-injected into one-cell-stage wild-type or Tg(fli1:EGFP) transgenic embryos. Single-stranded donor oligonucleotides were also co-injected for examining HDR. Injected embryos were incubated at 28.5 °C for examination of phenotypes. Embryos were fixed for phalloidin staining at 60 hpf, or were collected for making genomic DNA for genotyping at 50 hpf. Photographs were taken using a Leica DM5000B fluorescence microscope and/or a Zeiss 700 confocal microscope.
Chang N., Sun C., Gao L., Zhu D., Xu X., Zhu X., Xiong J.W, & Xi J.J. (2013). Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos. Cell Research, 23(4), 465-472.
Publication 2013
Anabolism Animals, Transgenic Cells Cloning Vectors DNA, Complementary Embryo Genome Microscopy, Confocal Microscopy, Fluorescence Oligonucleotides Phalloidine Phenotype RNA, Messenger Tissue Donors Transcription, Genetic

Most recents protocols related to «Phalloidine»

1
Bioceramic Composition Enhances Cell Adhesion
Not available on PMC !

Example 8

Cell adhesion was also evaluated by means of in vitro scratch wound-healing assay. HDPSCs cells were analyzed by difference in staining with phalloidin (cell nucleus) and DAPI to visualize actin cytoskeleton.

Cell adhesion results showed excellent interaction and adhesion between neighboring cells in the presence of bioceramic composition. The Bioceramic composition sealer (CB5) and Bioceramic composition repair (CB6), showed a gradual increase in growth over time, an extended morphology and a high content of F-Actin (cell microfilamen), reaching confluence after 72 hours of culture.

The analysis of cell proliferation (via cell viability study), apoptosis, cell adhesion and morphology (via cell adhesion study) and migration (via cell migration study) showed very positive results, indicating that the proposed bioceramic composition induces the odonto/osteogenic mineralization and differentiation process in the presence of tooth-specific human stem cells (hDPSCs pulp). While a market resin sealer was also used in the comparative studies, however, all results were not satisfactory for this product.

US11857558B2. Dental and medical compositions having a multiple source of metallic ions (2024-01-02). ANGELUS INDÚSTRIA DE PRODUTOS ODONTOLÓGICOS S/A [BR]. Inventors: César Eduardo Bellinati [BR], Cristiane Vila [BR], William Pereira Dos Santos [BR], Maíra Bendlin Calzavara [BR].
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Patent 2024
Apoptosis Biological Assay Cell Adhesion Cell Nucleus Cell Proliferation Cell Survival DAPI Dental Pulp Differentiations, Cell F-Actin Homo sapiens Microfilaments Migration, Cell Osteogenesis Phalloidine Physiologic Calcification Resins, Plant Stem, Plant Stem Cells Tooth
2
Intracellular Visualization Using Two-Photon Microscopy

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Publication 2023
Acceleration Acetone Alexa594 Anti-Antibodies Buffers cadherin 5 Cells DAPI Dehydration F-Actin fluorescein isothiocyanate-phalloidin Glutaral Gold IGF II Immunoglobulins Microscopy Palladium Phalloidine Phosphates Polaron Proteins Protoplasm Reconstructive Surgical Procedures Sapphire Technique, Dilution tetramethylrhodamine isothiocyanate Triton X-100 Vacuum
3
Phalloidin and DAPI Staining

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Publication 2023
Alexa Fluor 555 Cells DAPI Microscopy Phalloidine
4
Evaluating BM-MSC Polarization and Morphology
For the evaluation of BM-MSC polarization and the cellular morphology, upon TP treatment, the cells were seeded in 8-well chamber slides (SPL Life Sciences, #30108) at a density of 2x104 cells per well and incubated overnight to allow attachment, followed by treatment with 10-8 M TP for 24 hours. The slides were fixed and permeabilized as previously described23 and labeled either with anti-CXCL9 PE (Clone: J1015E10, Biolegend, #357903) and anti-CXCL5 APC (Clone: J111B7, Biolegend, #524105) antibodies or with F-actin probe Phalloidin (Alexa Fluor 555 conjugated, Thermo Fisher Scientific, #A34055) by incubating overnight at 4 °C. Cell nuclei were counterstained with DAPI, and the slides were mounted with FluoroShield medium (Sigma Aldrich, #F6182). Micrographs were taken with a Zeiss LSM 780 confocal microscope.
Aru B., Akdeniz T., Dağdeviren H., Gürel G, & Yanıkkaya Demirel G. (2023). Testosterone Propionate Promotes Proliferation and Viability of Bone Marrow Mesenchymal Stem Cells while Preserving Their Characteristics and Inducing Their Anti-Cancer Efficacy. Balkan Medical Journal, 40(2), 117-123.
Publication 2023
Alexa Fluor 555 Antibodies Cell Nucleus Cells Clone Cells CXCL5 protein, human CXCL9 protein, human DAPI F-Actin Fluoroshield Microscopy, Confocal Phalloidine
5
Immunolabeling of Neuronal Cytoskeleton
Neurons were seeded on poly-L-lysine-coated #1.5 coverslips (633029; Carolina Biological Supply). At indicated timepoints, coverslips were briefly washed in Dulbecco’s PBS with calcium and magnesium (PBL02; Caisson Labs), and fixed for 15 min with 4% paraformaldehyde (diluted in PBS from 16%, Electron Microscopy Services, 15710) and 4% sucrose in PBS at 37°C. For immunolabeling of fascin, coverslips were washed and fixed for 10 min with ice-cold methanol at −20°C. Subsequent steps were performed at room temperature. Autofluorescence was quenched by incubation with 50 mM NH4Cl for 10 min at room temperature. Coverslips were blocked and permeabilized using a buffer of 10% goat serum and 0.1% Triton X-100 in PBS and incubating for 30 min. Antibodies were diluted in a buffer of 3% goat serum and 0.1% Triton X-100 in PBS. Coverslips were incubated with primary antibodies for 1 h, washed three times in PBS with 0.1% Tween-20, and incubated with secondary antibodies and Alexa Fluor 488 phalloidin (A12379; Thermo Fisher Scientific, 1:40) for 30 min. Coverslips were mounted using Prolong Gold Antifade (P36930; Thermo Fisher Scientific), and allowed to cure for at least 24 h prior to imaging. Antibodies used were as follows: mouse monoclonal anti-Arp2/3 complex (#MABT95; Millipore, RRID:AB_11205567; 1:250), mouse monoclonal anti-fascin (#54545; Cell Signaling Technology, RRID:AB_2799464; 1:50), rabbit monoclonal anti-β-III-tubulin (#5568; Cell Signaling, Clone D71G9, RRID:AB_10694505; 1:100), rabbit monoclonal anti-MAP2 (#8707; Cell Signaling, Clone D5G1, RRID:AB_2722660; 1:500), goat anti-rabbit Alexa Fluor 568 (#A-11011; Molecular Probes, RRID:AB_143157; 1:1,000), goat anti-rabbit Alexa Fluor 647 (#A-21245; Thermo Fisher Scientific, RRID:AB_2535813; 1:1,000).
Parker S.S., Ly K.T., Grant A.D., Sweetland J., Wang A.M., Parker J.D., Roman M.R., Saboda K., Roe D.J., Padi M., Wolgemuth C.W., Langlais P, & Mouneimne G. (2023). EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons. The Journal of Cell Biology, 222(5), e202106081.
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Publication 2023
Actin-Related Protein 2-3 Complex alexa 568 alexa fluor 488 Alexa Fluor 647 Antibodies Biopharmaceuticals Buffers Calcium Clone Cells Cold Temperature Electron Microscopy fascin Goat Gold Lysine Magnesium MAP2 protein, human Methanol Molecular Probes Mus Neurons paraform Phalloidine Poly-5 Rabbits Serum Sucrose Triton X-100 Tubulin Tween 20

Top products related to «Phalloidine»

1
Alexa fluor 488 phalloidin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Canada, Japan, China, France, Italy, Spain, Israel, Australia, Austria
Alexa Fluor 488 phalloidin is a fluorescent dye that selectively binds to F-actin, a component of the cytoskeleton. It is used in microscopy and flow cytometry applications to visualize and study the distribution and organization of actin filaments within cells.
2
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Italy, Australia, France, Switzerland, Spain, Belgium, Denmark, Panama, Poland, Singapore, Austria, Morocco, Netherlands, Sweden, Argentina, India, Finland, Pakistan, Cameroon, New Zealand
DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
3
4 6 diamidino 2 phenylindole (dapi) by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore
DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
4
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, France, Canada, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
5
Alexa fluor 488 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland
Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
6
Hoechst 33342 by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, France, Canada, Spain, Belgium, Italy, Australia, Austria, Denmark, Netherlands, Switzerland, Ireland, New Zealand, Portugal, Brazil, Argentina, Singapore, Poland, Ukraine, Macao, Thailand, Finland, Lithuania, Sweden
Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
7
Alexa fluor 568 phalloidin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Switzerland, France, Australia
Alexa Fluor 568 phalloidin is a fluorescent dye used for the detection and visualization of F-actin in cells. It binds specifically to F-actin, allowing for the labeling and imaging of the actin cytoskeleton. The Alexa Fluor 568 dye exhibits bright red fluorescence when excited at the appropriate wavelength.
8
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
9
Alexa fluor 647 phalloidin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Switzerland
Alexa Fluor 647 phalloidin is a fluorescent conjugate used for the high-affinity labeling of F-actin in fixed and permeabilized cells. It is a useful tool for visualizing the cytoskeleton in microscopy applications.

More about "Phalloidine"

Phalloidin, a natural compound found in certain mushroom species, has been extensively studied for its potential applications in biology and medicine.
This powerful molecule is known to selectively bind to and stabilize actin filaments, making it a valuable tool for studying cytoskeleton dynamics and cell motility.
Phalloidin research has yielded important insights into a wide range of cellular processes, including cell signaling, vesicle trafficking, and muscle contraction.
Researchers utilize advanced techniques, such as high-throughput screening and AI-driven analysis, to optimize Phalloidin research protocols and maximize the reproducibility and impact of their findings.
Alexa Fluor 488 phalloidin, a fluorescent conjugate of phalloidin, is commonly used to visualize and quantify actin filaments, while DAPI and Triton X-100 are often used in conjunction with Alexa Fluor 488 phalloidin for nuclear staining and permeabilization, respectively.
Hoechst 33342, another nuclear stain, is also frequently employed in Phalloidin-based studies.
Alexa Fluor 568 phalloidin and Alexa Fluor 647 phalloidin offer alternative fluorescent labels for actin filament detection, providing researchers with a range of options to suit their experimental needs.
Bovine serum albumin (BSA) is often used as a blocking agent to reduce non-specific binding in Phalloidin-based assays.
This powerful compound continues to be a focus of intense scientific inquiry, with the potential to unlock new discoveries and therapeutic applications.
PubCompare.ai, an innovative platform, optimizes Phalloidin research protocols with AI-driven reproducibility, helping researchers easily locate the best protocols from literature, pre-prints, and patents using its compariosn tools.
By leveraging AI-powered insights and data-driven decision making, researchers can boost their Phalloidin research and drive meaningful advances in the field.