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Proto-Oncogene Proteins c-fos

Proto-Oncogene Proteins c-fos: A critical regulator of cellular proliferation and differentiation, the c-fos proto-oncogene is a memeber of the immediate early gene family.
Its protein product, c-Fos, forms part of the transcription factor complex AP-1, which plays a key role in mediating cellular responses to a variety of extracellular stimuli.
Dysregulation of c-fos expression has been implicated in the developement of certain cancers, making it an important target for biological research and therapeutic interventions.
Explore the latest protocols and leverage AI-powered comparisons with PubCompare.ai to streamline your c-fos research and optimize your experimental approach.

Most cited protocols related to «Proto-Oncogene Proteins c-fos»

1
Optogenetic Modulation of Stress Response
Cited 7 times
Wild-type mice received a series of footshocks (0.5 mA for 2 s, ten times in 5 min; SMSCK, Kinder Scientific) and their activity was video-recorded for 15 min in the footshock chamber, in a novel environment or in the HC immediately after the footshock. For in vivo optogenetics experiments, mice were handled the 4 days preceding behavioural assessment. Blue light was delivered for 5 min (10 Hz, 10 ms pulse width, 15 mW; 473 nm); in LH stimulation experiments, 20 Hz was used. Yellow light (15 mW) was delivered continuously for 15 or 5 min. Behavioural video analysis was conducted by an individual blinded to subject treatment group using a macro in Microsoft Excel. Walking was noted as the animal changed its location or turned as long as the front paws moved. Freezing behaviour was noted if animal showed no movement for at least 3 s, except respiratory movements.
For c-Fos immunolabelling, animals were killed 2 h after light stimulation. For experiments with Arch3.0, after recovery mice were handled for 4 days and HAB to the experimental condition for 3 additional days. After a similar series of footshock, their behaviour was recorded in their HC under continuous yellow light (15 mW) for 15 min. For assessing the involvement of circulating CORT, metyrapone (75 mg per kg, Tocris Bioscience, dissolved in 50 μl polyethylene glycol) was administered i.p. 60 min before footshock. For circulating CORT level measurements, baseline blood samples were taken from the tail vein at least 2 h before light stimulation. Second sampling was done 15 min after the onset of light stimulation. CORT level was measured using an ELISA kit (Arbor Assays). To investigate social cues bedding was not replaced in the HC of mice for 10 days prior testing. Experiment subjects were at the age of 16 weeks while conspecific males were 7 weeks old.
Füzesi T., Daviu N., Wamsteeker Cusulin J.I., Bonin R.P, & Bains J.S. (2016). Hypothalamic CRH neurons orchestrate complex behaviours after stress. Nature Communications, 7, 11937.
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Publication 2016
Animals Biological Assay BLOOD Cortisone Enzyme-Linked Immunosorbent Assay Light Males Metyrapone Mice, House Movement Optogenetics Photic Stimulation Polyethylene Glycols Proto-Oncogene Proteins c-fos Pulse Rate Respiratory Rate Tail Veins
2
Brain Tissue Extraction and Analysis
Cited 7 times
To obtain brain tissue for Experiment 2, rats were deeply anesthetized with pentobarbital and perfused with 0.1 M PBS, followed by 4% paraformaldehyde, 4°C; pH=7.4. The brains were removed from the skull and placed in the same fixative solution for approximately 24 h. Next, they were transferred to 30% (w/v) sucrose in a 0.1 M PBS solution, and subsequently sliced on a freezing microtome into 40 μm coronal sections. Tissue was then stored in cryoprotectant (−20°C) until immunohistochemistry (IHC) processing. IHC staining and quantification procedures were similar to those we have previously described (Cannady et al., 2011 (link); Besheer et al., 2012a (link); Besheer et al., 2014 (link)). Free-floating coronal sections were incubated in rabbit anti-Fluorogold antibody (1:8,000; Millipore) for 24 h or rabbit anti-c-Fos antibody (1:20,000; Millipore) for 48 h at 4 °C with agitation. The brain regions examined were the prelimbic region of the medial prefrontal cortex (mPFC; AP +4.2 to +3.2 mm), anterior insular cortex (IC; +2.8 to +1.9 mm), and nucleus accumbens core (AcbC; AP −2.3 to −1.3) and rhomboid thalamic nucleus (Rh; AP −1.8 to −3.2 mm), according to (Paxinos & Watson, 2007 ). Images were acquired utilizing Olympus CX41 light microscope (Olympus America) and analyzed utilizing Image-Pro Premier image analysis software (Media Cybernetics, MD). IR data (c-Fos positive pixels/mm2) were acquired from a minimum of three sections/brain region/animal, and the data were averaged to obtain a single value per subject.
Jaramillo A.A., Randall P.A., Frisbee S, & Besheer J. (2016). Modulation of sensitivity to alcohol by cortical and thalamic brain regions. The European journal of neuroscience, 44(8), 2569-2580.
Publication 2016
Animals anti-c antibody Antibodies, Anti-Idiotypic Brain Cranium Cryoprotective Agents Fixatives Fluoro-Gold Insula of Reil Light Microscopy Microtomy Nucleus Accumbens paraform Pentobarbital POU3F2 protein, human Prefrontal Cortex Proto-Oncogene Proteins c-fos Rabbits Rattus Rhomboidal Nucleus Sucrose Tissues
3
Quantification of c-Fos and Zif268 in Amygdalar Interneurons
Cited 7 times
Mice were killed 2 h after the start of the extinction retrieval session [time interval according to postextinction c-Fos and Zif268 data obtained previously (Herry and Mons, 2004 (link))]. Mice were deeply anesthetized with an overdose of sodium pentobarbital (200 mg/kg) and transcardially perfused with 20 ml of 0.9% saline followed by 20 ml of 4% paraformaldehyde in 0.1 mol/L phosphate buffer, pH 7.4. Brains were then removed and postfixed at 4°C overnight in4%paraformaldehyde in phosphate buffer. Brains were sectioned in the coronal plane at 50 µm thickness on a vibratome (VT1000S, Leica Microsystems) and collected in immunobuffer. The free-floating sections were processed for c-Fos immunoreactivity as described previously (Singewald et al., 2003 (link)), via incubation with a polyclonal primary antibody (1:10,000; sc-52; Santa Cruz Biotechnology), and for Zif268-like immunoreactivity as described previously (Hefner et al., 2008 (link)), via incubation with a polyclonal primary antibody (1:5000; sc-189; Santa Cruz Biotechnology ) and a biotinylated goat anti-rabbit secondary antibody (1:200; Vector Laboratories).
The anatomical localization of c-Fos-positive or Zif268-positive cells was aided by using the illustrations in a stereotaxic atlas (Paxinos and Franklin, 2001 ). Zif268-positive neurons in intercalated cell masses (ITCs) were identified with reference to published studies in the rat and mouse (Millhouse, 1986 (link); Berretta et al., 2005 (link); Marowsky et al., 2005 (link); Geracitano et al., 2007 (link); Hefner et al., 2008 (link)). Specifically, one mass of ITC cells situated along the external capsule at the junction of the La and the BA was labeled as lateral paracapsular ITC neurons (Ilp). A second mass of ITC cells was observed along the intermediate capsule at the junction of La and BA and lateral to the CeA and was defined as the Imp. The main ITC nucleus was defined as described previously (Paxinos and Franklin, 2001 ). Since recent evidence (Busti et al., 2010 ) points to differential connectivity of these three cell groups, we quantified IEG expression separately in these groups. Unless otherwise stated, all c-Fos-positive or Zif268-positive cells that were distinguishable from background staining were bilaterally counted in each region of interest within a defined area (0.01 mm2). Counts were averaged from two to four sections per mouse (depending on the brain area under investigation) and presented as cells/0.01 mm2.
Whittle N., Hauschild M., Lubec G., Holmes A, & Singewald N. (2010). Rescue of Impaired Fear Extinction and Normalization of Cortico-Amygdala Circuit Dysfunction in a Genetic Mouse Model by Dietary Zinc Restriction. The Journal of Neuroscience, 30(41), 13586-13596.
Publication 2010
Antibodies, Anti-Idiotypic Brain Buffers Capsule Cell Nucleus Cells Cloning Vectors Drug Overdose External Capsule Extinction, Psychological Goat Immunoglobulins Interneurons Mus Neurons Normal Saline paraform Pentobarbital Sodium Phosphates Proto-Oncogene Proteins c-fos Rabbits
4
Molecular Expression Analysis in Cells
Cited 6 times
After 18-h incubation with samples in 6-well plates, the mRNA expression of STAT1, STAT3, C/EBP homologous protein (CHOP; GADD153; DDIT3), JAK2, first apoptosis signal receptor (FAS), c-Fos, nitric oxide synthase 2 (NOS2), and prostaglandin-endoperoxide synthase 2 (PTGS2; COX2) in each well containing 300,000 cells were evaluated with real time RT-PCR using Experion Automatic Electrophoresis System (Bio-Rad) and Bio-Rad CFX 96 Real-Time PCR Detection System (Bio-Rad) [10 (link),11 (link)]. Cells were lysed and Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Duren, Germany) according to the manufacturer’s instructions. The β-Actin was used as a reference. Primers used are listed in Table 1.
Kim Y.J., Lee J.Y., Kim H.J., Kim D.H., Lee T.H., Kang M.S, & Park W. (2018). Anti-Inflammatory Effects of Angelica sinensis (Oliv.) Diels Water Extract on RAW 264.7 Induced with Lipopolysaccharide. Nutrients, 10(5), 647.
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Publication 2018
Actins Apoptosis Cells DDIT3 protein, human Electrophoresis Janus Kinase 2 Nitric Oxide Synthase Type II Oligonucleotide Primers Proto-Oncogene Proteins c-fos PTGS2 protein, human Real-Time Polymerase Chain Reaction RNA, Messenger STAT1 protein, human STAT3 Protein
5
Pentylenetetrazol-Induced c-Fos Expression
Cited 6 times
The animals were subjected to intraperitoneal injections of PTZ (50 mg/kg) or saline and sacrificed at specific time points. All animals were anesthetized with ketamine (60 mg/kg) and xylazine (15 mg/kg) intraperitoneally and subsequently decapitated. The right hemisphere was stored −80°C and later used for molecular biology procedures. The left hemisphere was placed in Eppendorf tubes (30 mL) containing 4% paraformaldehyde in PBS 0.01 M, pH 7.2, at 4°C for 5 days and then dried for 2 or more days in 30% sucrose in PBS 0.01 M pH 7.2 at 4°C for subsequent immunohistochemical analysis for c-Fos.
After fixation and dehydration the left hemispheres were sectioned in a cryostat in coronal sections of 30 µm thickness. The sections were processed for the immunohistochemical detection of c-Fos protein using a conventional avidin–biotin–immunoperoxidase technique to localize an antiserum raised against a synthetic N-terminal fragment of human Fos protein (rabbit polyclonal ab-5, Calbiochem). Briefly, free-floating sections were pretreated with hydrogen peroxidase for 10 min. Sections were treated with normal goat serum (1:100) and 0.3% Triton X-100 for 2 h and incubated with the primary antiserum at a dilution 1:5000 in KPBS at room temperature for 24 h. Subsequently, the sections were incubated with a secondary antibody (goat anti-rabbit IgG 1:200—Vector) for 2 h at room temperature and treated with avidin–biotin complex (Vector 1:100) for 90 min. Sections were submitted to nickel-intensified diaminobenzidine reaction. Between steps, the sections were rinsed in KPBS (pH 6.8) 0.05 M. The tissue was agitated on a rotator between each incubation and rinse step. Sections were mounted on gelatin-coated slides, dried, dehydrated, and coverslipped. To avoid eventual bias, at least one animal from each group was included in every staining batch. Four sections for each region (cingulate gyrus, piriform and primary motor cortex) were mounted on slides for histological evaluation. Histological counting of cells expressing c-Fos protein in the brain regions of interest was performed using the ImageJ® 1.45 s (National Institutes of Health USA) (Carnevali et al., 2004 (link)).
The number of c-Fos-positive cell nuclei within each area was counted in four consecutive sections per animal and the average of them was expressed as number of c-Fos-positive cells × 10−5/µm3. Stereological methods were not employed in this study due to potential bias associated with counts generated in this manner, such as uncertainties as to the extent to which antiserum penetrates through the thickness of the tissue sections and difficulties in defining the boundaries of the several cell groups of interest. Moreover, our interest was to make only relative comparisons of the strength of Fos induction as a function of the treatment status (Li and Sawchenko, 1998 (link); Medeiros et al., 2003 (link)).
Barros V.N., Mundim M., Galindo L.T., Bittencourt S., Porcionatto M, & Mello L.E. (2015). The pattern of c-Fos expression and its refractory period in the brain of rats and monkeys. Frontiers in Cellular Neuroscience, 9, 72.
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Publication 2015
Animals anti-IgG Avidin Biotin Brain Cell Nucleus Cloning Vectors Gelatins Goat Gyrus Cinguli Hydrogen Immune Sera Immunoglobulins Immunohistochemistry Immunoperoxidase Techniques Injections, Intraperitoneal Interest Groups Ketamine Motor Cortex, Primary Nickel paraform Peroxidase Proto-Oncogene Proteins c-fos Rabbits Saline Solution Serum Sucrose Technique, Dilution Tissues Triton X-100 Xylazine

Most recents protocols related to «Proto-Oncogene Proteins c-fos»

1
Validating Neuronal Activation by DREADDs
Although previous studies have validated the use of the CAV2-PRS construct using a range of actuators, including excitatory and inhibitory opsins, as well as potassium channels (Howorth et al., 2009a (link); Howorth et al., 2009b (link); Hickey et al., 2014 (link); Li et al., 2016 (link)), we used a separate cohort of rats to verify the functional effect of our DREADDs construct in vivo by quantifying changes in the expression of c-Fos, a recognized marker of neuronal activation, upon administration of DCZ. In order to obtain a high baseline of c-Fos activation, rats underwent a stress procedure. As shown in Figure 4—figure supplement 1B, rats were administered i.p. with either vehicle or DCZ (0.1 mg/kg) 45 min before being placed in a Plexiglas shock chamber equipped with stainless steel rods on the floor and a circuit generator connected to a scrambler and a timing unit. Rats received five shocks (0.5 s, 0.8 mA) randomly interspersed over 10 min (stress condition). As a control of c-Fos activation, we included in the experimental design animals administered with vehicle, but left untouched in their home cage (no stress condition). Rats were perfused 90 min after the procedure and coronal slices collected as described in the Histology section. For hM4Di/c-Fos colocalization analysis, sections were taken (see Figure 4—figure supplement 1—source data 1) from antero-posterior levels of the LC from –9.50 to –10.0 mm from Bregma (vehicle/no stress, n=2; vehicle-stress, n=4; DCZ-stress, n=4). Quantification of the percentage of LC hM4Di-positive cells (mCherry, red) that co-express c-Fos (Alexa 488, green) was performed by a trained observer blind to the experimental conditions.
Cerpa J.C., Piccin A., Dehove M., Lavigne M., Kremer E.J., Wolff M., Parkes S.L, & Coutureau E. (2023). Inhibition of noradrenergic signalling in rodent orbitofrontal cortex impairs the updating of goal-directed actions. eLife, 12, e81623.
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Publication 2023
Animals, Laboratory CAV2 protein, human Cells Dietary Supplements Neurons Opsins Plexiglas Potassium Channel Proto-Oncogene Proteins c-fos Psychological Inhibition Rattus norvegicus Rod Photoreceptors Shock Stainless Steel Stress Disorders, Traumatic Visually Impaired Persons
2
Quantifying Amygdalar Neuronal Activity
To evaluate the activity of the neurons in the amygdala, the cfos expression on neurons was calculated in the amygdala. Mice were sacrificed 90 min after fear conditioning. Brian slices (thickness of each slice was 40 μm) separated by 240 μm were immunostained with rabbit anti c-Fos antibody (1:500, 2250S, CST) followed by Alexa Flour 555 conjugated anti-rabbit IgG (1:2000, 4413S, CST) and DAPI (1:5000, CAS: 28718-90-3, Sigma®). Images were acquired using Leica upright fluorescence microscope (DM6B) using 20× objective and a pixel size of 72 nm. The boundaries of subregions of the amygdala were based on the mouse brain atlas. The c-Fos+ neurons were counted by ImageJ software.
Xuan S.M., Su Y.W., Liang Y.M., Gao Z.J., Liu C.Y., Fan B.F., Shi Y.W., Wang X.G, & Zhao H. (2023). mGluR5 in amygdala modulates fear memory generalization. Frontiers in Behavioral Neuroscience, 17, 1072642.
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Publication 2023
Amygdaloid Body anti-c antibody anti-IgG Brain DAPI Fear of disease Flour Mice, Laboratory Microscopy, Fluorescence Neurons Proto-Oncogene Proteins c-fos Rabbits
3
Mapping Psilocybin-Activated Neural Ensembles
Experiments were designed to address two goals: mapping the brain-wide distribution of c-Fos and, in the same mice, establishing the feasibility of controlling neural ensembles activated by psilocybin using the TRAP2 mouse line. In TRAP2 mice (Fos::c-Fos-2A-iCreERT2 knock-in; RRID:IMSR_JAX:030323), c-Fos and iCreERT2 are expressed in cells with Fos promoter activity and separated via ribosomal skipping of the 2A coding sequence during translation. Injection of 4-hydroxytamoxifen (4-OHT) activates iCreERT2, enabling translocation from the cytosol to the nucleus for site-specific recombination. TRAP2 mice were crossed to an Ai14 reporter line (Rosa-CAG-LSL::tdTomato; RRID:IMSR_JAX:007914) (Allen et al., 2017 (link)) for visualization of activated neurons via conditional tdTomato expression. Prior to the TRAPing timepoint, mice were naïve to experiments and treatments.
Twenty-five male and 8 female TRAP2;Ai14 mice (12–18 weeks of age) were bred in house to C57BL6/J wild type mice to give littermates of TRAP2+/− or TRAP−/−, and Ai14+/+, Ai14+/− or Ai14−/− genotypes (Transnetyx; Cordova, TN); a subset of mice (16 males and 6 females, TRAP+/−;Ai14+) was used to assess the feasibility of genetically labeling psilocybin-activated neural populations. Animals were maintained in age- and sex-matched groups of 2–5 littermates and housed in an 12:12 light-dark cycled SPF facility and appeared to be in good health; male mice weighed 31.4 ± 0.7 g (mean ± SEM) and females weighed 23 ± 0.9 g. Home cages were sterile ventilated cages by Innovive (San Diego, CA) offering irradiated 1/8” corn cob bedding (The Andersons, Inc.) with ad libitum access to pre-filled acidified water bottles (Innovive) and irradiated 18% protein rodent diet (Teklad Global). All animal behavioral procedures comply with the ARRIVE guidelines (Sert et al., 2020 (link)), the Guide for the Care and Use of Laboratory Animals (Council, 2011), and were approved by the Stanford University Institutional Animal Care and Use Committee. The animal care and use program is fully accredited by AAALAC, International and holds an Assurance with OLAW.
Rijsketic D.R., Casey A.B., Barbosa D.A., Zhang X., Hietamies T.M., Ramirez-Ovalle G., Pomrenze M., Halpern C.H., Williams L.M., Malenka R.C, & Heifets B.D. (2023). UNRAVELing the synergistic effects of psilocybin and environment on brain-wide immediate early gene expression in mice. bioRxiv.
Publication Preprint 2023
afimoxifene Animals Animals, Laboratory Behavior Therapy Brain Cell Nucleus Cells Cytosol Diet Females Genotype hydroxytamoxifen Institutional Animal Care and Use Committees Maize Males Mice, House Nervousness Neurons Open Reading Frames Population Group Proteins Proto-Oncogene Proteins c-fos Psilocybin Recombination, Genetic Ribosomes Rodent Rosa Sterility, Reproductive tdTomato Translocation, Chromosomal
4
Psilocybin-Induced Changes in Regional Co-Activity
To examine potential functional relationships among clusters representing validated effects of psilocybin on the c-Fos+ cell density, we calculated the inter-mouse Pearson correlation of c-Fos+ cell densities between each pair of valid clusters, generating a 27 × 27 correlation matrix for each condition. To note, this procedure of calculating activity correlations between regions resembles fMRI methods to determine functional connectivity. Here we refer to such correlations as “co-activity” to avoid confusion with “connectivity” in the fMRI literature. To examine changes in co-activity, we subtracted the saline correlations from those of psilocybin. Correlation matrices were calculated in R (version 4.1.3) using the psych package (version 2.2.3) and were visualized using the corrplot package (version 0.92).
Hierarchical clustering was used to delineate modules of co-active clusters in each correlation matrix. The correlation coefficient between each cluster pair was converted to the complete Euclidean distance for clustering. The derived dendrograms were trimmed at different tree heights (between 30–100% of the maximal tree height), to compare the total number of modules across hierarchical levels. A reliable difference in modularity should be consistent and robust between groups across different tree-cutting thresholds. We chose to highlight modularity results cut at 60% of the maximum tree height, which is near the elbow of the height by modularity curve. Hierarchical clustering was conducted using the built-in hclust function in the R stats package and was visualized using the heatmap.2 function in gplots (version 3.1.3).
Inspired by work from Kimbrough et al. (Kimbrough et al., 2020 (link), 2021 (link)), looking at inter- and intra-module characteristics of between-region co-activity as defined by c-Fos+ cell counts, we used the same graph theory approach to examine psilocybin-induced changes in regional centrality metrics, including the Z-scored version of within-module degree (WMDz) and the participation coefficient (PC). The WMDz represents the relative importance of a cluster within its own module, which corresponds to intra-module connectivity. The PC measures the extent a cluster correlates with multiple modules, which corresponds to the inter-module connectivity (Guimerà & Nunes Amaral, 2005 (link)). As recommended elsewhere (Kimbrough et al., 2020 (link), 2021 (link)) we thresholded the co-activity correlograms by removing co-activity edges weaker than r = 0.75 and excluded negative co-activity edges following conventions as no consensus was reached regarding how to handle them in graph theory analysis (Hallquist & Hillary, 2018 (link)). Graph theory analysis was conducted using the Brain Connectivity Toolbox (Rubinov & Sporns, 2010 (link)) in Matlab R2020b.
Rijsketic D.R., Casey A.B., Barbosa D.A., Zhang X., Hietamies T.M., Ramirez-Ovalle G., Pomrenze M., Halpern C.H., Williams L.M., Malenka R.C, & Heifets B.D. (2023). UNRAVELing the synergistic effects of psilocybin and environment on brain-wide immediate early gene expression in mice. bioRxiv.
Publication Preprint 2023
Brain Conferences Elbow fMRI Mus Proto-Oncogene Proteins c-fos Psilocybin Saline Solution Trees
5
Biomarker Analysis of Bladder Function
In the biochemical analyses, the following biomarkers were analyzed. C-Fos (c-Fos; MyBioSource, MBS729725) expression was measured in the central micturition areas: medial preoptic area (MPA), the ventrolateral periaqueductal gray (vlPAG), and pontine micturition center (PMC) (as described in detail in Section 4.7.1 below). The levels of Vesicular acetylcholine transporter (VAChT; LifeSpan BioSciences, CN LS-F12924-1) and Rho Kinase (ROCK1; LifeSpan BioSciences, LS-F32208) were assessed in bladder detrusor muscle, while the concentrations of nerve growth factor (NGF; LifeSpan BioSciences, CN LS-F25946-1) and brain-derived neurotrophic factor (BDNF; PROMEGA, CN G7610) were analyzed in urine. As for the bladder urothelium, the following markers were measured: tumor necrosis factor α (TNF- α, LifeSpan BioSciences; LS-F5193), interleukin 1β (IL-1β, Cloud-Clone; SEA563Ra), interleukin 6 (IL-6, LifeSpan BioSciences; LS-F25921-1), Calcitonin Gene-Related Peptide (CGRP; Biomatik, CN EKU02858), ATP Citrate Lyase (ATP; LifeSpan BioSciences, LS-F10730), corticotropin-releasing factor (CRF; Alpco, Salem, NH, USA, CN 48-CRFMS-E01), Organic Cation Transporter 3 (OCT3; antibodies-online, CN ABIN6227163), and Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1; LSBio, LS-F36019). Finally, the respective values of TNF-α, IL-1β, IL-6, interleukin 10 (IL-10, LifeSpan BioSciences; LS-F5081), CRF, BDNF, and NGF were measured in the hippocampus (as described in Section 4.7.2 below). All measurements were carried out according to the manufacturers’ instructions. Each sample was measured in duplicate. The results are presented in pg/mL.
Zapała Ł., Niemczyk G., Zapała P., Wdowiak A., Bojar I., Kluz T., Szopa A., Serefko A., Radziszewski P, & Wróbel A. (2023). The Cannabinoid Ligand Arachidonyl-2′-Chloroethylamide (ACEA) Ameliorates Depressive and Overactive Bladder Symptoms in a Corticosterone-Induced Female Wistar Rat Model. International Journal of Molecular Sciences, 24(4), 3820.
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Publication 2023
Acetylcholine Transporters, Vesicular Antibodies ATP Citrate (pro-S)-Lyase Biological Markers Bladder Detrusor Muscle Calcitonin Gene-Related Peptide Central Gray Substance of Midbrain Clone Cells Corticotropin-Releasing Hormone IL1B protein, human IL10 protein, human Interleukin-1 beta Membrane Transport Proteins Nerve Growth Factor Neurotrophic Factor, Brain-Derived Pons POU5F1 protein, human Preoptic Areas, Medial Promega Proto-Oncogene Proteins c-fos rho-Associated Kinases ROCK1 protein, human Seahorses TNF protein, human Transient Receptor Potential Channels Tumor Necrosis Factors Urinary Bladder Urination Urine Urothelium

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More about "Proto-Oncogene Proteins c-fos"

Explore the versatile role of Proto-Oncogene Proteins c-fos, a critical regulator of cellular proliferation and differentiation.
As a member of the immediate early gene family, the c-fos proto-oncogene and its protein product, c-Fos, play a pivotal part in the transcription factor complex AP-1, which mediates cellular responses to a variety of extracellular stimuli.
Discover the latest protocols and optimize your c-fos research with the help of PubCompare.ai, a powerful AI-driven tool that allows you to identify the most efficient protocols and products.
Delve deeper into the world of c-fos and its related terms, such as C-Fos, β-actin, FBS (Fetal Bovine Serum), C-Jun, PVDF membranes, P-ERK, and NFATc1.
Understand how dysregulation of c-fos expression has been implicated in the development of certain cancers, making it a crucial target for biological research and therapeutic interventions.
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