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RETN protein, human

RETN (resistin) is a cysteine-rich adipose-specific secretory factor that may play a role in insulin resistance and glucose homeostasis.
It is expressed primarily in adipose tissue and has been implicated in the pathogenesis of obesity and type 2 diabetes.
RETN protein research can be optimized using PubCompare.ai, an AI-driven platform that enhances reproducibility and accuracy.
This tool helps researchers easily locate protocols from literature, pre-prints, and patents, and leverages AI-driven comparisons to identify the best protocols and products, streamlining the research process and achieving more reliable results.

Most cited protocols related to «RETN protein, human»

1
Optimizing SOMAmer-based Analyte Detection
Cited 20 times
SOMAmers were grouped into 2 mixes according to the relative abundance of their cognate analytes. SOMAmers consigned to the low-abundance group were those that recognize IL-8, MMP-7, resistin, and tPA. SOMAmers consigned to the high-abundance group were those that recognize Lipocalin-2, MCP-1, MIP-4 (PARC), MMP-9, and RANTES. Stock concentrations were 4 nM in each SOMAmer, and the final concentration of each SOMAmer was 0.5 nM. SOMAmer stock mixes were diluted 4-fold in SB17 buffer, heated to 95°C for 5 min and cooled to 37°C over a 15 minute period prior to use. This denaturation-renaturation cycle is intended to normalize SOMAmer conformer distributions and thus ensure reproducible SOMAmer activity in spite of variable histories. Streptavidin plates were washed twice with 150 µL buffer PB1 prior to use.
Kraemer S., Vaught J.D., Bock C., Gold L., Katilius E., Keeney T.R., Kim N., Saccomano N.A., Wilcox S.K., Zichi D, & Sanders G.M. (2011). From SOMAmer-Based Biomarker Discovery to Diagnostic and Clinical Applications: A SOMAmer-Based, Streamlined Multiplex Proteomic Assay. PLoS ONE, 6(10), e26332.
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Publication 2011
Buffers CCL2 protein, human CCL5 protein, human CCL18 protein, human Lipocalin-2 MMP7 protein, human MMP9 protein, human RETN protein, human Streptavidin
2
Mortality Prediction in Type 2 Diabetes
Cited 5 times
One thousand and twenty-eight patients with type 2 diabetes (ADA 2003 criteria) were consecutively recruited from November 1th 2000 to September 30th 2005 at the Endocrine Unit of IRCCS “Casa Sollievo della Sofferenza” in San Giovanni Rotondo, for a study having all-cause mortality as the end-point (Figure S1). The only exclusion criterion was the presence of poor life expectancy due to non diabetes-related disorders. This cohort was followed until 2010 by obtaining information on the participants’ vital status by direct contact with patients and/or their relatives or by queries to the registry offices of the cities of residence. Such information was available in 838 individuals whose data were therefore analyzed in the present study. One hundred and three of the GMS participants are also participants of the GHS-prospective design (Figure S1).
Serum resistin was measured in 779 (93%) participants.
Menzaghi C., Bacci S., Salvemini L., Mendonca C., Palladino G., Fontana A., De Bonis C., Marucci A., Goheen E., Prudente S., Morini E., Rizza S., Kanagaki A., Fini G., Mangiacotti D., Federici M., De Cosmo S., Pellegrini F., Doria A, & Trischitta V. (2013). Serum Resistin, Cardiovascular Disease and All-Cause Mortality in Patients with Type 2 Diabetes. PLoS ONE, 8(6), e64729.
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Publication 2013
Chaperone-Mediated Autophagy Diabetes Mellitus Diabetes Mellitus, Non-Insulin-Dependent Patients RETN protein, human Serum System, Endocrine
3
Clinical Biomarkers in Sepsis Pathophysiology
Cited 5 times
The patients in the sepsis and non-sepsis groups were compared by age, sex, body mass index (BMI), pre-existing diabetes mellitus, and severity of disease using the APACHE II score [18 (link)] at admittance.
ICU treatment such as volume therapy, vasopressor infusions, demand of ventilation and ventilation hours, antibiotic and antimycotic therapy, renal replacement therapy, and nutrition were recorded, alongside a large number of laboratory parameters that were routinely assessed during ICU treatment. Resistin serum concentrations were analysed using a quantitative sandwich immunoassay (ELISA; BioVendor, LLC, Candler, NC, USA). IL-6, IL-10, TNF-alpha (all Siemens Healthcare, Erlangen, Germany), and procalcitonin (Kryptor, B.R.A.H.M.S. Diagnostica, Henningsdorf, Germany) were measured by commercial chemiluninescence assays, following manufacturers' instructions.
Koch A., Gressner O.A., Sanson E., Tacke F, & Trautwein C. (2009). Serum resistin levels in critically ill patients are associated with inflammation, organ dysfunction and metabolism and may predict survival of non-septic patients. Critical Care, 13(3), R95.
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Publication 2009
Antibiotics Biological Assay Enzyme-Linked Immunosorbent Assay IL10 protein, human Immunoassay Index, Body Mass Patients Procalcitonin Renal Replacement Therapy RETN protein, human Septicemia Serum States, Prediabetic Tumor Necrosis Factor-alpha Vasoconstrictor Agents
4
Measuring Biomarkers for Rheumatoid Arthritis
Cited 4 times
De-identified serum specimens were tested in the Clinical Laboratory Improvement Amendment-certified clinical laboratory of Crescendo Bioscience (South San Francisco, CA, USA). A multiplexed, sandwich immunoassay (Mesoscale Discovery, Rockville, MD, USA) was used to measure concentrations of the 12 MBDA protein biomarkers: vascular cell adhesion molecule 1, epidermal growth factor, VEGF alpha, IL-6, TNF receptor-I, MMP-1, MMP-3, YKL-40, leptin, resistin, serum amyloid A and CRP. Concentration values were combined using a previously validated algorithm that generates an integer score on a scale of 1–100 [2 ]. Samples were tested from 2012 to 2017, using the same immunoassay instruments, reagents and algorithm as those used for the Vectra DA commercial test manufactured by Crescendo Bioscience.
Curtis J.R., Flake DD I.I., Weinblatt M.E., Shadick N.A., Østergaard M., Hetland M.L., Brahe C.H., Hwang Y.G., Furst D.E., Strand V., Etzel C.J., Pappas D.A., Wang X., Hwang C.C., Sasso E.H., Gutin A., Hitraya E, & Lanchbury J.S. (2018). Adjustment of the multi-biomarker disease activity score to account for age, sex and adiposity in patients with rheumatoid arthritis. Rheumatology (Oxford, England), 58(5), 874-883.
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Publication 2018
3-benzoyl dopamine Biological Markers CHI3L1 protein, human Epidermal growth factor Immunoassay Interstitial Collagenase Leptin liquid crystal polymer Matrix Metalloproteinase 3 Proteins RETN protein, human Serum Serum Amyloid A Protein Tumor Necrosis Factor Receptor Vascular Cell Adhesion Molecule-1 Vascular Endothelial Growth Factors
5
Adipokine Regulation of Chondrocyte Metabolism
Cited 4 times
T/C-28a2 cell line and human OA chondrocytes, at the first passage, were plated in 6-well dishes at a starting density of 6 × 106 cells/well until they became confluent. Human recombinant visfatin (Sigma–Aldrich, Milan, Italy) and human recombinant resistin (BioVendor, Rome, Italy) were first dissolved in phosphate buffered saline (PBS) (Euroclone, Milan, Italy), according to the manufacturer’s instructions, and then they were diluted in the culture medium immediately before the treatment to reach the final concentration required.
The cells were treated for 24 h with visfatin at concentration of 5 μg/mL and 10 μg/mL or resistin 50 ng/mL and 100 ng/mL. The concentrations of the adipokines used in our in vitro study were selected according to those used by other authors [12 (link),39 (link)]; the final concentrations were chosen based on the best results obtained in terms of viability.
After the treatment, the media were removed, centrifuged, and stored at −80 °C; the cells were immediately processed to carry out cell viability assay, flow cytometry analysis, and quantitative real-time PCR.
Afterwards, the cells were pre-incubated for 2 h with 1 μM BAY 11-7082 (NF-κB inhibitor, IKKα/β, Sigma–Aldrich, Milan, Italy) and then treated 24 h with the tested concentrations of visfatin and resistin. Subsequently, the gene expression of MMP-1, MMP-13, and Col2a1 was evaluated.
Cheleschi S., Giordano N., Volpi N., Tenti S., Gallo I., Di Meglio M., Giannotti S, & Fioravanti A. (2018). A Complex Relationship between Visfatin and Resistin and microRNA: An In Vitro Study on Human Chondrocyte Cultures. International Journal of Molecular Sciences, 19(12), 3909.
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Publication 2018
Adipokines BAY 11-7082 Biological Assay Cells Cell Survival Chondrocyte Conserved Helix-Loop-Helix Ubiquitous Kinase Culture Media Flow Cytometry Gene Expression Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal I-kappa B Proteins Interstitial Collagenase MMP13 protein, human nicotinamide phosphoribosyltransferase, human Phosphates Real-Time Polymerase Chain Reaction RETN protein, human Saline Solution T-Lymphocyte

Most recents protocols related to «RETN protein, human»

1
Blood Biomarker Profiling in Mice
Mice at the fed state or fasted for 12 h were sacrificed, and whole blood was collected from retrobulbar venous plexus and centrifuged for 10 min at 12,000 × g to obtain plasma. Plasma glucose, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and total triglycerides were measured using commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). ELISA kits (Sinoukbio, Beijing, China) were used to measure plasma insulin, C-peptide, non-esterified fatty acid (NEFA), leptin, IL (interleukin) 4, IL6, IL10, resistin, interferon γ (IFNγ) and monocyte chemotactic protein-1 (MCP1) following the manufacturer’s instruction.
Zhang X., Wang L., Wang Y., He L., Xu D., Yan E., Guo J., Ma C., Zhang P, & Yin J. (2023). Lack of adipocyte IP3R1 reduces diet-induced obesity and greatly improves whole-body glucose homeostasis. Cell Death Discovery, 9, 87.
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Publication 2023
BLOOD C-Peptide Cholesterol Enzyme-Linked Immunosorbent Assay Fatty Acids, Esterified Glucose High Density Lipoproteins IL10 protein, human Insulin Interferon Type II Interleukin-4 Leptin Low-Density Lipoproteins Monocyte Chemoattractant Protein-1 Mus Plasma RETN protein, human Triglycerides Veins Very Low Density Lipoprotein
2
Serum Lipid and Adipocytokine Profiling in GHD
After an overnight fast, blood samples were collected from both the GHD and control groups to analyze serum lipid levels, including triglycerides (TG), total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and adipocytokines including leptin, resistin and ghrelin. The lipid profile was analyzed using routine laboratory methods. Serum samples were stored at -80 °C. Leptin was measured with a kit from Labsystems Diagnostics Oy (Vantaa, Finland), and resistin and ghrelin with kits from USCN Life Science Inc (Wuhan, PRC) using the enzyme-linked immunosorbent assay (ELISA) method. The sensitivities of the ELISA kits (detection limits) were 0.04 ng/mL, 0.2 ng/mL, and 21 pg/mL for leptin (Cat. No. 140101/A), resistin (Cat. No. L140718502), and ghrelin (Cat. No. L140718468), respectively. Intra-assay variability was 3.5-13.3%, <5.8% and 3.3-10.0% for leptin, resistin, and ghrelin, respectively. Inter-assay variability was 10.2%, 14.8% and 14.7% for these markers, respectively.
Saygılı S., Kocaağa M., Kaya G., Şükür M., Baş F., Poyrazoğlu Ş., Bundak R, & Darendeliler F. (2023). Increased Carotid Intima-media Thickness and Its Association with Carbohydrate Metabolism and Adipocytokines in Children Treated with Recombinant Growth Hormone. Journal of Clinical Research in Pediatric Endocrinology, 15(1), 69-80.
Publication 2023
Adipokines Biological Assay BLOOD Cholesterol Diagnosis Enzyme-Linked Immunosorbent Assay GHRL protein, human High Density Lipoprotein Cholesterol Hypersensitivity Leptin Lipids Low-Density Lipoproteins RETN protein, human Serum Triglycerides
3
Liver Metabolism and Antioxidant Bioassay
Liver tissues were homogenized with physiological saline, centrifuged twice at 3500 r/min for 10 min each time, and the protein concentrations were determined using a bicinchoninic acid protein assay kit (#23227; Thermo Fisher Scientific). The levels of high-density lipoprotein cholesterol (HDL-C, #KT2827-A), total cholesterol (#KT30043-A), low-density lipoprotein cholesterol (LDL-C, #KT2797-A), aspartate aminotransferase (AST, #KT2856-A), glycosylated hemoglobin A1c (GHbA1c, #KT2511-A), insulin (#KT2579-A), adiponectin (#KT2547-A), resistin (#KT2552-A), ROS (#KT9261-A), malondialdehyde (MDA, #KT9264-A), glutathione peroxidase (GSH-Px, #KT2755-A), and catalase (CAT, #KT2833-A) were determined using enzyme-linked immunosorbent assay kits (Jiangsu Kete Biotechnology Co., Ltd., Jiangsu, China) according to the manufacturer’s instruction manuals.
Li L., Zhai S., Wang R., Kong F., Yang A., Wang C., Yu H., Li Y, & Wang D. (2023). Anti-Obesity Effect of Auricularia delicate Involves Intestinal-Microbiota-Mediated Oxidative Stress Regulation in High-Fat-Diet-Fed Mice. Nutrients, 15(4), 872.
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Publication 2023
ADIPOQ protein, human Aspartate Transaminase bicinchoninic acid Biological Assay Catalase Cholesterol Cholesterol, beta-Lipoprotein Enzyme-Linked Immunosorbent Assay Glycated Hemoglobin A1c Hypercholesterolemia Insulin Lipoproteins Liver Malondialdehyde Peroxidase, Glutathione physiology Proteins RETN protein, human Saline Solution Tissues
4
Ileum tissue total RNA extraction and qPCR analysis
Total RNA from the ileum tissues was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s procedures. Real-time quantitative PCR was performed using the One Step SYBR PrimeScript plus RT-PCR Kit (TaKaRa Biotechnology Company Limited, Dalian, China) in the 7500 real-time PCR system (Applied Biosystems, Waltham, MA, USA). The expression of Gapdh mRNA served as an internal control, and the results were analyzed using the ΔΔCT method. The qPCR primers were synthesized and purified by GENERAY Biotechnology (Shanghai, China), and the following primers were used: for MuRF-1, 5′-GATGGAAACGCTATGGAGAACC-3′ and 5′-ACGGAAACGACCTCCAGACAT-3′; for krüppel-like factor 4 (klf4), 5′-AGGAACTCTCTCACATGAAGCG-3′ and 5′-GGTCGTTGAACTCCTCGGTC-3′; for resistin-like molecule-beta (Relm-β), 5′-CCATTTCCTGAGCTTTCTGG-3′ and 5′-AGCACATCCAGTGACAACCA-3′; and for trefoil factor 3 (Tff3), 5′-CAGATTACGTTGGCCTGTCTCC-3′ and 5′-ATGCTTGCTACCCTTGGACCAC-3′.
Xu Y., Duan J., Wang D., Liu J., Chen X., Qin X.Y, & Yu W. (2023). Akkermansia muciniphila Alleviates Persistent Inflammation, Immunosuppression, and Catabolism Syndrome in Mice. Metabolites, 13(2), 194.
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Publication 2023
GAPDH protein, human Ileum KLF4 protein, human Oligonucleotide Primers Real-Time Polymerase Chain Reaction RETN protein, human Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger TFF3 protein, human Tissues TRIM63 protein, human trizol
5
Measurement of Adipokines in Rat Serum
According to the manufacturer’s instructions, serum levels of leptin, adiponectin and resistin were measured using a rat insulin ELISA kit from Ray Biotech (3607 Parkway Lane, IL, USA). <10.0% for the intra-assay coefficient of variance and <12.0% for the inter-assay coefficient of variation. Leptin levels were indicated in pg/mL, whilst adiponectin and resistin results were expressed in ng/mL.
Rebecca Roy J., Janaki C.S., Jayaraman S., Periyasamy V., Balaji T., Vijayamalathi M., Veeraraghavan V.P., Krishnamoorthy K, & Prasad M. (2023). Carica Papaya Reduces High Fat Diet and Streptozotocin-Induced Development of Inflammation in Adipocyte via IL-1β/IL-6/TNF-α Mediated Signaling Mechanisms in Type-2 Diabetic Rats. Current Issues in Molecular Biology, 45(2), 852-884.
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Publication 2023
ADIPOQ protein, human Biological Assay Enzyme-Linked Immunosorbent Assay Insulin Leptin RETN protein, human Serum

Top products related to «RETN protein, human»

1
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
2
Resistin by R&D Systems
Sourced in United States, United Kingdom
Resistin is a protein that is secreted by adipose tissue and has been associated with insulin resistance. It is a member of the resistin-like molecule (RELM) family of proteins. Resistin is involved in glucose and lipid metabolism, but its exact physiological functions are still being investigated.
3
Milliplex map kit by Merck Group
Sourced in United States, Germany, Canada, France, Morocco, Australia, United Kingdom
The Milliplex MAP Kit is a multiplex assay platform developed by Merck Group. The kit enables the simultaneous detection and measurement of multiple analytes in a single sample. The core function of the Milliplex MAP Kit is to provide a versatile and efficient tool for researchers to conduct multiplexed biomarker analysis.
4
Recombinant human resistin by Thermo Fisher Scientific
Sourced in United States
Recombinant human resistin is a protein produced through recombinant DNA technology. It is a member of the resistin-like molecule family and is involved in the regulation of glucose and lipid metabolism.
5
Resistin by Merck Group
Sourced in United States, United Kingdom
Resistin is a laboratory equipment product designed to measure the concentration of resistin, a protein involved in insulin resistance. Resistin is a hormone secreted by adipose tissue and plays a role in glucose and lipid metabolism. The product provides a tool for researchers and clinicians to quantify resistin levels, which may be useful in the study of metabolic disorders such as type 2 diabetes.
6
Human resistin quantikine elisa kit by R&D Systems
Sourced in United States
The Human Resistin Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human resistin levels in cell culture supernates, serum, and plasma. The kit includes a microplate pre-coated with an antibody specific for human resistin, and use of this kit provides a precise quantification of human resistin concentrations.
7
Resistin by BioVendor
Sourced in United States, Czechia
Resistin is a protein that plays a role in insulin resistance and glucose homeostasis. It is secreted primarily by adipose tissue and is involved in the regulation of glucose and lipid metabolism. Resistin can be used in research applications to study its functions and interactions.
8
Resistin by Thermo Fisher Scientific
Sourced in United States
Resistin is a lab equipment product manufactured by Thermo Fisher Scientific. It is a protein that is primarily used for research purposes in the field of molecular biology. Resistin functions as an adipokine, which is a hormone secreted by adipose tissue. The core function of Resistin is to assist researchers in understanding the role of adipokines in various biological processes.
9
Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
10
Recombinant human resistin by R&D Systems
Sourced in United States
Recombinant human resistin is a protein produced using recombinant DNA technology. It is a member of the resistin-like molecule family and is involved in the regulation of glucose and lipid metabolism.

More about "RETN protein, human"

Resistin, also known as RETN, is a cysteine-rich adipose-specific secretory factor that plays a crucial role in insulin resistance and glucose homeostasis.
This adipokine is primarily expressed in adipose tissue and has been implicated in the pathogenesis of obesity and type 2 diabetes.
Optimizing RETN protein research can be achieved through the use of PubCompare.ai, an AI-driven platform that enhances reproducibility and accuracy.
This innovative tool helps researchers easily locate protocols from literature, pre-prints, and patents, and leverages AI-driven comparisons to identify the best protocols and products.
By streamlining the research process, PubCompare.ai enables researchers to achieve more reliable results.
To further explore RETN protein research, researchers can utilize various techniques and reagents, such as TRIzol reagent for RNA extraction, Milliplex Map Kits for multiplex analysis, and recombinant human resistin for in vitro studies.
The Human Resistin Quantikine ELISA Kit can also be employed for quantitative determination of resistin levels in biological fluids.
Additionally, Lipofectamine 2000 can be used for efficient transfection of RETN-related constructs into cell lines.
By incorporating these insights and tools, researchers can optimize their RETN protein research, leading to a better understanding of this adipokine's role in insulin resistance, obesity, and type 2 diabetes.
This knowledge can ultimately contribute to the development of targeted therapies and improved patient outcomes.