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RNA Polymerase III

RNA Polymerase III is a eukaryotic enzyme that transcribes small nuclear RNAs, transfer RNAs, and other short, untranslated RNAs.
It plays a crucial role in gene expression and cellular processes.
PubCompare.ai can help optimize your RNA Polymerase III research by identifying the most accurate and reproducible protocols from scientific literature, preprints, and patents.
Leverage our intelligent comparisons to select the best products and methodologies, ensuring your experiments deliver reliable, reproducible results and advance your research journey effeciently.

Most cited protocols related to «RNA Polymerase III»

1
Comprehensive Mouse Cell Surface Marker sgRNA Design
Cited 19 times
For a collection of mouse cell surface markers, as well as negative
control targets, we identified all sgRNA target sites preceding the NGG PAM
sequences on the plus and minus strands of DNA for all exons, including 25 nts
of flanking intronic sequence, as annotated in the Ensembl Genome Browser. To
all 20 nt sgRNA sequences we prepended a G to allow for proper transcription
initiation by RNA polymerase III (Supplementary Table 1). For the human cell surface markers,
we limited our design to coding sequence sgRNAs.
Doench J.G., Hartenian E., Graham D.B., Tothova Z., Hegde M., Smith I., Sullender M., Ebert B.L., Xavier R.J, & Root D.E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nature biotechnology, 32(12), 1262-1267.
Publication 2014
Cells Exons Genome Homo sapiens Introns Mus Open Reading Frames RNA Polymerase III
2
Engineered dCas9-Based Transcriptional Regulators for Yeast
Cited 15 times
dCas9 (endonuclease-deficient Cas9, with D10A and H841A
mutations
relative to the wild-type sequence of S. pyogenes Cas939 (link)) with an N-terminal SV40 nuclear
localization signal (NLS) was codon-optimized for expression in S. cerevisiae and cloned into a pRS314 backbone under
control of the pTPGI promoter.23 (link) The RNA-guided
transcription factors (crisprTFs) were built by fusing four repeats
of the minimal domain of the herpes simplex viral protein 16 (VP16)
to the C-terminus of dCas9 (dCas9_VP64). The crisprTF-expressing plasmid
was then integrated into the TRP1 locus of S. cerevisiae W303.
The reporter plasmids were built by cloning yeast-enhanced gfp under the control of the wild-type or modified pCYC1m
promoter into pRS406 using one-step Gibson assembly. The reporters
for the multiple-gRNA-binding-site experiment (Figure 4A) were built by cloning the corresponding number of binding
sites upstream of the pCYC1m promoter driving production of EBFP2. All reporters were integrated into the bla1 locus of the integrated crisprTF plasmid.
To build gRNA-expressing
plasmids, empty gRNA expressing vectors
were first made by cloning the pRPR1 promoter (an RNA-polymerase-III-dependent
promoter44 (link)), the gRNA handle (flanked by HindIII and Xho1 sites), and the RPR terminator into the
SacI and KpnI sites of either the pRS423 or pRS425
plasmid using one-step Gibson assembly. The specificity determinant
sequence (SDS) for each gRNA was then cloned into the HindIII site of these vectors by one-step Gibson assembly. Sequences
of the constructs used in this study are listed in Table S1, Supporting Information.
Farzadfard F., Perli S.D, & Lu T.K. (2013). Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synthetic Biology, 2(10), 604-613.
Publication 2013
Binding Sites Cloning Vectors Codon Endonuclease Plasmids Protein Domain RNA Polymerase III Saccharomyces cerevisiae Simian virus 40 Simplexvirus Streptococcus pyogenes tyrosinase-related protein-1 Vertebral Column
3
Genetic Manipulation of Neural Signaling Pathways
Cited 10 times
Cloning, mutagenesis, and deletion of DISC1 and Kal-7 were conducted as described in the prior publication25 (link). Wild-type, constitutively-active (Rac1-CA, G12V), and dominant-negative form of Rac1 (Rac1-DN, T17N) were from Missouri University of Science and Technology (Rolla, MO). H1-RNA polymerase III promoter-driven shRNA against DISC1 (#1 with strong effect and #2 with weaker effect), which also carry EGFP under the downstream of CMV promoter, were previously described25 (link). Cultured cells were transfected with plasmids by the use of LipofectAMINE2000 (Invitrogen). In primary neuron culture, 2μg of pSuper-Venus RNAi were transfected into 1.5 × 105 cells. For 293 cells, 2 μg of pRK/DISC1-HA expression construct and 2 μg of pSuper-Venus RNAi construct were transfected (at 90 % confluence in 18 mm dish). In standard analyses, primary neurons were transfected after 23 DIV and maintained for 1–6 days after transfection.
Hayashi-Takagi A., Takaki M., Graziane N., Seshadri S., Murdoch H., Dunlop A.J., Makino Y., Seshadri A.J., Ishizuka K., Srivastava D.P., Xie Z., Baraban J.M., Houslay M.D., Tomoda T., Brandon N.J., Kamiya A., Yan Z., Penzes P, & Sawa A. (2010). Disrupted-in-Schizophrenia-1 (DISC1) regulates spines of the glutamate synapse via Rac1. Nature neuroscience, 13(3), 327-332.
Publication 2010
Cells Cultured Cells Deletion Mutation Hyperostosis, Diffuse Idiopathic Skeletal Mutagenesis Neurons Plasmids RNA Interference RNA Polymerase III Short Hairpin RNA Transfection
4
Genetic Manipulation of Neural Signaling Pathways
Cited 10 times
Cloning, mutagenesis, and deletion of DISC1 and Kal-7 were conducted as described in the prior publication25 (link). Wild-type, constitutively-active (Rac1-CA, G12V), and dominant-negative form of Rac1 (Rac1-DN, T17N) were from Missouri University of Science and Technology (Rolla, MO). H1-RNA polymerase III promoter-driven shRNA against DISC1 (#1 with strong effect and #2 with weaker effect), which also carry EGFP under the downstream of CMV promoter, were previously described25 (link). Cultured cells were transfected with plasmids by the use of LipofectAMINE2000 (Invitrogen). In primary neuron culture, 2μg of pSuper-Venus RNAi were transfected into 1.5 × 105 cells. For 293 cells, 2 μg of pRK/DISC1-HA expression construct and 2 μg of pSuper-Venus RNAi construct were transfected (at 90 % confluence in 18 mm dish). In standard analyses, primary neurons were transfected after 23 DIV and maintained for 1–6 days after transfection.
Hayashi-Takagi A., Takaki M., Graziane N., Seshadri S., Murdoch H., Dunlop A.J., Makino Y., Seshadri A.J., Ishizuka K., Srivastava D.P., Xie Z., Baraban J.M., Houslay M.D., Tomoda T., Brandon N.J., Kamiya A., Yan Z., Penzes P, & Sawa A. (2010). Disrupted-in-Schizophrenia-1 (DISC1) regulates spines of the glutamate synapse via Rac1. Nature neuroscience, 13(3), 327-332.
Publication 2010
Cells Cultured Cells Deletion Mutation Hyperostosis, Diffuse Idiopathic Skeletal Mutagenesis Neurons Plasmids RNA Interference RNA Polymerase III Short Hairpin RNA Transfection
5
Construction and Characterization of HIV-1 Mutants
Cited 10 times
The full-length molecular HIV-1 clone LAI (29 (link)) was used to produce wild-type and mutant viruses. Nucleotide numbers presented here refer to the position on the genomic HIV-1 RNA transcript, with +1 being the capped G residue. The mutant proviral DNA sequences were PCR-amplified from cellular DNA with the 5′ Env primer tTA1-AD (+8269 to +8289) and the 3′ U5 primer CN1 (+9253 to +9283). The PCR fragments were digested with XhoI and BspEI, and cloned into the plasmid Blue-3′LTR (30 (link)). The XhoI–BglI fragments (1709 bp) of these plasmids were cloned into the wild-type LAI clone, resulting in the full-length mutant clones R1–R9.
The firefly luciferase expression vector pGL3 control (Promega) was used to construct the wild-type and mutant reporter-Nef target plasmids (pGL3-Nef). An approximately 250 bp Nef fragment (+8448 to +8698) was PCR amplified from the full-length molecular clones with the primers EW1 (5′-ACGTCTAGAATTCTGAGACGAGCTGAGCCAGCA-3′) and EW3 (5′-GACTCTAGACTGCAGGAGTGAATTAGCCCTTCCA-3′). The PCR product was digested with XbaI and cloned into the XbaI site located downstream of the luciferase gene in pGL3 control. The forward orientation of the insert was checked by sequence analysis.
To construct the m1–m4 mutants, base changes were introduced into pGL3-Nef by mutagenesis PCR (31 (link)). Mutagenic (m) primers EWmut1 (5′-ACAGCAGCTACCAATCCTGCTTGTGC -3′, mismatching nucleotide underlined; m1), EWmut2 (5′-CACAAGTAGGAATACAGCAGCTACCAACCTGCTTGTGC-3′; m2), EWmut3 (5′-CACAAGTAGTAATACAGCAG-3′; m3 and m4), and the general primers EW1 (Primer 1), EW2 (5′-TGAGGCCCGGTACCTGAGGTGTGACT-3′; primer 2), and EW3 (Primer 3) were used with the wild-type pGL3-Nef (m1, m2 and m4) or the R8 pGL3-Nef (m3) template. Briefly, PCR reactions were performed with primer M plus primer 3, and with primer 1 plus primer 2. The PCR products were purified, mixed and PCR amplified with primers 1 and 3 as described previously (31 (link)). The PCR fragments were digested with XbaI and cloned in the corresponding site of pGL3 control. All mutations were verified by sequence analysis.
The pRetro-SUPER-shNef vector, which expresses siRNA-Nef under control of the H1 RNA polymerase III promoter was digested with EcoRI and XhoI, and the 314 bp expression cassette was ligated into the EcoRI/XhoI sites of pBluescriptII (KS+) (Stratagene) to produce pBS-siRNA-Nef. Plasmid pRL-CMV (Promega) expresses renilla luciferase under control of the CMV promoter.
Westerhout E.M., Ooms M., Vink M., Das A.T, & Berkhout B. (2005). HIV-1 can escape from RNA interference by evolving an alternative structure in its RNA genome. Nucleic Acids Research, 33(2), 796-804.
Publication 2005
Cells Cloning Vectors Deoxyribonuclease EcoRI DNA Sequence Genes Genome HIV-1 Luciferases Luciferases, Firefly Luciferases, Renilla Mutagenesis Mutation Nucleotides Oligonucleotide Primers Paragangliomas 3 Plasmids Promega Proviruses RNA, Small Interfering RNA Polymerase III Sequence Analysis Virus

Most recents protocols related to «RNA Polymerase III»

1
Adenoviral Knockdown of FYN and FGF18 in NRCMs
The pSilencer 2.1-U6 expression vector was purchased from Ambion (Ambion, AM5762). The RNU6-1 RNA polymerase III promoter and the polylinker region were subcloned into the adenoviral shuttle vector pDC311 (Microbix, PD-01-25). The rat FYN shRNA targeting sequence was 5ʹ-AGGATAAAGAAGCAGCGAAACTGAC-3ʹ. The rat FGF18 shRNA targeting sequence was 5ʹ-CCTGCACTTGCCTGTGTTT-3ʹ. For Scrambled shRNA, an in-house-generated shRNA adenovirus that encodes a scrambled sequence was used as a control. Recombinant adenoviruses were generated by homologous recombination in HEK293T cells as described above. For adenovirus-mediated gene knockdown, these constructed adenovirus vectors were transfected into NRCMs at an MOI of 20× PFU/cell for 24 h. After 24 h, the knockdown efficiency was evaluated.
Chen G., An N., Shen J., Chen H., Chen Y., Sun J., Hu Z., Qiu J., Jin C., He S., Mei L., Sui Y., Li W., Chen P., Guan X., Chu M., Wang Y., Jin L., Kim K., Li X., Cong W, & Wang X. (2023). Fibroblast growth factor 18 alleviates stress-induced pathological cardiac hypertrophy in male mice. Nature Communications, 14, 1235.
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Publication 2023
Adenoviruses Adenovirus Vaccine Cells Cloning Vectors fibroblast growth factor 18, human Gene Knockdown Techniques Homologous Recombination RNA Polymerase III Short Hairpin RNA Shuttle Vectors
2
Comprehensive Immunoblotting Assay for RIG-I-Like Receptor Signaling
RNA polymerase III inhibitor (ML-60218) was from Merck Millipore. MDA5 (D74E4, rabbit mAb #5321), STING (antibody #3337), phospho-IRF-3 (Ser396) (4D4G, rabbit mAb #4947), IRF-3 (D6I4C, XP rabbit mAb #11904), RIG-I (D14G6, rabbit mAb #3743), MAVS (antibody #3993), TBK1/NAK (D1B4, rabbit mAb #3504), phospho-TBK1/NAK (Ser 172) (D52C2, rabbit mAb, #5483), IKKε (D20G4, rabbit mAb, #2905), cGAS (E9G9G, rabbit mAb, #83623), beta actin HRP conjugate (13E5, rabbit mAb # 5125), anti-rabbit IgG HRP-linked (antibody #7074), histone H2AX (antibody #2595), antibodies were from Cell Signaling Technology. GAPDH (mouse mAb, #G8795) was from Sigma Aldrich. Anti-mouse IgG HRP-linked (antibody #NA931V) was from GE Healthcare. Monoclonal anti-β-actin-peroxidase (antibody #A3854) and digitonin (D141) were from Sigma Aldrich. Anti-rabbit MEK1/2 (#8727), anti-rabbit AIF (#5318) and anti-rabbit Histone H3 (#4499) were from cell signaling, anti-rabbit ALAS1 (#MA5-35584) were from Invitrogen. TURBO DNase (2U/μl, #AM2238) was from Invitrogen. GlycoBlue Coprecipitant (AM9515) was from Applied Biosystems. TOM20 (EPR15581-39, rabbit mAb, #ab186734) and mCherry (1C51, rabbit mAb, #ab125096) were from Abcam. RIG-I (D33H10, rabbit mAb, #4200) and mouse anti-rabbit IgG (conformation specific, L27A9, mAb #3678), cell lysis buffer (#9803), protein A magnetic beads (#73778), glycine (#7005) and PMSF (#8553) were from Cell Signaling. Recombinant RNasin Ribonuclease inhibitor (#N2515) was from Promega. Secondary Alexa Fluor 488 goat anti-mouse (#A11029), Alexa Fluor 488 goat anti-rabbit (#A11034), Alexa Fluor 633 goat anti-mouse (#A21053), Alexa Fluor 647 goat anti-rabbit (#A21244), Fluoromount-G with DAPI (#00-4959-52) and SlowFade Diamond Antifade Mountant without DAPI (#S36972) were from Invitrogen. DAPI solution 1.0 mg/ml (564907) was from BD Pharmingen. Monoclonal Alexa Fluor 647 Mouse anti-H2AX (pS139) (#560447) was from BD Pharmingen. Annexin V Apoptosis Detection kit (888007) with Annexin V efluor 450 (48-8006-69) and Fixable viability Dye eFluor 780 (65-0865-14) were from eBioscience. JetPRIME (#114–07) was from Polyplus Transfection and Lipofectamine RNAiMAX (#13778075) was from Invitrogen. NucleoSpin Gel and PCR Clean-up kit (#740609) was from Macherey-Nagel. Quant-iT dsDNA Assay kit, High sensibility (#Q33120) was from Invitrogen and NEBNext DNA Library Prep kit (#E7645) and NEBNext Multiplex Oligos for Illumina (#E7600) were from New England BioLabs.
Khalfi P., Suspène R., Raymond K.A., Caval V., Caignard G., Berry N., Thiers V., Combredet C., Rufie C., Rigaud S., Ghozlane A., Volant S., Komarova A.V., Tangy F, & Vartanian J.P. (2023). Antagonism of ALAS1 by the Measles Virus V protein contributes to degradation of the mitochondrial network and promotes interferon response. PLOS Pathogens, 19(2), e1011170.
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Publication 2023
2',5'-oligoadenylate Actins alexa fluor 488 Alexa Fluor 647 Annexin A5 anti-IgG Antibodies Apoptosis beta-Actin Biological Assay Buffers Cells Chromogranin A DAPI DDX58 protein, human Deoxyribonucleases Diamond Digitonin DNA, Double-Stranded DNA Library GAPDH protein, human Glycine Goat H2AX protein, human Histone H3 IFIH1 protein, human Immunoglobulins IRF3 protein, human Lipofectamine MAVS protein, human Mice, House Mitogen-Activated Protein Kinase Kinases Peroxidase Promega Rabbits Ribonucleases RNA Polymerase III Staphylococcal Protein A Transfection
3
Generating Measles Virus Knockout Strains
The Schwarz vaccine strain MeV and MeV-ΔV were previously described [28 (link)]. Briefly, V protein expression from MeV was knock-down following a two-step PCR strategy to generate MeV-ΔV virus. These PCRs introduced a mutation interfering with RNA editing (the native sequence UUAAAAAGGGCACAGA was mutated to UUAAGAAGGGCACAGA) [28 (link)]. MeV with a GFP (MeV-GFP) was previously described [27 (link)] while MeV-ΔV-GFP was performed by cloning the GFP ORF via Sbfi and Tth111I sites from PTM3-egfp plasmid and put the fragment in the MeV-ΔV virus. Unless overwise specified, 106 THP-1 cells were seeded in 12-well plates for infection in 500μL non-supplemented RPMI. 500μL 2X supplemented RPMI was added 2 hours post-infection to restore the appropriate concentration of supplements. THP-1 cells were infected with MeV at MOI 0.1, 0.5, 1 or 3 for 24 hours. As negative control, cells were infected at a MOI 0.5 with MeV, previously inactivated at 70°C for 30 minutes. For both infections, viruses were absorbed in serum-free RPMI for a defined period at 37°C before dilution with complete RPMI. To study the role of the RNA polymerase III, inhibitor was added to the culture medium at final concentrations of 25 or 50 μM for ML-60218 (Merck Millipore) 2 hpi. Monolayers of Vero or THP-1 cells were infected with MeV, MeV-ΔV, MeV-GFP and MeV-ΔV-GFP at MOI 0.1. At various times post infection, cells were scraped into culture medium and froze at -80°C. After medium clarification of cell debris, virus titers were determined. For this purpose, Vero cells were seeded into 96-well plates (7,500 cells/well) and infected with serial 1:10 dilutions of virus sample in DMEM-5% FCS. After incubation for 7 days, cells were stained with crystal violet, and the TCID50 values were calculated by using the Karber method.
One-STrEP Tag Purification-HEK-293T (8.107) cells were either infected with rMeV/STrEP-V or with the negative control rMeV/STrEP-Cherry viruses at MOI 1. At 24 hpi, cells were lysed and tagged protein co-complexes were purified and analyzed by Western Blot as previously described [30 (link)].
Khalfi P., Suspène R., Raymond K.A., Caval V., Caignard G., Berry N., Thiers V., Combredet C., Rufie C., Rigaud S., Ghozlane A., Volant S., Komarova A.V., Tangy F, & Vartanian J.P. (2023). Antagonism of ALAS1 by the Measles Virus V protein contributes to degradation of the mitochondrial network and promotes interferon response. PLOS Pathogens, 19(2), e1011170.
Full text: Click here
Publication 2023
Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly Cells Dietary Supplements Freezing Infection Lanugo Mutation Plasmids Polymerase Chain Reaction Proteins Prunus cerasus RNA Polymerase III Serum sodium-binding benzofuran isophthalate Strains Streptococcal Infections Technique, Dilution THP-1 Cells Vaccines Vero Cells Violet, Gentian Virus Western Blotting
4
Cryo-EM Structure of C. crescentus GcrA-TAC
The C. crescentus GcrA-TACD dataset was collected on a FEI Titan Krios equipped at 300 keV with a K2 Summit direct electron detector (Gatan). The images were recorded using Serial EM software at a nominal magnification of 22 500 (1.0 Å/ pixel) in counting mode. Each image movie of 32 frames was collected by exposure of 7.6 s to give a total electron exposure of 60.8 electrons/Å2 (flux: 8 electrons/pixel/s). The 1886 images were collected with defocus range from −1.2 to −2.2 μm. Frames of individual movies were aligned using MotionCor2, and contrast-transfer-function estimations were performed using CTFFIND4. Image processing was performed with RELION 3.0. A total of 865 129 particles were auto picked and extracted from the dataset by using the 2D classified templates generated from previous C. crescentus GcrA TACU. The particles were subjected to 2D classification (N = 50, iterations 25) and the good 2D classes were subjected to 3D classification (N = 4, iterations = 25) using a 40 Å low-pass-filtered cryo-EM structure of C. crescentus GcrA TACU as the initial model. The 3D class, which contains 109 869 particles and shows clear feature of C. crescentus RNA polymerase, GcrA and promoter DNA were selected and subjected to auto refinement, CTF refinement, particle polishing, a second round of auto refinement, and postprocess, resulting in the final map. The Gold-standard Fourier-shell-correlation analysis indicated a nominal resolution of 3.79 Å at 0.143 FSC cutoff.
The structure of C. crescentus GcrA TACU was fit into the cryo-EM map. The iterative cycles of model building in Coot (Ramachandran, trans peptide, planar peptide restraints applied) and refinement in Phenix were performed.
Wu X., Yu C., Mu W., Gu Z., Feng Y, & Zhang Y. (2023). The structural mechanism for transcription activation by Caulobacter crescentus GcrA. Nucleic Acids Research, 51(4), 1960-1970.
Publication 2023
Electrons Gold Peptides Reading Frames RNA Polymerase III
5
Structural Determination of C. crescentus RNA Polymerase Complex
The C. crescentus GcrA-TACU dataset was collected on a FEI Titan Krios equipped at 300 keV with a K2 Summit direct electron detector (Gatan). The images were recorded using Serial EM software at a nominal magnification of 22 500 (1.014 Å/pixel) in counting mode. Each image movie of 32 frames was collected by exposure of 8.0 s to give a total electron exposure of 62.4 electrons/Å2 (flux: 8 electrons/pixel/s). The 3626 images were collected with defocus range from −1.2 to −2.2 μm. Frames of individual movies were aligned using MotionCor2, and contrast-transfer-function estimations were performed using CTFFIND4. Image processing was performed with RELION 3.0. A total of 481 494 particles were auto picked and extracted from the dataset by using the 2D classified templates generated from previous E. coli RNA polymerase elongation complex. The particles were subjected to 2D classification (N = 50, iterations 25) and the good 2D classes were subjected to 3D classification (N = 4, iterations = 25) using a 40 Å low-pass-filtered cryo-EM structure of E. coli RNA polymerase elongation complex as the initial model. The 3D class, which contains 309 114 particles and shows clear feature of C. crescentus RNA polymerase, GcrA and promoter DNA were selected and refined to 4.16 Å. The particles (309 114) were further subjected to focused 3D classification (N = 2, iterations = 25, without alignment) by subtracting signal outside the black mask. The 287 227 particles from the best-resolved class were reverted and subjected to auto refinement, CTF refinement, particle polishing, a second round of auto refinement, and postprocess, resulting in the final map. The Gold-standard Fourier-shell-correlation analysis indicated a nominal resolution of 3.62 Å at 0.143 FSC cutoff.
The crystal structures of GcrA-SID/σ702 (PDB: 5YIX) and GcrA-DBD/DNA (PDB: 5YIV) and cryo-EM structure of E. coli RPo (PDB:7MKL) were fit into the cryo-EM map (27 (link),33 (link)). The iterative cycles of model building in Coot (Ramachandran, trans peptide, planar peptide restraints applied) and refinement in Phenix were performed.
Wu X., Yu C., Mu W., Gu Z., Feng Y, & Zhang Y. (2023). The structural mechanism for transcription activation by Caulobacter crescentus GcrA. Nucleic Acids Research, 51(4), 1960-1970.
Publication 2023
DNA-Directed RNA Polymerase Electrons Escherichia coli Gold Peptides Reading Frames RNA Polymerase III

Top products related to «RNA Polymerase III»

1
Psuper vector by Oligoengine
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The PSUPER vector is a plasmid-based expression system used for short hairpin RNA (shRNA) production in mammalian cells. It provides a convenient platform for stable gene knockdown experiments.
2
Psilencer 2.1 u6 expression vector by Thermo Fisher Scientific
The PSilencer 2.1-U6 expression vector is a tool used for the expression of short hairpin RNA (shRNA) sequences in mammalian cells. It provides a convenient platform for the stable or transient expression of shRNA, which can be used for gene silencing experiments.
3
Plasmid mini kit by Qiagen
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The Plasmid Mini Kit is a laboratory product designed to extract and purify plasmid DNA from bacterial cultures. It provides a simple and efficient method for the isolation of high-quality plasmid DNA suitable for downstream applications.
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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Vectastain abc kit by Vector Laboratories
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The Vectastain ABC kit is a product by Vector Laboratories that is used for the detection of specific target antigens in tissue or cell samples. The kit includes reagents necessary for the avidin-biotin complex (ABC) method of immunohistochemistry. The core function of the Vectastain ABC kit is to provide a reliable and sensitive tool for the visualization of target molecules within a sample.
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Typhoon phosphorimager by Cytiva
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The Typhoon PhosphorImager is a laboratory instrument designed for high-resolution, quantitative imaging of phosphor-based signals. It utilizes a laser-based detection system to capture and digitize images of radioactively labeled samples, such as polyacrylamide gels, membranes, and plates. The Typhoon PhosphorImager provides sensitive and accurate data acquisition for a wide range of life science applications.
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Westernbright ecl kit by Advansta
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The WesternBright ECL kit is a chemiluminescent Western blotting detection system. It is designed for the sensitive detection of proteins immobilized on a membrane.
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Pcdna5 frt to vector by Thermo Fisher Scientific
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The PcDNA5/FRT/TO vector is a plasmid designed for regulated gene expression in mammalian cell lines. It contains a tetracycline-regulated promoter system, an FRT site for Flp recombinase-mediated integration, and a hygromycin resistance gene for selection.
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Ez chip chromatin immunoprecipitation kit by Merck Group
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The EZ ChIP™ Chromatin Immunoprecipitation Kit is a laboratory tool used for the extraction and analysis of DNA-protein complexes. It provides a standardized protocol for the isolation and purification of chromatin fragments bound to specific proteins of interest.
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Plb vector by Addgene
The PLB vector is a plasmid DNA construct commonly used for gene expression in bacterial systems. It contains key elements necessary for replication and selection in E. coli hosts. The vector backbone provides a platform for inserting genes of interest, enabling their expression and study in a controlled laboratory environment.

More about "RNA Polymerase III"

RNA Polymerase III (Pol III) is a crucial eukaryotic enzyme responsible for the transcription of small nuclear RNAs (snRNAs), transfer RNAs (tRNAs), and other short, untranslated RNAs.
This enzyme plays a pivotal role in gene expression and cellular processes, making it a key target for research and development.
Researchers can optimize their Pol III studies by leveraging the power of PubCompare.ai, an AI-driven platform that helps identify the most accurate and reproducible protocols from scientific literature, preprints, and patents.
By utilizing intelligent comparisons, researchers can select the best products and methodologies, ensuring their experiments deliver reliable, reproducible results and advance their research journey efficiently.
To further enhance Pol III research, scientists may employ various molecular biology tools and techniques.
The PSUPER vector and PSilencer 2.1-U6 expression vector can be utilized for RNA interference (RNAi) studies, while the Plasmid Mini Kit provides a convenient way to purify plasmid DNA.
Lipofectamine 2000 is a widely used transfection reagent for introducing nucleic acids into cells.
For downstream analysis, the Vectastain ABC kit can be used for immunohistochemistry, and the Typhoon PhosphorImager is a powerful tool for detecting and quantifying radioactive and fluorescent signals.
The WesternBright ECL kit is a popular choice for chemiluminescent Western blotting, and the PcDNA5/FRT/TO vector is commonly used for tetracycline-inducible gene expression.
Additionally, the EZ ChIP™ Chromatin Immunoprecipitation Kit can be employed to study the interactions between Pol III and its target DNA sequences, providing insights into the regulation of Pol III-dependent transcription.
The PLB vector, on the other hand, is a versatile plasmid that can be used for various applications, including reporter gene assays and protein expression.
By leveraging these tools and techniques, researchers can dive deeper into the fascinating world of RNA Polymerase III and uncover new discoveries that advance our understanding of gene expression and cellular processes.