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> Chemicals & Drugs > Amino Acid > Sincalide

Sincalide

Sincalide is a synthetic cholecystokinin analogue used to evaluate gallbladder function and stimulate gallbladder contraction.
It is commonly used in diagnostic imaging procedures and research studies related to the biliary system.
PubCompare.ai's AI-driven platform can help optimize Sincalide research protocols by easily locating relevant literature, preprints, and patents, while leveraging intelligent comparisons to identify the best protocols and prodcuts.
Streamline your Sincalide research with PubCompare.ai's intuitive solution.

Most cited protocols related to «Sincalide»

1
Cytotoxicity Assay of Anticancer Drugs
Cited 23 times
Cancer cells were first
counted, and approximately 4000 cells per well were seeded in a 96-well
cell culture plate (Corning Inc.). Then, after incubation at 37 °C
in a humidified atmosphere with 5% CO2 for 24 h, the culture
medium was replaced by a series of concentrations of drugs diluted
with the corresponding culture fluid. Five replicates were made for
each measurement, and the time of co-incubation was determined by
the efficiency of each drug. In this study, DOX (MedChemExpress Co.,
Ltd.) and CCM (Sinopharm Chemical Reagent Co., Ltd.) were co-incubated
with the cells for 24 h at 37 °C under the same conditions as
described above, whereas irinotecan hydrochloride injection (20 mg/mL,
Qilu Pharmaceutical), taxol (Aladdin), and oxaliplatin (Aladdin) were
co-incubated for 48 h. Finally, 10 μL of the CCK-8 reagent (MedChemExpress
Ltd.) was added into each well, and OD at 450 nm was measured using
a multifunction microplate reader (Infinite M200 Pro, Tecan) after
incubation for 2 h at 37 °C. The percentage each concentration
accounted for of the control was presented as cell viability. The
IC50 value was calculated using SPSS.
Cai L., Qin X., Xu Z., Song Y., Jiang H., Wu Y., Ruan H, & Chen J. (2019). Comparison of Cytotoxicity Evaluation of Anticancer Drugs between Real-Time Cell Analysis and CCK-8 Method. ACS Omega, 4(7), 12036-12042.
Publication 2019
Atmosphere Cell Survival Irinotecan Hydrochloride M-200 Malignant Neoplasms Oxaliplatin Pharmaceutical Preparations Sincalide Taxol
2
Oxidative Stress Evaluation Methods
Cited 19 times
ROS production was evaluated by DHE staining in vivo and DCFH-DA staining in vitro [38 (link), 40 (link)]. Briefly, cryosections of fresh heart samples or coverslips were stained with DHE (5 μmol/L) or DCFH-DA (5 μmol/L) in the dark at 37 °C for 30 min, and then were visualized in a blinded manner under an Olympus IX53fluorescence microscope. To further assess oxidative stress level, we measured the content of MDA, GSH, total SOD activity and NADPH oxidase activity in the myocardium or H9C2 cells according to our previous study by the commercially available kits [38 (link)]. Cell viability was determined using the CCK-8 assay kit according to the manufacturer’s protocol as described previously [9 (link), 38 (link)].
Zhang X., Hu C., Kong C.Y., Song P., Wu H.M., Xu S.C., Yuan Y.P., Deng W., Ma Z.G, & Tang Q.Z. (2019). FNDC5 alleviates oxidative stress and cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity via activating AKT. Cell Death and Differentiation, 27(2), 540-555.
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Publication 2019
Biological Assay Cells Cell Survival Cryoultramicrotomy diacetyldichlorofluorescein Heart Microscopy Myocardium NADPH Oxidase Oxidative Stress Sincalide
3
Assessing Breast Cancer Cell Sensitivity to OX
Cited 8 times
The sensitivity of BC cells to OX was measured using a CCK-8 assay kit (Dojindo Molecular Technologies, Inc.). In short, BT474/OX or MCF-7/OX cells (5 × 104 cells/well) were seeded into 96-well plates and exposed to OX in a concentration gradient from 0 to 20 μmol/L for 48 h. Then, the cells were cultured for another 2 hours after the 10 μL CCK-8 reagent (Beyotime, China) was added to the plates. The absorbance was detected using a microplate reader (BioTek China) at 450 nm.
Yao Y., Li X., Cheng L., Wu X, & Wu B. (2021). Circular RNA FAT atypical cadherin 1 (circFAT1)/microRNA-525-5p/spindle and kinetochore-associated complex subunit 1 (SKA1) axis regulates oxaliplatin resistance in breast cancer by activating the notch and Wnt signaling pathway. Bioengineered, 12(1), 4032-4043.
Publication 2021
Biological Assay Cells Hypersensitivity MCF-7 Cells Sincalide
4
Biochemical Assays for Cardiac Injury
Cited 7 times
LDH activity was measured by a commercial assay kit according to the manufacturer’s instructions. Fresh heart samples were homogenized in cold assay buffer and centrifuged at 4 °C for 15 min to remove any insoluble material. Tissue supernatants and cell medium were then incubated with the reaction mix, and the absorbance values were measured at 450 nm. Caspase3 activity in the myocardium or cultured cells was measured via detecting the fluorogenic change of Z-DEVD-AMC [26 ]. Serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) were detected by an ADVIA 2400 automatic biochemical analyzer (Siemens, Tarrytown, USA) [25 (link), 26 ]. Circulating OSTN concentrations were measured by a sandwich chemiluminescence enzyme immunoassay, as described previously [26 , 37 (link)]. Briefly, blood samples were quickly stored in microtubes containing EDTA-2Na and then centrifuged at 4 °C for 20 min with the plasma collected. Next, the plasma samples were incubated in pre-coated plates at 4 °C overnight and then probed by an anti-mouse/human OSTN rat antibody. Finally, alkaline phosphatase-conjugated donkey anti-rat antibody was applied to incubate at room temperature for an additional 1 h, and the chemiluminescence signal intensity was measured at 535 nm using a CDP-Star™ substrate with Emerald-II™ enhancer. Cell viability was assessed by the CCK-8 method, as described previously [25 (link), 51 (link)].
Zhang X., Hu C., Yuan X.P., Yuan Y.P., Song P., Kong C.Y., Teng T., Hu M., Xu S.C., Ma Z.G, & Tang Q.Z. (2021). Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity. Cell Death & Disease, 12(7), 624.
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Publication 2021
acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin Alanine Transaminase Alkaline Phosphatase Antibodies, Anti-Idiotypic Biological Assay BLOOD Buffers Caspase 3 Cells Cell Survival Chemiluminescence Chemiluminescent Assays Cold Temperature Cultured Cells Edetic Acid Enzymes Equus asinus Heart Homo sapiens Mus Myocardium Plasma Serum Sincalide Tissues Transaminase, Serum Glutamic-Oxaloacetic
5
Cell Viability Assay of Jurkat and BALL-1 Cells
Cited 7 times
Cell viability was analyzed by CCK-8 assay (Tongren Institute of Chemistry). Jurkat and BALL-1 cells (5×106) were lentivirally transduced for 72 h, and 5×104 cells/ml were seeded in 96-well plates. At 1, 2, 3, 4 and 5 days, cells were incubated with 10 µl CCK-8 solution for 2 h at 37°C with 5% CO2 and the absorbance at 450 nm was measured.
Jiang H., Tang J., Qiu L., Zhang Z., Shi S., Xue L., Kui L., Huang T., Nan W., Zhou B., Zhao C., Yu M, & Sun Q. (2021). Semaphorin 4D is a potential biomarker in pediatric leukemia and promotes leukemogenesis by activating PI3K/AKT and ERK signaling pathways. Oncology Reports, 45(4), 1.
Publication 2021
Biological Assay Cells Cell Survival Sincalide

Most recents protocols related to «Sincalide»

1
Profiling Cell Growth and Proliferation
The absorbance value of BC cells at the wavelength of 450 nm was recorded using CCK-8 at the indicated time points to plot the growth curves. In terms of the colony formation assay, BC cells (1000 cells/well) were seeded into 6-well plates, which were then routinely cultured for two weeks before their fixed staining. Cells were stained using EdU reagent and 4',6-diamidino-2-phenylindole (DAPI) to capture the cell proliferation signals under a fluorescence microscope (Leica, Wetzlar, Germany).
Wang B., Chen H., Deng Y., Chen H., Xing L., Guo Y., Wang M, & Chen J. (2023). CircDNAJC11 interacts with TAF15 to promote breast cancer progression via enhancing MAPK6 expression and activating the MAPK signaling pathway. Journal of Translational Medicine, 21, 186.
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Publication 2023
Biological Assay Cell Proliferation Cells Microscopy, Fluorescence Sincalide
2
Cell Proliferation Assay by CCK-8
Proliferation was monitored by CCK-8 assays (Meilun Biotechnology, Dalian, China). In brief, cells were inoculated into 96-well plates and cultured for 24 h at 37 °C. Then, 10 µL of CCK-8 enhanced solution was added to each well and incubated for 1.5 h at 37 °C. The absorbance at 450 nm was then determined with a microplate reader and each group was allocated three wells. All experiments were performed in triplicate.
Liao X., Ruan X., Wu X., Deng Z., Qin S, & Jiang H. (2023). Identification of Timm13 protein translocase of the mitochondrial inner membrane as a potential mediator of liver fibrosis based on bioinformatics and experimental verification. Journal of Translational Medicine, 21, 188.
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Publication 2023
Biological Assay Cells Sincalide
3
Evaluating bEnd.3 Cell Proliferation

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Publication 2023
Biological Assay Cell Proliferation Cells Culture Media Decompression Sickness Sincalide
4
Proliferation Assay of CRC Cells
The proliferation of CRC cells was assessed using a CCK-8 assay (DOJINDO, Kumamoto, Japan), in accordance to manufacturer’ instruction. The detailed concentrations of the four types of chemotherapy in our study were described in Supplementary Table 2C.
Han Y., Lu S., Song C., Xuan Y., Zhang M, & Cai H. (2023). Dual roles of TRIM3 in colorectal cancer by retaining p53 in the cytoplasm to decrease its nuclear expression. Cell Death Discovery, 9, 85.
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Publication 2023
Biological Assay Cell Proliferation Pharmacotherapy Sincalide
5
Evaluating PVT1 Knockdown Effects
Cells were grown in 96-well plates and transfected with siRNA (siPVT1-1/2). Subsequently, a Cell Counting Kit-8 (CCK-8, Dojindo, Shanghai, China) was used to detect cell proliferation at 0, 24, 48, 72, 96 and 120 h following the manufacturer’s protocol. For Transwell invasion assays, cells were inoculated into Transwell plates containing Matrigel. DMEM with 20% FBS was placed in the lower chamber. Serum-free DMEM was used to resuspend the cells, which were added to the upper chamber. After 24 h, 70% methanol was used to fix the membranes, which were then stained using 0.1% crystal violet for 10 min. After thorough washing with PBS, the membranes were imaged. The average number of invasive cells was determined by counting in three randomly chosen fields under a microscope.
Qin Z., Zhang W., Liu S., Wang Y., Peng X, & Jia L. (2023). PVT1 inhibition stimulates anti-tumor immunity, prevents metastasis, and depletes cancer stem cells in squamous cell carcinoma. Cell Death & Disease, 14(3), 187.
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Publication 2023
Biological Assay Cell Proliferation Cells matrigel Methanol Microscopy RNA, Small Interfering Serum Sincalide Tissue, Membrane Violet, Gentian

Top products related to «Sincalide»

1
Cck 8 by Dojindo Laboratories
Sourced in Japan, United States, China, Germany
CCK-8 is a cell counting kit used to measure cell viability and proliferation. It utilizes a water-soluble tetrazolium salt that is reduced by living cells, producing a colored formazan dye that can be quantified using a spectrophotometer. The amount of formazan dye produced is directly proportional to the number of living cells in the sample.
2
Cell counting kit 8 (cck8) by Dojindo Laboratories
Sourced in Japan, United States, China, Germany, United Kingdom
The Cell Counting Kit-8 is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt that produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. The amount of the formazan dye generated is directly proportional to the number of living cells.
3
Microplate reader by Bio-Rad
Sourced in United States, China, Japan, Italy, Germany, United Kingdom, Switzerland, France, Canada, Netherlands, Australia, Belgium, India
The Microplate reader is a laboratory instrument used to measure the absorbance or fluorescence of samples in a microplate format. It can be used to conduct various assays, such as enzyme-linked immunosorbent assays (ELISA), cell-based assays, and other biochemical analyses. The core function of the Microplate reader is to precisely quantify the optical properties of the samples in a multi-well microplate.
4
Cell counting kit 8 cck 8 by Dojindo Laboratories
Sourced in Japan, United States, China, Germany
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay used to measure the number of viable cells in cell proliferation and cytotoxicity assays. It utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, resulting in the formation of a colored formazan dye. The amount of formazan dye is directly proportional to the number of living cells in the culture, which can be quantified by measuring the absorbance of the solution.
5
Microplate reader by Agilent Technologies
Sourced in United States, China, Germany, Japan, United Kingdom, France, Australia, Switzerland, Ireland, Canada, India, Mongolia, Hong Kong
The Microplate reader is a versatile laboratory instrument used to measure and analyze the optical properties of samples in microplates. It is designed to quantify absorbance, fluorescence, or luminescence signals from various assays and applications.
6
Microplate reader by Thermo Fisher Scientific
Sourced in United States, China, Finland, Germany, Japan, United Kingdom, Spain, France, Italy, Australia, Sweden, Singapore, Canada, India, Denmark
The Microplate reader is a laboratory instrument designed to measure the absorbance, fluorescence, or luminescence of samples in a microplate format. It is a versatile tool used in various applications, such as enzyme-linked immunosorbent assays (ELISAs), cell-based assays, and drug discovery screens.
7
Cck 8 kit by Dojindo Laboratories
Sourced in Japan, China, United States, Finland
The CCK-8 kit is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes the highly water-soluble tetrazolium salt, WST-8, which is reduced by dehydrogenases in living cells to produce a colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.
8
Cck 8 solution by Dojindo Laboratories
Sourced in Japan, United States, China, Germany
CCK-8 solution is a colorimetric assay kit used for determining cell viability and proliferation. It contains a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, producing a colored formazan dye. The amount of formazan dye generated is directly proportional to the number of living cells in the culture, which can be quantified by measuring the absorbance.
9
Cck 8 assay by Dojindo Laboratories
Sourced in Japan, United States, China
The CCK-8 assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. It utilizes the tetrazolium salt WST-8, which is reduced by cellular dehydrogenases to produce a yellow-colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.
10
Cck 8 by Beyotime
Sourced in China, United States, Germany, Switzerland
The CCK-8 (Cell Counting Kit-8) is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, producing a colored formazan dye. The amount of formazan dye generated is directly proportional to the number of living cells, and can be measured using a spectrophotometer.

More about "Sincalide"

Sincalide, a synthetic cholecystokinin (CCK) analogue, is a versatile tool used in diagnostic imaging and research related to the biliary system.
This cholecystokinin-8 (CCK-8) derivative is known for its ability to evaluate gallbladder function and stimulate gallbladder contraction.
Researchers and clinicians can leverage Sincalide in a variety of applications, such as using it in conjunction with Cell Counting Kit-8 (CCK-8) assays to assess cell viability and proliferation.
The CCK-8 kit, which contains the CCK-8 solution, is a colorimetric assay that can be read using a microplate reader to quantify the number of living cells in culture.
PubCompare.ai's AI-driven platform can help optimize Sincalide research protocols by easily locating relevant literature, preprints, and patents, while leveraging intelligent comparisons to identify the best protocols and prodcuts.
This streamlined solution allows researchers to efficiently explore and refine their Sincalide-based studies, whether they are investigating gallbladder function, cell viability, or other applications related to the biliary system and cholecystokinin signaling.