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Stem Cell Factor

Stem Cell Factor (SCF) is a critical cytokine that regulates the proliferation, survival, and differentiation of hematopoietic stem and progenitor cells.
It plays a pivotal role in the maintenance and homeostasis of the stem cell niche.
SCF binds to the c-Kit receptor, activating signaling pathways that promote the expansion and self-renewal of stem cells.
Dysregulation of SCF/c-Kit signaling has been implicated in the pathogenesis of various hematological malignancies and other diseases.
Understanding the mechanisms of SCF-mediated stem cell biology is crucial for advancing regenerative medicine and developing targeted therapies.
PubCompare.ai offers a powerful AI-driven platform to optimize SCF research by effortlesly locating the most accurate and rproducible protocols from scientific literature, pre-prints, and patents.

Most cited protocols related to «Stem Cell Factor»

Ribosome profiling and mRNA sequencing of miRNA and knockout effects

Cited 94 times

HeLa cells were transfected with 100 nM miRNA duplex as described17 (link) and harvested 12 and 32 h later. Haematopoietic progenitors were isolated from wild-type (WT) and mir-223 knockout (KO) male mice and cultured in media containing granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) as described16 (link) for six days before harvesting. Just before harvesting, translation was arrested using cycloheximide for 8 min at 37 °C. Harvested cells were partitioned into two portions for ribosome profiling and mRNA profiling. Ribosome profiling was performed as outlined in Fig. 1a. For mRNA profiling, poly(A)+ mRNA was randomly fragmented by partial alkaline hydrolysis and size-selected RNA fragments were used to construct libraries for high-throughput sequencing. Illumina sequencing reads were mapped using the Bowtie short-read mapping program32 (link). An iterative mapping strategy was adopted to obtain unique genome-matching and splice junction-spanning reads. A set of non-redundant transcripts served as our reference transcript database, which was used to map splice junction-spanning reads, quantify gene expression, and quantify RPF and mRNA-Seq changes.

Guo H., Ingolia N.T., Weissman J.S, & Bartel D.P. (2010). Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature, 466(7308), 835-840.

Publication 2010

Cells Culture Media Cycloheximide Gene Expression Genome Granulocyte Colony-Stimulating Factor HeLa Cells Hematopoietic System Hydrolysis Males Mice, Knockout MicroRNAs mRNA, Polyadenylated RNA, Messenger Stem Cell Factor

Differentiation of Murine Bone Marrow Cells

Cited 92 times

Bone marrow cells were collected from the femurs and tibiae of wild-type BALB/c mice (Taconic) by flushing the opened bones with Iscove's Modified Dulbecco's Medium (IMDM; Invitrogen). Red blood cells were lysed in dH₂O followed by the addition of 10X PBS. After centrifugation, the cells were washed once in PBS containing 0.1% BSA. The bone marrow cells were cultured at 10⁶/mL in media containing RPMI 1640 (Invitrogen) with 20% FBS (Cambrex), 100 IU/mL penicillin and 10 μg/mL streptomycin (Cellgro), 2 mM glutamine (Invitrogen), 25 mM HEPES and 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco) and 50 μM β-mercaptoethanol (Sigma) and supplemented with 100 ng/mL stem-cell factor (SCF; PeproTech) and 100 ng/mL FLT3-Ligand (FLT3-L; PeproTech) from day 0 to day 4. On day 4, the media containing SCF and FLT3-L was replaced with media containing 10 ng/mL recombinant mouse interleukin-5 (rmIL-5; R&D Systems) only. On day 8, the cells were moved to new flasks and maintained in fresh media supplemented with rmIL-5. Every other day, from this point forward, one-half of the media was replaced with fresh media containing rmIL-5, and the concentration of the cells was adjusted each time to10⁶ /mL. Cells were enumerated at day 0 and on days indicated thereafter in a hemocytometer, and 50,000 cells were subjected to cytospin (Thermo Shandon, Pittsburgh, PA). The cytospin preparations were fixed and stained using a modified Giemsa preparation (Diff Quik, Dade Behring AG, Dudingen, Switzerland).

Dyer K.D., Moser J.M., Czapiga M., Siegel S.J., Percopo C.M, & Rosenberg H.F. (2008). Functionally competent eosinophils differentiated ex vivo in high purity from normal mouse bone marrow. Journal of immunology (Baltimore, Md. : 1950), 181(6), 4004-4009.

Publication 2008

2-Mercaptoethanol 3,3'-diallyldiethylstilbestrol Amino Acids, Essential Bone Marrow Cells Bones Cells Centrifugation Diff Quik Erythrocytes Femur flt3 ligand FLT3 protein, human Glutamine HEPES Interleukin-5 Mice, Inbred BALB C Mus Penicillins Pyruvate Sodium Stain, Giemsa Stem Cell Factor Streptomycin Tibia

Murine Hematopoietic Stem Cell Transplantation

Cited 20 times

All animal studies were approved by the Animal Use and Care Committees of the Center for Blood Research and Harvard Medical School. Balb/c mice (The Jackson Laboratory or Taconic Farms) from 6 to 12 wk of age were used in all experiments. In some experiments, male donor mice were primed by intravenous injection with 5-fluorouracil (5-FU; 200 mg/kg) 4 d before harvest. Male donors were killed by CO₂ asphyxiation, femur and tibia were collected, and bone marrow was harvested by flushing with syringe and 26-gauge needle. Cells were counted and plated without removal of erythrocytes at 2 × 10⁷ cells per 10-cm plate in prestimulation medium (38 (link)) of DME, 15% (vol/vol) inactivated FCS, 5% (vol/vol) WEHI-3B conditioned medium, penicillin/ streptomycin, 1.0 μg/ml ciprofloxacin, 200 μM l-glutamine, 6 ng/ml recombinant murine IL-3 (Genzyme), 10 ng/ml recombinant murine IL-6 (Genzyme), and 50–100 ng/ml recombinant murine stem cell factor (SCF; PeproTech). With non–5-FU–treated marrow, 10 ng/ml recombinant murine IL-7 (Genzyme) was also included. After prestimulation for 24 h at 37°C, viable cells were counted and transduced with retroviral stocks in the same medium containing 50% retroviral supernatant, 10 mM Hepes, pH 7.4, and 2 μg/ml polybrene. To increase transduction efficiency (39 (link)), virus and cells were cosedimented at 1,000 g for 90 min in a Sorvall RT-6000 centrifuge. Medium was changed after a 2–4-h adsorption period. At 48 h, a second round of transduction and cosedimentation was performed, and the cells were collected 2 h later, washed once in HBSS, and counted. Recipient female mice were prepared by two doses of 450-cGy gamma irradiation separated by 3 h. Transduced marrow cells were transplanted by injection of 0.2–0.5 × 10⁶ cells (5-FU–treated marrow) or 1.0 × 10⁶ cells (non–5-FU–treated marrow) in 0.4–0.5 ml HBSS into the lateral tail vein. After transplant, recipients were housed in microisolator cages supplied with acidified (pH 2.0) water.

Li S., Ilaria RL J.r., Million R.P., Daley G.Q, & Van Etten R.A. (1999). The P190, P210, and P230 Forms of the BCR/ABL Oncogene Induce a Similar Chronic Myeloid Leukemia–like Syndrome in Mice but Have Different Lymphoid Leukemogenic Activity. The Journal of Experimental Medicine, 189(9), 1399-1412.

Publication 1999

Adsorption Animals Asphyxia BLOOD Bone Marrow Cells Ciprofloxacin Culture Media, Conditioned Donors Erythrocytes Females Femur Gamma Rays Glutamine Grafts Hemoglobin, Sickle HEPES Males Marrow Mice, Inbred BALB C Mus Needles Penicillins Polybrene Radiotherapy Retroviridae Stem Cell Factor Streptomycin Syringes Tail Tibia Tissue Donors Veins Virus

Cardiac Differentiation and Atrial Specification of hESCs

Cited 18 times

A transgenic NKX2-5-eGFP/w hESC line that faithfully reports endogenous NKX2.5 expression by GFP was described previously (Elliott et al, 2011 (link)). Undifferentiated hESCs were maintained on irradiated mouse embryonic fibroblasts, and cardiac differentiation was induced using a spin EB protocol. Briefly, hESCs were harvested and resuspended on day 0 in BPEL medium (Ng et al, 2008 (link)) containing 20–30 ng/ml hActivin-A (R&D Systems), 20–30 ng/ml bone morphogenetic protein 4 (R&D Systems), 40 ng/ml stem cell factor (Stem Cell Technologies), 30 ng/ml vascular endothelial growth factor (R&D Systems) and 1.5 μmol/l CHIR 99021 (Axon Medchem). EBs were refreshed on day 3 with BPEL and then transferred to gelatin-coated dishes on day 7.
To induce atrial specification in hESCs, cardiac differentiation was initiated as described above and 1 μmol/l all-trans retinoic acid (RA) (Sigma) was added on day 4 of differentiation. Cells were refreshed with BPEL on day 7 of differentiation.

Devalla H.D., Schwach V., Ford J.W., Milnes J.T., El-Haou S., Jackson C., Gkatzis K., Elliott D.A., Chuva de Sousa Lopes S.M., Mummery C.L., Verkerk A.O, & Passier R. (2015). Atrial-like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial-selective pharmacology. EMBO Molecular Medicine, 7(4), 394-410.

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Publication 2015

Animals, Transgenic Axon Bone Morphogenetic Protein 4 Cells Chir 99021 Embryo Fibroblasts Gelatins Heart Heart Atrium Human Embryonic Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal Mus Stem Cell Factor Stem Cells Tretinoin Vascular Endothelial Growth Factors

Expansion of Peripheral Blood Mononuclear Cells

Cited 10 times

QQ culture medium of Stem Line II (Sigma‐Aldrich, St. Louis, MO) contained the 5 human recombinant proteins: stem cell factor (SCF); thrombopoietin (TPO); Flt‐3 ligand; vascular endothelial growth factor (VEGF); and interleukin (IL)‐6. Then, isolated PBMNCs were cultured for 7 days at the cell density of 2×10⁶ cells/2 mL QQ culture medium per well of 6‐well Primaria tissue culture plate (BD Falcon; BD Biosiences, San Jose, CA). Cell density in QQ culture was corresponded to the approximate density of 1×10⁶ MNCs in 1 mL of PB. Culture well plates and the contents of QQ culture medium are listed in Tables 1 and 2.

Masuda H., Tanaka R., Fujimura S., Ishikawa M., Akimaru H., Shizuno T., Sato A., Okada Y., Iida Y., Itoh J., Itoh Y., Kamiguchi H., Kawamoto A, & Asahara T. (2014). Vasculogenic Conditioning of Peripheral Blood Mononuclear Cells Promotes Endothelial Progenitor Cell Expansion and Phenotype Transition of Anti‐Inflammatory Macrophage and T Lymphocyte to Cells With Regenerative Potential. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(3), e000743.

Publication 2014

Cell Culture Techniques Cells Culture Media flt3 ligand Interleukin-6 NR4A2 protein, human Stem, Plant Stem Cell Factor Thrombopoietin Tissues Vascular Endothelial Growth Factors

Most recents protocols related to «Stem Cell Factor»

Hematopoietic Progenitor Cell Assays

For HPC colony assays, BM cells flushed from femurs of the indicated mice were plated at 5×10⁴ cells/mL in 1% methylcellulose culture medium with 0.1 mM hemin (MilliporeSigma), 30% FBS, 1 U/mL recombinant human erythropoietin (rhEPO) (Amgen), 50 ng/mL recombinant mouse stem cell factor (rmSCF) (R&D Systems; catalog 455-MC), and 5% vol/vol pokeweed mitogen mouse splenic cell conditioned medium. Colonies were scored after 6 days of incubation in 5% CO₂ and lowered 5% O₂ in a humidified chamber, and granulocyte-macrophage colony-forming units (CFU-GM), erythrocyte burst-forming units (BFU-E), and granulocyte, erythrocyte, macrophage, and megakaryocyte colony-forming units (CFU-GEMM) were distinguished by morphology of colonies. The total numbers of colonies per femur were calculated. For high specific activity tritiated thymidine kill assays, BM cells were treated with 50 μCi high specific activity [3H]Tdr (20 Ci/mmol; DuPont NEN) at RT for 40 minutes and then washed twice prior to plating for HPC colony assays (37 (link), 38 (link)).

Tikka C., Beasley L., Xu C., Yang J., Cooper S., Lechner J., Gutch S., Kaplan M.H., Capitano M, & Yang K. (2023). BATF sustains homeostasis and functionality of bone marrow Treg cells to preserve homeostatic regulation of hematopoiesis and development of B cells. Frontiers in Immunology, 14, 1026368.

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Publication 2023

Biological Assay Cells Colony-Forming Units, Granulocyte-Erythroid-Macrophage-Megakaryocyte Culture Media Culture Media, Conditioned Erythrocytes Erythropoietin Femur Granulocyte Granulocyte-Macrophage Colony Forming Units Hemin Homo sapiens Macrophage Megakaryocytes Methylcellulose Mus Pokeweed Mitogens Spleen Stem Cell Factor

CD34+ Cell Expansion with hBMSCs

Human CML K562 cells and Human bonemesenchymal stem cells (hBMSCs) were acquired from the American Type Culture Collection (ATCC). hBMSCs were maintained in DMEM/F12 including 10% fetal bovine serum. K562 CD34⁺ cells were isolated by using anti-CD34 magnetic beads. CD34⁺ cells were incubated with or without hBMSCs at 37 °C with 5% CO₂ in high GF-supplemented(stem cell factor 100 pg/mL, SCF + FL + TPO, 50 ng/ml) serum-free expansion medium(Stem Cell Technologies, Vancouver, BC, Canada). Baicalein and IM were purchased from Melonepharma (Dalian, China). Anti-human GM-CSF (α-rhGM-CSF) was acquired from PeproTech Inc.(USA).

Xu X., Ji S., Chen Y., Xia S., Li Y., Chen L., Li Y., Zhang F., Zhang Z, & Zheng S. (2023). Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34+ cells. Cell Communication and Signaling : CCS, 21, 47.

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Publication 2023

baicalein Cells Fetal Bovine Serum Granulocyte-Macrophage Colony-Stimulating Factor Homo sapiens K562 Cells regramostim Serum Stem Cell Factor Stem Cells

Eosinophil Isolation and Reconstitution for AAA

Mouse EOS were prepared and cultured from WT (C57BL/6) and CD45.1 transgenic mice (002014, C57BL/6, N25) as previously reported.^[¹¹, ⁴⁴^] In brief, bone‐marrow cells were collected from mouse femurs and tibias, centrifuged for 5 min at 300 g, followed by lysing red blood cells using the ACK Lysing buffer (A1049201, Thermo Fisher Scientific). Cells were the suspended in 10 mL of PBS prior to cell counting. Cells with a concentration of 10⁶ cells mL⁻¹ were plated in an 80 cm² flask and cultured in a base media containing mouse stem cell factor (100 ng mL⁻¹, 250‐03, PeproTech) and mouse Flt‐3‐ligand (100 ng mL⁻¹, 250‐31L, PeproTech) for 2 d. On day 2, one‐half of the media from each flask was replaced with a fresh medium containing mouse stem cell factor and Flt‐3‐ligand. On day 4, culture media were replaced with the same media containing recombinant mouse IL5 (10 ng mL⁻¹, 405‐ML, R&D Systems). Half of the culture media were changed with fresh media containing IL5 every 2 d until the 14th day. On day 14, cells were collected, and cell purity was tested by FACS analysis with CD11b (1:200, 17‐0112‐83, eBioscience) and Siglec‐F (1:200, 12‐1702‐82, eBioscience). For reconstitution, EOS were collected and counted on day 14. Each recipient mouse received one dose of 1 × 10⁷ EOS by intravenous (i.v.) injection before AAA operation. To prepare the EOS lysate for cell treatment, 1 × 10⁷ EOS were resuspended in 1 mL culture medium. After 5 cycles of freezing and thawing, cells were centrifuged at 3000 rpm. Supernatants were then collected and stored at −80 °C.

Zhang Y., Liu T., Deng Z., Fang W., Zhang X., Zhang S., Wang M., Luo S., Meng Z., Liu J., Sukhova G.K., Li D., McKenzie A.N., Libby P., Shi G, & Guo J. (2023). Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils. Advanced Science, 10(7), 2206958.

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Publication 2023

Bone Marrow Cells Buffers Cells Culture Media Erythrocytes Femur flt3 ligand ITGAM protein, human Mice, Transgenic Mus Sialic Acid Binding Ig-like Lectin 1 Stem Cell Factor Tibia

Primary AML and MDS Cell Culture Protocol

Primary AML and MDS cells were collected from patients who had consented to research protocols approved by the Institutional Review Board at The University of Texas MD Anderson Cancer Center for analysis of hematologic malignancies. BM aspirate mononuclear cells from patients with MDS were purified by Ficoll density centrifugation using standard procedures. Lymphocytes were isolated by using Lymphocyte Separation Medium (SIGMA).
Primary AML peripheral blood mononuclear cells (PBMCs) were cultured in serum-free Expansion Medium (Cat. 09650) supplemented with BIT 9500 Serum Substitute (Cat. 09500; both from STEMCELL Technologies Inc., Vancouver, BC, Canada) and cytokines including stem cell factor (SCF, 100 ng/mL, Cat. 300-07), Flt3 ligand (50 ng/mL, Cat. 300 – 19), IL3 (20 ng/mL, Cat. 200-03), and G-CSF (20 ng/mL, Cat. 300 – 23; all from Peprotech, Rocky Hill, NJ) and StemRegenin 1 (1 μM, Cat. S2858; Selleck Chemicals LLC, Houston, TX).

Konopleva M., DiNardo C., Bhagat T., Baran N., Lodi A., Saxena K., Cai T., Su X., Skwarska A., Guerra V., Kuruvilla V., Konoplev S., Gordon-Mitchell S., Pradhan K., Aluri S., Collins M., Sweeney S., Busquet J., Rathore A., Deng Q., Green M., Grant S., Demo S., Choudhary G., Sahu S., Agarwal B., Spodek M., Thiruthuvanathan V., Will B., Steidl U., Tippett G., Burger J., Borthakur G., Jabbour E., Pemmaraju N., Kadia T., Komblau S., Daver N., Naqvi K., Short N., Garcia-Manero G., Tiziani S, & Verma A. (2023). Glutaminase inhibition in combination with azacytidine in myelodysplastic syndromes: Clinical efficacy and correlative analyses. Research Square.

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Publication Preprint 2023

Cells Centrifugation Culture Media Cytokine Ethics Committees, Research Ficoll flt3 ligand Granulocyte Colony-Stimulating Factor Hematologic Neoplasms Lymphocyte Malignant Neoplasms Patients PBMC Peripheral Blood Mononuclear Cells Serum Stem Cell Factor Stem Cells StemRegenin 1

Manufacturing of Autologous Gene-Modified Hematopoietic Stem Cells

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Publication 2023

Apheresis Body Weight Bone Marrow Cell Culture Techniques Cells Cloning Vectors Cytokine Dinoprostone flt3 ligand General Anesthesia Granulocyte Colony-Stimulating Factor Hematopoietic System Homo sapiens Interleukin-3 interleukin 20 Patients Pharmaceutical Adjuvants plerixafor Serum Stem Cell Factor Therapies, Biological Therapy, Gene Thrombopoietin Treatment Protocols

Top products related to «Stem Cell Factor»

Stem cell factor (scf) by Thermo Fisher Scientific

Sourced in United States, Canada, United Kingdom, Germany, Japan, Switzerland, Israel, Macao

The SCF is a versatile laboratory instrument designed to perform supercritical fluid extraction and chromatography. It utilizes the unique properties of supercritical fluids, such as adjustable solvent power and low viscosity, to efficiently extract, fractionate, and purify a wide range of compounds. The core function of the SCF is to provide researchers and analysts with a powerful tool for sample preparation, purification, and analysis across various industries and applications.

Interleukin 3 (il 3) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Canada, Israel, Switzerland, France, Macao, Japan

IL-3 is a recombinant protein that supports the growth and differentiation of hematopoietic stem and progenitor cells. It plays a critical role in the regulation of blood cell production.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Stem cell factor (scf) by R&D Systems

Sourced in United States, Canada, United Kingdom, Japan

The SCF is a laboratory instrument designed for the separation and purification of complex biological samples. The core function of the SCF is to utilize supercritical fluid technology to extract, fractionate, and concentrate target analytes from various matrices.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Interleukin 6 (il 6) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, China, Austria, Canada, Japan, Israel, France, Sweden, Italy, Switzerland

IL-6 is a lab equipment product that measures the concentration of interleukin-6 (IL-6), a cytokine involved in various biological processes. The core function of this product is to quantify IL-6 levels in samples.

Interleukin 3 (il 3) by R&D Systems

Sourced in United States, United Kingdom, Canada, Germany, Israel

IL-3 is a recombinant human cytokine that supports the growth and differentiation of hematopoietic progenitor cells. It is a critical factor for the proliferation and maturation of myeloid and erythroid cells.

Thrombopoietin by Thermo Fisher Scientific

Sourced in United States, Canada, United Kingdom

Thrombopoietin is a glycoprotein that regulates the production and differentiation of platelets in the bone marrow. It is a key regulator of megakaryocyte and platelet production.

L glutamine by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon

L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

Iscove modified dulbecco media (imdm) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, China, Germany, Canada, France, Italy, Austria, Israel, Switzerland, Belgium, Australia

IMDM (Iscove's Modified Dulbecco's Medium) is a cell culture medium formulated for the growth and maintenance of a wide variety of cell types, including hematopoietic cells. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.

What are the different types or variations of Stem Cell Factor (SCF)?

Stem Cell Factor (SCF) exists in both a soluble and a membrane-bound form. The soluble form is produced by proteolytic cleavage of the membrane-bound form, which allows it to be released and exert its effects on target cells. Additionally, there are different isoforms of SCF that result from alternative splicing of the SCF gene, leading to variations in the structure and biological activities of the protein.

What are some common challenges or considerations when working with Stem Cell Factor (SCF)?

One key challenge when using SCF is ensuring the stability and bioactivity of the protein, as it can be sensitive to degradation and lose potency over time. Proper storage conditions, such as maintaining appropriate temperature and avoiding repeated freeze-thaw cycles, are important to preserve SCF activity. Additionally, the concentration and purity of SCF preparations can vary, which can impact the consistency and reproducibility of experimental results.

How can Stem Cell Factor (SCF) be used in different applications?

SCF has a wide range of applications in both research and clinical settings. In research, SCF is commonly used to support the expansion and self-renewal of hematopoietic stem and progenitor cells in cell culture systems. It is also used to investigate the role of SCF/c-Kit signaling in the regulation of stem cell fate and differentiation. In the clinical setting, SCF has been explored for its potential to stimulate hematopoietic stem cell mobilization and engraftment in the context of stem cell transplantation and regenerative medicine.

How can PubCompare.ai help with optimizing Stem Cell Factor (SCF) research?

PubCompare.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform's AI-driven analysis can help researchers identify the most effective protocols related to Stem Cell Factor for their specific research goals. By highlighting key differences in protocol effectiveness, PubCompare.ai enables you to choose the best option for reproducibility and accuracy, accelerating your Stem Cell Factor research.

Is there anything else I should know about Stem Cell Factor (SCF) and its use in research?

One important consideration when working with SCF is the potential for batch-to-bacth variability, which can impact the consistency of your results. PubCompare.ai's AI-driven platform can help you identify the most reliable and reproducible SCF protocols, reducing the risk of such inconsistencies. Additionally, understanding the complex interplay between SCF and other signaling pathways, such as the Notch and Wnt pathways, is crucial for optimizing stem cell culture and differentiation protocols.

More about "Stem Cell Factor"

Stem Cell Factor (SCF), also known as Kit ligand (KL) or Steel factor, is a critical cytokine that regulates the proliferation, survival, and differentiation of hematopoietic stem and progenitor cells.
It plays a pivotal role in the maintenance and homeostasis of the stem cell niche.
SCF binds to the c-Kit (CD117) receptor, activating signaling pathways that promote the expansion and self-renewal of stem cells.
Dysregulation of SCF/c-Kit signaling has been implicated in the pathogenesis of various hematological malignancies, such as acute myeloid leukemia (AML) and myeloproliferative neoplasms (MPNs), as well as other diseases.
Understanding the mechanisms of SCF-mediated stem cell biology is crucial for advancing regenerative medicine and developing targeted therapies.
Researchers studying SCF often utilize related factors like Interleukin-3 (IL-3), Penicillin/Streptomycin, Fetal Bovine Serum (FBS), Interleukin-6 (IL-6), Thrombopoietin, and L-Glutamine to culture and maintain stem and progenitor cells.
The Iscove's Modified Dulbecco's Medium (IMDM) is a commonly used cell culture medium that supports the growth and differentiation of hematopoietic cells.
PubCompare.ai offers a powerful AI-driven platform to optimize SCF research by effortlesly locating the most accurate and reproducible protocols from scientific literature, pre-prints, and patents.
With its cutting-edge technology, researchers can quickly identify the best protocols and products to accelerate their SCF-related studies.