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Sterol Regulatory Element Binding Protein 1c

Sterol Regulatory Element Binding Protein 1c (SREBP-1c) is a transcription factor that plays a key role in the regulation of lipid and cholesterol metabolism.
It is a member of the sterol regulatory element-binding protein (SREBP) family, which are known to activate genes involved in the synthesis of fatty acids, triglycerides, and cholesterol.
SREBP-1c is the predominant isoform expressed in liver and adipose tissue, and its activity is modulated by various nutritional and hormonal signals.
Dysregulation of SREBP-1c has been implicated in the development of metabolic disorders such as non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes.
Understanding the regulatory mechanisms and functions of SREBP-1c is crucial for developing targeted therapies for these conditions.

Most cited protocols related to «Sterol Regulatory Element Binding Protein 1c»

1
Analyzing Hepatic Lipid Metabolism Genes
Cited 5 times
The total RNA was isolated from frozen tissue samples and cultured hepatocytes. RNA isolation and real-time RT-PCR were conducted as previously described [35] . The mRNA levels were analyzed for SREBP1c, carbohydrate responsive element-binding protein (ChREBP), ACC1, FAS, CPT1a, VLDLr, glucokinase, glucose-6-phosphatase, TNFα, and/or IL-6.
Guo X., Li H., Xu H., Halim V., Zhang W., Wang H., Ong K.T., Woo S.L., Walzem R.L., Mashek D.G., Dong H., Lu F., Wei L., Huo Y, & Wu C. (2012). Palmitoleate Induces Hepatic Steatosis but Suppresses Liver Inflammatory Response in Mice. PLoS ONE, 7(6), e39286.
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Publication 2012
ACACA protein, human carbohydrate-binding protein carnitine palmitoyltransferase 1A, human Freezing Glucokinase Glucose 6-Phosphatase Hepatocyte isolation Real-Time Polymerase Chain Reaction RNA, Messenger Sterol Regulatory Element Binding Protein 1c Tissues Tumor Necrosis Factor-alpha VLDLR protein, human
2
Western Blot Analysis of Insulin Signaling
Cited 5 times
Western analyses were conducted as described (2 (link),4 (link)), using: rabbit polyclonal anti-PKC-ζ/λ antiserum (Santa Cruz Biotechnologies); rabbit polyclonal anti-PKB antiserum (Upstate Cell Signalling); rabbit polyclonal anti-phospho-serine-473-PKB antiserum (Cell Signalling Technology): rabbit polyclonal anti-IRS-1 and anti-IRS-2 antisera (Upstate Cell Signalling); rabbit polyclonal anti-IRS-2 antiserum ((Upstate Cell Signalling); rabbit polyclonal anti-p85/PI3K antiserum ((Upstate Cell Signalling); rabbit polyclonal anti-SREBP-1c antiserum (Santa Cruz Biotechnologies); and rabbit polyclonal anti-phospho-serine-307-IRS-1 (Upstate Cell Signalling).
Sajan M.P., Standaert M.L., Rivas J., Miura A., Kanoh Y., Soto J., Taniguchi C.M., Kahn C.R, & Farese R.V. (2009). Role of atypical protein kinase C in activation of sterol regulatory element binding protein-1c and NFκB in rodent diabetic liver and relationships to hyperlipidemia and insulin resistance. Diabetologia, 52(6), 1197-1207.
Publication 2009
Immune Sera IRS1 protein, human IRS2 protein, human PIK3CB protein, human Rabbits Serine Sterol Regulatory Element Binding Protein 1c
3
Protein Expression Analysis in Mouse Adipose Tissue
Cited 4 times
Protein expression was evaluated by western blot. Frozen mouse epididymal fat tissue was homogenized in liquid nitrogen. Tissue was lysed in RIPA lysis buffer (Sigma) containing 1% PI. Cell lysate was kept on ice for 1 h and subsequently centrifuged at 13,000 rpm for 20 min at 4°C. Total proteins (30 μg protein/test) were isolated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane (DC, Invitrogen). The membrane was blocked with 5% skim milk and subsequently incubated at 4°C with the following primary antibodies (1:5000 dilution): anti-AMPK, anti-SREBP-1c, anti-FAS, anti-ACC, anti-PPARγ, anti-C/EBPα, anti-FABP4, and anti-adiponectin (Cell Signaling Technology, Danvers, MA, USA). After washing, the membrane was incubated with IgG HRP-conjugated secondary antibody (1:2000) for 2 h at room temperature. Ponceau S was used for staining the protein bands. β-actin was used as the loading control. Proteins were visualized using enhanced-chemiluminescence (ECL) location reagent and quantified with the ImageJ program.
Fan M., Choi Y.J., Tang Y., Bae S.M., Yang H.P, & Kim E.K. (2019). Efficacy and Mechanism of Polymerized Anthocyanin from Grape-Skin Extract on High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease. Nutrients, 11(11), 2586.
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Publication 2019
Actins ADIPOQ protein, human Antibodies Buffers Cells Chemiluminescence Epididymis FABP4 protein, human Freezing IGG-horseradish peroxidase Immunoglobulin G Immunoglobulins Milk, Cow's Mus Nitrocellulose Nitrogen ponceau S PPAR gamma Proteins Radioimmunoprecipitation Assay SDS-PAGE Sterol Regulatory Element Binding Protein 1c Technique, Dilution Tissue, Adipose Tissue, Membrane Tissues Western Blotting
4
Quantitative Real-Time PCR Analysis
Cited 4 times
Tissue samples were homogenized in TRIzol (Invitrogen), RNA was extracted, reverse transcribed to cDNA (Verso cDNA Synthesis Kit; Thermo Scientific) and subjected to qPCR analysis (Light Cycler 480 II; Roche) – all according to manufacturer’s instruction as previously described63 (link). The following primers pairs were used: Nr3c1 For CCATAATGGCATACCGAAGC Rev AGGCCGCTCAGTGTTTTCTA – Adbr2 For TAGCGATCCACTGCAATCAC Rev ATTTTGGCAACTTCTGGTGC – Ppargc1a For CTGCTAGCAAGTTTGCCTCA Rev AGTGGTGCAGTGACCAATCA – Ucp1 For TCAGCTGTTCAAAGCACACA Rev GTACCAAGCTGTGCGATGTC – Ucp2 For TCCTGCTACCTCCCAGAAGA Rev CTGAGACCTCAAAGCAGCCT – Srebp1c For CTGTCTCACCCCCAGCATAG Rev GATGTGCGAACTGGACACAG – Ppara For CATGGGGAGAGAGGACAGA Rev AGTTCGGGAACAAGACGTTG – Col1a1 For GGTTTCCACGTCTCACCATT Rev ACATGTTCAGCTTTGTGGACC – Col1a2 For AGCAGGTCCTTGGAAACCTT Rev AAGGAGTTTCATCTGGCCCT – Ccl2 For AGATGCAGTTAACGCCCCAC Rev TGTCTGGACCCATTCCTTCTTG – Ccl3 For ACCATGACACTCTGCAACCAAG Rev TTGGAGTCAGCGCAGATCTG – Cxcl1 For ACCCAAACCGAAGTCATAGC Rev TCTCCGTTACTTGGGGACAC; Il23a For AGGCTCCCCTTTGAAGATGT Rev TTGTGACCCACAAGGACTCA – Saa1 For CATTTGTTCACGAGGCTTTCC Rev GTTTTTCCAGTTAGCTTCCTTCATGT – Saa2 For TGTGTATCCCACAAGGTTTCAGA Rev TTATTACCCTCTCCTCCTCAAGCA –Beta-actin (Actb) For GGCCCAGAGCAAGAGAGGTA Rev GGTTGGCCTTAGGTTTCAGG. mRNA expression of each gene was compared to Beta-actin expression (endogenous house-keeping gene control).
Giles D.A., Moreno-Fernandez M.E., Stankiewicz T.E., Graspeuntner S., Cappelletti M., Wu D., Mukherjee R., Chan C.C., Lawson M.J., Klarquist J., Sünderhauf A., Softic S., Kahn C.R., Stemmer K., Iwakura Y., Aronow B.J., Karns R., Steinbrecher K.A., Karp C.L., Sheridan R., Shanmukhappa S.K., Reynaud D., Haslam D.B., Sina C., Rupp J., Hogan S.P, & Divanovic S. (2017). Thermoneutral housing exacerbates non-alcoholic fatty liver disease in mice and allows for sex-independent disease modeling. Nature medicine, 23(7), 829-838.
Publication 2017
Anabolism beta-Actin CCL2 protein, human CCL3 protein, human COL1A2 protein, human CXCL1 protein, human DNA, Complementary Gene Expression Genes, Housekeeping IL23A protein, human Light Oligonucleotide Primers PPARGC1A protein, human RNA, Messenger SAA2 protein, human Sterol Regulatory Element Binding Protein 1c Tissues trizol UCP1 protein, human
5
Generating Srebp-1c Overexpressing Cell Lines
Cited 4 times
The Srebp‐1c revertant was generated using retroviral infection. The vectors, termed pMXs‐AMNN‐puro and pMXs‐AMNN‐puro‐Srebp‐1c mature form, were transfected into Plat‐E cells (kindly provided by T. Kitamura, University of Tokyo, Japan) with FuGENE®6 (Promega, Madison, WI, USA), according to the manufacturer's protocol. Each virus‐containing culture supernatant was collected 2 d after transfection and filtered through 0.22‐μm filters (Millipore, Billerica, MA, USA). To obtain stable cell lines, Srebp‐1c KO‐MEFs were incubated with virus‐containing medium for 2 days, followed by selection with 5 μg mL−1 puromycin for 5 days.
Fujii N., Narita T., Okita N., Kobayashi M., Furuta Y., Chujo Y., Sakai M., Yamada A., Takeda K., Konishi T., Sudo Y., Shimokawa I, & Higami Y. (2017). Sterol regulatory element‐binding protein‐1c orchestrates metabolic remodeling of white adipose tissue by caloric restriction. Aging Cell, 16(3), 508-517.
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Publication 2017
Cell Lines Cells Cloning Vectors FuGene PLAT protein, human Promega Puromycin Retroviridae Infections Sterol Regulatory Element Binding Protein 1c Transfection Virus

Most recents protocols related to «Sterol Regulatory Element Binding Protein 1c»

1
Western Blot Analysis of Metabolic Regulators
Total lysates containing 30 μg protein for liver, skeletal muscle and vWAT were loaded in each lane and were electrophoresed on SDS-PAGE gels and transferred to nitrocellulose membrane and membranes were blocked with 5% (w/v) nonfat dry milk (in TBS-T). Primary antibodies were diluted in TBS with 0.01% (v/v) Tween 20 (TBS-T) and 5% (w/v) bovine serum albumin (BSA), while secondary antibodies were diluted in TBS with 0.01% (v/v) Tween 20 (TBS-T) and 5% (w/v) nonfat dry milk. Membranes were probed with the following antibodies: polyclonal anti PTP1B (Abcam- 1:750 dilution), monoclonal anti-Total OXPHOS (Abcam- 1:500 dilution), polyclonal anti SIRT-1 (Abcam- 1:1,000 dilution), polyclonal anti P-AMPKα (Thr172) (Cell Signaling- 1:1,000 dilution), polyclonal anti AMPKα (Cell Signaling- 1:1,000 dilution), monoclonal anti IRS1 (Cell Signaling- 1:500 dilution), monoclonal anti P-IRS1 (Tyr612) (Invitrogen- 1:1,000 dilution), monoclonal anti P-IRS1 (Ser307) (Cell Signaling- 1:1,000 dilution), monoclonal anti FAS (Santa Cruz Biotechnology- 1:1,000 dilution), monoclonal anti SREBP1c (Santa Cruz Biotechnology- 1:1,000 dilution), monoclonal anti SPOT14 (Santa Cruz Biotechnology- 1:1,000 dilution), polyclonal anti PGC1α (Millipore- 1:1,000 dilution), monoclonal anti Mfn2 (Abcam- 1:1,000 dilution), monoclonal anti DRP1 (Cell Signaling- 1:1,000 dilution), monoclonal anti Catalase (Merck- 1:750 dilution), monoclonal anti SOD-2 (Santa Cruz Biotechnology- 1:500 dilution), polyclonal anti GPX1 (GeneTex- 1:1,000 dilution), monoclonal anti GPX4 (Abcam- 1:1,000 dilution), polyclonal anti PRDX-3 (Abcam- 1:1,000 dilution) and monoclonal anti B-ACTIN antibody (Bioss Antibodies- 1:1,000 dilution). As secondary antibodies we used peroxidase anti rabbit IgG (Vector Laboratories- 1:2,000–1:4,000–1:10,000 dilution) and peroxidase anti-mouse IgG (Vector Laboratories- 1:4,000 dilution). Horseradish peroxidase-conjugated secondary antibodies were used for signal detection by enhanced chemiluminescence using the Chemi Doc system and related software (Hercules, CA, United States of America).
Petito G., Giacco A., Cioffi F., Mazzoli A., Magnacca N., Iossa S., Goglia F., Senese R, & Lanni A. (2023). Short-term fructose feeding alters tissue metabolic pathways by modulating microRNAs expression both in young and adult rats. Frontiers in Cell and Developmental Biology, 11, 1101844.
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Publication 2023
Actins anti-IgG Antibodies Catalase Chemiluminescence Cloning Vectors Gels Horseradish Peroxidase IRS1 protein, human Liver Milk, Cow's mitofusin 2 protein, human Monoclonal Antibodies Mus Nitrocellulose Peroxidase Phospholipid Hydroperoxide Glutathione Peroxidase PPARGC1A protein, human Proteins PTPN1 protein, human Rabbits SDS-PAGE Serum Albumin, Bovine Signal Detection (Psychology) Sirtuins Skeletal Muscles Sterol Regulatory Element Binding Protein 1c Technique, Dilution THRSP protein, human Tissue, Membrane Tween 20
2
Antioxidant and Metabolic Pathways Evaluation
General reagents were of the highest available grade and were obtained from Sigma Chemical (St. Louis, MO, United States). Precision Plus Protein™ All Blue Prestained Protein Standards were obtained from BioRad (Hercules, CA, United States). Anti-PTP1B, anti-Total OXPHOS complexes cocktail, anti-SIRT-1, anti-Mfn2, anti-GPX4, and anti-PRDX3 antibodies were acquired from Abcam (Cambridge, CA, UK). Anti-P-IRS1 (Ser307), anti-P-AMPKα (Thr172), anti-AMPK-Tot, anti-IRS1, and anti-DRP1 antibodies were obtained from Cell Signaling (Danvers, MA, United States). Anti-P-IRS1(Tyr612) antibody was obtained from Invitrogen (Carlsbad, CA, United States). Anti-FAS, anti-SPOT14, anti-SREBP1c and anti-SOD-2 antibodies were acquired from Santa Cruz Biotechnology (Dallas, TX, United States). Anti-Catalase antibody was obtained from Merck (Darmastadt, DE). Anti-GPX1 antibody was acquired from GeneTex (Irvine, CA, United States). Anti-B-ACTIN antibody was obtained from Bioss Antibodies (Woburn, MA, United States). Secondary antibodies, peroxidase anti-rabbit IgG and peroxidase anti-mouse IgG were obtained from Vector Laboratories (Burlingame, CA, United States). To detect IL-1B, TGF-B, TNF-A, and IL-6 serum levels we used the Enzyme-Linked Immunosorbent Assay-ELISA kits obtained from Invitrogen (Carlsbad, CA, United States). To detect Adiponectin serum levels we used an Enzyme-Linked Immunosorbent Assay-ELISA kits, acquired from Abcam (Cambridge, CA, UK). To detect 8-OHdG we used a DNA/RNA Oxidative Damage ELISA kit obtained from Cayman Chemical Company (Ann Arbor, MI, United States). QIAzol lysis buffer and miRNeasy micro kit were obtained from Qiagen (Hilden, Germany). To synthesize cDNA strands from RNA we used the SuperScript IV reverse Transcriptase for RT-PCR obtained from Invitrogen (Carlsbad, CA, United States). IQ SYBR Green supermix was obtained from Bio-Rad (Hercules, CA, United States). TaqMan® miRNA assays for miR-122-5p, miR-34a-5p, miR-125b-5p, and RNU6B were acquired from Applied Biosystems (Foster City, CA, United States).
Petito G., Giacco A., Cioffi F., Mazzoli A., Magnacca N., Iossa S., Goglia F., Senese R, & Lanni A. (2023). Short-term fructose feeding alters tissue metabolic pathways by modulating microRNAs expression both in young and adult rats. Frontiers in Cell and Developmental Biology, 11, 1101844.
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Publication 2023
8-Hydroxy-2'-Deoxyguanosine Actins Adiponectin Anti-Antibodies anti-IgG Antibodies Antibodies, Anti-Idiotypic Biological Assay Buffers Caimans Catalase Cloning Vectors DNA, A-Form DNA, Complementary Enzyme-Linked Immunosorbent Assay Immunoglobulins IRS1 protein, human MicroRNAs mitofusin 2 protein, human Mus Oxidative Damage Peroxidase Phospholipid Hydroperoxide Glutathione Peroxidase Proteins PTPN1 protein, human Rabbits Reverse Transcriptase Polymerase Chain Reaction Serum Sirtuins Sterol Regulatory Element Binding Protein 1c SYBR Green I THRSP protein, human TNF protein, human Transforming Growth Factor beta
3
Evaluating α-MG's Impact on Liver
Sandwich-ELISA kits for ACC, SREBP-1c, IRS-1, and PI3K (MyBioSource catalog #MBS8303295, #MBS940898, #MBS774816, and #MBS260381, USA, respectively) were performed to analyze the effect of α-MG on liver tissues.
Soetikno V., Andini P., Iskandar M., Matheos C.C., Herdiman J.A., Kyle I.K., Suma M.N., Louisa M, & Estuningtyas A. (2023). Alpha-Mangosteen lessens high-fat/high-glucose diet and low-dose streptozotocin induced-hepatic manifestations in the insulin resistance rat model. Pharmaceutical Biology, 61(1), 241-248.
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Publication 2023
Enzyme-Linked Immunosorbent Assay IRS1 protein, human Liver Phosphatidylinositol 3-Kinases Sterol Regulatory Element Binding Protein 1c Tissues
4
Hepatic Protein Expression Analysis
Liver and isolated hepatocytes were homogenized in RIPA buffer containing a protease inhibitor cocktail (Thermo Scientific, Waltham, MA, USA) and incubated on ice for 10 min. After centrifuging for 15 min at a maximum speed, the protein concentration of the supernatant was determined using the PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific). Twenty micrograms of liver protein or 5 μg of hepatocyte protein were used for immunoblotting with primary antibodies for fatty acid translocase (FAT/CD36) (AF2519, R&D Systems, Minneapolis, MN, USA), fatty acid binding protein 1 (FABP1) (ab7366, Abcam), fatty acid synthase (FASN) (3180, Cell Signaling Technology, Beverly, MA, USA), acetyl-CoA carboxylase (ACC) (3676, Cell Signaling Technology), insulin receptor (IR) (3025, Cell Signaling Technology), PY99 (p-Tyr, 7020, Santa Cruz Biotechnology, Dallas, TX, USA), insulin receptor substrate-1 (IRS-1) (611394, BD Bioscience, Franklin Lakes, NJ, USA), phosphatidylinositol-3-kinase (PI3-K) (610045, BD Bioscience), sterol regulatory element-binding protein-1c (SREBP-1c) (NB600-582, NB100-2215, Novus Biologicals, Centennial, CO, USA), protein kinase B (Akt) (9272, Cell Signaling Technology), phospho-Akt (Ser473, 9271, Cell Signaling Technology), mammalian target of rapamycin (mTOR) (2983, Cell Signaling Technology), phospho-mTOR (Ser2448, 2971, Cell Signaling Technology), AMP-activated protein kinase α (AMPKα) (2603, Cell Signaling Technology), phospho-AMPKα (Thr172, 2535, Cell Signaling Technology), or β-Actin (A5441, Sigma-Aldrich, St. Louis, MO, USA), at dilutions suggested by the manufacturers. Densitometric analysis was performed by ImageQuantTM LAS 4000 (GE Healthcare Bioscience, Piscataway, NJ, USA), and quantification values were normalized against β-Actin.
Tajima O., Fujita Y., Ohmi Y., Furukawa K, & Furukawa K. (2023). Ganglioside GM3 prevents high fat diet-induced hepatosteatosis via attenuated insulin signaling pathway. PLOS ONE, 18(2), e0281414.
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Publication 2023
1-Phosphatidylinositol 3-Kinase Acetyl-CoA Carboxylase Actins AKT1 Protein Kinase AMP-Activated Protein Kinases Antibodies Biological Assay Biological Factors Buffers Densitometry FABP1 protein, human FASN protein, human Fatty Acids FRAP1 protein, human Hepatocyte Insulin Receptor Insulin Receptor Substrate-1 Protein Liver Novus Protease Inhibitors Protein Kinases Proteins Radioimmunoprecipitation Assay Sterol Regulatory Element Binding Protein 1c Technique, Dilution
5
Macrophage Differentiation and Gene Expression
U937 cell line was differentiated in foam macrophages with phorbol 12-myristate 13-acetate (PMA) 10 ng/ml (Sigma) for 72 hours at 37 °C in RPMI 10% FBS. At day 3 T0901317 (10 μM) or different amount of PFM037 (50, 25 and 10 μM) were added for 16 hours. Total RNA was purified by TRIZOL (Invitrogen). Reverse transcription was performed by MLV-reverse transcriptase (Promega). QPCR was performed using SYBR Green Master Mix (Applied Biosystems) and real-time PCR (Viia 7 Real Time PCR System, Applied Biosystems). PCR reactions were done in triplicate. The comparative Ct method was used to quantify transcripts that were normalized for human GAPDH. We used the following primer pairs:
GAPDH-F 5’-ACA TCA TCC CTG CCT CTA CTG-3’; GAPDH-R 5’-ACC ACC TGG TGC TCA GTG TA-3’
ABCA1-F 5’-CCA GGC CAG TAC GGA ATT C-3’; ABCA1-R 5’-CCT CGC CAA ACC AGT AGG A-3’
SREBP-1c-F 5’-GGC GGG CGC AGA TC-3’; SREBP-1c-R 5’-TTG TTG ATA AGC TGA AGC ATG TCT-3’
FAS-F 5’-ACA GCG GGG AAT GGG TAC T-3’; FAS-R 5’-GAC TGG TAC AAC GAG CGG AT-3’
SCD1-F 5’-TTC AGA AAC ACA TGC TGA TCC TCA TAA TTC-3’; SCD1-R 5’-ATT AAG CAC CAC AGC ATA TCG CAA GAA AGT-3’
Raccosta L., Marinozzi M., Costantini S., Maggioni D., Ferreira L.M., Corna G., Zordan P., Sorice A., Farinello D., Bianchessi S., Riba M., Lazarevic D., Provero P., Mack M., Bondanza A., Nalvarte I., Gustafsson J.A., Ranzani V., De Sanctis F., Ugel S., Baron S., Lobaccaro J.M., Pontini L., Pacciarini M., Traversari C., Pagani M., Bronte V., Sitia G., Antonson P., Brendolan A., Budillon A, & Russo V. (2023). Harnessing the reverse cholesterol transport pathway to favor differentiation of monocyte-derived APCs and antitumor responses. Cell Death & Disease, 14(2), 129.
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Publication 2023
ABCA1 protein, human Agkistrodon contortrix contortrix protein C activator Cell Lines fluorinated trisacryl conjugate Foam Cells GAPDH protein, human Homo sapiens Oligonucleotide Primers Promega Real-Time Polymerase Chain Reaction Reverse Transcription RNA-Directed DNA Polymerase Sterol Regulatory Element Binding Protein 1c SYBR Green I T0901317 Tetradecanoylphorbol Acetate trizol U937 Cells

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1
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Srebp 1c by Santa Cruz Biotechnology
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SREBP-1c is a protein that acts as a transcription factor, regulating the expression of genes involved in lipid and cholesterol biosynthesis. It plays a central role in the control of lipid metabolism.
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Rneasy mini kit by Qiagen
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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β actin by Santa Cruz Biotechnology
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β-actin is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells. It is a component of the microfilament system and plays a crucial role in various cellular processes, such as cell motility, maintenance of cell shape, and intracellular trafficking.
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TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
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Srebp 1c by Abcam
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SREBP-1c is a transcription factor that plays a key role in the regulation of genes involved in lipid and cholesterol metabolism. It is a member of the sterol regulatory element-binding protein (SREBP) family.
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Srebp 1c by Cell Signaling Technology
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SREBP-1c is a recombinant protein that represents the mature nuclear form of the Sterol Regulatory Element Binding Protein 1C. It is a transcription factor that regulates genes involved in lipid and cholesterol biosynthesis.
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The High-Capacity cDNA Reverse Transcription Kit is a laboratory tool used to convert RNA into complementary DNA (cDNA) molecules. It provides a reliable and efficient method for performing reverse transcription, a fundamental step in various molecular biology applications.
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β actin by Cell Signaling Technology
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β-actin is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells. It is an important component of the microfilament system and is involved in various cellular processes such as cell motility, structure, and integrity.

More about "Sterol Regulatory Element Binding Protein 1c"

Sterol Regulatory Element Binding Protein 1c (SREBP-1c) is a pivotal transcription factor that plays a crucial role in regulating lipid and cholesterol metabolism.
As a member of the SREBP family, SREBP-1c is known to activate genes involved in the synthesis of fatty acids, triglycerides, and cholesterol.
This isoform is predominantly expressed in the liver and adipose tissue, and its activity is modulated by various nutritional and hormonal signals.
Dysregulation of SREBP-1c has been implicated in the development of metabolic disorders such as non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes.
Understanding the regulatory mechanisms and functions of SREBP-1c is crucial for developing targeted therapies for these conditions.
Researchers often utilize techniques like TRIzol reagent and the RNeasy Mini Kit to extract and purify RNA samples, including SREBP-1c mRNA.
Quantitative analysis of SREBP-1c expression can be performed using real-time PCR with β-actin as a reference gene.
Western blotting with PVDF membranes can also be used to assess SREBP-1c protein levels and activation.
The High-Capacity cDNA Reverse Transcription Kit is commonly used to convert extracted RNA into cDNA for downstream applications.
Understanding the role of SREBP-1c and its regulatory pathways is crucial for unraveling the mechanisms underlying metabolic disorders and developing innovative therapies.
Researchers in this field continue to explore the complexities of SREBP-1c function and its potential as a therapeutic target.