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Substance P

Substance P is a neuropeptide that plays a key role in pain perception, inflammation, and neurogenic processes.
It is a member of the tachykinin family and is primarily found in the central and peripheral nervous systems.
Substance P acts as a neurotransmitter and neuromodulator, mediating a wide range of physiological functions, including vasodilation, smooth muscle contraction, and stimulation of the release of other neurotransmitters.
It is also implicated in the pathogenesis of various diseases, such as migraine, asthma, and chronic pain conditions.
Substance P research is crucial for understanding its mechanims of action and developing targeted theraputic interventions.
PubCompare.ai can help optimize your Substance P research by providing access to the most reliable protocols and identifying the best methods and products through AI-driven comparisons, enhancing reproducibility and accuracy.

Most cited protocols related to «Substance P»

1
Immunohistochemistry for Renal Denervation
Cited 13 times

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Publication 2014
Anabolism Arteries Calcitonin Gene-Related Peptide Catecholamines Cells Denervation Dissection Dopa Dopa Decarboxylase Dopamine Elastica Elastic Fibers Enzymes Ganglia Glial Fibrillary Acidic Protein High Blood Pressures Kidney Nerve Fibers Nervousness Neurofilament Proteins Neuroglia Neurons Neurotransmitters Norepinephrine S100 Proteins Substance P Tissues trichrome stain Tyrosine Tyrosine 3-Monooxygenase
2
Coronary Arteriole Vasodilation Mechanisms
Cited 11 times
Coronary arterioles (80- to 180-μm internal diameters) were dissected from the right atria appendage pre- and post-CP Rep. Microvessel studies were performed by in vitro organ bath video-microscopy as described previously.3 (link),6 (link) After a 60-minute stabilization period in the organ chamber, the microvessels were preconstricted with thromboxane A2 analog U46619×30% to 40% of the baseline diameter. After achievement of this constricted steady state, dose-dependent relaxation was measured in response to (extraluminal) application of the following vasodilators: the activator of intermediate and small conductance KCa channels (IKCa/SKCa), NS309, the activator of large conductance KCa channels (BKCa), NS1619 (both from 10-9 to 10-5 M), sodium nitroprusside (SNP), adenosine 5′-diphosphate (both from 10-9 to 10-4 M), and substance P (10-12 to 10-7 M). After exposure to substance P, the vessel was discarded to avoid tachyphylaxis. One or 3 interventions were performed on each vessel. The order of drug administration was random. Six vessels harvested before CP Rep were pretreated with a mixture of the IKCa blocker, TRAM34 (10-7 M), and the SKCa blocker, apamin (10-6 M), before perfusion with NS309. Six additional vessels were pretreated with the BKCa blocker, iberiotoxin (10-7 M) before perfusion with NS1619. In some cases, endothelial denudation was carried out by advancing a human hair into the lumen and gently abrading the luminal surface.
Feng J., Liu Y., Clements R.T., Sodha N.R., Khabbaz K.R., Senthilnathan V., Nishimura K.K., Alper S.L, & Sellke F.W. (2008). Calcium-Activated Potassium Channels Contribute to Human Coronary Microvascular Dysfunction After Cardioplegic Arrest. Circulation, 118(14 Suppl), S46-S51.
Publication 2008
Adenosine Diphosphate Apamin Arterioles Atrium, Right Bath Blood Vessel Endothelium Hair Heart Homo sapiens iberiotoxin Microscopy, Video Microvessels Nitroprusside, Sodium NS 1619 Perfusion Phenobarbital Substance P Tachyphylaxis Thromboxane A2 U-44619 Vasodilator Agents
3
Visualizing hM4Di DREADD Expression
Cited 10 times
hM4Di receptor expression in cell bodies and processes was visualized with immunohistochemistry for HA, GFP, or mCherry tags (described below). Containment of transduction sites within RVP or CVP, or VTA or SN dopamine cells, was confirmed by co-staining for substance P (SP; defining borders of VP), or TH (defining VTA and SN), and a brain atlas50 . A synapsin promoter virus was employed in VP, so some expression was observed outside the borders of RVP or CVP in most animals. Animals with more than 30% of DREADD expression observed outside RVP or CVP were excluded from analyses (n=7). For animals with RVP virus injections, no more than 20% of DREADD-expressing tissue encroached into CVP (i.e., caudal of bregma), or vice versa. Animals injected with the Syn-GFP control virus in VP had comparable GFP expression patterns to hM4Di animals. For TH::Cre rats, hM4Di expression was robust in either VTA or SN, and was restricted almost exclusively to TH+ cell bodies and processes [% mCherry+ neurons coexpressing TH: m±SEM=97.5±0.7%; Fig. 6b–c, Supplemental Fig. 6]. mCherry expression was not observed in the contralateral hemisphere, nor did expression extend substantially into the SN of VTA-injected animals, or into VTA of SN-injected animals (Fig. 6b–c).
Mahler S.V., Vazey E.M., Beckley J.T., Keistler C.R., McGlinchey E.M., Kaufling J., Wilson S.P., Deisseroth K., Woodward J.J, & Aston-Jones G. (2014). Designer Receptors Show Role for Ventral Pallidum Input to Ventral Tegmental Area in Cocaine Seeking. Nature neuroscience, 17(4), 577-585.
Publication 2014
Animals Animal Viruses Brain Cell Body Cells Dopamine Immunohistochemistry Neurons Rattus norvegicus Substance P Synapsins Tissues Virus
4
Immunohistochemistry of Tendon Pathology
Cited 10 times
Immunohistochemical analysis was performed on flexor digitorum tendons collected from normal controls (n=12), trained controls (n = 9), and rats that had performed either the HRHF task for 3 (n = 4), 6 (n=4) or 12 weeks (n=4), or the LRNF task for 3 (n=3), 6 (n=4) or 12 weeks (n = 3). Animals were euthanized, perfused transcardially with 4% paraformaldehyde in PO4 buffer, and forearm musculotendinous tissues were dissected as a mass off the forearm bones as shown in Figure 3K,L, and sectioned longitudinally as a soft tissue mass (en bloc) as described previously (19 (link), 23 (link)). Sections, on slides, were incubated in 3% H2O2 in methanol (4°C) for 30 min, washed, incubated in 4% dried milk in PBS (Blotto) for 20 min, and then overnight at rm temp with a Substance P antibody (# MAB1566, Chemicon, Temecula, CA; 1:500 dilution with 4% carnation milk in PBS). After washing, sections were incubated for 2 hrs at rm temp with goat anti-mouse peroxidase-conjugated (HRP) secondary antibody (Jackson ImmunoResearch, West Grove, PA) diluted 1:100 with PBS. HRP was visualized as a black immunoreactive stain using diaminobenzidene (DAB) with cobalt (Sigma-Aldrich, St. Louis, MO). For IL-1β and periostin like factor (PLF; labels activated fibroblasts producing this matricellular protein), sections were immunolabeled and detected with HRP-DAB as previously described (21 (link),24 (link)). Eosin and/or nuclear red were used as counterstains. A series of adjacent sections were also stained with hematoxylin and eosin (H&E) only. Sections were dehydrated and coverslipped with DPX mounting medium. For connective tissue growth factor (CTGF; a fibroblast growth factor that induces collagen production and an activated fibroblast marker), sections were immunolabeled and detected with Cy3 (red fluorescence), and coverslipped with 80% glycerol in PBS, as previously described (22 (link)). Negative control slides included omission of either the primary antibody or the secondary antibody.
Selected sections were double-labeled after Substance P immunolabeling with either anti-ED1 (detects a 90 kDa lysosomal membrane protein in monocytes/macrophages) or anti-PGP9.5 (a pan neuronal marker). After Substance P immunolabeling with secondary antibody conjugated to Cy2 (green tag; Jackson, diluted 1:100 in PBS for 2 hrs), tissue sections were washed, digested with 0.5% pepsin in 0.01 N HCl for 20 min at rm temp, and then incubated with goat serum (4%) in PBS for 30 min at rm temp. Sections were then incubated with either anti-ED1 (MAB1435, Chemicon, Temecula, CA, 1:250 dilution in 4% goat serum in PBS) or anti-PGP9.5 (ab8189, Abcam, Cambridge, MA, 1:50 dilution in 10% goat serum in PBS) overnight at rm temp. Sections were incubated with appropriate secondary antibodies conjugated to Cy3 (red tag; Jackson). Slides were coverslipped with 80% glycerol in PBS. Selected sections were also double-labeled with CTGF and collagen type I antibodies as described previously (22 (link)).
Fedorczyk J.M., Barr A.E., Rani S., Gao H., Amin M., Amin S., Litvin J, & Barbe M.F. (2010). Exposure dependent increases in IL-1beta, Substance P, CTGF and tendinosis in flexor digitorum tendons with upper extremity repetitive strain injury. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(3), 298-307.
Publication 2009
Animals Antibodies Buffers Carnation Cobalt Collagen Collagen Type I Connective Tissue Growth Factor Eosin Fibroblast Growth Factor Fibroblasts Fluorescence Forearm Glycerin Goat Immunoglobulins Interleukin-1 beta Lysosome-Associated Membrane Glycoproteins Macrophage Methanol Milk, Cow's Monocytes Mus Neurons paraform Pepsin A Peroxidase Peroxide, Hydrogen POSTN protein, human Proteins Rattus Serum Stains Substance P Technique, Dilution Tendons Tissues UCHL1 protein, human Ulna
5
Visualizing hM4Di DREADD Expression
Cited 9 times
hM4Di receptor expression in cell bodies and processes was visualized with immunohistochemistry for HA, GFP, or mCherry tags (described below). Containment of transduction sites within RVP or CVP, or VTA or SN dopamine cells, was confirmed by co-staining for substance P (SP; defining borders of VP), or TH (defining VTA and SN), and a brain atlas50 . A synapsin promoter virus was employed in VP, so some expression was observed outside the borders of RVP or CVP in most animals. Animals with more than 30% of DREADD expression observed outside RVP or CVP were excluded from analyses (n=7). For animals with RVP virus injections, no more than 20% of DREADD-expressing tissue encroached into CVP (i.e., caudal of bregma), or vice versa. Animals injected with the Syn-GFP control virus in VP had comparable GFP expression patterns to hM4Di animals. For TH::Cre rats, hM4Di expression was robust in either VTA or SN, and was restricted almost exclusively to TH+ cell bodies and processes [% mCherry+ neurons coexpressing TH: m±SEM=97.5±0.7%; Fig. 6b–c, Supplemental Fig. 6]. mCherry expression was not observed in the contralateral hemisphere, nor did expression extend substantially into the SN of VTA-injected animals, or into VTA of SN-injected animals (Fig. 6b–c).
Mahler S.V., Vazey E.M., Beckley J.T., Keistler C.R., McGlinchey E.M., Kaufling J., Wilson S.P., Deisseroth K., Woodward J.J, & Aston-Jones G. (2014). Designer Receptors Show Role for Ventral Pallidum Input to Ventral Tegmental Area in Cocaine Seeking. Nature neuroscience, 17(4), 577-585.
Publication 2014
Animals Animal Viruses Brain Cell Body Cells Dopamine Immunohistochemistry Neurons Rattus norvegicus Substance P Synapsins Tissues Virus

Most recents protocols related to «Substance P»

1
Quantifying Inflammatory Mediators in Rat
The levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, prostaglandin E2 (PGE2), and IL-10 in rat serum and substance P (SP), hyaluronic acid (HA), bradykinin (BK), and prostacyclin (PGI2) in rat skin tissues (corresponding to the L6 DRG) were examined by quantizing enzyme-linked immunosorbent assay (ELISA) kit (ZhuoCai Biological Technology, China) based on the manufacturer's instructions. The absorbance of wells was measured with a microplate reader (SpectraMAX Plus384, USA) at 450 nm wavelength to calculate the sample concentration.
Wang Y.L., Zhu H.Y., Lv X.Q., Ren X.Y., Peng Y.C., Qu J.Y., Shen X.F., Sun R., Xiao M.L., Zhang H., Chen Z.H, & Cong P. (2023). Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation. Neural Plasticity, 2023, 9303419.
Full text: Click here
Publication 2023
Biopharmaceuticals Bradykinin Dinoprostone Enzyme-Linked Immunosorbent Assay Epoprostenol Hyaluronic acid IL1B protein, human Interleukin-6 Interleukin-10 Serum Skin Substance P TNF protein, human
2
Biomarker Quantification in Livestock Research
PC and SC levels were analyzed using a SC immunoassay kit according to the specification of kits (Salimetrics LLC, State College, PA, USA). The value for intra- and inter-assay coefficient of variability (CV) of PC was 6.64% and 9.85%, respectively. The intra-assay and inter-assay CV values of SC were 8.84% and 9.13%, respectively. Plasma SP was analyzed using a substance P ELISA kit (Enzo Life Sciences, Farmingdale, NY, USA). The intra-assay CV was 8.42%, whereas the intra-assay CV was 10.6%. Plasma haptoglobin concentrations were analyzed using a bovine haptoglobin enzyme immunoassay kit (GenWay Biotech, San Diego, CA, USA). The intra- and inter-assay CV values were 1.46% and 8.67%, respectively. The analytical methods were verified in our previous study (Park et al [3 (link)]).
Yoo S., Beak S.H., Kang H.J., Jung D.J., Fassah D.M., Jeong I., Park S.J., Haque M.N., Kim M, & Baik M. (2023). Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves. Animal Bioscience, 36(3), 521-528.
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Publication 2023
Biological Assay Cattle Enzyme-Linked Immunosorbent Assay Enzyme Immunoassay Haptoglobins Immunoassay Plasma Substance P
3
Spatio-temporal Protease Dynamics Mapping
Erich et al. cut consecutive sections from five different porcine tissues (kidney, spleen, pancreas, liver and muscle) and spotted four times 1 μl protease inhibitor mix onto each tissue section19 . For each organ, five tissue sections were spray-covered with substance P before incubation to allow its digestion in the areas without protease inhibitor mix (‘digested samples’) and the other six sections were spray-covered after incubation to prevent digestion (‘not digested samples’). Incubation times were set to 15, 30, 60, 120 and 360 min to measure the endogenous protease activity over time. Afterwards, imaging with a MALDI-TOF device in the m/z range 500 to 2500 at 200 μm spatial resolution was performed. The 55 GB imzML ‘time-curve-dataset’ was downloaded from PRIDE (PXD011104) and imported into Cardinal. Peak detection was performed on the mean spectrum with the MAD noise estimation and a signal to noise ratio of 2.5. Peaks were aligned to the mean spectrum and only m/z features with non-zero mean intensities were kept. The peak area was integrated in the total ion current normalized data at the m/z peak positions ± 100 ppm. Time and memory consumption for these calculations were recorded once while using a single core and once with two cores via the BiocParallel function. Next, the ion image of substance P (m/z 1347.7) was plotted on the preprocessed file with the following parameters: contrast enhancement via histogram and adaptive image smoothing. All tissue specimens were annotated for their condition (digest/no digest) and time points (15, 30, 45, 60, 120, 360 min) via their position in the x-y coordinate grid. The five different tissue types were manually annotated according to the annotation provided in the original manuscript. These spectra annotations were integrated with the MSI data and filtered for ‘digest’ and ‘no digest’ spleen spectra. Substance P (m/z 1347.7) was plotted in the ‘digest’ spleen tissues with contrast enhancement via suppression and Gaussian image smoothing. Single ion segmentation15 (link) of substance P in the digested spleen was performed to obtain the spectra with and without inhibitor mix. This was performed by applying spatially-aware Dirichlet Gaussian mixture model (DGMM) with a radius (r) of one, two clusters (k) and without annealing during parameter optimization. The obtained spatial clusters were plotted. For both clusters, substance P mean intensity was calculated for the digested (no inhibitor mix) and not digestion (inhibitor mix) spectra of both datasets at all digestion times. The obtained mean intensities were normalized to the mean intensity of substance P in the undigested spleen tissues at the same time points, log transformed and plotted as a time curve.
Bemis K.A., Föll M.C., Guo D., Lakkimsetty S.S, & Vitek O. (2023). Cardinal v3 - a versatile open source software for mass spectrometry imaging analysis. bioRxiv.
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Publication Preprint 2023
Acclimatization Digestion Histocompatibility Testing Ion Transport Kidney Liver Medical Devices Memory Muscle Tissue Pancreas Peptide Hydrolases Pigs Protease Inhibitors Radius SERPINA1 protein, human Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spleen Substance P Tissues
4
Gene Expression Analysis of DRG Neurons
In accordance with our previous study [10 (link)], rat L1 to L3 DRGs were harvested to analyze the gene expression of substance P (SP) and calcitonin gene-related peptide (CGRP), because bilateral L1–L3 DRGs innervate the lower IVDs [14 (link)]. In brief, the TRIzol reagent (Invitrogen, Life Technologies Corporation, CA, USA) was used to extract the total RNA, and the cDNA was produced with reverse transcriptase kit (TaKaRa, Shiga, Japan). The next qRT-PCR was conducted using SYBR Premix Ex Taq Kit (TaKaRa, Shiga, Japan) in the ABI 7500 Sequencing Detection System (Applied Biosystems, CA, USA). In this process, the reaction condition was set as follows: 40 cycles of denaturation at 95°C for 5 s and amplification at 60°C for 24 s. In this study, the primer sequences were designed and used: rat GAPDH: forward 5′-ATGACTCTACCCACGGCAAG-3′ and reverse 5′-TACTCAGCACCAGCATCACC-3′; rat SP: forward 5′-TGGTCAGATCTCTCACAAAGG-3′ and reverse 5′-TGCATTGCGCTTCTTTCATA-3′; rat CGRP: forward 5′-TCTAGTGTCACTGCCCAGAAGAGA-3′ and reverse 5′-GGCACAAAGTTGTCCTTCACCACA-3′; human GAPDH: forward 5′-CAGGAGGCATTGCTGATGAT-3′ and reverse 5′-GAAGGCTGGGGCTCATTT-3′; and human NGF: forward 5′-GCAAGCGGTCATCATCCCATCC-3′ and reverse 5′-TCTGTGGCGGTGGTCTTATCCC-3′.
All reactions had triplicate repeat and the housekeeping gene was GAPDH. To normalize the targeted gene expression, the 2-△△Ct method was used when compared with the gene expression of GAPDH.
Li J., Duan W., Chai S., Luo Y., Ma Y., Yang N., Liu M, & He W. (2023). Wogonin, a Bioactive Ingredient from Huangqi Guizhi Formula, Alleviates Discogenic Low Back Pain via Suppressing the Overexpressed NGF in Intervertebral Discs. Mediators of Inflammation, 2023, 4436587.
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Publication 2023
Adjustment Disorders Calcitonin Gene-Related Peptide DNA, Complementary GAPDH protein, human Gene Expression Genes, Housekeeping Homo sapiens Oligonucleotide Primers RNA-Directed DNA Polymerase Substance P trizol
5
Histological Analysis of Prostate and Bladder
The prostate and the bladder were harvested 14 days after saline or CAR injection, fixed in 10% formaldehyde buffer for 24–48 h, and then embedded in paraffin for H&E staining and immunohistochemistry.
The prostate and the bladder tissue sections for H&E staining and immunohistochemistry were cut into 3 μm sections, de-paraffinized in xylene for 10 min, and rehydrated with gradient descent ethanol at room temperature. Sections were stained in Gill II hematoxylin solution (Leica, Wetzlar, Germany, 3801522) for 1 min. Counterstaining was conducted in Eosin Y solution (Leica, 3801602) for 5 min. The xylene-based mounting agent was found to seal the coverslip. The inflammatory reaction of CAR-induced cystitis was graded on a score of 0–3 as follows: 0, no evidence of inflammatory infiltration or interstitial edema; 1, mild (few inflammatory cell infiltrates and little or no interstitial edema); 2, moderate (moderate amount of inflammatory cell infiltrates and moderate interstitial edema); 3, severe (diffuse presence of large amounts of inflammatory cell infiltrates and severe interstitial edema) [37 (link),38 (link)].
Moreover, 3% hydrogen peroxide was used for 10 min to block endogenous peroxidase activity. Tissue sections were incubated with anti-Drp-1 (1:2000 dilution; Abcam, Cambridge, UK), anti-mitofusin 2 (MFN-2) (1:25 dilution; Abcam), anti-NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) (1:200 dilution; Invitrogen, Waltham, MA, USA), anti-substance P (1:200 dilution; Invitrogen), and anti-CGRP-receptor component protein (CGRP-RCP) (1:200 dilution; Abcam) at 37 °C for 60 min, and then washed twice with PBS buffer. Then, we applied the Primary Antibody Amplifier Quanto (Waltham, MA, USA) and incubated for 10 min. After that, we applied the HRP Polymer Quanto (Waltham, MA, USA) and incubated for 10 min. After washing in PBS, we added 30 µL of DAB Quanto Chromogen (Waltham, MA, USA) to 1 mL DAB Quanto Substrate (Waltham), mixed, and applied to the tissue. We incubated for 5 min. Then, we used a xylene-based mounting agent for coverslip sealing. IHC staining scores were calculated based on the proportion of positively stained areas in tissue section images. Three randomly selected fields were analyzed using Image-Pro Plus version 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA).
Wu Z.S., Wang H.J., Lee W.C., Luo H.L., Lin T.K, & Chuang Y.C. (2023). Low-Energy Shock Wave Suppresses Prostatic Pain and Inflammation by Modulating Mitochondrial Dynamics Regulators on a Carrageenan-Induced Prostatitis Model in Rats. International Journal of Molecular Sciences, 24(4), 3898.
Full text: Click here
Publication 2023
azo rubin S Buffers Calcitonin-Gene Related Peptide Receptor Cardiac Arrest Cells Cystitis Edema Eosin Ethanol Formaldehyde Gills Immunoglobulins Immunohistochemistry Inflammation mitofusin 2 protein, human Paraffin Embedding Peroxidase Peroxide, Hydrogen Polymers Prostate Proteins Pyrin Domain Saline Solution Substance P Technique, Dilution Tissues Tissue Stains Urinary Bladder Xylene

Top products related to «Substance P»

1
Substance p by Merck Group
Sourced in United States, United Kingdom
Substance P is a laboratory reagent used for research purposes. It is a neuropeptide that functions as a neurotransmitter or neuromodulator in the central and peripheral nervous systems. Substance P is involved in the transmission of pain and other sensory information.
2
Substance p by Bio-Techne
Sourced in United Kingdom
Substance P is a neuropeptide that functions as a neurotransmitter and neuromodulator in the central and peripheral nervous systems. It is involved in the transmission of pain signals and the regulation of various physiological processes. Substance P is commonly used in research applications to study its role in neurological and biological processes.
3
Substance p by AnaSpec
Sourced in United States
Substance P is a neuropeptide that functions as a neurotransmitter and neuromodulator. It is involved in the transmission of pain and inflammatory responses in the nervous system.
4
Bradykinin by Merck Group
Sourced in United States, Germany, United Kingdom, Canada, Brazil, Australia, France
Bradykinin is a lab equipment product manufactured by Merck Group. It is a peptide that plays a role in the regulation of blood pressure and inflammation. Bradykinin functions by interacting with specific receptors on cell surfaces.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
7
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
8
Acth clip 18 39 by Bruker
Sourced in Germany
The ACTH clip 18–39 is an analytical standard used for the identification and quantification of adrenocorticotropic hormone (ACTH) in biological samples. It is a synthetic peptide fragment corresponding to the amino acid sequence 18 to 39 of the ACTH protein. This product is intended for research use only and is not for use in diagnostic procedures.
9
Substance p acetate salt hydrate by Merck Group
Sourced in United States
Substance P acetate salt hydrate is a chemical compound used in laboratory research. It is a salt form of the neuropeptide Substance P, which plays a role in various physiological processes. The product is available in a hydrated state.
10
Substance p by Bachem
Sourced in Switzerland
Substance P is a laboratory product manufactured by Bachem. It is a neuropeptide that functions as a neurotransmitter or neuromodulator. Substance P is involved in the transmission of pain signals and the regulation of various physiological processes.

More about "Substance P"

Substance P (SP) is a neuropeptide that plays a crucial role in pain perception, inflammation, and neurogenic processes.
It belongs to the tachykinin family and is primarily found in the central and peripheral nervous systems.
SP acts as both a neurotransmitter and neuromodulator, mediating a wide range of physiological functions, such as vasodilation, smooth muscle contraction, and the stimulation of other neurotransmitter release.
This versatile peptide is also implicated in the pathogenesis of various conditions, including migraine, asthma, and chronic pain disorders.
Alongside SP, related compounds like Bradykinin, a potent vasodilator, and ACTH clip 18–39, a synthetic peptide, have garnered significant research interest.
The use of materials such as FBS (Fetal Bovine Serum) and Bovine Serum Albumin (BSA) are common in SP-related experiments, while TRIzol reagent is often employed for RNA extraction and purification.
Optimizing SP research is crucial for understanding its mechanisms of action and developing targeted therapeutic interventions.
PubCompare.ai, a leading AI-driven platform, can assist researchers in this endeavor by providing access to the most reliable protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best methods and products.
This enhances reproducibility, accuracy, and the overall efficiency of SP research, allowing scientists to make groundbreaking discoveries in this important field of study.