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Thrombopoietin

Thrombopoietin is a glycoprotein hormone that regualtes the production and function of platelets.
It is produced primarily by the liver and kidneys, and acts on megakaryocytes to stimulate their proliferation and differentiation.
Thrombopoietin plays a crucial role in maintaining platelet homeostasis and is an important target for therapeutic interventions related to platelet disorders and thrombotic conditions.
Researchers can leverage PubCompare.ai's innovative AI platform to optimize reproducible protocols and identify the best products for studying thrombopoietin and its role in health and disease.

Most cited protocols related to «Thrombopoietin»

QQ culture medium of Stem Line II (Sigma‐Aldrich, St. Louis, MO) contained the 5 human recombinant proteins: stem cell factor (SCF); thrombopoietin (TPO); Flt‐3 ligand; vascular endothelial growth factor (VEGF); and interleukin (IL)‐6. Then, isolated PBMNCs were cultured for 7 days at the cell density of 2×106 cells/2 mL QQ culture medium per well of 6‐well Primaria tissue culture plate (BD Falcon; BD Biosiences, San Jose, CA). Cell density in QQ culture was corresponded to the approximate density of 1×106 MNCs in 1 mL of PB. Culture well plates and the contents of QQ culture medium are listed in Tables 1 and 2.
Publication 2014
Cell Culture Techniques Cells Culture Media flt3 ligand Interleukin-6 NR4A2 protein, human Stem, Plant Stem Cell Factor Thrombopoietin Tissues Vascular Endothelial Growth Factors

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Publication 2014
Animals Cells Cloning Vectors flt3 ligand Granulocyte Colony-Stimulating Factor Homo sapiens Infection Macaca retronectin Serum Albumin, Human Stem Cell Factor Sulfate, Protamine Thrombopoietin Transplantation
Cryopreserved BM or peripheral blood mononuclear cells were thawed and cultured in X-VIVO 15 media with 1% nonessential amino acids (NEAA), 1 mM L-glutamine and 0.1 mM β-mercaptoethanol (2ME) and supplemented with 100 ng/ml stem cell factor (SCF), 100 ng/ml Flt3 ligand (Flt3L), 100 ng/ml thrombopoietin (TPO) and 20 ng/ml IL-3 for 1–4 days. The excisable lentiviral vector CMV-fSV2A expressing OCT4, KLF4, c-MYC and SOX2 (derived from pLM-fSV2A12 (link) after replacing CMV for hPGK) was used and produced as described13 (link). For induction of reprogramming, 10,000–250,000 cells were plated on retronectin-coated 24-well dishes and transduced at an MOI of 30 or higher for 16h. Two days later, the cells were harvested and plated on mitotically inactivated MEFs (GlobalStem) in 6-well plates and the plates were centrifuged at 500g for 30 min at RT. The next day and every day thereafter, half of the medium was changed to hESC medium with 0.5 mM valproic acid (VPA). Colonies with hPSC morphology were manually picked and expanded, as described13 (link).
Culture of hPSCs on MEFs or in feeder-free conditions was performed as previously described13 (link). Characterization of pluripotency (flow cytometry, OCT4 promoter methylation analysis, teratoma formation assays) and Cre-mediated vector excision were done as previously described12 (link), 13 (link), 47 (link). Patient samples were obtained with informed consent under protocols approved by an Institutional Review Board at Memorial Sloan-Kettering Cancer Center and the Fred Hutchinson Cancer Research Center.
Publication 2015
2-Mercaptoethanol Amino Acids Biological Assay Cells Cloning Vectors Ethics Committees, Research Flow Cytometry flt3 ligand Glutamine Human Embryonic Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal KLF4 protein, human Malignant Neoplasms Methylation Oncogenes, myc Patients PBMC Peripheral Blood Mononuclear Cells POU5F1 protein, human retronectin SOX2 protein, human Stem Cell Factor Teratoma Thrombopoietin Valproic Acid
To evaluate the number of circulating progenitor cells after mobilization, a colony-forming cell assay was employed. Red blood cells (RBCs) were lysed with BD Pharm Lyse buffer (BD Biosciences, San Jose, CA; http://www.bdbiosciences.com). Nucleated cells were subsequently washed twice and used for colony-formation assays. Briefly, cells were resuspended in methylcellulose base media provided by the manufacturer (R&D Systems, Inc., Minneapolis, MN; http://www.rndsystems.com) supplemented with granulocyte macrophage colony-stimulating factor (GM-CSF, 25 ng/ml) plus interleukin-3 (IL-3, 10 ng/ml) for colony forming units (CFU) granulocyte/macrophage (GM), granulocyte colony stimulating factor (G-CSF, 20 ng/ml) for CFU-granulocyte (G), macrophage colony stimulating factor (M-CSF, 10 ng/ml) for CFU macrophage (M), erythropoietin (EPO, 5 unit/ml, Stem cell Tech, http://www.stemcell.) plus stem cell factor (SCF, 5 ng/ml) for burst-forming units (BFU-E), and thrombopoietin (TPO, 100 ng/ml) for CFU-megakaryocytes (Megs). All growth factors were purchased from the same company unless otherwise mentioned. Cultures were incubated for 7 days, at which time they were scored under an inverted microscope for the number of each colonies.
Publication 2010
Biological Assay Buffers Cells Erythrocytes Erythropoietin Granulocyte Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte-Macrophage Colony Forming Units Granulocyte Colony-Stimulating Factor Growth Factor Macrophage Macrophage Colony-Stimulating Factor Megakaryocytes Methylcellulose Microscopy Stem Cell Factor Stem Cells Thrombopoietin
To evaluate the number of circulating progenitor cells after mobilization, a colony-forming cell assay was employed. Red blood cells (RBCs) were lysed with BD Pharm Lyse buffer (BD Biosciences, San Jose, CA; http://www.bdbiosciences.com). Nucleated cells were subsequently washed twice and used for colony-formation assays. Briefly, cells were resuspended in methylcellulose base media provided by the manufacturer (R&D Systems, Inc., Minneapolis, MN; http://www.rndsystems.com) supplemented with granulocyte macrophage colony-stimulating factor (GM-CSF, 25 ng/ml) plus interleukin-3 (IL-3, 10 ng/ml) for colony forming units (CFU) granulocyte/macrophage (GM), granulocyte colony stimulating factor (G-CSF, 20 ng/ml) for CFU-granulocyte (G), macrophage colony stimulating factor (M-CSF, 10 ng/ml) for CFU macrophage (M), erythropoietin (EPO, 5 unit/ml, Stem cell Tech, http://www.stemcell.) plus stem cell factor (SCF, 5 ng/ml) for burst-forming units (BFU-E), and thrombopoietin (TPO, 100 ng/ml) for CFU-megakaryocytes (Megs). All growth factors were purchased from the same company unless otherwise mentioned. Cultures were incubated for 7 days, at which time they were scored under an inverted microscope for the number of each colonies.
Publication 2010
Biological Assay Buffers Cells Erythrocytes Erythropoietin Granulocyte Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte-Macrophage Colony Forming Units Granulocyte Colony-Stimulating Factor Growth Factor Macrophage Macrophage Colony-Stimulating Factor Megakaryocytes Methylcellulose Microscopy Stem Cell Factor Stem Cells Thrombopoietin

Most recents protocols related to «Thrombopoietin»

Example 5

Expansion and Differentiation of Haematopoietic Cells

The haematopoietic cells (e.g. haematopoietic stem cells) are stimulated using a supernatant growth factor suspension, to either develop more stem cells or differentiate into precursor cells (e.g. myeloid or granulocyte progenitor cells) or granulocytes. Suitable neutrophil synthesis methods are disclosed in Lieber et al, Blood, 2004 Feb. 1; 103(3):852-9, and Choi et al, Nat. Protoc., 2011 March; 6(3):296-313.

The protocol is composed of four major stages:

    • culturing and proliferation of haematopoietic cells;
    • short-term expansion of multipotent myeloid progenitors with a high dose of granulocyte-macrophage colony-stimulating factor (GM-CSF), a granulocyte colony-stimulating factor (G-CSF), a human growth hormone (HGH); serotonin, vitamin C, vitamin D, glutamine (Gln), arachidonic acid, AGE-albumin, interleukin-3 (IL-3), interleukin 8 (IL-8), Interleukin-4 (IL-4), Interleukin-6 (IL-6), interleukin-18 (IL-18), TNF-alpha, Flt-3 ligand, thrombopoietin, foetal bovine serum (FBS), or combinations thereof; and
    • directed differentiation of myeloid progenitors into neutrophils, eosinophils, dendritic cells (DCs), Langerhans cells (LCs), macrophages and osteoclasts.

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Patent 2024
Albumins Anabolism Arachidonic Acid Ascorbic Acid BLOOD Cell Proliferation Cells Dendritic Cells Eosinophil Ergocalciferol Fetal Bovine Serum flt3 ligand Glutamine Granulocyte Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte Colony-Stimulating Factor Granulocyte Progenitor Cells Growth Factor Hematopoietic System Interleukin-3 Interleukin-18 interleukin 18 protein, human Langerhans Cell Macrophage Malignant Neoplasms Neutrophil Osteoclasts Serotonin Stem Cells Stem Cells, Hematopoietic Thrombopoietin Tumor Necrosis Factor-alpha

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Publication 2023
Apheresis Body Weight Bone Marrow Cell Culture Techniques Cells Cloning Vectors Cytokine Dinoprostone flt3 ligand General Anesthesia Granulocyte Colony-Stimulating Factor Hematopoietic System Homo sapiens Interleukin-3 interleukin 20 Patients Pharmaceutical Adjuvants plerixafor Serum Stem Cell Factor Therapies, Biological Therapy, Gene Thrombopoietin Treatment Protocols

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Publication 2023
Cells Donors Ethics Committees, Research FER protein, human Ficoll Freezing Homo sapiens KITLG protein, human Ligands Microspheres Mus Stem Cells Stem Cells, Hematopoietic Sulfoxide, Dimethyl Thrombopoietin Umbilical Cord Blood
Sixty-one cytokines/chemokines in plasma were quantified using the U-plex biomarker NHP 61 plex (Meso Scale Diagnostics; MSD, MD, USA) to determine changes in CTACK (C-c motif chemokine ligand 27; CCL27), eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26), ENA-78, Fractalkine, FLT3L (FMS-like tyrosine kinase 3 ligand), G-CSF (granulocyte colony-stimulating factor), GM-CSF (granulocyte-macrophage colony-stimulating factor), I-309 (CCL1), GRO-α (CXCL1), IFN-α2a, IFN-γ, IL-1α (interleukin-1α), IL-1β, IL-1RA (interleukin-1 receptor antagonist), IL-2, IL-2Rα, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-9, IL-10, IL-12, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17F, IL-18, IL-22, IL-23, IP-10 (IFNγ inducible protein 10; CXCL10), I-TAC (Interferon-inducible T-cell alpha chemoattractant; CXCL11), MCP-1 (monocyte chemotactic protein-1; CCL2), MCP-2 (CCL8), MCP-3 (CCL7), MCP-4 (CCL13), M-CSF (macrophage colony-stimulating factor), MDC (macrophage-derived chemokine; CCL22), MIF (macrophage migration inhibition factor), MIP-1α (macrophage inflammatory protein 1α; CCL3), MIP-1β (CCL4), MIP-3α (CCL20), MIP-3β (CCL19), MIP-5 (CCL15), SDF-1α (stromal cell-derived factor-1 alpha; CXCL12), TARC (thymus and activation regulated chemokine), TNF-α (tumor necrosis factor-α), TNF-β, TPO (thrombopoietin), TRAIL (TNF-related apoptosis-inducing ligand), VEGF-α (vascular endothelial growth factor-α), and YKL-40 (chitinase-3-like protein 1) following the manufacturer instruction with minor modification [8 (link)]. U-plex plates were coated with respective biotinylated capture antibodies and incubated overnight on a shaker at 4 °C. Calibrator standards and diluted plasma samples were added to the individual wells after washing with wash buffer. The plate was incubated overnight on a shaker at 4 °C. The next day, the plate was washed with wash buffer and the detection antibody was added to the well and incubated on a shaker at room temperature for 1 h. The plate was finally washed and a read buffer was added. The plate was read immediately on an MSD microplate reader (MSD). The concentration of each cytokine and chemokine was determined based on the calibration standard curve and its respective signals.
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Publication 2023
Antibodies Biological Markers Buffers CCL1 protein, human CCL2 protein, human CCL3 protein, human CCL4 protein, human CCL7 protein, human CCL8 protein, human CCL15 protein, human CCL19 protein, human CCL20 protein, human CCL22 protein, human CCL26 protein, human CCL27 protein, human Chemokine Chemokine CCL22 Chemokine CCL24 Chemokine CXCL12 CHI3L1 protein, human Chitinases CXCL1 protein, human CXCL8 protein, human CXCL10 protein, human CXCL11 protein, human Cytokine Diagnosis Eotaxin-1 flt3 ligand Fractalkine Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte Colony-Stimulating Factor IL1A protein, human IL10 protein, human IL17F protein, human IL22 protein, human Immunoglobulins Interferon Type II Interleukin-1 beta Interleukin-12 Interleukin-13 Interleukin-15 Interleukin-17A Interleukin-17B Interleukin-27 Interleukin 1 Receptor Antagonist Protein Interleukin 17C interleukin 18 protein, human Ligands Macrophage Colony-Stimulating Factor Macrophage Migration Inhibitory Factor MCP-4 protein, human Monocyte Chemoattractant Protein-1 Plasma Small Inducible Cytokine A3 Stromal Cell-Derived Factor-1alpha Synapsin I Thrombopoietin Thymus Gland TNF protein, human TNF Related Apoptosis Inducing Ligand TNFSF10 protein, human Tumor Necrosis Factor-alpha TUMOR NECROSIS FACTOR BETA Vascular Endothelial Growth Factors
All BERK mice used for experiments were heterozygous for the sickle transgene (mouse βA human βS), confirmed by RP-HPLC with appropriate mouse and human globin controls. Male BERK mice were euthanized at 2–3 months of age by CO2 narcosis. Cells from bone marrow were harvested by flushing the tibiae, femora, and hip bones with PBS containing 0.5% BSA and 2 mM EDTA. Lineage cells from total bone marrow were isolated using the Lineage Cell Depletion Kit (Miltenyi Biotech, catalog number 130-090-858) according to the manufacturer’s protocol. Lineage cells were stained with APC-cKit (Thermo Fisher Scientific, catalog number 17-1171-83) and PE-Sca1 (BD Biosciences, catalog number 553336). LSK cells were sorted on an MoFlo Astrios Sorter (Beckman Coulter, Brea, CA, USA) and then cultured in SFEM medium (STEMCELL Technologies, catalog number 09600) containing 10% FBS (SAFC Biosciences) with 50 μM β-mercaptoethanol (Sigma-Aldrich, catalog number M3148), 20 ng/mL Flt3L, 20 ng/mL interleukin-6 (IL-6), 100 ng/mL stem cell factor (SCF), and 20 ng/mL thrombopoietin (TPO; PeproTech) for 24 h.
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Publication 2023
2-Mercaptoethanol Bone Marrow Cells CASP3 protein, human Cells Culture Media Edetic Acid Femur Globin Heterozygote High-Performance Liquid Chromatographies Hip Bone Homo sapiens Interleukin-6 Males Mus Narcosis Proto-Oncogene Protein c-kit Stem Cell Factor Stem Cells Thrombopoietin Tibia Transgenes

Top products related to «Thrombopoietin»

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Thrombopoietin is a glycoprotein that regulates the production and differentiation of platelets in the bone marrow. It is a key regulator of megakaryocyte and platelet production.
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The SCF is a versatile laboratory instrument designed to perform supercritical fluid extraction and chromatography. It utilizes the unique properties of supercritical fluids, such as adjustable solvent power and low viscosity, to efficiently extract, fractionate, and purify a wide range of compounds. The core function of the SCF is to provide researchers and analysts with a powerful tool for sample preparation, purification, and analysis across various industries and applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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IL-3 is a recombinant protein that supports the growth and differentiation of hematopoietic stem and progenitor cells. It plays a critical role in the regulation of blood cell production.
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IL-6 is a lab equipment product that measures the concentration of interleukin-6 (IL-6), a cytokine involved in various biological processes. The core function of this product is to quantify IL-6 levels in samples.
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Thrombopoietin (TPO) is a glycoprotein hormone that plays a crucial role in the regulation of platelet production. It is responsible for stimulating the proliferation and differentiation of megakaryocytes, which are the precursor cells that give rise to platelets. TPO is an essential component in the maintenance of normal platelet levels and the management of various hematological conditions.
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The FLT3 ligand is a laboratory reagent used for cell culture and related experiments. It functions as a growth factor that binds to the FLT3 receptor, stimulating the proliferation and differentiation of hematopoietic stem cells.
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
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Thrombopoietin is a glycoprotein that regulates the production and differentiation of megakaryocytes, the precursor cells of blood platelets. It plays a crucial role in the regulation of platelet production.
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IMDM (Iscove's Modified Dulbecco's Medium) is a cell culture medium formulated for the growth and maintenance of a wide variety of cell types, including hematopoietic cells. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.

More about "Thrombopoietin"

Thrombopoietin (TPO) is a critical glycoprotein hormone that regulates the production and function of platelets, the small blood cells responsible for clotting.
Primarily synthesized in the liver and kidneys, TPO acts on megakaryocytes, the precursor cells that give rise to platelets, stimulating their proliferation and differentiation.
This crucial role in maintaining platelet homeostasis makes TPO an important therapeutic target for various platelet disorders and thrombotic conditions.
Researchers can leverage the innovative AI platform of PubCompare.ai to optimize reproducible protocols and identify the best products for studying TPO and its multifaceted functions in health and disease.
The platform's advanced AI-driven comparisons enable users to confidently navigate the wealth of literature, pre-prints, and patents, ensuring they can replicate their research and achieve optimal results.
Beyond TPO, the PubCompare.ai tool can also assist in the study of other critical hematopoietic factors, such as stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), and FLT3 ligand, which play pivotal roles in the development and maintenance of various blood cell types.
Additionally, the platform can help researchers optimize the use of common cell culture supplements like penicillin/streptomycin and L-glutamine, which are essential for maintaining cell viability and ensuring the reproducibility of experiments.
Experience the future of scientific reproducibility with PubCompare.ai and unlock the full potential of your research on Thrombopoietin and related hematopoietic factors.