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Thrombopoietin
Thrombopoietin
Thrombopoietin is a glycoprotein hormone that regualtes the production and function of platelets.
It is produced primarily by the liver and kidneys, and acts on megakaryocytes to stimulate their proliferation and differentiation.
Thrombopoietin plays a crucial role in maintaining platelet homeostasis and is an important target for therapeutic interventions related to platelet disorders and thrombotic conditions.
Researchers can leverage PubCompare.ai's innovative AI platform to optimize reproducible protocols and identify the best products for studying thrombopoietin and its role in health and disease.
It is produced primarily by the liver and kidneys, and acts on megakaryocytes to stimulate their proliferation and differentiation.
Thrombopoietin plays a crucial role in maintaining platelet homeostasis and is an important target for therapeutic interventions related to platelet disorders and thrombotic conditions.
Researchers can leverage PubCompare.ai's innovative AI platform to optimize reproducible protocols and identify the best products for studying thrombopoietin and its role in health and disease.
Most cited protocols related to «Thrombopoietin»
QQ culture medium of Stem Line II (Sigma‐Aldrich, St. Louis, MO) contained the 5 human recombinant proteins: stem cell factor (SCF); thrombopoietin (TPO); Flt‐3 ligand; vascular endothelial growth factor (VEGF); and interleukin (IL)‐6. Then, isolated PBMNCs were cultured for 7 days at the cell density of 2×106 cells/2 mL QQ culture medium per well of 6‐well Primaria tissue culture plate (BD Falcon; BD Biosiences, San Jose, CA). Cell density in QQ culture was corresponded to the approximate density of 1×106 MNCs in 1 mL of PB. Culture well plates and the contents of QQ culture medium are listed in Tables 1 and 2 .
Cell Culture Techniques
Cells
Culture Media
flt3 ligand
Interleukin-6
NR4A2 protein, human
Stem, Plant
Stem Cell Factor
Thrombopoietin
Tissues
Vascular Endothelial Growth Factors
Animals
Cells
Cloning Vectors
flt3 ligand
Granulocyte Colony-Stimulating Factor
Homo sapiens
Infection
Macaca
retronectin
Serum Albumin, Human
Stem Cell Factor
Sulfate, Protamine
Thrombopoietin
Transplantation
2-Mercaptoethanol
Amino Acids
Biological Assay
Cells
Cloning Vectors
Ethics Committees, Research
Flow Cytometry
flt3 ligand
Glutamine
Human Embryonic Stem Cells
Hyperostosis, Diffuse Idiopathic Skeletal
KLF4 protein, human
Malignant Neoplasms
Methylation
Oncogenes, myc
Patients
PBMC Peripheral Blood Mononuclear Cells
POU5F1 protein, human
retronectin
SOX2 protein, human
Stem Cell Factor
Teratoma
Thrombopoietin
Valproic Acid
To evaluate the number of circulating progenitor cells after mobilization, a colony-forming cell assay was employed. Red blood cells (RBCs) were lysed with BD Pharm Lyse buffer (BD Biosciences, San Jose, CA; http://www.bdbiosciences.com ). Nucleated cells were subsequently washed twice and used for colony-formation assays. Briefly, cells were resuspended in methylcellulose base media provided by the manufacturer (R&D Systems, Inc., Minneapolis, MN; http://www.rndsystems.com ) supplemented with granulocyte macrophage colony-stimulating factor (GM-CSF, 25 ng/ml) plus interleukin-3 (IL-3, 10 ng/ml) for colony forming units (CFU) granulocyte/macrophage (GM), granulocyte colony stimulating factor (G-CSF, 20 ng/ml) for CFU-granulocyte (G), macrophage colony stimulating factor (M-CSF, 10 ng/ml) for CFU macrophage (M), erythropoietin (EPO, 5 unit/ml, Stem cell Tech, http://www.stemcell .) plus stem cell factor (SCF, 5 ng/ml) for burst-forming units (BFU-E), and thrombopoietin (TPO, 100 ng/ml) for CFU-megakaryocytes (Megs). All growth factors were purchased from the same company unless otherwise mentioned. Cultures were incubated for 7 days, at which time they were scored under an inverted microscope for the number of each colonies.
Biological Assay
Buffers
Cells
Erythrocytes
Erythropoietin
Granulocyte
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony Forming Units
Granulocyte Colony-Stimulating Factor
Growth Factor
Macrophage
Macrophage Colony-Stimulating Factor
Megakaryocytes
Methylcellulose
Microscopy
Stem Cell Factor
Stem Cells
Thrombopoietin
Biological Assay
Buffers
Cells
Erythrocytes
Erythropoietin
Granulocyte
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony Forming Units
Granulocyte Colony-Stimulating Factor
Growth Factor
Macrophage
Macrophage Colony-Stimulating Factor
Megakaryocytes
Methylcellulose
Microscopy
Stem Cell Factor
Stem Cells
Thrombopoietin
Most recents protocols related to «Thrombopoietin»
Example 5
Expansion and Differentiation of Haematopoietic Cells
The haematopoietic cells (e.g. haematopoietic stem cells) are stimulated using a supernatant growth factor suspension, to either develop more stem cells or differentiate into precursor cells (e.g. myeloid or granulocyte progenitor cells) or granulocytes. Suitable neutrophil synthesis methods are disclosed in Lieber et al, Blood, 2004 Feb. 1; 103(3):852-9, and Choi et al, Nat. Protoc., 2011 March; 6(3):296-313.
The protocol is composed of four major stages:
-
- culturing and proliferation of haematopoietic cells;
- short-term expansion of multipotent myeloid progenitors with a high dose of granulocyte-macrophage colony-stimulating factor (GM-CSF), a granulocyte colony-stimulating factor (G-CSF), a human growth hormone (HGH); serotonin, vitamin C, vitamin D, glutamine (Gln), arachidonic acid, AGE-albumin, interleukin-3 (IL-3), interleukin 8 (IL-8), Interleukin-4 (IL-4), Interleukin-6 (IL-6), interleukin-18 (IL-18), TNF-alpha, Flt-3 ligand, thrombopoietin, foetal bovine serum (FBS), or combinations thereof; and
- directed differentiation of myeloid progenitors into neutrophils, eosinophils, dendritic cells (DCs), Langerhans cells (LCs), macrophages and osteoclasts.
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Albumins
Anabolism
Arachidonic Acid
Ascorbic Acid
BLOOD
Cell Proliferation
Cells
Dendritic Cells
Eosinophil
Ergocalciferol
Fetal Bovine Serum
flt3 ligand
Glutamine
Granulocyte
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte Colony-Stimulating Factor
Granulocyte Progenitor Cells
Growth Factor
Hematopoietic System
Interleukin-3
Interleukin-18
interleukin 18 protein, human
Langerhans Cell
Macrophage
Malignant Neoplasms
Neutrophil
Osteoclasts
Serotonin
Stem Cells
Stem Cells, Hematopoietic
Thrombopoietin
Tumor Necrosis Factor-alpha
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Apheresis
Body Weight
Bone Marrow
Cell Culture Techniques
Cells
Cloning Vectors
Cytokine
Dinoprostone
flt3 ligand
General Anesthesia
Granulocyte Colony-Stimulating Factor
Hematopoietic System
Homo sapiens
Interleukin-3
interleukin 20
Patients
Pharmaceutical Adjuvants
plerixafor
Serum
Stem Cell Factor
Therapies, Biological
Therapy, Gene
Thrombopoietin
Treatment Protocols
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Cells
Donors
Ethics Committees, Research
FER protein, human
Ficoll
Freezing
Homo sapiens
KITLG protein, human
Ligands
Microspheres
Mus
Stem Cells
Stem Cells, Hematopoietic
Sulfoxide, Dimethyl
Thrombopoietin
Umbilical Cord Blood
Sixty-one cytokines/chemokines in plasma were quantified using the U-plex biomarker NHP 61 plex (Meso Scale Diagnostics; MSD, MD, USA) to determine changes in CTACK (C-c motif chemokine ligand 27; CCL27), eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26), ENA-78, Fractalkine, FLT3L (FMS-like tyrosine kinase 3 ligand), G-CSF (granulocyte colony-stimulating factor), GM-CSF (granulocyte-macrophage colony-stimulating factor), I-309 (CCL1), GRO-α (CXCL1), IFN-α2a, IFN-γ, IL-1α (interleukin-1α), IL-1β, IL-1RA (interleukin-1 receptor antagonist), IL-2, IL-2Rα, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-9, IL-10, IL-12, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17F, IL-18, IL-22, IL-23, IP-10 (IFNγ inducible protein 10; CXCL10), I-TAC (Interferon-inducible T-cell alpha chemoattractant; CXCL11), MCP-1 (monocyte chemotactic protein-1; CCL2), MCP-2 (CCL8), MCP-3 (CCL7), MCP-4 (CCL13), M-CSF (macrophage colony-stimulating factor), MDC (macrophage-derived chemokine; CCL22), MIF (macrophage migration inhibition factor), MIP-1α (macrophage inflammatory protein 1α; CCL3), MIP-1β (CCL4), MIP-3α (CCL20), MIP-3β (CCL19), MIP-5 (CCL15), SDF-1α (stromal cell-derived factor-1 alpha; CXCL12), TARC (thymus and activation regulated chemokine), TNF-α (tumor necrosis factor-α), TNF-β, TPO (thrombopoietin), TRAIL (TNF-related apoptosis-inducing ligand), VEGF-α (vascular endothelial growth factor-α), and YKL-40 (chitinase-3-like protein 1) following the manufacturer instruction with minor modification [8 (link)]. U-plex plates were coated with respective biotinylated capture antibodies and incubated overnight on a shaker at 4 °C. Calibrator standards and diluted plasma samples were added to the individual wells after washing with wash buffer. The plate was incubated overnight on a shaker at 4 °C. The next day, the plate was washed with wash buffer and the detection antibody was added to the well and incubated on a shaker at room temperature for 1 h. The plate was finally washed and a read buffer was added. The plate was read immediately on an MSD microplate reader (MSD). The concentration of each cytokine and chemokine was determined based on the calibration standard curve and its respective signals.
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Antibodies
Biological Markers
Buffers
CCL1 protein, human
CCL2 protein, human
CCL3 protein, human
CCL4 protein, human
CCL7 protein, human
CCL8 protein, human
CCL15 protein, human
CCL19 protein, human
CCL20 protein, human
CCL22 protein, human
CCL26 protein, human
CCL27 protein, human
Chemokine
Chemokine CCL22
Chemokine CCL24
Chemokine CXCL12
CHI3L1 protein, human
Chitinases
CXCL1 protein, human
CXCL8 protein, human
CXCL10 protein, human
CXCL11 protein, human
Cytokine
Diagnosis
Eotaxin-1
flt3 ligand
Fractalkine
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte Colony-Stimulating Factor
IL1A protein, human
IL10 protein, human
IL17F protein, human
IL22 protein, human
Immunoglobulins
Interferon Type II
Interleukin-1 beta
Interleukin-12
Interleukin-13
Interleukin-15
Interleukin-17A
Interleukin-17B
Interleukin-27
Interleukin 1 Receptor Antagonist Protein
Interleukin 17C
interleukin 18 protein, human
Ligands
Macrophage Colony-Stimulating Factor
Macrophage Migration Inhibitory Factor
MCP-4 protein, human
Monocyte Chemoattractant Protein-1
Plasma
Small Inducible Cytokine A3
Stromal Cell-Derived Factor-1alpha
Synapsin I
Thrombopoietin
Thymus Gland
TNF protein, human
TNF Related Apoptosis Inducing Ligand
TNFSF10 protein, human
Tumor Necrosis Factor-alpha
TUMOR NECROSIS FACTOR BETA
Vascular Endothelial Growth Factors
All BERK mice used for experiments were heterozygous for the sickle transgene (mouse βA human βS), confirmed by RP-HPLC with appropriate mouse and human globin controls. Male BERK mice were euthanized at 2–3 months of age by CO2 narcosis. Cells from bone marrow were harvested by flushing the tibiae, femora, and hip bones with PBS containing 0.5% BSA and 2 mM EDTA. Lineage− cells from total bone marrow were isolated using the Lineage Cell Depletion Kit (Miltenyi Biotech, catalog number 130-090-858) according to the manufacturer’s protocol. Lineage− cells were stained with APC-cKit (Thermo Fisher Scientific, catalog number 17-1171-83) and PE-Sca1 (BD Biosciences, catalog number 553336). LSK cells were sorted on an MoFlo Astrios Sorter (Beckman Coulter, Brea, CA, USA) and then cultured in SFEM medium (STEMCELL Technologies, catalog number 09600) containing 10% FBS (SAFC Biosciences) with 50 μM β-mercaptoethanol (Sigma-Aldrich, catalog number M3148), 20 ng/mL Flt3L, 20 ng/mL interleukin-6 (IL-6), 100 ng/mL stem cell factor (SCF), and 20 ng/mL thrombopoietin (TPO; PeproTech) for 24 h.
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2-Mercaptoethanol
Bone Marrow Cells
CASP3 protein, human
Cells
Culture Media
Edetic Acid
Femur
Globin
Heterozygote
High-Performance Liquid Chromatographies
Hip Bone
Homo sapiens
Interleukin-6
Males
Mus
Narcosis
Proto-Oncogene Protein c-kit
Stem Cell Factor
Stem Cells
Thrombopoietin
Tibia
Transgenes
Top products related to «Thrombopoietin»
Sourced in United States, Canada, United Kingdom
Thrombopoietin is a glycoprotein that regulates the production and differentiation of platelets in the bone marrow. It is a key regulator of megakaryocyte and platelet production.
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The SCF is a versatile laboratory instrument designed to perform supercritical fluid extraction and chromatography. It utilizes the unique properties of supercritical fluids, such as adjustable solvent power and low viscosity, to efficiently extract, fractionate, and purify a wide range of compounds. The core function of the SCF is to provide researchers and analysts with a powerful tool for sample preparation, purification, and analysis across various industries and applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
Sourced in United States, United Kingdom, Germany, Canada, Israel, Switzerland, France, Macao, Japan
IL-3 is a recombinant protein that supports the growth and differentiation of hematopoietic stem and progenitor cells. It plays a critical role in the regulation of blood cell production.
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IL-6 is a lab equipment product that measures the concentration of interleukin-6 (IL-6), a cytokine involved in various biological processes. The core function of this product is to quantify IL-6 levels in samples.
Sourced in United States, Canada
Thrombopoietin (TPO) is a glycoprotein hormone that plays a crucial role in the regulation of platelet production. It is responsible for stimulating the proliferation and differentiation of megakaryocytes, which are the precursor cells that give rise to platelets. TPO is an essential component in the maintenance of normal platelet levels and the management of various hematological conditions.
Sourced in United States, Canada, United Kingdom, Germany
The FLT3 ligand is a laboratory reagent used for cell culture and related experiments. It functions as a growth factor that binds to the FLT3 receptor, stimulating the proliferation and differentiation of hematopoietic stem cells.
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
Sourced in United States
Thrombopoietin is a glycoprotein that regulates the production and differentiation of megakaryocytes, the precursor cells of blood platelets. It plays a crucial role in the regulation of platelet production.
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IMDM (Iscove's Modified Dulbecco's Medium) is a cell culture medium formulated for the growth and maintenance of a wide variety of cell types, including hematopoietic cells. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.
More about "Thrombopoietin"
Thrombopoietin (TPO) is a critical glycoprotein hormone that regulates the production and function of platelets, the small blood cells responsible for clotting.
Primarily synthesized in the liver and kidneys, TPO acts on megakaryocytes, the precursor cells that give rise to platelets, stimulating their proliferation and differentiation.
This crucial role in maintaining platelet homeostasis makes TPO an important therapeutic target for various platelet disorders and thrombotic conditions.
Researchers can leverage the innovative AI platform of PubCompare.ai to optimize reproducible protocols and identify the best products for studying TPO and its multifaceted functions in health and disease.
The platform's advanced AI-driven comparisons enable users to confidently navigate the wealth of literature, pre-prints, and patents, ensuring they can replicate their research and achieve optimal results.
Beyond TPO, the PubCompare.ai tool can also assist in the study of other critical hematopoietic factors, such as stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), and FLT3 ligand, which play pivotal roles in the development and maintenance of various blood cell types.
Additionally, the platform can help researchers optimize the use of common cell culture supplements like penicillin/streptomycin and L-glutamine, which are essential for maintaining cell viability and ensuring the reproducibility of experiments.
Experience the future of scientific reproducibility with PubCompare.ai and unlock the full potential of your research on Thrombopoietin and related hematopoietic factors.
Primarily synthesized in the liver and kidneys, TPO acts on megakaryocytes, the precursor cells that give rise to platelets, stimulating their proliferation and differentiation.
This crucial role in maintaining platelet homeostasis makes TPO an important therapeutic target for various platelet disorders and thrombotic conditions.
Researchers can leverage the innovative AI platform of PubCompare.ai to optimize reproducible protocols and identify the best products for studying TPO and its multifaceted functions in health and disease.
The platform's advanced AI-driven comparisons enable users to confidently navigate the wealth of literature, pre-prints, and patents, ensuring they can replicate their research and achieve optimal results.
Beyond TPO, the PubCompare.ai tool can also assist in the study of other critical hematopoietic factors, such as stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), and FLT3 ligand, which play pivotal roles in the development and maintenance of various blood cell types.
Additionally, the platform can help researchers optimize the use of common cell culture supplements like penicillin/streptomycin and L-glutamine, which are essential for maintaining cell viability and ensuring the reproducibility of experiments.
Experience the future of scientific reproducibility with PubCompare.ai and unlock the full potential of your research on Thrombopoietin and related hematopoietic factors.