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Trypticase

Trypticase, also known as Tryptic Soy Broth, is a widely used culture medium in microbiology and biotechnology.
It provides a nutrient-rich environment for the growth of a variety of microorganisms, including bacteria, yeasts, and fungi.
Trypticase contains a complex mixture of peptones, which are derived from the enzymatic digestion of casein, as well as additional nutrients such as dextrose and salts.
This medium supports the replication and maintenance of microbial cells, making it a crucial component in various experimental and industrial applications.
Researchers can easily locate and compare the best Trypticase protocols from literature, preprints, and patents using the intelligent comparison tools of PubComapre.ai, an AI-driven platform that enhances research reproducibility and accuracy.
By optimizing their experiments with Trypticase, scientists can drive their research forward and advance their understanding of microbial processes and systems.

Most cited protocols related to «Trypticase»

1
Bacterial Strain and Plasmid Cultivation
Cited 22 times
Bacterial strains, plasmids, and oligonucleotides used in this study are described in Table 1. E. coli, S. aureus, and S. epidermidis were routinely cultured at 37°C in L broth (1% tryptone, 0.5% yeast extract, 0.5% NaCl), Trypticase soy broth (TSB) (Difco) or brain heart infusion broth (BHI) (Difco). For growth on agar, L broth or brain heart infusion broth was solidified with 1.5% agar, yielding LBA and BHIA, respectively. The following antibiotics and concentrations were used: chloramphenicol (Cm), 10 µg/ml; kanamycin, 50 µg/ml; erythromycin (Em), 25 µg/ml; and carbenicillin, 100 µg/ml (Sigma).
Oligonucleotides and DNA sequencing were purchased from IDT. Restriction enzymes and LigaFAST T4 DNA ligase were purchased from NEB and Promega, respectively. High-fidelity PCR was performed with KOD Hotstart DNA polymerase (Novagen) or Phusion DNA polymerase (Finnzymes) on genomic DNA isolated with the Genelute bacterial genomic DNA kit (Sigma). Plasmids and PCR products were purified using WizardPlus kits (Promega). To isolate plasmid DNA from S. aureus, a 10-ml overnight culture was treated with 100 µg lysostaphin (Ambi Products, New York) in P1 buffer for 30 min at room temperature and then processed as recommended by the manufacturer (GeneJET plamsid miniprep kit; Fermentas).
For colony PCR, a small amount of colonial growth was touched to the side of a PCR tube and microwaved for 5 min at 800 W. The tube was placed on ice, Phire Hotstart II master mix (Finnzymes) was added to the PCR tube, and thermocycling conditions were conducted as recommended by the manufacturer.
Monk I.R., Shah I.M., Xu M., Tan M.W, & Foster T.J. (2012). Transforming the Untransformable: Application of Direct Transformation To Manipulate Genetically Staphylococcus aureus and Staphylococcus epidermidis. mBio, 3(2), e00277-11.
Publication 2012
Agar Antibiotics Bacteria Brain Buffers Carbenicillin Chloramphenicol DNA, Bacterial DNA-Directed DNA Polymerase DNA Restriction Enzymes Erythromycin Escherichia coli Genome Heart Kanamycin Lysostaphin Oligonucleotides Plasmids Promega Sodium Chloride Staphylococcus aureus Staphylococcus epidermidis Strains T4 DNA Ligase trypticase-soy broth Yeast, Dried
2
Screening for Quorum Sensing Inhibitors in Pseudomonas aeruginosa
Cited 21 times

P. aeruginosa PAO1 was propagated on trypticase soy agar (TSA) for plate-based assays or in trypticase soy broth (TSB) for liquid culture. M9 growth media supplemented with 0.4% (w/v) glucose and 0.4% (wt/v) casamino acids was used for biofilm formation experiments. Culture media (TSB, TSA, M9 salts and casamino acids) were obtained from Difco/Becton Dickinson (Franklin Lakes, NJ, USA) and all other reagents (phosphate buffered saline, glucose, ethanol and crystal violet) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Corning 35–1172 flat-bottomed polystyrene 96-well plates were used for biofilm formation experiments and optical density measurements were performed in a Tecan M-200 (Durham, NC, USA) plate reader. Optical micrographs of biofilms were obtained using a Nikon Eclipse 80i microscope.
A microplate based assay, modified from Junker et al.[32] (link) was used to screen compounds for QSI. Briefly, P. aeruginosa PAO1 was grown in TSB for 18 h at 37°C with rotary shaking at 225 rpm. The culture was then centrifuged at 14,000 rpm and rinsed with phosphate buffered saline (PBS, pH 7.4) three times, then was re-suspended in M9 minimal growth media to approximately 1×107 cfu/ml (determined by OD and plate count assay). Test compounds were dissolved in DMSO and were added to sterile distilled water to achieve concentrations ranging from 0.1–10 mM while keeping DMSO at a maximum of 1% (v/v). P. aeruginosa inocula (360 µl) were then pre-mixed with 40 µl of the test compound solutions to achieve final compound concentrations ranging from 0.01–1 mM. An aliquot (100 µl) of this cell/compound mixture was then added to three separate wells in a 96-well microplate for replicate testing. For control wells (no inhibitor), dilute DMSO was added to the inocula instead of test compounds, to a final concentration of 1% (v/v). Optical density (OD600nm) measurements were performed immediately after inoculation and after 24 h incubation at 37°C (without shaking) to monitor planktonic cell growth. To determine the amount of biofilm formation, supernatant from the microplate wells was gently removed and the wells were washed twice with 150 µl of PBS using a multichannel pipette. The remaining biofilm was then stained using 100 µl of a 0.2% (w/v) crystal violet solution for 15 min at room temperature. The crystal violet was then removed from the wells, the wells were rinsed four times with PBS, and then 100 µl of 95% ethanol was added to extract the crystal violet solution from the biofilm. The OD600nm of the extracted crystal violet was then measured, yielding a measure of biofilm formation (relative to the control). For optical imaging, crystal violet stained biofilms were washed with distilled water and no ethanol extraction was performed.
In addition to crystal violet based quantification of biofilm biomass, cell viability within biofilms exposed to inhibitor compounds was determined using the formazan dye-based MTT assay (Cell Proliferation Kit I, Roche Diagnostics, Mannheim, Germany). This assay has previously been described for determination of biofilm cell viability [43] (link)–[45] (link). Briefly, biofilms were grown in 96 well microplates for 24 h as described above, in the presence and absence of inhibitor compounds. After this initial inoculation period, planktonic cells were removed and the remaining biofilm was gently rinsed three times with 100 µl of PBS. After rinsing, 100 µl of PBS and 10 µl of the MTT labeling reagent were added and the suspension was incubated for 4 h at 37°C, followed by addition of 100 µl of solubilization solution. Plates were then incubated for 24 h at 37°C and absorbance measurements were taken using a Tecan M-200 plate reader at 560 nm (peak absorbance for the formazan dye breakdown product) and at 700 nm (reference wavelength for the intact dye).
Cady N.C., McKean K.A., Behnke J., Kubec R., Mosier A.P., Kasper S.H., Burz D.S, & Musah R.A. (2012). Inhibition of Biofilm Formation, Quorum Sensing and Infection in Pseudomonas aeruginosa by Natural Products-Inspired Organosulfur Compounds. PLoS ONE, 7(6), e38492.
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Publication 2012
Agar Biofilms Biological Assay casamino acids Catabolism Cell Proliferation Cells Cell Survival Culture Media Diagnosis DNA Replication Ethanol Formazans Glucose M-200 Microscopy Phosphates Plankton Polystyrenes Pseudomonas aeruginosa Saline Solution Salts Sterility, Reproductive Sulfoxide, Dimethyl Technique, Dilution trypticase-soy broth Vaccination Violet, Gentian Vision
3
Optimizing Meningococcal sodC Assay
Cited 13 times
Control isolates used for assay design and optimization are defined in Table
S1.
626 cell lysates were used to determine the sensitivity of the
sodC assay (Table 1 and Table S2), including lysates prepared from a
temporally and geographically dispersed convenience sample of isolates from the
CDC Meningitis Laboratory strain collection (received 1993–2008,
n = 106) and all isolates from a US carriage study
(n = 520) [20] , [21] (link) known to be Nm by SASG [1] , [2] , rt-PCR serogrouping [5] (link), NH
strips (bioMérieux® sa), and Cystine Trypticase Agar (CTA) sugars
(Remel) [1] ,
[2] . To
further confirm identification, multilocus sequence typing (MLST) was performed
on all U.S. carriage study and ctrA-negative NG isolates.
The specificity of the sodC assay for detecting only
meningococci was determined using cell lysates from a total of 244 non-Nm
isolates (Table 2).
Dolan Thomas J., Hatcher C.P., Satterfield D.A., Theodore M.J., Bach M.C., Linscott K.B., Zhao X., Wang X., Mair R., Schmink S., Arnold K.E., Stephens D.S., Harrison L.H., Hollick R.A., Andrade A.L., Lamaro-Cardoso J., de Lemos A.P., Gritzfeld J., Gordon S., Soysal A., Bakir M., Sharma D., Jain S., Satola S.W., Messonnier N.E, & Mayer L.W. (2011). sodC-Based Real-Time PCR for Detection of Neisseria meningitidis. PLoS ONE, 6(5), e19361.
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Publication 2011
Agar Biological Assay Cells Cystine Hypersensitivity Meningitis Reverse Transcriptase Polymerase Chain Reaction Strains Sugars trypticase
4
Isolation and Identification of Lactic Acid Bacteria from Korean Fermented Soybean Paste
Cited 10 times
A total of 35 indigenous LAB (Lactic acid bacteria) strains (Table 1) isolated from the Korean fermented soybean paste, were provided by Soonchang Jang Ryu Saupso Company-Korea. Based on different colony morphology using MRS (de Man, Rogosa and Sharpe) selective media and further identified on the bases of 16S rRNA sequencing using universal primers (27F-5ʹ AGA GTT TGA TCM TGG CTC AG 3ʹ and 1492R-5ʹ GGT TAC CTT GTT ACG ACT 3ʹ). The LAB, Pseudomonas aeruginosa PA14 and Escherichia coli OP50 bacterial strains were stored at – 80°C in De Man, Rogosa & Sharpe (MRS, BD Biosciences, San Jose, CA, USA) medium. Trypticase soy broth (TSB, BD Biosciences, San Jose, CA, USA) was used to cultivate Pseudomonas aeruginosa PA14 and Escherichia coli OP50, which were obtained from the US Food Fermentation Laboratory Culture Collection (Wyndmoor, PA., USA) supplemented with 30% glycerol. Each culture was streaked from frozen stocks onto TSA and MRS (BD Biosciences) and incubated at 37°C for 24 h.10.1080/21505594.2018.1518088-T0001

Bacterial strains used in this study All 35 LAB (lactic acid bacterial) strains were isolated from Korean fermented soybean paste.

SN.oAccession NoIdentification NumberBacterial Strains
01637,912ATCC25922E.coliOP50
02208,963UCBPP-PA14Pseudomonas aeruginosa P14
L01SRCM100425MAD-13Enterococcus faecalis
L02SRCM100479SC54Enterococcus faecium
L03SRCM100426CK-5Enterococcus faecium
L04SRCM100328SCL1421Enterococcus lactis
L05SRCM100318SDL1411Lactobacillus brevis
L06SRCM100315SDL1408Lactobacillus brevis
L07SRCM100991JBNU38Lactobacillus curvatus
L08SRCM100476SC48Lactobacillus pentosus
L09SRCM100436JDFM44Lactobacillus plantatrum
L10SRCM100435JDFM33Lactobacillus rhamnosus
L11SRCM100434JDFM6Lactobacillus rhamnosus
L12SRCM100478SC53Leuconstoc citreum
L13SRCM100744JBNU10Leuconstoc mesenteroides
L14SRCM100474SC46Leuconstoc paramesenteroides
L15SRCM100327SCL1420Pediococcus acidilatic
L16SRCM100325SKL1418Pediococcus acidilatic
L17SRCM100321SDL1414Pediococcus acidilatic
L18SRCM100313SDL1406Pediococcus acidilatic
L19SRCM100312SDL1405Pediococcus acidilatic
L20SRCM100309SDL1402Pediococcus acidilatic
L21SRCM100427DM9Pediococcus acidilatic
L22SRCM100424MAC11Pediococcus pentosaceus
L23SRCM100323SDL1416Pediococcus pentosaceus
L24SRCM100322SDL1415Pediococcus pentosaceus
L25SRCM100308SDL1401Pediococcus pentosaceus
L26SRCM100879SCML337Streptococcus thermophilus
L27SRCM100872SCML300Streptococcus thermophilus
L28SRCM100182SCCB2306Weissella cibaria
L29SRCM100196SCSB2320Weissella confusa
L30SRCM100194SCKB2318Weissella confusa
L31SRCM100966JBNU2Weissella koreensis
L32SRCM100183SCCB2307Weissella cibaria
L33SRCM100316SDL1409Pediococcus pentosaceus
L34SRCM100320SDL1413Lactobacillus plantatrum
L35SRCM100467SC25Lactobacillus arizonensis
Chelliah R., Choi J.G., Hwang S.B., Park B.J., Daliri E.B., Kim S.H., Wei S., Ramakrishnan S.R, & Oh D.H. (2018). In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model. Virulence, 9(1), 1489-1507.
Publication 2018
Bacteria Escherichia coli Fermentation Food Freezing Glycerin Koreans Lactic Acid Lactobacillales Oligonucleotide Primers Paste PRO 140 Pseudomonas aeruginosa RNA, Ribosomal, 16S Soybeans Strains trypticase-soy broth
5
Murine Models of Staphylococcus aureus Pathogenesis
Cited 9 times
USA300 wild-type and isogenic lukS/F-PV mutant S. aureus strains (LAC and LACΔpvl, respectively) were described previously [11 (link)].
Female BALB/cJ (The Jackson Laboratory) and BALB/cAnNHsd (Harlan) mice (7–9 weeks) were housed in microisolator cages and received food and water ad libitum. All studies conformed to guidelines set forth by the National Institutes of Health and were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID).
For each experiment, overnight cultures of S. aureus grown in trypticase soy broth (TSB) were diluted 1:200 and grown at 37°C with shaking (250 rpm) to mid-exponential phase of growth (OD600 = 0.75). Staphylococci were washed in Dulbecco’s PBS (DPBS) and resuspended in DPBS at 2×108 colony forming units (CFUs)/mL. Two independent cultures were used to generate inocula for subsequent infection (e.g., 2 duplicate cultures×10 mice each = 20 mice total per S. aureus strain). Mice were anesthetized with isoflurane and inoculated by subcutaneous injection in the shaved right flank with 50 kL of DPBS containing 1×107 S. aureus, an inoculum determined in preliminary studies to produce consistent dermonecrotic abscesses within 2 days. Mice were weighed before inoculation and at 24-hour intervals for 14 days. Since abscesses were relatively flat, the area of each was calculated daily using the formula A = π(l × w)/2 as described [11 (link)].
To determine CFUs at the site of infection, a second set of mice (14–15 each of BALB/cJ and BALB/cAnNHsd) were inoculated as described above. Four mice were injected with DPBS. On day 4 post-infection, mice were euthanized and the abscessed skin and surrounding tissue were removed. Tissue samples were weighed, homogenized in DPBS, diluted in saline and plated on trypticase soy agar. CFUs were enumerated the following day. No bacteria were recovered from mice injected with DPBS.
For murine lung infections examining weight loss, lung CFU recovery and histopathology, LAC and LACΔpvl were prepared as described by Labandeira-Rey, et al. [12 (link)]. Briefly, overnight cultures grown in TSB were refreshed 1:100 in media and grown with shaking to an OD600 of 1.0. Bacteria were sedimented by centrifugation, washed in PBS, and suspended at a concentration of 4–6×107 CFUs per 20-μl volume of PBS for intranasal inoculation. For acute lethal disease studies, S. aureus strains were grown at 37°C in TSB to an OD660 of 0.5. Culture aliquots (50 ml) were centrifuged, washed in PBS, and suspended in 750 μl PBS (3–4×108 CFUs per 30 μl volume).
Animal experiments were reviewed, approved and supervised by the IACUC at the University of Chicago. For lung infection, 7 week old mice (BALB/cJ or BALB/cAnNHsd) were anesthetized prior to inoculation of the S. aureus suspension into the left nare as previously described [14 (link)]. Microbiologic and pathologic correlates of disease were assessed 48 hours post-infection, also as previously described [14 (link)].
Wardenburg J.B., Palazzolo-Ballance A.M., Otto M., Schneewind O, & DeLeo F.R. (2008). Panton-Valentine leukocidin is not a virulence determinant in murine models of community-associated methicillin-resistant Staphylococcus aureus disease. The Journal of infectious diseases, 198(8), 1166-1170.
Publication 2008
Acute Disease Agar Bacteria Centrifugation Food Infection Institutional Animal Care and Use Committees Isoflurane Lung Mus Pathologic Processes Saline Solution Skin Staphylococcus Staphylococcus aureus Strains Subcutaneous Injections Tissues trypticase-soy broth Vaccination Woman

Most recents protocols related to «Trypticase»

1
Preparation of Trypticase Soy Broth
According to the manufacturer’s instructions, 30 grams of Trypticase soy broth (TSB) powder (Merck, Germany) was completely dissolved in one litre of distilled water; then, it was heated and boiled for one minute until the powder was completely dissolved. After distribution inside the plates, it was autoclaved at 121°C for 15 minutes and finally kept in the refrigerator.
Golfakhrabadi F., Mansouri D., Montazeri E.A., Salimi A., Babadi F, & Rakhshan V. (2024). Preparing and evaluating the anti-microbial effect of Allium jesdianum mouthwash on some of the most common oral microorganisms. Journal of Family Medicine and Primary Care, 13(2), 640-646.
Publication 2024
2
Salmonella enterica Culturing Protocol
The Salmonella enterica subsp. entericaserotypes used in this study are listed in Table 1. Stock cultures of the serotypes were kept frozen (− 80°C) in 20% glycerol. Initially, each strain was grown on Trypticase Soy Agar plates (TSA; Becton, Dickinson and Company, Sparks, MD) or/and Trypticase Soy Broth (TSB), overnight at 37°C, to ensure pure culture. The cultures grown overnight were used for inoculation.
Williams A., Gaoh S.D., Savenka A., Paredes A., Alusta P., Ahn Y, & Buzatu D.A. (2024). A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C. Frontiers in Microbiology, 15, 1342478.
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Publication 2024
3
Antioxidant Potential of Lagerstroemia Leaves

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Publication 2024
4
Cultivation and Viability Assessment of Trichomonas vaginalis
Not available on PMC !
We used T. vaginalis strain T106 was kindly provided by Prof. Jae Sook Ryu (Hanyang University). The strain was cultured in screw-capped glass tubes containing complex trypticase-yeast extract-maltose (TYM) medium. This medium consisted of trypticase peptone, yeast extract, maltose monohydrate, Lascorbic acid, L-cysteine, phosphates (i.e., monopotassium, and dipotassium phosphates), horse serum, and antibiotics (penicillin 100 U/ml, 100 µg/ml of streptomycin; Welgene, Kyungsan, Korea) (Table 1). T. vaginalis was incubated at 37℃ in a 5% CO 2 chamber with loosely capped tubes for ventilation. Viability of T. vaginalis was assessed using trypan blue staining.
Jo H., Shin S., Agura T., Ahn H., Jeong S., Kim Y., & Kang J.S. (2024). Alloferon regulates the growth and movement of Trichomonas vaginalis by altering hydrogenosomes.
Publication 2024
5
Culturing and Manipulating C. jejuni
C. jejuni NCTC 11168 (ATCC 700819) freezer stocks were revived onto 5% sheep's blood trypticase soy agar (TSA) plates that contained 10 µg/mL vancomycin and 5 µg/mL trimethoprim as background antibiotics along with 15 µg/mL chloramphenicol for the mlghB, mlghC, wcaG, and wcaG∆ mutants and 30 µg/mL kanamycin for the kpsM mutant. Unless stated otherwise, all incubations were done at 37°C in microaerobic conditions (85% humidity, 10% CO2, and 5% O2). After overnight incubation, the bacteria were collected in 1 mL trypticase soy broth (TSB), and 100 µL adjusted to an OD600 of 0.01 were spread on TSA background plates and incubated overnight, as above. The bacteria were collected in 1 mL TSB and diluted to a specific OD600 dependent on the subsequent experiment. Heat‐inactivation was performed at 57°C for 45 min with intermittent shaking.
Myles M., Barnawi H., Mahmoudpour M., Shlimon S., Chang A., Zimmermann D., Choi C., Zebian N, & Creuzenet C. (2024). Effect of the polysaccharide capsule and its heptose on the resistance of Campylobacter jejuni to innate immune defenses. MicrobiologyOpen, 13(1), e1400.
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Publication 2024

Top products related to «Trypticase»

1
Trypticase soy broth (tsb) by BD
Sourced in United States, Japan, Germany
Trypticase soy broth is a general-purpose culture medium used for the growth and cultivation of a wide range of microorganisms, including bacteria and fungi. It provides the necessary nutrients and growth factors required for the maintenance and proliferation of various microbial species.
2
Trypticase soy agar by BD
Sourced in United States, Germany, France
Trypticase soy agar is a general-purpose microbiological growth medium. It provides nutrients and support for the cultivation of a wide range of bacterial species. The medium is made from tryptic digest of casein and soy peptone, and is commonly used for the isolation, cultivation, and enumeration of various microorganisms.
3
Sheep blood by BD
Sourced in United States, Germany, France, Austria, Japan, Belgium, Switzerland, China
Sheep blood is a biological material derived from sheep. It is a complex fluid consisting of various cellular components and plasma. Sheep blood is commonly used as a component in specialized laboratory equipment and procedures, where its specific properties and composition are required for research, analysis, or testing purposes. The core function of sheep blood is to serve as a standardized biological material for these laboratory applications.
4
Trypticase soy broth (tsb) by Thermo Fisher Scientific
Sourced in United Kingdom, Italy, United States, France
Trypticase soy broth is a general-purpose microbiological growth medium used for the cultivation and enumeration of a wide range of microorganisms, including bacteria, yeasts, and fungi. It provides the necessary nutrients and growth factors for the proliferation of these organisms in a laboratory setting.
5
Trypticase soy broth (tsb) by Merck Group
Sourced in Germany, United States
Trypticase soy broth is a general-purpose culture medium used for the cultivation of a wide range of microorganisms. It provides the necessary nutrients and growth factors for the cultivation of various bacteria, yeasts, and fungi.
6
Trypticase soy broth tsb by BD
Sourced in United States, Germany, United Kingdom, Canada
Trypticase Soy Broth (TSB) is a general-purpose, nutrient-rich microbial growth medium. It provides a balanced source of carbon, nitrogen, minerals, and other essential nutrients to support the growth of a wide range of microorganisms. TSB is commonly used for cultivation, isolation, and maintenance of various bacterial and fungal species in laboratory settings.
7
Trypticase soy agar by Thermo Fisher Scientific
Sourced in United Kingdom, United States, Italy
Trypticase soy agar is a general-purpose microbiological growth medium used for the cultivation and enumeration of a wide variety of aerobic bacteria. It provides essential nutrients for the growth of bacteria and supports the formation of colony-forming units.
8
Tryptic soy broth (tsb) by BD
Sourced in United States, Germany, Canada, France, United Kingdom, China, Poland, Japan, Denmark, Cameroon, Switzerland, Italy
Tryptic soy broth is a general-purpose microbiological culture medium used for the cultivation and growth of a wide range of bacteria. It provides the necessary nutrients, including peptones, glucose, and salts, to support the growth of a variety of bacterial species.
9
Yeast extract by BD
Sourced in United States, Japan, Germany, United Kingdom, France, India, Poland, Mexico, Australia, Italy, Canada
Yeast extract is a powder or paste derived from the autolysis of yeast cells. It contains a variety of amino acids, vitamins, and other nutrients that can support the growth and propagation of microorganisms in laboratory settings.
10
Trypticase soy agar plate by BD
Sourced in United States
Trypticase Soy Agar plates are a microbiological growth medium used for the cultivation of a wide range of bacteria. The plates provide a nutritious agar-based environment that supports the growth and isolation of various bacterial species.

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