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UCP1 protein, human

The UCP1 (uncoupling protein 1) is a mitochondrial inner membrane protein that plays a key role in thermogenesis and energy expenditure.
It is primarily expressed in brown adipose tissue and is responsible for uncoupling oxidative phosphorylation, dissipating energy as heat rather than ATP production.
UCP1 is a critical regulator of body temperature and is implicated in various metabolic processes, including weight management and cold adaptation.
Understanding the expression, regulation, and function of UCP1 is crucial for researchers investigating energy homeostasis, obesity, and related metabolic disorders.
This MeSH term provides a concise overview of the UCP1 protein and its physiological significance.

Most cited protocols related to «UCP1 protein, human»

1
Analyzing Brown Adipose Tissue in PET-CT Scans
Cited 28 times
We analyzed 3640 consecutive 18F-FDG PET–CT whole-body scans performed on 1972 patients for a variety of diagnostic reasons at Beth Israel Deaconess Medical Center from August 2003 through May 2006. Data on age and sex were obtained for all patients. Data on height, weight, fasting plasma glucose level, medication use, diagnosis, and smoking history were obtained for all patients who had substantial amounts of brown adipose tissue according to PET–CT scans and for a control group consisting of two patients without detectable brown adipose tissue who underwent scanning on the same day as each patient with brown adipose tissue (see Table 1 in the Supplementary Appendix, available with the full text of this article at NEJM.org). Outdoor temperatures in Boston for the dates of scans were obtained from the U.S. Weather Service.
Routine histologic and immunohistochemical assays for UCP1 with the use of polyclonal anti-mouse UCP1 antibody (Santa Cruz Biotechnology) were performed on paraffin sections. PET–CT images were acquired with the use of a Discovery LS multidetector helical PET–CT scanner (GE Medical Systems).20 (link) In areas where uptake of 18F-FDG was identified by PET and the presence of fat was identified by CT, the maximal and mean standardized uptake values (SUVs), defined as the activity per milliliter within the region of interest divided by the injected dose in megabecquerels per gram of body weight, were determined. Calculations were performed with the use of OpenPACS and PET–CT Viewer shareware.21 (link) Images from Massachusetts General Hospital were acquired in a similar manner.22 (link)
Cypess A.M., Lehman S., Williams G., Tal I., Rodman D., Goldfine A.B., Kuo F.C., Palmer E.L., Tseng Y.H., Doria A., Kolodny G.M, & Kahn C.R. (2009). Identification and Importance of Brown Adipose Tissue in Adult Humans. The New England journal of medicine, 360(15), 1509-1517.
Publication 2009
Antibodies, Anti-Idiotypic Biological Assay Body Weight Brown Fat Diagnosis F18, Fluorodeoxyglucose Glucose Mice, House Paraffin Patients Pharmaceutical Preparations Plasma Radionuclide Imaging Scan, CT PET Tomography, Spiral Computed UCP1 protein, human Whole Body Imaging
2
Evaluating Obesity-Associated Genetic Variants
Cited 16 times
We selected 74 candidate genes previously tested for association with obesity in humans [53 (link)]. For each gene, we first evaluated the ability of the Affymetrix SNPs to tag common SNPs (MAF > 0.05) within +/− 5 kb of the gene (r2 > 0.50 or r2 > 0.80) using the HapMap CEU database [54 (link)]. We then evaluated evidence for association using all Affymetrix SNPs within each gene as well as neighboring Affymetrix SNPs that could be used to improve coverage (r2 > 0.5). For each gene, we report coverage statistics as well as the SNP that showed strongest evidence for association.
We selected 74 genes that were previously targeted in associations studies aiming to identify genetic determinants of obesity in humans [53 (link)]: ACE, ACTN, ADIPOQ, ADIPOR1, ADIPOR2, ADRB1, ADRB2, AGER, AHSG, APOA2, APOA4, APOA5, AR, BDNF, CASQ1, COL1A1, COMT, CRP, CYP11B2, DIO1, ENPP1, ESR1, ESR2, FABP2, FOXC2, GAD2, GFPT1, GHRHR, GNAS, GNB3, GPR40, H6PD, HSD11B1, HTR2C, ICAM1, IGF1, IGF2, IL6, IL6R, KCNJ11, KL, LEP, LEPR, LIPC, LPL, LTA, MC4R, MCHR1, MKKS, MTHFR, MTTP, NMB, NOS3, NPY, NPY2R, NR0B2, NTRK2, PARD6A, PLIN, PPARG, PPARGC1A, PRDM2, PTPN1, PYY, RETN, SCD, SELE, SERPINE1, TAS2R38, TNF, UCP1, UCP2, UCP3, and VDR. We did not consider genes associated with drug-induced body weight gain or mitochondrial genes [53 (link)].
The following genes have previously been investigated for their role in obesity and related traits but are not well tagged by SNPs in the Affymetrix array: ADRB3, DRD4, INS, and APOE.
Scuteri A., Sanna S., Chen W.M., Uda M., Albai G., Strait J., Najjar S., Nagaraja R., Orrú M., Usala G., Dei M., Lai S., Maschio A., Busonero F., Mulas A., Ehret G.B., Fink A.A., Weder A.B., Cooper R.S., Galan P., Chakravarti A., Schlessinger D., Cao A., Lakatta E, & Abecasis G.R. (2007). Genome-Wide Association Scan Shows Genetic Variants in the FTO Gene Are Associated with Obesity-Related Traits. PLoS Genetics, 3(7), e115.
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Publication 2007
ADRB1 protein, human ADRB2 protein, human APOA4 protein, human ApoE protein, human COMT protein, human DRD4 protein, human FABP2 protein, human Genes Genes, Mitochondrial GNB3 protein, human HapMap Homo sapiens IGF1 protein, human IL6R protein, human insulin-like growth factor 2, human Intercellular Adhesion Molecule-1 Kaufman-Mckusick Syndrome leptin receptor, human LIPC protein, human MC4R protein, human Methylenetetrahydrofolate Reductase NOS3 protein, human O(4)-methylthymidine triphosphate Obesity Pharmaceutical Preparations PPARGC1A protein, human PRDM2 protein, human PTPN1 protein, human RAGE receptor protein, human Reproduction SERPINE1 protein, human Single Nucleotide Polymorphism tropomyosin-related kinase-B, human UCP1 protein, human
3
Regulation of Ucp1 Promoter Activity
Cited 13 times

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Publication 2014
Adipocytes Adipogenesis Biological Assay Cells Cloning Vectors Galactosidase interferon regulatory factor 4, human Luciferases Luciferases, Renilla Mus Paragangliomas 3 Plasmids PPARGC1A protein, human Promega Transfection Trypsin UCP1 protein, human
4
Western Blot Analysis of Metabolic Proteins
Cited 10 times

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Publication 2017
Actins alpha-Tubulin Antibodies Biological Assay Buffers Conserved Helix-Loop-Helix Ubiquitous Kinase DDIT3 protein, human Densitometry Diagnosis Edetic Acid Egtazic Acid Glucose Regulated Protein 78 kDa Goat Hypothalamus Immunoglobulins Liver Mus Muscle Tissue Orthovanadate polyvinylidene fluoride Protease Inhibitors Protein Kinase C-epsilon Proteins Rabbits SDS-PAGE Sodium Sodium Fluoride sodium pyrophosphate Sucrose Tissue, Membrane Tissues Transcription Factor RelA Triton X-100 Tromethamine UCP1 protein, human X-Ray Film
5
Adipose Tissue Histology and Immunostaining
Cited 10 times

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Publication 2016
Antibodies Antigens Dehydration Eosin Fluorescent Antibody Technique Furuncles Microwaves Paraffin Embedding Sodium Citrate Tissue, Adipose Tissues UCP1 protein, human

Most recents protocols related to «UCP1 protein, human»

1
Adipose Tissue Histological Analysis
The adipose tissue was quickly removed from the mice, washed with normal saline, dried and then fixed with 10% formalin. After rapid removal of the adipose tissue, it was placed in 4% paraformaldehyde. It was then dehydrated in an ascending series of ethanol, and equilibrated with xylene, followed by embedding in paraffin and sectioning into 5–10 µm slices. Then, the samples were dewaxed with xylene and a descending series of ethanol. Continued sections were stained with both Mayer’s hematoxylin and eosin (HE).
Dried paraffin sections were dewaxed and hydrated and then closed with 1% BSA for 30 min, the closure solution was blotted dry on blotting paper. Each section was incubated with UCP1 antibody (abcam1:100) overnight at 4°C in a wet box, and washed 3 times with PBS. After aspirating the residual liquid, the sections were incubated with fluorescent secondary antibody (FITC1:100) for 1 h at room temperature in a wet box protected from light, then rinse 3 times with PBS, the excess liquid was aspirated. A small amount of anti-fluorescence quencher containing DAPI dropwise was added, the sample was covered with a coverslip, and stored in a wet box protected from light for observation under a fluorescence microscope for photographs.
Lv Y., Lv X., Feng J., Cheng F., Yu Z., Guan F, & Chen L. (2023). (20R)-panaxadiol improves obesity by promoting white fat beigeing. Frontiers in Pharmacology, 14, 1071516.
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Publication 2023
DAPI Edema Eosin Ethanol Fluorescence Fluorescent Antibody Technique Formalin Hematoxylin Immunoglobulins Light Microscopy, Fluorescence Mus Normal Saline Paraffin paraform Tissue, Adipose UCP1 protein, human Xylene
2
Western Blot Analysis of Thermogenic Proteins
Proteins were extracted from cell lysates following the manufacturer’s protocols (Beyotime, China). Protein concentration was quantified using the BCA protein assay kit (Thermo Fisher Scientific, United States) and 30 μg protein was separated in a 12% SDS polyacrylamide gel and electro transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad, United States). Membranes were blocked with 5% (w/v) BSA for 2 h at room temperature and then incubated with primary antibodies with light shaking overnight at 4°C. Primary antibodies against UCP1, PGC-1α, TFAM, NRF1, CREB, P-CREB and GAPDH (abcam) were diluted to a ratio of 1:1,000 in TBST buffer. The membranes were washed 3 times for 5 min each with 10 mL of TBST [10 mM Tris-HCl, 150 mM NaCl and 0.1% (v/v) Tween-20] and then incubated with secondary antibody at room temperature for 2 h. Secondary antibodies goat anti-rabbit or goat anti-mouse (Proteintech, United States) were diluted to a ratio of 1:5,000 in TBST buffer. The membrane was incubated in Western ECL substrate (Thermo fisher or Proteintech, United States) and exposed to Tanon imager, using ImageJ software for image analyses.
Lv Y., Lv X., Feng J., Cheng F., Yu Z., Guan F, & Chen L. (2023). (20R)-panaxadiol improves obesity by promoting white fat beigeing. Frontiers in Pharmacology, 14, 1071516.
Full text: Click here
Publication 2023
Antibodies Biological Assay Buffers GAPDH protein, human Goat Immunoglobulins Mus polyacrylamide gels polyvinylidene fluoride PPARGC1A protein, human Proteins Rabbits Sodium Chloride TFAM protein, human Tissue, Membrane TNFSF14 protein, human Tromethamine Tween 20 UCP1 protein, human
3
Pharmacological Inhibition of MVA Pathway

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Publication 2023
Adipocytes Albinism Asian Persons Corn oil Diet Embryo Females Fetus HMGCR protein, human Isoflurane Males Mice, House Mice, Inbred C57BL mitogen-activated protein kinase 3, human Psychological Inhibition Rivers Tamoxifen UCP1 protein, human Yeasts
4
Western Blot Analysis of Protein Expression

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Publication 2023
Actins alpha-Tubulin anti-IgG Antibodies Biological Factors Caspase 3 Chemiluminescence Common Cold Goat Histone H3 HMGCR protein, human Immobilon Immobilon P Immune Sera Immunoglobulins Mus Novus polyvinylidene fluoride Proteins Rabbits Rattus SDS-PAGE Tissue, Membrane UCP1 protein, human Western Blotting
5
Differentiation and Thermogenic Activation of Immortalized Brown Preadipocytes

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Publication 2023
1-Methyl-3-isobutylxanthine Adipocytes, Brown Cell Culture Techniques Cells Indomethacin Insulin Mice, Transgenic Rosiglitazone UCP1 protein, human

Top products related to «UCP1 protein, human»

1
Ab10983 by Abcam
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Ab10983 is a primary antibody used for protein detection in various research applications. It is designed to target a specific epitope of the target protein. The antibody is produced in a host species and purified using standard techniques.
2
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
3
Anti ucp1 by Abcam
Sourced in United States, United Kingdom
Anti-UCP1 is a primary antibody that specifically recognizes the Uncoupling Protein 1 (UCP1), also known as thermogenin, which is a mitochondrial inner membrane protein primarily expressed in brown adipose tissue. This antibody can be used to detect and quantify UCP1 expression in various experimental systems.
4
Trizol by Thermo Fisher Scientific
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TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
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The High-Capacity cDNA Reverse Transcription Kit is a laboratory tool used to convert RNA into complementary DNA (cDNA) molecules. It provides a reliable and efficient method for performing reverse transcription, a fundamental step in various molecular biology applications.
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β-actin is a protein that is found in all eukaryotic cells and is involved in the structure and function of the cytoskeleton. It is a key component of the actin filaments that make up the cytoskeleton and plays a critical role in cell motility, cell division, and other cellular processes.
8
Anti ucp1 antibody by Abcam
Sourced in United States, United Kingdom
The Anti-UCP1 antibody is a laboratory reagent used to detect and quantify the expression of the Uncoupling Protein 1 (UCP1) in biological samples. UCP1 is a mitochondrial protein primarily found in brown adipose tissue and is involved in thermogenesis. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of UCP1 in research settings.
9
Rneasy mini kit by Qiagen
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
10
Ab23841 by Abcam
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Ab23841 is a mouse monoclonal antibody that targets a specific protein. This antibody is intended for use in research applications.

More about "UCP1 protein, human"

Discover the power of the UCP1 (Uncoupling Protein 1) in regulating thermogenesis and energy expenditure.
This crucial mitochondrial inner membrane protein is primarily expressed in brown adipose tissue and plays a vital role in uncoupling oxidative phosphorylation, dissipating energy as heat rather than ATP production.
Understanding the expression, regulation, and function of UCP1 is crucial for researchers investigating energy homeostasis, obesity, and related metabolic disorders.
Explore the wide range of UCP1-related research tools and techniques, including the use of Ab10983 and Anti-UCP1 antibodies for protein detection, TRIzol and RNeasy Mini Kit for RNA extraction, and the High-Capacity cDNA Reverse Transcription Kit for gene expression analysis.
Leverage the power of PVDF membranes and β-actin as a reference protein to ensure accurate and reliable UCP1 protein quantification.
Stay on the cutting edge of UCP1 research with the AI-driven platform PubCompare.ai.
This innovative tool helps you locate the best protocols from literature, preprints, and patents, while providing data-driven insights to enhance the reproducibility and accuracy of your studies.
Discover the future of scientific research and take your UCP1 protein investigations to new heights with PubCompare.ai's intuitive interface and data-rich resources.
Whether you're exploring the role of UCP1 in thermogenesis, investigating its potential in weight management, or studying its involvement in cold adaptation, this comprehensive overview provides a solid foundation to elevate your research.
Empower your UCP1 studies with the right tools, techniques, and insights to uncover the secrets of this intriguing mitochondrial protein.