> Chemicals & Drugs > Amino Acid > Vitronectin

Vitronectin

Vitronectin is a multifunctional glycoprotein found in the extracellular matrix and blood plasma.
It plays a key role in cell adhesion, migration, and tissue repair processes.
Vitronectin interacts with various cell surface receptors, including integrins, and regulates diverse biological functions such as coagulation, fibrinolysis, and complement activation.
Understanding the molecular mechanisms and physiological roles of vitronectin is crucial for research in areas like wound healing, tissue engineering, and the development of targeted therapies.
This concize overview provides a foundational understanding of this important extracellular matrix component.

Most cited protocols related to «Vitronectin»

Reprogramming Fibroblasts to iPSCs

Cited 77 times

A schematic diagram of the procedure is described in Figure 4. Episomal plasmids and methods were described previously^{15 (link)}. Plasmid combination #19 (pEP4-E-O2S-E-T2K, pEP4-E-O2S-E-N2K and pCEP4M2L) was used for most reprogramming unless mentioned otherwise. Plasmids and EBNA mRNA were electroporated into fibroblast cells on Amaxa apparatus according to company instructions. One million cells were used in each electroporation, which were then plated into two 6-well plates. E8 + hydrocortisone media were used for the first 5–10 days, according to cell survival and proliferation after electroporation. When confluency was reached ~20%, hydrocortisone was removed. ES-like iPS cell colonies usually appear after ~25 days. Cells were then picked into individual wells with E8 (TGFβ or NODAL). Cells were passaged for ~ 15 passages before subcloning with Y27632 on Matrigel or vitronectin.

Chen G., Gulbranson D.R., Hou Z., Bolin J.M., Ruotti V., Probasco M.D., Smuga-Otto K., Howden S.E., Diol N.R., Propson N.E., Wagner R., Lee G.O., Antosiewicz-Bourget J., Teng J.M, & Thomson J.A. (2011). Chemically defined conditions for human iPS cell derivation and culture. Nature methods, 8(5), 424-429.

Publication 2011

Cells Cell Survival Electroporation Episomes Fibroblasts Hydrocortisone Induced Pluripotent Stem Cells matrigel Plasmids RNA, Messenger Transforming Growth Factor beta Vitronectin Y 27632

Episomal Reprogramming of Fibroblasts to iPSCs

Cited 61 times

Episomal plasmids and methods were described previously^{15 (link)}. Plasmid combination #19 (pEP4-E-O2S-E-T2K, pEP4-E-O2S-E-N2K and pCEP4M2L) was used for most reprogramming unless mentioned otherwise. Plasmids and EBNA mRNA were electroporated into fibroblast cells on Amaxa apparatus according to company instructions. One million cells were used in each electroporation, which were then plated into two 6-well plates. E8 + hydrocortisone media were used for the first 5–10 days, according to cell survival and proliferation after electroporation. When confluency was reached ~20%, hydrocortisone was removed. ES-like iPS cell colonies usually appear after ~25 days. Cells were then picked into individual wells with E8 (TGFβ or NODAL). Cells were passaged for ~ 15 passages before subcloning with Y27632 on Matrigel or vitronectin.

Publication 2011

Cells Cell Survival Electroporation Episomes Fibroblasts Hydrocortisone Induced Pluripotent Stem Cells matrigel Plasmids RNA, Messenger Transforming Growth Factor beta Vitronectin Y 27632

Pluripotent Stem Cell Cardiac Differentiation

Cited 46 times

The following matrices were assessed for the ability to support pluripotent growth and subsequent cardiac differentiation: 9 µg/cm² growth-factor reduced Matrigel (1:200, Corning) in DMEM/F12; 625 ng/cm² vitronectin peptide (Synthemax II-SC, 1:320, Corning) in ultrapure water (1:50 also tested); 1 µg/cm² full length recombinant human vitronectin (1:50, Primorigen) in D-PBS with CaCl₂ and MgCl₂; 2.5 µg/cm² laminin-521 (1:80, Biolamina) in DPBS; 2 µg/cm² truncated recombinant human laminin-511 iMatrix-511 (1:50, Iwai North America, Foster City, CA, USA) in DPBS; 1 µg/cm² rH E-cadherin (1:25, StemAdhere, Primorigen/Stemcell Technologies); and 10 µg/cm² fibronectin (1:20, EMD Millipore) in D-PBS. All were used at 2 mL per well of a 6-well (9.6 cm²). Matrices were assessed on both 6-well polystyrene tissue culture plates and untreated plates (both from Greiner). Also tested were Synthemax-T 6-well plates, and fibronectin mimetic plates (both from Corning) and 10 µg/cm² Pronectin (Sigma-Aldrich).

Burridge P.W., Matsa E., Shukla P., Lin Z.C., Churko J.M., Ebert A.D., Lan F., Diecke S., Huber B., Mordwinkin N.M., Plews J.R., Abilez O.J., Cui B., Gold J.D, & Wu J.C. (2014). Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes. Nature methods, 11(8), 855-860.

Publication 2014

Cadherins FN1 protein, human Growth Factor Heart Homo sapiens Laminin laminin-511, human Magnesium Chloride matrigel Peptides Polystyrenes Stem Cells Tissues Vitronectin

Pluripotent Stem Cell Cardiac Differentiation

Cited 42 times

Publication 2014

Cadherins FN1 protein, human Growth Factor Heart Homo sapiens Laminin laminin-511, human Magnesium Chloride matrigel Peptides Polystyrenes Stem Cells Tissues Vitronectin

Feeder-Free Cortical Spheroid Generation from hiPSCs

Cited 12 times

hiPSCs were maintained on six-well plates coated with recombinant human
vitronectin (VTN-N, Life Technologies, A14700) in Essential 8™ medium
(Life Technologies, A1517001). To coat 6-well plate, 1 ml of vitronectin
(diluted at a 1:100 ratio with Dulbecco’s phosphate-buffered saline
(DPBS); Life Technologies, 14190) was added per well, and then incubated at room
temperature for 1 h. To passage hiPSC (80–90% confluency), cells were
rinsed with 3–4 ml DPBS per well, and then 1 ml of 0.5 mM EDTA (Life
Technologies, 15575) was added for 7 minutes at room temperature. After removing
the EDTA, 2 ml of pre-warmed complete Essential 8™ medium was added to
collect cells. The cell suspension was then diluted in Essential 8™
medium (1:6–1:20 depending on the hiPSC line) and distributed in on
vitronectin-coated wells.
For the generation of 3D spheroids, hiPSCs were incubated with Accutase
(Innovate Cell Technologies, AT-104) at 37ºC for 7 minutes and
dissociated into single cells. Optionally, one day prior to spheroid formation,
hiPSCs can be exposed to 1% dimethyl sulfoxide (DMSO) in Essential 8™
medium. To obtain uniformly-sized spheroids, AggreWell™800 (Stemcell
Technologies, 34815) containing 300 microwells were used. Approximately 3
× 10⁶ single cells were added per AggreWell™800 well in
Essential 8™ medium supplemented with the ROCK inhibitor Y-27632 (10
μM, Selleckchem, S1049), centrifuged at 100 x g for 3 minutes to capture
the cells in the microwells and incubated at 37ºC with 5% CO₂.
After 24 hours, spheroids from each microwell were harvested by firmly pipetting
(with a cut the end of a P1000 tip) medium in the well up and down and
transferred into ultra-low attachment plastic dishes (Corning, 3262) in
Essential 6™ medium (Life Technologies, A1516401) supplemented with two
SMAD pathway inhibitors– dorsomorphin (2.5 μM, Sigma-Aldrich,
P5499) and SB-431542 (10 μM, Tocris, 1614). From day 2 to day 6,
Essential 6™ medium was changed every day and supplemented with
dorsomorphin and SB-431542. Optionally, the Wnt pathway inhibitor XAV-939 (2.5
μM, Tocris, 3748) can be added with the two SMAD pathway inhibitors. On
the sixth day in suspension, neural spheroids were transferred to neural medium
containing Neurobasal A (Life Technologies, 10888), B-27 Supplement without
vitamin A (Life Technologies, 12587) and GlutaMax (1:100, Life Technologies,
35050). The neural medium was supplemented with 20 ng/ml EGF (R&D Systems,
236-EG) and 20 ng/ml bFGF (R&D Systems, 233-FB) for 19 days (until day 24)
with daily medium change in the first 10 days, and every other day for the
subsequent 9 days. To promote differentiation of the neural progenitors into
neurons, the neural medium was supplemented with 20 ng/ml BDNF (Peprotech,
450–02) and 20 ng/ml NT3 (Peprotech, 450–03) with medium changes
every other day. From day 43, only neural medium without growth factors was used
for medium changes every 4 days.
A step-by-step protocol for the generation cortical spheroids from hiPSC hiPSCs
in feeder-free, xeno-free conditions is available in Protocol Exchange (ref. 20 (link)).

Yoon S.J., Elahi L.S., Pașca A.M., Marton R.M., Gordon A., Revah O., Miura Y., Walczak E.M., Holdgate G.M., Fan H.C., Huguenard J.R., Geschwind D.H, & Pașca S.P. (2018). Reliability of human 3D cortical organoid generation. Nature methods, 16(1), 75-78.

Publication 2018

4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide accutase Cells Cortex, Cerebral dorsomorphin Edetic Acid Human Induced Pluripotent Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal inhibitors Nerve Growth Factors Nervousness Phosphates Saline Solution Sulfoxide, Dimethyl Vitronectin Wnt Signaling Pathway Y 27632

Most recents protocols related to «Vitronectin»

Histological Analysis and NK Cell Sorting

For histological analysis, the following primary antibodies (Abs) were employed: anti-vitronectin Abs (ab45139, Abcam, Cambridge, MA, and MAB38751, R&D Systems, Inc., Minneapolis, MN), anti-thrombospondin 1 Abs (Ab-11, Thermo Fisher Bio-scientific, Hudson, NH, and ab1823, Abcam), anti-CD45 Abs (ab10558, Abcam, and M0701, DAKO, Carpinteria, CA, USA). Additionally, for B220⁺CD11c⁺NK1.1⁺ NK cell sorting, the following antibodies were used; PE/Cy7 anti-mouse B220 (103222, BioLegends), PE anti-mouse CD11c (117308, BioLegends), APC anti-mouse NK1.1 (108710, BioLegends), and those isotype control antibodies, PE/Cy7 Rat IgG2a, κ (400521, BioLegends), PE American Hamster IgG (12-4888-81, Thermo Fisher), APC Mouse IgG2a κ (550882, BD Biosciences).

Yin C., Kato M., Tomita T., Han Y, & Hiratsuka S. (2023). Suppression of CEBPδ recovers exhaustion in anti-metastatic immune cells. Scientific Reports, 13, 3903.

Full text: Click here

Publication 2023

Anti-Antibodies Antibodies Hamsters IgG2A Immunoglobulin Isotypes Mus Thrombospondin 1 Vitronectin

Immunocytochemical Analysis of ECM Proteins

MSCs were washed and fixed with 40 g/L PFA for 30 min at RT. Cells were permeabilized with 0.1% (v/v) Triton X‐100 (Sigma-Aldrich) for 10 min, at RT, rinsed and blocked with 5 g/L BSA/ 0.1% (v/v) Triton X‐100 for 1 h at RT. Then, cells were incubated with primary antibody against collagen type I (COL1, 1:250, Rockland), osteopontin (OPN; 1:250, Santa Cruz Biotechnology), fibrillin-1 (FBN1; 1:100, Thermo Fisher Scientific), collagen Triple Helix Repeat Containing 1 (CTHRC1; 1:50, Santa Cruz Biotechnology) and vitronectin (VTN; 1:25, Santa Cruz Biotechnology) overnight at 4 °C. Cells were washed three times with PBS 1x, 5 min each, incubated with the respective secondary antibody (1:1 000; Thermo Fisher Scientific) for 1 h at RT and washed again. Cell nuclei were stained with 1 μg/mL DAPI (Invitrogen) for 5 min. The antibodies manufacturers and respective dilutions are detailed in Additional file 1: Table SIV. The stainings were visualized under the Leica DMi6000 FFW (Leica Microsystems). Cells incubated with the antibody diluent alone (without primary antibody), followed by incubation with the secondary antibody, were used as negative controls. Semi-quantification of the protein was performed using the ImageJ Fiji software, considering a minimum of 6 distinct fields per condition.

Moura S.R., Freitas J., Ribeiro-Machado C., Lopes J., Neves N., Canhão H., Rodrigues A.M., Barbosa M.A, & Almeida M.I. (2023). Long non-coding RNA H19 regulates matrisome signature and impacts cell behavior on MSC-engineered extracellular matrices. Stem Cell Research & Therapy, 14, 37.

Full text: Click here

Publication 2023

Antibodies Cell Nucleus Cells Collagen Collagen Type I DAPI Fibrillin-1 Helix (Snails) Immunoglobulins Osteopontin Proteins Staining Technique, Dilution Triton X-100 Vitronectin

Generation of Sensory Neurons from hiPSCs

The human iPSC line was manufactured and characterized at Anatomic Incorporated facilities, with informed consent, using proprietary technologies. hiPSCs were differentiated into sensory neurons using a commercially available kit, Senso-DM, according to the manufacturer’s instructions (Anatomic Incorporated, #7007). Briefly, undifferentiated human induced pluripotent stem cell line #1 (hiPSC; from female donor’s umbilical cord blood) was maintained with TeSR™-E8™ (STEMCELL Technologies Inc., 05990) on tissue culture-treated plastic that was coated with truncated recombinant human vitronectin (ThermoFisher Scientific # A14700). hiPSCs were dissociated using EDTA (0.55 mM) and single cell-seeded into TeSR-E8 supplemented with 10 µM Y-27632 (Selleckchem S1049) in flasks pre-coated with diluted 1:100 Matrix 1 in dPBS with Ca²⁺ and Mg²⁺ (ThermoFisher Scientific # 14040-133) to induce development into primal ectoderm^{68 (link)}. Subsequent daily media changes of Senso-DM 1-7 generated a population of immature sensory neurons, which were cryopreserved and later thawed for studies. Sensory neurons were maintained in culture at 37 °C with 5% CO₂ on glass coverslips coated with Poly-L-Lysine and Matrix 3 (Anatomic Incorporated, #M8003) in Senso-MM media (Anatomic Incorporated, #7008). Human iPSC-derived sensory neurons were incubated in culture for 14 days before recording. For siRNA-mediated knockdown assays, sensory neurons were co-transfected with siGLO Green Transfection Indicator (Dharmacon) and siRNA for UBE3A, PIEZO2, or the silencer negative control (scrambled siRNA; described above), using the Lipofectamine® RNAiMAX Transfection Reagent (ThermoFisher Scientific), according to the manufacturer’s protocols, 24 h before electrophysiological recording. The transfections were performed using antibiotic-free media.

Romero L.O., Caires R., Kaitlyn Victor A., Ramirez J., Sierra-Valdez F.J., Walsh P., Truong V., Lee J., Mayor U., Reiter L.T., Vásquez V, & Cordero-Morales J.F. (2023). Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome. Nature Communications, 14, 1167.

Full text: Click here

Publication 2023

Antibiotics Biological Assay Cells Edetic Acid Females Homo sapiens Human Induced Pluripotent Stem Cells Induced Pluripotent Stem Cells LINE-1 Elements Lipofectamine Lysine Poly A RNA, Small Interfering Sensory Receptor Cells Stem Cells Tissue Donors Tissues Transfection Umbilical Cord Blood Vitronectin Y 27632

Isogenic CRISPR-Cas9 Engineered hiPSC Lines

The BJFF.6 (BJFF) human iPSC line (Washington University Genome Engineering and iPSC Center [GEiC], St. Louis, MO), was used in this study as the isogenic WT control. CRISPR-Cas9 gene editing was used to create the V620I and T89I mutations in the BJFF cell line as described previously (Adkar et al., 2019 (link)). All three lines underwent STR profiling for cell line authentication and were verified to have no cross-contamination with other cell lines. All cells tested negative for mycoplasma. The hiPSCs were maintained on vitronectin (VTN-N; cat. num. A14700; Thermo Fisher Scientific, Waltham, MA)-coated plates in Essential 8 Flex medium (E8; cat. num. A2858501; Gibco, Thermo Fisher Scientific, Waltham, MA). Medium was changed daily until cells were passaged at 80–90% confluency (medium supplemented with Y-27632 [cat. num. 72304; STEMCELL Technologies, Vancouver, Canada] for 24 hr) or induced into mesodermal differentiation at 30–40% confluency.

Dicks A.R., Maksaev G.I., Harissa Z., Savadipour A., Tang R., Steward N., Liedtke W., Nichols C.G., Wu C.L, & Guilak F. (2023). Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes. eLife, 12, e71154.

Full text: Click here

Publication 2023

Cell Line Authentication Cell Lines Cells Clustered Regularly Interspaced Short Palindromic Repeats Genome Homo sapiens Human Induced Pluripotent Stem Cells Induced Pluripotent Stem Cells Mesoderm Mutation Mycoplasma Stem Cells Vitronectin Y 27632

Effects of Aβ and HIV-1 on Neural Progenitor Cells

HIV-1 stock was generated using human embryonic kidney (HEK) 293T cells (ATCC) transfected with pYK-JRCSF plasmid containing full-length proviral DNA. Throughout the study, HBMEC were exposed to HIV-1 particles at the p24 level of 30 ng/ml as previously reported [22 (link)]. Treatment was terminated by removing cell culture media containing HIV-1, followed by washing the cells with PBS.
Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS. Freshly solubilized Aβ solutions without pre-aggregation were used for experiments as such a form of Aβ was demonstrated to induce proinflammatory reactions [33 (link)]. Aβ (1–40) HiLyte was dissolved first in a basic buffer (0.1 M NH₄OH) and then diluted further in PBS as suggested by the manufacturer. Cells were treated with Aβ (1–40) or Aβ (1–40) HiLyte at the concentration of 100 nM in complete medium.
PAI039 (Tiplaxtinin, Catalog # PZ0295) was purchased from Millipore Sigma, Burlington, MA, USA). PAI039 is a potent and selective Serpine-1 inhibitor [34 (link)] and demonstrated efficacy in vivo in multiple models of acute arterial thrombosis. A 20 mM stock solution was prepared in DMSO. In a typical experiment, NPCs were cotreated with isolated EVs and/or 2 μM PAI039 for 24 h. Literature indicates that 1 μM PAI-039 can effectively inhibit Serpine-1 activity in vitro [35 (link)]. PAI039 exerts its activity by binding close to the vitronectin binding site [36 (link)].

András I.E., Serrano N., Djuraskovic I., Fattakhov N., Sun E, & Toborek M. (2023). Extracellular vesicle-Serpine-1 affects neural progenitor cell mitochondrial functions and synaptic density: modulation by amyloid beta and HIV-1. Research Square.

Full text: Click here

Publication Preprint 2023

Arteries Binding Sites Buffers Cardiac Arrest Cell Culture Techniques Cells Embryo HEK293 Cells HIV-1 Homo sapiens Kidney PAI 039 Plasmids Proviruses Sulfoxide, Dimethyl Thrombosis tiplaxtinin Vitronectin

Top products related to «Vitronectin»

Vitronectin by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Vitronectin is a glycoprotein that plays a role in cell adhesion, spreading, and migration. It is a component of the extracellular matrix and is involved in processes such as wound healing and tissue remodeling.

Essential 8 medium by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Australia

Essential 8 medium is a cell culture medium formulated for the maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It provides a defined, serum-free, and feeder-free environment for the culturing of these cell types.

Vitronectin xf by STEMCELL

Sourced in Canada, Germany, United States, United Kingdom

Vitronectin XF is a recombinant cell culture matrix protein. It functions as an extracellular matrix component to support the attachment and growth of various cell types in vitro.

Vitronectin by Merck Group

Sourced in United States, Germany, United Kingdom, Italy

Vitronectin is a glycoprotein that plays a role in cell adhesion and the regulation of the complement system. It is a component of the extracellular matrix and is involved in various biological processes.

Fibronectin by Merck Group

Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Austria, Switzerland, Macao, France, Italy, Australia, Ireland, India, Sao Tome and Principe, Hungary, Argentina

Fibronectin is an extracellular matrix glycoprotein that plays a role in cell adhesion, growth, migration, and differentiation. It is a key component of the cellular microenvironment and is involved in various biological processes.

Relesr by STEMCELL

Sourced in Canada, United States, United Kingdom

ReLeSR is a cell detachment solution designed for the gentle dissociation of various adherent cell types, including human pluripotent stem cells and primary cells. It is formulated to facilitate the enzymatic release of cells from culture vessels while preserving cell viability and functionality.

Tesr e8 medium by STEMCELL

Sourced in Canada, United States

TeSR-E8 is a serum-free, xeno-free, and defined medium for the culture of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). The medium is optimized to support the self-renewal and undifferentiated growth of these cells in vitro.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Laminin by Merck Group

Sourced in United States, Germany, United Kingdom, Canada, China, Italy, Switzerland, Israel, Sao Tome and Principe, France, Austria, Macao, Japan, India, Belgium, Denmark

Laminin is a protein component found in the extracellular matrix of cells. It plays a key role in cell attachment, differentiation, and migration processes.

Accutase by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Switzerland, Canada, Belgium, China, Sweden, Japan

Accutase is a cell detachment solution designed for the gentle dissociation of adherent cells. It contains a mixture of proteolytic and collagenolytic enzymes that effectively disrupt cell-cell and cell-matrix adhesions, allowing for the easy harvesting and passaging of a variety of cell types.

What are the different types or variations of Vitronectin?

Vitronectin exists in multiple forms and isoforms. The most common forms are the full-length, single-chain glycoprotein and a cleaved, two-chain version. Additionally, there are various post-translational modifications and splice variants that can impact Vitronectin's structure and function. Understanding these different forms is important when selecting the appropriate Vitronectin reagent for your research.

How can Vitronectin be utilized in tissue engineering and wound healing applications?

Vitronectin plays a crucial role in cell adhesion, migration, and tissue repair processes. It can be used as a substrate to promote cell attachment and proliferation in tissue engineering scaffolds and wound healing treatments. Vitronectin's ability to interact with integrins and other cell surface receptors makes it a valuable component for designing biomaterials that mimic the extracellular matrix and support tissue regeneration.

What are some common challenges in working with Vitronectin, and how can PubCompare.ai help?

One key challenge in Vitronectin research is identifying the most effective protocols from the vast literature. PubCompare.ai's AI-driven analysis can help researchers quickly pinoint the critical insights needed to choose the best protocols for their specific goals. The platfomr allows you to efficiently screen protocol literature and leverage AI to compare the effectiveness of different Vitronectin-related protocols, enabling you to select the most reproducible and accurate approach for your studies.

How can PubCompare.ai assist in optimizing Vitronectin-related research?

PubCompare.ai's intelligent comparison tools can help researchers working with Vitronectin in several ways. The platfomr allows you to quickly locate the most relevant protocols from literature, preprints, and patents, ensuring you have access to the latest and most effective methods. Additionally, the AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. This can save substantial time and effort, allowing you to focus on generating high-quality data and advancing your Vitronectin-related research.

More about "Vitronectin"

Vitronectin is a multifunctional glycoprotein that plays a crucial role in various biological processes.
This extracellular matrix (ECM) component is found in both the blood plasma and the extracellular environment.
Vitronectin is known to be involved in cell adhesion, migration, and tissue repair, making it an important factor in wound healing, tissue engineering, and the development of targeted therapies.
Vitronectin interacts with a variety of cell surface receptors, including integrins, and regulates diverse functions such as coagulation, fibrinolysis, and complement activation.
This versatile protein is essential for maintaining the integrity of the ECM and supporting the proper functioning of cells within the tissue microenvironment.
Understanding the molecular mechanisms and physiological roles of vitronectin is crucial for advancing research in areas like regenerative medicine.
Researchers often utilize vitronectin-based culture systems, such as Vitronectin XF and Essential 8 medium, to support the growth and differentiation of stem cells and other cell types.
Additionally, the related proteins fibronectin and laminin play important roles in the ECM and are commonly used in cell culture protocols, often in combination with vitronectin.
The cell dissociation reagent Accutase and the antibiotics penicillin/streptomycin are also frequently employed in cell culture procedures involving vitronectin and other ECM components.
By leveraging the insights gained from the MeSH term description and the metadescription, researchers can enhance their understanding of vitronectin and optimize their experimental protocols using AI-driven tools like PubCompare.ai.
This can lead to improved reproducibility, accuracy, and efficiency in their vitronectin-related research endeavors.