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> Chemicals & Drugs > Amino Acid > Zeocin

Zeocin

Zeocin is an antibiotic used in molecular biology and genetics research.
It is a selectable marker for identifying cells that have been successfully transfected or transformed with a desired gene.
Zeocin works by inhibiting protein synthesis, leading to cell death in untransfected cells.
Researchers can use Zeocin to isolate and cultivate cell lines that express a particular gene of interest.
This MeSh description provides a brief, informative overview of Zeocin and its applications in scientific research.
Optimized for SEO, this description can help researchers quickly understand the key features and uses of this important molecular biology tool.

Most cited protocols related to «Zeocin»

1
Generation of CD11c-Cre Transgenic Mice
Cited 113 times
To generate the CD11c-Cre transgene, the 160-kb mouse genomic BAC clone RP24-361C4 (BACPAC Resources) was modified by ET recombination, as previously described (43 (link)). The clone contains the entire Itgax (CD11c) gene but lacks the 5′ end of the adjacent Itgam (CD11b) gene, preventing the overexpression of the latter. The recombination cassette containing the Cre recombinase open reading frame, followed by the bovine growth hormone (BGH) polyA signal and the FRT site-flanked prokaryotic Zeocin resistance cassette (ZeoR), replaced the coding part of the first CD11c exon, and the ZeoR cassette was subsequently removed by FLP-mediated recombination. The clone insert was released from the vector backbone using NotI digestion, gel-purified, and microinjected into fertilized oocytes. The founder line containing two copies of the transgene (as determined by quantitative Southern hybridization) was chosen for further analysis. Mice were genotyped by genomic PCR using either generic Cre primers or primers specific for the CD11c-Cre transgene (5′-ACTTGGCAGCTGTCTCCAAG-3′ and 5′-GCGAACATCTTCAGGTTCTG-3′ were specific for the CD11c promoter and Cre, respectively).
The R26-EYFP strain (21 (link)) was provided by F. Costantini (Columbia University, New York, NY). The RBP-Jfl strain (19 (link)) was provided by L. Hennighausen (National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD), with permission from T. Honjo (Kyoto University, Kyoto, Japan). The Mx1-Cre strain was previously described (44 (link)). Cre-negative RBP-Jfl/fl littermates of CKO (RBP-Jfl/fl Cre+) mice were used as controls; in preliminary experiments, wild-type CD11c-Cre+ mice were used as controls and were found indistinguishable from CD11c-Cre− animals. For inducible RBP-J deletion, adult RBP-Jfl/fl Mx1-Cre+ or control RBP-Jfl/fl mice were injected with 0.25 mg poly(I):(C) three times, with 2-d intervals, and analyzed 3 wk later. For hematopoietic reconstitution, 3 × 106 total BM cells per mouse were injected i.v. into lethally irradiated C57BL/6 mice congenic for CD45.1. The recipient mice were analyzed 4–5 wk after reconstitution. Mice were maintained in a specific pathogen-free facility and used according to the protocol approved by the Columbia University's Institutional Animal Care and Use Committee.
Caton M.L., Smith-Raska M.R, & Reizis B. (2007). Notch–RBP-J signaling controls the homeostasis of CD8− dendritic cells in the spleen. The Journal of Experimental Medicine, 204(7), 1653-1664.
Publication 2007
Adult Animals Cells Clone Cells Cloning Vectors Cre recombinase Crossbreeding Deletion Mutation Diabetes Mellitus Digestion Digestive System Exons Generic Drugs Genes Genome growth hormone, bovine Hematopoietic System Institutional Animal Care and Use Committees ITGAM protein, human Kidney Diseases Mice, Inbred C57BL Mice, Laboratory Oligonucleotide Primers Ovum Poly A Poly I-C Prokaryotic Cells RBPJ protein, human Recombination, Genetic Specific Pathogen Free Strains Transgenes Vertebral Column Zeocin
2
Efficient Transduction and Selection Protocols
Cited 58 times
Cells were transduced at a MOI between 0.5 and 1. Viruses and cells were incubated overnight in D-MEM media (Invitrogen) containing 6 µg/ml of polybrene (Sigma) in a final volume of 950 µl for 6-well plates, 5 ml for 10 cm dishes and 11 ml for 15 cm dishes. The next day, the viruses were removed, the cells were rinsed twice with PBS and fresh media was added. For primary cells, a second round of transduction was done. Drug selection was added at 48 h post-transduction. The following concentrations were used: blasticidin: 2.5 µg/ml for WI38, HCA2, BJ cells and 5 µg/ml for the other cells, hygromycin: 100 µg/ml for WI38, HCA2, BJ cells, 200 µg/ml for U2OS cells and 300 µg/ml for HT1080 cells, neomycin: 300 µg/ml for WI38, HCA2, BJ cells and 800 µg/ml for the other cells, puromycin: 0.5 µg/ml for HeLa cells and 2.0 µg/ml for other cells, zeocin: 400 µg/ml for HT1080 cells and 200 µg/ml for other cells. Induction of the cDNA/shRNA/miRNA was typically done by addition of doxycycline at a final concentration of 1.0 µg/ml for 48 h (cDNA) or 96 h (shRNA/miRNA) unless indicated otherwise.
Campeau E., Ruhl V.E., Rodier F., Smith C.L., Rahmberg B.L., Fuss J.O., Campisi J., Yaswen P., Cooper P.K, & Kaufman P.D. (2009). A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. PLoS ONE, 4(8), e6529.
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Publication 2009
Cells DNA, Complementary Doxycycline HeLa Cells hygromycin A Hyperostosis, Diffuse Idiopathic Skeletal MicroRNAs Neomycin Pharmaceutical Preparations Polybrene Puromycin Short Hairpin RNA Virus Zeocin
3
Versatile Lentiviral and Retroviral Vectors
Cited 56 times
General cloning techniques were used to generate each of the vectors. Detailed protocols are available upon request. All the lentiviral Destination vectors are derived from the p156RRL-sinPPT-CMV-GFP-PRE/Nhe I vector [82] (link), [83] (link) and all the retroviral Destination vectors are derived from the pQCXI series (Clontech). For the lentiviral vectors, a linker was inserted between the Kpn I and Eco RI sites to clone the drug selection cassettes following the Woodchuck post-transcriptional response element (WPRE). All Destination vectors were propagated in the E.coli strain DB3.1 (Invitrogen) under appropriate antibiotic selection (see plasmid maps for details). All Entry vectors were propagated in the E.coli strain TOP10F' (Invitrogen) under kanamycin selection. After the LR recombination reaction, lentiviral vectors were propagated in the E.coli strain Stbl3 (Invitrogen) and retroviral vectors in TOP10F', all under ampicillin selection, except for the pLenti CMV/TO Zeo DEST (zeocin).
Campeau E., Ruhl V.E., Rodier F., Smith C.L., Rahmberg B.L., Fuss J.O., Campisi J., Yaswen P., Cooper P.K, & Kaufman P.D. (2009). A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. PLoS ONE, 4(8), e6529.
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Publication 2009
Ampicillin Antibiotics Clone Cells Cloning Vectors Deoxyribonuclease EcoRI Escherichia coli Kanamycin Marmota Microtubule-Associated Proteins Pharmaceutical Preparations Plasmids Recombination, Genetic Response Elements Retroviridae Strains Transcription, Genetic Zeocin
4
Identifying Synthetic Lethal Interactions in A375 Cells
Cited 30 times
A375 cells stably integrated with dCas9-VP64 and MS2-p65-HSF1 were transduced with individual guides from the top screening hits of the Zeocin and Puromycin screens (13 genes total, 3 sgRNAs per gene) as well as available cDNA at an MOI of <0.2 as described above. Cells were selected for guide expression with Zeocin (Life Technologies) for 5 days and replated at low density (3 × 103 cells per well in a 96-well plate). A375 cells and A375 cells expressing dCas9-VP64 and MS2-p65-HSF1 were plated as controls. Different concentrations of PLX-4720 (2µM, 0.5µM, 0.15µM) or vehicle (DMSO) were added 3h after plating. Cells were treated with PLX-4720 for 4 days before cell viability was measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega). For qPCR quantification of target gene upregulation, cells were also plated at 5 DPI (3 × 104 cells per well in a 96-well plate) and harvested for mRNA 24h after plating.
Konermann S., Brigham M.D., Trevino A.E., Joung J., Abudayyeh O.O., Barcena C., Hsu P.D., Habib N., Gootenberg J.S., Nishimasu H., Nureki O, & Zhang F. (2014). Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature, 517(7536), 583-588.
Publication 2014
Cells Cell Survival DNA, Complementary Genes Luminescent Measurements PLX 4720 Promega Puromycin RNA, Messenger Sulfoxide, Dimethyl Up-Regulation (Physiology) Zeocin
5
Lamin A Complementation in MEFs
Cited 28 times
A human lamin A cDNA was subcloned into the pTracer-CMV vector (Invitrogen Corp.). The linearized vector was transfected into lamin A/C −/− mouse embryonic fibroblasts (MEFs). Stable clones were selected using Zeocin, according to the manufacturer's instructions and the clones pooled. Subsequent analysis showed that the cells in the pool were heterogeneous with regard to lamin A expression.
Sullivan T., Escalante-Alcalde D., Bhatt H., Anver M., Bhat N., Nagashima K., Stewart C.L, & Burke B. (1999). Loss of a-Type Lamin Expression Compromises Nuclear Envelope Integrity Leading to Muscular Dystrophy. The Journal of Cell Biology, 147(5), 913-920.
Publication 1999
Clone Cells Cloning Vectors DNA, Complementary Embryo Fibroblasts Genetic Heterogeneity Homo sapiens Lamin Type A LMNA protein, human Mus Zeocin

Most recents protocols related to «Zeocin»

1
Generation of Single-Chain Fc Fragments
The pFUSE-Fc plasmid (400 mg) was supplied by the Department of Pathology, Sapporo Medical University School of Medicine. The retroviral reprogramming plasmids pFUSE-hIgG1-Fc2 (InvivoGen, CA, U.S.A.) have been previously described (21 (link)). pFUSE-Fc2 (IL2ss) plasmids facilitate the secretion of Fc-Fusion proteins from pFUSE-Fc-transfected cells. To obtain single-chain Fc fragments of IgH and IgK, we followed the principle of PCR assembly (22 (link)), and the information of the inserted synthesized representative cDNA and the amplified primers are shown in Supplementary Table 1. Briefly, the plasmid was digested with EcoRV and NcoI (Takara Bio, Shiga, Japan), and the targeted IgH and IgK domains were obtained using a two-step overlapping PCR. The PCR product was subcloned into the digested plasmid using In-Fusion HD Cloning kits (Takara Bio); the In-Fusion reaction mixture was transformed into competent cells, and individual isolated colonies were picked from the culture plate. Plasmid DNA was isolated using a Plasmid DNA purification kit (Macherey-Nagel GmbH& Co.), and 2.5 μg of the DNA transfected into 293 T cells using Lipofectamine 3000 (Thermo Fisher Scientific, MS, U.S.A.). Subsequently, the 293 T cells were grown in Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum and 1% penicillin-streptomycin solution with zeocin (300 μg/mL, InvivoGen), and the supernatant was collected from the culture well on day 2 and days 7–10. IgGs from the supernatant were collected from 3–5 culture dishes and purified using a spin column-based antibody purification kit (Protein G; Cosmo Bio, Tokyo, Japan). The density of IgG was measured using the NanoDrop One (Thermo Fisher Scientific).
Iwabuchi S., Tsukahara T., Okayama T., Kitabatake M., Motobayashi H., Shichino S., Imafuku T., Yamaji K., Miyamoto K., Tamura S., Ueha S., Ito T., Murata S.I., Kondo T., Ikeo K., Suzuki Y., Matsushima K., Kohara M., Torigoe T., Yamaue H, & Hashimoto S. (2023). B cell receptor repertoire analysis from autopsy samples of COVID-19 patients. Frontiers in Immunology, 14, 1034978.
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Publication 2023
Cells DNA, Complementary Eagle Fetal Bovine Serum G-substrate HEK293 Cells Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulin Fc Fragments Immunoglobulins Lipofectamine Oligonucleotide Primers Penicillins Pharmaceutical Preparations Plasmids Proteins Retroviridae secretion Streptomycin Zeocin
2
Mycobacterial Growth Conditions and Fatty Acid Sensitivity
Mtb was grown at 37 °C in Difco Middlebrook 7H9 broth or on 7H10 agar supplemented with 0.2% glycerol (7H9) or 0.5% glycerol (7H10), 0.05% Tween-80, 1x oleic acid-albumin-dextrose-catalase (OADC) and the appropriate antibiotics, unless otherwise specified. Media for the ΔmacE strain and strains to be tested for fatty acid sensitivity or fatty acid-dependent phenotypes were similarly prepared except 0.05% tyloxapol was used instead of Tween-80, and fatty acid-free albumin-dextrose-catalase (ADC) was used instead of OADC. Where required, antibiotics or small molecules were used at the following concentrations: kanamycin at 20 μg/mL; anhydrotetracycline (ATc) at 100 ng/mL, hygromycin at 50 μg/mL, zeocin at 20 μg/mL, and V-59 at 10 µM. Mtb cultures were grown standing in tissue culture flasks (unless otherwise indicated) at 37 °C, 5% CO2. Fatty acid sensitivity testing on 7H10 agar was conducted with 500 μM oleic acid or 200 μM palmitic acid.
M. smegmatis was grown at 37 °C in similarly supplemented 7H9 broth or 7H10 agar except ADC was used instead of OADC.
Wong A.I., Beites T., Planck K.A., Fieweger R.A., Eckartt K.A., Li S., Poulton N.C., VanderVen B.C., Rhee K.Y., Schnappinger D., Ehrt S, & Rock J. (2023). Cyclic AMP is a critical mediator of intrinsic drug resistance and fatty acid metabolism in M. tuberculosis. eLife, 12, e81177.
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Publication 2023
Agar Albumins anhydrotetracycline Antibiotics, Antitubercular Catalase Fatty Acids Glucose Glycerin hygromycin A Hypersensitivity Kanamycin Oleic Acid Palmitic Acid Phenotype Strains Tissues Tween 80 tyloxapol Zeocin
3
Gene Disruption in Physcomitrella patens
Ppddm1 was obtained through the replacement of the gene coding region with Zeocin resistance antibiotic cassette through homologous recombination. Homologous flanking 5’ (1068bp upstream to ATG site) and 3’ (252 bp upstream to stop site and 536bp downstream to stop site) were amplified using KOD polymerase (Sigma-Aldrich) and cloned into a PMBL5-Zeo vector. Integration of flanking regions was validated using PCR. Linearized plasmid was introduced to moss via PEG-mediated transformation protocol [43 (link)]. After three days, the regenerated protoplasts were transferred to the BCDAT medium. After seven days the moss was subjected to selection using Zeocin (25ng\μl). After three weeks, surviving plants were subjected to PCR to validate the correct integration of the construct into the genome and gene replacement. This was done through primers targeting the endogenous gene sequence and primers targeting regions flanking the 5’ and 3’ homologous regions and Zeocin resistance cassette.
Griess O., Domb K., Katz A., Harris K.D., Heskiau K.G., Ohad N, & Zemach A. (2023). Knockout of DDM1 in Physcomitrium patens disrupts DNA methylation with a minute effect on transposon regulation and development. PLOS ONE, 18(3), e0279688.
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Publication 2023
Antibiotic Resistance, Microbial Cloning Vectors Genes Genome Homologous Recombination Mosses Oligonucleotide Primers Plants Plasmids Protoplasts Zeocin
4
Stable TREM2/DAP12 Overexpression in HEK293
Human TREM2/DAP12 is overexpressed in HEK293 cell line (RRID: CVCL_0045, ATCC: PTA-4488) by transfection of a dual promoter pBudCE4.1 vector driving expression of TREM2 under the CMV promoter and DAP12 the under the EF1a promoter.
Stable clones were isolated after zeocin selection (800 µg ml−1 for 10 days), and TREM2 was detected by flow cytometry (APC-conjugated rat anti-human/mouse-TREM2 monoclonal antibody, R&D Systems, MAB17291). The clone showing the highest TREM2 expression level was selected.
van Lengerich B., Zhan L., Xia D., Chan D., Joy D., Park J.I., Tatarakis D., Calvert M., Hummel S., Lianoglou S., Pizzo M.E., Prorok R., Thomsen E., Bartos L.M., Beumers P., Capell A., Davis S.S., de Weerd L., Dugas J.C., Duque J., Earr T., Gadkar K., Giese T., Gill A., Gnörich J., Ha C., Kannuswamy M., Kim D.J., Kunte S.T., Kunze L.H., Lac D., Lechtenberg K., Leung A.W., Liang C.C., Lopez I., McQuade P., Modi A., Torres V.O., Nguyen H.N., Pesämaa I., Propson N., Reich M., Robles-Colmenares Y., Schlepckow K., Slemann L., Solanoy H., Suh J.H., Thorne R.G., Vieira C., Wind-Mark K., Xiong K., Zuchero Y.J., Diaz D., Dennis M.S., Huang F., Scearce-Levie K., Watts R.J., Haass C., Lewcock J.W., Di Paolo G., Brendel M., Sanchez P.E, & Monroe K.M. (2023). A TREM2-activating antibody with a blood–brain barrier transport vehicle enhances microglial metabolism in Alzheimer’s disease models. Nature Neuroscience, 26(3), 416-429.
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Publication 2023
Antibodies, Anti-Idiotypic Clone Cells Flow Cytometry Genetic Vectors HEK293 Cells Homo sapiens Monoclonal Antibodies Mus TREM2 protein, human Zeocin
5
Recombinant Vector Expression and Purification in Pichia pastoris
The recombinant vectors of Anman and mutants were linearized by restriction endonuclease Sal al restriction endonuclease. The linear vector was transferred into P. pastoris X33 and incubated in yeast extract peptone dextrose (YPD) solid medium containing 100 mg/mL bleomycin (zeocin) at 30°C for 72 h. Colonies were picked and incubated in 30 Ml YPD liquid medium at 30°C and 220 rpm for 24 h, and cells were then collected by centrifugation at 8,000 rpm and transferred to 30 mL YP medium containing 0.5% methanol at 28°C and 220 rpm for 72 h. Methanol was added every 12 h to maintain its concentration at 0.5% for induction of enzyme production.
The induced fermentation broth was centrifuged at 4°C at 8,000 rpm, and the collected supernatant was dialyzed for 16 h in 0.1 M NaH2PO4-Na2HPO4 containing 20 mM NaCl (pH 5.5, buffer A) to remove salt and metal ions. Anion exchange chromatography was used for the purification of crude enzymes. Briefly, after pre-equilibrating the anion exchange column (Hi Trap™ 1 mL Q HP) with Buffer A, the dialyzed crude enzyme was loaded onto the column, followed by a linear gradient elution using Buffer A with Buffer B (Buffer A with 1 M NaCl), and the collection containing the enzyme activity was utilized for the subsequent study. The purification of the samples was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli, 1970 (link)). The Bradford method was used to determine the protein concentration (Bradford et al., 1976 (link)).
Tan S., Tao X., Zheng P., Chen P., Yu X., Li N., Gao T, & Wu D. (2023). Thermostability modification of β-mannanase from Aspergillus niger via flexibility modification engineering. Frontiers in Microbiology, 14, 1119232.
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Publication 2023
Anions Bleomycin Buffers Cells Centrifugation Chromatography Cloning Vectors DNA Restriction Enzymes enzyme activity Enzyme Induction Enzymes Fermentation Glucose Ions Metals Methanol Peptones Proteins SDS-PAGE Sodium Chloride TRAP1 protein, human Yeast, Dried Zeocin

Top products related to «Zeocin»

1
Zeocin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Italy, Japan, China, Canada, Spain, Denmark, Switzerland, Australia
Zeocin is a selective antibiotic agent used for screening and selection of transformed cells. It acts as a lethal agent against non-transformed cells, allowing for the identification and isolation of successfully transformed cells.
2
Zeocin by InvivoGen
Sourced in United States, France, Japan, Germany, China
Zeocin is a selection antibiotic used for the stable transfection of mammalian and bacterial cells. It functions by inhibiting protein synthesis, leading to cell death in non-resistant cells.
3
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
6
Blasticidin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Italy, Spain, Switzerland, Austria, China
Blasticidin is a selection antibiotic used in cell biology research. It inhibits protein synthesis in eukaryotic cells, making it useful for selecting cells that have been successfully transfected or transduced with a gene of interest.
7
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
8
Blasticidin by InvivoGen
Sourced in United States, France, United Kingdom, Canada, Germany, China, Hong Kong
Blasticidin is a selection antibiotic used for the generation of stable cell lines. It inhibits protein synthesis by preventing peptide bond formation.
9
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
10
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

More about "Zeocin"

Zeocin is a versatile antibiotc widely used in molecular biology and genetics research.
It serves as a selectable marker, allowing researchers to identify cells that have been successfully transfected or transformed with a desired gene.
Zeocin works by inhibiting protein synthesis, leading to cell death in untransfected cells.
This makes it a powerful tool for isolating and cultivating cell lines that express a particular gene of interest.
In addition to Zeocin, other common reagents used in cell culture and transfection include Lipofectamine 2000 (a cationic lipid-based transfection reagent), fetal bovine serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM) for cell growth and maintenance, as well as antibiotics like Blasticidin, Penicillin, and Streptomycin for selection and contamination prevention.
Optimizing cell culture and transfection protocols is critical for acheiving reliable, reproducible results.
AI-driven comparisons, such as those offered by PubCompare.ai, can help researchers quickly identify the best protocols from literature, pre-prints, and patents, improving efficiency and accuracy.
By leveraging these tools, scientists can streamline their Zeocin-based research and focus on generating high-quality data.