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Anisomycin

Q: What are the main challenges researchers face when working with Anisomycin?

Due to Anisomycin's potent biological effects, it is important for researchers to have access to reliable and reproducible protocols for working with it. Ensuring the accuracy and reproducibility of Anisomycin studies can be challenging, as there may be variability in the protocols and methods used across different laboratories.

Q: Are there different types or variations of Anisomycin?

While Anisomycin is a single compound, there may be differnet commercial sources or preparations that researchers should be aware of. It's important to carefully characterize the specific Anisomycin product being used and ensure consistent quality acrsoss studies to maintain reproducibility.

Anisomycin is a natural product isolated from the bacterium Streptomyces griseolus.
It is a potent protein synthesis inhibitor that acts by blocking the peptidyl transferase activity of the 60S ribosomal subunit.
Anisomycin has been widely used as a research tool to study cellular processes dependent on protein synthesis, such as memory formation and neural plasticity.
Due to its potent biological effects, it is important for researchers to have access to reliable and reproducible protocols for working with anisomycin.
PubCompare.ai's AI-driven platform can help optimize anisomycin research by enabling easy access to protocols from literature, preprints, and patents, and providing intelligent analysis to identify the most robust and accurate methods.
This can enhance the quality and reproducibility of anisomycin studies, leading to more meaningful insights into its biological functions.

Most cited protocols related to «Anisomycin»

FRET Biosensor Assay in HeLa and Cos7 Cells

HeLa cells were purchased from the Human Science Research Resources Bank (Sennanshi, Japan). The Cos7 cells used were Cos7/E3, a subclone of Cos7 cells established by Y. Fukui (National Research Institute of Health, Taiwan, Republic of China). HeLa cells and Cos7 cells were maintained in DMEM (Sigma-Aldrich, St. Louis, MO) supplemented with 10% FBS. The cells were plated on 35-mm glass base dishes or 96-well glass base plates (Asahi Techno Glass, Tokyo, Japan), which were coated with collagen type I (Nitta Gelatin, Osaka, Japan). Plasmids encoding FRET biosensors were transfected into HeLa cells and Cos7 cells by 293fectin or Lipofectamine 2000, according to the manufacturer's instructions (Invitrogen, San Diego, CA), respectively. EGF was purchased from Sigma-Aldrich. dbcAMP, TPA, Calyculin A, Anisomycin, PD153035, and JNK inhibitor VIII were purchased from Calbiochem (La Jolla, CA). PD184352 was obtained from Toronto Research Chemicals (Ontario, Canada). BI-D1870 was purchased from Symansis (Shanghai, China). Rapamycin was obtained from LC Laboratories (Woburn, MA). PLX-4720 was purchased from Selleck Chemicals (Houston, TX). The expression vector of piggyBac transposase was provided by A. Bradley (Wellcome Trust Sanger Institute, Cambridge, UK; Yusa et al., 2009 (link)). Phos-tag was obtained from the Phos-tag Consortium (Hiroshima, Japan; www.phos-tag.com). Anti-green fluorescence protein (GFP) sera were prepared in our laboratory. LI-COR (Lincoln, NE) blocking buffer and the IRDye680- and IRDye800-conjugated anti–rabbit and anti–mouse immunoglobulin G secondary antibodies were obtained from LI-COR.

Komatsu N., Aoki K., Yamada M., Yukinaga H., Fujita Y., Kamioka Y, & Matsuda M. (2011). Development of an optimized backbone of FRET biosensors for kinases and GTPases. Molecular Biology of the Cell, 22(23), 4647-4656.

Publication 2011

1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-ol Anisomycin Anti-Antibodies BI D1870 Biosensors Bucladesine Buffers calyculin A Cells Cloning Vectors Collagen Type I Fluorescence Resonance Energy Transfer Gelatins Green Fluorescent Proteins HeLa Cells Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulin G IRDye800 lipofectamine 2000 Manpower Mus PD 153035 PD 184352 Plasmids PLX 4720 Rabbits Serum Sirolimus Transposase

Measuring Programmed Ribosomal Frameshifting

Escherichia coli strain DH5α was used to amplify plasmids, and E.coli transformations were performed using the high efficiency method of Inoue et al. (13 (link)). YPAD and synthetic complete medium (H-) were used as described previously (14 (link)). Yeast strain JD932 (MATa ade2-1 trp1-1 ura3-1 leu2-3,112 his3-11,15 can1-100) (15 (link)) was used for in vivo measurement of programmed −1 ribosomal frameshifting. Yeast cells were transformed using the alkali cation method (16 (link)). Dual luciferase plasmids pJD375 (C₁, no frameshift signal) and pJD376 (F₁, L-A virus gag-pol frameshift signal) have been described previously (7 (link)). Putative frameshift signals from S.cerevisiae genes YOR026W/BUB3 (F₂, plasmid pJD519) and YPL128C/TBF1 (F₃, plasmid pJD478) were constructed as follows: (i) oligonucleotides from Integrated DNA Technology (Coralville, IA) were annealed and gel purified, and (ii) annealing the oligonucleotides 5′-TCGACAAAAAATCATCTTTCAGGGTGGATTGGAACGGCCCCAGTGATCCTGAGAACCCACAAAACTGGCCCG-3′ to 5′-GATCCGGGCCAGTTTTGTGGGTTCTCAGGATCACTGGGGCCGTTCCAATCCACCCTGAAAGATGATTTTTTG-3′ (F₂), and 5′-CGACAAATTTATCTCAAGCATCCTTCATCAGCTGCATCTGCTACTGAAGAGGG-3′ to 5′-GATCCTCTTCTGTAGCAGATGCAGCTGAAGAAGGATGCTGAGATAAATTTG-3′ (F₃) left overhanging single-stranded DNA complementary to SalI and BamHI restriction sites. The annealed oligonucleotides were ligated into p2mci (6 (link)). The frameshift signal was sub-cloned as a SalI–EcoRI fragment into similarly digested pJD375. The open reading frame (ORF) 1a-1b frameshift signal from the SARS-associated Coronavirus (SARS-CoV) was cloned; sense 5′-GATCCTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTCTACAGGGCTTTTGAGCT-3′ and antisense 5′-CAAAAGCCCTGTAGACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAAG-3′ oligonucleotides were annealed, gel purified and cloned into BamHI and SacI restricted p2mc (6 (link)). This was further sub-cloned into a pJD375-based plasmid where the reading frame was corrected using site-directed mutagenesis to add a cytosine downstream of the BamHI restriction site (F₄). A zero-frame control (C₂) plasmid was made by inserting two cytosine residues upstream of the BamHI restriction site and cells were grown in the absence or presence of 20 μg/ml of anisomycin (Sigma–Aldrich, St. Louis, MO). The annealed oligonucleotides were ligated into p2mci (6 (link)). The SARS-CoV frameshift signal (F₄) was sub-cloned as a SalI–EcoRI fragment into similarly restricted pJD375. In vivo DLAs for programmed −1 ribosomal frameshifting were performed in yeast strain JD1158 as described previously (7 (link)). Luminescence readings were obtained using a Turner Designs TD20/20 luminometer (Sunnyvale, CA). Reactions were carried out using the Dual-Luciferase® Reporter Assay System from Promega Corporation (Madison, WI).

Jacobs J.L, & Dinman J.D. (2004). Systematic analysis of bicistronic reporter assay data. Nucleic Acids Research, 32(20), e160.

Publication 2004

Alkalies Anisomycin Antisense Oligonucleotides Biological Assay Cells Cytosine Deoxyribonuclease EcoRI DNA, Single-Stranded Escherichia coli Frameshift Mutation Genes Luciferases Luminescence Mutagenesis, Site-Directed Oligonucleotides Plasmids Promega Reading Frames Saccharomyces cerevisiae Severe acute respiratory syndrome-related coronavirus Strains tyrosinase-related protein-1 Virus

Quantitative Single-Cell Image Analysis

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Publication 2014

Anisomycin Cell Nucleus Cells Cytoplasm Microtubule-Associated Proteins Proteins Python

Hyperalgesic Priming Model in Rats for Pain Research

The experimental design was based on previous experiments demonstrating “hyperalgesic priming” in rats (K. O. Aley et al., 2000 (link); C. A. Parada et al., 2003 (link); C. A. Parada et al., 2005 (link); O. A. Dina et al., 2008 (link); D. B. Reichling and J. D. Levine, 2009 (link)). Animals were placed in acrylic boxes with wire mesh floors and baseline mechanical withdrawal thresholds of the left hindpaw were measured after habituation for 1 hr using the up-down method (S. R. Chaplan et al., 1994 (link)). The experimenter making measurements was always blinded to the experimental conditions. For day 1 experiments, IL-6 was injected into plantar surface of the left hindpaw in a volume of 25 μL. For paw co-injection experiments drugs were co-injected with IL-6 and IL-6 + nerve growth factor (NGF) at doses determined previously (O. K. Melemedjian et al., 2010 (link)). Plantar incision was done as described previously (R. K. Banik et al., 2006 (link)). For intrathecal (i.t.) treatments, drugs were injected immediately after intraplantar injections under brief (less than 3 min) isoflurane anesthesia in a volume of 5 μL (J. L. Hylden and G. L. Wilcox, 1980 (link)). Drug concentrations for temsirolimus (T. J. Price et al., 2007 (link)), ZIP (R. Shema et al., 2007 (link)), anisomycin (K. Nader et al., 2000 (link)) and pep2m (Y. Yao et al., 2008 (link)) were based on published findings and concentrations for 4EGI-1 were determined in pilot experiments. For experiments with i.t. treatments on day 4 or later, mice were tested prior to i.t. injection to assure that allodynia had completely resolved. I.t. injections were done at these time points under isoflurane anesthesia as described above. PGE₂ (100 ng) was injected on day 6 or 18 in the plantar surface of the left hindpaw in a volume of 25 μL. Allodynia testing was then done at the time points indicated in the text. For viral vector experiments, virus was injected i.t. diluted in saline to 10¹⁴ plaque forming units per mL. Transduction was measured 31 days after i.t. injection by visualizing mCherry in spinal cord slices (20 um thick) and Neuronal Nucleus marker (NeuN) labeled with donkey anti-mouse Alexa fluor 488 (Invitrogen) with a Zeiss LSM 510 confocal microscope.

Asiedu M.N., Tillu D.V., Melemedjian O.K., Shy A., Sanoja R., Bodell B., Ghosh S., Porreca F, & Price T.J. (2011). Spinal Protein Kinase M ζ Underlies the Maintenance Mechanism of Persistent Nociceptive Sensitization. The Journal of Neuroscience, 31(18), 6646-6653.

Publication 2011

alexa fluor 488 Allodynia Anesthesia Animals Anisomycin Cell Nucleus Cloning Vectors Dental Plaque Dinoprostone Equus asinus Hyperalgesia Investigational New Drugs Isoflurane Microscopy, Confocal Mus Nerve Growth Factor Neurons Pharmaceutical Preparations Rattus Saline Solution Spinal Cord temsirolimus Therapies, Investigational Virus

Puromycin Labeling for Localized Protein Synthesis

For puromycylation, neurons were incubated with (or in ‘no puro’ controls without) 1–3 μM puromycin for 2 – 15 min (as indicated) in full medium at 37 °C in a humidified atmosphere with 5 % CO₂. Incubation was stopped by two fast washes in prewarmed PBS-MC and cells were fixed for 20 min in PFA-sucrose. In protein synthesis inhibitor control experiments cells were pretreated with 40 μM anisomycin for 30 min before addition of puromycin to the medium. Experiments to determine site of synthesis were, in addition, carried out with a 30 min pretreatment with 355 μM cycloheximide which stalls the truncated protein at the ribosome and enhances puromycylation^{3 (link)}. After fixation, cells were washed, permeabilized and treated as described in the sections ‘PLA’ (using puromycin antibody and protein of interest antibody as primary antibody pair for 2 h) and ‘immunocytochemistry’.

Dieck S.T., Kochen L., Hanus C., Bartnik I., Nassim-Assir B., Merk K., Mosler T., Garg S., Bunse S., Tirrell D.A, & Schuman E.M. (2015). Direct visualization of identified and newly synthesized proteins in situ. Nature methods, 12(5), 411-414.

Publication 2015

Anabolism Anisomycin Atmosphere Cells Cycloheximide Immunocytochemistry Immunoglobulins Neurons Proteins Protein Synthesis Inhibitors Puromycin Ribosomal Proteins Sucrose

Most recents protocols related to «Anisomycin»

Luteolin and Estrogen Receptor Inhibition in HepG2 Cells

HepG2 cells (Cell Bank of Chinese Academy of Sciences) were suspended in DMEM (BI, Israel) containing 10% fetal bovine serum (FBS; Gibco) and incubated at 37°C with 5% CO₂. To evaluate the effect of luteolin on HepG2 cells, 5, 10, 20, 40, 80, 160, and 320 μmol/L luteolin (Sigma) was used. To determine the effect of ESR1 inhibition on HepG2 cells, 1, 20, 50, 100, 300, 500, and 900 nmol/L fulvestrant (Selleck China) was used. To determine the effect of Akt and MAPK-JNK signaling on ESR1 expression, Akt agonist SC79 (10 μmol/L, Selleck China), and MAPK-JNK agonist anisomycin (4 μmol/L, Selleck China) were used. DMSO served as a control.

Yu Y., Ding S., Xu X., Yan D., Fan Y., Ruan B., Zhang X., Zheng L., Jie W, & Zheng S. (2023). Integrating Network Pharmacology and Bioinformatics to Explore the Effects of Dangshen (Codonopsis pilosula) Against Hepatocellular Carcinoma: Validation Based on the Active Compound Luteolin. Drug Design, Development and Therapy, 17, 659-673.

Publication 2023

Anisomycin Cells Chinese Fulvestrant Hep G2 Cells Luteolin Psychological Inhibition Sulfoxide, Dimethyl

Imaging SH-SY5Y cells with FRET biosensor

SH-SY5Y cells were plated in a 35-mm glass-bottom dish at a density of 25,0000 cells/ml. Cells were transiently transfected with 1.5 μg of pcDNA JNKAR1 using jetPRIME transfection reagent. Following transfection, cells were incubated for 16 hours under standard tissue culture conditions and then stimulated with 300 nM anisomycin. Images were taken at 5-min intervals over 1 hour with a Leica TCS SP8 MP using the 40× 1.20 numerical aperture (NA) water immersion lens with a 481/32 filter cube. The donor fluorophore, cerulean, was excited with a wavelength of 840 nm, and the fluorescence lifetime was quantified with FLIMfit 5.1.1 software (53 (link)) rendered using ImageJ [National Institutes of Health (NIH)] and plotted with GraphPad Prism (version 9).

Hastings J.F., Latham S.L., Kamili A., Wheatley M.S., Han J.Z., Wong-Erasmus M., Phimmachanh M., Nobis M., Pantarelli C., Cadell A.L., O’Donnell Y.E., Leong K.H., Lynn S., Geng F.S., Cui L., Yan S., Achinger-Kawecka J., Stirzaker C., Norris M.D., Haber M., Trahair T.N., Speleman F., De Preter K., Cowley M.J., Bogdanovic O., Timpson P., Cox T.R., Kolch W., Fletcher J.I., Fey D, & Croucher D.R. (2023). Memory of stochastic single-cell apoptotic signaling promotes chemoresistance in neuroblastoma. Science Advances, 9(9), eabp8314.

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Publication 2023

Anisomycin Cells Fluorescence Hyperostosis, Diffuse Idiopathic Skeletal Lens, Crystalline prisma Submersion Tissue Donors Tissues Transfection

NAFLD and NASH Mouse Models

Male and female C57BL/6 mice (8 weeks old) were purchased from Weitong Lihua Experimental Animal Technology Co., Ltd (Beijing, China) and kept in specific pathogen-free animal house. The room temperature was controlled at around 23 °C and humidity was kept at 50–60% with a 12 h day/night cycle. The water was sufficient to obtain and in order to eliminate the potential appetite suppression role of tamoxifen, the mice with tamoxifen administration and their control mice were pair-fed unless specifically stated. In order to establish NASH mouse model, male or female mice were fed with a MCD diet (A02082002BR, Research Diets, New Brunswick, USA) for 6 weeks or a CDAA diet (A06071309, Research Diets, New Brunswick, USA) for 10 weeks. To establish a NAFLD mouse model, male or female mice were fed with a HFD (D12492, Research Diets, New Brunswick, USA) for 20 weeks. Tamoxifen was purchased from Sigma (T5648, USA), dissolved in corn oil, and stored at 4 °C for at most a week. Pharmacological ER antagonist fulvestrant (S1191) and JNK activator anisomycin (S7409) were purchased from Selleck (Shanghai, China). The dosing scheme was shown in figures and illustrated in the Results section.
All animal experiments were approved by the Animal Experiment Administration Committee of the Fourth Military Medical University (Xi’an, China) and proceeded under the instruction of Guide for the Care and Use of Laboratory Animals published by the National Institute of Health (publications 86-23, revised 1985).

Fang Z., Xu H., Duan J., Ruan B., Liu J., Song P., Ding J., Xu C., Li Z., Dou K, & Wang L. (2023). Short-term tamoxifen administration improves hepatic steatosis and glucose intolerance through JNK/MAPK in mice. Signal Transduction and Targeted Therapy, 8, 94.

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Publication 2023

Animals Animals, Laboratory Anisomycin CDAA Corn oil Diet Females Fulvestrant Humidity Males Mice, House Mice, Inbred C57BL Military Personnel Non-alcoholic Fatty Liver Disease Nonalcoholic Steatohepatitis Specific Pathogen Free Tamoxifen

Lipotoxicity and Tamoxifen Assay in Cell Lines

Cell lines were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). Huh7 and HepG2 cell lines were grown in RPMI1640 or high-glucose DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 100 U/ml penicillin and 0.1 mg/ml streptomycin (MI00614, Mishushengwu, Xi’an, China). AML12 cell line was grown in DMEM: F12 medium (11330, Invitrogen, USA) supplemented with 10% FBS, 1% ITS liquid media supplement (I3146, Sigma, USA), and 40 ng/ml dexamethasone. All cell lines were kept in a humidified incubator at 37 °C and 5% CO₂. Cells were used from third to tenth passage in each experiment. Primary hepatocytes were isolated from 8-week-old male C57BL/6 mice and were cultured in HM medium (ScienCell, USA) and seeded in six-well plates at 1 × 10⁶/well. To induce cellular lipotoxicity, 0.3 mM sodium palmitate (SYSJ-KJ, Kunchuang Biotechnology, Xi’an, China) was added into medium and vehicle was added as a control. After 36 h, tamoxifen dissolved in DMSO was added at a dose of 10, 20, 40 μM and kept for 36 h and DMSO was added as a control. To induce JNK activation in vitro, anisomycin (10 μM) was added 2 h before tamoxifen treatment. To inhibit JNK phosphorylation in vitro, 30 μM Tanzisertib (cc-930) (S8490, Selleck, China) was added 2 h before tamoxifen treatment. For cellular Oil Red O (ORO) staining, cells were cultured in six-well plates and after sodium palmitate and tamoxifen treatment, culture medium was removed, and cells were fixed with 4% paraformaldehyde for 30 min and washed with PBS three times. Then the cells were treated with 60% isopropanol for 5 min. Remove isopropanol, stain cells with ORO working solution (Servicebio Technology, Wuhan, China) for 10 min, and washed cells with PBS. Then the cells were stained with hematoxylin for 3–5 min and washed with PBS at least three times. Then we observed and took photos using an inverted phase contrast microscope (Olympus, X71, Japan). For cellular TC and TG tests, we purchased commercial kits from Pulilai Gene Technology Co., Ltd (Beijing, China) and followed the manufacturer’s instructions.

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Publication 2023

4-(9-(tetrahydrofuran-3-yl)-8-(2,4,6-trifluorophenylamino)-9H-purin-2-ylamino)cyclohexanol Anisomycin Cardiac Arrest CC-930 Cell Lines Cells Chinese Culture Media Dexamethasone Dietary Supplements Fetal Bovine Serum Genes Glucose Hepatocyte Hep G2 Cells Isopropyl Alcohol Males Mice, Inbred C57BL Microscopy, Phase-Contrast paraform Penicillins Phosphorylation Sodium Palmitate solvent red 27 Stains Streptomycin Sulfoxide, Dimethyl Tamoxifen

Antibody Sources and Expression Vectors

Anisomycin, the polyclonal rabbit anti-B4GALNT2 antibody (HPA015721), the monoclonal mouse anti-GFP (G1546), the monoclonal mouse anti-Flag M2 (F3165), the polyclonal rabbit anti-TMEM165, and the mouse anti-β-actin antibodies were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Anti-Sd^a (KM694) antibody was a kind gift from Dr. Taeko Dohi and Dr. Akiko Furuya (Biologics Research Laboratories, Research Division, Kyowa Hakko Kirin Co., Tokyo, Japan). The polyclonal goat anti-mouse horseradish peroxidase-conjugated IgG was purchased from Agilent Technologies (Santa Clara, CA, USA). The polyclonal goat anti-mouse horseradish peroxidase-conjugated IgM was purchased from Fisher Scientific (Illkirch, France). The polyclonal goat anti-rabbit horseradish peroxidase-conjugated IgG was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). The secondary antibodies Alexa fluor^® 488 anti-rabbit and Alexa fluor^® 568 anti-rabbit were from Life Technologies (Fisher Scientific, Illkirch Graffenstaden, France). The polyclonal rabbit anti-Calnexin was purchased from Enzo life technologies (Villeurbanne, France). The GFP expression vector pFx was a kind gift of Dr. Jack Röhrer (University of Zurich), the expression vectors pEGFP-N1 and pmCherry-N1 were from Clontech, whereas p3×FLAG-CMVTM-10 was from Sigma.

Cogez V., Vicogne D., Schulz C., Portier L., Venturi G., de Ruyck J., Decloquement M., Lensink M.F., Brysbaert G., Dall’Olio F., Groux-Degroote S, & Harduin-Lepers A. (2023). N-Glycan on the Non-Consensus N-X-C Glycosylation Site Impacts Activity, Stability, and Localization of the Sda Synthase B4GALNT2. International Journal of Molecular Sciences, 24(4), 4139.

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Publication 2023

Actins alexa 568 alexa fluor 488 Anisomycin Anti-Antibodies anti-IgG anti-IgM Antibodies Antibodies, Anti-Idiotypic Biological Factors Calnexin Cloning Vectors Goat Horseradish Peroxidase Immunoglobulins Mus Rabbits

Top products related to «Anisomycin»

Anisomycin by Merck Group

Sourced in United States, Germany, Sao Tome and Principe, France, United Kingdom, Israel

Anisomycin is a laboratory reagent used in biochemical and cell biology research. It is a protein synthesis inhibitor that acts by blocking the peptidyl transferase activity of the 60S ribosomal subunit, thereby preventing the formation of peptide bonds during translation. Anisomycin is commonly used as a tool to study cellular signaling pathways and gene expression regulation.

Cycloheximide (chx) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium

Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Anisomycin by Bio-Techne

Sourced in United Kingdom

Anisomycin is a laboratory product manufactured by Bio-Techne. It is a protein synthesis inhibitor, which functions by blocking the peptidyl transferase activity of the 60S ribosomal subunit, thereby inhibiting translation elongation.

Anisomycin by Selleck Chemicals

Sourced in United States, China

Anisomycin is a laboratory reagent used for scientific research purposes. It is a protein synthesis inhibitor, which means it has the ability to block the process of protein production in cells. Anisomycin is commonly used in cell and molecular biology studies to investigate cellular mechanisms and signaling pathways.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Sp600125 by Merck Group

Sourced in United States, Germany, United Kingdom, Macao, China, Sao Tome and Principe, Morocco, Sweden, Japan, Switzerland, France, India, Italy, Canada, Brazil

SP600125 is a small molecule compound that functions as a selective inhibitor of the c-Jun N-terminal kinase (JNK) signaling pathway. It is commonly used as a research tool to investigate the role of the JNK pathway in various cellular processes and disease models.

Puromycin by Merck Group

Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, Macao, Canada, Japan, Italy, Switzerland, France, Israel, Australia, Spain, Belgium, Morocco, Sweden

Puromycin is a laboratory product manufactured by Merck Group. It functions as an antibiotic that inhibits protein synthesis in eukaryotic cells.

Anisomycin by Cayman Chemical

Sourced in United States

Anisomycin is a laboratory reagent used for research purposes. It is a natural product isolated from certain Streptomyces bacterial species. Anisomycin functions as a protein synthesis inhibitor, preventing the formation of new proteins in cells.

Anisomycin by MedChemExpress

Sourced in United States

Anisomycin is a natural product that functions as a protein synthesis inhibitor. It acts by binding to the 60S ribosomal subunit, preventing the formation of the 80S initiation complex and thus inhibiting translation.

What is Anisomycin and what are its key properties?

Anisomycin is a natural product isolated from the bacterium Streptomyces griseolus. It is a potent protein synthesis inhibitor that works by blocking the peptidyl transferase activity of the 60S ribosomal subunit. Anisomycin has been widely used as a research tool to study cellular processes dependent on protein synthesis, such as memory formation and neural plasticity.

What are the main challenges researchers face when working with Anisomycin?

How can PubCompare.ai help optimize Anisomycin research?

PubCompare.ai's AI-driven platform can help researchers optimize their Anisomycin studies by enabling easy access to protocols from literature, preprints, and patents, and providing intelligent analysis to identify the most robust and accurate methods. This can enhance the quality and reproducibility of Anisomycin studies, leading to more meaningful insights into its biological functions. The platform allows you to screen protocol literature more efficiently, and leverage AI to pinpoint critical insights that help you choose the best protocols for your specific research goals.

What are some common applications of Anisomycin?

Are there different types or variations of Anisomycin?

More about "Anisomycin"

Anisomycin is a natural product derived from the bacterium Streptomyces griseolus.
It is a potent protein synthesis inhibitor, blocking the peptidyl transferase activity of the 60S ribosomal subunit.
This natural antibiotic has been extensively used as a research tool to investigate cellular processes that rely on protein synthesis, such as memory formation and neural plasticity.
Researchers often utilize Anisomycin alongside other compounds like Cycloheximide, another protein synthesis inhibitor, or DMSO, a common solvent, to study its effects on various biological systems.
The addition of fetal bovine serum (FBS) to cell culture media can also impact Anisomycin's activity.
Additionally, the c-Jun N-terminal kinase (JNK) inhibitor SP600125 has been employed in conjunction with Anisomycin to dissect signaling pathways.
To ensure the quality and reproducibility of Anisomycin studies, it is crucial for researchers to have access to reliable and optimized protocols.
PubCompare.ai's AI-driven platform can assist in this process by providing easy access to Anisomycin-related protocols from literature, preprints, and patents, and offering intelligent analysis to identify the most robust and accurate methods.
This can lead to enhanced quality and reproducibility in Anisomycin research, ultimately enabling more meaningful insights into its biological functions.
Additionally, Puromycin, another protein synthesis inhibitor, may be used in combination with Anisomycin to further investigate cellular processes and protein synthesis dynamics.
By leveraging the power of PubCompare.ai, researchers can streamline their Anisomycin-related studies and maximize the impact of their findings.