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Nystatin

Nystatin is a polyene antifungal medication used to treat various fungal infections, including candidiasis, oral thrush, and intestinal candidiasis.
It works by disrupting the cell membrane of fungal cells, leading to their death.
Nystatin is typically applied topically or taken orally, and is considered a safe and effective treatment option for many fungal infections.
However, it is important to follow the instructions of healthcare professionals when using nystatin, as improper use can lead to side effects such as skin irritation or gastrointestinal discomfort.
Overall, nystatin remains a valuable tool in the management of fungal infections, with its efficacy and safety profile making it a commonly prexcribed antifungal medication.

Most cited protocols related to «Nystatin»

Patch-clamp recordings were carried out under an Olympus microscope (BX51WI) with an EPC-10 amplifier and the Pulse software (HEKA) using a protocol modified from previous studies43 (link),44 (link). In brief, worms were glued to a sylgard-coated coverglass covered with bath solution and a small piece of cuticle in the worm head was cut open and pinned down to the coverglass to expose the cells. The ASJ neuron was identified by an mCherry fluorescence marker expressed as a transgene driven by the trx-1 promoter. mCherry was excited by orange light (590 ± 10 nm). Background light was filtered into red with a red filter. Light pulses (0.5 s) were delivered from an Arc lamp (EXFO Xcite) coupled to a mechanical shutter (Sutter) triggered by the amplifier. Recording pipettes were pulled from borosilicate glass and fire-polished. The bath solution contains 145 mM NaCl, 5 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 11 mM dextrose and 5 mM HEPES (330 mOsm, pH adjusted to 7.3). The pipette solution for perforated patch clamp contained 115 mM potassium gluconate, 15 mM KCl, 5 mM MgCl2, 10 mM HEPES, 0.25 mM CaCl2, 20 mM sucrose, 5 mM EGTA and 50 µg ml−1 nystatin (315 mOsm, pH adjusted to 7.2). We included 5 mM Na2ATP and 0.5 mM Na2GTP in the pipette solution during classic whole-cell recording. When acquiring voltage-ramp traces, potassium gluconate was replaced with CsCl in the pipette solution. Nystatin was included in the pipette solution only during perforated whole-cell recording. Several other ionophores were also tested for perforated patch clamp (for example, β-escin, amphotericin B and gramicidin), and nystatin was found to be the most efficient under our conditions. Voltages were clamped at −70 mV. Current data were sampled at 5 kHz. Series resistance and membrane capacitance were both compensated for during recording.
Publication 2008
Amphotericin B Bath Cells cesium chloride Egtazic Acid Escin Fluorescence gluconate Glucose Gramicidin Head Helminths HEPES Ionophores Light Magnesium Chloride Microscopy Neurons Nystatin Patch Tests Potassium Pulse Rate Pulses Sodium Chloride Sucrose Tissue, Membrane Transgenes TXN protein, human
High titer virus stocks of paramyxoviruses used for validation of broad reactivity of the assay are listed in table 1. Clinical specimens from humans to test for sensitivity of fragment analysis were obtained from the clinical diagnostic unit of the virology department, and were anonymized. Wild birds were trapped by expert ornithologists. Cloacal and/or oropharyngeal swab specimens were collected with sterile cotton swabs. All samples were stored in transport medium consisting of Hanks balanced salt solution containing 0.5% lactalbumin, 10% glycerol, 200 U/mL penicillin, 200 µg/mL streptomycin, 100 U/mL polymyxin B sulfate, 250 µg/mL gentamicin, and 50 U/mL nystatin (ICN, Zoetermeer, The Netherlands). All bird samples were stored at −80°C or at −20°C if rapid transport or storage at −80°C was not possible. Frozen samples were stored at −80°C in the laboratory upon arrival and were thawed no more than two times prior to analysis.
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Publication 2012
Aves Biological Assay Diagnosis Enterobacter Freezing Gentamicin Glycerin Gossypium Hanks Balanced Salt Solution Homo sapiens Hypersensitivity Lactalbumin Nystatin Oropharynxs Penicillins Polymyxin B Sulfate Sterility, Reproductive Streptomycin
The modified agar well diffusion method of Perez et al., [27 ] was employed. Each selective medium was inoculated with the microorganism suspended in SBCB. Once the agar was solidified, it was punched with a six millimeters diameter wells and filled with 25 μL of the plants extracts and blanks (ethanol, distilled water, and hexane). The concentration of the extracts employed was 25 μg/ml. Simultaneously, gentamycin sulfate (S. aureus, P. aeruginosa, E. coli, and B. cereus), clindamycin (S. β hemolytic), and nystatin (C. albicans) were used as positive controls at a concentration of 1.0, 0.3 and 1.0 μg/ml respectively. The dilution medium for the positive controls was sterile distilled water. The test was carried out by triplicate. The plaques were incubated at 35 ± 2°C for 24 h, except for C. albicans which was incubated at 29 ± 2°C. The antimicrobial activity was calculated by applying the expression:
%RIZD=(IZD sampleIZD negative control)IZD antibiotic standard×100%     (1) MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=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@826A@
Where RIZD is the percentage of relative inhibition zone diameter and IZD is the inhibition zone diameter (mm). Equation (1) compensates the possible effect of the solvent (blank) other than water on the IZD. The resulting IZD of the samples were either higher than or equal to the IZD of the blanks. Therefore, the obtained percentages were positive (Table 1). '[see Additional file table1]'. The test was considered negative (-) when the IZD of the sample was equal to the IZD of the blank.
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Publication 2006
Agar Antibiotics Candida albicans Clindamycin Diffusion Escherichia coli Ethanol Hemolysis Microbicides n-hexane Nystatin Plants Pseudomonas aeruginosa Psychological Inhibition Senile Plaques Solvents Staphylococcus aureus Sterility, Reproductive Sulfate, Gentamicin Technique, Dilution
Helicobacter pylori strains were grown on GC agar plates (Difco) supplemented with vitamin mix (1%), horse serum (8%), vancomycin (10 mg/l), trimethoprim (5 mg/l), and nystatin (1 mg/l) (serum plates), and incubated for 16–60 h in a microaerobic atmosphere (85% N2, 10% CO2, 5% O2) at 37°C. Escherichia coli strains were grown on Luria–Bertani (LB) agar plates supplemented with ampicillin (100 mg/l), chloramphenicol (30 mg/l), or kanamycin (40 mg/l), as appropriate.
Publication 2010
Agar Ampicillin Atmosphere Chloramphenicol Equus caballus Escherichia coli Helicobacter pylori Kanamycin Nystatin Serum Strains Trimethoprim Vancomycin Vitamins
We placed each captured duck in a box with a clean (unused) paper bottom. Using sterile cotton swabs, we then sampled each bird either by swirling the swab in its cloaca (20% of individual birds) or by swabbing its fresh droppings on the paper bottom. Cotton swabs were immediately put in vials containing virus transport media (Hanks balanced salt solution containing 0.5% lactalbumin, 10% glycerol, 200 U/mL penicillin, 200 μg/mL streptomycin, 100 U/mL polymyxin B sulfate, 250 μg/mL gentamicin, and 50 U/mL nystatin (ICN, Zoetermeer, the Netherlands)) and frozen to –70°C within 30 min.
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Publication 2007
Aves Ducks Enterobacter Freezing Gentamicin Glycerin Gossypium Hanks Balanced Salt Solution Lactalbumin Nystatin Penicillins Polymyxin B Sulfate Sterility, Reproductive Streptomycin Virus

Most recents protocols related to «Nystatin»

Primary cells of the second trimester were purchased (Cat number 7120, ScienCell, Carlsbad, CA). As demonstrated in our previous study, EVs exert different effects on early stage trophoblast vs. term trophoblast cell cultures. Specifically, in HP women, treatment with EVs decreased apoptosis and induced higher migration in early-stage trophoblasts compared with untreated. Conversely, exposure to EVs obtained from women with GVCs increased term trophoblast apoptosis and inhibited early-stage trophoblast migration when compared to cells exposed to HP EVs (Shomer et al., 2013 (link)). Because GVCs begin earliest at 20 weeks of gestation (Peracoli et al., 2019 (link)), we preferred to study the effects of EVs on early stage trophoblasts and therefore did not culture HVT from term placentas. HVT were grown in a recommended trophoblast medium (50%, catalog number 7121, Sciencell) supplemented with Dulbecco’s Modified Eagle Medium–high glucose (22%, Biological Industries, Israel), F-12 (HAM) nutrient mixture (22%, Biological Industries, Israel), fetal calf serum (FCS) (10%, Biological Industries, Israel), and penicillin G sodium salt (10,000 units/mL) - streptomycin sulfate (10 mg/ml)–nystatin (1,250 units/mL) solution (1%, Biological Industries, Israel). The cells were grown at 37°C, 5% CO2. Passages 4-8 were used for experiments. HVT cell cultures were labeled with anti-hPL to ensure quality control of their contents. As in our previous studies, greater than 90% labeling with anti-hPL was considered a pure trophoblast culture (Shomer et al., 2013 (link)).
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Publication 2023
Apoptosis Biopharmaceuticals Cell Culture Techniques Cells Eagle Fetal Bovine Serum Glucose Nutrients Nystatin Penicillin G Sodium Placenta Pregnancy Streptomycin Sulfate Trophoblast Woman
Throat swabs from the oropharyngeal area and from one tonsil were performed by experienced health care professionals using flocked plastic swabs (ESwab, Copan Diagnostics, Murrieta, CA, USA). Samples were placed in the ESwab tubes containing Amies transport media (Copan Diagnostics), and transported within 8 h to the national reference laboratory for N. meningitidis at Örebro University Hospital, where a duplex PCR targeting ctrA and crgA [23 (link)] was performed within 24 h. If found to be positive for both or either of ctrA or crgA, samples were subsequently cultured on selective and non-selective agar plates (GC agar with added vancomycin, colistin, nystatin and trimethoprim; and plain GC agar, respectively) incubated for 24 h in a humid CO2-enriched (5%) atmosphere at 36 ± 1 °C. If no visible growth was noticed after 24 h, the plates were incubated for an additional 24 h. N. meningitidis was confirmed by colony appearance, oxidase positivity and MALDI-TOF mass spectrometry (Bruker Daltonik GmbH, Bremen, Germany) and then subcultured and preserved.
The ctrA/crgA PCR used in this study was originally developed to detect invasive meningococcal isolates from fluids normally considered sterile. As a confirmatory analysis of the ctrA/crgA PCR, all collected samples were additionally analysed with a second PCR targeting sodC and porA, adapted from a previous carriage study [24 (link)]. Samples were considered as true positive if the culture showed growth of N. meningitidis, or showed positivity in both the ctrA/crgA and sodC/porA duplex PCRs (but not necessarily for all four PCR targets).
Except for the first sample occasion, an additional throat swab was performed that was placed in a DNA/RNA Shield Collection Tube (Zymo Research, Irvine, CA, USA) and stored for future RNA analyses.
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Publication 2023
Agar Atmosphere Colistin Diagnosis Health Care Professionals Mass Spectrometry Meningococcal Polysaccharide Vaccine Neisseria meningitidis Nystatin Oropharynxs Oxidases Palatine Tonsil Pharynx Specimen Collection Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sterility, Reproductive Trimethoprim Vancomycin

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Publication 2023
Agar Bacteria, Aerobic Biological Assay Candida albicans Cells Clotrimazole Ethanol Hartnup Disease Industrial Fungicides Minimum Inhibitory Concentration Nystatin Pharmaceutical Preparations Sulfoxide, Dimethyl Technique, Dilution Vision

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Publication 2023
Anti-Bacterial Agents Antifungal Agents Aspergillus niger Bacillus subtilis Bacteria Diffusion Escherichia coli Nystatin Psychological Inhibition Sulfoxide, Dimethyl Tetracycline
SARS-CoV-2 Spike proteins [recombinant SARS-CoV-2 Spike Protein (SP-RBD, Arg319-Phe 541; cat# RP-87678, HEK293 cell expressed and binds ACE2] was obtained from Life Technologies Corporation, Carlsbad, CA, USA. Mutants SPs and its control wild type protein were obtained from RayBiotech Inc., Peachtree Corners, GA, USA. Recombinant mutants N501Y (cat# 230-30184, expressed region Arg319-Phe541), D614G (Cat# 230-3030186, expressed region Arg319-Gln690), E484K (cat# 230-30188, expressed region Arg319-Phe541) and their control wild type SP (Cat# 230-30162, expressed region Arg319-Phe541) were also HEK 293 expressed. All SPs were labeled separately with Alexa Fluor 555, using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions. Anti-ACE2 antibody (R&D Systems, Cat# AF933) was labeled with Alexa Fluor 488 by following the manufacturer instructions (Microscale protein labeling kit; ThermoFisher Scientific). In addition, the labeled SPs or anti-ACE2 antibody were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate.
Antibodies raised against the extracellular domain of potential SP binding receptors were used. These include, anti-ACE2 antibody (R&D Systems, Cat# AF933) used at a low (10 μg/ml) and high (60 μg/ml) concentration; anti-TMPRSSE2 antibody (Invitrogen, Cat# PA5-14264) used at 13 μg/ml; anti-CD147 antibody (Invitrogen, Cat# 34-5600) used at 2.5 μg/ml; anti-NP-1 antibody (Invitrogen, Cat# PA5-47027) used at 2 μg/ml and anti-GM1 antibody (Abcam, Cat# Ab23943) used at 5 μg/ml. The concentration used was obtained from the manufacturer guidance. Wheat Germ agglutinin (WGA; Cat# L9640) and heparin (cat# H3393) were obtained from Sigma, and used at 10 and 100 μg/mL, respectively. Transferrin from human serum conjugated to Alexa Fluor 488 (Cat# T13342), BODIPY FL C5-Lactosylceramide complex to BSA (Cat# B34402) and BODIPY FL ganglioside (Cat# B13950) were obtained from ThermoFisher Scientific) and used at 10 μg/ml. Nystatin (Cat# J62486.09), and chlorpromazine (Cat# J63659) were obtained from ThermoFisher Scientific, and used at 25 and 10 μg/ml, respectively. Angiotensin II (cat# 1158/5) was obtained from R&D Systems and used at 0.1 μg/mL.
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Publication 2023
ACE2 protein, human alexa fluor 488 Alexa Fluor 555 Angiotensin II Antibodies Antibodies, Anti-Idiotypic BODIPY BSG protein, human CDw17 antigen Chlorpromazine Gangliosides HEK293 Cells Heparin Homo sapiens Nystatin Proteins Serum spike protein, SARS-CoV-2 Strains Transferrin Ultrafiltration Wheat Germ Agglutinins

Top products related to «Nystatin»

Sourced in United States, Germany, United Kingdom, Japan, Sao Tome and Principe, Brazil, Spain, Ireland, Slovenia, Israel, India, Australia, China, Poland
Nystatin is an antifungal medication used in the laboratory setting. It is a polyene macrolide antibiotic that functions by disrupting the cell membrane of fungal cells, leading to their death. Nystatin is commonly used for the treatment and prevention of fungal infections in in vitro experiments and laboratory procedures.
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Chlorpromazine is a pharmaceutical compound used as a laboratory reagent. It is a white crystalline solid that is soluble in water and organic solvents. Chlorpromazine is commonly used in research and laboratory settings as a reference standard or for various analytical purposes.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in Israel
Nystatin is a polyene antifungal medication used in the treatment of fungal infections. It is a naturally occurring compound produced by the bacterium Streptomyces noursei. Nystatin acts by binding to ergosterol, a component of the fungal cell membrane, which disrupts the membrane's integrity and leads to cell death.
Sourced in United Kingdom, United States
Nystatin is a polyene antifungal medication used for the treatment of fungal infections. It is available in various formulations, including topical creams, ointments, and oral suspensions. Nystatin functions by disrupting the cell membrane of fungal cells, leading to their death or inhibition of growth.
Sourced in United States, Germany, Sao Tome and Principe, United Kingdom, Israel
Dynasore is a small molecule compound used in laboratory research. It functions as a dynamin inhibitor, a protein involved in processes such as endocytosis. The core function of Dynasore is to inhibit the activity of dynamin, which is important for various cellular processes.
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Penicillin is a type of lab equipment used for the cultivation and production of penicillin, a widely used antibiotic. It is designed to provide a controlled environment for the growth and fermentation of the Penicillium fungus, which is the source of penicillin. The core function of Penicillin is to facilitate the efficient and reliable production of this important pharmaceutical compound.
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Streptomycin is a bacteriostatic antibiotic used in laboratory settings. It inhibits protein synthesis in bacterial cells. The core function of Streptomycin is to selectively target and disrupt the growth of certain bacterial species.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

More about "Nystatin"

Nystatin is a versatile polyene antifungal medication widely used to treat a variety of fungal infections, including candidiasis, oral thrush, and intestinal candidiasis.
This potent antifungal agent works by disrupting the cell membrane of fungal cells, leading to their demise.
Nystatin is typically administered topically or orally, and is considered a safe and effective treatment option for many fungal ailments.
Nystatin is often prescribed in conjunction with other medications, such as the antipsychotic Chlorpromazine, the solvent DMSO, the cell culture supplement FBS, and the endocytosis inhibitor Dynasore.
Additionally, it may be used alongside antibiotics like Penicillin and Streptomycin to combat fungal overgrowth.
When using Nystatin, it is crucial to follow the instructions of healthcare professionals to ensure proper usage and minimize the risk of side effects, such as skin irritation or gastrointestinal discomfort.
Nystatin's efficacy and safety profile make it a commonly prescribed antifungal medication, making it a valuable tool in the management of fungal infections.
Researchers can optimize their Nystatin studies by utilizing the AI-driven protocol comparisons offered by PubCompare.ai.
This innovative platform helps locate the best protocols from scientific literature, preprints, and patents, enhancing reproducibility and accuracy.
By exploring the power of PubCompare.ai, researchers can take their Nystatin research to new heights and unlock the full potential of this versatile antifungal agent.