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Penicillin V

Penicillin V is a semisynthetic penicillin antibiotic derived from the Penicillum fungus.
It is commonly used to treat bacterial infections, including streptococcal pharyngitis, pneumonia, and skin and soft tissue infections.
Penicillin V works by inhibiting cell wall synthesis in susceptible bacteria, leading to cell lysis and death.
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Most cited protocols related to «Penicillin V»

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Publication 2013
Acclimatization Acids Bone Matrix Bones Bos taurus Cancellous Bone Cells Chloroform Cytoplasmic Granules Edetic Acid Enzymes Freezing Lipids Methanol Penicillin V Powder Streptomycin Trypsin Vacuum
iCell Cardiomyocytes were plated and maintained in tissue culture-treated 384-well plates according to instructions provided by Cellular Dynamics International. Microplates were prepared by adding 25 μL of a 0.1% (w/v) gelatin solution per well and subsequent incubation for 2 h at 37°C and 5% CO2. Vials containing iCell Cardiomyocytes were removed from vapor phase liquid nitrogen storage immediately before plating and thawed for 4 min in a water bath at 37°C. The contents of a single vial were then resuspended in a total of 10 mL plating medium containing 1:500 (v/v) penicillin/streptomycin solution at room temperature. Cell density of the suspension was routinely tested by microscopic analysis of trypan blue-stained cells using disposable hemocytometers. Based on the determined cell density, the cell suspension was diluted in plating medium to provide a final cell concentration of 2 × 105 viable cells/mL. Subsequently, the gelatin solution was aspirated from the microplate and 25 μL of cell suspension was added per well, resulting in an estimated cell density of 5,000 viable cells/well. Plates were kept at room temperature for ∼30 min before they were incubated at 37°C and 5% CO2. Forty-eight hours following cell seeding, the plating medium was exchanged with 30 μL of maintenance medium containing 1:500 penicillin/streptomycin. Maintenance medium was subsequently changed every other day for 12 days. On the evening before an experiment, the medium was exchanged and replaced by 25 μL of fresh medium.
Publication 2015
Bath Cells Gelatins Microscopy Myocytes, Cardiac Nitrogen Penicillins Penicillin V Streptomycin Tissues Trypan Blue
ReNcell VM human neural precursor (ReN) cells were purchased from EMD Millipore (Billerica, MA, USA). The cells were plated onto BD Matrigel (BD Biosciences, San Jose, CA, USA)-coated T25 cell culture flask (BD Biosciences, San Jose, CA, USA) and maintained in DMEM/F12 (Life Technologies, Grand Island, NY, USA) media supplemented with 2 μg/ml Heparin (Stemcell Technologies, Vancouver, Canada), 2% (v/v) B27 neural supplement (Life Technologies, Grand Island, NY, USA), 20 μg/ml EGF (Sigma-Aldrich, St. Louis, MO, USA), 20 μg/ml bFGF (Stemgent, Cambridge, MA, USA), and 1% (v/v) Penicillin/Streptomycin/Amphotericin b solution (Lonza, Hopkinton, MA, USA) in CO2 cell culture incubator. The cell culture media were changed every 3 days until the cells were confluent. For 2D neuronal/glial differentiation, the cells were plated onto either Matrigel-coated 24-well or 6-well plates with DMEM/F12 differentiation media supplemented with 2 μg/ml Heparin, 2% (v/v) B27 neural supplement, and 1% (v/v) Penicillin/Streptomycin/Amphotericin b solution without growth factors. A half volume of the differentiation media was changed every 3 days for 3–7 weeks. DAPT, Compound E and BACE inhibitor IV were purchased from EMD Millipore, N-Lauroylsarcosine (Sarkosyl) from Sigma-Aldrich. Hematoxylin QS from Vector Laboratories (Burlingame, CA, USA), and Amylo-Glo from Biosensis (Thebarton, Australia). SGSM41 is an aminothiazole-bridged heterocycle-containing soluble γ-secretase modulator (SGSM) similar in structure to those published recently19 (link). SGSM 41 has the typical characteristics of this series of SGSM molecules that potently inhibit the production of Aβ42 and to a lesser degree Aβ40 while concomitantly increasing the generation of shorter Aβ peptide species such as Aβ38 and Aβ37. The structures and the detailed properties are included in Extended Data Table 1.
Publication 2014
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol Amphotericin Amphotericin B Cardiac Arrest Cell Culture Techniques Cells Cloning Vectors Cortisone Culture Media Dietary Supplements Fifth Cranial Nerves Growth Factor Hematoxylin Heparin Homo sapiens matrigel N-lauroylsarcosine Nervousness Neuroglia Neurons Penicillins Penicillin V Peptides phenethicillin Secretase sodium lauroyl sarcosinate Stem Cells Streptomycin
The individually collected datasets provided data on the total number of encounters with patients (that is, face to face consultations between the general practitioner and patients) and encounters for acute respiratory tract infections with diagnostic codes in terms of the International Classification of Primary Care (ICPC-2).24 All the general practitioners’ antibiotic prescriptions for acute respiratory tract infections were also included in the captured datasets.
During data analyses, we grouped together some diagnostic ICPC-2 codes reflecting similar illnesses: upper respiratory tract infections and respiratory symptoms (R01-05, 07-29, 74, and 80), ear infections (H01, 71, 72, and 74), and other respiratory tract infections (R71, 77, 82, and 83). Other included acute respiratory tract infections diagnoses were acute tonsillitis (R72 and 76), acute sinusitis (R75), acute bronchitis (R78), and pneumonia (R81).
In some of the analyses, we dichotomised antibiotics as penicillin V and non-penicillin V. We always analysed macrolides and lincosamides as one antibiotic group, because of similarities in microbiological effects and resistance mechanisms.25 (link) Lincosamides are rarely used as treatment for acute respiratory tract infections in Norwegian general practice, as macrolides account for 97% of this group.7 (link)
Our encounter/diagnosis data did not enable us to separate initial encounters from follow-ups. We therefore defined all encounters with an identical acute respiratory tract infection diagnostic code for an individual patient within a four week period as one episode of acute respiratory tract infection. If more than one antibiotic was prescribed during an episode, we selected the first prescription for our analyses.
We ran all statistical models in Stata 12. We evaluated the main intervention effects in multilevel logistic regression models including patient and general practitioner variables, comparing post-intervention data for the intervention and the control arms. The models included random intercepts for continuing medical education groups, general practitioners, and patients. Furthermore, the effects of patients’ sex, age, and diagnostic code were allowed to vary among general practitioners (that is, random slopes at the general practitioner level). The models were fitted by means of restricted maximum likelihood estimation, in which the estimations of (restricted) log-likelihoods were based on the laplacian approximation.26 All the included random effects (the intercepts and slopes mentioned above) were statistically significant. Estimates of effects of the intervention on individual variable groups were also based on post-intervention data. We estimated the intra-class correlation coefficients with the (xtmelogit) post-estimation function (estat icc) in Stata 12.
Publication 2013
Antibiotics Arm, Upper Bronchitis Diagnosis Ear Infection Education, Medical, Continuing Face Lincosamides Macrolides Patients Penicillins Penicillin V Pneumonia Primary Health Care Respiratory Tract Infections Signs and Symptoms, Respiratory Sinusitis Tonsillitis Upper Respiratory Infections
All cancer cell lines were obtained from the American Type Culture Collection (ATCC) except the UMRC6 cell line, which was provided by Dr. William G. Kaelin at the Dana-Farber Cancer Institute. All cell lines were free of Mycoplasma contamination (tested by the vendor). No cell line used in this study has been found in the International Cell Line Authentication Committee (ICLAC) database of commonly misidentified cell lines, based on short tandem repeat profiling performed by the vendor. Cells were cultured in Dulbecco modified Eagle’s medium (DMEM) with 10% (volume/volume; v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin in a 5% CO2 air atmosphere with 37 °C. For glucose or cystine deprivation experiments, cells were cultured in glucose-free and/or cystine-free DMEM with dialyzed fetal bovine serum as previously described22 -24 . Glucose-free DMEM was obtained from Life Technologies (#11966-025). Glucose- and cystine-free DMEM was customized from Athena Environmental Sciences. Cystine-free DMEM was subsequently prepared by adding back glucose.
Publication 2023
Atmosphere Cell Line Authentication Cell Lines Cells Cystine Eagle Fetal Bovine Serum Glucose Malignant Neoplasms Mycoplasma Penicillin V Short Tandem Repeat Streptomycin

Most recents protocols related to «Penicillin V»

All procedures were performed under a standardized surgical protocol. Patients were randomized to one of the two study intervention arms: IVRO group or SSRO group. Other orthognathic procedures were performed in a routine manner and sequence if indicated. The surgeries were performed under general anesthesia. The surgical procedures used in the two study arms are described below.
In the IVRO group, a vestibular incision was made at the buccal sulcus. A mucoperiosteal flap was raised to expose the lateral ramus. A sigmoid notch retractor and a posterior border retractor were placed for retraction. A vertical osteotomy from the sigmoid notch to the gonion of the mandible was made with an angled oscillating saw. The osteotomy was completed with a curved osteotome. The occlusion was achieved with an acrylic wager. No internal fixation was applied. IMF was secured with wire and custom-made arch bars for 6 weeks postoperative. The wound was closed with resorbable polyglactin sutures.
In the SSRO group, a standardized SSRO procedure with the Dal Pont-Hunsuck modification was performed. After vestibular incision at the buccal sulcus, a mucoperiosteal flap was raised. A short lingual osteotomy bisecting the upper and lower occlusal plane above the lingula was made. The anterior vertical osteotomy was made on the buccal cortex in the molar region. The lingual and buccal osteotomies were joined along the external oblique ridge. The osteotomies were completed with osteotomes and split to separate the proximal and distal segments. The buccal cortex was trimmed for segment adaptation. The occlusion was established with an acrylic wafer. One titanium miniplate was fixed on each side with four screws. The wounds were closed with resorbable polyglactin sutures. Light guiding elastics were used for 3–4 weeks postoperatively.
For both groups, routine medications including analgesics (paracetamol and codeine phosphate if not allergic), antibiotics (penicillin V if not allergic, for 2 days), and steroids (dexamethasone for 2 days) were given. Standard postoperative instructions were given in the same manner to both groups of patients.
Publication 2023
Acclimatization Acetaminophen Analgesics Antibiotics, Antitubercular Arm, Upper Codeine Phosphate Cortex, Cerebral Dental Occlusion Dexamethasone External Abdominal Oblique Muscle Fracture Fixation, Internal General Anesthesia Light Mandible Molar Occlusal Plane Operative Surgical Procedures Osteotomy Patients Penicillin V Pharmaceutical Preparations Poly(Lactide-Co-Glycoside) Sigmoid Colon Steroids Surgical Flaps Sutures Titanium Tongue Vestibular Labyrinth Vestibule of the Mouth Wounds
The cytotoxicity of A11 was evaluated by the 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay17 (link). L929 mouse fibroblast cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1% (v/v) of 100 IU/ml penicillin/streptomycin and 10% (v/v) fetal bovine serum (FBS). The cells were maintained in an air atmosphere containing the 5% CO2 under high humidity at 37 °C. L929 cell lines were seeded into a sterile 96-well plate (1 × 104 cells/well) then incubated overnight and treated with different concentrations of A11 (0.98–250 µg/ml) for 24 h. Media were removed, and cultured cells were incubated with 100 µl of MTT solution (4 mg/ml) for 4 h at 37 °C. After removal of MTT solution, the formed formazan was dissolved in 100 µl DMSO. Absorbance was measured by microplate reader at 570 nm; untreated cells served as negative control and melittin-treated cells as a positive control. Cell viability was determined following this equation: Cell viability%=ODTreated/ODControl×100
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Publication 2023
Atmosphere Bromides Cells Cell Survival Cultured Cells Culture Media Cytotoxin Eagle Fetal Bovine Serum Fibroblasts Formazans Humidity L929 Cells Melitten Mus Penicillin V Sterility, Reproductive Streptomycin Sulfoxide, Dimethyl
The hDPCs were derived from healthy third molars. The pulp tissue was minced with sterile scissors and subjected to enzymatic digestion with 1 mg/mL type-I collagenase for 30 min and tiled on the bottom of a culture bottle52 (link). The DPCs were cultured in α-MEM supplemented with 10% FBS and 1% (v/v) penicillin/streptomycin solution at 37 °C in 5% CO2. The vimentin expression was analyzed by immunofluorescence staining following the manufacturer’s protocol. Immunostaining was performed using primary antibodies against vimentin and the secondary antibody Alexa Fluor 488. After counterstaining the nuclei with the DAPI reagent, the cells were examined under a fluorescence microscope (Leica Optical, Germany). The hDPCs in passages 2–5 were used for the experiments.
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Publication 2023
alexa fluor 488 Antibodies Cell Nucleus Cells Collagenase, Clostridium histolyticum collagenase 1 DAPI Dental Pulp Digestion Enzymes Fluorescent Antibody Technique Immunoglobulins Microscopy, Fluorescence miltefosine Penicillin V Sterility, Reproductive Streptomycin Third Molars Tissues Vimentin Vision
U87 (brain-like glioblastoma), A172 (glioblastoma), and H4 (neuroglioma) cell lines were generously supplied by Professor Carla Vitorino (Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal). U118 (astrocytoma/glioblastoma) and U373 (astrocytoma/glioblastoma) cell lines were kindly gifted by Professor Maria Conceição Pedroso de Lima (Faculty of Sciences and Technology, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal). HEK-293 (immortalized human embryonic kidney fibroblasts) was kindly provided by Professor Paulo Oliveira (Institute for Interdisciplinary Research, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal). Cells were cultivated in Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM-HG) (Biowest, Nuaille’, France) supplemented with 10% (v/v) of heat-inactivated fetal bovine serum (FBS) (Sigma, St. Louis, MO, USA), and 1% (v/v) of penicillin-streptomycin (Sigma, St. Louis, MO, USA). Cells were maintained in a humidified atmosphere at 37 °C and 5% CO2. Throughout the experiments, cells were used at 80% confluence, and monitored by microscope observation to detect any morphological changes.
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Publication 2023
Astrocytoma Atmosphere Brain Cell Lines Cells Eagle Embryo Faculty Faculty, Pharmacy Fetal Bovine Serum Fibroblasts Glioblastoma Glucose Homo sapiens Kidney Microscopy Penicillin V Streptomycin
For the splice-correction assay, two human beta-thalassemia reporter cell lines, i.e., HeLa pLuc 705 [52 (link)] and HeLa EGFP 654 [53 (link),54 ], were used. For the gene-silencing assay, a luciferase-expressing cell line, i.e., U87 MG-Luc2 [55 ], was used. The cells were cultured at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) (Corning, Corning, NY, USA) containing 4.5 g/L of glucose and supplemented with 10% (v:v) of fetal bovine serum (FBS) and 1% (v:v) of a penicillin–streptomycin solution (100 U/mL penicillin and 100 g/mL streptomycin). The cells were grown on 6 cm cell culture dishes (Corning) in a humid atmosphere that contained 5% CO2. The cells were split every second day. To detatch the cells, trypsin-EDTA solution (Corning) was used. The cells were washed with Dulbecco’s Phosphate-Buffered Saline (DPBS) without calcium and magnesium (Corning). To conduct the experiments, the cells were plated on 96-well (Sarstedt, Nümbrecht, Germany) or 24-well (CytoOne, Hamburg, Germany) plates. All of the operations using cells were performed in a sterile cell culture hood.
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Publication 2023
Atmosphere beta Thalassemia Biological Assay Calcium Cell Culture Techniques Cell Lines Cells Culture Media Eagle Edetic Acid Fetal Bovine Serum Glucose HeLa Cells Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Luciferases Magnesium Penicillins Penicillin V Phosphates Saline Solution Sterility, Reproductive Streptomycin Trypsin

Top products related to «Penicillin V»

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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FBS, or Fetal Bovine Serum, is a commonly used cell culture supplement. It is derived from the blood of bovine fetuses and provides essential growth factors, hormones, and other nutrients to support the growth and proliferation of a wide range of cell types in vitro.
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Penicillin/streptomycin is a commonly used antibiotic mixture for cell culture applications. It provides broad-spectrum antimicrobial activity to prevent bacterial contamination in cell culture experiments.
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Streptomycin is a laboratory product manufactured by Merck Group. It is an antibiotic used in research applications.
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
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Penicillin is a type of antibacterial drug that is widely used in medical and laboratory settings. It is a naturally occurring substance produced by certain fungi, and it is effective against a variety of bacterial infections. Penicillin works by inhibiting the growth and reproduction of bacteria, making it a valuable tool for researchers and medical professionals.

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