All C. albicans strains used in this study are listed in Table 1 . Strains were grown at 30°C in YPD medium (1% yeast extract, 2% peptone, 2% glucose) or SD minimal medium [0.67% yeast nitrogen base (YNB; Difco) with 0.4 or 2% glucose] supplemented if necessary with arginine, histidine and uridine, at 20 mg.L− 1 and 2% agar for solid media. OE from PPCK1 was triggered in YNB plus 2% casamino acids liquid cultures at 30°C whereas OE from PTET was induced by the addition of 50 µg.mL− 1 doxycycline (Dox - Fluka) or 3 µg.mL− 1 anhydrotetracycline (ATc - Fisher Bioblock Scientific) in YPD at 30°C. ATc was preferred over Dox as this semi-synthetic tetracycline derivative has been described for its lower toxicity and its higher efficiency in the binding of the TetR repressor protein [92] (link). Furthermore, we have observed that 2 µg.mL− 1 ATc reproduced the effect of 50 µg.mL− 1 Dox, either on solid or in liquid medium and this concentration was not deleterious for growth or morphogenesis of C. albicans (Fig. S1 and data not shown). Dox- and ATc-containing cultures were maintained in the dark as these compounds are light sensitive.
Plasmids harbouring a Gateway® cassette were propagated in Escherichia coli strain TOP10 ccdBR (Invitrogen). Other plasmids were propagated in E. coli strain DH5α [93] (link). E. coli strains were grown in LB medium. Antibiotics were used at the following concentrations: ticarcillin, 50 µg.mL− 1; gentamycin, 10 µg.mL− 1; chloramphenicol, 15 µg.mL− 1.
Plasmids harbouring a Gateway® cassette were propagated in Escherichia coli strain TOP10 ccdBR (Invitrogen). Other plasmids were propagated in E. coli strain DH5α [93] (link). E. coli strains were grown in LB medium. Antibiotics were used at the following concentrations: ticarcillin, 50 µg.mL
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