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Ceramides

Ceramides are a class of lipid molecules composed of a sphingoid base and a fatty acid.
They are important structural components of cell membranes and play a key role in various biological processes, such as cell signaling, apoptosis, and skin barrier function.
PubCompare.ai's AI-driven platform can enhance the reproducibility and accuracy of Ceramides research by helping users find the best protocols and products through a seamless comparison of data from literature, pre-prints, and patents.
Explore this comprehensive solution to improve your Ceramides research today.

Most cited protocols related to «Ceramides»

1
Lipid Extraction from Diluted Plasma
The lipid extraction (adapted from Matyash et al. 23 (link)) was carried out in high grade polypropylene deep well plates. Fifty microliters of diluted plasma (50×) (equivalent of 1 μL of undiluted plasma) was mixed with 130 μL of ammonium bicarbonate solution and 810 μL of methyl tert-butyl ether/methanol (7:2, v/v) solution was added. Twenty-one microliters of internal standard mixture was pre-mixed with the organic solvents mixture. The internal standard mixture contained: 50 pmol of lysophasphatidylglycerol (LPG) 17:1, 50 pmol of lysophosphatic acid (LPA) 17:0, 500 pmol of phosphatidylcholine (PC) 17:0/17:0, 30 pmol of hexosylceramide (HexCer) 18:1;2/12:0, 50 pmol of phosphatidylserine (PS) 17:0/17:0, 50 pmol of phosphatidylglycerol (PG) 17:0/17:0, 50 pmol of phosphatic acid (PA) 17:0/17:0, 50 pmol of lysophposphatidylinositol (LPI 17:1), 50 pmol of lysophosphatidylserine (LPS) 17:1, 1 nmol cholesterol (Chol) D6, 100 pmol of diacylglycerol (DAG) 17:0/17:0, 50 pmol of triacylglycerol (TAG) 17:0/17:0/17:0, 50 pmol of ceramide (Cer) 18:1;2/17:0, 200 pmol of sphingomyelin (SM) 18:1;2/12:0, 50 pmol of lysophosphatidylcholine (LPC) 12:0, 30 pmol of lysophosphatidylethanolamine (LPE) 17:1, 50 pmol of phosphatidylethanolamine (PE) 17:0/17:0, 100 pmol of cholesterol ester (CE) 20:0, 50 pmol of phosphatidylinositol (PI) 16:0/16:0. The plate was then sealed with a teflon-coated lid, shaken at 4°C for 15 min, and spun down (3000 g, 5 min) to facilitate separation of the liquid phases and clean-up of the upper organic phase. Hundred microliters of the organic phase was transferred to an infusion plate and dried in a speed vacuum concentrator. Dried lipids were re-suspended in 40 μL of 7.5 mM ammonium acetate in chloroform/methanol/propanol (1:2:4, v/v/v) and the wells were sealed with an aluminum foil to avoid evaporation and contamination during infusion. All liquid handling steps were performed using Hamilton STARlet robotic platform with the Anti Droplet Control feature for organic solvents pipetting.
Surma M.A., Herzog R., Vasilj A., Klose C., Christinat N., Morin-Rivron D., Simons K., Masoodi M, & Sampaio J.L. (2015). An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids. European Journal of Lipid Science and Technology, 117(10), 1540-1549.
Publication 2015
1-Propanol Acids Aluminum ammonium acetate ammonium bicarbonate Ceramides Chloroform Cholesterol Cholesterol Esters Diacylglycerol Lipids Lysophosphatidylcholines lysophosphatidylethanolamine lysophosphatidylserine Methanol methyl tert-butyl ether Phosphates Phosphatidylcholines phosphatidylethanolamine Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Plasma Polypropylenes Solvents Sphingomyelins Teflon Triglycerides Vacuum
2
Mass Spectrometry Analysis of Lipid Extracts
Lipid extracts were dissolved in 60 μl of chloroform/methanol (1:2, v/v) and subjected to mass spectrometric analysis using an LTQ Orbitrap XL instrument (Thermo Fisher Scientific) equipped with a TriVersa NanoMate (Advion Biosciences) as previously described [4 (link),7 (link)]. The 10:1-phase lipid extracts were analyzed by positive ion mode multiplexed FT MS analysis with scan ranges m/z 280-580 (monitoring lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) species) and m/z 500-1200 (monitoring sphingomyelin (SM), ceramide (Cer), diacylglycerol (DAG), PC, ether-linked PC (PC O-), phosphatidylethanolamine (PE), ether-linked phosphatidylethanolamine (PE O-) and triacylglycerol (TAG) species). The 2:1-phase lipid extracts were analyzed by negative ion mode multiplexed FT MS analysis with scan ranges m/z 370-660 (monitoring lysophosphatidic acid (LPA), lysophosphatidylserine (LPS) and lysophosphatidylinositol (LPI) species) and m/z 550-1700 (monitoring phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG) and sulfatide (SHexCer) species). All FT MS spectra were acquired in profile mode using a target mass resolution of 100,000 (fwhm), activation of isolation waveforms, automatic gain control at 1e6, max injection time at 250 ms and acquisition of 2 µscans.
Husen P., Tarasov K., Katafiasz M., Sokol E., Vogt J., Baumgart J., Nitsch R., Ekroos K, & Ejsing C.S. (2013). Analysis of Lipid Experiments (ALEX): A Software Framework for Analysis of High-Resolution Shotgun Lipidomics Data. PLoS ONE, 8(11), e79736.
Publication 2013
Ceramides Chloroform Diacylglycerol Ethyl Ether isolation Lipids lysophosphatidic acid Lysophosphatidylcholines lysophosphatidylethanolamine lysophosphatidylinositol lysophosphatidylserine M 280 Mass Spectrometry Methanol Phosphatidic Acid phosphatidylethanolamine Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Radionuclide Imaging Sphingomyelins Sulfoglycosphingolipids Triglycerides
3
Lipid Annotation and Quantification
Lipid classes are: PE, phosphatidylethanolamines; LPE; lyso-phosphatidylethanolamines; PE-O, 1-alkyl-2-acylglycerophosphoethanolamines; PS, phosphatidylserines; PC, phosphatidylcholines; PC-O, 1-alkyl-2-acylglycerophosphocholines; LPC, lysophosphatidylcholines; SM, sphingomyelins; PA, phosphatidic acids; PG, phosphatidylglycerols; PI, phosphatidylinositols; DAG, diacylglycerols; TAG, triacylglycerols; CL, cardiolipins; LCL, triacyl-lysocardiolipins; Cer, ceramides; Chol, cholesterol; CholEst, cholesterol esters.
Individual molecular species are annotated as follows: :/:. For example, PC 18:0/18:1 stands for a phosphatidylcholine comprising the moieties stearic (18:0) and oleic (18:1) fatty acids. If the exact composition of fatty acid or fatty alcohol moieties is not known, the species are annotated as: :. In this way, PC 36:1 stands for a PC species having 36 carbon atoms and one double bond in both fatty acid moieties.
Herzog R., Schwudke D., Schuhmann K., Sampaio J.L., Bornstein S.R., Schroeder M, & Shevchenko A. (2011). A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biology, 12(1), R8.
Publication 2011
Carbon Cardiolipins Ceramides Cholesterol Cholesterol Esters Diglycerides Fatty Acids Fatty Alcohols Lipids Lysophosphatidylcholines Phosphatidic Acids Phosphatidylcholines Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylinositols Phosphatidylserines Sphingomyelins Triglycerides
4
Quantification of Endogenous Lipids in Mouse Tissues
Endogenous lipids from mouse liver and heart were detected and quantified using several techniques. FC was quantified using straight-phase HPLC and ELS detection as previously described10 (link). Quantification was made against an external calibration curve. This chromatographic set-up was also used to fractionate DG. Quantification of CE, TG, SM, and phospholipids (all from the total extract) and DG (fractionated from the HPLC) was made by direct infusion (shotgun) on a QTRAP 5500 mass spectrometer (Sciex, Concord, Canada) equipped with a robotic nanoflow ion source, TriVersa NanoMate (Advion BioSciences, Ithaca, NJ)11 (link). For this analysis, total lipid extracts, stored in chloroform:methanol (2:1), were diluted with internal standard-containing chloroform/methanol (1:2) with 5mM ammonium acetate and then infused directly into the mass spectrometer. The characteristic dehydrocholesterol fragment m/z 369.3 was selected for precursor ion scanning of CE in positive ion mode12 (link). The analysis of TG and DG was performed in positive ion mode by neutral loss detection of 10 common acyl fragments formed during collision induced dissociation13 (link). The PC, LPC and SM were detected using precursor ion scanning of m/z 184.114 (link), while the PE, phosphatidylserine (PS), phosphatidylglycerol (PG) and phosphatidylinositol (PI) lipid classes were detected using neutral loss of m/z 141.0, m/z 185.0, m/z 189.0 and m/z 277.0 respectively15 (link)16 (link). For quantification, lipid class-specific internal standards were used. The internal standards were either deuterated or contained diheptadecanoyl (C17:0) fatty acids.
Ceramides (CER), dihydroceramides (DiCER), glucosylceramides (GlcCER) and lactosylceramides (LacCER) were quantified using a QTRAP 5500 mass spectrometer equipped with a Rheos Allegro quaternary ultra-performance pump (Flux Instruments, Basel, Switzerland). Before analysis the total extract was exposed to alkaline hydrolysis (0.1M potassium hydroxide in methanol) to remove phospholipids that could potentially cause ion suppression effects. After hydrolysis the samples were reconstituted in chloroform:methanol:water [3:6:2] and analyzed as previously described17 (link).
For the recovery experiments the tissue samples were spiked with non-endogenously present lipids (or endogenous lipids spiked at relatively high levels) and could therefore all be detected by lipid class specific scans using the shotgun approach. In the recovery experiment we therefore also included the PA and phosphatidylcholine plasmalogen (PC P) lipid class, which we could not measure endogenously using our current analytical platform. Due to poor ionization efficiency, FC was derivatized and analyzed as picolinyl esters according to previous publication18 (link). See Table 1 for details. With some exceptions, lipids are annotated according to Liebisch et al.19 (link).
Löfgren L., Forsberg G.B, & Ståhlman M. (2016). The BUME method: a new rapid and simple chloroform-free method for total lipid extraction of animal tissue. Scientific Reports, 6, 27688.
Publication 2016
Allegro ammonium acetate Ceramides Chloroform Chromatography Dehydrocholesterols dihydroceramide Esters Fatty Acids Glucosylceramides Heart High-Performance Liquid Chromatographies Hydrolysis Lactosylceramides Lipids Liver Methanol Mice, House Phosphatidylcholines Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Phospholipids Plasmalogens potassium hydroxide Radionuclide Imaging Tissues
5
Simultaneous Sphingolipid and Ceramide Quantification
The concentration of the 10 sphingolipids and isotopic enrichment of [13C16]16:0-ceramide in muscle were simultaneously measured against an extracted concentration standard curve as well as an enrichment standard curve on a Thermo TSQ Quantum Ultra mass spectrometer (Waltham, MA) coupled with a Waters Acquity UPLC system (Milford, MA). The sphingolipids were separated on the UPLC with a Waters Acquity C8 UPLC BEH column 2.1 × 150 mm, 1.7 μm at 43°C using two buffers. Buffer A was methanol, 2 mM ammonium formate, 0.1% formic acid; buffer B was water, 1 mM ammonium formate, 0.1% formic acid. The flow rate was 0.4 ml/min, and the gradient conditions were as follows: 0 min at 20% B, 0–1.5 min 20-10% B, 1.5–2.3 min isocratic at 10% B, 2.3–9.3 min 10-1%B, 9.3–11 min isocratic at 1%B, 11–11.3 min 1–20%B, 11.3–13 min isocratic at 20%B. Standards and samples were re-suspended in 50 μl buffer A prior to injecting 5 μl onto the UPLC/MS/MS. Figure 1 shows the separation of all species in the standards (panel A) and muscle (panel B).
The mass spectrometer was equipped with an electrospray ionization interface. The following conditions were used: the spray voltage set at 4000V, sheath gas at 0.675 L/min, ion sweep gas at 0.6 L/min, aux gas at 1.2 L/min, and transfer capillary at 275°C. The collision gas was set at 1.2 mTorr. All sphingolipids, except C16:0-Cer, were monitored as [M+H]+ in positive mode. The C16:0-Cer and [13C16]16:0-Cer were monitored as [M+2+H]+ and [M+16+H]+ respectively. Transition of masses and collision energy are shown in Table 1. The entire analysis was performed in SRM mode.
Blachnio-Zabielska A.U., Persson X.M., Koutsari C., Zabielski P, & Jensen M.D. (2012). A liquid chromatography/tandem mass spectrometry method for measuring the in vivo incorporation of plasma free fatty acids into intramyocellular ceramides in humans. Rapid Communications in Mass Spectrometry, 26(9), 1134-1140.
Publication 2012
Buffers Capillaries Ceramides formic acid formic acid, ammonium salt GAS6 protein, human Isotopes Methanol Muscle Tissue Sphingolipids Tandem Mass Spectrometry

Most recents protocols related to «Ceramides»

1
Quantitative Liver Lipid Profiling
Extraction of liver lipids was performed according to the method described by Folch et al.30 (link). Dihydroceramides and ceramides and were isolated by solid phase extraction chromatography using C12:0 dihydroceramide and C17:0 ceramide (Avanti Polar Lipids, Alabaster, Al, USA) as internal standards. Samples were analysed by liquid chromatography-mass spectrometry (LC–MS) using a Thermo Exactive Orbitrap mass spectrometer (Thermo Scientific, Hemel Hempsted, UK) equipped with a heated electrospray ionization (HESI) probe and coupled to a Thermo Accela 1250 ultra-high-pressure liquid chromatography (UHPLC) system. Samples were injected onto a Thermo Hypersil Gold C18 column (2.1 mm by 100 mm; 1.9 μm) maintained at 50 °C. Mobile phase A consisted of water containing 10 mM ammonium formate and 0.1% (vol/vol) formic acid. Mobile phase B consisted of a 90:10 mixture of isopropanol-acetonitrile containing 10 mM ammonium formate and 0.1% (vol/vol) formic acid. The initial conditions for analysis were 65% mobile phase A, 35% mobile phase B and the percentage of mobile phase B was increased from 35 to 65% over 4 min, followed by 65% to 100% over 15 min, with a hold for 2 min before reequilibration to the starting conditions over 6 min. The flow rate was 400 μl/min and samples were analyzed in positive ion mode. The LC–MS data were processed with Thermo Xcalibur v2.1 (Thermo Scientific) with signals corresponding to the accurate mass-to-charge ratio (m/z) values for dihydroceramide and ceramide molecular species extracted from raw data sets with the mass error set to 5 ppm. Quantification was achieved by relating the peak area of the dihydroceramide and ceramide lipid species to the peak area of their respective internal standard. All values were normalised to the wet weight of liver.
Thompson D., Mahmood S., Morrice N., Kamli-Salino S., Dekeryte R., Hoffmann P.A., Doherty M.K., Whitfield P.D., Delibegović M, & Mody N. (2023). Fenretinide inhibits obesity and fatty liver disease but induces Smpd3 to increase serum ceramides and worsen atherosclerosis in LDLR−/− mice. Scientific Reports, 13, 3937.
Publication 2023
acetonitrile Alabaster Altretamine Ceramides Chromatography dihydroceramide formic acid formic acid, ammonium salt Gold High-Performance Liquid Chromatographies Isopropyl Alcohol Lipids Liquid Chromatography Liver Mass Spectrometry Solid Phase Extraction
2
Conformational Analysis of GCS Substrates
To
analyze the location and conformation of the GCS substrates, an FM
protocol was used.20 (link) A starting ceramide
conformation inside the enzyme was chosen between those found after
preliminary MD runs to be compatible with glycosylation, i.e., when
the distance dC1-Oc between the terminal
hydroxyl oxygen of the ceramide’s polar head and the carbon
C1 of glucose in UDP-glucose (atoms involved in the glycosylation
reaction were minimal). The starting system is shown in Figure S3. According to FM, biased CVs chosen
were the position along the rotation axis of the funnel (CV1), its
distance from the axis (CV2), and the distance dC1-Oc (CV3). The funnel parameters that were used are Zcc =
3.0 nm, Alpha = 0.5 rad, and Rcyl = 0.6
nm. Gaussian functions with a height of 2 kJ/mol and a sigma value
of 0.05 were deposited every 1 ps.
Canini G., Lo Cascio E., Della Longa S., Cecconi F, & Arcovito A. (2023). Human Glucosylceramide Synthase at Work as Provided by “In Silico” Molecular Docking, Molecular Dynamics, and Metadynamics. ACS Omega, 8(9), 8755-8765.
Publication 2023
Ceramides Enzymes Epistropheus Glucose Glycosylation Head Oxygen Uridine Diphosphate Glucose
3
Ceramide Modulates Microbial Growth
Lyophilized ceramide (esterified omega-hydroxyacyl-sphingosine; EOS) was purchased from Avanti Polar Lipids (USA). EOS was added to sterilized media at 45°C and placed on a magnetic stirrer. M. furfur and C. acnes were cultured on appropriate culture media discs, and their colonization patterns were observed. The concentrations of ceramide (EOS) were as follows: control (no additional treatment), 2, 5, and 10 μg/mL. Colony area analysis was performed using ImageJ software (13 (link)). The observed colony area values were divided by the colony area of the control (no EOS) and compared between different EOS treatments.
Statistical AnalysisThe Student’s t-test was used for dependent samples, and the nonparametric Mann–Whitney U test was used for comparison of quantitative values between two groups. The Kruskal–Wallis test was used to compare more than two groups. Tukey’s multiple comparisons test was used after Kruskal-Wallis test to compare each experimental group against each control group. Quantitative data are described as median and range or as mean ± standard deviation. Statistical differences were considered significant if p< 0.05. GraphPad Prism version 9.4.1 for Windows (GraphPad Software, San Diego, CA, USA) and SPSS (version 19.0; SPSS Inc., Chicago, IL, USA) were used to calculate statistical significance.
Chu H., Kim S.M., Zhang K., Wu Z., Lee H., Kim J.H., Kim H.L., Kim Y.R., Kim S.H., Kim W.J., Lee Y.W., Lee K.H., Liu K.H, & Park C.O. (2023). Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation. Frontiers in Immunology, 14, 1114321.
Publication 2023
Acne Vulgaris Ceramides Culture Media Lipids prisma Sphingosine Student
4
Abdominal Skin Biopsy Analysis
Full-thickness biopsies of abdominal skin, collected during cosmetic surgery, were purchased from suppliers accredited by the French Ministry of Research: Alphenyx (Marseille, France) and DermoBioTec (Lyon, France). The tissue collection used in this study included 3 biopsies of abdominal skin from 33-, 34- and 35-year-old women for histology, microarray analysis, IVL and lipid staining, and 3 from 36-, 47- and 49-year-old women for ceramide analysis.
HIEO and NA were solubilized in DMSO at 20% and 6%, respectively. DMSO never exceeded 0.5% in culture medium and was considered as the control.
Treatment consisted of DMSO at 0.5% as control, HIEO at 0.1% or NA at 0.03% in the culture medium at day 0 and day 2.
Quadruplicates from each donor were collected 24 hours after treatment for genomic expression analysis and at day 5 for morphological evaluation by histology, IVL detection, lipids and ceramides analysis.
Lemaire G., Olivero M., Rouquet V., Moga A., Pagnon A., Cenizo V, & Portes P. (2023). Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier. PLOS ONE, 18(3), e0268384.
Publication 2023
Abdomen Aftercare Biopsy Ceramides Culture Media Genome Lipids Microarray Analysis Skin Sulfoxide, Dimethyl Tissue Donors Woman
5
Ceramide Profiling in Epidermis by LC-MS
Ceramide content in the epidermis were extracted from tissues by a dual liquid–solid extraction method. The lipid solution was then completely dried under N2 at 30°C. Residues were re-suspended in 50 μL of a chloroform/methanol mixture. Ceramides were analyzed by a LC/MS Ultimate 3000 liquid chromatography system coupled to a MSQ Plus single quadrupole mass spectrometer (Thermo Fisher Scientific, Sunnyvale, CA).
In this LC/MS system, two mobile phases—M1 (methanol/water (50:50, v/v) and M2 (methanol/isopropanol (80:20, v/v))—were eluted at a flow rate of 0.2 mL/min. The mobile phases were programmed consecutively as follows: a linear gradient of 100–0% M1 between 0 and 20 mins, an isocratic elution of 0% M1 for 30 mins, and then an isocratic elution of 100% M1 for 10 mins. The injection volume was 20 μL and column temperature was maintained at 40°C. For MS detection, atmospheric pressure chemical ionization was used as the ion source.
Lemaire G., Olivero M., Rouquet V., Moga A., Pagnon A., Cenizo V, & Portes P. (2023). Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier. PLOS ONE, 18(3), e0268384.
Publication 2023
Atmospheric Pressure Ceramides Chloroform Epidermis Isopropyl Alcohol Lipids Liquid Chromatography Methanol Tissues

Top products related to «Ceramides»

1
Bodipy tr ceramide by Thermo Fisher Scientific
Sourced in United States
BODIPY TR Ceramide is a fluorescent lipid analogue that can be used to label cellular membranes. It is a derivative of the boron-dipyrromethene (BODIPY) dye family, characterized by its red-shifted excitation and emission spectra.
2
Sphingomyelin by Avanti Polar Lipids
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Sphingomyelin is a type of lipid found in cell membranes. It is a vital component of the myelin sheath, which insulates nerve fibers. Sphingomyelin plays a crucial role in maintaining the structural integrity and function of cell membranes.
3
Phosphatidylcholine by Avanti Polar Lipids
Sourced in United States
Phosphatidylcholine is a naturally occurring phospholipid that is a major component of cell membranes. It is a colorless, viscous liquid at room temperature. Phosphatidylcholine is a key structural element in biological membranes and plays a crucial role in cellular function and integrity.
4
Ammonium acetate by Merck Group
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Ammonium acetate is a chemical compound with the formula CH3COONH4. It is a colorless, crystalline solid that is soluble in water and alcohol. Ammonium acetate is commonly used in various laboratory applications, such as pH adjustment, buffer preparation, and as a mobile phase component in chromatography.
5
Ammonium formate by Merck Group
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Ammonium formate is a chemical compound that is commonly used in various laboratory applications. It is a crystalline solid that is soluble in water and other polar solvents. Ammonium formate serves as a buffer in analytical techniques and is also used as a mobile phase additive in liquid chromatography.
6
Chloroform by Merck Group
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Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
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Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
8
Bodipy fl c5 ceramide by Thermo Fisher Scientific
BODIPY FL C5 ceramide is a fluorescent lipid analogue used to label cellular membranes and study their dynamics. It consists of a BODIPY fluorescent dye attached to a five-carbon fatty acid chain, which allows it to incorporate into cellular lipid bilayers.
9
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

More about "Ceramides"

Ceramides are a class of lipid molecules composed of a sphingoid base and a fatty acid.
They are essential structural components of cell membranes and play a crucial role in various biological processes, such as cell signaling, apoptosis, and skin barrier function.
These lipid molecules are closely related to other lipid species like BODIPY TR Ceramide, Sphingomyelin, and Phosphatidylcholine.
Ceramide research can be enhanced through the use of PubCompare.ai's AI-driven platform, which helps users find the best protocols and products by seamlessly comparing data from literature, pre-prints, and patents.
This comprehensive solution can improve the reproducibility and accuracy of Ceramides research.
In addition to Ceramides, related compounds like Ammonium acetate, Ammonium formate, Chloroform, and Methanol are often used in the analysis and extraction of these lipid molecules.
The fluorescent probe BODIPY FL C5 ceramide can also be employed to study the localization and trafficking of Ceramides within cells.
By incorporating synonyms, related terms, abbreviations, and key subtopics, researchers can optimize their Ceramides-related content for search engines and enhance the discoverability of their work.
This informative and easy-to-read content can help advance the understanding and applications of these important lipid molecules.