Citric Acid
It plays a vital role in the Krebs cycle, and has a wide range of applications in food, beverage, pharmaceutical, and industrial sectors.
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Most cited protocols related to «Citric Acid»
Nanowells were prepared as described previously (Junesch et al., 2012 (link), 2015 (link); Malekian et al., 2017 (link); Ferhan et al., 2018 (link)) using 107 nm polystyrene colloids on Nb2O5 and 158 nm on SiO2 (Microparticles). Nanowells in SiO2 were prepared on fused silica to enable direct etching of the solid support (Malekian et al., 2017 (link)). Nanowells in Nb2O5 were prepared on borosilicate glass (which cannot be easily etched) onto which Nb2O5 was first deposited by reactive sputter coating with O2 and Ar (Junesch et al., 2012 (link)) (Nordiko). A 20 nm thick SiO2 layer was deposited by plasma enhanced chemical vapor deposition (Surface Technology Systems). Recipes aiming for stochiometric SiO2 or Si3N4 were used.
For bilayer formation with negative lipids, a 20 mM citric acid buffer was used with 150 mM KCl at a pH of 4.8. IRSp53 binding was performed in a buffer with 20 mM tris(hydroxymethyl) aminomethane and 150 mM NaCl with pH adjusted to 7.4 unless stated otherwise. The pH values were adjusted with concentrated HCl and NaOH.
The setup for extinction spectroscopy with high resolution and tracking of multiple resonance features has been described previously (Junesch et al., 2015 (link); Ferhan et al., 2018 (link)). Extinction is presented using the natural logarithm of the ratio between reference and measured intensities.
Liposomes were formed within 1 d of protein preparation by 30-h dialysis of micellar solutions containing E. coli polar lipid (Avanti, 20 mg/ml), detergent (Chaps, 35 mM), and protein (0.03–50 μg/mg lipid). Protein concentration is reported throughout as protein/lipid weight ratio, denoted “protein density.” Liposomes used for planar bilayer recording were prepared at a protein density of 50 μg/mg in 450 mM KCl, 25 mM KH2PO4, 22.5 mM K3-citrate, 2.5 mM citric acid, pH 7.5. Liposomes used for flux measurements were formed with protein at 0.03–5 μg/mg, 300 mM KCl, and buffered with 25 mM citrate for Cl− flux experiments or 25 mM citrate/ 25 mM phosphate (CPi) for H+ flux experiments, adjusted with NaOH to the desired pH in the range 4.5–5.5. (Some experiments used 75 mM glutamate as buffer, with similar results.) After dialysis, liposomes were stored in aliquots at −80°C until the day of use.
Most recents protocols related to «Citric Acid»
Example 4
A male 58-year-old subject suffering from a migraine ingested a capsule comprising 1000 mg citric acid and a capsule comprising 1200 mg KNO3, 200 mg elemental magnesium, and 50 mg elemental zinc. Within 5 minutes of ingesting both capsules, the subject saw alleviation of migraine symptoms. 30 minutes after ingesting the capsules, the subject reported that the migraine symptoms had disappeared.
Example 1
Exemplary capsule shell and matrix compositions useful for producing Liquisoft capsules as described herein are shown in Table 4. Composition components are set forth by weight percentage of the total weight of the composition. Such compositions may be encapsulated using rotary die encapsulation as described herein.
Formulas 1 and 2 were the first shell formulations developed to achieve faster disintegration time and prevent crosslinking of the gelatin shell with matrix fill components.
Example 10
30 mg of 6-phenoxyacetacetamidopenicillanic acid 2-dimethylaminoethyl ester hydrochloride, 30 mg of diethylaminoethyl acetylsalicylate hydrochloride, 30 mg of (RS)—N-[1-(1-benzothien-2-yl)ethyl]-N-(2-diethylaminoacetyloxyl)urea hydrochloride (an example of a HPP of zileuton), 15 mg of sildenafil citrate (an example of a compound having structure PDE5-I-1, wherein HA is citric acid), and 30 mg of isopropyl(±)-4-[1-hydroxy-4-[4-(hydroxydiphenylmethyl)-1-piperidinyl]-butyl]-α,α-dimethyl benzeneacetate hydrochloride (an example of a HPP of fexofenadine) in 0.5 ml of 25% ethanol was applied to the skin on the thorax of a subject every morning and evening (twice per day) until the condition was alleviated. Then 30 mg of diethylaminoethyl acetylsalicylate hydrochloride in 0.5 ml of water was applied to the skin on the thorax of a subject every morning and evening (twice per day) to prevent the recurrence of the condition.
EXAMPLE 7
Ticagrelor and pullulan were accurately weighed and dissolved in distilled water. This solution was mixed well followed by the addition of plasticizers and superdisintegrant. Then the resultant homogeneous solution was poured into a Petri dish (diameter 6 cm) and dried in an oven at 600 C for 24 h. The film was carefully removed from the Petri dish and cut into desired size (2×2 cm2).
Example 1
PGA Production via Compartmentalized Sequential Cultures of A. niger NRRL 599 and PGA Producing Bacillus sp. NRRL 14202O
PGA Producing Bacillus sp. NRRL 14202O was cultivated at bench scale on solid and liquid media prepared from supernatant (cell-free culture broth) from citric acid producing A. niger cultures grown on sucrose media (see scheme in
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More about "Citric Acid"
This versatile molecule plays a vital role in the Krebs cycle, a key metabolic pathway in living organisms.
Citric acid has a wide range of applications in the food, beverage, pharmaceutical, and industrial sectors, owing to its unique properties.
In the food industry, citric acid is commonly used as a flavoring agent, preservative, and pH regulator, contributing to the tangy, sour taste of many products.
It is also a key ingredient in the production of carbonated beverages, where it enhances the fizzy sensation.
Pharmaceutically, citric acid is employed in the formulation of various drugs, serving as an excipient, antioxidant, and pH modifier.
Beyond its biological and commercial applications, citric acid has a strong chemical presence.
It can be used in conjunction with other compounds, such as sodium hydroxide (NaOH), to create buffers or for pH adjustment.
Hydrochloric acid (HCl) and methanol are also commonly associated with citric acid, as they may be used in its extraction or purification processes.
Acetic acid and sodium chloride (NaCl) may also come into play in citric acid-related research and development.
Researchers exploring citric acid-related topics can greatly benefit from the AI-driven platform PubCompare.ai, which can optimize their research by easily locating relevant protocols from literature, preprints, and patents.
This cutting-edge technology leverages AI-powered comparisons to identify the most accurate and reproducible protocols and products, enhancing the efficiency and precision of citric acid-focused investigations.