The largest database of trusted experimental protocols

Cobalt

Cobalt is a hard, silvery-white metal that is essential for various biological processes.
It is a cofactor for numerous enzymes and plays a crucial role in the production of red blood cells and the maintenance of a healthy nervous system.
Cobalt is also used in the production of superalloys, magnetic materials, and rechargeable batteries.
Exposure to excessive levels of cobalt can lead to respiratory issues, skin irritation, and other health concerns.
Researchers studying cobalt-related topics can benefit from PubCompare.ai's AI-driven platform, which enhances reproducibility by helping identify the best protocols from the literature, preprints, and patents through streamlined, AI-powered comparisons.
This intuitive tool can improve the workflow of cobalt researchers and facilitate more efficient and reliable research.

Most cited protocols related to «Cobalt»

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2020
A-130A Buffers Cells Cloning Vectors Cobalt Freezing Hexosaminidase A His-His-His-His-His-His Histidine Phosphoric Monoester Hydrolases Proteins SARS-CoV-2 SDS-PAGE Severe acute respiratory syndrome-related coronavirus Signal Peptides Sodium Chloride Transfection Tromethamine

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2009
Blinking Cobalt Cornea Eye Fluorescein Laceration Light Mucus Neoplasm Metastasis Patients Sensitive Populations Slit Lamp Vision
The stabilized NTS1 mutant with T4 lysozyme replacing most of intracellular loop 3 was expressed in insect cells and purified by cobalt affinity chromatography. It was crystallized in lipidic cubic phase. Diffraction data were collected at the GM/CA-CAT beamline of the Advanced Photon Source at the Argonne National Laboratory. The structure was solved by molecular replacement using data from a single crystal.
Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.
Publication 2012
Cells Chromatography, Affinity Cobalt Cuboid Bone hen egg lysozyme Insecta Lipids Protoplasm
The current method is based on the concept of establishing a simple assay of catalase enzyme activity for biological tissues, which depends on the conversion of the oxidation state of cobalt (II) to cobalt (III) by hydrogen peroxide in the presence of bicarbonate solution. This process ends with the formation of a carbonato-cobaltate (III) complex ([Co (CO3)3]Co). This end product has two clear absorption peaks at 440 and 640 nm. The 440-nm band has been used for the assessment of catalase activity. Dissociation of hydrogen peroxide is proportional to the activity of catalase enzyme in the used sample. The method has been developed for the measurement of catalase activity in biological samples (bacteria, red blood cells, and liver and kidney tissue homogenates).
Publication 2018
Bacteria Biopharmaceuticals Catalase Cobalt enzyme activity Enzyme Assays Erythrocytes Ion, Bicarbonate Kidney Liver Peroxide, Hydrogen Tissues
Cerebral organoids were generated using a modified version of the protocol published by Lancaster et al.10 (link). Embryoid bodies (EBs) were derived by dissociating PSCs colonies and plating 2,500 single cells in each well of a 96-well plate. EBs were cultured as described in 10 (link). After five days in culture, EBs were transferred to Intermediate Induction Medium (IIM), consisting of DMEM/F12, 6% KOSR (Thermo Fisher Scientific), 0.9% FBS (GIBCO), 6 ng/ml bFGF (Peprotech), 0.7% N2 supplement (Invitrogen), Glutamax (Invitrogen), minimum-essential-media non-essential amino acids (MEM-NEAA) (Thermo Fisher Scientific), heparin 0.7 μg/ml (Sigma). Two days after plating in IIM, 500 μl of Neural Induction Medium (NIM) were added to each well. Following neural induction, organoids were embedded in Matrigel and transferred to cerebral differentiation medium (CDM) as described in10 (link). A total of 40 organoids were added to each spinner flask, and medium was changed once every six days for the duration of the culture process. After one month, brain derived neurotrophic factor (BDNF) was supplemented to the CDM medium at a concentration of 14 ng/ml. Cerebral organoids were cultured for up to 13 months. For induction of hypoxia, six-month organoids were cultured in cerebral differentiation medium supplemented with 100μM cobalt (II) chloride (Millipore) in a conventional incubator (37°C; 5% C02) for 24 hours before fixation.
Publication 2017
Amino Acids, Essential Cells Chlorides Cobalt Culture Media Embryoid Bodies Heparin Hypoxia matrigel Nervousness Neurotrophic Factor, Brain-Derived Organoids Pancreatic Stellate Cells

Most recents protocols related to «Cobalt»

Example 2

A reaction solution was prepared by dissolving manganese (II) acetylacetonate acetate (Mn(CH3COCHCOCH2)2) and Cobalt (II) acetate tetrahydrate (Co(CH3COO)2.4H2O) in a mixture of oleic acid (OLAC) and 1-octadecene. The reaction solution had a molar ratio of 4.5 mol OLAC: mol Metal (Mn+Co) and a combined metal concentration of 0.9 mmol Mn/mL of 1-octadecene. The reaction solution was heated to a temperature of 130° C. under flowing nitrogen and held at 130° C. for 60 minutes. The mixture was then heated under an inert atmosphere of nitrogen at a rate of 10° C./min to reflux (320° C.). The reaction mixture was held at 320° C. for 120 min. The reaction mixture was cooled under an inert atmosphere using a flow of RT air to cool the exterior of the reaction vessel. The nanoparticles were collected and purified via repeated washing and decanting/centrifugation steps using hexane as a hydrophobic solvent, and isopropanol as a counter solvent. The purified nanoparticles were dispersed in toluene. TEM images illustrated that the nanoparticles are rod-shaped, have an average length of 64.1 with a length distribution of 15% and an average width of 11.7 nanometers with a width distribution of 13%.

Patent 2024
1-octadecene Acetate acetyl acetonate ARID1A protein, human Atmosphere Blood Vessel Centrifugation Cobalt Isopropyl Alcohol Manganese Metals Molar n-hexane Nitrogen Oleic Acid Solvents Toluene
Not available on PMC !

Example 3

The catalyst C is prepared using the same support as that used for the catalyst A, and by carrying out the same steps as those performed to obtain the catalyst A, the only difference being that the calcining step is carried out in air at 400° C. for 4 hours in a traversed bed with an air velocity of 3 litres of air/gram of catalyst and per hour (l/g·h).

The final catalyst C has a total cobalt content of 20% by weight (the content of Co present in the spinel phase being included), i.e. 15% by weight of cobalt as active phase.

Patent 2024
Cobalt gamma-valerolactone spinell
Not available on PMC !

Example 2

The catalyst B is prepared using the same support as that used for the catalyst A, and by carrying out the same steps as those performed to obtain the catalyst A, the only difference being that the calcining step is carried out in air at 400° C. for 4 hours in a traversed bed with an air velocity of 2 litres of air/gram of catalyst and per hour (l/g·h).

The final catalyst B has a total cobalt content of 20% by weight (the content of Co present in the spinel phase being included), i.e. 15% by weight of cobalt as active phase.

Patent 2024
Cobalt gamma-valerolactone spinell
Not available on PMC !

Example 4

The catalyst D is prepared using the same support as that used for the catalyst A, and by carrying out the same steps as those performed to obtain the catalyst A, the only difference being that the calcining step is carried out in air at 400° C. for 4 hours in a traversed bed with an air velocity of 4 litres of air/gram of catalyst and per hour (l/g·h).

The final catalyst D has a total cobalt content of 20% by weight (the content of Co present in the spinel phase being included), i.e. 15% by weight of cobalt as active phase.

Patent 2024
Cobalt gamma-valerolactone spinell
After 7 days of exposure to Mt as described in the above procedures, corneal injury and the extent (area) of this injury were assessed by a corneal fluorescein staining test. In brief, 3% fluorescein was dropped into the conjunctival sac of rats, and 2 min later the cornea of each rat was detected by cobalt blue light irradiation with slit lamp, and photographed. Further, visible signs of corneal edema and corneal opacification were looked for using a slit lamp microscope, and photographs also obtained.
Publication 2023
Cobalt Cornea Cornea Injuries Edema, Corneal Fluorescein Injuries Light Radiotherapy Sac, Conjunctival Slit Lamp Slit Lamp Examination

Top products related to «Cobalt»

Sourced in United States, Germany
HisPur Cobalt Resin is a chromatography resin designed for the purification of proteins with a histidine (His) tag. It utilizes immobilized cobalt ions to selectively bind and capture His-tagged proteins from complex mixtures. The resin provides efficient protein capture and elution, enabling the purification of target proteins for various analytical and preparative applications.
Sourced in Germany, United States
Cobalt(II) nitrate hexahydrate is an inorganic chemical compound with the molecular formula Co(NO3)2·6H2O. It is a crystalline solid that is soluble in water and other polar solvents. The compound is used as a precursor for the synthesis of other cobalt-containing compounds and in various laboratory applications.
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
Sourced in United States, Germany
Cobalt (II) chloride hexahydrate is an inorganic compound with the chemical formula CoCl2·6H2O. It is a crystalline solid that appears red-pink in color. The compound is soluble in water and is commonly used as a laboratory reagent and in various industrial applications.
Sourced in Germany, United States, United Kingdom, Italy, India, France, China, Australia, Spain, Canada, Switzerland, Japan, Brazil, Poland, Sao Tome and Principe, Singapore, Chile, Malaysia, Belgium, Macao, Mexico, Ireland, Sweden, Indonesia, Pakistan, Romania, Czechia, Denmark, Hungary, Egypt, Israel, Portugal, Taiwan, Province of China, Austria, Thailand
Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
Sourced in United States
Cobalt-TALON resin is a chromatography resin designed for the purification of His-tagged proteins. It utilizes cobalt as the immobilized metal ion to interact with the histidine tag on the target protein, allowing for efficient and selective capture and purification.
Sourced in Germany, United States, United Kingdom, India, Italy, France, Spain, Australia, China, Poland, Switzerland, Canada, Ireland, Japan, Singapore, Sao Tome and Principe, Malaysia, Brazil, Hungary, Chile, Belgium, Denmark, Macao, Mexico, Sweden, Indonesia, Romania, Czechia, Egypt, Austria, Portugal, Netherlands, Greece, Panama, Kenya, Finland, Israel, Hong Kong, New Zealand, Norway
Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
Sourced in Germany, United States
Cobalt(II) chloride is an inorganic compound with the chemical formula CoCl2. It is a crystalline solid that is soluble in water and alcohol. Cobalt(II) chloride is commonly used in various laboratory applications, such as in the detection of water content and as a desiccant.
Sourced in United States, Germany, United Kingdom, Japan, China, India, Cameroon, Singapore, Belgium, Italy, Spain
Oleylamine is a chemical compound used as a surfactant, emulsifier, and lubricant in various industrial applications. It is a long-chain aliphatic amine with a hydrocarbon backbone and an amino group at one end. Oleylamine is commonly used in the formulation of lubricants, coatings, and personal care products.
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

More about "Cobalt"

Cobalt is a vital trace element that plays a crucial role in numerous biological processes.
This hard, silvery-white metal is an essential cofactor for a variety of enzymes, contributing to the production of red blood cells and the maintenance of a healthy nervous system.
In addition to its biological importance, cobalt is widely utilized in the manufacturing of superalloys, magnetic materials, and rechargeable batteries.
Researchers studying cobalt-related topics can benefit from the AI-driven platform offered by PubCompare.ai.
This intuitive tool enhances reproducibility by helping identify the best protocols from the literature, preprints, and patents through streamlined, AI-powered comparisons.
This can significantly improve the workflow of cobalt researchers and facilitate more efficient and reliable research.
Cobalt's unique properties and applications have led to its use in a variety of related products and compounds.
HisPur Cobalt Resin, for example, is a popular affinity resin used for the purification of histidine-tagged proteins.
Cobalt(II) nitrate hexahydrate is a common cobalt salt used in various applications, while Sodium hydroxide is often employed in the synthesis and processing of cobalt-containing materials.
Cobalt(II) chloride hexahydrate is another important cobalt compound, known for its use as a desiccant and in the production of cobalt-based pigments.
Ethanol and Cobalt-TALON resin are also commonly used in cobalt-related research and applications.
Hydrochloric acid and Cobalt(II) chloride are utilized in the extraction, purification, and characterization of cobalt, while Oleylamine and DMSO may be employed as solvents or reagents in cobalt-based studies and processes.
By understanding the diverse applications and related compounds of cobalt, researchers can better navigate the complex landscape of cobalt-related research and leverage the powerful tools offered by PubCompare.ai to enhance the reproducibility and efficiency of their work.