Lifespan assays were performed as described previously [12 (link)]. Briefly, wild-type (N2) and fat-6-overexpressing worms (IJ508 yhEx112 [ges-1p::fat-6::GFP, odr-1::RFP]) were maintained on Escherichia coli (OP50)-seeded nematode growth medium (NGM) agar plates at 20°C. Synchronized wild-type and fat-6-overexpressing animals were transferred onto OP50-seeded NGM agar plates containing 10 μM 5-fluoro-2′-deoxyuridine (FUdR, Sigma, St Louis, MO, USA) at day 1 adult stage to prevent progeny from hatching. Worms that did not respond to gentle touching by a platinum wire were counted as dead. Animals that crawled off the plates, ruptured, or burrowed were censored but included in the statistical analysis. The movement-capacity data were adopted from our previously published paper [9 (link)].
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