Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Biologically Active Substance > Dietary Advanced Glycation End Products

Dietary Advanced Glycation End Products

Dietary Advanced Glycation End Products (dAGEs) are a class of compounds formed through non-enzymatic reactions between sugars and proteins, lipids, or nucleic acids.
These compounds have been implicated in the development of various health conditions, including diabetes, cardiovascular disease, and neurodegenerative disorders.
Understanding the role of dAGEs in human health and disease is an active area of research.

Most cited protocols related to «Dietary Advanced Glycation End Products»

1
Diet-induced obesity and caloric restriction
Sixteen diet-induced obese (DIO – fed Research Diets, Inc. D12492i, 60% calories as fat = “HFD”), and sixteen control diet fed (Research Diets, Inc. D12450Bi, 10 % calories as fat = “CON”) C57BL/6J-male mice were obtained at 18 weeks of age from the Jackson Laboratory (Bar Harbor, ME). These animals had been fed these respective diets since 6 weeks of age. Individually housed animals from both diet groups were randomized to remain on the ad-lib diets (DIO-AL and CON-AL) or to be calorically restricted to decrease their body weight by ~20% over a 1–2 week period by twice daily feeding of reduced quantities (50% of ad-libitum intake) of their respective diets. After a 20 ±1% weight reduction, weight-reduced (DIO-WR and CON-WR) mice were provided calories sufficient to stabilize their weights for an additional 23 weeks. Nine weeks after initiation of the weight reduction protocol (Figure 1A, denoted day 0), body weights (daily for all mice) and food intake (FI; daily for WR and every two days for AL mice) were recorded for the next 93 days, except on days when mice were in the calorimeter. Metabolizable energy intake (MEI), defined as grams of food ingested per 24h (weighed using custom-made stainless steel feeding baskets that minimized spillage - Dieter Wenzel; Detmold, Germany) multiplied by the metabolizable energy for the respective diets (5.24 Kcal/g for high fat (HFD) and 3.85 Kcal/g for the control (CON) diet), was calculated from the FI measurements. The first day of this 93 day period is designated as day 0 (Figure 1A). The 93 days following day 0 are divided into 3 measurement periods (Figure 1A):
One DIO-AL mouse died during the study and data from one CON-AL mouse were not included due to malfunction of the calorimetry chamber. Aspects of this study have been described previously 7 . All protocols were approved by the Columbia University Institutional Animal Care and Use Committee.
On day 0, day 37, and following each 72-hour calorimetry period, body composition was determined using a Bruker Minispec mouse TD-NMR analyzer (Bruker Inc, Billerica MA) 8 (link). All body composition measurements were made at 8–9 am, before weight reduced animals were fed.
Ravussin Y., Gutman R., LeDuc C.A, & Leibel R.L. (2012). Estimating Energy Expenditure in mice using an Energy Balance Technique. International journal of obesity (2005), 37(3), 399-403.
Publication 2012
Animals Animals, Domestic Body Composition Calorimetry Diet Dietary Advanced Glycation End Products Eating Food Institutional Animal Care and Use Committees Males Measure, Body Mice, House Mice, Inbred C57BL Obesity Stainless Steel
2
Evaluating Anti-Obesity Compounds

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2012
Adult BLOOD Body Fat Body Weight Brain Calorimetry, Indirect Dietary Advanced Glycation End Products Eating Freezing Glucose Groin Immune Tolerance Insulin Sensitivity JD5037 Joint Dislocations Kidney Liver Males Mice, House Mice, Inbred C57BL Neck Obesity Pad, Fat Retroperitoneal Space SLV319 System, Endocrine Therapy, Diet Tube Feeding
3
Dietary Risk Factors and Disease Outcomes
For each diet–disease pair, we used data from published meta-analyses of prospective observational studies to estimate the relative risk of mortality and morbidity.21 (link) For diet–disease pairs for which evidence was only available on morbidity, we assumed that the estimated relative risks were also applied to mortality (appendix). Considering the relationship of diet and metabolic risk factors and the well established age trend of the relative risks of metabolic risks for cardiovascular disease and type 2 diabetes, we used the age trend of the relative risks of metabolic risk factors22 (link) to estimate the age-specific relative risk of dietary risks for cardiovascular disease and type 2 diabetes (appendix). To estimate the impact of sodium on outcomes, we first estimated the relationship between urinary sodium and change in systolic blood pressure, and then estimated the relationship between change in systolic blood pressure and disease outcomes.14 (link)
Health effects of dietary risks in 195 countries, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. (2019). Lancet (London, England), 393(10184), 1958-1972.
Full text: Click here
Publication 2019
Cardiovascular Diseases Diabetes Mellitus, Non-Insulin-Dependent Diet Dietary Advanced Glycation End Products Sodium Systolic Pressure Urine
4
Maternal High-Fat/High-Sugar Diet Effects on Fetal Metabolism
The Institutional Animal Care and Use Committee at the University of Texas Health Science Center San Antonio approved all protocols. Female C57BL/6J mice (n=80), proven breeders (one previous litter) and approximately 12 weeks old (The Jackson Laboratory, Bar Harbor, ME, USA) were housed 5 per cage under controlled conditions (25°C, 12-hour light/dark cycle). Starting at 13 weeks of age, animals were fed ad libitum with a control (D12489B, 10.6 kcal% fat) or high fat pellet diet (Western Diet D12079B, 41 kcal% fat) supplemented with ad libitum access to sucrose (20 %) solution (High Fat/High Sugar, HF/HS). The sucrose solution was supplemented with vitamins (Vitamin Mix V10001, 10 gm/ 4000 kcal) and minerals (Mineral Mix S10001, 35 gm/ 4000 kcal). Diets were purchased from Research Diets (New Brunswick, NJ, USA). All animals had free access to water. Daily food intake was determined by weighing remaining food at the end of each week and used to calculate daily caloric intake. Consumption of sucrose solution (20%) by the HF/HS group was recorded every day. When females on the HF/HS diet had increased 25% in body weight they, and age-matched females on control diet, were mated with males on control diet. The presence of a plug represented embryonic day (E) 0.5 and dams were maintained on the respective diets throughout gestation. At E18.5, dams were euthanized for collection of blood and tissue samples or animals were anesthetized and a glucose tolerance test was performed.
Rosario F.J., Kanai Y., Powell T.L, & Jansson T. (2015). Increased placental nutrient transport in a novel mouse model of maternal obesity with fetal overgrowth. Obesity (Silver Spring, Md.), 23(8), 1663-1670.
Publication 2015
Animals BLOOD Body Weight Carbohydrates Diet Diet, High-Fat Dietary Advanced Glycation End Products Eating Embryo Females Food Glucose Tolerance Test Institutional Animal Care and Use Committees Males Mice, Inbred C57BL Minerals Pregnancy Sucrose Therapy, Diet Tissues Vitamins
5
Dietary AGE Impact on Mouse Physiology
All procedures involving mice were reviewed and approved by the Institutional Animal Care and Use Committee of University at Buffalo. Four week old C57BL/6NHsd male mice were purchased from Harlan Laboratories (Indianapolis, IN). These mice were individually caged and provided free access to water. Mice were divided into two groups: 1) regular AGE group, mice were fed with TestDiet 58G7 (TestDiet, Richmond, IN); 2) high AGE group, mice were fed with TestDiet 58G7 autoclaved at 120°C for 15 min, following a published method for the preparation of high AGE diet [23] (link). To monitor the growth of experimental groups, two mice fed ad lib with standard mouse chow (Teklad Global 18% Protein Rodent Diet, Harlan Laboratories) were used as reference animals. Fat contributed 12% of calories in TestDiet 58G7, compared to 18% in the standard mouse chow Teklad Global 18% Protein Rodent Diet. All mice in the study received the same calorie per gram body weight on a weekly basis.
Patel R., Baker S.S., Liu W., Desai S., Alkhouri R., Kozielski R., Mastrandrea L., Sarfraz A., Cai W., Vlassara H., Patel M.S., Baker R.D, & Zhu L. (2012). Effect of Dietary Advanced Glycation End Products on Mouse Liver. PLoS ONE, 7(4), e35143.
Full text: Click here
Publication 2012
Age Groups Animals Body Weight Buffaloes Dietary Advanced Glycation End Products Institutional Animal Care and Use Committees Males Mice, House Mice, Inbred C57BL Proteins Rodent Therapy, Diet

Most recents protocols related to «Dietary Advanced Glycation End Products»

1
Dietary Variability and Marine Contribution
To understand the dietary variability across age and sex and to estimate the marine contribution to the diet, which potentially influences the 87Sr/86Sr results, 25 inhumed individuals’ ribs were analysed for δ13C and δ15N. Collagen was extracted from the samples following the protocol of 14CHRONO Centre (QUB)50 . The C:Na ratio (ratio of carbon to nitrogen atoms) was determined on the same collagen by elemental analysis on the EA-IRMS51 (link). All extracts that contained C:Na ratios outside a range of 2.9–3.6 were not included since their isotopic composition may have been compromised by diagenetic alteration52 (link),53 (link).
Esposito C., Gigante M., Lugli F., Miranda P., Cavazzuti C., Sperduti A., Pacciarelli M., Stoddart S., Reimer P., Malone C., Bondioli L, & Müller W. (2023). Intense community dynamics in the pre-Roman frontier site of Fermo (ninth–fifth century BCE, Marche, central Italy) inferred from isotopic data. Scientific Reports, 13, 3632.
Full text: Click here
Publication 2023
Carbon Collagen Dietary Advanced Glycation End Products Isotopes Marines Nitrogen Ribs Therapy, Diet
2
Maternal Microbiota Impact on Offspring Growth
A total of 900 breeder eggs from each group were collected and stored in a 16 °C refrigerator over the last 5 days during the hen experimental period for hatching. The eggs were hatched using an automatic incubator (Yiai Electronic Technology Co., Ltd., Bengbu, China) with 50–70% humidity (1–6 days at 60–70%, 7–14 days at 50–55%, and 14–21 days at 65–70%) at 37.8 °C with intermittent rotation. Embryo mortality was recorded during hatching. Considering that the maternal microbiota might represent an influencing factor, the eggs were not disinfected before hatching. The hen feeding (described above) and breeder egg hatching experiments were conducted at Changchun Academy of Agricultural Science, Changchun, China.
After the chicks hatched, we randomly selected 30 healthy male and 30 healthy female offspring chicks with similar body weights of approximately 38 g from each group for further separate feeding. All chicks were fed a single basal diet until 21 days of age. The offspring chicks from the hens in the CON and CCAB groups were considered the cCON and cCCAB groups, respectively. We recorded growth performance (including body weight and tibial length) in these groups every week to assess the repeatability of our previous studies and to assess whether the sex of the offspring was an influencing factor. The detailed compositions of the basal diets given to the experimental chicks and the chick-brooding management information are listed in Additional file 1: Tables S1 and S2.
Gong H., Wang T., Wu M., Chu Q., Lan H., Lang W., Zhu L., Song Y., Zhou Y., Wen Q., Yu J., Wang B, & Zheng X. (2023). Maternal effects drive intestinal development beginning in the embryonic period on the basis of maternal immune and microbial transfer in chickens. Microbiome, 11, 41.
Full text: Click here
Publication 2023
Body Weight Diet Dietary Advanced Glycation End Products Embryo Humidity Males Microbial Community Tibia
3
Dietary Exposure Across Lifespan
Mothers were interviewed using a validated food frequency questionnaire (FFQ) of their diet at 26 wk gestation35 (link)–37 (link) and their child’s diet at age 5 y.38 (link),39 (link) Youth completed a validated food screener at 14 y40 (link) and basic diet questions at 18 y.41 ,42 In the FFQ administered during pregnancy, women were asked how often they ate various food items in the previous 3 months and how much they consumed each time. In the FFQ at 5 y, mothers were asked about the number of times their child ate various foods in the previous 4 wk. In the food screener at 14 y, the youth were asked the number of days in the previous week they ate or drank different food items and how much in 1 day. The validated FFQs and screener underwent proprietary data processing (Pregnancy35 (link)–37 (link) and 14-y FFQL Nutritionquest40 (link); 5-y FFQ: Harvard Nutrition Questionnaire Service Center)38 (link),39 (link),43 to convert reported dietary intake into summary variables and individual food items. We selected a priori those dietary variables that included foods commonly treated with glyphosate. We dichotomized continuous summary variables (i.e., total calories, total carbohydrates, whole grains, bran, fruits, and vegetables) into being above or below the median observed in our sample; we also dichotomized reported intake of individual foods (i.e., cold cereal, hot cereal, bread, tortillas, legumes) (e.g., <1 time per day vs. ≥1 time per day). In addition to the administration of the validated FFQs and screener, we asked mothers how frequently their 5-y-old children consumed fast food; we directly asked young adults this question at the 14-y and 18-y visits. We asked the 14- and 18-y-olds to report on their overall alcohol consumption; for 18-y-olds, we also asked about recent binge drinking ( ≥4 drinks in a row for females, ≥5 for males). At the 5-, 14-, and 18-y visits, we queried the mothers about family food security (U.S. Department of Agriculture Food Security Scale, Short Form).44
Eskenazi B., Gunier R.B., Rauch S., Kogut K., Perito E.R., Mendez X., Limbach C., Holland N., Bradman A., Harley K.G., Mills P.J, & Mora A.M. (2023). Association of Lifetime Exposure to Glyphosate and Aminomethylphosphonic Acid (AMPA) with Liver Inflammation and Metabolic Syndrome at Young Adulthood: Findings from the CHAMACOS Study. Environmental Health Perspectives, 131(3), 037001.
Publication 2023
Bread Carbohydrates Cereals Child Common Cold Diet Dietary Advanced Glycation End Products Eating Fabaceae Fast Foods Females Food Fruit glyphosate Males Mothers Pregnancy Vegetables Whole Grains Woman Young Adult Youth
4
Dietary Intervention and Exercise in Mice
Four-week-old healthy male C57BL/6J mice were purchased from GemPharmatech Co., Ltd. All mice were housed under standard laboratory conditions (12 h on/off) and in a temperature-controlled environment (22–24 °C) in the SPF Animal Research Center of Shanghai University of Sports (SYXK 2014-0002). The mice were given ad libitum access to food and water or fed a CR diet beginning at 8 weeks of age. The glucose fed to mice was tested three days before they were sacrificed. Mice were sacrificed 24 h after the last bout of EX. Mice in all groups were fasted for 6 h prior to tissue harvesting. Tissues were then rapidly dissected and processed or stored for analysis.
Feng Y., Cui Z., Lu X., Gong H., Liu X., Wang H., Cheng H., Gao H., Shi X., Li Y., Ye H., Zhang Q, & Kong X. (2023). Transcriptomics Dissection of Calorie Restriction and Exercise Training in Brown Adipose Tissue and Skeletal Muscle. Nutrients, 15(4), 1047.
Full text: Click here
Publication 2023
Dietary Advanced Glycation End Products Environment, Controlled Food Glucose Males Mice, House Mice, Inbred C57BL Tissues
5
Longitudinal Dietary Assessment in DAISY Cohort
Diet was measured using a Willett 111-item semi-quantitative food frequency questionnaire (FFQ) in the DAISY cohort [38 (link),39 (link)]. The FFQ was administered annually to the parents, inquiring on the previous year’s dietary intake of the child starting at 2 years of age, and was validated for use in the DAISY population [40 (link)]. After the age of 10, the children recalled their own diets by completing the youth/adolescent questionnaire (YAQ) [41 (link),42 (link)]. Both questionnaires produce similar estimates of the nutrients and may be combined for analyses, as reported [42 (link)]. As compared to a 3-day food record, the FFQ produced similar dietary patterns with a modest agreement in adolescents [43 (link)]. The nutrient values were residually adjusted for the total energy intake [44 (link)].
Buckner T., Johnson R.K., Vanderlinden L.A., Carry P.M., Romero A., Onengut-Gumuscu S., Chen W.M., Fiehn O., Frohnert B.I., Crume T., Perng W., Kechris K., Rewers M, & Norris J.M. (2023). An Oxylipin-Related Nutrient Pattern and Risk of Type 1 Diabetes in the Diabetes Autoimmunity Study in the Young (DAISY). Nutrients, 15(4), 945.
Full text: Click here
Publication 2023
Adolescent Child Diet Dietary Advanced Glycation End Products Food Nutrients Parent Youth

Top products related to «Dietary Advanced Glycation End Products»

1
Sas 9 by SAS Institute
Sourced in United States, Austria, Japan, Belgium, United Kingdom, Cameroon, China, Denmark, Canada, Israel, New Caledonia, Germany, Poland, India, France, Ireland, Australia
SAS 9.4 is an integrated software suite for advanced analytics, data management, and business intelligence. It provides a comprehensive platform for data analysis, modeling, and reporting. SAS 9.4 offers a wide range of capabilities, including data manipulation, statistical analysis, predictive modeling, and visual data exploration.
2
Td 88137 by Inotiv
Sourced in United States, United Kingdom, Montenegro
The TD.88137 is a laboratory equipment product from Inotiv. It is designed for use in scientific research and analysis applications. The core function of the TD.88137 is to perform a specific task or measurement, but a detailed description is not available while maintaining an unbiased and factual approach.
3
D12492 by Research Diets
Sourced in United States, China, Canada, Japan, Denmark, Montenegro, United Kingdom
The D12492 is a powdered rodent diet formulated by Research Diets. It is a highly palatable, nutrient-dense diet that provides a standardized nutritional profile for research purposes. The diet is designed to be easily administered and consumed by laboratory rodents.
4
C57bl 6j male mice by Jackson ImmunoResearch
Sourced in United States, Montenegro, Japan, United Kingdom
C57BL/6J male mice are a widely used inbred mouse strain. They are a valuable research model for a variety of studies, including immunology, genetics, and disease research.
5
Sas version 9 by SAS Institute
Sourced in United States, Austria, Japan, Cameroon, Germany, United Kingdom, Canada, Belgium, Israel, Denmark, Australia, New Caledonia, France, Argentina, Sweden, Ireland, India
SAS version 9.4 is a statistical software package. It provides tools for data management, analysis, and reporting. The software is designed to help users extract insights from data and make informed decisions.
6
Sas v9 by SAS Institute
Sourced in United States, Austria, United Kingdom, Cameroon, Belgium, Israel, Japan, Australia, France, Germany
SAS v9.4 is a software product developed by SAS Institute. It is a comprehensive data analysis and statistical software suite. The core function of SAS v9.4 is to provide users with tools for data management, analysis, and reporting.
7
Ain93ga 2 by Oriental Yeast
Sourced in Japan
The AIN93GA-2 is a laboratory equipment product designed for research and testing purposes. It serves as a specialized tool for conducting experiments and analyses. The core function of this equipment is to provide a controlled environment for various scientific applications, but its specific intended use is not included in this factual description.
8
Mcd diet by MP Biomedicals
Sourced in United States
The MCD diet is a specialized laboratory equipment designed to facilitate the induction and maintenance of a methionine-choline-deficient (MCD) diet in animal studies. This dietary intervention is commonly used to model liver disease and non-alcoholic fatty liver disease (NAFLD) in rodents. The MCD diet equipment enables researchers to precisely control the nutritional composition of the animal's diet, allowing for the study of the physiological and pathological effects of methionine and choline deficiency.
9
Balb c mice by Jackson ImmunoResearch
Sourced in United States, Montenegro, Canada, Japan, China
BALB/c mice are an inbred strain of albino laboratory mice. They are commonly used in immunological and cancer research due to their susceptibility to certain pathogens and tumors.
10
Spss statistical package by IBM
Sourced in United States, United Kingdom, Japan, Spain, Italy
SPSS is a software package developed by IBM that provides statistical analysis capabilities. It allows users to perform a wide range of statistical analyses, including data management, descriptive statistics, and advanced analytics. SPSS is designed to help users explore, visualize, and interpret data, though its specific use cases may vary depending on the needs of the user or organization.

More about "Dietary Advanced Glycation End Products"

Dietary Advanced Glycation End Products (dAGEs) are a class of compounds formed through non-enzymatic reactions between sugars and proteins, lipids, or nucleic acids.
These compounds, also known as glycotoxins or Maillard reaction products, have been linked to the development of various health conditions, including diabetes, cardiovascular disease, and neurodegenerative disorders.
Understanding the role of dAGEs in human health and disease is an active area of research.
Researchers are investigating the effects of dietary AGEs, such as those found in processed and high-heat-cooked foods, on the body.
Studies have shown that dAGEs can contribute to oxidative stress, inflammation, and insulin resistance, which are key factors in the pathogenesis of chronic diseases.
To study the impact of dAGEs, researchers may utilize animal models like C57BL/6J male mice or BALB/c mice, as well as diets like the AIN93GA-2 or MCD diet.
Statistical analysis of the data can be performed using software like SAS version 9.4 or the SPSS statistical package.
Advances in analytical techniques, such as those described in SAS 9.4 and TD.88137, have improved the identification and quantification of dAGEs in food and biological samples.
This, in turn, has facilitated a better understanding of the sources, absorption, and metabolism of these compounds in the body.
By exploring the complexities of dAGEs and their interactions with various physiological systems, researchers aim to develop strategies for mitigating the adverse effects of these compounds and promoting overall health and wellness.
The insights gained from this research can inform dietary recommendations, product development, and targeted interventions to address the growing burden of chronic diseases associated with dAGE exposure.