Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Biologically Active Substance > F2-Isoprostanes

F2-Isoprostanes

F2-Isoprostanes are a class of prostanoids formed by the free radical-catalyzed peroxidation of arachidonic acid.
They serve as sensitive and specific biomarkers of oxidative stress in vivo.
F2-Isoprostanes have been implicated in the pathogenesis of numerous disease states, including cardiovascular, neurodegenerative, and inflammatory conditions.
Optimizie your F2-Isoprostane research with PubCompare.ai's cutting-edge AI-driven tools for protocol comparison and optimization.
Discover the best protocols and products from literature, pre-prints, and patens to streamline your reasearch and maxamize your results.

Most cited protocols related to «F2-Isoprostanes»

1
Menstrual Cycle Oxidative Stress Dynamics
The overall objective of this study was to conduct a longitudinal assessment of the association of endogenous hormones with biomarkers of oxidative stress and antioxidant status during the menstrual cycle. There were four main objectives. The first was to study the intra-menstrual cycle variation of various measures of oxidative stress. This objective is intended to assess variation in several measures of oxidative stress during different phases of the menstrual cycle, including F2-8-isoprostanes in serum. Assessment of variation across individuals is planned. The second objective was to determine the relationship between specific reproductive hormone levels and oxidative stress during specific times in the menstrual cycle of premenopausal women. The panel of reproductive hormones in the blood that were of primary interest are oestradiol, progesterone, LH, FSH and sex hormone binding globulin (SHBG). The third objective was to examine the influence of external factors on both oxidative stress and hormone levels, and their interrelation. The study measured various biological factors that might influence oxidative stress, including serum concentration of certain antioxidant vitamin levels (retinoids, tocopherols, carotenoids and ascorbic acid). In addition, the study assessed other factors that might affect oxidative stress such as medication and supplement intake, cigarette smoking, alcohol consumption, dietary intake, physical activity and levels of stress. Lastly, the study was designed to evaluate the validity and reproducibility of the various biological markers included in the BioCycle study.
Wactawski-Wende J., Schisterman E.F., Hovey K.M., Howards P.P., Browne R.W., Hediger M., Liu A, & Trevisan M. (2009). BioCycle study: design of the longitudinal study of the oxidative stress and hormone variation during the menstrual cycle. Paediatric and perinatal epidemiology, 23(2), 171-184.
Publication 2009
Antioxidants Ascorbic Acid Biological Factors Biological Markers BLOOD Carotenoids Dietary Supplements Estradiol F2-Isoprostanes Hormones Menstrual Cycle Oxidative Stress Pharmaceutical Preparations Progesterone Reproduction Retinoids Serum Sex Hormone-Binding Globulin Tocopherol Vitamins Woman
2
Effects of Green Tea on Breast Cancer Risk
The primary objectives of this trial were to investigate the effects of consumption of green tea extract (GTE) containing 800 mg EGCG daily for one year on (i) mammographic density (ii) circulating estrone, estradiol, testosterone, androstenedione, and sex hormone binding globulin (SHBG) (iii) circulating insulin-like growth factor-1 (IGF-1) and IGF binding protein 3 (IGFBP-3) among healthy postmenopausal women at high risk of breast cancer due to dense breast tissue. We hypothesized that consumption of GTE would reduce mammographic density and circulating concentrations of IGF-1, estrone, estradiol, testosterone, and androstenedione, and increase blood levels of IGFBP-3 and SHBG, in directions associated with reduced breast cancer risk.
Secondary endpoints included urinary estrogen metabolites and circulating F2-isoprostanes. The MGTT also aimed to determine whether (i) the effect of GTE supplementation on the primary outcomes differs by COMT genotype and (ii) COMT genotypes alter tea catechin metabolism and urinary excretion. We hypothesized that the low (A/A) and intermediate (A/G) activity COMT genotypes would show the greatest response to catechin consumption and would have lower concentrations of urinary methylated catechins and methoxy estrogens, and higher circulating levels of unmethylated catechins.
This study was approved by the Institutional Review Boards (IRB) of the University of Minnesota, Park Nicollet Institute, the University of Southern California, and the University of Pittsburgh.
Samavat H., Dostal A.M., Wang R., Bedell S., Emory T.H., Ursin G., Torkelson C.J., Gross M.D., Le C.T., Yu M.C., Yang C.S., Yee D., Wu A.H., Yuan J.M, & Kurzer M.S. (2015). The Minnesota Green Tea Trial (MGTT), a randomized controlled trial of the efficacy of green tea extract on biomarkers of breast cancer risk: Study rationale, design, methods, and participant characteristics. Cancer causes & control : CCC, 26(10), 1405-1419.
Publication 2015
Androstenedione BLOOD Breast Catechin COMT protein, human epigallocatechin gallate Estradiol Estrogens Estrone Ethics Committees, Research F2-Isoprostanes Genotype Green Tea IGF1 protein, human IGFBP3 protein, human Malignant Neoplasm of Breast Metabolism Sex Hormone-Binding Globulin Testosterone Tissues Urine Woman
3
Quantification of Four F2-Isoprostane Isomers
Four isomers of F2-isoprostanes – iPF(2 alpha)-III, 2,3-dinor-iPF(2 alpha)-III, iPF(2 alpha)-VI, and 8,12-iso-iPF(2 alpha)-VI – were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) (12 (link), 13 (link)) on Shimadzu 20A series LC and Applied Biosystems API 4000 QTrap MS/MS instruments. Based on creatinine measurements, the urine samples were diluted to 0.65 mg/mL creatinine, and samples with creatinine levels equal to or below this value were analyzed without dilution. Sample preparation included addition of internal standards [iPF(2 alpha)-III-d4, 8,12-iso-iPF(2 alpha)-VI-d11, iPF(2 alpha)-VI-d4] and 10 μL 1M HCl; washing of samples (500 μL) with 1 mL hexane; extraction of the analytes by ethyl acetate/hexane mixture (3/1, v/v); evaporation of the liquid and resuspension of the residue in 150 μL of a mixture containing 70% mobile phase A (0.1% formic acid in water) and 30% methanol. The samples (100 μL) then were injected into the LC-MS/MS system. Two solid core C18 columns (Phenomenex Kinetex C18, 150 × 4.6 mm) in series were used to achieve chromatographic separation of the F2-isoprostane isomers. The mass spectrometer was operated in negative mode with the following MRM transitions (m/z): 353/193 [iPF(2 alpha)-III], 357/197 [iPF(2 alpha)-III-d4], 325/237 [2,3-dinor-iPF(2 alpha)-III], 353/115 [iPF(2 alpha)-VI and 8,12-iso-iPF(2 alpha)-VI], 364/115 [iPF(2 alpha)-VI-d11], and 357/115 [8,12-iso-iPF(2 alpha)-VI-d4]. Calibration samples covering the expected range of concentrations were prepared by adding pure material into pooled human urine, injected before and after the patient samples. Lower limits of quantification (LLOQ, >80 % accuracy) were 0.007, 0.34, 0.25, and 0.12 mg/mL for iPF(2 alpha)-III, 2,3-dinor-iPF(2 alpha)-III, iPF(2 alpha)-VI, and 8,12-iso-iPF(2 alpha)-VI, respectively.
Il'yasova D., Spasojevic I., Wang F., Tolun A.A., Base K., Young S.P., Marcom P.K., Marks J., Mixon G., DiGiulio R, & Millington D.S. (2010). Urinary Biomarkers of Oxidative Status in a Clinical Model of Oxidative Assault. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 19(6), 1506-1510.
Publication 2010
Chromatography Creatinine ethyl acetate F2-Isoprostanes formic acid Homo sapiens Isomerism Liquid Chromatography Methanol n-hexane Patients S-(2-(N,N-diisopropylamino)ethyl)isothiourea Tandem Mass Spectrometry Technique, Dilution Urine
4
Meta-Analysis of F2-Isoprostanes as Oxidative Stress Biomarkers

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2017
8-epi-prostaglandin F2alpha 8-isoprostaglandin F2alpha Amyotrophic Lateral Sclerosis Biological Markers Biopharmaceuticals Coagulation, Blood Epilepsy F2-Isoprostanes Homo Hyperthyroidism Isoprostanes Oxidative Stress PTGS2 protein, human Serum
5
Oxidative Stress in Schizophrenia Spectrum

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2016
Adult Age Groups Antipsychotic Agents Dementia Diagnosis Disabled Persons Epistropheus Ethanol F2-Isoprostanes Homo sapiens Intellectual Disability Nervous System Disorder Outpatients Protective Agents Schizoaffective Disorder Schizophrenia Tobacco Use Disorder

Most recents protocols related to «F2-Isoprostanes»

1
Quantitative Analysis of TNF-α and F2-Isoprostanes
The specimen for ELISA was collected from cardiac blood. The TNF-α analysis used serum, and F2-Isoprostanes used plasma for the analysis. The ELISA kit for the TNF-α analysis used the Sandwich-ELISA principle, while the ELISA kit for the F2-Isoprostanes analysis used the Competitive-ELISA principle. TNF-α and F2-Isoprostanes were evaluated using quantitative measurements.
Semita I.N., Utomo D.N, & Suroto H. (2023). Mechanism of spinal cord injury regeneration and the effect of human neural stem cells-secretome treatment in rat model. World Journal of Orthopedics, 14(2), 64-82.
Publication 2023
BLOOD Enzyme-Linked Immunosorbent Assay F2-Isoprostanes Heart Plasma Serum Tumor Necrosis Factor-alpha
2
Secretome-Mediated Spinal Cord Injury Recovery
The research was a proper experimental study. The Lemeshow formula counted the sample size (n = 15 rats), with correction factors of 20%. The rats were randomly grouped into the following three groups: Normal (15 experimental rats did not have SCI and did not get HNSCs-secretome), control (15 experimental rats did have SCI with physiologic saline), and treatment (15 experimental rats did have SCI with HNSCs-secretome) (Figure 1). The treatment group received a 30 μL HNSCs-secretome intrathecal injection in T10 three days after the SCI and laminectomy. Treatment and control groups were replicated 15 times, and we observed the study over 56 d. The study’s independent variable was HNSCs-secretome treatment, whereas the dependent variables were GDNF, BDNF, nestin, Bcl-2, VEGF, TGF-β, IL-10, MMP9, F2-Isoprostanes, TNF-α, NF-κB, locomotor function, and spinal cord lesion size.
Semita I.N., Utomo D.N, & Suroto H. (2023). Mechanism of spinal cord injury regeneration and the effect of human neural stem cells-secretome treatment in rat model. World Journal of Orthopedics, 14(2), 64-82.
Publication 2023
BCL2 protein, human F2-Isoprostanes Glial Cell Line-Derived Neurotrophic Factor IL10 protein, human Intrathecal Injection Laminectomy MMP9 protein, human Protein, Nestin Rattus norvegicus RELA protein, human Saline Solution Secretome Spinal Cord Transforming Growth Factor beta Tumor Necrosis Factor-alpha Vascular Endothelial Growth Factors
3
Dietary Intervention for Cognitive and Cardiometabolic Health in Elderly
The MedLey study was a dietitian-led, randomized, controlled, parallel dietary intervention trial comparing the effect of a traditional MedDiet with a habitual diet on cognitive and cardiometabolic health outcomes in a healthy elderly population. The protocol has been described elsewhere [17 ,18 (link)]. In brief, 152 healthy Australian men and women aged 65 years and above were recruited and randomly allocated to either a MedDiet or a habitual diet (HabDiet, control) for 6 months. A total of 137 participants completed the study (MedDiet n = 74, HabDiet n = 63). Outcome measures included blood pressure, anthropometry (BMI, body weight, abdominal adiposity as measured by dual energy X-ray absorptiometry (DEXA)), waist/hip ratio (WHR), endothelial function, F2-isoprostanes (F2-IsoP), inflammatory biomarkers, lipids, glucose, insulin, dietary compliance, and cognitive performance and were collected at baseline and at 3 and 6 months of the intervention. Participants attended fortnightly sessions with a study dietitian to ensure adherence was maintained. Dietary intake was assessed with a 3-day weighed food record (WFR) and a food frequency questionnaire (FFQ) [19 (link)] at baseline and then during each intervention phase at 2 months and 4 months. The MedDiet was based on the traditional Cretan MedDiet [20 (link)] and was rich in fruits, vegetables, extra virgin olive oil, legumes, nuts, grains, and cereals, with lower amounts of red meat, processed foods, and discretionary foods. WFR data were analysed using FoodWorks Professional software (Version 7.0.3016; Xyris Software) to generate grams and servings per day of foods and food groups [17 ]. These data were used to determine adherence to the dietary prescription and the calculation of DII and E-DII scores. The study was conducted according to the guidelines laid down in the Declaration of Helsinki, and all procedures involving human participants were approved by the Human Research Ethics Committee (22 June 2013, #31163), University of South Australia, Adelaide, Australia. Written informed consent was obtained from all participants before commencement. This trial was registered with the Australian New Zealand Clinical Trials Registry (www.anzctr.org.au (accessed on 8 December 2022)) as ACTRN12613000602729.
Clark J.S., Dyer K.A., Davis C.R., Shivappa N., Hébert J.R., Woodman R., Hodgson J.M, & Murphy K.J. (2023). Adherence to a Mediterranean Diet for 6 Months Improves the Dietary Inflammatory Index in a Western Population: Results from the MedLey Study. Nutrients, 15(2), 366.
Full text: Click here
Publication 2023
A 137 Biological Markers Blood Pressure Body Weight Cereals Cognition Diet Dietary Modification Dietitian Dual-Energy X-Ray Absorptiometry Endothelium Ethics Committees, Research F2-Isoprostanes Fabaceae Food Food, Processed Fruit Glucose Homo sapiens Inflammation Insulin Lipids Nuts Oil, Olive Population Health Red Meat Vegetables Waist-Hip Ratio Woman
4
HNSC-Secretome Impact on SCI Recovery
This research was a true experimental study. The sample size was calculated using the Lemeshow formula (n = 5 rats), with correction factors of 20%. The rats were randomly grouped into the following three groups: the treatment group (five experimental rats had SCI with HNSC-secretome), the control group (five experimental rats had SCI without HNSC-secretome), and the normal group (five experimental rats did not have SCI and did not get HNSC-secretome). The treatment group received a 30 µL HNSC-secretome intrathecal injection at the T10 level three days after the SCI and laminectomy [8 (link),14 (link)]. All groups were replicated five times. The authors observed the study over 28 days [15 (link)]. The independent variable of the study is HNSC-secretome, where as the dependent variables are neuropathic pain, locomotor function, TNF-α, F2-Isoprostane, MMP-9, TGF-β, and BDNF.
Semita I.N., Utomo D.N., Suroto H., Sudiana I.K, & Gandi P. (2023). The mechanism of human neural stem cell secretomes improves neuropathic pain and locomotor function in spinal cord injury rat models: through antioxidant, anti-inflammatory, anti-matrix degradation, and neurotrophic activities. The Korean Journal of Pain, 36(1), 72-83.
Publication 2023
F2-Isoprostanes Intrathecal Injection Laminectomy MMP9 protein, human Neuralgia Rattus norvegicus Secretome Transforming Growth Factor beta Tumor Necrosis Factor-alpha
5
Cardiac Blood ELISA for TNF-α and F2-Isoprostanes
The specimen for enzyme-linked immunosorbent assay (ELISA) was collected from the cardiac blood. The TNF-α analysis used the serum, and F2-Isoprostanes used plasma for analysis. The ELISA kit for the TNF-α analysis used the Sandwich-ELISA principle. The ELISA kit for the F2-Isoprostanes analysis used the Competitive-ELISA principle. TNF-α and F2-Isoprostanes were evaluated using quantitative measurements.
Semita I.N., Utomo D.N., Suroto H., Sudiana I.K, & Gandi P. (2023). The mechanism of human neural stem cell secretomes improves neuropathic pain and locomotor function in spinal cord injury rat models: through antioxidant, anti-inflammatory, anti-matrix degradation, and neurotrophic activities. The Korean Journal of Pain, 36(1), 72-83.
Publication 2023
BLOOD Enzyme-Linked Immunosorbent Assay F2-Isoprostanes Heart Plasma Serum Tumor Necrosis Factor-alpha

Top products related to «F2-Isoprostanes»

1
8 isopgf2a d4 by Cayman Chemical
8-isoPGF2a-d4 is a stable isotope-labeled internal standard used for the quantitation of 8-isoprostaglandin F2α (8-iso-PGF2α) in biological samples by mass spectrometry. It is a deuterated analog of 8-iso-PGF2α.
2
Biotek synergy h1 hybrid reader by Agilent Technologies
Sourced in United States
The BioTeK Synergy H1 Hybrid Reader is a multi-mode microplate reader that can perform absorbance, fluorescence, and luminescence detection. It is designed for a variety of applications, including cell-based assays, enzyme-linked immunosorbent assays (ELISAs), and nucleic acid quantification.
3
Cobas 6000 by Roche
Sourced in Switzerland, Germany, United States, Japan, Denmark, Italy, France, United Kingdom, Belgium, Norway, China, Israel, Poland, Netherlands, Sweden
The Cobas 6000 is an automated clinical chemistry and immunoassay analyzer system designed for high-volume laboratory testing. It combines the features of two separate instruments, the Cobas c 501 module for clinical chemistry and the Cobas e 601 module for immunoassays, into a single integrated platform. The Cobas 6000 system is capable of performing a wide range of diagnostic tests, including biochemical, immunological, and specialty assays.
4
Oxiselect 8 iso prostaglandin f2α elisa kit by Cell Biolabs
Sourced in United States
The OxiSelect 8-iso-Prostaglandin F2α ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of 8-iso-Prostaglandin F2α in biological samples.
5
4000 qtrap by Thermo Fisher Scientific
Sourced in United States, Canada, Japan
The 4000 QTRAP is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system designed for quantitative and qualitative analysis. It features a triple quadrupole mass analyzer and an enhanced sensitivity detector for accurate, reliable, and reproducible results.
6
Au5800 by Beckman Coulter
Sourced in United States, Japan, Germany, United Kingdom, China, Italy, Canada
The AU5800 is a chemistry analyzer designed for high-throughput clinical laboratory testing. It features advanced optics and automation to provide reliable and efficient sample processing. The core function of the AU5800 is to perform a variety of clinical chemistry tests, including immunoassays, on patient samples.
7
F2 isoprostanes by Cayman Chemical
Sourced in United States
F2-isoprostanes are a class of prostaglandin-like compounds that are formed by the free radical-catalyzed peroxidation of arachidonic acid. They serve as reliable markers of oxidative stress and lipid peroxidation in biological systems.
8
Eia kit by Cayman Chemical
Sourced in United States
The EIA kit is a laboratory tool used to detect and quantify specific molecules in a sample through enzyme-linked immunosorbent assay (ELISA) technique. It provides a standardized and reliable method for analyte measurement.
9
Agilent 6890n by Agilent Technologies
Sourced in United States, China, Germany, Canada, France, Italy, United Kingdom
The Agilent 6890N is a gas chromatograph (GC) designed for a variety of analytical applications. It features a dual-channel configuration, enabling simultaneous analysis of two separate samples. The instrument is equipped with electronic pneumatic controls for precise regulation of carrier gas flow and pressure. The 6890N is compatible with a range of detectors, such as flame ionization detectors (FID) and thermal conductivity detectors (TCD), to facilitate the identification and quantification of various chemical compounds.

More about "F2-Isoprostanes"

F2-Isoprostanes are a class of eicosanoid compounds formed by the free radical-catalyzed peroxidation of arachidonic acid, a polyunsaturated fatty acid.
These prostaglandin-like molecules serve as sensitive and specific biomarkers of oxidative stress in the body.
F2-isoprostanes have been implicated in the pathogenesis of various disease states, including cardiovascular conditions, neurodegenerative disorders, and inflammatory processes.
Optimizing F2-Isoprostane research can be achieved through the use of advanced analytical tools and techniques.
PubCompare.ai offers cutting-edge AI-driven tools for protocol comparison and optimization, allowing researchers to discover the best protocols and products from literature, pre-prints, and patents.
This can help streamline the research process and maximize the results.
For example, the 8-isoPGF2a-d4 internal standard is commonly used in the analysis of F2-isoprostanes, while the BioTeK Synergy H1 Hybrid Reader and Cobas 6000 analyzer are some of the instrumentation platforms employed.
The OxiSelect 8-iso-Prostaglandin F2α ELISA Kit and the 4000 QTRAP mass spectrometer are also valuable tools for the quantification and detection of F2-isoprostanes.
By leveraging the insights provided by PubCompare.ai's AI-driven tools, researchers can optimize their F2-Isoprostane research, streamline their workflows, and maximize the impact of their findings.
This can lead to advancements in the understanding and management of various disease states associated with oxidative stress.