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Genistein

Q: How can researchers effectively investigate the effects of Genistein?

Researchers utilize a variety of protocols and prodcuts to investigate the effects of Genistein in preclinical and clinical studies. PubCompare.ai can help optimize your Genistein research by providing easy access to relevant literature, preprints, and patents, while offering insightful comparisons to identify the most effective protocols and products. This can enhance the reproducibility and accuarcy of your Genistein studies.

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PubCompare.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform can help researchers identify the most effective protocols related to Genistein for their specific research goals. PubCompare.ai's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your Genistein studies.

Genistein is a naturally occurring isoflavone found in soybeans and other legumes.
It has been studied for its potential health benefits, including its antioxidant, anti-inflammatory, and anti-cancer properties.
Genistein may also play a role in regulating hormonal activity and bone health.
Researchers utilize a variety of protocols and prdoucts to investigate the effects of genistein in preclinical and clinical studies.
PubCompare.ai can help optimize your genistein research by providing easy access to relevant literature, preprints, and patents, while offering insightful comparisons to identify the most effective protocols and products.
This can enhance the reproducibility and accuracy of your genistein studies.

Most cited protocols related to «Genistein»

Proteome Analysis of Kaempferol Treatment

Cell lysates were obtained from 3T3-L1 or HepG2 cells. Cells were scraped and lysed with M-PER lysis buffer. After centrifugation for 15 min at 16,000×g, the supernatant was obtained, and protein content was quantified using Bradford reagent. Before drug treatment, the samples were diluted to achieve a protein concentration of 1 mg/mL. Samples were treated with the Kaem or DMSO for 2 h at 25 °C and then incubated with pronase (5, 10, and 20 µg/mL) or distilled water for 10 min at 25 °C. After the reaction, SDS was added to the sample and the samples were heated at 100 °C. A portion of each sample was used for LC–MS/MS analysis. Sample preparation and proteome analysis were conducted as indicated in the previous publication⁶⁹. For western blot analysis, VDAC1 or Na⁺K⁺ ATPase was used as an internal control. For the structure–activity-relationship (SAR) analysis, kaempferol (Sigma-Aldrich, 60010), Acacetin (Sigma-Aldrich, 00017), isosakuranetin (Sigma-Aldrich, PHL82569), Biochanin A (Sigma-Aldrich, D2016), (−)Epicatechin (Sigma-Aldrich, E4018), Genistein (Sigma-Aldrich, G6649) were used.

Kim D., Hwang H.Y., Ji E.S., Kim J.Y., Yoo J.S, & Kwon H.J. (2021). Activation of mitochondrial TUFM ameliorates metabolic dysregulation through coordinating autophagy induction. Communications Biology, 4, 1.

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Publication 2021

3T3-L1 Cells acacetin ATP8A2 protein, human biochanin A Buffers Cells Centrifugation Epicatechin Genistein Hep G2 Cells isosakuranetin kaempferol Na(+)-K(+)-Exchanging ATPase Pharmaceutical Preparations Pronase Proteins Proteome Sulfoxide, Dimethyl Tandem Mass Spectrometry VDAC1 protein, human Western Blot

Measurement of Environmental Exposures

Samples collected at visit 1 were analyzed at the National Center for Environmental Health laboratories at CDC for nine phthalate metabolites [n = 1,149; monoethylphthalate (MEP), mono-butyl phthalate, mono-iso-butyl phthalate, mono-benzyl phthalate, mono-3-carboxypropyl phthalate, mono-2-ethyl-5-carboxypentyl phthalate, mono-(2-ethyl-5-hydroxylhexyl) phthalate, mono-(2-ethyl-5-oxohexyl) phthalate, and mono-(2-ethylhexyl) phthalate (MEHP)], seven phenols (benzophenone-3, bisphenol A, 2,5-dichlorophenol, triclosan; n = 1,149; methyl-, butyl-, and propyl- parabens, n = 1,059), and three phytoestrogens (daidzein, genistein, enterolactone; n = 1,150). Parabens were not measured early in the study. At least one urinary biomarker measurement was available among 1,151 girls, 985 with breast stages. We substituted limit of detection ( for results below the LOD. Adjustment for urine dilution was accomplished using creatinine, to account for difference in sampling (spot specimens at MSSM and KPNC, early-morning samples at Cincinnati) and interindividual variation in urine dilution. We included log-creatinine in models using continuous log-biomarker variables, and we created quintile cut points from creatinine-corrected concentrations (micrograms per gram creatinine). As previously described, to reduce multiple comparisons we combined the phthalate metabolites into two groups that represent similar sources and similar biologic activity, low- (< 250 Da) and high-molecular-weight (> 250 Da) phthalate metabolites (low MWP and high MWP) [details in Supplemental Material, Table 2 (doi:10.1289/ehp.0901690)]. We expressed high MWP molar sum as MEHP (molecular weight 278) and the low MWP as MEP (molecular weight 194) so that units were the same as the other analytes (micrograms per liter). Similarly, a molar sum of the paraben metabolites was created (paraben sum) expressed as propyl paraben (molecular weight 180.2). Models with the individual phthalate and paraben metabolites were consistent with the molar sum variables. Results using di(2-ethylhexyl)phthalate (DEHP)-sum metabolites were almost identical to those for the high MWP, and they represented 75% ± 16% (mean ± SD) of the high MWP biomarkers. Therefore, only the latter models are presented.
Laboratory techniques and quality control protocols are identical to those reported previously in a pilot study (Wolff et al. 2007 (link)). Briefly, urine undergoes an automated cleanup with enzymatic deconjugation, followed by high-performance liquid chromatography-isotope dilution tandem mass spectrometry quantification (Kato et al. 2005 (link); Rybak et al. 2008 ; Ye et al. 2005 (link), 2006 ). In addition to the internal CDC quality control procedures, we incorporated approximately 10% masked quality control specimens (n = 101) from a single urine pool. The coefficients of variation (SD/mean concentration) were < 10% for 13 analytes and were between 10% and 21% for the remaining six biomarkers.

Wolff M.S., Teitelbaum S.L., Pinney S.M., Windham G., Liao L., Biro F., Kushi L.H., Erdmann C., Hiatt R.A., Rybak M.E, & Calafat A.M. (2010). Investigation of Relationships between Urinary Biomarkers of Phytoestrogens, Phthalates, and Phenols and Pubertal Stages in Girls. Environmental Health Perspectives, 118(7), 1039-1046.

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Publication 2010

2,3-bis(3'-hydroxybenzyl)butyrolactone Biological Markers Biopharmaceuticals bisphenol A Breast Creatinine daidzein Diethylhexyl Phthalate Enzymes Genistein High-Performance Liquid Chromatographies Isotopes Molar mono-(2-ethylhexyl)phthalate mono-benzyl phthalate mono-isobutyl phthalate monoethyl phthalate oxybenzone Parabens Phenols phthalate Phthalate, Dibutyl Phytoestrogens propylparaben Tandem Mass Spectrometry Technique, Dilution Triclosan Urine Woman

Quantifying CFTR Activity in Mutant Cells

Measurements of CFTR activity were carried out on CFBE41o-, FRT, and A549 cells expressing mutant F508del-CFTR and the HS-YFP 48 h after plating on microplates. Twenty-four hours after plating, the cells were incubated with test compounds at 37°C for 20 to 24 h. At the time of assay, cells were washed with PBS (containing 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, 1 mM CaCl₂, and 0.5 mM MgCl₂) and stimulated for 30 min with forskolin (20 μM) plus genistein (50 μM). Then, cells were transferred to a microplate reader (FluoStar Galaxy; BMG Labtech GmbH, Offenburg, Germany) for CFTR activity determination. The plate reader was equipped with high-quality excitation (HQ500/20X: 500 ± 10 nm) and emission (HQ535/30M: 535 ± 15 nm) filters for YFP (Chroma Technology Corp., Brattleboro, VT). Each assay consisted of a continuous 14-s fluorescence reading with 2 s before and 12 s after injection of an iodide-containing solution (PBS with Cl⁻ replaced by I⁻; final I⁻ concentration in the well: 100 mM). Data were normalized to the initial background-subtracted fluorescence. To determine fluorescence quenching rate (QR) associated with I⁻ influx, the final 11 s of the data for each well were fitted with an exponential function to extrapolate initial slope (dF/dt).

Sondo E., Tomati V., Caci E., Esposito A.I., Pfeffer U., Pedemonte N, & Galietta L.J. (2011). RESCUE OF THE MUTANT CFTR CHLORIDE CHANNEL BY PHARMACOLOGICAL CORRECTORS AND LOW TEMPERATURE ANALYZED BY GENE EXPRESSION PROFILING. American journal of physiology. Cell physiology, 301(4), C872-C885.

Publication 2011

A549 Cells Biological Assay Cells CFTR protein, human Colforsin Fluorescence Genistein Iodides Magnesium Chloride Sodium Chloride

Seasonal Dietary Intake Assessment Protocol

The subjects provided 7-day dietary records (DR) in 4 seasons (a total of 28 days): spring (May), summer (August), autumn (November) and winter (February). In Mito the PHC area, the study was launched in the spring of 1996, Half of the subjects from Chuo-higashi (n=32) joined the study in the summer of 1996, and the other half (n=44) in the winter of 1997. In other areas, the study began in winter of 1997.
Weighed DRs were collected over 7 consecutive days in each of the 4 seasons. Dietitians from the PHC, the cities or towns in each area instructed the subjects to weigh all foods and beverages using the measuring spoons, cups and an electronic scale provided, and to record them in a booklet especially designed for the purpose. The subjects gave detailed descriptions of each food, the method of preparation and names of the recipes. The dietitians checked the records at subjects' homes at least once during the survey.
At the end of each season, the dietitians from the PHC reviewed the records in a standardized way, and coded all the foods recorded according to the Standardized Tables of Food Composition, 4th edition,⁵ If codes were not available for certain local foods, the dietitians substituted the food considered to be most similar by asking subjects for details on the food. When ingredients were not obtained for any already prepared recipes, the standard recipes developed by the authors were used.
Nutrient and food calculation was done by the method used in the Cohort I validation study.^{6 (link)} The mean daily intake of energy and 16 nutrients was calculated from the records using the Standardized Tables of Food Composition, 4th edition.⁵ For cholesterol, and additional nutrients and compounds such as fatty acids (saturated, monounsaturated, n-3 polyunsaturated, n-6 polyunsaturated)^{7 (link)}, dietary fiber (water-soluble, -insoluble),^{8 (link)} selenium^{9 (link)} and carotenoids (alpha-carotene, beta-carotene, lycopene),^{10 (link)} the original food composition tables were developed by filling in the missing values for the Japanese composition tables. For isoflavones (daidzein and genistein), the values in the specially developed food composition table for isoflavones in Japanese foods were used.^{11 (link)}^,^{12 (link)}Additional information about the diet, the standard portions/units for rice and green tea, and brand names for usually used cooking oil, sugar, soy sauce and miso (fermented soybeans) were reported. The frequency of eating out and dietary supplement use for the week was also recorded. Name, age, sex and occupation of all members in the family, self-reported physical activity level, and the number of steps counted by pedometer for one arbitrary day in each season were reported for information on demographic status and physical activity.

Ishihara J., Sobue T., Yamamoto S., Yoshimi I., Sasaki S., Kobayashi M., Takahashi T., Iitoi Y., Akabane M, & Tsugane S. (2007). Validity and Reproducibility of a Self-administered Food Frequency Questionnaire in the JPHC Study Cohort II: Study Design, Participant Profile and Results in Comparison with Cohort I. Journal of Epidemiology, 13(1 Suppl), S134-S147.

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Publication 2003

alpha-carotene beta Carotene Beverages Carbohydrates Carotenoids Cholesterol daidzein Diet Dietary Fiber Dietary Supplements Dietitian Fatty Acids Food Genistein Green Tea Isoflavones Japanese Lycopene Miso Mitomycin Nutrients Oryza sativa Soybeans Soy Sauce

Isolation and Treatment of HNC-TICs

This study was approved by the Institutional Review Board of China Medical University Hospital (CSMUH: No: CS13250). HNC tissues were resected from two HNC patients who gave informed consent for the use of their tissues that were harvested at the surgery. To identify and ALDH1+CD44+ HNC-TICs and ALDH1-CD44- non-TICc, we stained cancer cells with ALDEFLUOR™ assay kit (StemCell Technologies, Vancouver, BC, Canada) and anti-CD44 antibody conjugated to phycoerythrin (BioLegend, San Diego, CA, USA) followed by fluorescence-activated cell sorting using FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). Smulow–Glickman (S-G) human gingival epithelial cells were originally derived from human attached gingiva [33 (link)].
Genistein (G-6649; Sigma, St Louis, MO, USA) and anti-oxidant NAC (N-acetyl-l-cysteine) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Genistein was dissolved in DMSO as a stock solution and diluted in culture medium to final concentrations (10–80 μM) prior to use.

Hsieh P.L., Liao Y.W., Hsieh C.W., Chen P.N, & Yu C.C. (2020). Soy Isoflavone Genistein Impedes Cancer Stemness and Mesenchymal Transition in Head and Neck Cancer through Activating miR-34a/RTCB Axis. Nutrients, 12(7), 1924.

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Publication 2020

Acetylcysteine Aldehyde Dehydrogenase 1 Family Antioxidants Biological Assay CD44 protein, human Cells Culture Media Epithelial Cells Ethics Committees, Research Genistein Gingiva Homo sapiens Immunoglobulins Malignant Neoplasms Operative Surgical Procedures Patients Phycoerythrin Stem Cells Sulfoxide, Dimethyl Tic Disorder Tissues